Document KRpnaoGN2qaxqNNdn7bRNGqBr
AR 226.0704
ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA)
IEsTsuBSTANCE
Identity: Mixture containing perfluorooctanesulfonate which may also be r(e1f-eOrcrteadnteosualsfoPnFiOc Sac,idF,C-1,915,,2.o2r.3a,s3,a4,c4o.5m,p5o,n6,e6n,t7,o7f.8F,C8,-82-01F. heptadecafiuoro-, potassium salt, CAS # 2795-39-3)
Remarks: The 3M production lot number was 1. The test sample is FmiCx-t2u0r1eFof(F3-.101%12P9F).OSC,ur3r6e.n0t%indfioertmhaytlieonne ignldyiccoaltbeusttyhlaettFheCr-,23061.F6i%s waater, 8.25% acrylic foamer, 7.0% sodium octyl sulfate, 6.0% sulfonated alkyl ether sodium salts, 3.0% triethanolamine, and 0.15% tolyl triazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect toxicityofthe fluorochemical component of the test sample.
METHOD:
Method: OECD Guideline 202
Type: Acute static
GLP: No
Year completed: 1992
SSuppepcliieesr::
Daphnia magna In-house cultures,
maintained
at
AScl-DETD,
Duluth,
MN.
Analytical monitoring: Temperature, pH, conductivity and DO
Exposure period: 48-hours
Test organism age: Statistical method:
<24 EC:
hours old neonates calculated by Trimmed
Spearman-Karber
method. NOEC calculated using Dunnett's procedure.
Test conditions:
Dilution Dilution
wwaatteerr:cShheamlilsotwryw:ell
water
from
Two
Harbors,
MN.
HAlakradlinneistsy:: 117407 mmgg//LL ((aass CCaaCCOOss))
pH: 8.61
Lighting: Cool-white fluorescent lights providing a daily photoperiod of 16
hours light and 8 hours dark
Stock and test solution preparation: A 1000 mglL primary stock
solution was prepared by dissolving 1000 mg FC-201F in one Lofdilution ~~~
water. Remaining concentrations prepared by serial dilutionsof the stock
solution.
000348
tEexsptossoluurtieonv.essels: 250-mL borosilicate glass beakers containing 200 mL
Number of replicates: four
NNuummbbeerrooff dcoanpchennitdrsatpieornsr:epfliivceatpel:us5a blank control Water Ccohnedmuicsttirvyitdyurrianngget:he(0st4u8dyh:ours)
330 349 mhos/om (controls)
371 -- 376 pmhos/cm (1,000 mg/L exposure) pH range (0-48 hours):
88..5417 -88..4618 ((c1o,n0t0r0olmsg)/L exposure) `Temperature range (048 hours):
20.4 -20.6 C (all exposures) Dissolved oxygen range (0-48 hours): _
7.5-8.0 mg/L (controls) 7.18.0 mg/L (1,000 mg/L exposure)
R0 esu0 lts 00000000000 Nominal concentrations: Blank control, 62.5, 125, 250, 500, and 1000
moll.
Element values: 2484--hhoouurr EECCss == >310,80(0207m2g-/3L48) mg/L 48-hour NOEC = 125 mg/L.
Element values based on nominal concentrations
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and notthe fluorochemical proportion alone.
conclusions
000000000
TcohnefiFdCe-n2c0e1IFnt4e8rv-ahlooufr 2E7C2s;tow3a4s8dmegt/eLr.miTnehde t4o8-bheo3u0r8NmogO/bLsweirtvhead 9Ef5f%ect Concentration (NOEC) was 125 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331,
St. Paul, Minnesota, 55133
DATA QUALITY
--
tReesltiianbgi.litHyo:weKvleirmi,stchhersanakmipnlge=pu2.riTtyhiwsasstundoyt pmreoeptesrltyhcehcarriatecrtiearfiozredquaalnidty
000349
the study lacks analytical confirmation of the amount of fluorochemical proportion in solution.
