Document KGEvpremjRzzjQxNrj5Jqomz2
*
.
Form6T47-11-A
TECHNICAL REPORT SUMMARY
Dom Jan . 8, 1979
TO: TECHNICAL COMMUNICATIONS CENTER - 201-2CN
^(Important - If report it printed on both ados o f paper, send two copies to TCC.)
OhrlPon E nvironm ental L ab o rato ry
arelaet F ate of F luorochem ical P ro je c t
Report Title A q u a tic T o x i c i t y S t u d i e s ; FM 3925
To D. L. Bacon
Author!)
A. N. W e lte r
Notebook Reference
Bpt dumber 0535
Prefect Number R9e9po7r0t N0u1m2be6r 0 0
018
Employe Numberta)
09362
too. of Pepoe Including Covanhaot
^SECU RITY
Icompeny Confidential)
f^ ^ M A u th o riza tio n )
^3M CH EM ICA L *R EG ISTR Y
New Chem icsli Reported
O Yae
C*No
KEYW ORDS: (Satoet term from 3M
Thesaurus. Suggest other
applicable terms.)
EE & PC D iv . (Environm ent a l Lab)
CU RREN T OBJECTIVE:
To d e t e r m i n e t h e a c u t e 96 h o u r LG5Q v a l u e f o r b l u e g i l l s u n f i s h ( Lepomie noovookirm ) a n d t h e w48 h o u r LC,. v a l u e f o r Dc^pkrda magna e x p o s e d t o v a r y i n g c o n c e n t r a t i o n s o f FM 3 9 2 5 .
(A quatic) )
R EP O R T A B S T R A C T : (200-250 word) T h it abstract information to distributed by th t Tachnicol Communications Cantor to tort 3M 'an to Company R&D- It to Company confidential matarial.
T h e l i m i t e d w a t e r s o l u b i l i t y o l FM 3925 p r o h i b i t e d a d e fin itiv e determ in atio n of th e aq u atic to x ic ity of t h i s t e s t m a te r ia l. B oth v e r te b r a te and in v e rte b ra te L C -,, v a l u e s e x c e e d t h e w a t e r s o l u b i l i t y o f FM 3 9 2 5 .
50
ic : R, A. P ro k o p D,, R i c k e r B. L. Bohon M. T . E lnabarajw y A. M endel E, A. R e in e r S. K. W elsh
Inform atioon Liaison iI
initial: s V u )
CONCLUSION
T he l i m i t e d w a te r s o l u b i l i t y o f FM- 3B25 p r o h i b i t e d a d e f i n i t i v e
determ ination of th e aq u atic to x ic ity o f th is te s t m a te ria l.
B o th v e r t e b r a t e and i n v e r t e b r a t e LC_ft v a l u e s e x c e e d t h e w a t e r
s o l u b i l i t y o f FM 3 9 2 5 .
ou
INTRODUCTION
The t e s t s u b s ta n c e FM 3925 i s an o f f - w h i t e s o l i d . T e s t s p erfo rm ed on t h i s m a te ria l e v a lu a te d i t s a q u a tic t o x i c i t y and th e d a ta o b ta in e d form th e b a s is o f t h i s r e p o r t.
METHODS
A. A q u a tic T e s tin g
The t e s t p ro to c o ls u ti l i z e d f o r th e s e s tu d ie s w ere m odeled a f t e r t h a t d e s c r ib e d by US/EPA (1 9 7 5 ).
B. A cclim atio n P ro ced u re
The b l u e g i l l s u n f i s h (Lepomie m aazvehirus) w e re o b t a i n e d from a p riv a te h a tc h e ry t. S tock f is h w ere h e ld in a fib e rg la ss holding tank f il le d w ith c a rb o n -filte re d w ell w a te r a t 14-15C . A d a ily p h o to p e rio d o f 16 h o u rs lig h t and 8 hours dark w ith a 30-m inute tr a n s itio n p e rio d was m ain tain ed th ro u g h o u t th e a c c lim a tio n and te s tin g p e rio d . The f is h w ere fe d T etra-M in d a ily , food being w ithheld 48 hours p r io r to and throughout
th e t e s t p e rio d . F ish w ere a c c lim a te d f o r 14 days p rio r to te stin g .
An a c u t e s h o r t - t e r m ( 9 6 - h o u r ) s t a t i c b i o a s s a y w as p e r f o r m e d on FM 3 9 2 5 . C a r b o n - f i l t e r e d w e l l w a t e r o f known c o m p o s i tio n
was used as th e d ilu e n t (A ttachm ent 1 ) . A ll g la s s a q u a r ia (35x20x20 cm) c o n ta in in g 16 l i t e r s o f d i l u e n t o r d ilu e n t p lu s to x ic a n t com prised a stu d y cham ber. Twenty b l u e g i l l s u n f is h , u n ifo rm in s i z e , w ere t e s t e d a t each chem ical c o n c e n tra tio n . T est f is h w ere random ly a llo c a te d to each te s t chamber w ith in 30 m inutes fo llo w in g
to x ic a n t ad d itio n . B lu e g ill su n fish experim ents w ere conducted a t 20-21 C. M o rta lity , te m p e ra tu re , d is s o lv e d
o x y g en l e v e l an d pH o f a l l t e s t s o l u t i o n s w e re m e a s u re d a t 24-hour in te rv a ls o r u n til to ta l m o rta lity had o ccu rred .
