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BACK TO MAIN TOXICITY TO AQUATIC INVERTABRATES (Artemia sp) TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder of uncharacterized purity. METHOD Method: Draft International Standards Organization (Vanhaecke and Persoone, 1981) Type: Acute static GLP: No Year completed: 1985 Species: Artemia sp. Artemia source: Salt lake Brine Shrimp Inc. Artemia age at test initiation: naupuli < 24 hours old. Exposure period: 48-hours Analytical monitoring: Dissolved oxygen, pH, conductivity Statistical methods: Not noted. Element values were calculated for each replicate series, but not combined for the whole study. A cumulative mortality-concentration plot was used to estimate the LC50. Test conditions: Dilution water: 30 parts per thousand NaCl solution Dilution water chemistry (initial): pH: 8.0 - 8.2 D.O.: > 6 mg/L Stock solution preparation: 1000 mg/L; noted as cloudy Exposure vessels: Not noted; solution volume 10 mL Number of replicates: 3 Number of organisms/replicate: 10 Number of concentrations: 6 plus a blank control Water chemistry during the study: Dissolved oxygen ranges (test and control): > 6.0 mg/L pH (test and control) 8.0 - 8.2 Test temperature range (0 - 48 hours) 21 - 21C Element basis: mortality BACK TO MAIN RESULTS Nominal concentrations: 1, 2, 3, 5, 10, 20 mg/L Element values (95% confidence interval) calculated per replicate: 48-hour EC50 = 9.4 (7.4 - 12.1) mg/L 48-hour EC50 = 9.4 (7.3 - 12.2) mg/L 48-hour EC50 = 8.9 (6.7 - 11.9) mg/L Mortality of controls: None DATA QUALITY Reliability: Klimisch ranking = 2. This study satisfied all criteria for quality testing at the time performed, but actual concentrations were not measured. Results were based on nominal concentrations. Additionally, sample purity was not adequately characterized. REFERENCES This study was conducted by Beak Consultants Limited, Mississauga, Ontario, Canada for Panarctic Oils Ltd, Calgary, Alberta, Canada. OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 6/12/01