Document KJdZx49zD9eDjzKgeYRk9ndRX

110574 Ch-/rucal Abstracts ol. 71, 1-69 P*e lii with CHitCIIOAc or CIlj.ClIOlI and ro*,itified as 3-hydroxy- propylmcrcapturic acid (.Y-acetyl-.S-(.l-hydroxypropyl )-L-cys- teinc, 1), There was no evidence for tlic presence of allyhner- capturic acid (.V-acctyl-A'-allyl-L-cystcinc, 11) in the urine of these animals. II. however, was detected in the urine of rats injected s.c. with CHiiClICI. .V-allylelutathionc. and .5-allyl-L- cysteine. Rats treated with CHiiCHCl also excreted I. Mer- capturie acids apparently arise hy the reaction of allyl eompds. or their active dcrivs. with glutathione. BQJ.N' 110574p Metabolites of octoclothepine eliminated in human and rat urine. Queisnerova, Milena; Svatck, ii.; Metysova. Jirina (Res, Inst. I'harm. Uiochcin,, Prague. Czech.). Biochcm. J. 1969, 114(3), 339-42 (linfr). Four metaLioiitcs and unchanged octoclothepine were c.` td. with dichlorocthanc from the urine of humans given octoclothepine. These substances were isolated and purified by column and thin-layer cliromatog. By chro matographic, spcctropiiotonictric, and polarographic anal., unchanged octoclothepine and J of the metabolites were identified (noroctoelothcpine, noroetoelothepine .s-oxide, and octoclothe pine .S-oxide). The presence cf glacuronides in human urine was shown. The same metabolites and unchanged octoclothepine were also found in rat urine by cliromatog. RCFG 110575q Mechanism by which leucine and arginine stimulate insulin release in vitro. Milner, R. D. G. (Univ. West Indies, Kingston, Jamaica). Biochim. Biophys. Acta 1969, 193(1), 154-6 (Eng). Basal insulin release in young rabbit pancreas slices in vitro was higher in the presence of leueme (5mM) than of arginine (5m.'.r) (in the absence of glucose). Glucagon (5 pg./ml.), theophylline (lm.1/), dibutyryl cyclic AMP (lm.l/), or L-arginine (5m.!/) stimulated insulin release in the presence of leucine but not in the presence of arginine. Arginine-stimulated basal insulin release perhaps works by releasing glucagon from the a*cells. The mechanism of 'he leucine-induced stimulation is different, prob->Ny via the adcnyl cyclase system. BSJN 110576r Cytochemical study of the distribution and content of nudeoproteins and 't some functional groups of proteins in iso lated formations of the central nervous system following foreign protein injection. Tumanyan, E. L. (Erevan. Med. Inst., Erevan, USSR). Biol. Zh. Arm. I960, 33(5), 97-8 (Russ). Cytoplasm of various parts of rabbit central nervous system (including intervfteorai ganglia, nuclei of the XII cranial nerve, and skin motor ere i of brain cortex) displayed higher nbonuclco- profein content in mmals treated with repeated i.v. injection of normal horse serum. The peak effect was observed on the 4th day after the last injection. N changes were seen in content and distribution of protein SH groups. N\ Jasinczuk 110577s DNA synthesis in mouse embryonic fibroblast-like cells in vitro, stimulated by various factors. Vasii'ev, Yu. M.; Gel'fand, I. M.; Gel'shtem, V. I.; Fetisova, E. K. (Mosk. Gos. Uriiv,, Moscow, USSR). Dckl. Akad. ,\auk SSSR 1969. 187(4), 913-15[Cytol| (Russ). Autoradiography was used to follow the DNA synthesis in single-layer cultures of mouse embryo fibro blast-like cells in lactalbumin hydrolyzate and ox serum media in the presence of thymidine-1// added at various periods of culture age. The index of *H inclusion was increased by addn. of tes ticular or bacterial hyaluromdases, R\ase, and digitomn. The rise of *H intake was the result of stimulation of the entry of the cells into the S-phase of development. G. M. Kosolapoff U0578t Effect of pregnancy and oral contraceptives on glu cose and insulin metabolism. Taskinen, Marja R. Duodecim 1969, 85(11), 070-83 (Finnish). A review. The effect of both pregnancy and oral contraceptives is diabetogenous, and they may cause deterioration of glucose tolerance in healthy women. The organism tends to compensate this by enhanced insulin secretion, which leads to hyperinsulinism. 