Document KzDKNbOkwoLXvybb8YaX0eV7x
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ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
TEST SUBSTANCE________________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206A. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was cc 785-22. The test sample is FC-206A. Current information indicates it is a mixture of 0.92% PFOS, 18.75% diethylene glycol butyl ether, 75.44% water, 1.83% Hydroxy foamer, 1.75% sodium octyl sulfate, 1.2% poloxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:_________________________________________________________
Method: Not given.
Type: Acute static
GLP: No
Year completed: 1979
Species: Pimephales promelas
Supplier: Commercial supplier in Brady, NE
Analytical monitoring: Temperature, pH, and dissolved oxygen.
Exposure period: 96-hours
Statistical methods: LCso values calculated using probit computer program.
Test fish age: Juveniles
Length and weight:
Average length = 41 mm
Average weight = 0.64 g (wet)
Loading: 0.64 g/L
Pretreatment: None.
Test conditions:
Dilution water: Carbon filtered well water
Dilution water chemistry:
Hardness:
236 mg/L as CaC0 3
Alkalinity:
221 mg/L as C aC0 3
Conductivity: 430 pmhos/cm
Lighting: Not given. Stock and test solution preparation: The test material was added directly
to the dilution water on a weight / volume basis. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted.
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Exposure vessels: Glass tanks (36x21x24 cm) containing 16 L of test solution. Number of replicates: 2 Number of fish per replicate: 16 Number of concentrations: five plus a blank control Water chemistry during the study:
pH range (0-96 hours): 7.9 - 8.0 (control exposure) 7.6 - 8.0 (1350 mg/L exposure)
Temperature range (0-96 hours): 20 - 20 C Dissolved oxygen range (0-96 hours):
5 - 9 . 1 mg/L (control exposure) 0.9 - 9.1 mg/L (1350 mg/L exposure)
Remarks: Fish were surfacing in all exposure concentrations, except the control, throughout the study. The dissolved oxygen was below 60% saturation in the exposure concentrations by 24 hours, and remained low for the duration of the study.
R E S U L T S ____________________________________________________
Nominal concentrations: Blank control, 420, 560, 750, 1000, and 1350 mg/L.
Element values: 96-hour LC50 = 1303 (1094 - 1930) mg/L (Replicate 1) 96-hour LC50 = 1228 (1098 - 1485) mg/L (Replicate 2)
Element values based on nominal concentration.
Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C L U S IO N S __________________________________________________
The test sample 96-hour LC50 for fathead minnow was determined to be 1303 mg/L. with a 95% confidence interval of 1094 to 1930 mg/L for one replicate, and 1228 mg/L with a 95% confidence interval of 1098 to 1485 mg/L for the other replicate.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking 3. Testing lacks record of methodology used. There is a lack of characterization of the test sample and the study lacks
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analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 4970S, 1979. O T H E R ___________________________________________________________ Last changed: 6/26/00
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AQEUNAVTIRICONTMOEXNICTAITLYLADBAOTRAASTOHREYET
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Type Tost frE-Mtaf ^Vable. Material Tested T" (7^Z & L , A
..
Test O nianiaT*VW ^ rl
-----( MpA. Date Recd. 6 -7- 71
Batch No. <%
Avg. Wt. 6 4 . . 7 . grane Avg. S ite 4 A 0 4^ Ho. Orgaoisoa por doao 1C____________
Test S olution Volant IA L ite r /A ll-01aaa Talk Task Diaaoslons: 34x21 ca and 2A cat deep
Tiso S titto d ^ ^ J ^ i^ v j^ Onto S ta rte d
Data Coaalatnd
1 - 1<1
.4 D ilu tio n Water C a rb o n -filta ro d u e ll water dean. Z o C .. D.O. 4. pen, pH g.l )
r>; I(nFvuelsl tSigigantoarturYeV)y v i
1 * LC-- (95%Confidence L llt a ) f g o 3_ j y ^t v_______
"7V tnit. Talk Cone. No. _f/l
Control
U B 42.0
Z 60
s -7^0
Total"
|panama ----- iriHoura
-----a n ICS
oar0 h r t r u '
Anoint Total
D.O. Total
D.O. Toth!
