Document KzR6rL9dEjqwDdLkOjVmg5bxr

133 CLIFF CAVE ROAD Younger Laboratories Saint Louis* Mo., 63ia9 HiochemUti . . . Pkcft*HCtfftloyi6i4* .,. iQtuzltydid 1 { PBONEi TILDEN 6*2540 Cetiijficcde o AmiifiH November 12th, 1964 SUBJECT - Toxloologloal Investigation Oft MCS 512 Monsanto Saapl* Nusber 146 Monsanto Prolast Hunter T-64-133 8TOBT CONBBCTSD FOB Monsanto Cospsny, st. Louts, Missouri sxjPrihMTAl paocmras 4) Oral LDjo (Bats, Mixed The undiluted coapound sola and faasl* * k'___ I _____ by aiyifo^^ub* to apragu*-0*wl*y strain albino II II. 1 ms datarsinad, groups of sala and at inoranants of 0>1 fractional log blanks! tha toxicity rang* thereby alios of tha LD,0 ubieh vaa don* according to a T of j. da boar, Obaarratlono wara'ssder'for toxic ayaptoas and th* risowa of th* anlaals that suosuabad *r* axaninsd mcroscopically. Tha data art shorn in Tstt.* 1, B) akin Absorption MLD (Babbits, Mix*d Sax) Th* undllutad eoapouad ms applied in lnoraasing doses at lnaraaant* of 0,2 fractional log Internal* to th* closely slipped, lataot akin of M*a Zealand hit* u*l* and faaal* rabbits, Th* treated araas sera covered with plastic strips and th* anlaals plaosd in soodm stocks for periods up to tssntyfour hours, attar which tim they oar* assigned to individual cagaa, Obsarmtlona were sad* for toxic ayaptoas and the viscera of th* aniasls that suoouabsd were ersalned sacroecoplc&lly. Tha data are shorn in Table II. 0620410 Thi, confident!,! npott i not to be nwd in any ioim cl advcitinn, without wiittcn psrmttuau TOXSTUDIES0678 ioi Monsanto coapaap 3t. Louis, Missouri Younger Laboratories Certificate of Aaclpsls - Page 2 (11/12/64) - 1-64-133 tXPSRIMJITAL PS0CE3URS - (Continued) C) skin Irritation (Rabblta) Bw undiluted compound aa applied to tbo clipped, Intact akin of albino rabblta and removed after twentp-four hours. The application saa covered clth plaatlc atrlpa to retard evaporation and arald eoa tealoatIon. Obeervatlone aero aadc ever a period of eeveral dapa far Irritation. Tbo data, aeorad according to the aethod of Dralae, soodard and edverp (Journal of Phan, and up. Therapeutics, voluae 62, Daecaber, 1944) are chant In Table III. D) SJre Irritation (Rabbits) 0.1 Milliliter of undiluted oaaplo vac placed In the conjunctival aaa of the right ape of each of three albino rabblta and obeervatloao node over a period of eeveral dope for lafl enaction. Ihe epoo earn rlnced nth vane laatenle oallne aolutlaa after tooatp-four houra. Bio data, ocored according to the aethod of Dralae, at al, are ahavn in Table IV. S) Vapor Ichaintlon (Hole ante) Pour 130-grua anle rata acre placed la a glaaa doclceator, 230 aa In dlcaeter, and expound far alx houra to a eonoentratad ataoophere of vapors produced bp passing a atreaa of air through loo alllllltere of the coapeuad contained la a 250-milliliter grlecmeper flack. Vapors from the flack passed into a aae-lltor bottle to reseve droplets sad then Into the ehaaber. Air floe through the anapie one five liters per alaute aa aeasured bp a calibrated rotescter. This sms sufficient to vielentlp agitata the liquid. No suppleseotarp air saa Introduced inasmuch as the above supplp aaa aaple for the aalaala expgea requirements. The animala cere observed for behavior and, elaee there sere no deaths, all cere held for tea daps observation. Ihe data are shorn la Table v. 3UMMARY Mg|__?lj A) Oral LD,0 (ante, Mixed gee) The oral 1D10 fer sale sad female rata saa placed at 6500 sllligrsas per