Document LJGaDEy7QE1zqdkY5VRNRw5ng

AR 226 -076/ ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) IESTSUBS~T ~~ANCE 00 Identity: Mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC203CF. Current information indicates itis a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether saits, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical componentof the test sample. METHOD: Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1991 Species: Daphnia magna Supplier: In-house cultures maintained at EnviroSystems Divisio, Resource Analysts, Inc., Hampton, NH. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: No calculations performed, based on study results. Test conditions: Dilution water: Filtered natural well water - Dilution water chemistry: Conductivity: 840 umhos/cm pH: 73 Lighting: Cool-white fluorescent lights at 20 pEin/sec/m?. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. _ Stock and test solution preparation: A primary 1000 mg. stock solution was prepared and added directly to create test solutions. Exposure vessels: 250 mL beakers containing 200 mL exposure solution at a water depth of approximately 6 cm. Number of replicates: 4 Number of daphnids per replicate: 5 001234 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 7.38.2 (control exposure) 7.5-8.3 (1000 mg/L exposure) Temperature range (0-48 hours): 19.1 19.6 C (control exposure) 19.1 -19.6 C (1000 mg/L exposure) Conductivity range (048 hours): 840 -- 860 umhos/om (control exposure) 860 ~ 870 umhos/cm (1000 mg/L exposure) Dissolved oxygen range (048 hours): 8.39.2 mg/L (control exposure) 8.39.2 mg/L (1000 mg/L exposure) Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L. Element values: 48-hour ECso = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. conclusions 0000 The test substance 48-hour ECso was determined to be >1000 mg/L. No `mortality or sublethal effects were observed. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATAQUALTY Reliability: Kiimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmationofthe amountoffluorochemical proportion in the solution. ne This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-3, 1991. 001235 1 Last changed: 6/28/00. . 001236 Br EE ER ; . : BEST COPY AVAILABLE ) . Study Title ea Desante,Dupbmaerions| 73 2: 3 ffrAruetohsohr--se--n ERE EY uy, 19953 R LiE a CE e a eeus nante pacorpenites onot : b BN RSE RR m Sl S s y no El Sbarn Ea Ra lL 4 ER ie Tiare 130.5 Pa 3 Erna 0sI hd Ed .