Document KG7vY9nVrDVjbaOa6m2O0gkeX
AR 36 -078/
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
IESTSUBSTANCE 00
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95,oras a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS# 2795-39-3).
Remarks: The 3M production lot number was 1. The test sample is FC206. Current information indicates itis a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect toxicity of the fluorochemical componentof the test sample.
METHOD:
Method: Not given.
Type: Acute static and continuous flow
GLP: No
Year completed: 1974
Species:Pimephales promelas
Supplier: Not given.
Analytical monitoring: Temperature, pH, and DO.
Exposure period: 96-hours
Statistical methods: LCs; value calculated using graphic interpolation.
Test fish age: Juveniles Length and weight: Average length = 2 in.
Loading: Not given Average weight = 1.6 g (wet)
Pretreatment: None
Test conditions:
-
Dilution water: Carbon-filtered city water, St. Paul, MN
Dilution water chemistry: Not given.
Lighting: Not given. Stock and test solution preparation: A 50% primary stock solution was prepared for the continuous flow study. The test material was added directly fo the dilution water on a volume/volume basis for the static study. Diluter Flow rate: Not given. Concentrations dosing rate: Continuous for the flow study, once for the static study. Stability of the test chemical solutions: Not noted. Exposure vessels: Not given. Number of replicates: 1
001459
NNuummbbeerroofffciosnhcepnetrrraetpiloincsa:te:fivNeoptlguisvaenb.lank control Water chemistry during the static study:
PH range (0-96 hours):
76..91--77..20 ((c1o2n5t0rolmge/xLpoesxuproes)ure)"
`DTiesmspoelrvaetduroexyrgaenngera(0n-g9e6
hours): 67 (0-96 hours):
72
F
4.7-5.7 mg/L (control exposure)
* 12503m.9 g- 5(.i7nmtegr/mLed(i1a2t5e0comngcleLnterxaptoisounr)ee)x"posure values used
because the highest three concentrations resulted in total mortality by
72 hours.
Water PchHermiasntgrey(d0-u9r6inhgoutrhse):continuous flow study: 6.9~7.0 (control exposure) 7.07.1 (2500 mg/L exposure)*
TDeismspoelrvaetduroexyrgaenngera(0n-g9e6 (h0o-u9r6s)h:our6s7):- 72 F 6.06.3 mglL (control exposure)
4.56.0 mg/L (2500 mg/L exposure)*
b* e2c5a0u0semgth/eLh(iignhteesrtmetdwioatceonccoenncternattriaotniosnr)eesuxlptoesduirnetvotaalluemosrtuasleitdy by 48
hours.
Reswts
00000
mNaoimlinal concentrations: Blank control, 500, 1250, 2500, 3750, and 5000
Element values: 9966--hhoouurr LLCCsso ((sctoantitci)n=uo1u7s5f0lomw)g/=L3000 mg/L
Element values based on nominal concentrations
:
SRuebmsatraknsc:e TReesmtainrgkwsafsielcd.onTdhucetveadluoenstrheepmoirxtteudraepapslydetosctrhiabtemdixitnutrhee aTnedstnot the fluorochemical proportion alone.
c0 onclusi0 ons 00000 `The test sample 96-hour LCso for fathead minnow was determined to be 1750
mg/L under static conditions and 3000 mg/L under continuous flow conditions.
PSauublm,itMtienrn:esot3aM, C5o5m1p33any, Environmental Laboratory, P.O. Box 33331, St.
001460
paraguay
00
aRenldiadboilciutmye:ntKaltiimoins.ch Trhaenksinagmp3l. eTpeusrtiityngwalascknsotrepcroorpderolfy mcehtahraocdtoelroigzyeduasendd tphreopsotrutidoynlaincktsheansaollyuttiicona.l confirmation of the amountoffluorochemical
R0 EFERENCES 000
STth.isPasutlu,dMyNw,asLacbonRdeuqcuteesdtbnyutmhbee3rM20C8o3m,p1a9n7y4,. Environmental Laboratory,
o0 mer 00000
Last changed: 6/27/00
-
001461
.
ce LAB REQUEST 800K -- 425W
[
Waterand Wastewater REQUEST FOR LABORATORY WORK
2 Lab Request N_ o. A0%3
TO: ENVIRONMENTAL ENGINEERING LABORATORY -- BUILDING 53-6N
[--
[ry
aPmreo:toa.leROCOFNob Nro. ecue-- one
DeSHsocredipStoaimoon::ls
i Sa Time: i -- .
a
et
AOnwayean: s 7-- uHoeY SCpooo mpo r--8B FTA?.