43 references. I. G. Zewi U0579u Effect of pyridozal 5'-phosphate on the strength of skin in vitro. Harkness. Robert D. (Univ. Coll. London, Lon don, Engl,), Eiperientia 1969, 35(10), 1048-9 (Eng). Treat ment with 10m,!/ pyridoxal 5'-phosphate (I) in vitro reduced the strength of rat tail skin progressively with time of incubation. The redn. was reversible, with strength returning when the skin rings were placed in buffered saline. Pyridoxamine or pyri- doxine at 10m.!/ had no comparable effect. The effect of 1 was reduced in the presence of lysine. Pretreatmcnt of the skin with NaBH4, followed hy washing in saline buffer, largely inhibited the effect of I. Pretreatmcnt with N'lpOII 1U0 inhibited the effect of I. Treatment with NTf;OII aficr I : resented the re covery of strength on subsequent transfer v- the saline buffer: HiNNHj had a similar effect. The effect ;>f I i skin strength is apparently brought about hy rupture of a ncllimc linkages formed between an aldehyde and an amitm gr ip. BSJN llOSSOn Diabetogenic effect of l.-aspc aginase. Khan, Amanull.ih; Adachi, Mitstio; lldt. Joseph M uGln-.lian (Wad- ley Inst, of Mol. Med., Dallas, Tex.). J. Lint. F.itihi, mini, ilelab. 1969, 39(10), 1373-0 fling). The effects of Esi hrrirhia toli l.-asparaginase (.'Vl.OtHl Iff1, kg., i.v.l, which lias been used to induce remission of liiiin.in mute ffoph.itic and myelog enous leukemias.via depletion of an ammo noil essential for Mime neoplastic cells, on rabbit blood and urinary gducusc levels were studied. A dose of 3fKM J. L-asparaginase/kg. evoked hyper glycemia and glycosuria in most of the animals; this is equal to a dose which induced hyperglycemia when given to patients. A If rabbits receiving . j.oou I.U. L-asparagmasc/kg. or more showed diabetogenic responses, but these effects were temporary (3-13 days) and were not axsoed. with any histological changes m the pancreatic islets, except in 1 rabbit which showed islet hyalmira tion. DDJ.N* 1105Slp Effect of Bidrin on the metabolism of dietary com ponents by the bovine. Lowrey, R. S.; Bowman, Malcolm C.; Knox, F. E. (Coastal Plain Exp. Sta., Agr. Res. Scrv., Tifton, Ca.). J. Dairy Sci. 1969, 53(9). 1400-3 (Eng). Six steers averaging 400 kg. in wt. were used in a switchback trial to study the effect of spiking corn silage with 15.7 ppm. (0.34 mg./kg. bi dy wt.) of the pesticide Bidrin (3-(dimethoxyphosphinyloxy)- iV,iV-dimethyl-fij-crotonamide] on blood cholinesterase, digest ibility of dietary components, utilization of N, and rumen fermen tation. Bidrin residues were not found either m the urine or m the feces of animals ingesting Bidrin. Azodrin. a metabolite of Bidrin, was not present m the feces of treated animals but aver aged 0.430 ppm. in the urine. Based on urine vol. this repre sented 1.5% of the ingested Bidrin. Blood cholinesterase was depressed to 30% of pretreatment values by the end of the 42-day exptl. period, whereas activity of steers fed untreated silage was unaffected. In the metabolism trial conducted during the last 14 days of the 43-day period Bidrin had no effect on the digest ibility of dietary components (dry matter, crude protein, ether ext., crude fiber, N-free ext., and gross energy), the utilization of N, or rumen fermentation. RCLD 110582q Effect of a second dose of cadmium salts on vascular permeability in the rat testis. Clegg, E. J.; Carr, Ian; Niemi, I.I. (Univ. Sheffield, Sheffield, Engl.). J. Eniocrincl. 