Document K7LVvJyDyqkXkajK1aEVaNX2
BACK TO MAIN
3M ENVIRONMENTAL LABORATORY
INHIBITORY FFECTS OFL-13492 O N ACTIVATED SLUDGE RESPIRATION
REPORT COMPLETED: July 26, 1995
!
Prepared by: Susan A. Beach Advanced Environmental Biologist 3M Environmental Laboratory
Bldg. 2-3E-09 935 Bush Ave. St. Paul, MN 55144
'!: ':! i ' $ 1 .1
Activated Sludge Respiration Inhibition Page 1 July 26, 1895
SUMMARY The purpose of this study was to evaluate the inhibitory effect of L-13492 on aerobic sludge systems in accordance with the 'Activated Sludge Respiration Inhibition Test', OECD No. 209. The median effective concentration, 3-hour EC50, and the 95% confidence interval were reported as > 1000 () mg/L.
INTRODUCTION
Toxicity tests with aerobic sludge provide a rapid screening method and are reliable predictors of the inhibitory effects of potential contaminants on the operation of activated sludge treatment systems.
This toxicity study measured the respiration rate of activated sludge obtained from a municipal wastewater treatment system and followed decreases in the rate of oxygen consumption over a relatively short period (30-minutes to 3-hours) in the presence of different concentrations of L-13492.
MATERIALS AND METHODS
Test Substance:
L-13492, Lot 2, 45% solids, 5/4/95
Appearance
Clear, colorless liquid
Aqueous Solubility
Apparently completely miscible with water.
Physicochemical Properties: See MSDS. This sample contains 44.9% product, 27.2% isopropyl alcohol and 27.9% water.
Date Received
Not recorded
Storage
In the dark at ambient room temperature (22 2C)
Stock Solution
Reported values . :>
N/A; direct weights were used
The reported values were based on nominal concentrations in the test media at the beginning of Che microbial assay.
Controls:
Functional Control (Reference substance)
`
3,5-dichlorophenol (3,5-DCP). Aldrich Chemical Company, P.O. Box 355, Milwaukee, Wl, 5320. Lot 00410K.Z. Received 9/28/94.
Blank control (untreated)
Two test vessels containing inoculum and nutrient medium, but no test or
reference chemicals, one at the start and the other at the end of the test
series.
-.
BACK TO MAIN
BACK TO MAIN
Activated Sludge Respiration Inhibition Page 3 July 26, 1995
CALCUATIONS
The reported values are based on nominal concentrations in the test media at the beginning of the te s t th e concentrations tested and the corresponding response data derived from the toxicity test are used to calculate, if possible, the medial effective concentration, *EC$o* and the 95% confidence
interval by standard logistic regression methods (i.e., Probit Analysis, Trimmed Spearman-Karber). The EC50 is defined as the concentration estimated to inhibit the rate of respiration by 50% as compared to the controls, after 30-minutes and 3-hours of exposure to the test substance.
RESULTS
There were some difficulties encountered in obtaining test results. The initial screening (limit) test indicated toxicity and the need for a study with lower concentrations. The second study shewed low toxicity. The third st'j_/ was invalid due to unacceptable differences in control respiration rates. The fourth study also ideated low toxicity. The final study was initiated to confirm the second and fourth study results. It is possible that in the first study, an error in test solution preparation resulted in loading the test system with more test substance than planned. This could have induced a greater inhibition in respiration rate.
The definitive percent reduction in respiration rate and the median effective concentration, 'E C s o ', obtained after 30-minutes and 3-hours exposure to the test substance L-13492 and
reference substance 3,5-Dichlorophenol are summarized in Table 1. The median effective concentration, 3-hour ` ECso' of L-13492 to activated sludge was
determined and was reported as > 1000 mg/L. A 95% Confidence Interval could not be calculated.
ACCEPTABILITY CRITERIA
This toxicity test meets quality criteria provided in test guidelines:
1) The two blank control respiration rates were within 15 perceni of each other.
