Document KJLkLeOpRQY7X3ZLEE3oxyBaw

ervkes" Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810b\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 02081 OSamplesLong.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 5.0ppb 2/8/2010 6:45:29 PM PFCkinetexLong.dam LCMS2-85D Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 5 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.39 (1.36) PFNA 1 2.30 (2.42) PFOS 1 2.36 (2.53) PFDA 1 3.34 (3.34) PFUnA 1 4.12(4.06) PFDS 1 4.13(4.10) PFDoA 1 4.76 (4.63) PFTrA 1 5.29 (5.14) PFTeA 1 5.76 (5.74) IS Name 13C-PFOA 13C-PFNA 13C-PFOS 13C-PFDA 13C-PFUnA 13C-PFOS 13C-PFDOA 13C-PFDoA 13C-PFDOA IS Area 2766100 2154600 6761100 2111500 172740 6761100 199680 199680 199680 Analyte Area 651680 1218400 3709300 1041800 848100 3957300 578950 408680 278340 Exp Cone (ng/mL) 5.000 5.000 5.000 5.000 5.000 5.000 5.000 5.000 5.000 Calc. Cone (ng/mL) 4.880 Accuracy (%) 98 4.960 99 5.160 103 4.700 94 5.190 104 5.060 101 5.300 106 5.780 116 5.190 104 Mod? No No No No No No No No No ^--i ( u d o Page 26 of 242 277 /% Columbia IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 p.2 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM RT (Expected) 1.38(1.36) 1.38(1.36) 2.34 (2.44) 2.30 (2.44) 2.35 (2.53) 2.36 (2.53) 3.32 (3.34) 3.31 (3.34) 4.12(4.06) 4.12(4.06) 4.13 (4.10) 4.76 (4.64) 4.76 (4.64) 5.29 (5.13) 5.74(5.56) Area 1629900 2766100 355520 2154600 1159300 6761100 156690 2111500 171520 172740 1260200 163950 199680 142130 108440 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.50 4.24 0.29 1.77 0.31 1.82 0.15 2.03 0.20 0.20 0.32 0.28 0.34 0.35 0.39 Page 27 of 242 278 / X C olum bia C ^ Analytical Quantitative Peak Review Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 5.0ppb 2.30 PFOA 1 413.0/169.0 Da q Baseline c Missed r' Wrnnn Peak ________ 13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak___ PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da d Baseline Missed n Wrnnn Peak _______ 3^1______ PFOS 1 499.0/80.0 Da Baseline Missed n W rnnn Peak 4,12 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak "'l PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Ppak B7**44-\| 4-,13 13C-PFDA 515.0/470.0 Da Baseline d Missed r Wrnnn Peak PFUnA 1 563.0/519.0 Da o Baseline d Missed ___n Wrnnn Peak___ 13C-PFUnA 565.0/270.0 Da Baseline c Missed ___i ' Wrnnn Peak___ PFDS 1 599.0/80.0 Da Baseline Missed ___n Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak PFDoA 1 613.0/569.0 Da c Baseline Missed r Wrnnn Peak .4 13C-PFD0A 615.0/169.0 Da Baseline Missed p Wrnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed ___ n W rnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTeA 1 713.0/669.0 Da Baseline Missed ___r Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da c Baseline Missed ___ r W rnnn Peak___ Before After 279 Page 28 of 242 ** Columbia Analytical Services" Ion Ratio Confirmation p. 4 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 5.0ppb PFOA2 413.0/369.0 Da g Baseline Missed g Wrong Peak PFUnA2 563.0/269.0 Da g Baseline Missed c Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFDS 2 599.0/99.0 Da Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da o Baseline o Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da a Baseline c Missed Wrong Peak SOU (M c 9 >> - ;....." PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 29 of 242 280 Columbia Analytical Created with Analyst ftefiorter Printed: 14/02/2010 6:41:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810b\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 02081 OSamplesLong.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 10ppb 2/8/2010 6:53:02 PM PFCkinetexLong.dam LCMS2-85E Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 6 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.34(1.36) IS Name 13C-PFOA IS Area 3016300 PFNA 1 2.29 (2.42) 13C-PFNA 2357300 PFOS 1 2.35 (2.53) 13C-PFOS 6895700 PFDA 1 3.34 (3.34) 13C-PFDA 2127100 PFUnA 1 4.12(4.06) 13C-PFUnA 172090 PFDS 1 4.12(4.10) 13C-PFOS 6895700 PFDoA 1 4.76 (4.63) 130-PFDoA 225140 PFTrA 1 5.29 (5.14) 130-PFDoA 225140 PFTeA 1 5.74 (5.74) 13C-PFDOA 225140 Analyte Area 1492100 2910500 8049100 2332600 1784200 8526300 1395800 925940 716720 Exp Cone (ng/mL) 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 Calc. Cone (ng/mL) 10.200 Accuracy (%) 102 10.800 108 11.000 110 10.400 104 11.000 110 10.900 109 11.400 114 11.700 117 11.900 119 / Mod? No No No No No No No No No Page 30 of 242 281 Columbia A n a ly tic a l IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 p. 6 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM RT (Expected) 1.35(1.36) 1.35(1.36) 2.30 (2.44) 2.28 (2.44) 2.34 (2.53) 2.33 (2.53) 3.34 (3.34) 3.35 (3.34) 4.12(4.06) 4.12(4.06) 4.12(4.10) 4.76 (4.64) 4.76 (4.64) 5.29 (5.13) 5.74 (5.56) Area 3733500 3016300 850370 2357300 2557600 6895700 349340 2127100 378600 172090 2766600 414280 225140 306170 228950 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.50 2.02 0.29 0.81 0.32 0.86 0.15 0.91 0.21 0.10 0.32 0.30 0.16 0.33 0.32 Page 31 of 242 282 Columbia Analytical Quantitative Peak Review 1.35 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 10ppb PFOA 1 413.0/169.0 Da c Baseline Missed h Wrnnn Peak PFOS 1 499.0/80.0 Da d Baseline Missed i Wronn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed -- nWronn Poal<___ :! T !1 13C-PFOS 503.0/80.0 Da g Baseline a Missed n Wrnnn Peak 4.12 PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak |1 5 IOtS S0J T L PFDA 1 513.0/469.0 Da Baseline o Missed n Wrnnn Peak 4.,12 13C-PFNA 468.0/423.0 Da Baseline Missed ___n Wronn Peak___ 13C-PFDA 515.0/470.0 Da d Baseline Missed n Wrnnn Peak 2.33 PFUnA 1 563.0/519.0 Da d Baseline Missed ___n Wrnnn Peak 13C-PFUnA 565.0/270.0 Da g Baseline c Missed ___ n W rnnn Peak___ PFDS 1 599.0/80.0 Da c Baseline n Missed ___n Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da g Baseline Missed ___p Wrnnn Peak___ 4.76 PFDoA 1 613.0/569.0 Da c Baseline Missed p Wrnnn Peak 5.74 13C-PFDOA 615.0/169.0 Da o Baseline Missed Wrnnn Peak PFTrAI 663.0/619.0 Da Baseline Missed n W rnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed ___p Wrnnn Peak___ i PFTeA 1 713.0/669.0 Da Baseline Missed ___ r W rnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed ___n Wrnnn Peak___ Before After Page 32 of 242 283 Ion Ratio Confirmation p. 8 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 10ppb PFOA2 413.0/369.0 Da c Baseline Missed g Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak PFNA 2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da o Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline a Missed Wrong Peak i .. k - T - r - . . : . Ii US ............. 4,p 6............ J -. ~ --J i : ...so \ PFDS2 599.0/99.0 Da Baseline Missed d Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak >5e`. 1 5. k ............ 1 S(1 S'. PFTrA 2 663.0/169.0 Da Baseline a Missed Wrong Peak .... 5,174... PFTeA2 713.0/169.0 Da g Baseline Missed Wrong Peak Before After Page 33 of 242 284 p. 9 Createci with Analyst Reporter Dia Printed: 14/02/2010 6:41:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 02081 OtACalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 02081 OSamplesLong.rdb InteiliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb 2/8/2010 7:00:35 PM PFCkinetexLong.dam LCMS2-85F Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 7 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.35 (1.36) PFNA 1 2.28 (2.42) PFOS 1 2.33 (2.53) PFDA 1 3.30 (3.34) PFUnA 1 4.12(4.06) PFDS 1 4.12(4.10) PFDoA 1 4.76 (4.63) PFTrA 1 5.28 (5.14) PFTeA 1 5.74 (5.74) IS Name 13C-PFOA 13C-PFNA 13C-PFOS 13C-PFDA 13C-PFUnA 13C-PFOS 13C-PFDoA 13C-PFDOA 13C-PFDOA IS Area 2888500 2279400 7033400 2039400 164330 7033400 225670 225670 225670 Analyte Area 2577000 4677900 14114000 4081100 2860500 14304000 2435400 1476200 1021200 Exp Cone (ng/mL) 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 Calc. Cone (ng/mL) 18.400 Accuracy (%) 92 17.900 90 18.800 94 19.000 95 18.400 92 18.500 93 19.800 99 18.600 93 17.100 85 / Mod? No No No No No No No No No 285 -Vf A \ i 2J t '- i r l i * Page 34 of 242 Z% Columbia *J Analytical Sarviea IRC Summary ; Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S13C-PFOS PFDA2 S13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Expected) 1.34(1.36) 1.35 (1.36) 2.29 (2.44) 2.29 (2.44) 2.32 (2.53) 2.32 (2.53) 3.29 (3.34) 3.33 (3.34) 4.11 (4.06) 4.12(4.06) 4.12(4.10) 4.76 (4.64) 4.76 (4.64) 5.28 (5.13) 5.74 (5.56) p. 10 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Area 6202500 2888500 1501800 2279400 4486000 7033400 632970 2039400 656460 164330 4656800 651490 225670 479330 356280 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.41 1.12 0.32 0.49 0.32 0.50 0.16 0.50 0.23 0.06 0.33 0.27 0.09 0.32 0.35 Page 35 of 242 286 Quantitative Peak Review p. 11 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 20ppb PFOA 1 413.0/169.0 Da Baseline Missed n W ronn Peak 13C-PFOA 417.0/372.0 Da c Baseline Missed ;Wrnnn Peak ^ ^ 2 ^ ^ ________ PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak i 13C-PFNA 468.0/423.0 Da c Baseline Missed n Wrnnn Peak PFOS 1 499.0/80.0 Da Baseline Missed n W rnnn Pnak 4.12 13C-PFOS 503.0/80.0 Da Baseline Missed W rnnn Ppak PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Pnak 4.12 i 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da c Baseline Missed ___n Wrnnn Peak___ 13C-PFUnA 565.0/270.0 Da d Baseline Missed n Wrnnn Peak aon, PFDS 1 599.0/80.0 Da o Baseline Missed __ r Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed _____ n Wrnnn Peak___ PFDnA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 5.74 13C-PFDnA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da Baseline c Missed ___ p W rnnn Peak___ 13C-PFDnA 615.0/169.0 Da Baseline o Missed n Wrnnn Pnak PFTeA 1 713.0/669.0 Da c Baseline Missed i- W rnnn Pnak 13C-PFD0A 615.0/169.0 Da Baseline Missed p Wrnnn Peak Before After 287 Page 36 of 242 Columbia A nalytical Ion Ratio Confirmation p. 12 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 20ppb PFOA2 413.0/369.0 Da Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed c Wrong Peak .3,2. Uk PFNA2 463.0/219.0 Da o Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da a Baseline Missed u Wrong Peak ..............4. -2............. ________ . L _ ___ PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak 8 W` M. _ 4. 6.. _ PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak | ! ( PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After 288 Page 37 of 242 Createci with A n a ly s fR ^ o rte r Printed: 14/02/2010 6:41:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 02081 Ob\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 02081 OSamplesLong.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 50ppb 2/8/2010 7:08:10 PM PFCkinetexLong.dam LCMS2-85G Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 8 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.38(1.36) IS Name 13C-PFOA PFNA 1 2.31 (2.42) 13C-PFNA PFOS 1 2.36 (2.53) 13C-PFOS PFDA 1 3.33 (3.34) 13C-PFDA PFUnA 1 4.12(4.06) 13C-PFUnA PFDS 1 4.12(4.10) 13C-PFOS PFDoA 1 4.76 (4.63) 13C-PFDoA PFTrA 1 5.30 (5.14) 13C-PFDOA PFTeA 1 5.73 (5.74) 13C-PFDOA IS Area 2896200 2153900 6959900 1935900 163690 6959900 227850 227850 227850 Analyte Area 7236800 12661000 37073000 10412000 7761500 33566000 6008800 3923300 2949500 Exp Cone (ng/mL) 50.000 50.000 50.000 50.000 50.000 50.000 50.000 50.000 50.000 Calc. Cone I Accuracy (ng/mL) (%) 51.500 103 51.300 | 103 50.000 100 50.900 102 50.300 . 101 50.500 101 48.500 97 48.900 98 50.800 102 Mod? No No No No No No No No No 289 -4' r ll Page 38 of 242 IRC Summary i Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 Created with AnalysPp^eporter Printed: 14/02/2010 6:41:00 PM RT (Expected) 1.38 (1.36) 1.38(1.36) 2.33 (2.44) 2.31 (2.44) 2.36 (2.53) 2.36 (2.53) 3.33 (3.34) 3.33 (3.34) 4.12(4.06) 4.13(4.06) 4.12(4.10) 4.76 (4.64) 4.75 (4.64) 5.29(5.13) 5.74(5.56) Area 17086000 2896200 3977000 2153900 12374000 6959900 1652600 1935900 1983000 163690 12510000 2079000 227850 1260000 1256900 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.36 0.40 0.31 0.17 0.33 0.19 0.16 0.19 0.26 0.02 0.37 0.35 0.04 0.32 0.43 Page 39 of 242 290 Quantitative Peak Review 1.38 1.38 p. 15 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 50ppb 2.31 PFOA 1 413.0/169.0 Da Baseline Missed r W ronn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed ___n Wronn Peak___ PFNA 1 463.0/419.0 Da o Baseline Missed n Wronn Peak 13C-PFNA 468.0/423.0 Da a Baseline Missed n Wronn Peak PFOS 1 499.0/80.0 Da Baseline q Missed n Wronn Peak PFUnA 1 563.0/519.0 Da Baseline Missed n W rnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n W rnnn Pnak 413 PFDA 1 513.0/469.0 Da Baseline Missed r, W ronn Paak 1 -** 4f 13C-PFUnA 565.0/270.0 Da Baseline Missed n W rnnn Peak I PFDS 1 599.0/80.0 Da Baseline d Missed Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak 4.7 PFDoA 1 613.0/569.0 Da Baseline a Missed n W rnnn Pnak 'M 13C-PFDOA 615.0/169.0 Da Baseline Missed n W rnnn Peak PFTrA 1 663.0/619.0 Da d Baseline Missed p Wrnnn Peak 13C-PFDOA 615.0/169.0 Da c Baseline o Missed ___ p W rnnn Peak___ PFTeA 1 713.0/669.0 Da c Baseline o Missed n W rnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed ___ p W rnnn Peak___ Before After Page 40 of 242 291 Ion Ratio Confirmation p. 16 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM PFC 50ppb PFOA2 413.0/369.0 Da u Baseline Missed c Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed o Wrong Peak U 40 4S ill PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da o Baseline Missed o Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed c Wrong Peak #*5j M| 1 .............. 