~ 001237 1. ma . freo de huecesdcoteH ,ln t4oni8cityL0iothfe Goma or TsTiCee-peH1r3T,atent2aoTatopeht9ent0,and1tuehrvaiaTdat,pchaeobnDidBazuvepc.iat"rdeeodsfsy1ou%trresant3e.sK ite Romebabs, Hien Seaatord, Robert SDiovniisciotnhoefPacerheurvceere:Bint comenetest maapuctorscewiheern s&tabileicnofsttiivetariteoendscsownuithrre t2eitv4de . -- tca TeoEmnpcemertarltautrolensaoCf1 oofS203EnL,CitPoTnR.sUetEenvacTdeadaaLitp0anaat0nriwi0eorh:enae:sr0asuMhmaosrvLaeNtoi4ntcncnaas)vteecacoeoptant.tcaal1tea0rpaTltmoeiarcnlenelds KomMallto i dente tons a oT ais wede 1a the tat waarpeiapirhdoedaucsehtdeadtfosfarnthsferoaacrtihaootneusveetMcetsueelrioah4rt BBortteaeeo deora e cotiteuioanuiatintghe1BoJsottzbnaigagofTbaee osftu6dy..03 91. : ne me tt sistance wEaastit1n,0t0o0troiacthoetFoteEsGtAumhTba.sitdusTzocestscieetstestlhtteeeLdd S=OBGEL i let as ohserved 13 tay tes *vessel dsiag te test. i Wootrm on ; ii ds Ah ba ec Ss Re gsi REL& os Eee .v A BN Ee oanS bbaeE a FA ata a & Sy : i a BEwi EH S C R t D aaE ene e ls eg i = ih Tg La BBERaLEe Be phi 3 al I SChR e PB " : 001238 . i & BEST COPY AVAILABLE 11 as or commer : stron: na 1. Susmary 2 fv. Tsetion s ) III. Index of Tables and Figures . te . 7 Sw Methods and Materials 5 Vii Ratiresces n LA Se ir Gaiters toel n icy ee 2 L ihnPE- SRRCE ER to SAih pl f lee ae e RO eRe IE pes SC Ha ur AES TE BA adN a l ae JU BEC Ed D 4 S oa RSa bh caeEde ERR Ld S[RSeaSEl i EPSAAT. pene PB PU : 7i gE EE AER Ena LE N ; BEST COPY AVAILABLE si ssn } ner wht. cmp pr of sp " - BE ) od iis Table 2. Survival data from toxicity test 9 note. ptesestnaan ocetations (550 w Table A.1. Sortoh Conductivity, pi, I SS temperature, and dissolved 13 G BEE a m a a l e fe Sn a Ec 4 La |=e E Laee s o i | oEERodT Eea ae abe e NEE a i feet eos I. es 3 Vi b SR I 001220 rc re 3 BEST COPY AVAILABLE mv. omen neDeseeeechisnits+toTty eavis]IsppMoeaTnvecseEroLLttaralebbedyrsLus1t3osespoCcroThmehepdaaanh1fytaoa,poveaurhSntts.taicospPxoapaivnuercalin,i,tdenysMdionmfdsCaeaeacttshtboeitoirsnce.csusmreutnTlhhttatestso } 8 Sm CT de tt v.. moa n mmss . esr sooner: CEorrsttoreneoat,our (EEEsvSTiTsBIritienns rSSiulUsiloTveisRbaaIinbfanorermdaItidTcasH: 1"0Sw.a9Ns%CSpdl,aasltTiiEmc-cAehNdoEtTt"it:eo -- Te B IT e tan ee rage eTbeier nn,Tsicikllaert)roovswosi.wiaeensaisephtpehaitdsodrt0(oamYpaTet3ooeusrriiCeaa:oalmtpevalasy.s13h.3o05reGdeBuumri)kn C3 sT cuounmaionnCawte eerrs:wSenid eiftoreosrceeceleitaoatbincofoahGsastB4ipoTuvrelipmgtreuoetntsiamsrtnaiestenanstsaindvteLaafossruBapaaaln1rstoeotnfo,xLiwtcaikHteeysv 5; Ee eh uit 1 ai wh are presented in Table 1. CogRE mer eeAEC von 0 muter iteE : istered us by wl tat orpuatonsvce tren stogte aT asT cultsiadTwoa a2 thes F B LE Lle e e n te oe E s I etI R enteereat e e elt aniitteea wienvee Pp p B B |rA n R L S m R eR e teneiiTaaBe i ealenrrtsr sTfihoadeadse0 OIEW p1 a EL eleeErS a e h te es Wt IR ERAR E SeSAR PON SC0 EsCboabC eilah a IT R a NMaERi a Te E EE a LG e Teeae l 3 Gite Ee Ga Pee i M - Ir B } vi 3 BEST COPY AVAILABLE Talal SCheal achunrcteritmtion d of a reed mstatiiea sale of } rL r ssa T Ea ate [A - condnetiviy --. - wo n Secosmtoicettiioeriae o; s cSmpeopteontorss wont 3 - rToilveartosriated wit 1 i i; : a eeEn T 31i,T erabcoaeltteeecetedafrCenoatnhoetSvneeiatttBoenwerbeaeoseesbobrnusttdT1paaeldotlsvot.ton ~e; FL eum RLTRNIRINGG + introduction of orgaanndipes stimcisde SIS and PCB WG data is. Sh oo aad lol 0 Rs Seg ERan LE : Se B e goioo a RE anlTaIe lIR l RR e i et a es ee FRI a , PHnSdESEeRD Ea a a EE er aE BERR i aes a A Lene el kd Pl ) } --- seater vasTone: m8ay 200hA eoscr1e0TImLaiwaaglhka,ntkes1t00 vN"oiamtadh/ekadthecsoantdceestn3t,rs0au0tb0isotnmassgc/eohfvatseArtctoasnrhtbiec4ttaednmcoedvuesrbiesrofge B GSTorrieanteor aat0th1e.rS0eoAw"aealskk',e 10a0 afasbni,tevai1v2a8da1a00at210010/00i000saSlwrlia8n2ag4odSfoBaOpNb48atSdhusravueisvxep)voesroefed tases. E1i3r9e1'. coTnhceoeentdrtaaettsiitnotnvtsa.isvoocfotnotxaiteccstitet.dy Staetusbtsatvetsahcseopue1s8tSdoecm4apaedgSsidtluaurrtsiinogsotwJ3au0tneer41C3TooCnt0rvoi1lt4:,h Ay 1S7TS,aero0rmit0ar0,ol)B1e5"h0ouRtsateural,chc3o5n"0sco3eln0utktri.aonti40o`0nvsaMsaorfflor4iah0ue0.ia0tt7eeadhtvi2mt0ah4toeur1ri1a0l00"tvhe0e.r1e:vLeeo sof/h4 -i CBeolpkleircsatTeesa`tshtayGtE cd4uoApnhEtahattdasnSscveesr3t0ea0.nrtusAsLtoTnol`obyf t1e`9ts4etstevqaussaoltlpuycttidooirnsmtecdi(deu1ahtieed2r50sdaeoalspgthGifeovsueers pfSe2rTcouixcbituucoartneetGyurTtocokgtiomnT.ha of48kTohesohtseve"esvsestsesle)l(.ss`FAwianr1de6o.nhoTSuaiarmdboeinrlsyb.taa1btlveenswoihesdosvuese1dbduro1kn5 . iTPhitohtoospreri"tohsfaatcsvapkrrroevasiaatsfcsoastxMiacianTitilenyltn mp"Siiinasktseucsiisovieetddy wocfitehs2c0ooclwoonEvcsbeiinRtterta.tliuorneshesrcaabetoinvoten TeCuptabie Towels. Divas vere aot ged ducins the Sort: SsaenicibattliI ionneioonf I o(tIhnearbilistuybleStohalerienffecWtisthin(lo1s5s. ofsecsoc-- nudisiibe"siiitme-- ,r ogFeraattiisc A HB1a5ib"miivinogr,]souLvroessr:se Di`donsftsecrrieavfileaeedxdc.rvigseeexncsilti(ay1b2it1laiRdtoydF,eulco5rf7idsecsaesttaoeirrtsaitsiIionnls,yicsoifansdcbtAafnseeeearbe2ir0n CoToanRauLttte)impvpe.irhtiytur(e`h(.aCcaklaa(sInSrTHaosdmteercsu`raDybHEerh1e3r. aRcmLae-etSte0err,s:tonsstttrrheooamreasonaattetnSeraaabbBeerar,bePPrAALL-ZCE3SL6D)5):) Hi : TTeicroe i"nIhattiuer.ed ad recorded SEAaLilneLhsYeh Et Chobe EBAY Contained B iiliBFe o nSHA t ERLL LiTRohae SM etG lterRhacoieta be fatersretet CCUEED. sGrueaantaeirioecactuartrieadtiac6al411tacehsatiaesdesc,om(eeupmbtirsastio19n8s3s) because S08 survival oF fo ody% copes TeB E dL e LT Vg sili =gi i CRE i SEE er eteseein de. T0IDs E L BARO R RAEE SrSD E AR s a oii N Pi 7 an 00124. ET Tm oH5. +90 } +" BEST COPY AVAILABLE 2 wins 3 d cGietedsJt bnpattesettdyieAssesehls c0 moInteinaa iagJFJ LCr-ItONsCI E wBereiE tSottT isaellaiyritecdlyeutsea1tt4yo4d } SEe0,5 EeeTrLieStCaattzormiStsoWL ILITILIS Serer he } mcFo"EmeIe"nfhetestershpt mpesuebsetan3cSI e was mot EtoPxnRirLc Satoof daa appnbiaiatdssseoattsewitdeesatnetddo Ahi it - BSlywe, cl S cfueE eniEnREgaRRE LSETF ;: Bae Fon al Ga n "rlEo oa 7 c Te k f nRE h Y 0 nptai B SEnRE BR Ce as P fo eh a SE SS aCE oe po I BObeElnoit7 idE e Ta ee A BN gh A " RN fo Bi get ey npg