Document KzR3ap111O9OaLNaRKmk1zDj0

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST SUBSTANCE Identity:Perfluorooctanesulfonylamido(ethyl)acetmaatye;also be referredtoas PFOSAA, FC-1 28 or as the major component of FC-127, FC-129, FC-109, or FC-109-X. (Glycine,N-ethyl-N[(heptadecafluorooctyl)sulfonpyolt]a-s,sium salt,CAS # 2991 51-7) Remarks: The testsample isFC-109-X. Currentinformatioinndicates itisa mixtureof 19-23% testsubstance,61 % water,12% 2methoxymethylethoxypropanol,2% ethanol,and 2-6% C4-c7 fluoroalkyl carboxylatecompounds. Test sample was from 3M productionlot2. The followingsummary is abbreviated because the testsample isa mixture of the testsubstance in a waterlorgank solvent solution. Data may not accuratelyrelatetoxicityof the testsample with thatof the testsubstance. METHOD: Methods: OECD 209 Test type:Staticacute GLP: No Year Completed: 1987 Inoculum: Activatedsludge mixed liquorcollectedfrom the CityofSt. Paul,Minnesota Wastewater Treatment Facility. lnoculum mixed liquorsuspended solids:2.9 g/L lnoculum holdind: None - used the day itwas obtained Analyticalmonitoring: Dissolvedoxygen concentrations -Exposure period: 30-minutesand 3-hours Measurements: Dissolvedoxygen depletionrateafter30-minutes and 3hours ofexposure. RESULTS Nominal concentrations: 2 Blank controls,100, 180, 320, 560, 1000 mg/L Elementvalues: 30-minuteECw=>1000mg/L 3-hourECw = >1 000 mg/L (5.9% inhibitioant 1000 mg/L) Allelement values based on nominal concentrations. Controrlesponse:The largepsetrcendtifferenbceetweencontrol uptake rateswas 4.3%; thus thetwo controlswere within15% ofeach other. 'Referencesubstance results:3,5-dichlorophenoalta concentrationof 10 mg/L induced45% inhibitiionrespiratiornate.Thiswas withinthe normal range seen inthislaboratorwyiththisinoculum. Remarks: Testingwas conducted on the mixtureofthetestsubstanceas describedinthetestsubstance remarksfield.The valuereportedapplies tothatmixtureand notthetestsubstance. No attemptwas made to determinetheimpactofthepresence ofthe organicsolventsorwhat portionofthetoxicitcyan be contributetdothe Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-109-X 3-hourEC60 foractivatedsludgerespiratioinnhibitiwoans determinedto be >1000 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 .DATA QUALITY ReliabilityK:Jimischranking2. Testingmeets allaitedaforquality testingb,utlacksanalyticaclonfirmatioonftestsubstanceconcentrations. There isa lackofcharacterizatioofnthe testsample. Because a mixture was tested,care must be takenwhen interpretitnhge results. REFERENCES Thisstudywas conducted by the3M Company EnvironmentalLaboratory, St Paul,MN, Lab Request number E2113. OTHER Last changed: 5118/00 LK Date 7 Anal E.WIRONMENTAL IABORATORY ACTIVATED SLUDGE MIXED LIQUOR Tip4iTait viraIt.,V\ t SourceAl,@irLC,cin,,@g.'Tatiii)ifvi'@Pldlt A; Date Obtained C. Date Used Mixed Liquor Suspended Solids (IILSS) @/L Adjusltmeni g/L PH Th,'4%,t Co=ents- Test Substance Sample Description FO - 1,,q-X -,(4:1t4-@oLeiZZ)FC' ot4 Sample PHI?-, O%t cm:; 7 AdfustaiiitA'@."@ e- Comments Reference Substance 5AMP le Descriiiti Sawle pH Coments,<@ir- rL,: "Justment'@@ dit Test'Temperatur, ...... rem lame 8 PWO c-E c N. Sample Desaiption A. EnvironmentelLob - Work Shoot AC-%4vckac,k 1-@lleLe Lt!"( Lei r,iqIL #1@,tc,4 AIrt#a /sca 1,0. .I . lic,* + Lob Ro"oit No. Pap of Doti: Analyst: It V!ll f- + 2.q q T4 4 .@4 4 *, , N7 :7 I _JL K, rail Ll7 7 ti LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST 0209 RAW DATA Test Substance: 16q- X Source of Activated Sludge Mixed Liquor: tr:, Date Recei d: ft,- -7 Date Used: ':77, Analyst: 30 - MINUTE EXPOSURE',' DISSOLVED OXYGEti CONCE!,ITRATTOTISv! '-7-est Substance iimt-miriuiES TEM0F Concentration(mg/L) 0.5 1.0 2.0 3.0 4.0 i5.0 6.0 7.0 8.0 9.0 10.0 c CONTROL 1 7. Y 7. a t.,7 -.9,.7.7 c 70 7.;Z 7,C -L. -7L-=L c.c6 1-q1. AID CONTROL Il 7. Tel 3 HOUR EXPOSURE 'TesT Substance Concentration'(mg/L)0.5 CONTROL I - /00 74 7.6 i 1.0 DISSOLVED OXYGEII CONCENTRATIL)!ISmg/@ TIME-MINUTES 2.0 3.0 4.0 5.0 , .0--71..0 8.0 i 9.0 1 a, .!57- '5.t Li- 7.;A@ 3t 7- q -3 T'EMF lo.