2) The 3-hour percent inhibition induced by the reference substance, ` 3,5-DCP` , was within the range of values historically obtained using this mixed liquor source in this laboratory.
3 ) The reported vaiues were based on nominal concentrations in the test media at the
beginning of the microbial assay.
.
This test was conducted by G.W. Jorgensen as requested by Steven J. Martin of 3M SCD Lab, S t Paul,
mn.
' "
j
GOOD LABORATORYPRACTICES
:;
This study was not conducted under strict Good Laboratory Practices, but was conducted under the standard operating procedures for this laboratory. The raw data was reviewed by S.A. Beach prior to reporting. Copies of all raw data are included with this report.
This study was conducted under 3M Environmental Lab Request number N2169.
BACK TO MAIN
Activated Sludge Respiration Inhibition
Page 4 July 26, 1995
REFERENCES
1. OECD (1984). Activated Sludge Respiration Inhibition Test, Test No. 209. In: Guidelines (or Testing of Chemicals. Organization for Economic Cooperation and Development, Paris, France.
2 OECD 1981. Principles of Good Laboratory Practice, Annex 2, C(81)30 (Final): 7-28. OECD Guidelines for Testing of Chemicals. Organization for Economic Cooperation and Development, Paris, France.
Test Cone.. ma/L 1000 Duplicate EC50
Test Cone., mart10 EC50
TABLE 1
L -1 3 4 A 2 PFRCENT INHIBITION INI RESPIRATION RATE
30-min. Exposure none none
> 1000
3-h<?urs Exposure 3 8
> 1
a s-DICHLOROPHENOL REFERENCE SUBSTANCE
PERCENT INHIBITION IN RESPIRATION RATE
30-min. Exposure '
3-hPUrs EXP0&UL9
25 Not calculable
59 Not calculable
BACK TO MAIN
s t k RO kJ
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
LR JhL Data C j l l M K Analysti'GM' iOO- JL
P a g e _ L . of
Typo Test C * F C Z lc l. v i f l lp J S l u c l y - R g s p i rc v i ^c a J
0<?
ACTIVATED SLUDGE MIXED LIQUQB
,
Date Obtained 6 ^ / z y ? T
Date Used (p f^ lJ jS ~
Mixed Liquor Suspended Solids fMLSSI -?> Y g/L Adjusted to --------------- g/L
MLSS In final test solution t - 5
g/L
Initial pH 6 . 6 (1st test soln. prepared)
Final pH 7 .
(last s^ln. ^ r^ a /e d )
Comments 4AJi..\.r.....M.....,....,..A....L.. u t r i r s e f M fr ~ I hr
o ff
S o lid s . A -ftjeCrf d i t o r f . ^ ` xe d
"'-d L .< y * c f ` u<1
I5SLSU B SIA M
L- ~ ISample; Description
2-
Initial pH of highest cone, tested f l f ) C w /L )
7. /
Adjusted to pH
r C*JC
Comments
REFERENCE SUBSTANCE
Sample Description
*5~- D ir L in< -n^ g jnl___f\t; d<~e*JC.g
C)Initial pH o f stock solution ( /
mg/L) - ~73>_
Comments!]
Adjusted to pH
EXPOSURE CONDITIONS ' 1 .KTest Duration . ^ P S -
Test Temperature Range: IX.C. I & 21.0 C
Comments
r II | <7 X ( f ^ |V\< I y
BACK TO MAIN
27
ENVIRONMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION
Test Substance: Sample Description: C le a r~ (-- Analyst: Date: C ^ 2 S (3 S 1
<j
Test Cone. mg added -m g /1. __ oer 500 mL
B m L -4fe h
JlQlQCl.