ory CDSE . BEST COPY AVAILABLE ; Table 3. Survival tata tron tontetty tent -_-- -- csoonxWctmzitasrien we TI TBwmaM buewbawtovI aww eeTr[ eweasa-- twmerdae Clem Co P 1oso 7 o sir s dos ds d ro s8o s e 8 N _H 10 1 P Pos o8odo3srsrs 3 s aoo8s 0 1 PPoossoof r38ssrs 0e 88se, wo 1peon0ohs Gaind so41sb oso 0roe08g 8 P SC h tE rR a wo ]AC RBRH ERER 5 sili Sapkio to nao. ERE RES Ls Teo baoGabgogbfa li aenBsosnakciSIE iRRR R rRs edeiEc l HSe plEoOee sTiiTYo0e |E To8 a i I S3 r E = fsocondarastesunt Atemclatole ns)estsi8 wTBitche oiq tntTst 1e57 AE BE i ES 4 CA 2 SaRR aay on JO I Li Lf TE i A |TTIARTE aeE EERE Rasa BEE ed ed PE RMAPEGi i SE RABER ST E COPY AVp AILABLE : Toles. Neen effective concentrations (BO) trom tanlety tart: weno conthimes Hates rer wre now ~ : 24 hours 71,000 ng/L - - ae at SA E IRR EC es 0 Ca Cb OR eTRE ges 3 Sisddha ) B G SSE UEE S 1 a SH DSLR e 2H c s oEi R abar Lat NT 3a ae he td I T001270 Fea Eh EERE TE HR i BEST COPY AVAILABLE i vr. amma i } 2 to B LedoSpre., aa19rM,.n,GeidSellioeles foT M r TestlA og of ChemicH als. Suction 3: Eftects on ) | ; % $3 1 go FE CL hl % Sih hf SeE TA)e RRhoe a s bl E ha a a e . | 5 MES oaSRfoTRfe EE SEAR oe SCENE as am |. J 1 Prosehi Pa 2:% EPaER a ge ES RGRR i ES R JE | |O0TZE7 SR Tn TEE, SE Th Sg i A B BEST COPY AVAILABLE pants A. ONATYphTonsonia ast TEETER Ee Jie Rak Lo " R ho alheo d uS BR e f TREe SR go (ESE ER: rEa Felis 3 els DRE CU ih PO BE t ih Tx | I E tLaam Nsd gel E ; J ) 3 -- 001248 BEST COPY AVAILABLE Table A.1. Cronotucmtiavitty,iBlf, atemRpeyratrurfe, tsaad dglassointad cryoss . --_-- conscoennitaration manrivity (sere) Tw ow arep5 eecCm l ew re M t en Lwe I F A lemon 1PPoodwwo w dwee mwmee 1o1m33y 1T13ys wwaodn leoWwoW mon dy . 0 PLPo ooEwow oMMwee ammeee Ww 111333 wwwoee saeaaa ae wl 0 1 PPo om me wmMeee mom mmmewe trai mon soowaann a sawaad om " : SLJioodmmee wMHmee oammmoo Aon Wed We 1a7s33 sawann es a3 da i BEEoFCheA ioeg. lAR10THISGriwee wmee ge a he aBwmRm BE 1TxseAeER nea hwes CE 18 Te Te SGiEpRhat Fane aS meERETRGe EER: GeopTOs A 1 no fol 1 sFeeai. in Eivio hain PFHoorreiT dacheO gsi EERGAEaoPa"r|arV4no0 BMSEeRae0Lr 1T3eR al io da R e CI U oRE eCRShETeE mO:borS oe R eDeRERR.. A d " y EBya Re oy he BR oa | E THoE batrs HER i foc # ' . _. - ! RT Tol AL Cotta 7 contseean=ttsion Co gTEToemeeErE PC0y SStiaREereeivted SoeTvrepneEo]s lem 1PPoonn1ds3 oBoaEBliioooBbmdar otBe oomw22 aiaa ond oBIOBI nH B - 150 LPo Poaos mene moBoasBEdDoOBBmsSa 1mmaoomo3da maes WoW 0 PPo om oBRi E OBBI o OW Y u mn W FS SO RS n 5 w E boooE bBs% B oBd iBoi RPPadPmO owPnYl V FR > EB 1 gBYhEa.rw BET "00 SodPRTE OBoBiSweEAaO wBRagEfeiR RisiSaeeR1eI3ssa3d3n aa dd BigEit en ea pb EEE 1 35, 2008 Cdslagagie ge 83 Ta 90a Tes eStBieali i an o LSeetts BFRanm aglEEel l nanEl83bee45a ad i Ey " a Ti GER CSR Pals | 1 LE Sais 9 pA ji! W 15 proL n fCaa Ln TTaa ap.Ee: |1 Te El e SEe RRE, . Pd TT . 00125