REFERENCES
00000000
TTheisstisntguDdiyviwsaison,coDnudluutcht,edMbNyaAtStchle Croerqpuoersattoifont,heDu3lMuthCoEmnpviarnoyn,meLnatbal Request number K1792, 1992. o0 mer 00000 Last changed: 6/26/00
000350
ASABsRetopcoosvp0orTOis1o5iOinnV/DAADiBSuevkekaL. ASTSty 050001501
STUDY TITLE
:
ACUTE TOXICITY OF FC-201F TO DAPHNIA MAGNA
DATA STANDARD OECD GUIDELINE 202
STUDY COMPLETED August 21, 1992
TESTING FACILITY AScI-Duluth EAnSvciIronCmoernptoarlatiToensting Division
112 DuElaustth,SeMcNond558S0t5reet
Tel. No. (218) 722-4040
STUDYIDENTIFICATION NUMBERS AScI Study IDF 5030-015-01
3M Company Lab Request? K1792
SSpo pS3toyCDaOa:KsyI
1
000351
S a sT stsmms ia rseean
1.0 GENERAL INFORMATION
Study Title
I
Acute Toxicity of FC-201F to
Daphnia magna
I Data Standard
Sponsor
OECD Guideline 202.
|
o23nM435EE0sn0tv0oi,sr.on3m55ent`BaulshLaAbvoernaet,oryB,t. BPuialud,ing
| sponsors | Representative
LSaubsoarnatA.oryB,eacBhu,ild3iKngEnv2i-r3Eo-n0m9e,ntal
{
935 Bush Avenue, St. Paul, MN 55106;
1 Sponsor's Tab Remuest | TKeil732N.o. (612) 778-7452.
Testing Facility
Principal Investigator
{ Project pirector
Hi '5508; Tete Nev (318) 733-4040. AScI Corporation/AScI-Duluth
Environmental Testing Division, 112 East Second Street, Duluth,
Dinesh Vaishnav
Testing Facility
Donald Mount
Di irector
i
Definitive Test Dates
August 17-19, 1992.
{
| RDeaptoartValRiedvaiteiwon and
200g) Dinesh Vaishnav .
MQ Loo Report Signature:
ON;
yA
f RaentdenFtiniaoln RofepoRratw Data cTwoomplyeetaerds. from the date study is
aLnodcatFiionnal oRfeRpaowrtData
AESncvIiroCnomrepnotraaltioTne/sAtSicnIg-DDuivliustihon,
112 East Second Street, Duluth,
{
|wN 55805; Tel. No. (218) 722-4040. |
p-- tt
:
000352
ASETARctocvrpe0inT0io5a oDguDnvn nRsbOnae "AST uty DO 051501
2.0 OBJECTIVE(S)
Tcooncdeentterramtiinoen)theof48t-hhe EtCe5s0t asunbdst4a8n-che,NOEFCC-2(0n1oF,obsfeorrved effect Daphnia magna. rTheitsainsetdudybywatshecoSnpdouncstoerdanadccOoErCdDinGguitdoeltihneeAS2c02I s(tOuEdCDy p1l98a4n).
TSeusbtstance oTregsatnisms DWialtuetrion
3.0 TEST METHODS
rPreolpaetritvieesd:ensFiCt-2y011F.,1 o(rwaantgeer/r=ed1),liqsutiadb,le, and miscible with water. cToensttroclo)n,cen6t2.r5a,tio1n2s5:, 205.00, (5d0i0l,utainodn w1a,t0e0r0 m1g,/0L0,0 mpgr/eLparsetdockbysoalusteiroina.l Tdhirlouutgihoonutofthe atespte,rsiaslltenttestfoasomluattiotnhse aspuprfeaacree.d clear with DSoauprhcnei:a mHeaaglntahyn,eonlaetsessthoabntai2n4e-dh oflrdom ASI stock culture. dUsielutiinonteswta:terGr2a4vidh.abdeuflotrse tihseoltaetsetd in 4inriteipaltiicoant.es/Utseesdt 5 coonrcgeanntirsamtsi/orne.plHiacnadtleedanwdith oafsm6oommt.h Dboirdenoptipefteedwitduhrianng itnhseidetesdti.ameter TSowuorcHea:rboSrhsa,llMowinnweesloltawataerera.frTohmetmheost recent chemical analysis is in Raw Data Package. C1h7a0ramcgt/eLri(satsicCsa:CO;A)t atnedsta tpiHmeo,f a8.h6a1r.dnAeesrsatoefd for 24 h prior to using in the test.
SSpep S3tMyCDoo mAuRnyI?
3
000353
5copniouDAukS AArDty r0 B0s0a1500
CEhxapmobseurrse [ zncubation
Type: 250-ml borosilicate glass beakers.
csUosoveleurtieindo,nt/ecsehtxa:cmebp2et0r0.wmhleDnuroefixnpgdeirtlihumeteinottenastlw,atoekbrespetrovra-test |
tions were made.
I
Duration: 48 h.
mDaaiinltyaipnhoetdopuesriinogd:coo1l6 whhiltieghtfluanodres8cehntdark, lamps.
l
Temperature: 20.4-20.6C.