JL Q .Q -
mL M t1) water per 500 mL 28V
Z z4
mL syn. sewage
. JL.
ft
mL microbi al 20
Total Volume ____mL___ _
_o 30
S *o
_5ain,ft>^_____ W__gyoU. USI ^S & 2. S VA S y'__ _t__ ^___(__H_q_|V_\_1_j__j-Q....M...C...e_,. fO P f y f o ^ .. ^
T T cm'.
.
iV tc, M<. - f j v t tfe-Vbg I T i e T ^ V u e ^ l k j 2 f & r X --
* iM.llifl H rO im i+ p r i o r +o iiQQ Taa-wplg. (As-^yctiOgO-.
.*S k>W!.i .o i-H i M.II.- lU.O , Q . S lvnL q /x rU
S.M O i. | 2 .S~w L
od <|g ^ V.<\ pura.,
-H. s-<-, <oao. . cmI'J 2.1 5/>vL r. f At.ll'.Gi l-)?0 mas added o,flgv- Sa.r^pl<?.
4i I 7 THZ~>0 C vr-(-fnt4 a l
8 Mw LI MM ilI >fQ\ UH .z/ ~O, a d d a c i .l.-.oL'faJ.l `. *S *.>,, pI L.s tQg<~c
.^L^frW .ct k y
T *l u M twi * g d ~Uiflitflkl.
(1) Millipore Milli-Q^ high purity water
BACK TO MAIN
P3 3 c rf7
ENVIRONMENTAL LABORATORY
LR A J 2 / 6 9
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
~' ' Test Substance: 1 1^
2 ____________________
Source of Activated Sludge Mixed Liquor: ftlM ra I
; Date Received: ( p f e t f M S
y'-:- Analvst: G i ^ D
Q* Date Used: G
S .
| 30-MINUTE EXPOSURE
Test Substance 1 Concentration ( ^ / L ) 0 .5
1.0
DISSOLVED OXYGEN CONCENTRATIONS, mg/L
TIME- MINUTES
Temp
1.5 2.0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 C
E
fi BKCONTROL 1
i v bn-- * .. ,
> |j
F o o T 1)
B u i5 . ....
to ZD . 7G..GIp7,5 7.0 CM7 s 7 ,2 G7 , 3 7 , 0
7 -3
GS
S S L S S 5 ,7
,(p Q ^ 7 , 7 S i ( L S S
< c.
5 . 21 M
SS
G<Z .3
JLS
/? & 2 1 .0 20. 5"
rfe' ' V
J p j BKCONTROL It -
&3 Z l
f-'T -
.f:j; ^
V"
iI 3-HOUR EXPOSURE
'
r,o 7,7 7 . S ' 7 . 7 7 0 6 . *
'>
CM 4 . 1
' `
DISSOLVED OXYGEN CONCENTRATIONS, mg/L V
;:j | Test Substance ; ; Concentration (tW L )
2.0 2.5 C1.0 1.5
TIME MIMUTES
Temp
3.0 3 .5 4 .0 4.5 5.0 5 .5
: BKCONTROL! .
;!' loM *f niH>
to 7I,n3
7.
1n,.2i-f
m
7,0
Gu,$
7 ,2 -
7G..G1
7.0 4.3
6.7 7
77 ((7,7
M
f s C.. l~GSi&l -SIS
577
SSL
5."?
/?. 22.00M/
'ii ! -i-. '
||! ' |;i BKCONTROLS
$o r? 7.C 7V 7/
ItllpH of activated sludge mixed liquor: ||[ 1pH of highest concentration tested:
fjli < /%0Te2/.0<:.Test temperature:
Itlitlal litial
-j/-.*...5i'b ,
;;? ':i
FF;InnCaa7,ll,1:;jT'c?'7c,^~m7. WiU|if5?f1*:G.1'*J'!.^*;:;'S7<i'-.?'f-->*fl*'/.s'-s
BACK TO MAIN
'W'&%
.