G e n e r a l o b s e r v a t i o n s r e l a t i v e t o b e h a v i o r a l c h a n g e s ** w ere s im ila r ly re c o rd e d when a p p r o p r ia te .
These s tu d ie s were perform ed in r e p l i c a t e . O rganism s used in th is study were c o n sid e re d to b e g e n e ra lly h ealth y and fre e of d isea se.
The Daphnia magna u s e d i n t h i s s t u d y w e re o b t a i n e d on A p ril 19, 1978 from th e Mogul C orp. T e s t p ro to c o l u sed
was t h a t d e s c r i b e d by USEPA ( 1 9 7 5 ) .
+ D ale F a ttig F ish H atchery, B rady, N ebraska Com m ercial F ish food of known c o m p o sitio n
RESULTS The b l u e g i l l s u n f i s h {LepcM o mbcero&irua) -oaod i n t h i s s t u d y had an av erag e w eight o f 0 .5 g and le n g th o f 4 .0 cms. The lo a d in g f a c to r fo r th e s e s tu d ie s was ap p ro x im ately 0 .6 3 g / 1 , below t h e maximum recom m ended l o a d i n g f a c t o r o f 0 .8 g / l v ' . T e st te m p e ra tu re s ra n g e d from 18 - 20C, pH v a r i e d fro m 7 .8 - 8 . 2 pH u n i t s a n d o x y g e n s a t u r a t i o n a t 96 h o u rs in th e in d iv id u a l e x p e r im e n ts w ere 34 and 56%. In t h i s r e p l i c a t e d s tu d y , LC-0 v a lu e s w ere n o t c a lc u la te d s in c e th e s e v a lu e s would ex ceed th e w a te r s o l u b i l i t y l i m i t s o f FM 3 9 2 5 . (A tta c h m e n t 2 ) . The Daphnia magna s t u d y was c o n d u c te d a t a t e m p e r a t u r e o f 1 9 C ., pH ra n g e o f R-.2 - 8 .7 and a m in im a l 48 h o u r oxygen s a t u r a t i o n o f 79%. P r o je c te d LC-n v a lu e s e x c e e d e d th e w a te r s o l u b i l i t y o f FM 3 9 2 5 . ( A tta c h m if it 3 ) . The d a ta re p o rte d h e re in are based on s tu d ie s perform ed by M. T . E ln a b a ra w y -a n d L . H. P r i t c h a r d . REFERENCES 1 . US/BPA - M ethods f o r A c u te T o x i c i t y T e s t w ith F is h
M ac ro -in v erteb ra te s and A m phibrians, E co lo g ical R esearch S e rie s 660/3-75-009, 1975.
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ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY DATA SHUT
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ENVIRQNICNTAL ENGINEERING LAIOftATORY AQUAT1C TOXICITY DATA SICET
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ATTACHMENT #2
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ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY DATA SECT
ATTACHMENT #3
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ENVIRONMENTAL ENGINEERING LABORATORY
ATTACHMENT #3 ( ,
AQUATIC TOXICITY DATA SHEET
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WRSDfttV HRY 11 1978 15*34431
i I* W <51
15
--
ENVIROMHEHTRk RESEARCH kAR. - DMkUTH
PRO M T ANALYSIS FOR MORSSAY DATA***
NO OF CURVES ? 1 MO. OF CONS. FOR CURVE 1 ? 4 IMPUT DATA-CONC. rNQ. EXPOSEMNO. DEAD
? 650 2 0 ,0 ? 1000>20>4 T 15502012 ? 2400.20.20
LD6 CONC. ^
FRACTION RESP0R8SM6
2.812813
0
1000
3
0 .2
1550
3.190332
0.6
* 2400
3.380211
1
CHI-SO PRUMRIUTY IS > 0.05
.............
NO OF ITERATIONS
INTERCEPT <a>
SkOPE CB>
6
-24.7904
7.917881
( -TEROGENIETY FRCTOR 8 1
lr,go z
1351.882
95 PCT. kOUER CORF. klHJT
95 PCT.
LIMIT
FITTED
FRRCTIOR .5,
0.005900574 0.1499294 0.6809147 0.9757905
CHI SB FOR LINEARITY
CHI SO FOR PROBRMklTY
1.61074
0.4469226
6 0.1348014
1193.531 - 1533.081
>4 6^
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