Document LJ1Mp2K4YkYz9dm6myMbG5G5w

321 BUSHY RUN RESEARCH CENTER ft. 0. 4, Mellon Road, Export, Pennsylvania 15612 Telephone (412) 127-1020 UCC BUSINESS CONFIDENTIAL: Not to bo released outaldo UCC without tho vrlttau consent of tho tlCC-sponsorlng Division HS&EA Managor. Project Koport 44-50 4 Pagoa Tel: (4X2) 327-1020 May 12, 1981 BAKEHTE Phenoxy Rea In PKHH Bangs Finding Toxicity Studies Sponsor; Union Carbide Corporation Peroral Intubation, rat - LD50 >2.0 g/kg; 1 *1 - 0.025 g in 0.25X agar plue 0.102 Tween 80. Pereutaneoua, rabbit - LD50 - 4.00 g/kg; 1 el 0.25 g In mineral oil. Uncovered Skin Irritation, rabbit - None from 25Z In mineral oil. Eye Injury, rabbit - Trace from solid; none from 25Z la mineral oil. Interpretation BAKELXTE Phenoxy Basin PKHH was no more than moderately toxic following single peroral Intubation and was moderately toxle following single percuta neous application, administration of a 25X suspension (poor) in mineral 11 resulted In no irritation to rabbit skin or eyes. Instillation of 40 mg per eye of the solid particulates resulted In trace corneal Injury In 2 of 5 eyes. .e Quantity: 1 lb Submitted By: Date Becslved: December 3, 1980 BBBC Sample Mb.: 43-379 UCC 063168 030 Report 44*50 Pag* 2 Peroral, Single Do** to Rat* LD50 2.0 g/kg; 1 ml 0.02S g suspension In 0.25Z agar plu* 0.10Z Tween 80. Condition* - Standard. S* attached peg* of atandard teat proeedurea. The au*p*nalon In agar and Tween 80 waa th* moat aultabl* fore to complete the doaing and 2.0 g/kg waa th* highest attainable doaag*. Dosage; Dead/ Day* to g/kg Dosed Death Weight Change, g Sign* 2.0 6/6 83 to 125 Yellov-brovn discharge from genital area (mean-103) at 40 min. Groaa Pathology - Nothing remarkable. Condualon* - No more than aoderately toxic following acut* peroral intubation. Percutaneon*, Single Doae to Rabbit* LD50 * 4.00 (1.12 to 14.3) g/kg; 1 ml * 0.25 g auapenalon (poor) in mineral oil. Condition* - Standard. Doaed under polyethylene sheeting. Dosage; Dead/ Days to Weight g/kg Dosed Death Change, g Skin Irritation Signs 4.6 i/4 1,1 -14 and 318 (mean*152) m 2.0 0/4 -106 to 212 (mean-64) Cross Pathology - La victims, lung* red; livers mottled red and light red. In survivors, nothing remarkable. Conclusion* - Moderately toxic following acute covered percutaneous application. ucc 063169 Report 44-50 Fag* 3 Skin Irritation, Rebblt. Uncovered Condition* - Standard. Applied in nlaaral oil. Raaulta - No Irritation on 3 rabbit* fro* a 23X auapanalon (poor) in alnaral oil. 031 ;< Eye Irritation. Rabbit Condition* - Standard. Instilled as a solid or In slneral oil. Results - Trace corneal fluorescein staining (indicating epithelial denudation) in 2 of 5 eyes froa 40 ag per eye of the solid particulates; no injury in 5 eyes froa 0.5 al per eye of a 251 suspension (poor) in mineral oil. h \ UCC 063170 i 332 Report 44-50 Page 4 Reviewed and Approved by; Acknowledgement*; Single Peroral Taata Percutaneous, Skin and Eye Irritation Tests Typed; WPC/1143-2 Roy C. Myejfs, &.S Study Oiraetor L. R. DePass, Ph.D. Manager, Oral/Dermal Department Frad R. Frank, Ph.D. Dlrsctor Linda R. Barnhart Master Technologist Kaaml I. Condra, B.S. Scientist UCC Standard Test Procedures 333 In all tests, Che nonfattad animals arm maintained on appropriate dlaca and water td lib axeapt during parloda of manipulation r confinement. Dosage levels dlffar bp a~factor of 2 In a geometric aarlaa. LD50a or LCS0ay along with tba 95X eonfldanca limits, ara caleulatad by tba moving avaraga method baaad on a 14-day obaarvatlon period. Weight changaa for survivors wara dataralnad at 14 daya following doalng* Toxicity Terminology for Paroral and 24-Hr Dermal LD50t (A); Inhalation 4-Hr LC50 (B) , A MU B, ppm Extremely low order ^ > 15 > 100M Highly Bjr PP* lio-lM Slightly 5-15 10M-100H Seriously 0.001-0.05 10-100 Moderately 0.5-5 1H-10K Dangerously < 0.001 < 10 Peroral. Compounda adainlatarad by stomach intubation to Wlstar-derived mala rata. 90-120 grama In weight and 3 to 4 weeks of aga. Percutaneous. Mala New Zealand White rabbits. 