5-, 9 - ; ............ PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da o Baseline o Missed o Wrong Peak PFTeA2 713.0/169.0 Da l Baseline u Missed Wrong Peak Before After Page 41 of 242 292 p. 17 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 02081 OfcACalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 02081 OSamplesLong.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb ICV 2/8/2010 7:30:54 PM PFCkinetexLong.dam LCMS2-77J Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 9 5 Quality Control 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.38 (1.36) IS Name 13C-PFOA PFNA1 2.33 (2.42) 13C-PFNA PFOS 1 2.36 (2.53) 13C-PFOS PFDA 1 3.33(3.34) 13C-PFDA PFUnA 1 4.11 (4.06) 13C-PFUnA PFDS 1 4.12(4.10) 13C-PFOS PFDoA 1 4.76 (4.63) 13C-PFDOA PFTrA 1 5.29(5.14) 13C-PFDOA PFTeA 1 5.74 (5.74) 13C-PFDOA IS Area 2771800 2335500 6969700 1968300 154400 6969700 217390 217390 217390 Analyte Area 2843400 5206100 15447000 4333800 3245900 15777000 2671400 1712600 1043200 Exp Cone (ng/mL) 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 Calc. Cone (ng/mL) 21.200 Accuracy (%) 106 19.500 97 20.800 104 20.900 104 22.300 111 20.800 104 22.600 113 22.400 112 18.100 91 / Mod? No No No No No No No No No 293 =& u l io /1 Page 46 of 242 IRC Summary i Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 p. 18 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM RT (Exp R T ) 1.39(1.36) 1.40(1.36) 2.33 (2.44) 2.31 (2.44) 2.37 (2.53) 2.36(2.53) 3.33 (3.34) 3.32(3.34) 4.13(4.06) 4.12(4.06) 4.12 (4.10) 4.77 (4.64) 4.75 (4.64) 5.29(5.13) 5.74(5.56) Area 6554000 2771800 1566300 2335500 4995900 6969700 656620 1968300 755100 154400 5118100 808950 217390 600370 411830 Modified? No No No No No No No No No No No No No No No Ratio Confirms ID YES No YES No YES No YES No YES No YES YES No YES YES Page 47 of 242 294 Quantitative Peak Review Created with Analyst Fie^brter Printed: 14/02/2010 6:41:00 PM PFC 20ppb ICV Before After 295 Page 48 of 242 Columbia Analytical Ion Ratio Confirmation Created with Analyil'Reporter Printed: 14/02/2010 6:41:00 PM PFC 20ppb ICV PFOA2 413.0/369.0 Da c Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da q Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed o Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak V '\ 1 1'J *' t 4V PFUnA2 563.0/269.0 Da c Baseline Missed Wrong Peak , ! & ** .............. 4. 2............... 4, 7 - I ............. 5. S ............ r> tu t` r.i) PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak M 5 V- PFDoA2 613.0/169.0 Da Baseline o Missed Wrong Peak PFTrA2 663.0/169.0 Da d Baseline c Missed Wrong Peak ? - 5; 4........... ' r i ______ j 1 PFTe/\ 2 713.0/16}.0 Da c Baseline 3 Missed c Wrong Peak Before After Page 49 of 242 296 p. 21 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Analyte Name: Internal Standard: PFBA 1 13C-PFBA Data File Acquisition Date Acquisition Method Project 020810\CalShort.wiff 2/9/2010 3:06:42 AM PFCkinetexShort.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Regression Equation:________y = 0.106 x + 0.00604 (r = 0,9986' Exp Cone (ng/mL) # of Values Mean Calc Conc (ng/mL) 0.1 1 0.09 0.5 1 0.49 1 1 1.05 5 1 5.33 10 1 11.09 20 1 18.67 50 1 49.88 % Accuracy 87 98 105 107 111 93 100 r STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN Page 1 of 245 297 p. 22 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Analyte Name: Internal Standard: PFPeA 1 13C-PFHxA Data File Acquisition Date Acquisition Method Project Regression Equation: Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 020810\CalShort.wiff Result Table 02081 OSamplesShortedit.rdb 2/9/2010 3:06:42 AM Algorithm Used IntelliQuan PFCkinetexShort.dam Instrument Name API 5000 Ewan's Projects\PFCs 7y = 0.0955 x + 0.002 (r = 0.9976) # of Values Mean Calc Cone (ng/mL) % Accuracy STD %CV 1 0.07 73 NaN NaN 1 0.49 99 NaN NaN 1 1.10 110 NaN NaN 1 5.50 110 NaN NaN 1 11.59 116 NaN NaN 1 19.02 95 NaN NaN 1 48.83 98 NaN NaN 7 298 37 38 19 -{(0 Page 2 of 245 Created with AnalysP'Rejxirter Printed: 15/02/2010 2:34:00 PM Analyte Name: Internal Standard: PFBS 1 180-PFHS Data File Acquisition Date Acquisition Method Project 020810\CalShort.wiff 2/9/2010 3:06:42 AM PFCkinetexShort.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/ml_) 0.1 0.5 1 5 10 20 50 y = -0.00191 xA2 + 0.354 x + -0,00625 (r = 0.9989) # of Values Mean Calc Cone (ng/mL) % Accuracy 1 0.09 94 1 0.49 99 1 1.03 103 1 5.16 103 1 10.75 107 1 18.57 93 1 50.67 101 STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN / ' 3 i 5 7 6 '> 10 n 1? 13 H ' 5 16 U 1 19 21 2? 2 25 2? 28 ? W AooM* ' one/ 1CCo: 22 )) 3< W * 3) 11 <1 H ? -8 9 Page 3 of 245 299 p. 24 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Analyte Name: Internal Standard: PFHxA 1 13C-PFHxA Data File Acquisition Date Acquisition Method Project Regression Equation: Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 020810\CalShort.wiff 2/9/2010 3:06:42 AM PFCkinetexShort.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 y = -0.000377 xA2 + 0.108 x + 0.00572 (r = 0.9988) / # of Values Mean Calc Cone (ng/mL) % Accuracy 1 0.10 97 1 0.49 98 1 1.01 101 1 5.09 102 1 10.91 109 1 18.56 93 1 50.50 101 T STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN s n I? ie i 9 ;i n ?j ;< 2? ioa*yiConc /fSCont 59 3i x 3) v av a *ts u o 300 Page 4 o f 245 Created with AnalysPF&^orter Printed: 15/02/2010 2:34:00 PM Analyte Name: Internal Standard: PFHpA 1 13C-PFHxA Data File Acquisition Date Acquisition Method Project Regression Equation Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 020810\CalShort.wiff 2/9/2010 3:06:42 AM PFCkinetexShort.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 y = -1,93e-005 xA2 + 0.0388 x + 0.00229 (r = 0.9986) / # of Values Mean Calc Cone (ng/mL) % Accuracy 1 0.09 94 1 0.51 101 1 0.96 96 1 5.32 106 1 10.88 109 1 18.39 92 1 50.45 10} STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN kE________________________ ______ ______________________________________________ ______ __________ -- l ? 3* t s M io l i 2 U u '9 H 19 I ' D <1 2? 23 ? 2 29 2? J9 39 31 32 33 34 K 3? fi 40 <3 *2 43 44 47 46 AtiotvwUne /iSConi ^ U Page 5 of 245 301 Servio' p. 26 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Analyte Name: Internal Standard: PFHxS 1 180-PFHS Data File Acquisition Date Acquisition Method Project 020810\CalShort.wiff 2/9/2010 3:06:42 AM PFCkinetexShort.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 y = -0.00198 xA2 + 0.335 x + 0.00261 (r= 0.9986) # of Values Mean Calc Cone (ng/mL) % Accuracy 1 0.10 97 1 0.48 95 1 1.03 103 1 5.09 102 1 10.91 109 1 18.44 92 1 50.77 1027 STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN Anety Cone yIS Cone 302 Page 6 of 245 Created with AnalysPf^porter Printed: 15/02/2010 2:34:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 020810\CalShort.wiff 02081 OSamplesShortedit.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial IB 2/9/2010 3:06:42 AM PFCkinetexShort.dam 1 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFBA 1 13C-PFBA PFPeA 1 130-PFHxA PFBS 1 18 0 -P F H S PFHxA 1 130-PFHxA PFHpA 1 13C-PFHxA PFHxS 1 180-PFHS S_180-PFHS 13C-PFDA SJ3C-PFDA 13C-PFDA IS Area 0 0 0 0 0 0 0 0 Analyte RT (ExpRT) 0.00(1.27) 0.00 (3.60) 0.00 (4.26) 5.34 (5.31) 6.23 (6.23) 0.00 (6.32) 0.00 (6.31) 0.00 (7.65) Analyte Area 0 0 0 44083 13368 0 0 0 Calc. Cone (ng/mL) N/A N/A N/A N/A N/A N/A N/A N/A Modified? No No No No No No No No i 3/1* 303 p. 28 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA RT(ExpRT) 0.00(1.27) 0.00 (3.60) 0.00 (4.26) 0.00 (5.31) 6.23 (6.22) 0.00 (6.32) 0.00(1.26) 0.00 (5.30) Area 0 0 0 0 29758 0 0 0 Modified? Calc (Exp) MRM Ratio No 0.00(1.00) Ratio Confirms ID No NO 0.00(1.00) No No 0.00 (0.40) No No 0.00 (0.10) No No 2.23 (3.03) No No 0.00(0.40) No No 0.00 (13.55) No No 0.00(13.06) No 304 Page 10 of 245 Quantitative Peak Review p. 29 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM IB PFBA 1 213.0/169.0 Da Baseline Missed n W rnnn Peak 35 4 45 50 PFBS 1 299.0/80.0 Da Baseline Missed n W rnnn Peak 15 20 13C-PFBA 217.0/172.0 Da Baseline o Missed n Wrnnn Peak PFPeA 1 263.0/219.0 Da d Baseline o Missed _____ n Wronn Peak___ !, I 5 jjf fijl \ , W jl }jl Ji( | # H r f i i jj1 55 6a 65 70 18 0 -P F H S 403.0/103.0 Da Baseline Missed n Wrnnn Peak 45 50 65 60 PFHx a I 313.0/269.0 Da Baseline Missed Wrnnn Peak Vk l v A / ' - , 60 65 70 PFHpA 1 363.0/169.0 Da Baseline Missed n Wrnnn Pnak 45 60 55 60 13C-PFHxA 315.0/270.0 Da Baseline Missed n W rnnn P<=ak 55 6D 66 70 P F H x il 399.0/80.0 Da Baseline Missed n Wrnnn Peak 130-PFHxA 315.0/270.0 Da d Baseline Missed >eak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak 5 W r y v 18 0 -P F H S 403.0/103.0 Da c Baseline Missed n Wrnnn Peak 56 60 S_180-PFHS 403.0/103.0 Da o Baseline Missed n W rnnn Peak 70 75 80 5 13C-PFDA 515.0/470.0 Da Baseline Missed _____ n W rnnn Peak___ 60 65 S_13C-PFDA 515.0/470.0 Da Baseline a Missed n Wrnnn Peak Before After 5ft 70 75 80 Tune trail 13C-PFDA 85 515.0/470.0 Da Baseline Missed n W rnnn P nak_____ Page 11 o f 245 305 lumbia p. 30 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 115 ia 15 rr, PFBA2 213.0/169.0 Da Baseline Missed d Wrong Peak . siII 3e* * 184 30 15 a 45 PFPeA2 263.0/219.0 Da O Baseline Missed Wrong Peak 6.23 IB >0o# 18 *TM3140000 15 40Timemtn45 50 PFBS2 299.0/99.0 Da Baseline Missed o Wrong Peak 4000 1000 3tift ?ooo \m 45 50 55 50 PFHx a "2 313.0/119.0 Da a Baseline Missed o Wrong Peak PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline o Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 12 o f 245 306 p. 31 Createci with Analyst Reporter Printed: 15/02/2010 2:34:00 PM 'K - Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 0.10ppb 2/9/2010 3:35:22 AM PFCkinetexShort.dam LCMS2-85A Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 2 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA 1 1.26 (1.27) PFPeA 1 3.60 (3.60) PFBS 1 4.26 (4.26) PFHxA 1 5.31 (5.31) PFHpA 1 6.24 (6.23) PFHxS 1 S 180PFHS S 13CPFDA 6.33 (6.32) 6.33 (6.31) 7.68 (7.65) IS Name 13C-PFBA 13C-PFHxA 180-PFHS 13C-PFHxA 13C-PFHxA 180-PFHS 13C-PFDA 13C-PFDA IS Area 4200800 4591100 2619300 4591100 4591100 2619300 2744000 2744000 Analyte Area 63935 41155 71072 73959 27344 91988 2619300 2744000 Exp Cone (ng/mL) 0.10 0.10 0.10 0.10 0.10 0.10 1.00 1.00 Calc. Cone (ng/mL) 0.09 Accuracy {%) 87 0.07 73 0.09 94 0.10 97 0.09 95 0.10 97 1.08 108 1.00 100 Mod? No No No No No No No No k[ 307 Page 13 o f 245 IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA p. 32 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Expected) 1.26(1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.24 (6.22) 6.33 (6.32) 1.26(1.26) 5.31 (5.30) Area 63935 41155 28769 7450 74839 36235 4200800 4591100 Modified? No No No No No No No No Calculated Ion Ratio (Expected Value) 1.00 1.00 0.40 0.10 2.74 0.39 65.70 62.08 308 Page 14 o f 245 p. 33 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review PFC O.IOppb ijfSi c. 0110 j' soon TOGO I PFBA 7 213.0/169.0 Da Q Baseline Missed n Wrnnn Peak littfO 4.26 _ lUOOu >5 4Q 1 *5 SO PFBS 7 299.0/80.0 Da Baseline Missed n Wrnnn Peak tooo 6.^4 sot 8 4CKI t- 1006 * OC'OO 55 cs fs ;o 6? S.S i& 4e5 I ?***5 1^3 05 ic is ;o 13C-PFBA 217.0/172.0 Da C Baseline Missed n Wrnnn Peak b7S & i| 23UetSS? 6.^3 5S 60 65 76 18 0 -P F H S 403.0/103.0 Da Baseline Missed 10*6 e ** 12 <0*5 20* n Wrnnn Peak 5[ 45 50 55 0 5.4 I 3.60 1(1 35 40 >0*6 & 80*5 g 50e5 2 40.6 :o*5 5 45 St ITim*dm>55 (.0 PFPeAl 13C-PFHXA 263.0/219.0 Da 315.0/270.0 Da D Baseline Missed 15006 n Wrnnn Peak 5.31 2*|.& 10600 5000 ___ 45 SO 55 60 c Baseline a Missed n Wrnnn Peak 1*6 I l0*6 & 1 60*6 2 40eS 70.5 45 50 S5 bO PFHxAl 313.0/269.0 Da Baseline Missed n Wrnnn Peak 25000 6.33 ?DOD0 ^ 15000 | 10000 soon 55 60 l 65 70 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak t7o*5! 1 ! 515 7U"a55 55 68 I 65 70 PFHpA~1 13C-PFHxA P F H xil 18 0 -P F H S 363.0/169.0 Da 315.0/270.0 Da 399.0/80.0 Da 403.0/103.0 Da D Baseline a Missed Baseline Missed Baseline Missed Baseline Missed n Wrnnn Peak %4 | u5 ?it%? 5? 00 I 45 70 n Wrnnn Peak 65Ue.5S? 1| 3-955 7.^8 US 70 75Tim*mm 80 n Wrnnn Peak 6?%*5 i& US 1 7UcSS 85 70 75I 80 n Wrnnn Peak 67**55 7.68 i8| *4.