-D Cl ;o 3,-R 3,3@ 7-1 boi5.5,5.o 9-t@-;193.4b '@ONTROL 11 7. '.57'.11! pliof ActivatedSludge Mixed Liruor: pH of highest concentrationtested: T e!.tTemperature: Initial7. Initia7l. q.C)i3 Fiiiai Final7.9 12 2.3 ;o ,-CJ4140. Test Substance ONIRMR-MAL LABORATOTY AcrivATEDsu=E REspiRATroN INIIBITla4TEgr 0209 CALaiLATio.NOF PxsuLTs Lc# Z) 30 - %11.%ZffEXPOSME Test Substance Concentration (m_q/L) M-%IMOI- T' O.Vgen U,,3tak-Pcate R 0 - .5c, c, R Corrected(') t6 2ec c ..5cvc, @-c ci c- .5 *q Li .:k 7 c- ..;"c r. c-. 7 cl'-I LR 11 Date to- lo -S;7 Analyst 3 el 3 I Inhibitionin Respir3tion Rate(2) (X).%IROLrT C.,- 7 7 Percent Differencein Controls(3) EC,.0 (95% ConfidenceLimits)mg/L Test Sub@tanc@-'' Concentration(ng/L) 1 t) CAr) Oxygen Uptake Rate R 0Li q c. c,.,5/3 r@ 0 0 q'73 Percent Differencein Controls(.3) ]Ecsn (95% ConfidenceLimits)mg/l. RT.Obs (1)Temp. Correction= (mg 0 /L-min.) RC, 2 RS (2)% Inhibitionz x 100 Ic1 + RC 2) hbere: 2 R - Oxygen uptake rate (mg0,/L-min.), .T.obs - -campletcmp C' 1.047, x T,o@s 2eC C - Control R Corrected to 2(f c C.-5,9 I-1 iy L45@C. L4 L47'1 % Inhibiti?nin Respiration Rate (2) RC, RC (3)1 Difference- x ioo",."i* (RCi" RCt2N 2 The two control respirationrates should be within 1S%,.ofeach,other.@. rem rome-4 pwo Re@ertn,,#U,ut% ReA 1.@.Jiltr Lot 'e@egig :51or-@4<@uiv,,On Is. LobRwsmtNo. .2 IOL )I Erwironn-antaLlab - Work Shat pop Petpif4tiort L i-L 142 -c4-, o cl Arslyst: S4-R .1@7 - '@4ccV- liiiItL VIA 3 eketL. 1r tvtc .4A%) -rh 14 1 Al tr@- "M 4 Tem Ceid, sez&.1 15' IP& OX womr f@%6 "w F .27$1 4 FIftl rvA AArcbL41., T IAll rn ..-,+,. ..%,fY**c I-rk -2 11 p :ietk4ler illid /Ll- Z -r,7. -7 LR '2-tL-@ ENVIROWIFTAL LABORATORY. P6 OECD ACTIVATRD SLUDGE RESPIRATION INHIBITION TEST.@1209 RU DATA Test S@6sta'nce:- *3.A- e @ect,@te t@N@i a iic @e Source of Activated Sludge Mixed Liquor: -kit4 ri'@ Tfp&impnt P)Ai,t- gt P,,,l iAAJ Date Rec I ed Irj-2,t- Z Analyst: ti RIP "Le I Date Used: It)-.te3-,k7Z 30 - MINUTE EXPOSURE iest bubstance Concentration(mgll) 0.5 CONTROL 1 7.1( DISSOLVED OXYEti CGN@ENTRATIONS ./i TIME-MINUTES - I TE 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 19.0 1 10.0 c 7.;I- L--7 -7 S.a 1 .'7 7 -20 7.1 6-9-It-.q4-C)i I'@ 5' 3.C-4 cONTROLIL 17, t,.i.R 6. 9 3a 3 HOUR EXPOSURE bubsuance coicentration(mg/L). 0.5 1 1.0 D ISS*OLVED delf"OtNCENTRATTONS moll 2.0. 13.0 '1 io T114E-H NUTES.-,-".,. @5.0:@ I 6.0@t*7-.O"8I.0 1 9.0 1 10.0 TEcMo 7,0 .'g6 - 5 ;c 7.5 t) %@OtiTROL11 @@-pH of ActivatedSludge Mixed Liquor: pe. cc.-.centratitoensted: lest Temperature: :Intiia7l-@ Initial7. 26 @PI Test Wbsta= ACRIVATED sil IA (Y B RESPIRATION INIIBITICINTEST 1209 CALCUtArION Of RPMLTS P4? Cere,-i(c"Tt3yic(ty-4 Dat6 Analyst 30 MIN= EXPCSM Test Substance Comentration tmpJL) tywrgnl-t Oxygen Uptake Rate R 0 @.6 C, -c.C)- R Corrected(l) to ZT C 0. @>-Oocl -7Ll I I Inhibiiton in (2) Respiration Rate PercentDiffere=e in Controls(3) L 'ECSO(9St Confidence Limits)mg/L 1',1-tai I FxrKlqmr-- Test Substance Cbncentra:t'lon' comoil I Oxygen Uptakf,Rate R q S,' ci 4'7 9 Iliftufp,l ;I- 11 R Corrected(l) to z(f c I Inhibiti'an@ Respiration Rate(2) '5q7 Pei-6ent D*iffereme 'Cmt roIs 31 BC (951 confidence'Linitsim@g"/L 0 Ilv (1).TeV., Correctia6z 1r obs .(Ng 02/L-min. x RC (2)S.Inhibitionz X 100 AC RC@ Micre: FL Oxygen uptake rate (mg 2/L-min.l@ .:T@oobs .;4sample temp. co 1 7, x T,i)bps,2.i-f,C C - Control 0.5q73 -I-etuc (3) 1.Difference ac X The 'tw'o controI rM i' T should be within ISI,of each PIN 4r- s@@cte--i@*.@i@i c@,c, Plf-ep.,