Observations |eBxipoolsougriecalc:hamDbaeirlsy-andgetnhtelny caoguinttaetded the
|
i(mimmomboibliilziezeddanodrgdaenaidsmstewsetreortghaonsiesmsthat did
cnohtambsewrism wweirtehingen1t5lyseacgointdasteadf)t.erDiedxponosture l
|
crheammobveersanyatda24phhn.ids from the exposure
|
Wtaottaelr hcahredminsetsrsy:and(1)alkAatlitneistty,ini(2t)iadtaiiolny--
tteermmpiernaattuiroen,= DaOn,d p(H3,) aatndtesspteciifniictiation and
1
acocncdourcdtiinvgittyo. APAHlAl mdeettheordmsina(t1i9o89n)s. were made {
|[| cEnadlpcouilnattions | E(CH5a0m:ilTtronimmete.d Salp.ear19m7a7n)-.Karber method
sNOtEiCc:alDsuonfntewtatr'es, pVreorcseidounre3.(1T,OXUSnTiATversstiattyi-of |
L
Wyoming 1989).
SSppooorrtyCaDEmRpIy
4
000354
AAeSeercrvoIipTOa1on5 gnDSioDLirneOon0aAsk A ty 0 001501
3.0 RESULTS
[ T(ePsetrtsiunbesnttandcaetaToaxreiciitnyTable 1) 4284--hh EECC5500:: 3>018000mg/nLg./L.
48-h NOEC: 125 mg/L.
{
PRaarnagmeesteorfsWater Chemistry
I | I_
mTgo/tLa)l:ha1r7d0n-1e8s6s. (as Caco, A1l47k-a1l5i2n.ity (as Caco, mg/L): Temperature (C): 20.4-20.6C Do (mg/L): 7.1-8.2. pH: 8.47-8.64. |(Supmehcoisf/iccm)co:3nd3u0c-t3i7v2i.ty
4.0 TEST QA/QC
ang criteria
[oan
oTresttraoprpgeadnisimn dceoandt,rolimemxopboisluizreed | t|0r%apdpeeadd., immobilized or
must not be more than 10%.
| tTheestltoewersmtinDaOtiocnonmcuesnttrabteioant at || vlaelauset a6t0%toefstttheempaeirratsuarteu.ration
m|TehaesulroewdestwasDO80c%oncofenttrheataiiorn
[saturation value at 20.4C.
[ Test auration must ve 48 h.
The test duration was 48 h.
tReesfteren(ctehissubtessttancies otpotxiiocniatly). | rFeicnealntretpesotrtwiftohr sthoediummost
_
_
___ |pcahclkoargied.e is in Raw Data_
SSpposo3aMmCoBomaprr
5
000355
Ast omnisASTAs AA he DS0y 53S001A501
5.0 REFERENCES
AMemtehroidcsanfPorubtlhiec
Health Association (APHA). 1989. Standard Examination of Water and Wastewater. APHA,
Washington, D.C.
.
HSapmeialrtmoann-MK.aAr.b,erR.mCe.thoRdussfooranedstiR.mVa.tinThgumrsetdoina.n 1l9e7t7h.alTrimmed c1o1n:7c1e4n-t7r1a9t,ion in toxicity bioassays. Environ. Sci. Technol.
O1r9g81a/n1i9z8a4t.ioOnECfDorGuEicdoenloimniecsCfooorpeTreasttiionngaonfd DCehveemliocpalmse.ntOE(CODECD). Publication Information Center, Washington, D.C.
UPnhiyvseirosliotgyy aofndWyToomxiincgo.log1y989L.aboTroaxtsotrayt, VeDrespiaorntme3n.1t. ofFisZhoology
and Physiology. University of Wyoming, Laramie, WY.
|
Sspoprt3oyCsDomIepaE
6
000356
AAEstrceeorS epuses TSarmas
Table 1. Acute toxicity of FC-201F to 0. magna | nTioemnsait/naslubcsotnacnnce Ccrugmaunltaetmisv'e number and % of dead and immobilized
"Percentages ase in parenthesis.
Bfcfoarcrtesapnodndnion-get9f5a%ctcocnofnicdeenntcreatiinotnesrvwailtsh
lEC2S0 eandnNsoECcso[b( [ait00am 0 mo gr /t'[nr[oosteoconr fidei nce w intei rvale|
40h zes0
308 mq/1
27109-34842
"ECS values were calculated based on the tast substance nominal concentration.
Soa r Copeytm
7
000357