*5 2l*55 85 70 75 80 65 S_180-PFHS 13C-PFDA S 13C-PFDA 13C-PFDA 403.0/103.0 Da 515.0/470.0 Da 515.0/470.0 Da 515.0/470.0 Da Baseline Missed Baseline Missed Baseline c Missed Baseline o Missed n Wrnnn Peak_____ n Wrnnn Peak_____ _n Wrnnn. Peak_____ -p Wrona.Peak___ Before After 309 Page 15 of 245 Ion Ratio Confirmation Created with AnalySt importer Printed: 15/02/2010 2:34:00 PM PFC 0.10ppb PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak 000 000 PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak town 45 60 65 70 PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS 2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed a Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak lTis naqe isnof avaiabte N/A Baseline Missed Wrong Peak Page 16 o f 245 310 Services' p. 35 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 0.5ppb 2/9/2010 3:44:57 AM PFCkinetexShort.dam LCMS2-85B Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 3 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA 1 1.26 (1.27) IS Name 13C-PFBA IS Area 4133000 PFPeA 1 3.60 (3.60) 13C-PFHxA 4554300 PFBS 1 4.26 (4.26) 180-PFHS 2687100 PFHxA 1 5.31 (5.31) 13C-PFHxA 4554300 PFHpA 1 6.24 (6.23) 13C-PFHxA 4554300 PFHxS 1 S 180PFHS S 13CPFDA 6.33(6.32) 6.33 (6.31) 7.68 (7.65) 180-PFHS 13C-PFDA 13C-PFDA 2687100 2965500 2965500 Analyte Area 238600 223910 450860 266230 100000 434270 2687100 2965500 Exp Cone (ng/mL) 0.50 0.50 0.50 0.50 0.50 0.50 1.00 1.00 Calc. Cone (ng/mL) 0.49 Accuracy {%) 98 0.49 99 0.49 99 0.49 98 0.51 101 0.48 95 1.03 103 1.00 100 Mod? No No No No No No No No v Page 17 o f 245 311 IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA 2 PFHxS2 S_13C-PFBA S_13C-PFHxA Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Expected) 1.26(1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.23(6.22) 6.33 (6.32) 1.26 (1.26) 5.31 (5.30) Area 238600 223910 167620 24915 350840 165980 4133000 4554300 Modified? No No No No No No No No Calculated Ion Ratio (Expected Value) 1.00 1.00 0.37 0.09 3.51 0.38 17.32 17.11 Page 18 of 245 312 p. 37 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 1.26 PFBA 1 213.0/169.0 Da c Baseline Missed n Wrnnn Peak L 05 Hi 13C-PFBA 217.0/172.0 Da d Baseline Missed i Wrnnn Peak 7* s i" J4 1*4 3.60 1 1. PFC 0.5ppb 5.31 g 60*5 1 0*5 ._ _____1' 0*S 1 PFPe/U 263.0/219.0 Da Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFBS 1 299.0/80.0 Da o Baseline Missed n W rnnn Peak 180-PFHS 403.0/103.0 Da Baseline o Missed n Wrnnn Pp.ak I PFHxAl 313.0/269.0 Da d Baseline o Missed n Wrnnn Peak Trr* nw 130-PFHxA 315.0/270.0 Da d Baseline Missed n Wrnnn Ppak PFHpA 1 363.0/169.0 Da Baseline Missed n Wrnnn Peak 55 t o 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak S_180-PFHS 403.0/103.0 Da o Baseline Missed _____ n W rn nn Poak 13C-PFDA 515.0/470.0 Da Baseline Missed _____ n Wrnnn Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed _____ n W rnnn Pnak Before After 13C-PFDA 515.0/470.0 Da Baseline Missed _____ n Wrnnn Peak Page 19 o f 245 313 Srvk$" p. 38 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 3.60 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline q Missed Wrong Peak PFC 0.5ppb eow to J6e* 06r PFBS 2 299.0/99.0 Da o Baseline a Missed Wrong Peak PFHxA 2 313.0/119.0 Da o Baseline Missed Wrong Peak SS l i6v 3... PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A q Baseline Missed Wrong Peak 314 Page 20 o f 245 p. 39 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 1.0ppb 2/9/2010 3:54:29 AM PFCkinetexShort.dam LCMS2-85C Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 4 5 Standard 1.00 0.00 Results Summary | Analyte | Name Analyte RT (Expected) PFBA1 1.27(1.27) PFPeA 1 3.60 (3.60) PFBS 1 4.26 (4.26) PFHxA 1 5.31 (5.31) PFHpA 1 6.23 (6.23) PFHxS 1 S 180PFHS S 13CPFDA 6.32(6.32) 6.32 (6.31) 7.66 (7.65) IS Name 13C-PFBA 13C-PFHXA 180-PFHS 13C-PFHxA 13C-PFHxA 180-PFHS 13C-PFDA 13C-PFDA IS Area 4089300 4523600 2661400 4523600 4523600 2661400 2843300 2843300 Analyte Area 478990 483470 944980 514540 179220 920280 2661400 2843300 Exp Cone (ng/ml_) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Calc. Cone (ng/ml_) 1.05 Accuracy (%) 105 1.10 110 1.03 1.01 0.96 1.03 103 101 96 103 1.06 106 1.00 100 Mod? No No No No No No No No 315 Page 21 of 245 lombi: IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA 2 PFHpA2 PFHxS2 S__13C-PFBA S_13C-PFHxA p. 40 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Expected) 1.27(1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.23 (6.22) 6.32 (6.32) 1.26 (1.26) 5.31 (5.30) Area 478990 483470 352080 47014 664500 341970 4089300 4523600 Modified? No No No No No No No No Calculated Ion Ratio (Expected Value) 1.00 1.00 0.37 0.09 3.71 0.37 8.54 8.79 Page 22 of 245 316 p. 41 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review PFC 1.0ppb 4 6*4 S. (4 1 4*4 2 "* 7*4 05 I 10 15 PFBA? 6*6 5*5 8 >95 *r | 3*5 2 ?eS 1.5 1.^ r 0 05 70 15 Tim*, mm 13C-PFBA 10*5 0*4 i S0H 1 40*4 20*4 20 3^) 30 35 PFPeAl 40 1 0.6 0 60*5 1 60*5 s 2 40*5 6*6 45 I 45 50 55 13C-PFHXA 6) 213.0/169.0 Da 217.0/172.0 Da 263.0/219.0 Da 315.0/270.0 Da Baseline Missed r , Wrnnn Peak rs.s 20S 1 !Se5 S 10e5 50*4 I 35 40 45 Tin min PFBS 1 50 c Baseline Missed n Wrnnn Peak l#5 2*5 6*5 1 i 4*5 ! *s 2*5 1*5 55 I 5.0 65 70 18 0 - P F H S Baseline Missed n Wrnnn Peak 10*6 a r 2 50*4 1 45 50 55 PFHx a I 60 Baseline Missed n Wrnnn Peak l 2*0 l 0*6 5 .[ I S*5 i S#$ % s 40*5 2*5 45 50 55 63 13C-PFHXA 299.0/80.0 Da 403.0/103.0 Da 313.0/269.0 Da 315.0/270.0 Da Q Baseline Missed n Wrnnn Peak 6*4 6 . ^ 4 '*4 1*4 55 Ul 55 Tima nun PFHpA 1 70 Baseline Missed n Wrnnn Peak 12*6 5,^1 1tefe & * 60e6 5 40*5 reS 45 50 55 60 13C-PFHXA Baseline Missed n Wrnnn Peak 25*5 6.32 20*6 ! | 10*5 66*4 56 60 65 Tint*, mm PFHxS 1 70 Baseline Missed n Wrnnn Peak 60S 7*5 6*6 & 6*5 * 4*5 i- 2*6 1.5 55 I 6.0 65 Tmw. mm 70 18 0 - P F H S 363.0/169.0 Da 315.0/270.0 Da 399.0/80.0 Da 403.0/103.0 Da Baseline Missed M 7*5 M % ** i 4*5 n Wrnnn Peak 6.32 I 2*5 1.5 55 I ID 05 70 S 180-PFHS Baseline Missed Baseline Missed Baseline Missed n Wrnnn Peak n Wrnnn Peak n Wrnnn Peak *5 7*5 6*S & 5*5 | 4*5 S 7*5 2*5 1*5 70 7.^> 75 Tim# m, DO 0*5 t7i*6i $ S*5 1* S 3.5 2*5 1.5 05 70 7.^5 76 60 6*5 7.6 Sai & 5*5 | 4*6 I 3.5 2.5 1.5 5 70 7.66 l 75 Tun*, nun SO 65 13C-PFDA S_13C-PFDA 13C-PFDA 403.0/103.0 Da 515.0/470.0 Da 515.0/470.0 Da 515.0/470.0 Da Baseline Missed n Wrnnn Peak__________ c Baseline Missed n Wrnnn Peak__________ Baseline Missed n Wrnnn Peak_____ Baseline Missed n Wrnnn Peak Before After Page 23 o f 245 317 Services" p. 42 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation T.27 PFC 1.0ppb PFBA2 213.0/169.0 Da Baseline Missed d Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak L PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak S(X ooo PFHpA2 363.0/319.0 Da Baseline Missed o Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da d Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After Fnisrnagei-snofavaisble N/A o Baseline Missed Wrong Peak Page 24 of 245 318 Created with Analyst'(Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 5.0ppb 2/9/2010 4:04:01 AM PFCkinetexShort.dam LCMS2-85D Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 5 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA1 1.27(1.27) IS Name 13C-PFBA IS Area 4014300 PFPeA 1 3.60(3.60) 13C-PFHXA 4518700 PFBS 1 4.26 (4.26) 180-PFHS 2647300 PFHxA 1 5.31 (5.31) 13C-PFHxA 4518700 PFHpA 1 6.22 (6.23) 13C-PFHxA 4518700 PFHxS 1 S 180PFHS S 13CPFDA 6.32(6.32) 6.32(6.31) 7.66 (7.65) 180-PFHS 13C-PFDA 13C-PFDA 2647300 3213800 3213800 Analyte Area 2290700 2382800 4686000 2458900 941350 4379000 2647300 3213800 Exp Cone (ng/mL) 5.00 5.00 5.00 5.00 5.00 5.00 1.00 1.00 Calc. Cone (ng/mL) 5.33 Accuracy (%) 107 5.50 110 5.16 103 5.09 102 5.32 106 5.09 102 0.93 93 1.00 100 Mod? No No No No No No No No 319 4 *1$ (iO Page 25 of 245 IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA p. 44 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Expected) 1.27(1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.22 (6.22) 6.32 (6.32) 1.27(1.26) 5.31 (5.30) Area 2290700 2382800 1791000 225570 2709700 1657900 4014300 4518700 Modified? No No No No No No No No Calculated Ion Ratio (Expected Value) 1.00 1.00 0.38 0.09 2.88 0.38 1.75 1.84 Page 26 of 245 320 Quantitative Peak Review p. 45 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM PFC 5.0ppb PFBA 1 213.0/169.0 Da g Baseline Missed n Wrnnn Peak 13C-PFBA 217.0/172.0 Da Baseline Missed n W rnnn Peak PFPeA 1 263.0/219.0 Da Baseline Missed Wrnnn Peak 13C-PFHxA 315.0/270.0 Da d Baseline Missed n Wrnnn Pnak PFBS 1 299.0/80.0 Da Baseline Missed n W rn n n Pnak 180-PFHS 403.0/103.0 Da Baseline o Missed n Wrnnn Peak PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n W rnnn Pnak PFHpA 1 363 .0/16 9.0 Da Baseline Missed W rnnn Peak 13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Pnak 180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak S_180-PFHS 403.0/103.0 Da o Baseline Missed n W rnnn Peak 13C-PFDA 515.0/470.0 Da c Baseline Missed n Wrnnn Peak___ S_13C-PFDA 515.0/470.0 Da c Baseline Missed ____n W rn nn Peak___ Before After 13C-PFDA 515.0/470.0 Da Baseline Missed ___ n W rnnn.Peak___ Page 27 of 245 321 p. 46 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 1.27 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak PFC 5.0ppb BGfS (Ori OiS OGeCI PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak 6.::2 SS GO OS PFHpA2 363.0/319.0 Da Baseline Missed d Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak SJ3C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 28 of 245 322 p. 47 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC10ppb 2/9/2010 4:13:34 AM PFCkinetexShort.dam LCMS2-85E Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 6 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA1 1.27 (1.27) IS Name 13C-PFBA IS Area 4039300 PFPeA 1 3.60 (3.60) 13C-PFHXA 4527400 PFBS 1 4.26 (4.26) 180-PFHS 2676900 PFHxA 1 5.31 (5.31) 13C-PFHxA 4527400 PFHpA 1 6.22 (6.23) 13C-PFHxA 4527400 PFHxS 1 S 180PFHS S 13CPFDA 6.32 (6.32) 6.31 (6.31) 7.65 (7.65) 180-PFHS 13C-PFDA 13C-PFDA 2676900 2957400 2957400 Analyte Area 4764700 5021600 9581100 5139400 1913100 9153300 2676900 2957400 Exp Cone (ng/mL) 10.00 10.00 10.00 10.00 10.00 10.00 1.00 1.00 Calc. Cone (ng/mL) 11.10 Accuracy (%) 111 11.60 116 10.70 107 10.90 109 10.90 109 10.90 109 1.03 103 1.00 100 Mod? No No No No No No No No Page 29 of 245 323 IRC Summary Analyte Peak Name PFBA 2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA p. 48 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Expected) 1.27(1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.23(6.22) 6.32(6.32) 1.26 (1.26) 5.30 (5.30) Area 4764700 5021600 3769400 466950 5644600 3569800 4039300 4527400 Modified? No No No No No No No No Calculated Ion Ratio (Expected Value) 1.00 1.00 0.39 0.09 2.95 0.39 0.85 0.88 324 Page 30 o f 245 Quantitative Peak Review p. 49 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM PFC 10ppb Before After Page 31 of 245 325 iim bia Services" p. 50 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 3.60 PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak .6:23..... I O"M!.. 18 IBi1l l....4.8.. --11 PFPeA* 2 263.0/219.0 Da Baseline Missed o Wrong Peak 6- - ; PFC 10ppb PFBS2 299.0/99.0 Da d Baseline o Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline o Missed Wrong Peak terigeisiw faiai PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak SJ3C-PFDA 515.0/470.0 Da a Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A a Baseline Missed Wrong Peak Page 32 of 245 326 p. 51 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb 2/9/2010 4:23:09 AM PFCkinetexShort.dam LCMS2-85F Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 7 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA 1 1.26(1.27) PFPeA 1 3.59 (3.60) PFBS 1 4.25 (4.26) PFHxA 1 5.30 (5.31) PFHpA 1 6.22 (6.23) PFHxS 1 S 180PFHS S 13CPFDA 6.32 (6.32) 6.31 (6.31) 7.66 (7.65) IS Name 13C-PFBA 13C-PFHxA 180-PFHS 13C-PFHxA 13C-PFHxA 180-PFHS 13C-PFDA 13C-PFDA IS Area 4227700 4795800 2734400 4795800 4795800 2734400 3316000 3316000 Analyte Area 8381600 8721900 16168000 8985200 3405200 15045000 2734400 3316000 Exp Cone (ng/mL) 20.00 20.00 20.00 20.00 20.00 20.00 1.00 1.00 Calc. Cone (ng/mL) 18.70 Accuracy {%) 93 19.00 95 18.60 93 18.60 93 18.40 92 18.40 92 0.94 94 1.00 100 Mod? No No No No No No No No Page 33 of 245 327 IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA p. 52 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Expected) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.22) 6.31 (6.32) 1.26(1.26) 5.30 (5.30) Area 8381600 8721900 6711000 837810 9866600 6194000 4227700 4795800 Modified? No No No No No No No No Calculated Ion Ratio (Expected Value) 1.00 1.00 0.42 0.09 2.90 0.41 0.50 0.53 328 Page 34 o f 245 lumbia Quantitative Peak Review p. 53 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM PFC 20ppb Before After Page 35 of 245 329 Ion Ratio Confirmation p. 54 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM PFC 20ppb PFBA2 213.0/169.0 Da u Baseline Missed Wrong Peak J2 2 - PFPeA2 263.0/219.0 Da Baseline Missed a Wrong Peak 6; 1 PFBS2 299.0/99.0 Da d Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Ttetnageisndavaiate PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 36 of 245 330 p. 55 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM te Project Data File Acquisition Method Ewan's Projects\PFCs 02081 OVCalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 50ppb 2/9/2010 4:32:41 AM PFCkinetexShort.dam LCMS2-85G Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 8 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA 1 1.27 (1.27) PFPeA 1 3.59(3.60) PFBS 1 4.25 (4.26) PFHxA 1 5.30 (5.31) PFHpA 1 6.23 (6.23) PFHxS 1 S 180PFHS S 13CPFDA 6.32 (6.32) 6.33(6.31) 7.67 (7.65) IS Name 13C-PFBA 13C-PFHxA 180-PFHS 13C-PFHxA 13C-PFHxA 180-PFHS 13C-PFDA 13C-PFDA IS Area 4234600 4753100 2676100 4753100 4753100 2676100 3257900 3257900 Analyte Area 22387000 22184000 34874000 21291000 9090100 31836000 2676100 3257900 Exp Cone (ng/mL) 50.00 50.00 50.00 50.00 50.00 50.00 1.00 1.00 Calc. Cone (ng/mL) 49.90 Accuracy (%) 100 48.80 98 50.70 101 50.50 101 50.40 101 50.80 102 0.93 93 1.00 100 Mod? No No No No No No No No Page 37 o f 245 331 IRC Summary I Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA p. 56 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Expected) 1.27(1.27) 3.59 (3.60) 4.26 (4.26) 5.31 (5.31) 6.24 (6.22) 6.33 (6.32) 1.27(1.26) 5.31 (5.30) Area 22387000 22184000 16796000 2281900 22816000 15495000 4234600 4753100 Modified? No No No No No No No No Calculated Ion Ratio (Expected Value) 1.00 1.00 0.48 0.11 2.51 0.49 0.19 0.22 Page 38 of 245 332 p. 57 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review PFC 50ppb ! j* i 5*5 & 4*5 < i3 Je5 1*5 OS i 10 is 4*5 1 JI i*4 Z0 3^ )0 56 40 !