^ kK :t:'W^'Srl
WPf-W1*?*
i !:* issSa-^.jQ-;
.:a*:-:
I n N?169 Date: 6 /2 9 /9 5
fest Subiti Analyst: j
co co 'Xi
o co m fo co m.
m g/L3 0.5 i l o w r $ * ^ 2 .0 * *?2.5:: 3.0 3.5 4.0 4.5 5.0 5.5 Tomp.*C R (1) Ra (2) % Inh. (3) M w vSMSrWSfcrtto.-.'. *
BK1 ~~ 8.0 8 .0 7 .9 #
7.5 7.3 7.0 6.9 6.6 6.4 6.2 19.0 0.4097 0.4392
1000.0 7.5
" '6 .8 4 6.6 6.2 5.9 5.7 5.2 4.9 4.6 4.3 21.0 0.6448 0.6015
31
oup. 7.3 7.0 - 6.6 6.3 5.9 5.6 5.3 5.0 4.6 4.2 3,9 20.5 0.6812 0.6579
:^V^.
-m M t. >*?>*!,*
-44
V- Stfvs'fr,
- --- '
<V,...v.
BK2 8.3 8.1 8.0 7.7 7.5 7.3 7.0 6.8 6.6 6.4 6.2
f e % ditf in Controls ( 4 ^ ^ 8.2
EC50 (95% CI):
18.8
0.4388 0.4770 RaCm (5): 0.4581
m g/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0
'SKitsSfe
-8K1
7.9* *7 .7 *
7.2 7.0 6.7 6.5
1000.0 *7 .3 17 .2 * * 7 . 0 * 6 .8 6.6 6.3 6.1 5.9
*0UP. * 7 :7 * 7 i6 # 7 .4 7 .2 7.0 6.7 6.6 6.4
* p . 1iiJHMW.I'Ji.' 'MMit'ifi'fri'f m m -
.' ' v i .
SiS&'ty*
.-
lWiSI
MNP
* 8 .&&>
ite s r m m
SBK2 6 A *
m m * 7 . 6 -5*7,4 7.1 6.8 6.7
EC 50,(95% CI): ;
4.5
6.2 5.6 6.1
v>v -
`j.-,%"
6.4
5.0
5.5
*CT e m p .
6.0 5.7 18.6 5.4 5.2 20.4 5.9 5.7 20.1
- W - ' 1W6.1 5.8 18.8
g ? p ig ||
RWflMtuM
B p S ffiftS "
,rj^jsHBPpi
**> I* o m p ^ ^ ^ ^ U U rite m J lO O (Tira corttok muM b wtrtn 16% ot ad o tw
'u*t* dtuati ^ e o t t ^ f u k u n B l ^ ..'.- -, ..
1- m g ^
# 1.072 R*Cn> n w i xtuaM a comot Bk> Uh* canini. 8 MO*fc Tot Mmptotomp * Tobt-20*C
R (1) Ra (2)_ % Inh. (3)
0.4982 0.4545 0.4242
0.5491 0.4421 0.4213
3 8
0.4861 0.5284 RaCm (5): 0.5387
b a c k to main
P^5o-V / U Jsj2j^3
ENVIRNOMENTAL LABORATORY
w lM l m o 3
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION
Test Substance: 3
/)ic {e ra o J )e /Q < 3 /
Sample Description: / U r i r ^
1 Co.
e - < x ( o c l ^Q '~ ^ C C 1^ )C k /Z .
Analyst: G ^ J Z ______ .
Date: __ G ^ 9 f $ 5 ~ . ___
Stock Solution Preparation:.
O
.^
a
lL
j
liriL -- 0 .5 otg
J tiiijk e it 8a' 0 - 5 ^* <n$C'13_,5S.&<CfPf -ThkLt^aQ_iidk_lt_A._____-1__&_:_L c f I M~ fv3.CH n a d cil lu it . f . u P / n ^ 3QnL
nivUttfIta
niirr u'u t i l x yffltliliQ .H l O, CKiLt-Sfa ` '` 'A . u t - l I I I 1 n * . H
ai U
l .L
- f , -u.iot*i\
LLI I ftl
WAci
p\ ^icSt,-tr
lp r'TeUtaix.pj Va-d_cioIfK) g^A.
o f __I At Ht.SC<fl i i LilkeA ip .