TE beeroin statlo]CFow][ [7] CTaoltatlaHaHradnredsnse,sma,f ml5 Ca3C053005
I EE I Tr IIT--
TN=PE" Alkaalinlitiy, mgmg/l tasCC0aCO3s
E 1EII -- E --TTEE-- --11
NSiulfiat:e, mmg/[ lo88N0O r EIr E F BB I
`SCihiso,ridme,imgr/l5ias0C3I
T E T E T T iTE-- 11
OrTiuonridPe,asmg/hlitase FTE
T IE -- T --T TT E--
C; L TTotaal PChohspohamtea, mmg/alasP_Gr
I rrr 11
`THeox.lCChryominum,mmgC/l ais Cr
r E --1r y -- E--
FTerrornocmyaalniadse,Fmeg/l as CN.
r I Ir I--
ManganesemgT fiasWn 111 TTT 1 --7T7
Surfactant, mg/l as LAS
TT
Phenol, mg/l
|I IS
I SS
ES
SO
BOD, mg/l
rT
TOD, mg/1 (Short, Long, Dilute]
rrr
TTT
TVoottalatSluospSepnedneideSdolSidos,l
I r TT T T
Turbidity, mg/l Silica.
111
TTootuall SVoloidus,tmog g
T r T T T
Conductivity, mhos.
TTT
Total Bacteria, No./100 mi
rr
oFcotdal CCoolilftoromn,NNoe./10000omli
E TTTT I TTE--
Focal Streptococcus, No./100 mi
11111
Ammonia Nitrogen, mg/l as N
IA
I ASS A EN
Kiedidah! Nitrogen, mg/l as N
le
They TTTFT
HR torfom Mg Sm Bioa
a
96 ho
g_
\ Comments: K O175kgy
2
20
p00
TT PPMIT ppM [TT -- T1
1B
03 /
te minSr a a ~ Eee Ss aBt ~ Emad Sn. aSl 001o4r 62 Mere
Toi
:
ENVIROMENTAL ENGINEERING LABORATORY
AQUATIC TOXICITY WORK SHEET
_
C0 pe test A CFa Iow) Materialreseed [32lo.f#4 ] 3
rest organise T0thend Minows we. weighe bg HE 2xnehes
Date started _\ _QoT]G date Complete
R-\DTY
Tine seared 2:30 OM
vitution vacer Cc ity wa ll t afte ha{in l BEST COPY AvAILABLE
.
2s
45 hes
7 hes
s6 hes
Cesen3a pi imD.0 sorl*on on pe0.0 seAs pi 30D.0 sd5s D.0
-- HERE --TmPs) \oo_| fe Jee a4 (X%
52001c]o elto| \oo| [57] oo {52 loo Ss
C1250 oo Hei] Noo | 155] \oo. [Sel loo [7247
CB| ve, 22l!iG0 dee!r Ao|: jus' ! 0 2[7)ops
Hj so sO PT 0 13
So.a Mea0l 13m 0 3-10 [17
ES Ltn- HT | bd f| m 1
-
Sd
me Ab|
{
L| l
id
af
SANNATTSO LBLb: Ed ec EE
:
E#i13
!
-
|
:
me meemt e ah
Omen Shalt Wh = stock Solution
]
3582 !
005 ons 025 2375 as
hy Es, "46
So
20. i ko
.
Wa
RRB
RE SE somes
om
-
ENVIROWENTAL ENGINEERING LABORATORY --_-- AQUATIC TOXICITY WORK SHEET
r Type Test Qa |, Shalic
Test organism Tathend Minna Date Started 2-11-714
Material tested [=32 43 of #/
Avg. Weight 16g 885 Taohec Date Completed __\ASey
Time Started
ottocion wacer
a 230 PM.
Cy unel afer
colleen
Ciliel
BEST COPY AVAILABLE
j! i.
Cone. _ Survi+v--a.l
pi
vppos
voor
Survival
pi vpioe
2TWes
survival
pi bpeoe
=
sueval
pn
v0
mm un A~ o He oo | a"150 eIo of A.
Sopp| veo [1156] \oo |ko o
ANS] \oo_ [THA zz]
1250 loo vs) 00 |bul 00 |Jo] \oo [1 sal..
s{3 ee Lae (M195] 50 |bal 0[lao -0
204 dn 52 0
C0
me 7lss] 0
0
+1.
[Tha
|3|
ER E osE ebpny dI L i
--
--
ee B
aandpielitg
Ll
1
i
Tepmasfm, AL 2
og
LbTol
/----005
Rs ads a375 os
Wy Wg
Balt
20
Yo
Go
ES
~
:
THe Shan
001464
ried
) b aE E Hig a
DElir L = wsa | e El EF ieHL EE ane
L Ee i
sl :
roi eEe
3
PE
L Loh
HH
E
Hi Hie
Cen
EEE SLE Fp
Hither
e = fag ast
E
ga HHT
E DEE ETE
e Hf
H
EE
a iT
HH
CTR L Lis Ea EHa ORTA EE
yg AR EE
WE Lee Clg BET re
Teti Bel p | i 75
Eli
Ls
il 69 Fe
Ei
Sy
in em Cam
AT ne nLeIh Tky TeERE S s5fnai|
4
Teide
H
C in H FHTEEEs SHEEN THs HE i a
mee CEtlSHH LiHdEdhfeH
pe
HEE
Ls
\ Sk Pointe hn en ELEeo Eof H haciiHe niiid