*L 10*6 80*5 i fid*5 5.31 1 40* J0*5 ________ i 45 46 50 56 50 PFBAl" 213.0/169.0 Da c Baseline Missed n Wrnnn Peak 4.^ 6*f 2S M )5 40 45 50 pfbsT 299.0/80.0 Da o Baseline Missed n Wrnnn Peak i f w I 4 0 C5 Ml 13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak 8e5 6.^3 7*5 6*5 3 4*5 I* lei. 55 b.O 65 Tun*. mi 180-PFHS 78 403.0/103.0 Da Baseline Missed n Wrnnn Peak 5.^1 10*4 80*5 f i 4&5 45 50 55 50 PFPe/U 263.0/219.0 Da Baseline Missed n Wrnnn Peak 40*6 70.6 s1 20*6 10*6 I 45 50 55 Tn* r* i PFHxA 1 50 313.0/269.0 Da C Baseline Missed n Wrnnn Peak 7*6 6.32 6*8 " v 1M 2 ;-*c 1*6 55 60 65 70 13C-PFHxA 315.0/270.0 Da c Baseline Missed n Wrnnn Peak 1Z*6 5.31 10*6 | 80*S | 60*5 f 40*5 :o*s 45 1 6 ii 55 6-5 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak to* 7*5 6e5 & 5*5 i * 1 7*5 1*5 66 80 05 7U PFHp/Ti 363.0/169.0 Da d Baseline Missed _______ n Wronn Peak_____ 8*5 6.33 7*5 $6 < .**5 I:5 le5 $5 l 6 86 & iI s 70 S 180-PFHS 403.0/103.0 Da Q Baseline Missed p Wrnnn Peak_____ 13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak 6*5 1 4*5 $ 2 7e5 I 7(i 75 86 Tn*. mm 13C-PFDA IS 515.0/470.0 Da c Baseline Missed p Wrnnn Peak_____ PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak 7.67 85 6.6 4*5 C*5 70 l 75 <0 8 * $ 2 85 S 13C-PFDA 515.0/470.0 Da c Baseline Missed n Wrnnn Peak_____ 180-PFHS 403.0/103.0 Da Baseline o Missed n Wrnnn Peak 7.^7 8*6 5*5 4*5 Z5 70 75 BO 65 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak__ Before After Page 39 of 245 333 Ion Ratio Confirmation 1727" 3759' p. 58 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM PFC 50ppb PFBA2 213.0/169.0 Da c Baseline d Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline a Missed Wrong Peak PFBS2 299.0/99.0 Da Baseline q Missed a Wrong Peak PFHxA 2 313.0/119.0 Da d Baseline Missed Wrong Peak PFHpA2 363.0/319.0 Da o Baseline Missed o Wrong Peak PFHxS2 399.0/99.0 Da a Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A d Baseline Missed d Wrong Peak Page 40 of 245 334 im s ' Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb ICV 2/9/2010 5:01:26 AM PFCkinetexShort.dam LCMS2-77J Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 9 5 Quality Control 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA 1 1.27 (1.27) PFPeA 1 3.61 (3.60) PFBS 1 4.27 (4.26) PFHxA 1 5.32(5.31) PFHpA 1 6.24 (6.23) PFHxS 1 S 180PFHS S 13CPFDA 6.34 (6.32) 6.34(6.31) 7.68 (7.65) IS Name 13C-PFBA 13C-PFHxA 180-PFHS 13C-PFHxA 13C-PFHxA 180-PFHS 13C-PFDA 13C-PFDA IS Area 4090400 4533900 2648500 4533900 4533900 2648500 3123300 3123300 Analyte Area 9226300 9681900 17751000 9358200 3733600 16240000 2648500 3123300 Exp Cone (ng/mL) 20.00 20.00 20.00 20.00 20.00 20.00 1.00 1.00 Calc, Cone (ng/mL) 21.30 Accuracy j (%) | wloa 106 No 22.30 112 No 21.40 107 No 20.60 103 No 21.40 107 No 20.90 104 No 0.96 96 No 1.00 100 No IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 SJ3C-PFBA S_13C-PFHxA p. 60 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT {Exp RT) 1.27(1.27) 3.61 (3.60) 4.26 (4.26) 5.32 (5.31) 6.25 (6.22) 6.34 (6.32) 1.27(1.26) 5.32(5.30) Area 9226300 9681900 7371700 939420 10767000 6702600 4090400 4533900 Modified? No No No No No No No No Ratio Confirms ID YES YES YES YES YES YES No No 336 Page 46 of 245 Quantitative Peak Review p. 61 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM PFC 20ppb ICV Before After 337 Page 47 of 245 p. 62 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 3.61 Ur PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak -6^5- PFPeA2 263.0/219.0 Da Baseline Missed d Wrong Peak PFC 20ppb ICV Tim m*n PFBS 2 299.0/99.0 Da Baseline Missed Wrong Peak S OfeS 00.il PFHxA 2 313.0/119.0 Da o Baseline Missed Wrong Peak PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da c Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da d Baseline Missed a Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed o Wrong Peak 338 Page 48 of 245 Created with Analys/pF^porter Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: Data File Acquisition Date Acquisition Method Project PFOA 1 13C-PFOA 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's ProjectsVPFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Regression Equation:________ y = 0.0508 x + -0.00146 (r = 0.9989)7 Exp Cone (ng/mL) # of Values Mean Calc Cone (ng/mL) 0.1 1 0.114 0.5 1 0.477 1 1 0.958 5 1 4.871 10 1 10.158 20 1 18.413 50 1 51.608 % Accuracy 114 95 96 97 102 92 103 STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN ,,,,Li n >4 r . i t f a :> ?> ; t a ?s > y , y r o v x it i? O 44 4t 47 # 4 Page 1 of 170 339 Km v Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: PFNA 1 13C-PFNA Data File Acquisition Date Acquisition Method Project 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 y = 0.109 x + -0,00371 (r= 0.9976) >/ # of Values Mean Calc Cone (ng/mL) 1 0.107 1 0.463 1 1.096 1 4.964 1 9.849 1 17.699 1 52.422 % Accuracy 107 93 110 99 98 88 105 / STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN 1 _______ C 3 - * %< ' 6 i6 i? h n ;n : i ? : z i z* j* r :e n AnaM* Cone /IS Cone w i iz 3) * Page 2 o f 170 340 Created with AnalysPFfjborter Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: PFOS 1 13C-PFOS Data File Acquisition Date Acquisition Method Project 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 02171 Olong.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 y = 0.107 x + -0.0016 (r = 0.9989) ^ # of Values Mean Calc Cone (ng/mL) 1 0.093 1 0.489 1 1.026 1 5.187 1 10.889 1 18.688 1 50.229 % Accuracy 93 98 103 104 109 93 100 STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN o< 6. ' tf ) i; II If I] H IS 1s f3 i* K 26 7 28 71 ill 3l 32 1* K )( 1' 39 :* <0 n V v* ^7 Page 3 of 170 341 p. 66 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: PFDA 1 13C-PFDA Data File Acquisition Date Acquisition Method Project Regression Equation: Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 y = 0.103 x + 0.000852 (r = 0.9990) / # of Values Mean Calc Cone (ng/mL) 1 0.099 1 0.482 1 1.103 1 4.925 1 10.077 1 18.489 1 51.425 % Accuracy 99 96 110 99 101 92 103 STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN 342 Page 4 of 170 Hi ( l lurnbia Created with A naiyiV Importer Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: PFUnA 1 13C-PFUnA Data File Acquisition Date Acquisition Method Project 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Regression Equation:________ y = 1.05 x + 0.0336 (r = 0.9994) ^ Exp Cone (ng/mL) # of Values Mean Calc Cone (ng/mL) 0.1 1 0.083 0.5 1 0.530 1 1 1.090 5 1 . 5.374 10 1 9.317 20 1 20.372 50 1 49.833 % Accuracy 83 106 109 107 93 102 100 y STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN ' 6 ' 3 *> 1C- ;% 'A, v & z* xi 3' n i- K % 3/ n r> *t- w < as * a 4` h -A Page 5 o f 170 343 Created witn Anals^fteporter Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: PFDS 1 13C-PFOS Data File Acquisition Date Acquisition Method Project 02171 Q\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/mL) 0.1 0.5 1 5 10 20 50 y = -0.000414 xA2 + 0.118 x + -0,00244 (r = 0.9991) / # of Values Mean Calc Cone (ng/mL) % Accuracy 1 0.095 95 1 0.499 100 1 1.019 102 1 5.061 101 1 10.781 108 1 18.757 94 1 50.430 101 / STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN -::i A'' 29 X 31 32 33 34 35 3t 45 46 47 m 4S 4 HV T\v 2^1 Page 6 o f 170 Created with AnalysTReporter Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: PFDoA 1 13C-PFDoA .Data File Acquisition Date Acquisition Method Project 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/ml_) 0.1 0.5 1 5 10 20 50 y = 0.605 x + -0.00456 (r = 0.9959) 'J # of Values Mean Calc Cone (ng/mL) 1 0.089 1 0.563 1 0.825 1 5.565 1 9.667 1 22.817 1 47.074 % Accuracy 89 113 83 111 97 114 94 STD NaN NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN NaN \-> H M H I? IB IS i t :s it, v AnM '.one /IS Cone : so 3i j:' jj h is * s n i c -n u r . * o <* 345 '>1 2^./ i ( Page 7 o f 170 p. 70 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Analyte Name: Internal Standard: PFTrA 1 13C-PFDoA Data File Acquisition Date Acquisition Method Project 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/mL) 0.5 1 5 10 20 50 y = 0.336 x + 0.0691 (r= 0.9959) 7 # of Values Mean Calc Cone (ng/mL) 1 0.456 1 0.800 1 5.804 1 11.171 1 21.489 1 46.780 % Accuracy 91 80 116 112 107 94 / STD NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN ^ ; ) j i b ; e 9 to *i u h is ir u '9 :i 2? j i 24 k a :? 2s n m 3i k 33 34 3s x v 3? 39 *> -n c <3 4* <> no** Cone ,'/$ Cone tC * ^ 7 . , ( t *< lG Page 8 of 170 346 Createci with A nalysP F ^orter Printed: 18/02/2010 3:24:00 PM w Analyte Name: Internal Standard: PFTeA 1 13C-PFDoA Data File Acquisition Date Acquisition Method Project 021710\CalLong.wiff 2/17/2010 6:30:13 PM PFCkinetexLong.dam Ewan's Projects\PFCs Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Regression Equation: Exp Cone (ng/mL) 0.5 1 5 10 20 50 y = 0,000351 xA2 + 0.246 x + 0.0269 (r = 0.9993) ^ # of Values Mean Calc Cone (ng/mL) % Accuracy 1 0.490 98 1 0.931 93 1 5.574 111 1 10.100 101 1 19.165 96 1 50.241 100 STD NaN NaN NaN NaN NaN NaN %CV NaN NaN NaN NaN NaN NaN l i I* U I i i jo :> :r : i 2* n :e : j AnaiyttCone 'ISC'Onc ?s ji : : j ? jr. >( v Page 9 o f 170 347 Created with An aijysf Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Result Table Instrument Name Ewan's Projects\PFCs 021710\CalLong.wiff 021710long.rdb API 5000 Sample Name Acquisition Date Acquisition Method Injection Vial IB 2/17/2010 6:30:13 PM PFCkinetexLong.dam 1 Injection Volume Sample Type Dilution Factor Weight to Volume 5 Unknown 1.00 0.00 Results Summary Analyte Peak Name IS Name PFOA 1 13C-PFOA PFNA 1 13C-PFNA PFOS 1 13C-PFOS PFDA 1 13C-PFDA PFUnA 1 13C-PFUnA PFDS 1 13C-PFOS PFDoA 1 13C-PFD0A PFTrA 1 13C-PFDOA PFTeA 1 13C-PFDOA IS Area 186 185 1700 451 33 1700 174 174 174 Analyte RT (Exp RT ) 1.57 (1.36) 0.00 (2.06) 2.10 (2.09) 0.00(3.06) 0.00 (4.06) 3.94 (3.87) 0.00 (4.55) 5.09 (5.06) 5.42 (5.51) Analyte Area 2795 0 458 0 0 1475 0 4776 4625 Calc. Cone (ng/mL) 296.000 N/A 2.530 N/A N/A 7.550 N/A 81.200 94.900 Modified? No No No No No No No No No 348 T ' i 1 ?l< Page 10 o f 170 Createci with AnalysJ?fTe|)orter Printed: 18/02/2010 3:24:00 PM IRC Summary Analyte P e a k N a m e PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Exp RT ) 0.00(1.36) 1.37 (1.36) 1.90 (2.08) 2.52 (2.44) 2.21 (2.12) 2.20 (2.00) 3.23 (3.06) 3.10 (3.04) 0.00 (3.88) 4.01 (3.88) 0.00 (3.87) 0.00 (4.53) 4.70 (4.64) 5.09 (5.13) 5.52 (5.56) Area 0 186 366 276 155 1700 349 451 0 33 0 0 174 426 179 M odified? No Calc (Exp) MRM Ratio 0.00 (2.16) Ratio Confirms ID No No 0.07 (43.19) No No 0.00 (0.34) No No 0.00 (0.00) No No 0.34(0.32) YES No 3.71 (21.71) No No 0.00 (0.14) No No 0.00(17.53) No No 0.00 (0.19) No No 0.00(1.60) No No 0.00 (0.32) No No 0.00 (0.28) No No 0.00(3.71) No No 0.09 (0.34) No No 0.04 (0.29) No / ( *! f0 Page 11 o f 170 349 ................................................. Columbia O A n alytical Services" Quantitative Peak Review p. 74 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM IB 1 .5 7 JjjWk*iA- / PFOA 1 413.0/169.0 Da g Baseline q Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline o Missed _____ n Wrnnn Peak___ j ., -v./V/v' PFNA 1 463.0/419.0 Da Baseline c Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak 2.10 PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak ., h(\ h , ,/iAA'AAM/r ' * PFUnA 1 563.0/519.0 Da Baseline Missed ___n Wrnnn Peak 13C-PFOS 503.0/80.0 Da o Baseline d Missed n Wrnnn Peak PFDA 1 513.0/469.0 Da d Baseline Missed n Wrnnn Peak ,ih ' | 4 .0 1 r e [` ,M I! i !l f i t ii in' i---------( i n ---------------------------------f r 5 lOOOu *OOU coo 1000 3 .9 4 13C-PFUnA 565.0/270.0 Da Baseline Missed ___n Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak too 500 0 & a 3oo i 1 7" ) r 13C-PFOS 503.0/80.0 Da Baseline Missed n W rnnn Peak 4 .7 0 <U 45 Tima mtfi PFDoA 1 50 613.0/569.0 Da Baseline Missed n Wrnnn Peak <m 5 .4 2 , , \> , i a il. \A i, , ; iVa' V'Alj' Ioi.,JUj\*51-A,mV '4 JJyjMV j-;i 13C-PFD0A 615.0/169.0 Da Baseline a Missed r Wrnnn Peak 4 .7 0 PFTrA 1 663.0/619.0 Da Baseline Missed ___ n Wrnnn Peak___ PFTeA 1 713.0/669.0 Da Baseline a Missed n Wrnnn Peak ,, k A / aI ^ V 1 i_____L___ 13C-PFDoA 615.0/169.0 Da c Baseline Missed n Wrnnn Peak Before After 13C-PFD0A 615.0/169.0 Da d Baseline o Missed _____ n W rnnn Peak Page 12 of 170 350 lu m b ta Ion Ratio Confirmation Created with A nalyi?'