kilogram elth loser and upper Halts of 546o to 7733 nllllgrana per kilogram. The eospouad saa elasaei as praeticallp com-toxic bp oral ingestion la ads and fascia ruts. B) Shin Absorption hld (Rabblta, Nixed Bex) The Minimum Lethal Dose bp Dkin Absorption la ada and fasala rabbits nae found to be greater than 2000 milligrams per kilogram and leas than )l6o milligrams per kllegrnm. Ihe compound saa classed aa sllghtlp toxic bp ktla abaorptloa in ado and faaale rabbits. Cb20bll TOXSTUDIES0679 Mt Hanaaata Caa/aa/ St- Mala. Mlaaaarl Tatagar Laka/atartaa CartUlaata *t AMljraU Mga 5 (UAV<4) - T-44-1J) anaurr - (qmmiwI) C) (kl> IrrlUUM (Bakklta) tu Majmai aa alMaai u * aUgkt afcU lrritaat Aaa sprUai ottliM ta lstMt vakktt akla. Xka araraga kill-- aaara aa* 1-3 aat of a jaaalkla la t*aat/faar haart. B) ora Xxrltatlaa (koktita) Ika aaa/aaal aaa alaaaal aa a aUgkt a/a lnitaat. Ika araraga aarina aaara aaa 1)4 aat af a gaaattla UO la taaatj^faar kaara. I) vagar lakalatlaa (nala lata) til aalaala aarrlral tka ala haar aa/atma aa aall aa tka (allaalag taa lag afeaartatlaa /arl*4. It aaa aaaalalat tkat tka ra/ara aara graatlaallg tan-taut a aalar aaallUaaa t tka tast> rcwour LAaoitTOHM 7^''-- I f'" 1 m ru m looma The ijiDterial in this report is to he used in development of the product and may he given to responsible sales contacts, but it is rot to be used by them in edverti/ring cc.py. The tiovuxeo.'t ` ' ' `p ' i1 h ros \'o he divv'fie.l nr.t.h :i; appears in formrl f j v i ^-n No exc ->;.om to tl.'jc-tab1,<3,:.''d rule may be ri;:---'vt.;hcijf, ;.:se acp; c"b1 of ih-i tV.edk.Vi Depart* XDent iu J-jc, /.ct.-iS.t. .-n ors' mjm -r:es v'-f.irdir;>T mutters of toxicity tv <. > L * ao .> go'c to to the Medical Depart* iBMt in St. Louis lor reply- " Monsanto Chemical Company 0620412 TOXSTUDIES0680 Tot Koaaaata Caapaap At. Lattla, JOaMurl Taaagar Lakaiatarlaa Corttflooto ot Aaalpaia - Poe* 4 (11/12/24) Y-64-155 imi i THI MU. 10,* or 'MM 51* ' 70* MM Aaapla 7o4 Dadllatat Asiaal *? - St* Wtifkt o. 1- raaala 2* raaala 5- Mala 4- Mala 5- raaala 6- raaala 7- Mala *- Mala 9- raaala 10* raaala 11- Mala 1J- Mala 1J* raaala 14- raaala 19- Mala It- Mala 17- raaala It- raaala 19- Mala 10- Mala 199 m 2JO 229 220 210 240 2J0 8 290 2J0 *19 229 249 2J0 210 229 240 290 Dm* to./i*. 9,010 9.010 9.010 9.010 9.010 9,910 9,910 9,910 9,910 2,910 7.940 7,940 7,940 7,940 7,940 10,000 10,000 10,000 10,000 10,000 .........r>ta BvriraA 0ia4 Mnlrat iwvinl Sarrlrad Mat ter*ir*4 SartrlTrt Mat Sarairat SarrlTag Mat 0ia4 Dla4 Sardra4 Mat Sla4 DIM DIM Diai dikjwko* - Tka OtoI um tr oolo aa* till iota aaa Plata* * ijoo allllgraaa par kllagrta Ilk lottr Mi mw IbUa * 9440 to 7799 mm grata par kllagraa. Tht nwiwt taw alaaaa* a* ppaattaallp ata-taala kgr oral lagaatlaa ia aala aa4 ftaalo nla. Iintnl ktaa oaa aaa ta (w *aya aitk ml daatka oooanlag ia ta tapa. Taxi* tpaptaaa iaaladad aarara ilarrkaa, kroaora, gpapait, aad aaXlapaa. it aatapap tkaro aaa laflaaaatiaa af tka patrla aaaaaa aai raaal hppariata aatraaaapiaallp. 0620413 TOXSTUDIES0681 Ml MUUtl 0*ap*V st. Uaia, KUMurl Xaaafar UkwtoilH OartUlMt* *f Aaalpala - P** 5 (UWM) - X-44-155 imi n TU NXnuni LSWA1 DOSS or 'SCO }11' it skid usoamo n sabxh SMpl* AppllM Badilata* Aalaal So. Saa MiCkt * DM* /* (alfkt Char** 5 0*r* UMr K>. 