1.0. Total J
Added <*.77>
No. Dead
pH
ppn
<5.9
0 7.6 s.s
Ho.Dead pH oon e S.o c.? 0 7.6
No.Dead at Ppn No.Dead !h
0 2i s:? O 76
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coMwnts:______________________ BEgT r n PV AVAILABLE
Effects other than M o rta lity CInog/ni1te.. Synptoas or Reactions C o n tro l
Signature $ Date Comments:
BC,.^
24-H r.
48 HTr.otal No, A72ff-eHcrt.ed
------ jS V H o va C.
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96-H r. .V * <V 1 s ________-
0 0 1 6'0 3 .'i
BNV1MNMBNTAL LABORATORY AQUATIC TOXICITY DATA SHEET
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Type Test 16-Uou.y S-V^V^c Material Tested xC\- Z & 6 A
bdt____ ____
Test OrganiseTe.Wftft>A Vw^>* ^ * U .
. Pete Rec*d. .7-79 Batch No. %
Avg. Wt. ,G4 -t .2. grew Avg. Site 4 . M 3N No. Organites per dose
\A______
Test Solution Volueetfi Liters/All-Glass Twk Tank D1nansions: 36x21 ca sad 2A cm deep
Tiae Started Z- 0 O P w Date 8tarted 7.1 P-74 Date Coenleted
7 - I4 .7
Dilution Water Carbon-filtered well eater (Tew. 2 D C.. D.O. 7./ ppa, pH S,g '
InvestigatorlV V
(Full Signature1}
Ca YTPlO S
(ps% Confidence Llnitsl/12 S
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Control 1-6 A z o 2 Cbb
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D.O. Total
D.O. Total
1.0. [Total
Added No. Dead pH PMS No.Dead pH PP*. No.Deed & P M No.Dead
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Test Teap. C. Signature 6 Date Coaaents:
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Effects other than Mortality
Init. Cone.
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Syaptoas or Reactions
Control
Signature Date
Comments :
^SO-
24-Hr.
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. -r 4 6 ^ ^7 7 --5--
Total No. Affected
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3M ENVIRONMENTAL LABORATORY
.PROBIT ANALYSIS FOP BI-DASSAY DATA**
ND OF -CURVES 4 NO. OF OOHC. FOP CURVE 1 ? 4 INPUT- PATA-GONO.rNO. EXPOSED NO. DEAD
' 560rl6-0
?? ?lo50orol6.irblr-a
7 135016rll
I'DNNENTS FOP CUPPS 1
* F0/306A. F IS H
CONG.-
--L06
-GONG.-
FRACTION .... RESPONDING
-FITTED FRACTION
560 ........... a. 748198
1705000........................33..8.7..5.0.6..1
1350 .......... 3.130334
CHI-- SO PROBABILITY is >
0. 05
0 ............ 0.001130384 0. 0 6 3 5 .......0. 03755678 0. 1 6 5 ........0.3119693 0.6875 ....... 0.6433131
^ N O ' B F ........ INTERCEPT.... SL O P E ........ CHI SQ FOR -CHI- -SQ FOP ITERATIONS.... < A > ............<B>..........LIHEARITY.... PROBABILITY
. -6 ......... -87.65447 ..... 8.951618 ; -1.608148 ..... 0.447503
HETEROSENIETY -FACTOR <H> -1.............. 6 - 0.3756303
.JtC-50 - ...... 1338. 363
95 -POT.- -LDWER GDNF. -L-INIT- - ............... 1098.336 95 PCT. -UPPER CONF. -L-INIT - ............... 1485. 013
HO.- -OF -CONG.- -FDR -CURVE -3- ? -5
L INPUT DATA-CONC-.-rNO-EXPOSEDrNO. DEAD
>- -? -560rl6r1
`' ' -
f " -7 -?50rt6r3