$fporter Printed: 18/02/2010 3:24:00 PM IB PFOA2 413.0/369.0 Da o Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak Wt'< hA l\ . hh A / y uJnlv' 1000f IV 'ooon ,, 1000 iil mo 4000 5!. 40 4S PFUnA 2 563.0/269.0 Da Baseline Missed Wrong Peak jo 40 4$ PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da Baseline o Missed c Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak l . .fiiJ , A I. 8 'nA m w k f k m i 5r `-ot 1 * 1000 X4l WO 40 a jo PFDoA2 613.0/169.0 Da Baseline Missed d Wrong Peak PFTrA2 663.0/169.0 Da Baseline Misse Wrong Peak y 5.52:, PFTeA2 713.0/169.0 Da d Baseline Missed Wrong Peak Before After Page 13 o f 170 351 p. 76 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 021710\CalLong.wiff PFCkinetexLong.dam Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 0.10ppb 2/17/2010 6:52:57 PM PFCkinetexLong.dam LCMS2-85A Results Summary Analyte Name Analyte RT (Expected) PFOA1 1.23(1.36) IS Name 13C-PFOA IS Area 3289300 PFNA 1 2.06 (2.06) 13C-PFNA 2669800 PFOS 1 2.10(2.09) 13C-PFOS 7592300 PFDA 1 3.10(3.06) 13C-PFDA 2504200 PFUnA 1 3.92(4.06) 13C-PFUnA 222150 PFDS 1 3.91 (3.87) 13C-PFOS 7592300 PFDoA 1 4.54 (4.55) 13C-PFDOA 305390 PFTrA 1 5.07 (5.06) 13C-PFDOA 305390 PFTeA 1 5.56 (5.51) 13C-PFDOA 305390 Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 2 5 Standard 1.00 0.00 Analyte Area 14318 21049 63477 27648 26665 66470 14964 10486 6622 Exp Cone (ng/mL) 0.100 0.100 0.100 0.100 0.100 0.100 0.100 0.100 0.100 Calc. Cone (ng/mL) 0.114 Accuracy (%) 114 0.107 107 0.093 93 0.099 99 0.083 83 0.095 95 0.089 89 N/A N/A N/A N/A Mod? No No No No No No No No No Page 14 o f 170 352 m wtK& IRC Summary I Analyte Peak Name PFOA 2 S_13C_PFOA PFIMA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 Created with A n a ly it Reporter Printed: 18/02/2010 3:24:00 PM RT (Expected) 1.25 (1.36) 1.24(1.36) 2.08 (2.08) 0.00 (2.44) 2.07 (2.12) 2.11 (2.00) 3.12(3.06) 3.12(3.04) 3.89(3.88) 3.92(3.88) 3.91 (3.87) 4.54 (4.53) 4.54 (4.64) 5.11 (5.13) 5.57 (5.56) Area 19544 3289300 8617 0 19074 7592300 3350 2504200 3604 222150 22732 4509 305390 2650 413 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 1.36 229.73 0.41 0.00 0.30 119.61 0.12 90.57 0.14 8.33 0.34 0.30 20.41 0.25 0.06 Page 15 o f 170 353 lytcai ices" Quantitative Peak Review PFOA 1 413.0/169.0 Da d Baseline Missed n Wronn Peak 16 is 13C-PFOA 417.0/372.0 Da d Baseline a Missed ____n Wrnnn Peak___ Created with Anal^st^feporter Printed: 18/02/2010 3:24:00 PM PFC O.IOppb 2.06 tJW /V V i PFNA 1 463.0/419.0 Da Baseline o Missed n Wrnnn Ppak 13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak 3.12 PFOS 1 499.0/80.0 Da Baseline Missed r; W rnnn Ppak 13C-PFOS 503.0/80.0 Da o Baseline Missed n Wrnnn Ppak PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak 3.91 i 13C-PFDA 515.0/470.0 Da Baseline d Missed n Wrnnn PFUnA 1 563.0/519.0 Da Baseline Missed r Wrnnn Peak 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wronn Peak Tim mm PFDS 1 599.0/80.0 Da Baseline a Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak PFDoA 1 613.0/569.0 Da d Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak 5.07 0 0*0 PFTrA'l 663.0/619.0 Da Baseline Missed n W rnnn Peak___ 13C-PFDoA 615.0/169.0 Da Baseline Missed n W rnnn P e ak___ 5.56 PFTeA 1 713.0/669.0 Da Baseline Missed n W rnnn Peak 13C-PFDOA 615.0/169.0 Da c Baseline Missed ___ n W ronn Ppak___ Before After Page 16 of 170 354 Created with Analyst ^ p o r te r Printed: 18/02/2010 3 24:00 PM Ion Ratio Confirmation PFC 0.10ppb 1.25 PFOA2 413.0/369.0 Da Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da g Baseline Missed Wrong Peak PFUnA 2 563.0/269.0 Da Baseline Missed Wrong Peak PFDS 2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline g Missed Wrong Peak PFTeA 2 713.0/169.0 Da Baseline Missed Wrong Peak Before After 355 Page 17 o f 170 rvKar Created with A nal^st^eporter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 021710\CalLong.wiff PFCkinetexLong.dam Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 0.5ppb 2/17/2010 7:00:32 PM PFCkinetexLong.dam LCMS2-85B Results Summary Analyte Name A nalyte RT (Expected) PFOA 1 1.27(1.36) IS Name 13C-PFOA IS Area 3385400 PFNA 1 2.06 (2.06) 13C-PFNA 2759300 PFOS 1 2.09 (2.09) 13C-PFOS 7581500 PFDA 1 3.10(3.06) 13C-PFDA 2783700 PFUnA 1 3.89 (4.06) 13C-PFUnA 204480 PFDS 1 3.90 (3.87) 13C-PFOS 7581500 PFDoA 1 4.54 (4.55) 13C-PFDOA 275140 PFTrA 1 5.07 (5.06) 13C-PFDOA 275140 PFTeA 1 5.53 (5.51) 13C-PFDOA 275140 Result Table Algorithm Used Instrument Name Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 021710long.rdb IntelliQuan API 5000 3 5 Standard 1.00 0.00 Analyte Area 77019 128820 383910 140780 120250 428430 92483 61214 40588 Exp Cone (ng/mL) 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 Calc. Cone (ng/mL) 0.477 Accuracy (%) 95 0.463 93 0.489 98 0.482 96 0.530 106 0.499 100 0.563 113 0.456 91 0.490 98 Mod? No No No No No No No No No (/ &i ( Page 18 of 170 356 IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFIMA 2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 SJ3C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 Created with A n a ly if Reporter Printed: 18/02/2010 3:24:00 PM RT (Expected) 1.28(1.36) 1.27(1.36) 2.08 (2.08) 0.00 (2.44) 2.13 (2.12) 2.10(2.00) 3.10(3.06) 3.08 (3.04) 3.90 (3.88) 3.92(3.88) 3.90 (3.87) 4.55 (4.53) 4.53 (4.64) 5.08 (5.13) 5.52 (5.56) Area 173300 3385400 42381 0 121730 7581500 18842 2783700 26323 204480 128520 22676 275140 21930 12493 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.25 43.96 0.33 0.00 0.32 19.75 0.13 19.77 0.22 1.70 0.30 0.25 2.98 0.36 0.31 Page 19 o f 170 357 Services* Quantitative Peak Review Created with A nal^st^eporter Printed: 18/02/2010 3:24:00 PM PFC 0.5ppb PFOA 1 413.0/169.0 Da Baseline Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed Wrnnn Peak PFNA 1 463.0/419.0 Da Baseline o Missed n W rnnn Peak 13C-PFNA 468.0/423.0 Da Baseline d Missed n Wrnnn Peak Tim min PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n W rnnn Pp.ak PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Pnak 13C-PFDA 515.0/470.0 Da Baseline a Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da Baseline Missed n W rnnn Peak (` OHO 'AQ0Q 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n W rnnn Peak PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da d Baseline Missed n W rnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed _____ n W rnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n W rnnn Pp.ak___ PFTeA 1 713.0/669.0 Da Baseline Missed n Wrnnn Pnak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak Before After 358 Page 20 o f 170 Ion Ratio Confirmation Created with Analyst ^ p o rte r Printed: 18/02/2010 3:24:00 PM PFC 0.5ppb Before After Page 21 o f 170 359 p. 84 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's ProjectsVPFCs 021710\CalLong.wiff PFCkinetexLong.dam Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 1.0ppb 2/17/2010 7:08:07 PM PFCkinetexLong.dam LCMS2-85C Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.23(1.36) IS Name 13C-PFOA IS Area 3356000 PFNA 1 2.03(2.06) 13C-PFNA 2574400 PFOS 1 2.08 (2.09) 13C-PFOS 7600400 PFDA1 3.08 (3.06) 13C-PFDA 2542500 PFUnA 1 3.90 (4.06) 13C-PFUnA 209310 PFDS 1 3.89 (3.87) 13C-PFOS 7600400 PFDoA 1 4.54 (4.55) 13C-PFDOA 290240 PFTrA 1 5.07 (5.06) 13C-PFDOA 290240 PFTeA 1 5.53 (5.51) 13C-PFDOA 290240 Result Table Algorithm Used Instrument Name Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 021710long.rdb IntelliQuan API 5000 4 5 Standard 1.00 0.00 Analyte Area 158340 297510 821270 291450 245580 893930 143490 98161 74342 Exp Cone (ng/mL) 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 Calc. Cone (ng/mL) 0.958 Accuracy (%) 96 1.100 110 1.030 103 1.100 110 1.090 109 1.020 102 0.825 83 0.800 80 0.931 93 Mod? No No No No No No No No No 360 * (0 7 {< # Page 22 o f 170 Analytical Services' IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Expected) 1.26(1.36) 1.25(1.36) 2.02 (2.08) 0.00 (2.44) 2.07 (2.12) 2.08 (2.00) 3.10 (3.06) 3.05 (3.04) 3.90 (3.88) 3.89 (3.88) 3.89 (3.87) 4.53 (4.53) 4.54 (4.64) 5.07 (5.13) 5.52 (5.56) p. 85 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Area 372000 3356000 93995 0 262120 7600400 36921 2542500 48498 209310 271790 43135 290240 34820 20771 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.35 21.19 0.32 0.00 0.32 9.25 0.13 8.72 0.20 0.85 0.30 0.30 2.02 0.35 0.28 Page 23 o f 170 361 Eumbia Servie ' Quantitative Peak Review ,L PFOA 1 413.0/169.0 Da c Baseline Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak I 2.08 p. 86 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM PFCl.Oppb 2.01 i PFNA 1 463.0/419.0 Da c Baseline Missed n Wrnnn Peak 13C-PFNA 468.0/423.0 Da Baseline Missed _____ n Wrnnn Peak 3.05 PFOS 1 499.0/80.0 Da Baseline Missed ~ W rnnn Peak 1 13C-PFOS 503.0/80.0 Da g Baseline Missed n Wrnnn Paak 10 35 PFDA 1 513.0/469.0 Da d Baseline Missed n Wrnnn Paak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Ppak PFUnA 1 563.0/519.0 Da Baseline Missed r Wrnnn Peak PFDoA 1 613.0/569.0 Da d Baseline Missed Wrnnn Ppak 13C-PFUnA 565.0/270.0 Da Baseline Missed n W rnnn Peak 13C-PFDOA 615.0/169.0 Da d Baseline Missed n Wrnnn Peak PFDS 1 599.0/80.0 Da Baseline Missed i Wrnnn Peak liOQO mom <3000 PFTrA 1 663.0/619.0 Da Baseline Missed r Wrnnn Peak lJ L 13C-PFOS 503.0/80.0 Da Baseline Missed ___r WrnnnPrbK___ 13C-PFDOA 615.0/169.0 Da Baseline Missed _____ n Wrnnn Peak___ PFTeA 1 713.0/669.0 Da Baseline c Missed n W rnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline d Missed n Wrnnn Peak Before After 362 Page 24 of 170 /"i C o liin ib is t O A n alytical Services" Ion Ratio Confirmation Created with A n a ly ^'fflp o rte r Printed: 18/02/2010 3:24:00 PM PFC 1.0ppb PFOA2 413.0/369.0 Da Baseline g Missed Wrong Peak PFUnA2 563.0/269.0 Da d Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline o Missed Wrong Peak 1 1 1 ....... -..."sjsjp.......-..... to )s a Tun win PFDS 2 599.0/99.0 Da Baseline Missed d Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak ; cm :54 g. i 154 ........ 5 1CM 4.p 3......... .... i ... " " 0 45 50 PFDoA 2 613.0/169.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak .' 5000 20000 I '0000 5000 PFTrA 2 663.0/169.0 Da Baseline Misse Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 25 of 170 363 p. 88 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 021710\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 02171 Olong.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 5.0ppb 2/17/2010 7:15:39 PM PFCkinetexLong.dam LCMS2-85D Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 5 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.20 (1.36) IS Name 13C-PFOA IS Area 3384000 PFNA 1 1.98 (2.06) 13C-PFNA 2759300 PFOS 1 2.03 (2.09) 13C-PFOS 7552200 PFDA1 3.06(3.06) 13C-PFDA 2677400 PFUnA 1 3.89 (4.06) 13C-PFUnA 210740 PFDS 1 3.88(3.87) 13C-PFOS 7552200 PFDoA 1 4.52 (4.55) 13C-PFDOA 243760 PFTrA 1 5.06 (5.06) 13C-PFDoA 243760 PFTeA 1 5.51 (5.51) 13C-PFDOA 243760 Analyte Area 831690 1480000 4176000 1362800 1190900 4421900 819010 492610 343170 Exp Cone (ng/mL) 5.000 5.000 5.000 5.000 5.000 5.000 5.000 5.000 5.000 Calc. Cone (ng/mL) 4.870 Accuracy (%) 97 4.960 99 5.190 104 4.930 99 5.370 107 5.060 101 5.560 111 5.800 116 5.570 111 Mod? No No No No No No No No No 364 no Page 26 of 170 IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 p. 89 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM RT (Expected) 1.21 (1.36) 1.19 (1.36) 1.97(2.08) 0.00 (2.44) 2.03 (2.12) 2.01 (2.00) 3.03 (3.06) 3.06 (3.04) 3.86 (3.88) 3.88 (3.88) 3.87 (3.87) 4.53 (4.53) 4.55 (4.64) 5.05 (5.13) 5.51 (5.56) Area 1906300 3384000 478680 0 1325400 7552200 184660 2677400 248160 210740 1386100 232560 243760 160530 126530 M o d ifie d ? No No No No No No No No No No No No No No No C a lc u la te d Ion Ratio (Expected Value) 2.29 4.07 0.32 0.00 0.32 1.81 0.14 1.96 0.21 0.18 0.31 0.28 0.30 0.33 0.37 Page 27 o f 170 365 p. 90 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review PFC 5.0ppb 1i } I Sk 05 lu 1S ?rj PFOA1 413.0/169.0 Da c Baseline Missed n W rnnn Peak L 25*5 i? S 5*5 I so- 05 1.19 k 1 JL >0 15 13C-PFOA 417.0/372.0 Da Baseline Missed n W rnnn Peak 2.01 k 1.98 U1 1 i -1 1 d**' it- lb 5 PFNA 1 463.0/419.0 Da Baseline Missed n W rnnn Peak 3^6 :o*5 ta i^ s SO' 10 13C-PFNA 468.0/423.0 Da Baseline Misse n Wrnnn Peak _JL PFOS 1 499.0/80.0 Da o Baseline Missed n Wrnnn Peak _J }'* <0 L 45 PFUnA 1 563.0/519.0 Da Baseline Missed ___n Wrnnn Peak___ 13C-PFOS 503.0/80.0 Da Baseline Missed PFDA 1 513.0/469.0 Da a Baseline Missed |3.88 .S & sb5 * <** !M ;*s if (3.88 35 *b J5 13C-PFUnA 565.0/270.0 Da Baseline Missed Wrnnn Peak Su 30 35 0 Tunu. mm PFDS 1 45 599.0/80.0 Da c Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da o Baseline Missed 5*5 4*5 lM 1*5 L2 .0 1 13 C -P F O S 503.0/80.0 Da Baseline Missed n Wrnnn Peak PFDoA 1 613.0/569.0 Da Baseline o Missed n Wrnnn Peak 5.51 13C-PFDOA 615.0/169.0 Da d Baseline o Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da d Baseline Missed ____n W rnnn Peak___ 13C-PFD0A 615.0/169.0 Da d Baseline Missed n W rnnn P e ak___ PFTeA 1 713.0/669.0 Da Baseline Missed n W rnnn Peak___ 13C-PFD0A 615.0/169.0 Da Baseline Missed ___ n W rnnn Peak___ Before After 366 Page 28 of 170 p. 91 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Ion Ratio Confirmation ' Set, l { ' 0 t* JL _ PFOA2 413.0/369.0 Da C Baseline Missed Wrong Peak A0c 0*4 | - 1 J 9 1....................... ........... .................... 