1969, 45 (2), 265-8 (Eng). Two doses of CdCli were given to rats at intervals varying from 76 to 194 days. After the second dose the blood vessels in the testis and initial segment of the epididymis showed increased permeability, as demonstrated by i.v. markers. In the testis, leaking vessels were usually found in areas where interstitial cells were abundant. RCKV 110533r Acid-base and electrolyte changes induced by acute isotonic saline infusion in the nephrectomized dog. Rosenbaum. Barry J.; Makoff, Dwight L.; Maxwell, Morton H.; Hogh- oughi. Michael (Cedars-Sinai Med. Res. Inst., Los Angeles. Calif.). J. Lab. Clin. Afed. 1969, 74(3), 427-35 (Eng). The mechanism and extent of "dilution acidosis" by acute infusions of isotonic saline was studied in nephrectomized dogs. Infusions uf 50, 75, and 100 ml./kg. of body wt. (BW) (Groups l, 2. and 3) over a 30-min. period resulted in extracellular fluid expansions of 24.4 1.7%, 28.8 2.2%, and 33.9 5.5%, resp. pCOi was kept const. Body spaces were measured isotopically. and intra cellular H+ activity by 5.5-dimethyl-2,4-oxaxolidinedione-MC. The extracellular bicarbonate concn. decreased only --2.4 0.7 meq./l. and --2.5 0.4 meq./l. in Groups J and 2 and --3.0 1.0 in Group 3. The total amt. of extracellular bicarbonate in creased +0.6 0.2 meq./kg. of BW, +0.8 0.2 meq./kg. of BW, and +1.5 * 0.4 meq./kg. of BW in each group, resp. Con comitantly, the total amt. of extracellular fluid K increased +0-3 0.0 meq./kg. of BW, +0.5 0.1 meq./kg. of BW, and +0.4 0.1 meq./kg. of BW, resp.. while there was no decrease in extracellular K concn. It was suggested that the extracellular bi carbonate and K concns. were defended by shifts of H* into ceils (or bicarbonate out of cells), in conjunction with a shift of K out of cells. Despite the presumed H+ (or bicarbonate) flux, there were no net changes of H+ activity. The conceptual theory of extracellular "dilution acidosis" is valid but of limited magnitude and little clin. significance when expansion is relatively slow. Cellular buffering minimizes the extracellular acidosis, and the shift of K out of cells prevents hypokalemia. RCKY 110584s Effect of erythropoietin ou bone marrow a-amino levulinic acid synthetase and heme synthetase. Bottomley. Sylvia 5.; Smithcc, G. Ann (Veterans Admin. Hosp., Oklahoma City. Okla.). J. Lab. Clin. Med. 1969. 74(3), 445-52 (Eng) An in vitro culture method has been adapted for rabbit bone marrow and has permitted measurement of the effect of erythro poietin on A-aminolevulinic acid synthetase and heme synthetase activities in marrow cells. In this system, erythropoietin en hanced A-aminolcvulinic acid synthetase activity. This effect was detectable as early as 6 hrs. after contact with erythropoietin. The max. enzyme activity observed was 300% greater in stim ulated than in control cultures. Studies with inhibitors of pro- tein synthesis suggested that erythropoietin increased tne syn thesis of the enzyme. N'o stimulatory effect by erythropoietic on heme synthetase activity was observed. RCKY 110585t Action of adenosine triphosphate on the depre',3e : spontaneous electrical activity of the dog cerebral cortex. II; nr- Gian; Arriguiu, EU-na; Ferrara. A.: Mnselicipa, 1'. < I Pharmacol.. Univ. Pavia. Pavia, Italy). J. I'ha'm. ,S>>. 58(9), 1161 3 (Eng). The repeated ^oppression of ilu ventd. tioii and the c irculacion of the liraiit in the dog mdii ic u logit < ' pressnm or a silence of elcc. activity of the cortical ami cut cortical centers. The recovery of respiratory and circulator. BOR 009728