f -7-1000rl6r3
?I350rl6rl0
C0HH6NTS -FDR CURVE 3 .-? FC -306A -FISH
........ LOG.... . -FRACTION.... FITTED CONG........ CONG.-.... -RESPONDING -FRACTION
430 ......... 3.633349--- -0 .... .... 0.0053318648 560....... -3.748188 --- - 0.0635 ....... 0.0381354 750......... 3.-875061---- -0.135 ... ... 0.1059364 1000..... - 3 ........... 0.135 ..... --0.3749694 1350 ........ 3.130334 ... 0.635 ........ 0.5333085 -
~ OHI-SO PROBABILITY -IS > 0.05
NO -OF........ INTERCEPT.... S L O P E ........ CHI SO -FOR --- CHI SQ FOR ITERATIONS -<B>...... - >........ LINEARITY--- -PROBABILITY
5 .......... -16.31943 -- -5.307193
-3.195753---- - 0.3634176
HETER06ENIETY -FACTOR H> - -1....... .... 6 - -0.3767633
LC-50 --
--- 1303.605 -
_
J
95 PCT.- LOWER -CONF. 1-IH1T -- ........ ;* 1.094.373 95 PCT.- UPPER CONF.- L-INIT -- ------ ------- 1939.-568
\A
NO.- OF -CONO. -FOR -CURVE -3 ? 3 INPUT -DATA--CONG.-rNO.--EXPOSEDrNO.-DEAD
? l-000r20.-0 ? 1350r20.1 ? -1800r20r6
"trs . COMMENTS -FOP -CURVE 3
? DAPHNIA FC -206A
2^
............. L O S ........ FRAOTION FITTED CONO......... CONG......... RESPONDING FRACTION
1-000............3 ........ ..........0............. 0.001701208
1350 .........3.1-30334 ..... 0.03 ...... 0.04492807 1800 ........ 3.-23S273..... 0.3....... -0.-3034318
CHI'SCI PROBABILITY -IS > 0. 03
I
NG O F ....................INTERCEPT...........SLOPE.................... C H I -SO -FOR------ C H I -SO FOR
I-PERAT IONS
< A > .................... <B>...................... L IN E A R IT Y ------ -PROBABILITY
6 .......... -31. 30478--- -9.-458595 .....0.04704249 --- 0.8282919
hETEROGENIETV FACTOR CH>- 1 .............6 -- 0.6613563
L-C-50 - ....... 2040.1-61
9925
-POT.-POT.-
C' G. OF
LOWER -UPPER -CONO.
-CONF.- LIM IT
-CONF.- -L IM FOR -CURVE
I-T4
- .....................................
-9...-.3................................
1573
86
24..--883861
INPUT DATA-CONC.rND. -EXPOSED rNO. DEAD
? 1000r20.0
? 1350r20. 1
? -1800r20.5
COMMENTS FOR -CURVE -4
7 -DAPHNIA -FC 206A
CD m
3
oo
............................. LOS <.............. FR A C T IO N .............. FITTE D OONC....................... CONO.- .......... RESPONDING FRACTION
C11R1083I00-3S0000............P.......R........O.........B.........A.........B.........I...L333IT..2.Y1..5.3.5-.0.I.2S3..7.3..34.>.
.-.0....-..0.-.5.-....000
.
.
0.2..55.......................................................................000...02045034206464128577615
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-*HO-OF--'-'"iiv
ITERATIONS - :' TA>
-CHI*> CHI SO FOR I " * LINEARITY -PROBABILITY | T l
V ......... -28.18185 - --- 8.-4345?
0.07407743 0.78349
HETEROGENIETV -FACTOR -<H> 1 .................................. G 0 .7 8 3 4 5 1 2
LC- 5 0 -- .................2154.-405
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POT.PCT.
-L-DWER -CONF.- -L IM IT UPPER -CONF. -L IM IT
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