1 - Hala 2.7 794 I - raaal* 5.1 ia<o 5 - Hal* .1 >000 4 - r*Ml* *7 510 } - Ml* 2.6 5010 6 - faaala t.s 7940 o.i - 0*1 - 0.5 - 0.4 - 0.4 -- Ifcta Sunrlrad SarrlT4 SarrlraA 01*4 " ( Day* Mai - ( Dap* Dl*4 -- 4 s*r* discussion - Tk* HialM* Lathal DM* hr Ski* AhMrptlM la Ml* **4 Taaal* mhhlt* m* tmuai t* h* fnatar thaa 2000 atlHsraM f*r kUipM a*4 laa* tha* 5160 alllifnut par kil*r*a. Tk* OMpMkS *M *la***4 a* alishtlp taal* hj akla akaaptUi 1* Ml* ui faaal* rahhlta. Sarrlral ilM m* tmr t* *1* laya. M*U ajmpiM* iatlaM graMl 1m* *X appatlt*, lMraaaia* aaakaaaa, aaS tnaan. TlalMt nmUMw nt aoaaUa* Mr* mM apprMiMtalr taaatj-faur haw* hafara tMtks ' At Ut*p*r than Mr* hMorfhasU araaa 1* tha lua*a a* a*u a* Urar Aiaaalaratloa aa4 raaal aaa(*atlaa MaraMapioally. Obi*"'' TOXSTUDIES0682 mi Honsaata coupon/ St- Louis, Missouri Touafar Lakoratarlss cartlfloaSo of Analjrala - Po/a ( (11/12/64) - f-64-155 TABLE IU SUM IRRITATION RABBITS ARTER APPLICATION OF 'MCS >12< Saapla Appliai OaillutaS Aalaal Huahar Nsaarlaal Eraluattoa At TBa JM Of 1 Hour 24 Beur; 4S Hours 72 Hours 120 Hours 1 2 5 ATmfo 0110 02 11 0100 o-0 1-J o.t O.J 0 0 0 0.0 DISCUSSION - TBs oospsuaS ns slassoS as a allB Mis lrrlUat afcoa applloS uadilutaH ta intoot rakklt akin. Iks aTStags aaxlaua soars aaa 1-J out of a jpssslkla S la tra^lmr Basra. No skla oBaasas wars aataS la aaa Hour. oramlNkt tksrs oas haraljr parcaptlbla raSaaas oa too aalaals aad soll-AaflnoS roAaoao aa tBs this* aalaal for aa ararat* soora of 1-)- No odaaa AoralspaS- HtBla SOTout3-tie hours two aulaala uara fraa of solar aaS all raoalTad a soar* of oars la firs days- 0620415 TOXSTUDIES0683 Toi Monsanto coajaay 3t. L6ttl, HUMlirl laaagar Labaaatarlas Cart!float# of Analysis - pact 7 (11/U/M) 1*64-133 11ILI IV m IMITATION IX HAMITS AfTE.ll APPLICATION Of `HCX 312' Saapla (0.1 KUUIltw) Appliod Undllatad Aalaal xuahar Nuaarlaal Eralaation At Tho jad of 1 Hour 24 Hours 46 worn 72 Boars 12o Xaura 1 12 a 4 2 0 2 15 ii 6 4 0 3 12 a 4 2 0 13.0 9.0 4.6 2.a 0*0 DISCUSSION - Tbi aoapaaad aaa nlaaaad aa a alight n/a Irritant. Tha aaaraga --xlaya aaara aaa 13>0 aat af a pasaihlo 110 la tvatjrfawr hoara. Dlaaoafort aaa alld laasdUtalf fallanlag appLUatiaa. Slight radasss af tha palpahral eoajunatlvaai alight diaaharga, a traaa af adaaa, and vary all! Barnaul dallnass alth aa irltla doralopad althla aaa Hour. arytHaan raduaad la tsaatp>faar haura aad diaaharga aoanod. within naronty-to* haura ooraaal alarlty aaa aaraal aad all aalaala raaalaad a aaara af sara la flra days. 0620416 TOXSTUDIES0684 Tot Moaaaato CoBp*ny St. ioula, Miaaourl lounger Labor*tori** Cortlfloat* of Analysis - Pa*e 8 (11/12/64) * T-64-155 TABLE INHALATICM OT 'IKE 512' VAPOBS Bf BATS An|i reaperstur* laald* Chaster.................................... ............ Averafe Kslativ* Busidity Iaside Chester .................................. Aaouat Of Saapl* -- T Start ...................... .. 100.0 oc mmmt .................... 99*5 u Vaporia** 78* 762 * Aalaal No. - Sea 1 - Mai* 2 Mai* 5 - Mai* 4 - Mai* nte Surrlred Surrired surrlrad $wvinl Obumtloas Darin* Exposure No oMfcl diwharg* No dyapooa ... Hlld I*th*r(y ... No Makaoaa ar diaooeifort ... Aolaala appear uaaffoetod. DISCUSSION - All aaiaal* aurriTed th* six hour Bjwiuri a* Mil aa th* folloelog taa day obaerration period. It aaa oonoludad that tha vapor* ra praetlsally bob-ton* uador eoadltton* of th* t**t. As MBtloood la th* tabl*, th* *all* appeared to h* uaa/faeta* By th* ala hour exposure. Dlaooafort mata (*!-- aad th* oaly ebaa** la bthatlar * Mild lathargy after approxiaataly oae hour. The taa day ohaertatloB period aa* aaoaaatfal. 0620*17 TOXSTUDIES0685