Document K8N0zz5eXMxMZz16r8zJ555o

A fi. A S I - O H X * MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE_________________ _ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-602. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 501. The test sample is FC602. Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 61.05% water, 20% ethylene glycol, 2% Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 1% magnesium sulfate, 0.7% xanthan gum, 0.1% starch, 0.1% n-(3-chloroallyl)hexaminium chloride, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:___________________________________________ Method: Microbics Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1988 Species: Photobacterium phosphoreum Analytical m onitoring: pH and osmotic condition. Exposure period: 15-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Microbic's 2% NaCI Reagent Test temperature: Not noted. Stock and te st solution preparation: A primary stock solution was prepared by dissolving 200 mg of test substance in 100 mL of Millipore Milli-QTM water. The pH of the stock solution was 5.5 and adjusted to 6.7. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "apparently completely miscible in Dl water, no color apparent". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. - Number o f replicates: two Number o f concentrations: Four plus a blank control Element basis: Percent light loss 002110 RESULTS Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L Element value: 5-minute EC50 = 210 (190 - 230) mg/L 15-minute EC50 = 220 (200 - 230) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_________________________________ The FC-602 15-minute EC50 for Photobacterium phosphoreum was determined to be 220 mg/L with a 95% confidence interval of 200 to 230 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY_______________________________________________ R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S _____________________________________________ This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number F1151, 1988. OTHER_________________________________________________ Last changed: 6/26/00. 002111 ENVIRONMENTAL LABORATORY MICROBIOS MICR0T0XR SYSTEM RAW DATA LR-- E-LlS L TEST SUBSTANCE : F - b O ^ L ___L.Ia W.T . U t o J c t r __ A V C - P lu < ; H I - a o Q - M l l - i ? U * X o TEST D A T E : . i - A - 8 ANALYST: SAMPLE CUVETTE Bl Cl B2 C2 "TNTTIAC.. FINAL ASSAY CONCENTRATION READING mu L KO) 0 BLANK CONTROL qo DUPLICATE IO O -- FTNT READINGS--------------------------- 5-MIN. 15-MIN. 30-MIN. 115) 1(15) 1(30) 7k 93 73 go ) S bopK ca^e. <BX 109, 43 5~0 Ha B3 J K S O \i H o 3n C3 b u p ir^ 4 c O X 37 39 B4 S e o qH AH aa C4 t> u p h 'c o 4 c <\ 1 *7 - a 6> B5 l o o o H 16 15~ . C5 'D u p lic a te 4 2 1 L> ! S T e s t Su^otA.'ace VvsCous V>reui<\ L l^ u \c \ tocV ; s o \ u t v o n < o rv ^ jio o ryJl M L lo a d e r. h x x \\j ! t i d l - 2frui AflfetvVly O o m p k ^ e ly r o t e ilo le. )n X uoateft Y\o Q c W a ff& F e r t. ^VOC-W. S ln . p hf-- ^ .vS~ uSf<4. t o tH-lv. d itiu ic * AAx 4 O&tyieiic. C l 4 j v-s+ h1W - a d je c t o o n ^ . kxCJL t o o n d i S + o c^ son t^\\V v+ % A Iw o W , s t% X '.O L S0 |o + ,o n I^ n iV la l "^tlutioh s ^ T h lx(/1i ra e n Y io J t ^ / i- j- S ,,* o M e . T ^ n J oSSty cmcenUat i\ ^ [ntH`Ll d t i r i o n s euer*. inHcdoccJ ifctoSk. V . Ah*- Luer<^ +Vi*v t r a v iS ^ r ^ C s c js n ) /rrto-t^e. 002112 MICROBICS MICROTOXR SYSTEM t-rv~_x l i Date. / I -&S> 3LANK RATIOS* Pinal Initial microtoxr data reduction A n a ly s t. FC-teO-z. t . m w r Arc. plus, ------ 5=HTT ------- T5=HI1IT---------- ------ 30=HTtr.---------- 3LANK CUVETTE BLANK RATIO DIFFERENCE RLANK RATIO DIFFERENCE BLANK RATIO DIFFERENCE 1461 Cl 76/O- o.vH S3hco ->.530 0 o 73h~-0.&l\ Se)/Q0-'C^O cA oAi l11 'The blank ratios to r fil and Cl should agree w ith in 0.02. <EAN BLANK RATIOS - B1 BK RATIO + Cl BK RATIO L2 5-MIN. 15-MIN. _____a. g 3 > 7 _____ ____ O 8 k _____ ;_____ 30-MTN. FORMALIZED LIGHT LOSS (i1EFFECT) _ Mean Blank Ratio x Initial Sample Reading Final Sample Reading 1 AMPLE CUVETTE est Concentration !j n s ^ 3 a & o A :5 JOCrO 5-MIN. p 6-SHU 0-70 7 i .030 - /.. 3.07 ........ A-oW f/.U7 15-MIN. p . 5 7 H 0 (c79 hooS^ ). J 3 if. 3 3 . 0 ^ -3 7fa____________ 30-MIN. r? : LIGHT LOSS (XA) - -- C X 100 i+ r 3AMPLE CUVETTE Test Concentration 5-MIN. X A 37 15-MIN. X A 30-MIN. X A 3 lasb3 C3 ^ S 5 "o o ?5 / O D D `L *10 SI SI *>4 >7 >0 SH 7\ 7 8| J l _____ SJ______________ ________ J ____________ EFFECTIVE CONCENTRATIONS (EC's)__ ft)A ../A-___ (Calculated using "P,C" data p a irs .) EC'S 5-MIN. SC20 75" ECen (95X Confld. Limits) \ A a io C n o - z z o s 15-MIN, 30-MIN. so- 3 .3 ,0 ( 2 o o - 3 ^ 0 ) ^ ?>o S*<a- JL -Vor &4ock: *