Document LBQ89GrprOonjZwoRj0L7JR3

Katherine E. Reed, Ph.D. S;aft' Vice President Certified Mail 3M Environmental, Health and Safety Operations 900 Bush Avenue. Buildinp4^-2E-26 PO Box 33331 ~ St. Paul. MN 55133-3331 651 778 4331 :' , r n ge*K>- -00373 . 73 July 28, 2006 Document Processing Center EPA East - Room 6428 Attn: Section 8(e) Office of Pollution Prevention and Toxics US EPA 1200 Pennsylvania Avenue NW Washington DC 20460-0001 Co HTa iN N Cb i Contains No cm RE: TSCA 8(E) SUBSTANTIAL RISK NOTICE ON: PFHS and PFOS; Supplemental to Sulfonate-based Fluorochemicals, Docket Nos. 8EHQ-1180-374; 8EHQ-0381-0394; 8EHQ0598-373 Dear Sir/Madam: Preliminary data from an on-going study sponsored by 3M that is being conducted by TNO Laboratories in Leiden, Netherlands suggest an effect of perfluorooctanesulfonate (PFOS) and perfluorohexanesulfonate (PFHS) on body weight, food consumption, and serum cholesterol in 15% dietary fat fed E3 Leiden transgenic mice in vivo. Cholesterol was reduced at dietary concentrations of 30 and 100 ppm for PFOS and 60 and 200 ppm for , PFHS. Food consumption and body weight were reduced at dietary concentrations of 100 ppm PFOS and 200 ppm PFHS. On removal of the 100 ppm PFOS dosage-group mice from exposure through diet, food consumption and serum cholesterol showed recovery, but bodyweights remained low. On removal of the 200 ppm PFHS dosage-group mice from exposure through diet, body weight and food consumption showed recovery, but serum cholesterol remained low. The lowered serum cholesterol in the 200 ppm PFHA dosage-group mice is being observed for recovering in the on-going study. 3M believes that this is the first observation in mice of cholesterol reduction as a result of treatment with PFOS and PFHS, and the first observations in mice treated with PFHS of reductions in body-weight and food consumption. PFOS and PFHS has previously been shown to have the effect of reducing body-weight and serum cholesterol in rats, and PFOS has been shown to produce the latter effects in monkeys. Therefore, 3M believes this information to be corroborative. A final report will be forwarded to the EPA when it is received from the test facility. If there are questions, please contact Dayna Blomquist (651-736-5413). Sincerely, 89060000402 Katherine E. Reed Staff Vice President, Environmental Health and Safety Operations 0000