20*4 IS 2D : s JO PFNA2 463.0/219.0 Da Baseline 0 Missed Wrong Peak PFC 5.0ppb A2S*S rose X ................................... l s*s 6 i o-s ...../ Jl V ' ' n :s jo PFOS2 499.0/99.0 Da Baseline Missed 0 Wrong Peak PFDA2 513.0/269.0 Da c Baseline Misse Wrong Peak PFUnA 2 563.0/269.0 Da Baseline Missed o Wrong Peak PFDS2 599.0/99.0 Da o Baseline Missed o Wrong Peak ............ 4. 93......- ..... r .............. PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak See SM 1 ..............5., 5._............ 0t0u _ _ _ 1 _______ PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 29 o f 170 367 p. 92 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 021710\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 10ppb 2/17/2010 7:23:15 PM PFCkinetexLong.dam LCMS2-85E Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 6 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA1 1.27 (1.36) IS Name 13C-PFOA IS Area 3499200 PFNA 1 2.03(2.06) 13C-PFNA 2760800 PFOS 1 2.09 (2.09) 13C-PFOS 7587400 PFDA 1 3.08 (3.06) 13C-PFDA 2754900 PFUnA 1 3.88 (4.06) 13C-PFUnA 239570 PFDS 1 3.90 (3.87) 13C-PFOS 7587400 PFDoA 1 4.55 (4.55) 13C-PFDoA 291580 PFTrA 1 5.06 (5.06) 13C-PFDOA 291580 PFTeA 1 5.53 (5.51) 13C-PFDOA 291580 Analyte Area 1798800 2948100 8820800 2866500 2341000 9289900 1702900 1115500 742120 Exp Cone (ng/mL) 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 Calc. Cone (ng/mL) 10.200 Accuracy (%) 102 9.850 99 10.900 109 10.100 101 9.320 93 10.800 108 9.670 97 11.200 112 10.100 101 Mod? No No No No No No No No No 368 ~v\V t - H $ l, Page 30 of 170 IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA 2 Created with AnaiysIP-F&fcorter Printed: 18/02/2010 3:24:00 PM RT (Expected) 1.28(1.36) 1.25 (1.36) 2.03 (2.08) 0.00 (2.44) 2.09 (2.12) 2.09 (2.00) 3.10 (3.06) 3.09(3.04) 3.88 (3.88) 3.89 (3.88) 3.89(3.87) 4.53 (4.53) 4.55 (4.64) 5.06 (5.13) 5.52 (5.56) Area 4114400 3499200 959540 0 2823500 7587400 423110 2754900 498530 239570 3009400 513210 291580 355810 262660 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.29 1.95 0.33 0.00 0.32 0.86 0.15 0.96 0.21 0.10 0.32 0.30 0.17 0.32 0.35 Page 31 of 170 369 p. 94 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review PFCIOppb I1 ion 0* Ut 15 20 PFOA1 413.0/169.0 Da Baseline Missed p Wrnnn Peak 2.09 6*5 4*5 W? ti :c :s Tumi, nun PFOS 1 499.0/80.0 Da Baseline Missed n \A/rnnn Peak 4*5 . 5*4 3^4 10*5 25*5 % 20*5 f , ^ 1UCT 5!.*4 05 I 10 15 2fl 13C-PFOA 417.0/372.0 Da C Baseline Missed n Wrnnn Peak 5 5e5 4*s | 3*5 5 2*5 1*5 ,e 15 20 25 Tan* mn 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak 1*i ~ i s 3.89 25*5 :o5 S 15*5 | 10*5 50*4 I 2 5*5 2 0*5 $ l5*5 J 10*5 5C4 1 15 20 25 Tim* mm 30 15 ro 25 PFNA 1 463.0/419.0 Da Baseline Missed _____ n Wrnnn Peak_____ 4*5 3.08 13C-PFNA j468.0/423.0 Da C Baseline o Missed p Wrnnn Peak 4*V 3*5 & ii "*5 s 1*5 1*5 If i 10 21 30 35 40 25 30 35 Time mm PFDA 1 13C-PFDA 513.0/469.0 Da 515.0/470.0 Da a Baseline q Missed Baseline Missed n Wrnnn Peak r Wrnnn Peak 15*6 X 7 10*5 1I 50*5 U5 5*5 5 1*5 | 3e> SM i c. in j* PFUnAI 563.0/519.0 Da O Baseline Missed n Wrnnn Peak 4.55 ;*5 1V 50 H 40 45 Tune mr 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak 5*4 5*4 & 4*4 t *4 1 -,e, 1*4 14.55 50 30 35 40 4 5 PFDS1 599.0/80.0 Da Baseline c Missed n Wrnnn Peak 2.5*5 a 20*5 b & 15*5 I 1 10* 50*4 I 15 2J3 25 10 13C-PFOS 503.0/80.0 Da D Baseline Missed n Wrnnn Peak 5*1 5*4 9 4*4 22 *4 1 >< I jn j s Tune mm PFDoA 1 in 40 45 5(1 13C-PFDOA 55 45 50 Time irar PFTrA 1 55 60 40 45 50 13C-PFDoA 55 613.0/569.0 Da Baseline Missed n Wrnnn Peak 5[ . 615.0/169.0 Da D Baseline q Missed n Wrnnn Peak 4.55 Ia 4*4 I 663.0/619.0 Da Baseline Missed n Wrnnn Peak_____ 615.0/169.0 Da D Baseline Missed n Wrnnn Peak_____ 5 55 4 PFTeA 1 713.0/669.0 Da Baseline Missed n Wrnnn Peak_____ 4 0 45 50 Tmi9. row; 13C-PFDoA 55 615.0/169.0 Da Baseline Missed r . Wrnnn Peak Before After Page 32 o f 170 370 Ion Ratio Confirmation p. 95 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM PFC 10ppb Before After Page 33 of 170 371 p. 96 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 021710\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb 2/17/2010 7:30:49 PM PFCkinetexLong.dam LCMS2-85F Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 7 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.23(1.36) PFNA1 2.00 (2.06) PFOS 1 2.06 (2.09) PFDA 1 3.05 (3.06) PFUnA 1 3.88 (4.06) PFDS 1 3.88 (3.87) PFDoA 1 4.53 (4.55) PFTrA 1 5.07 (5.06) PFTeA 1 5.52 (5.51) IS Name 13C-PFOA 13C-PFNA 13C-PFOS 13C-PFDA 13C-PFUnA 13C-PFOS 13C-PFDOA 13C-PFDOA 13C-PFDOA IS Area 3368000 2744800 7774400 2957800 209900 7774400 265160 265160 265160 Analyte Area 3142500 5275000 15521000 5644600 4476600 16094000 3656900 1934500 1290400 Exp Cone (ng/mL) 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 Calc. Cone (ng/mL) 18.400 Accuracy (%) 92 17.700 89 18.700 93 18.500 92 20.400 102 18.800 94 22.800 114 21.500 107 19.200 96 Mod? No No No No No No No No No 372 v 2 ^ ' (0 Page 34 of 170 ................................................. / ? Columbia Analytical Services" IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 SJ3C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 RT (Expected) 1.24(1.36) 1.21 (1.36) 2.00 (2.08) 0.00 (2.44) 2.03 (2.12) 2.05 (2.00) 3.06 (3.06) 3.08 (3.04) 3.89 (3.88) 3.88 (3.88) 3.88 (3.87) 4.52 (4.53) 4.53 (4.64) 5.06 (5.13) 5.52 (5.56) p. 97 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Area 7082100 3368000 1812800 0 4981100 7774400 817940 2957800 828080 209900 5244600 895480 265160 608580 427830 M o d ifie d ? No No No No No No No No No No No No No No No C a lc u la te d Ion Ratio (Expected Value) 2.25 1.07 0.34 0.00 0.32 0.50 0.14 0.52 0.18 0.05 0.33 0.24 0.07 0.31 0.33 373 Page 35 o f 170 Createci with Anal^fstReporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review PFC 20ppb ->4S 3 10' i0*4 l PFOA1 413.0/169.0 Da Baseline Missed n Wrnnn Peak ' 0*4 2 . 0 6 *05 * 50*5 3 0*5 :o*s 75*5 2OeS X 1SoS % 1 ,M 5 0-J 55 i lfi 15 2t 13C-PFOA 417.0/372.0 Da c Baseline Missed n Wrnnn Peak 5e5 s ** i 0 1 I 2*5 l5 ?os 3*5 8 i l i 15 tO Tuns min PFNA 1 75 ! '" 5 | 0*5 s 50*4 20 i 15 20 Tim*, mm 13C-PFNA 25 463.0/419.0 Da 468.0/423.0 Da d Baseline Missed n Wrnnn Peak c Baseline Missed n Wrnnn Peak 7*5 US 65 > 45 S c 0*5 I 2S '.5 13 ,0 5 8 1 ift 3^8 is :o r 5 to 15 70 75 Jil 75 30 J5 40 75 38 35 PFOS1 13C-PFOS pfda? 499.0/80.0 Da 503.0/80.0 Da 513.0/469.0 Da o Baseline Missed n VA/rnnn Pppk Baseline Missed n Wrnnn Peak a Baseline Missed n Wrnnn Peak 3ff. /*i v. ** ~ StS 7 4**, $ iff; 3^8 >5 4u PFUnXl 45 3.88 ,? if i 50 i 55 40 45 Tsne mm 13C-PFUnA 1M 20*6 8 i Ii 10ft 505 50 0 I 35 40 Tims, nvn PFDS 1 45 563.0/519.0 Da 565.0/270.0 Da 599.0/80.0 Da Baseline Missed D Baseline o Missed Baseline Missed n Wrnnn Peak n Wrnnn Peak n Wrnnn Peak e5 7*5 a vpi ! 4.53 s. 4*4 * 3** 1 2 :< 4.53 4*5 3*5 & 1* 1*5 15.07 1 3C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak SeS r 4*5 & it j*6 S 2*5 lf5 15 70 25 JO 13C-PFOS 503.0/80.0 Da C Baseline Missed n Wrnnn Peak 5e4 - 4*4 8 sI4- 3*4 :#4 4.53 I 4t 45 50 PFDoaI 613.0/569.0 Da Baseline Missed n Wrnnn Peak OfS 75*5 f Clf5 5.52 S .15*6 5 i Of. 411 5 50 Tims rrwfi 13C-PFDOA 55 615.0/169.0 Da Baseline Missed _____ p Wrnnn Peak_____ 4^3 . 44 8 1 *4 : 45 50 55 60 PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak_____ 40 45 50 I n * nw> 13C-PFDOA 55 615.0/169.0 Da Baseline Missed n Wrnnn Peak_____ 50 55 60 Tin*, mir. PFTeA 1 713.0/669.0 Da Baseline Missed n Wrnnn Peak OfO 40 45 50 55 13C-PFDOA 615.0/169.0 Da Baseline o Missed n Wrnnn Peak_____ Before After Page 36 of 170 374 .................................................. /" C o lu m b ia 3 Aoalvtkal p. 99 Created with Analyst Reporter Printed: 18/02/2010 3 24:00 PM Ion Ratio Confirmation ............ ..................... 3*5 $& i ..h A ML ............ ..................... 15 ?0 75 0 PFOA 2 413.0/369.0 Da Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed g Wrong Peak PFC 20ppb nl Drt |1 toes <0*5 .........- 2 03,............ .' 0*5 ........... j j b k .......... 15 ?0 ?5 10 6*5 MU. 5 4*5 !m PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da D Baseline c Misse Wrong Peak :::11 ,, 6*5 < 1 "i 3 ....................... 3 . 59................. ..~~i t ................ n 40 4`j PFUn*A2 563.0/269.0 Da Baseline Missed Wrong Peak | S I5eb - ........ 3 as-- jj.- .. -...- Lv 1.0 15 40 Tun* mtn PFDS2 45 599.0/99.0 Da Baseline Missed a Wrong Peak Tm# nun PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak 7s.m PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak ' t - r. - 1S5 3 10*5 5. \2 50*J V 5ti 1r 5 .... PFTeA2 713.0/169.0 Da Baseline a Missed Wrong Peak Before After Page 37 of 170 375 Created with Anal^stVleporter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 021710\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 50ppb 2/17/2010 7:38:21 PM PFCkinetexLong.dam LCMS2-85G Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 8 5 Standard 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA1 1.21 (1.36) PFNA 1 2.03 (2.06) PFOS 1 2.02 (2.09) PFDA1 3.04 (3.06) PFUnA 1 3.87 (4.06) PFDS 1 3.87 (3.87) PFDoA 1 4.51 (4.55) PFTrA 1 5.07 (5.06) PFTeA 1 5.52(5.51) IS Name 13C-PFOA 13C-PFNA 13C-PFOS 13C-PFDA 13C-PFUnA 13C-PFOS 13C-PFDOA 13C-PFDOA 13C-PFDOA IS Area 3380100 2533800 7717500 2651100 204360 7717500 267090 267090 267090 Analyte Area 8848200 14442000 41433000 14068000 10651000 37888000 7600500 4220200 3542200 Exp Cone (ng/mL) 50.000 50.000 50.000 50.000 50.000 50.000 50.000 50.000 50.000 Calc. Cone (ng/mL) 51.600 Accuracy (%) 103 52.400 105 50.200 100 51.400 103 49.800 100 50.400 101 47.100 94 46.800 94 50.200 100 Mod? No No No No No No No No No X^7 Page 38 of 170 376 IRC Summary Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNIA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 p. 101 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM RT (Expected) 1.23(1.36) 1.23(1.36) 1.99(2.08) 0.00 (2.44) 2.03 (2.12) 2.05 (2.00) 3.10 (3.06) 3.04 (3.04) 3.89(3.88) 3.90 (3.88) 3.87 (3.87) 4.52 (4.53) 4.52 (4.64) 5.06 (5.13) 5.51 (5.56) Area 20502000 3380100 4847600 0 13702000 7717500 2112300 2651100 1929500 204360 13707000 2322100 267090 1870600 1057700 Modified? No No No No No No No No No No No No No No No Calculated Ion Ratio (Expected Value) 2.32 0.38 0.34 0.00 0.33 0.19 0.15 0.19 0.18 0.02 0.36 0.31 0.04 0.44 0.30 Page 39 o f 170 377 p. 102 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review PFC 50ppb 1.98 PFOA 1 413.0/169.0 Da a Baseline Missed n Wrnnn Peak i PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da o Baseline Missed ___ n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak PFUnA 1 563.0/519.0 Da n Baseline Missed r Wrnnn Peak 10*4] Si)? ] ty 1_ 13C-PFUnA 565.0/270.0 Da d Baseline Missed n Wrnnn Peak___ PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak 3.04 L 13C-PFNA 468.0/423.0 Da Baseline Missed ___ n Wrnnn Peak___ 3.04 PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak PFDS 1 599.0/80.0 Da d Baseline o Missed Wrnnn Peak I0e5 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak JL Time, nun 13C-PFOS 503.0/80.0 Da Baseline Missed ___n Wrnnn Peak PFDoA 1 613.0/569.0 Da o Baseline Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da o Baseline Missed n Wrnnn Peak 5D T*, nun PFTrA 1 55 663.0/619.0 Da d Baseline o Missed n W rnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTeA 1 713.0/669.0 Da g Baseline Missed n W rnnn Peak___ 13C-PFDOA 615.0/169.0 Da Baseline Missed n W rnnn Peak___ Before After 378 Page 40 of 170 p. 103 Created with Analyst Reporter Printed: 18/02/2010 3.24:00 PM Ion Ratio Confirmation ..........1:23.............. i PFOA2 413.0/369.0 Da Baseline Missed a Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFC 50ppb PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da Baseline c Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da c Baseline Missed Wrong Peak PFTrA 2 663.0/169.0 Da d Baseline c Missed Wrong Peak PFTeA2 713.0/169.0 Da d Baseline Missed o Wrong Peak Before After Page 41 o f 170 379 Created with Analyst Reporter a Printed: 18/02/2010 3:24:00 PM :al Project Data File Acquisition Method Ewan's ProjectsVPFCs 021710\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used . Instrument Name 021710long.