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. AR 226 -- 0773 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) JEST~S ~ UBSTANCE Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-85, or as a component of FC-203FC. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203FC. Current information indicates it is a mixture of 0.85% PFOS, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite,0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test. sample. METHOD: Method: OECD Guideline 203 Type: Acute static range-finder GLP: No Year completed: 1997 Species: Pimephales promelas Supplier: Aquatic Biosystems, Inc., Fort Collins, CO. Analytical monitoring: Temperature, pH, and DO. Exposure period: 96-hours Statistical methods: Not given. Test fish age: Juveniles (128-132 days) . Length and weight: Average length = 23 mm Average weight = 0.09786 g (wet) Loading: 0.2936 g/L Pretreatment: None Test conditions: Dilution water: Carbon-filtered well water (3M Well #2, St. Paul, MN). Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Once. I Stability of the test chemical solutions: Not noted. Exposure vessels: 1.2L vessels containing 1.0Ltest solution. Number of replicates: One. Blank control duplicated. Number of fish per replicate: 3. Number of concentrations: three plus 2 blank controls `Water chemistry during the study: 001379 pH range (0-96 hours): 77..99-88..12 ((c1o0n0t0rolmge/xLpoesxuproes)ure) TDeismspoelrvaetduroexyrgaenngera(0n-g9e6 hours): 18.5 (0-96 hours): C 7.88.5 mg (control exposure) 4.78.7 mg/L (1000 mg/L exposure) R0 ESWts 00. Nominal concentrations: Blank control, 10, 100, and 1000 mg/L. Element values: 96-hour LCs =>1000 mg/L. Element values based on nominal concentrations. SRuebmsatraknsc:eTReestmianrgkwsafsielcdo. nTduhcetveadlounesthreepmoirxtteudraepapslydteosctrhiabtemdixitnutrhee aTnedstnot the fluorochemical proportion alone. conclusions The FC-203FC 96-hour LCs for fathead minnow was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 pataQuaury eRexlpioasbiulrietyc:onKcieinmtirsacthiornasnkainndg t=es3t. oTrhgaensitsumdsy. isThaerraengies-afilnadcikngo,f with limited dsotcuudmyelnatcaktsiaonna.lytTihcealscaomnpfilremapturiiotnyowftahsenoatmopruonpteorflyflcuhoarroacctheermiizceadl apnrodpotrhteion in the solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number R1335, 197. OTHER EE ---- ren Last changed: 6/26/00 001330 TYPE TEST: ENVIRONMENTAL LABORATORY 58.40 ACUTE TOXICITY T0 FATHEADMINNOW,PIMEPHALESPRONELAS STATIC \Y__ STATICRENEWAL w RI33poSfo AN Fu c GE Fine testsupsTance: FC 203 FC AFFE DILUTION WATER: CARGON-ILTEREDWELLWATER(34WELL #2, ST. PAUL, WN) Temp. *CpH __,DO. mg/L TEST FISHSOURCE: Ba of BiSyslems oaterecener: 2-18-93 msHAGE: 12%:13%Qaus AVGWROOTUgio.oMge AVG.SZE 23:03 em NO.OF FISHTEST CHAMBER: 3 LOADING RATE: 0.2936 : pe]LoLg on]"" Loe `TESTSOLUTION VOLUME: loc DATE INTIATED: 4-c4o9n7m Ts RCo ADDED sZEOFTESTVESSELS: 1.2L DATETERMINATED:> _4-1-47_1180 Tes : mem 2 z ? A --1 LL ro 1A Joop] tocong LEH ES Ee 1 LL Cr rrrrrrrr ed 1 [ERETuas r Sr m es wr l [En LC50 (95% C1) swocomgle COMMENTS ead AiaD A aOdh Es roofnShAAu-gS1hes8i1edcnhienaug.TCooonlc onwe7deD4nOted 1.3 Erno SECONDARYEFFECTS 5. cn: E =LotsofEAA: = eset.Ens SWng.H =Hemera avs: Tes BEST COPY AVAILABLE D(Tiocomsgles) olu 7 003381 Environmental Laboratory { we _Ri33S InitiDaaltse:: Ts _"4-1-97 Pa-ge Z of 3 96-Hour Acute Toxicity To Fathead Minnow, Pimephales Promelas i Loading Rate Data Sheet : Testsubstance:FC-303Fc AFF 1+ [2 J To oo ||o atd ygs w| 1- [ [5 eTlooiii2a3a3[aass || : [ [se s TTooooswiz[| aaso | w5= --7 e T Joo o6sos||ea2e s2 l|| 9 [oo%ed| 22 | '; 40 _[oOaoascnl| X77 LoadingRate Calculation: - Avg. wt. (g): 0.091% Std. Dev: co4eS 4 No. fishivessel;__3 8 Volvessel: _l& 0.29358. fen_L__L . Loading rate: 0-293 : 001382