Document Kzyyd4rOnqydQevDxGXKYvBMw

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorooctanoiaccid;may alsobe referredtoas PFOA, FC- 26, or FX-1001. (Octanoicacid,pentadecafluoro-,CAS # 33567-1) Remarks: The 3M productiolnotnumber was notnoted. The testsample isFX-1001. It'psuritywas not sufficientclhyaracterized, though currentinformationindicatesitisa mixtureof95 -98% test substance and 1 - 5% perfluorochemicailnerts. METHOD: Method: Not noted. Type: Staticacute GLP: No Year completed: 1985 Species: Pimephalespromelas Supplier:Dale FattigofBrady,Nebraska. Analyticalmonitoring:Temperature,pH and DO Exposure period:96-hours Statisticamlethods: Probitanalysis Test fishage: Not noted. Length and weight: Average length 4.1cm Average weight 0.50 g Pretreatment: 25.0 mg/L tetracyclinHeCI,5 months initiation. priorto study Test conditions: Dilutionwater: Carbon-filterewdellwater. Dilutionwater chemistry at testinitiation: Temperature: 190C Dissolved Oxygen: 9.5ppm pH: 7.8 Stock and testsolutionpreparation:A stocksolutionwas prepared by dissolvin4g5 g testsubstance(neutralizewdithNAOH to pH 7.5)in3 liters ofwater and transferrinagppropriatealiquotstotestvesselsto make 5 literosftestsolutionatthe selectedtestconcentrations. Concentrations dosing rate:Once Stabilityofthe testchemical solutions:Not noted Exposure vessels: 24 cm insidediameterand 26 cm deep glass beakers containing5 litertsestsolution. Number of replicates:two Number of fishper replicate:5 Loadingrate0:.50g/L Numberofconcentratiofnisv:pelusa blanckontrol Waterchemistrdyurintghestudy: Dissolveodxygenrange(24- 96hours): 7.3- 7.7mg/L(contreoxlposure) 6.1- 6.7mg/L (870mg/L exposure)* pH range (24- 96 hours): 7.5- 7.7 (controelxposure) 7.5- 7.5 (870mg/L exposure)* 870 mg/L testgroup (secondhighestconcentrationd)atagiven because totalmortalitoyccurredinthehighesttestconcentratiobny 48 hours. Test temperature (24- 96 hours):19 -200C RESULTS Nominal concentrations: Bk control6,90, 750, 810, 870, and 930 mg/L Element value: 96-hourLC50 = 843 (811 - 878) mg/L Element valuebased on nominalconcentrations CONCLUSIONS The testsample 96-hourLC50forfatheadminnow was determinedto be 843 mg/L witha 95% ConfidenceIntervaolf811 to878 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O.Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking2. Testingmeets thecriterifaorquality testing.However, sample puritwyas notproperlycharacterizeadnd it lacksanalyticaclonfirmatioonftestsubstanceconcentrations. REFERENCES Thisstudywas conductedby the3M Company, Environmental LaboratoryS,t.Paul,MN, Lab Request Number C1006, completed on February2, 1985. OTHER Lastchanged: 5/24/00 CHEMICAL CONCENTRATION (mg/1) CONTROL Cl) ENVIRONKENTAL LABORATORY TABLE 1. SUM14ARY OF ACUTE TOXICITY TO FATHEAD MINNOW UNDER OF t;klMl t EXPOSURE CONDITIONS 24-HR. PERCENT MORTALITY 48-HR. 72-HR. C> C) 0 C) 0 -(40 C) 0 0 1-50 4-C 1CIC) )4 c IC)C) tlc50 (9m5g2/lCOWIDENCE ILIMITS)>(2c)ty:)o a4q (f3 e>tP)@ %Ll) (1) Data were averages of -two replications for each concentration with t" fish per teK replicate. A total of Owe-t.-7- fish were used per concentration. .1@2 - (2) LC so values were calculated using logistic regression sethods (Problt Analysis). C-3) +kf- do5e/yespoKse- 8'10 I E, j., ti:,,r@rE iiF ::Ik.[,E LR --: OIGKtfA- rype TarbAZ20azr-@/,@, @lai:e-ri7,rizl--cit.-A3d rest ()rgmimk&gedfz@@s=-coi kvg.Wt.a, jayram Avg. Sizeo CIDNo Orgamsm 1)@at@R6ectdgA-:7 tz Batch - L4!@1Y) per dos inc3nctor-42Ll5L)- g/i PishDint%nnTreatment: rest soiuticfflvolumel.@- riZ@Ts@/All@-Glws Tarik Tank Dinwwims: w-4Ct, alld &4m-dew rimaStarted __@@Date Started Date Cmpleted Dilution WbLterCarbon-filtered well water (Tenip. Cop DoO.. @Z,-5-ppm, @H Lwest iga (FfltuulSU,lm (95% Cbnfidenae Limits) TW* Initial 7btal 24 Hours 48 Hours 72 Ho= 96 Hours th. Cow. mg/l Amount 7btal No. pH D.O. Thtal No. pH D.O. Total No. pH D.O. 7btal No. pH D.O. Added Dead- ppm Dead lppn Dead ppn Dead PPM Cbntrol A@ t@) I o@@49M0* 0 71 Test Temp. OC Initials & Date r@2C, ellq_ Tank No. Symptoms or Reactims )Dntrol 24-Hr. 'e50@ Total Mxdxw 48-Hr. Attectea 72-Hr. .42 96-Hr. r4i AV--'AT-C 'A"OXICITY DATA SHEE- DE*FIN:-.TIVE (FULL-SCALE TEST) LR V,V %If t4 tt5 Wpe TeSt@2@@@@@Materii3L.1 Tested 0 lot Test organi hvg.Wt. gram Avg. Fish Disease Treatment: Test Solution VolUme, ,e6L)5 Sourpp-2 Date Recld Size cm No. _qMSsm L-IGZd.ose L.:@@_"ding EWWR -77 azz -7 Lit=S/All-Glass Tark TarLkDimnions- Z14 OLXI) - aw R;!g un @4@L Batch .1 i;e -9/1 Tim Started Z@Datig Started @2 Zn- DateC=pleted 1,-z2als: Dilution Water-Cikrbm-filteredwell water (T@mV. <----- - - I C., D.O. Irwestig (Ipftnu s (95% oonf idence IC50 l3Wj, @H Tw* No. Ir Initial 7btal Coric. Ammt mg/l Added CcD)ntro 24 Hours Thtal bnbo. Dead 48 Hours D.O. Thtal No. pH ppm Dead 72 Hours 96 Hours D.O. !rbtal No. pH D.O. T:bo)tztaNlMbop.H D.O. an Dead ppn Dead ppn Test T?w. OC Initials Date /e-7 Tank No. bntral Symptcm or Reacticns 24-Hr. TC)tai MXUDer Atfected 48-Hr. 72-fir. 2; - 2--2- 96-Hr. r r-..