3 to 3 months of age. ara immobilized during the 24-hour contact period with the compound retained under impervious sheeting on the dipped. Intact skin of the trunk. Thereafter, excess fluid is removed to prevent ingestion. Maxima dosaga that can be retained is 16 al/kg. . Inhalation. Procedure A. Substantially saturated vapor is generated in a gas washing bottle by passing dried air at 2.3 llters/mln through a fritted glass disc Immersed to a depth of at least 1.3 lnchas in the chemical. This air is delivered to rats in a 9-liter glass exposure chamber. Mean vapor concentration is calculated from the loss in weight of the liquid or estimated from the vapor pressure at th actual temperature of the chemical during aeration. Procedure B. Substantially saturated vapor is prepared by spreading 100 gm or more of chemical over 200 cm^ area on shallow tray placed near the top of a 120-liter plexlglaa chamber which is then sealed for at least 16 hours while an intermittently operated fan agitates the Internal chamber atmosphere. Hats are then introduced in a gasketed drawer-type cage designed and operated to minimize vapor loss. Procedure C. Mist, vapor and any oxidation or decomposition products of the chemical held at 170*C are generated and delivered as in A. Procedure 0. Vapor at metered concentration, not verified analytically, is generated by feeding the liquid at a constant rate down the Inside of a spirally corrugated surface of a minimally heated one-inch Pyrex tube, through which metered air is passed. Resultant vapor Is delivered as in A. Procedure E. Aerosols are produced when solutions or suspensions ara atomized in a glass VAF0NEFRIN nebulizer using dried compressed air at 9 llters/mln (corrected) and 22 pel. The resultant aerosol of droplets averaging 2 microns In diameter is conducted directly into a 60-liter cubic glass chamber containing rata. Mean aerosol concentration is calculated from the amount of material atomized. Procedure F. Dust clouds are generated by a baffled Wright Duse Feed through which air is passed at 14 lltars/min (uncorrected) at 3 pel. The dust la delivered directly to a 12Q-litar plexlglaa chamber containing rats. Airborne dust concentra tions ara measured gravimatrlcally every half hour. Skin Irritation. The chemical la applied in 0.01 ml amounts to dipped. uncovered Intact skin of 3 rabbit bellies either undiluted or In progressive dilutions of 10. 1. 0.1. or 0.01Z In solvent. One of 10 grades is assigned based on appearance of moderate or marked capillary injection, erythema, edema or necrosis within 24 hours. So Injury from undiluted Grade 1. Eye Irritation. Eyes not staining with 3X fluorescein in 20 seconds contact are accepted. Single instillation of 0.003. 0.02. 0.10 or 0.5 ml undiluted or of 0.5 ml of 40, 13, 5 or IX dilutions arc made Into tba conjunctival sac of 3 rabbits. The yes are read Immediately unstained and after fluorescein at 24 hours, with one of tea grades assigned. Trace or no injury fr a 0.5 ml undiluted - Grade 1. ucc 063172