Document KGZY4yzVqm23VrNqonRNLNpe0

AR226-2848 E. I. du Font: de Neoours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 66-76 Ma terial Tested Haskell No. 9542-3 Other Codes Orga CARDIAC SENSITIZATIOM to|HfJfJUJk Ten healthy male beagle dogs were exposed to the test mixture via face maski( 1while having their electrocardiogram recorded on Lead I; the two active electrode_s__were _pl Ifc^t sides of the chest and the indifferent electrode over midback. The test animals received a con ne'brine (0.008 nig/kg) intravenously five minutes prior to exposure and a challenge inject'.on of the breathing the test mixture for five minutes. Exposure was continued for five challenge injection to observe recovery. The test material, a liquid, was vaporized by bubbling four liters of air per minute through a containing 60 milliliters of test compound. The scrubber was housed in a warm water bath (38 C). A material remaining in the scrubber was discarded and replaced with a fresh sample. The vapor mixture reaching the dog was monitored by two sampling methods. (1) A one-half mill. taken every minute during exposure with a gas tight syringe from the line leading to the dog mask. the mixture 0.33 to 1.0 liters per minute was drawn from a point near the dog mask through two imping of absolute ethanol. Both the air and impinger samples were analyzed on a gas chromatograph equipped ioni tion detector and a 6' x 1/8" stainless steel, 10% FFAP on chromasorb W column. Only the main was measured analytically. Standards :had been made previously by using a relatively pure (93.5%) sam Results: The mean exposure concentration o^------Bkas measured by air samples was 110.3 ^ 11.1 mg/L As measured by Impinger, it was 104.5 -t_ 15.6mg7L(5729 + 855 ppm). The concentrations of the other relatively low in relation to the main~component, especially the "high boilers." The concentration (not the main component) decreased over the course of the exposure. During the exposure the color o N0^ scrubber changed from clear purple to clear yellow. Results (Continued) - 2 - None of the ten dogs tested had any ssrioua arrhythmias as judged by the criteria of the cardia test (multiple consecutive ventricular beats or ventricular fibrillation). During the first minute o 4UBBB tihe animals struggled--perhaps due to Lhe presence of an irritating constituent. t<o attempt was made to expose dogs to a higher test concentration, since based on the design o animals were for all practical purposes exposed to a saturated vapor. According to the conditions of this study, concentrations of 105-110 rog/L fiMB|B the mai did not produce cardiac sensitization in any of the animals tested. )t0Dace Issued: January 30, 1976 Report No. 66-76 Report by: Approved by; ^^i^ .^ / ^-<^ Linda S. Mullin Physiologist ^7^^6 Nell D. Krivanek Chief, Physiology Se