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb ICV 2/17/2010 8:01:01 PM PFCkinetexLong.dam LCMS2-77J Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 9 5 Quality Control 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.24(1.36) IS Name 13C-PFOA PFNA 1 2.04 (2.06) 13C-PFNA PFOS 1 2.06 (2.09) 13C-PFOS PFDA 1 3.06 (3.06) 13C-PFDA PFUnA 1 3.91 (4.06) 13C-PFUnA PFDS 1 3.89(3.87) 13C-PFOS PFDoA 1 4.53 (4.55) 13C-PFDOA PFTrA 1 5.07 (5.06) 13C-PFDoA PFTeA 1 5.52 (5.51) 13C-PFDOA IS Area 3324800 2583600 7449100 2664400 194040 7449100 299150 299150 299150 Analyte Area 3415400 5823700 16562000 5489500 4610900 16977000 3300600 2191400 1582400 Exp Cone (ng/mL) 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 Calc. Cone (ng/mL) 20.300 Accuracy {%) 101 20.800 104 20.800 104 20.000 100 22.700 114 20.800 104 18.300 91 21.600 108 20.800 104 J Mod? No No No No No No No No No 380 $ <<j Page 46 of 170 IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 SJ3C-PFDA PFUnA 2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 p. 105 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM RT (Exp RT } 1.23(1.36) 1.23(1.36) 2.01 (2.08) 0.00 (2.44) 2.05 (2.12) 2.06 (2.00) 3.06(3.06) 3.10(3.04) 3.89(3.88) 3.90(3.88) 3.89 (3.87) 4.53(4.53) 4.53 (4.64) 5.07(5.13) 5.53(5.56) Area 7771100 3324800 1833900 0 5388300 7449100 861070 2664400 1010200 194040 5658600 946530 299150 602970 585430 Modified? No No No No No No No No No No No No No No No Ratio Confirms ID YES No YES No YES No YES No YES No YES YES No YES No Page 47 of 170 381 p. 106 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review I I 1.23 k PFOA 1 413.0/169.0 Da d Baseline Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak PFC 20ppb ICV PFNA 1 463.0/419.0 Da Baseline Missed p Wrnnn Peak 3.06 13C-PFNA 468.0/423.0 Da a Baseline Missed __ n Wrnnn P eak___ 3.10 PFOS 1 499.0/80.0 Da Baseline Missed n W rn nn Peak 13C-PFOS 503.0/80.0 Da q Baseline a Missed n Wrnnn Pnak Tam. mm PFDA 1 513.0/469.0 Da Baseline Missed Wrnnn Pnak 13C-PFDA 515.0/470.0 Da Baseline Missed Wrnnn Pnak PFUnA 1 563.0/519.0 Da Baseline Missed n Wrnnn Peak___ 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrong Peak PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed i Wrong Peak PFDoA 1 613.0/569.0 Da d Baseline a Missed n Wrnnn Peak Turn} mm 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Paak 13C-PFDOA 615.0/169.0 Da Baseline Missed ____p Wrnnn P e ak ___ PFTeA 1 713.0/669.0 Da g Baseline Missed ___ n Wrnnn Peak___ 13C-PFDOA 615.0/169.0 Da o Baseline Missed n Wrnnn Peak Before After 382 Page 48 o f 170 Created with AnalyiV AS^orter Printed: 18/02/2010 3:24:00 PM (i Ion Ratio Confirmation 1.23 PFOA2 413.0/369.0 Da d Baseline Missed d Wrong Peak z jh ' IS 20 24 ICI PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFUnA2 563.0/269.0 Da Baseline c Missed Wrong Peak ............3B9_........ __ .."" '....................... 5ft )4 <0 J5 PFDS~2 599.0/99.0 Da Baseline Missed Wrong Peak PFC 20ppb ICV G*5 5*5 ; J \ .............. 5 4#S 2i1 :v. ...........j j ^ ......... it15 25 ) i PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak PFDA2 513.0/269.0 Da o Baseline Missed Wrong Peak 5*5 1 * | 5*5 ....... 4,S3......... .... .... '....... ' 0 4 50 Tms itct PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak 'rtS* *5i 5 '1*41 t -L Ste rn PFTrA 2 663.0/169.0 Da Baseline Misse Wrong Peak PFTeA2 713.0/169.0 Da Baseline c Missed Wrong Peak Before After Page 49 of 170 383 p. 108 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 02081 OtACalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 02081 OSamplesLong.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb CCV 2/8/2010 7:23:19 PM PFCkinetexLong.dam LCMS2-85F Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 7 5 Quality Control 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.37 (1.36) IS Name 13C-PFOA PFNA 1 2.31 (2.42) 13C-PFNA PFOS 1 2.35 (2.53) 13C-PFOS PFDA 1 3.34 (3.34) 13C-PFDA PFUnA 1 4.12(4.06) 13C-PFUnA PFDS 1 4.12(4.10) 13C-PFOS PFDoA 1 4.76 (4.63) 13C-PFDOA PFTrA 1 5.29 (5.14) 13C-PFDoA PFTeA 1 5.74 (5.74) 13C-PFDOA IS Area 2998700 2406700 7240000 2357900 175930 7240000 223730 223730 223730 Analyte Area 2732300 4688200 14289000 4270400 2867900 14642000 2346700 1564500 1050700 Exp Cone (ng/mL) 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 Calc. Cone (ng/mL) 18.800 Accuracy (%) 94 17.000 85 18.500 93 17.200 86 17.300 86 18.400 92 19.300 96 19.900 99 17.700 89 i Mod? No No No No No No No No No 384 Page 42 of 242 IRC Summary j Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA 2 S_13C-PFDoA PFTrA 2 PFTeA2 p. 109 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM RT (Exp R T ) 1.36(1.36) 1.37 (1.36) 2.32(2.44) 2.29 (2.44) 2.35 (2.53) 2.33 (2.53) 3.32 (3.34) 3.32(3.34) 4.13(4.06) 4.11 (4.06) 4.12(4.10) 4.76 (4.64) 4.75 (4.64) 5.28 (5.13) 5.74 (5.56) Area 6503700 2998700 1438500 2406700 4648000 7240000 660130 2357900 704840 175930 4892700 676160 223730 548290 386040 M od ifie d ? No No No No No No No No No No No No No No No Ratio C onfirm s ID YES No YES No YES No YES No YES No YES YES No YES YES 385 Page 43 of 242 / T CollilTibSS A m ly iK & l S^rvkes" Quantitative Peak Review _1 _ PFOA 1 413.0/169.0 Da c Baseline o Missed n Wrnnn Peak 13C-PFOA 417.0/372.0 Da Baseline a Missed ------n Wrnnn Peak___ ' 2.33 Created with A na lysP ^|ft)rte r Printed: 14/02/2010 6:41:00 PM PFC 20ppb CCV i 2.29 i PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Pnak 13C-PFNA 468.0/423.0 Da Baseline Missed n Wronn Peak___ PFOS 1 499.0/80.0 Da o Baseline o Missed n Wrnnn Pnak 4.12 PFUnA 1 563.0/519.0 Da L Baseline u Missed r Wrnnn Peak i 13C-PFOS 503.0/80.0 Da Baseline Missed Tln Wrnnn Ppak PFDA 1 513.0/469.0 Da Baseline Missed J0*r :*** f 1 1n Wrnnn Pnak 4.12 13C-PFUnA 565.0/270.0 Da Baseline Missed ___n Wrnnn Peak___ PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n W rnnn Peak Cl IWfl 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak PFDoA 1 613.0/569.0 Da c. Baseline Missed n Wrnnn Ppak 13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak PFTrA 1 663.0/619.0 Da Baseline c Missed n Wrnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed n W rnnn Peak___ PFTeA 1 713.0/669.0 Da c Baseline Missed r W rnnn Peak 13C-PFDOA 615.0/169.0 Da Baseline Missed hi W rnnn Peak Before After 386 Page 44 of 242 Created with AnalysPReporter Printed: 14/02/2010 6:41:00 PM Ion Ratio Confirmation 1.36 5 A u l n : PFOA2 413.0/369.0 Da Baseline Missed Wrong Peak ...........1 6 ............ PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFC 20ppb CCV i t`"*i | -JO'. A. A PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak f .vs us PFDA2 513.0/269.0 Da Baseline Misse o Wrong Peak PFUnA2 563.0/269.0 Da Baseline c Missed Wrong Peak PFDS 2 599.0/99.0 Da Baseline o Missed Wrong Peak 4. ____ _____, PFDoA2 613.0/169.0 Da Baseline o Missed Wrong Peak PFTrA 2 663.0/169.0 Da Baseline a Missed d Wrong Peak PFTeA2 713.0/169.0 Da o Baseline Missed c Wrong Peak Before After 387 Page 45 of 242 I Services- p. 112 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020810\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb CCV 2/9/2010 4:51:51 AM PFCkinetexShort.dam LCMS2-85F Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 7 5 Quality Control 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA1 1.26 (1.27) IS Name 13C-PFBA PFPeA 1 3.59 (3.60) 13C-PFHxA PFBS 1 4.26(4.26) 180-PFHS PFHxA 1 5.31 (5.31) 13C-PFHxA PFHpA 1 6.24 (6.23) 13C-PFHxA PFHxS 1 S 180PFHS S 13CPFDA 6.33(6.32) 6.33(6.31) 7.68 (7.65) 180-PFHS 13C-PFDA 13C-PFDA IS Area 4199600 4858000 2765600 4858000 4858000 2765600 3228800 3228800 Analyte Area 8447400 8849500 16466000 8982200 3540400 15250000 2765600 3228800 Exp Cone (ng/mL) 20.00 20.00 20.00 20.00 20.00 20.00 1.00 1.00 Calc. Cone (ng/mL) 18.90 Accuracy (%) 95 19.00 18.70 95 94 18.30 92 18.90 94 18.50 93 0.97 97 1.00 100 Mod? No No No No No No No No > Page 41 of 245 388 iyrrsbia IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA p. 113 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Exp R T ) 1.26(1.27) 3.59 (3.60) 4.26 (4.26) 5.31 (5.31) 6.24 (6.22) 6.33 (6.32) 1.26(1.26) 5.31 (5.30) Area 8447400 8849500 6771900 875300 9806100 6248700 4199600 4858000 M o d ifie d ? No No No No No No No No Ratio C onfirm s ID YES YES YES YES YES YES No No Page 42 of 245 389 Columbia Analytical p. 114 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Quantitative Peak Review 1v>', 0*5 20-rt OS 1.26 ( in IS 6*S SS 9 4 | 3*6 2 ;s 20 05 1^5 I0 15 20 PFBA T 13C-PFBA 213.0/ 169.0 Da 217.0/ 172.0 Da c Baseline Missed c Baseline Missed 4*6 n Wronn Peak 4.26 l6 IS 40 1 4S n Wronn Peak 6?**56 6.33 6*6 e s*5 i 5 1 J6 SO i*5 SS 00 l OS 70 PFC 20ppb CCV 15*C *1% uw 50*6 3^ i;*s 1or. *I 80*5 605 3 Am 20*5 It 35 40 45 45 50 ITn*.min55 60 PFPeATM 13C-PFHXA 263.0/219.0 Da 315.0/270.0 Da Baseline Missed C Baseline Missed n Wrnnn Peak n Wrnnn Peak ?Ori 8 ' | itw 60*5 4S 5.31 SO 1 SS 12*6 )0*6 1i9| 8.0*5 6046 2(l5 60 4S I SO 55 60 PFBS T 299.0/80.0 Da Baseline Missed n Wronn Peak 6.^4 *(wS |t 40.6 JO*? `s 66 f,', Till* mir, PFHpA 1 363.0/ 169.0 Da /1, Baseline Missed n Wronn Peak 8*5 6.33 7*5 & 5*5 1m ;*5 leS 0*0 SS l 60 6S 70 S 18 0 -PFHS 403.0/ 103.0 Da D Baseline Missed n Wronn Peak 18 0 -PFHS 403.0/ 103.0 Da Baseline Missed n Wrnnn Peak 1?*G 10*6 3 . iSi KC^5 4i5 5.31 :os 46 L 60 SS 60 PFHx a I 313.0/269.0 Da Baseline Missed n Wrnnn Peak 4*6 6.33 8 il :*6 1*6 SS CO 65 Ml 130-P F H x A 315.0/270.0 Da Baseline Missed n Wrnnn Peak 8*5 7*5 5*5 5*5 $ 4*5 I 3*5 1*5 SS I 60 ts 70 13C -P F H x A PFHxSI 18 0 -PFHS 315.0/270.0 Da 399.0/80.0 Da 403.0/ 103.0 Da Baseline q Missed q Baseline Missed Baseline Missed ____________________ n W rnnn Peak______ 7.68 06 n Wrnnn Peak 7.68 S6 n Wrnnn Peak 7.^8 SeS iI * 2*5 *|I 4*5 e 5*5 .* E 4*5 2eS i 7R 75 0 13C -P F D A 85 ... 70 J S 60 Tun min S 13C-PFDA 65 13C -P F D A 515.0/470.0 Da 515.0/470.0 Da 515.0/470.0 Da Baseline Missed Baseline Missed n Wrnnn Peak______ _________ n Wrnnn Peak_____ Baseline Missed n Wrnnn Peak______ Before After Page 43 of 245 390 p. 115 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation T.26 "3.59" PFBA2 213.0/169.0 Da o Baseline Missed Wrong Peak PFPeA2 263.0/219.0 Da Baseline a Missed o Wrong Peak PFC 20ppb CCV .... -...... Af 6.... --....... .....| PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak Ite image is nolavaiable PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxS2 399.0/99.0 Da Baseline Missed a Wrong Peak S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After N/A Baseline Missed Wrong Peak Page 44 of 245 391 OslilflfltlMlS cLbJ Analytical Scn/kcs" p. 116 Created with Analyst Reporter Printed! 15/02/2010 2;34:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 020910\CalShort.wiff PFCkinetexShort.dam Result Table Algorithm Used Instrument Name 02081 OSamplesShortedit.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb CCV 2/9/2010 4:46:43 PM PFCkinetexShort.dam LCMS2-85F Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 7 5 Quality Control 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFBA 1 1.26 (1.27) IS Name 13C-PFBA PFPeA 1 3.59 (3.60) 13C-PFHxA PFBS 1 4.25 (4.26) 180-PFHS PFHxA 1 5.30 (5.31) 13C-PFHxA PFHpA 1 6.22 (6.23) 13C-PFHxA PFHxS 1 S 180PFHS S 13CPFDA 6.31 (6.32) 6.31 (6.31) 7.65 (7.65) 180-PFHS 13C-PFDA 13C-PFDA IS Area 4135000 4792800 2867200 4792800 4792800 2867200 3653800 3653800 Analyte Area 8180100 9018900 16669000 8674800 3405100 15506000 2867200 3653800 Exp Cone (ng/mL) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Calc. Cone (ng/mL) 18.60 Accuracy (%) N/A 19.70 N/A 18.20 17.90 N/A N/A 18.40 N/A 18.10 N/A 0.89 N/A 1.00 N/A Mod? No No No No No No No No xOXX' X i i o -2- ( i > Page 225 of 245 392 IRC Summary Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA p. 117 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM RT (Exp RT) 1.26(1.27) 3.59(3.60) 4.25 (4.26) 5.30(5.31) 6.23 (6.22) 6.31 (6.32) 1.26(1.26) 5.30 (5.30) Area 8180100 9018900 6790600 831860 10206000 6310000 4135000 4792800 Modified? No No No No No No No No Ratio Confirms ID YES YES YES YES YES YES No No Page 226 of 245 393 / X Colum bia **J Analytical Services" Quantitative Peak Review p. 118 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM PFC 20ppb CCV Before After 394 Page 227 of 245 p. 119 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM Ion Ratio Confirmation 3:59 z PFC 20ppb CCV PFBA2 213.0/169.0 Da c Baseline Missed Wrong Peak ... ' 6.23 ---------- j & S i 6!. bS ;Ci PFPeA2 263.0/219.0 Da o Baseline Missed Wrong Peak I j*6 |5 141 16 -....... -6.jj.1- ____ ............... .............. M Ci) tb 7b PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak i10C 80', & C&5 1 l(Mi PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak PFHxs" 2 399.0/99.0 Da Baseline Missed Wrong Peak S_13C-PFDA 515.0/470.0 Da 3aseline Misse Wrong Peak NB. PFBA and PFPeA do not have