Document KJk7Xp5GoKR8y6oM1n4y58wdw

TRADE SECRET AR226-3191 DuPont-8838 Study Title H-24944: Static, Acute, 96-Hour Screening Test to Pimephales promelas Laboratory Project ID: DuPont-8838 Author: Laurie D. Bouchelle Study Completed on : November 21,2001 Performing Laboratory: E.I. du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 Work Request Number: Study Code Number: - 1Company Sanitized. Does not contain TSCA CBI H-24944: Static, Acute, 96-Hour Screening Test to Pimephales promelas DuPont-8838 CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluation of data obtained from this study. Work Completed by: Laurie D. Bouchelle Associate Scientist Date LIssued by Study Director: X ./ Alan Samel, M.S. Research Ecotoxicologist Z / /0, \ / i. Date 2- -- -------- Iy w a i m i 4 C u i w e d kvi ouiu A ARS I r vtV M v D | H-24944: Static, Acute, 96-Hour Screening Test to Pimephales prometas STUDY INFORMATION Substance Tested: DuPont-8838 Haskell Number: 24944 Composition: Known Impurities: Physical Characteristics: Light brown liquid Sponsor: E. I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. In-Life Tnitiated/Completed: October 29, 2001 / November 2, 2001 -3 Company Sanitized. Does not contain TSC CBI H-24944: Static, Acute, 96-Hour Screening Test to Pimephales promelas DuPont-8838 SUMMARY The acute toxicity o f H-24944 to the fathead minnow, Pimephales promelas was determined in an unaerated, 96-hour, static test. The study was conducted with 4 concentrations o f H-24944 and a dilution water control at a mean temperature of 21.7C (range of 21.6-21.7C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and the 1,10 and 100 mg/L test concentrations were clear and colorless at test start. The 1000 mg/L test concentration was clear and pale tan in color at test start. All water quality parameters were within acceptable limits during the exposure. . Exposure of H-24944 to the water control and nominal H-24944 concentrations of 1,10,100, and 1000 mg/L resulted in 0,0, 0,0, and 0% mortality, respectively, at the end of 96 hours. No mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 1000 mg/L. The results are summarized as follows: Nominal concentrations of H-24944, mg/L dilution water control, 1, 10,100, and 1000 96-hour LCso for H-24944, based on > 1000 nominal concentrations, mg/L H-24944 exhibited low concern for aquatic hazard3in an unaerated, 96-hour, static, acute test using the fathead minnow, Pimephales promelas. Smrchek, J,, Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation o f data. Environmental Toxicology and Risk Assessment, A S T M ST P 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia. -4 Company sanitized. Does not contain TSCA CBJ H-24944: Static, Acute, 96-Hour Screening Test to Pimephales promelas DuPont-8838 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-24944 Concentration ____ (^mg/L)' ____ Water Control 1.0 10 100 1000 'Test Initiation (0 hours) Dissolved Oxygen (mg/L) 8.8 8.8 8.7 8.7 8.7 pH 7.7 7.6 7.6 7.5 7.4 Test Comoletion (96 hours) Dissolved Oxygen (mg/L)____ 7.9 7.9 7.6 7.9 7.5 pH 7.7 7.7 7.6 7.7 7.6 TABLE 2 MORTALITY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24944 Nominal H-24944 Concentration (mg/L) Water Control 1.0 10 100 1000 Number of Dead Test Organisms / Number of Test Organisms at Study Start 24 Hours 48 Hours 72 Hours 96 Hours 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 5 finmnanv Sanitized. Does not contain TSCA CBt