qualifying ion transitions. Before After PFHxA 2 313.0/119.0 Da o Baseline a Missed Wrong Peak N/A Baseline Missed Wrong Peak 395 Page 228 o f 245 Createci with A n a ly it f^orter Printed: 18/02/2010 3:24:00 PM Project Data File Acquisition Method Ewan's Projects\PFCs 021710\CalLong.wiff PFCkinetexLong.dam Result Table Algorithm Used Instrument Name 021710long.rdb IntelliQuan API 5000 Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment PFC 20ppb CCV 2/17/2010 7:53:28 PM PFCkinetexLong.dam LCMS2-85F Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume 7 5 Quality Control 1.00 0.00 Results Summary Analyte Name Analyte RT (Expected) PFOA 1 1.22 (1.36) IS Name 13C-PFOA PFNA1 2.01 (2.06) 13C-PFNA PFOS 1 2.04 (2.09) 13C-PFOS PFDA1 3.06 (3.06) 13C-PFDA PFUnA 1 3.88 (4.06) 13C-PFUnA PFDS 1 3.89(3.87) 13C-PFOS PFDoA 1 4.53 (4.55) 13C-PFDoA PFTrA 1 5.07 (5.06) 13C-PFDoA PFTeA 1 5.54 (5.51) 13C-PFDOA IS Area 3408100 2728400 7860000 2907400 196180 7860000 245450 245450 245450 Anatyte Area 3176200 5790100 15685000 5567500 4174000 16066000 2985300 1967200 1444400 Exp Cone (ng/mL) 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 20.000 Calc. Cone (ng/mL) 18.400 Accuracy (%) 92 19.500 98 18.700 93 18.600 93 20.300 102 18.500 93 20.100 101 23.600 118 23.100 115 i Mod? No No No No No No No No No Page 42 of 170 396 IRC Summary Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2 Created with A nal^rtIm porter Printed: 18/02/2010 3:24:00 PM RT {Exp R T ) 1.21 (1.36) 1.23(1.36) 2.02 (2.08) 0.00 (2.44) 2.05 (2.12) 2.05 (2.00) 3.07(3.06) 3.08 (3.04) 3.88 (3.88) 3.88 (3.88) 3.89 (3.87) 4.53 (4.53) 4.54 (4.64) 5.07(5.13) 5.54 (5.56) Area 7525700 3408100 1780500 0 4989400 7860000 795190 2907400 815730 196180 5355000 852990 245450 577910 445770 M o d ifie d ? No No No No No No No No No No No No No No No Ratio C onfirm s ID YES No YES No YES No YES No YES No YES YES No YES YES Page 43 of 170 397 Created with Analyst f^> o rter Printed: 18/02/2010 3:24:00 PM Quantitative Peak Review 1.23 05 to PFOA 1 413.0/169.0 Da a Baseline Missed n Wrnnn Peak 1 L 13C-PFOA 417.0/372.0 Da Baseline Missed Wrnnn Peak ______ 2 ^ ^ ^ ^ PFC 20ppb CCV i! 2.01 2.01 uL_ PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak 3.06 i 13C-PFNA 468.0/423.0 Da d Baseline Missed n W rnnn Peak PFOS 1 499.0/80.0 Da Baseline Missed r Wrnnn Peak PFUnA 1 563.0/519.0 Da Baseline Missed n Wrnnn Peak 13C-PFOS 503.0/80.0 Da Baseline Missed PFDA 1 513.0/469.0 Da a Baseline Missed JSOOCI . 000 15000 I0IB0 SilOil 3 .8 8 1 M IS <0 45 13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak M & :oc c 1s& 3 .8 9 SOeS SO 30 A n 40 45 pfdsT 599.0/80.0 Da G Baseline Missed n Wrnnn Peak 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Pnak SS 4e5 & 1i 3er' ?*i i5 L 13C-PFOS 503.0/80.0 Da Baseline Missn Wrnnn Peak PFDoA 1 613.0/569.0 Da Baseline Missed n W rnnn Peak 45 SO 13C-PFDOA 615.0/169.0 Da Baseline Missed n W rnnn Peak PFTrA 1 663.0/619.0 Da Baseline o Missed _____ n W rnnn Peak___ 13C-PFDOA 615.0/169.0 Da a Baseline Missed n W rnnn P e ak___ PFTeA 1 713.0/669.0 Da c Baseline Missed n W rnnn Pnak 13C-PFDOA 615.0/169.0 Da Baseline Missed n W rnnn Pnak___ Before After 398 Page 44 of 170 Ion Ratio Confirmation Created with A n a ly ft f ^ o r t e r Printed: 18/02/2010 3:24:00 PM PFC 20ppb CCV PFOA2 413.0/369.0 Da a Baseline Missed Wrong Peak PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak PFOS 2 499.0/99.0 Da Baseline Missed o Wrong Peak PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak i....- -..3,L ............ ...... PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak Time mm PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak .............. 6-, 7... :..:..... I________ ________ PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak 5;54 oew* i PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak Before After Page 45 o f 170 399 p. 124 Acquisition Date Vial Position Sample Name Acquisition Method 1 2/8/2010 18:00 1 IB PFCkinetexLong.dam 2 2/8/2010 18:22 2 PFC O.IOppb PFCkinetexLong.dam 3 2/8/2010 18:30 3 PFC 0.5ppb PFCkinetexLong.dam 4 2/8/2010 18:37 4 PFC 1.0ppb PFCkinetexLong.dam 5 2/8/2010 18:45 5 PFC 5.0ppb PFCkinetexLong.dam 6 2/8/2010 18:53 6 PFC 10ppb PFCkinetexLong.dam 7 2/8/2010 19:00 7 PFC 20ppb PFCkinetexLong.dam 8 2/8/2010 19:08 8 PFC 50ppb PFCkinetexLong.dam 9 2/8/2010 19:23 7 PFC 20ppb CCV PFCkinetexLong.dam 10 2/8/2010 19:30 9 PFC 20ppbICV PFCkinetexLong.dam 11 2/8/2010 20:54 41 K1000911-001 PFCkinetexLong.dam 12 2/8/2010 21:01 42 K1000911-002 PFCkinetexLong.dam 13 2/8/2010 21:09 43 K1000911-003 PFCkinetexLong.dam 14 2/8/2010 21:16 44 K1000911-004 PFCkinetexLong.dam 15 2/8/2010 21:24 45 K1000911-005 PFCkinetexLong.dam 16 2/8/2010 21:31 46 K1000911-006 PFCkinetexLong.dam 17 2/8/2010 21:39 47 K1000911-007 PFCkinetexLong.dam 18 2/8/2010 21:46 48 K1000911-008 PFCkinetexLong.dam 19 2/8/2010 21.54 49 K1000911-009 PFCkinetexLong.dam 20 2/8/2010 22:02 50 K1000911-010 PFCkinetexLong.dam 21 .2/8/2010 22:09 51 K1000911-011 PFCkinetexLong.dam 22 2/8/2010 22:17 52 K1000911-012 PFCkinetexLong.dam 23 2/8/2010 22:24 53 K1000911-013 PFCkinetexLong.dam 24 2/8/2010 22:32 54 K1000911-014 PFCkinetexLong.dam 25 2/8/2010 22:39 55 K1000911-015 PFCkinetexLong.dam 26 2/8/2010 22:47 56 K1000911-016 PFCkinetexLong.dam 27 2/8/2010 22:55 57 K1000911-017 PFCkinetexLong.dam 28 2/8/2010 23:02 58 K1000911-018 PFCkinetexLong.dam 29 2/8/2010 23:10 59 K1000911-019 PFCkinetexLong.dam 30 2/8/2010 23:17 60 KQ1000532-05 PFCkinetexLong.dam 31 2/8/2010 23:25 61 KQ1000532-06 PFCkinetexLong.dam 32 2/8/2010 23:32 62 KQ1000532-04 PFCkinetexLong.dam 33 2/8/2010 23:40 63 K1000821 -001 PFCkinetexLong.dam 34 2/8/2010 23:48 64 KQ1000453-01 PFCkinetexLong.dam 35 2/8/2010 23:55 65 KQ1000453-02 PFCkinetexLong.dam ^ 2/9/2010 0:03 66 KQ1000453-03 PFCkinetexLong.dam 37 ^ ^ /9 /2 0 1 0 0:10 21 K1000968-001 PFCkinetexLong.dam 38 2/9/2010 0:18 22 K1000968-002 PFCkinetexLong.dam 39 2/9/2010 0:25 23 K1000968-003 PFCkinetexLong.dam 40 2/9/2010 0:33 24 K1000968-004 PFCkinetexLong.dam 41 2/9/2010 0:40 25 K1000968-005 PFCkinetexLong.dam 42 2/9/2010 0:48 26 K1000968-006 PFCkinetexLong.dam 43 2/9/2010 0:55 27 K1000968-007 PFCkinetexLong.dam 44 2/9/2010 1:03 28 K1000968-008 PFCkinetexLong.dam 45 2/9/2010 1:11 29 K1000968-009 PFCkinetexLong.dam 46 2/9/2010 1:18 30 K1000968-010 PFCkinetexLong.dam 47 2/9/2010 1:26 31 K1000968-011 PFCkinetexLong.dam 48 2/9/2010 1:33 32 K1000968-012 PFCkinetexLong.dam 49 2/9/2010 1:41 33 K1000968-013 PFCkinetexLong.dam 50 2/9/2010 1:49 34 K1000968-014 PFCkinetexLong.dam 51 2/9/2010 1:56 35 K1000968-015 PFCkinetexLong.dam 52 2/9/2010 2:04 36 K1000968-016 PFCkinetexLong.dam 53 2/9/2010 2.11 37 K1000968-017 PFCkinetexLong.dam 54 2/9/2010 2:19 38 K1000968-018 PFCkinetexLong.dam 55 2/9/2010 2:26 39 K1000968-019 PFCkinetexLong.dam 56 2/9/2010 2:34 40 K1000968-020 PFCkinetexLong.dam 57 2/9/2010 2:42 67 KQ1000620-03 PFCkinetexLong.dam 58 2/9/2010 2:49 68 KQ1000620-04 PFCkinetexLong.dam File Name 02081 ObVCalLong.wiff 020810b\CalLong.wiff 02081 ObVCalLong.wiff 020810b\CalLong.wiff 02081 ObVCalLong.wiff 020810b\CalLong.wiff 020810b\CalLong.wiff 020810b\CalLong.wiff 02081 ObtCalLong.wiff 020810b\CalLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SampiesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 02081 ObtSamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SampiesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 02081 ObVSamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 02081 Ob\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SampiesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 02081 ObtSamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\SampiesLong.wiff 020810b\SamplesLong.wiff 020810b\SamplesLong.wiff 020810b\Samp!esLong.wiff 02081 ObVSamplesLong.wiff 02081 ObVSamplesLong.wiff Inst IS Area Counts 97.23 2198200 2150700 2119100 2111500 2127100 2039400 1935900 2357900 1968300 2512500 2083500 2400200 2369600 2521100 2224100 2347900 2106600 2328400 2355200 2468000 2278600 2235100 2227400 2614100 2632800 2639200 2376700 2419900 2560600 2412900 2564200 3603100 2633300 2721500 2759400 2675300 2837200 2691200 2170500 2575600 2634200 2338800 2593000 2591600 2271400 2291100 2448400 2379200 2308700 2253600 2563500 2428300 2563900 2398200 2174000 2263400 2374100 - z . / ' k f l 400 p. 125 Acquisition Date Vial Position Sample Name Acquisition Method 1 2/9/2010 3:06 1 IB PFCkinetexShort.dam 2 2/9/2010 3:35 2 PFC O.IOppb PFCkinetexShort.dam 3 2/9/2010 3:44 3 PFC 0.5ppb PFCkinetexShort.dam 4 2/9/2010 3:54 4 PFC I.Oppb PFCkinetexShort. dam 5 2/9/2010 4:04 5 PFC 5.0ppb PFCkinetexShort.dam 6 2/9/2010 4:13 6 PFC10ppb PFC kinetexShort. dam 7 2/9/2010 4:23 7 PFC 20ppb PFCkinetexShort. dam 8 2/9/2010 4:32 9 2/9/2010 4:51 y 8 PFC 50ppb PFCkinetexShort.dam 7 PFC 20ppb CCV PFCkinetexShort.dam 10 2/9/2010 5:01 9 PFC 20ppb ICV PFCkinetexShort.dam 11 2/9/2010 6:46 41 K1000911-001 PFCkinetexShort.dam 12 2/9/2010 6:56 42 K1000911-002 PFCkinetexShort.dam 13 2/9/2010 7:05 43 K1000911-003 PFCkinetexShort.dam 14 2/9/2010 7:15 44 K1000911-004 PFCkinetexShort.dam 15 2/9/2010 7:24 45 K1000911-005 PFCkinetexShort.dam 16 2/9/2010 7:34 46 K1000911-006 PFCkinetexShort.dam 17 2/9/2010 7:43 47 K1000911-007 PFCkinetexShort.dam 18 2/9/2010 7:53 48 K1000911-008 PFCkinetexShort.dam 19 2/9/2010 8:03 49 K1000911-009 PFCkinetexShort.dam 20 2/9/2010 8:12 50 K1000911-010 PFCkinetexShort.dam 21 2/9/2010 8:22 51 K1000911-011 PFCkinetexShort.dam 22 2/9/2010 8:31 52 K1000911-012 PFCkinetexShort.dam 23 2/9/2010 8:41 53 K1000911-013 PFCkinetexShort.dam 24 2/9/2010 8:50 54 K1000911-014 PFCkinetexShort.dam 25 2/9/2010 9:00 55 K1000911-015 PFCkinetexShort.dam 26 2/9/2010 9:09 56 K1000911-016 PFCkinetexShort.dam 27 2/9/2010 9:19 57 K1000911-017 PFCkinetexShort.dam 28 2/9/2010 9:29 58 K1000911-018 PFCkinetexShort.dam 29 2/9/2010 9:38 59 K1000911-019 PFCkinetexShort.dam 30 2/9/2010 9:48 60 KQ1000532-05 PFCkinetexShort.dam 31 2/9/2010 9:57 61 KQ1000532-06 PFCkinetexShort.dam 32 2/9/2010 10:07 62 KQ1000532-04 PFCkinetexShort.dam 33 2/9/2010 10:55 21 K1000968-001 PFCkinetexShort.dam 34 2/9/2010 11:04 22 K1000968-002 PFCkinetexShortdam 35 2/9/2010 11:14 23 K1000968-003 PFCkinetexShort.dam 36 2/9/2010 11:23 24 K1000968-004 PFCkinetexShort.dam 37 2/9/2010 11:33 25 K1000968-005 PFCkinetexShort.dam 38 2/9/2010 11:42 26 K1000968-006 PFCkinetexShort.dam 39 2/9/2010 11:52 27 K1000968-007 PFCkinetexShort.dam 40 2/9/2010 12:01 28 K1000968-008 PFCkinetexShort.dam 41 2/9/2010 12:11 29 K1000968-009 PFCkinetexShort.dam 42 2/9/201012:20 30 K1000968-010 PFCkinetexShort.dam 43 2/9/2010 12:30 31 K1000968-011 PFCkinetexShort.dam 44 2/9/2010 12:40 32 K1000968-012 PFCkinetexShort.dam 45 2/9/2010 12:49 33 K1000968-013 PFCkinetexShort.dam 46 2/9/2010 12:59 34 K1000968-014 PFCkinetexShort.dam 47 2/9/2010 13:08 35 K1000968-015 PFCkinetexShort.dam 48 2/9/2010 13:18 36 K1000968-016 PFCkinetexShort.dam 49 2/9/2010 13:27 37 K1000968-017 PFCkinetexShort.dam 50 2/9/2010 13:37 38 K1000968-018 PFCkinetexShort.dam 51 2/9/2010 13:46 39 K1000968-019 PFCkinetexShort.dam 52 2/9/2010 13:56 40 K1000968-020 PFCkinetexShort.dam 53 2/9/2010 14:06 67 KQ1000620-03 PFCkinetexShort.dam 54 2/9/2010 14715 68 KQ1000620-04 PFCkinetexShort.dam 55 2/9/201O l ^ 7 PFC 20ppb CCV PFCkinetexShort.dam 56 2/9/2010 16:56 23 KQ1000620-01 PFCkinetexShort.dam 57 2/9/2010 17:05 24 KQ1000620-02 PFCkinetexShort.dam 58 2/9/2010 17:15 22 KQ1000620-05 PFCkinetexShort.dam 59 2/9/2010 17:24 21 KQ1000620-06 PFCkinetexShort.dam File Name 020810\CalShort.wiff 020810\CalShort.wiff 02081 OVCalShort.wrff 020810\CalShort. wiff 02081 OVCalShortwiff 020810\CalShort.wiff 020810\CalShort.wiff 020810\CalShort.wiff 02081 OVCalShort.wiff 020810\CalShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort,wiff 020810\SamplesShort. wiff 020810\SamplesShort.wiff 02081 OVSamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort. wiff 020810\SamplesShort.wiff 02081 OtSampfesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 02081 OVSamplesShort.wiff 02081 OVSamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 02081 OtSamplesShort.wiff 02081 OVSamplesShort.wiff 020810\SamplesShort.wiff 02081 OVSamplesShort.wiff 020810\SamplesShort. wiff 020810\SamplesShort.wiff 02081 OVSamplesShort.wiff 020810\SamplesShort.wiff 02081 OVSamplesShort.wiff 020810\SamplesShort. wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort.wiff 020810\SamplesShort. wiff 020810\SamplesShort.wiff 02081 OVSamplesShort.wiff 020810\SamplesShort. wiff 020810\SamplesShort.wiff 020910\CalShort. wiff 02091 OVSamplesshort.wiff 020910\Samplesshort.wiff 020910\Samplesshort. wiff 02091 OVSamplesshort.wiff IS Peak Area (cour 0 2744000 2965500 2843300 3213800 2957400 3316000 3257900 3228800 3123300 4639400 4186200 4335800 4331900 4658500 4217300 4572000 4525200 4211300 4377000 4862200 4209400 3692800 4233000 4149500 4358600 4680100 4170300 4549400 4359600 4200600 4457300 5140400 5160100 5105600 4189100 4413900 4342000 5041100 4419900 4747500 4581000 4121500 4777100 4580700 4274300 4641000 4894400 4569300 4648100 4305000 4136100 4586500 4359900 3653800 4280400 4022000 3961000 4230300 401