-TL j M r k /-.r' ) L. ixM-tK1 t ^ . l l l Q. Ht
.T tu { l 1.p H *. 713 .
Test Cone.
mL added,
~ ftig/l-- . per.50Q,mL
2/<uiLL
/ O Jl
mL MOO) water per SOOmL . 2 *v
X tL
mL syn. sewage feed per 500 mL
____ A _______ AO-
mL microbial
200 200
Total Volume
__ ml____
5~<?Q
TOO
(1) Millipore Milli-Q^* high purity water
'
BACK TO MAIN
ENVIRONMENTAL LABORATORY
LR A M f f l > 4 2 2 0 %
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: 3, S'- D , 'cLforopLtsJo / // //'c'^e ..SvAshisvce
,
Source of Activated, Sludge Mixed Liquor: /Me/ro lOah/e /(In h - I/ea
'PjLd S // / * / (t$0
Date Received:
Analyst:
0? (JCO_______
Date Used:
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mg/L
Test Substance Concentration
TIME- MINLITES
Temp
0 .5 1.0 1.5 2.0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 C
BKCONTROL1
_______ Ip
S.O z.o i 7 .C 7. S 7 .3 7 . 0
G>,(p PM P 2 /J .
5 . 1 t,r) i 7 . 7 7 ,T 7W 7,7- 7 ,0
c.c ~7TW
. nr
-'m
/BKCONTROL : 5 .3 S.i z o 7 7 7 . S 7 . 3 1 .0 C M u C 2. /S.S
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mg/L
Test Substance
Concentration 0i^j) 0.5
wBK CONTROL 1'rti# - io z\-
: %!:* %:;(?$>.jg .'..jf
' ,'f5|i;4;' ;fsV
j .'
!+
. >: -r
"< '
Nf
1.0 1.5 2.0
lv
5,\ w
t .| 7 ,1 li' -
* `
7
id
-r - i:
TIME MINUTES 2.5 3.0 3.5 4.0 4.5 5.0 5 .5
7 . 2 1.0 S I C,sr P.7 C O 5 7 7 7,7 7 ,f 7 ,3 7 . 7 ? 7 ,0
Mr>/
BK CONTROL#
1 L M 7. t 7 & M 7,1
JL2 CM C.l 5.8
;pH of activated sludge mixed liquor: Initial pH of highest concentration tested: . . .I.nitial Test tem perature: IS , & 4 * Z6.z> C.
*5 .0
7 .7
Final 7 7 Final ' ; . x < ' /; ` . .J
Temp C
JO. 3
1 - 'j
:?
! ,1' v i | l!: " ? j; 1
ji
-I!
illS : 'w o r n
HEm
;v fe 2 p fP ' I
H iI f e ' J ||r
BACK TO MAIN
JDGEf
vhi*&&
u -m x , . f , . / .. ` .'- ' ...,,* .lii.
|||||
|# l
'4-;>'.'TV',;.`i
'- .- '
r * : >LR; N2160 & N2208
'r ^ jr
6 /2 0 /9 5
mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5,5 :^ w - ^ 'riJlfct.-St*.
BK1 - 8.0 8.0 7.9 7.6 7.5 7.3 7.0 6.9 6.6 6.4 6.2
rji.yJfc.
^'rvr-
10 8.3 8.1 8.0 7.9 7.7 7.5 7.4 7.2 7.0 6.8 6.6 **&- - A'i: i*V'
i%v.. ..
#8j ; -VVSSK& -. ,
BK2 8.3 8.1 8.0 7.7 7.5 7.3 7.0 6.8 6.6 6.4 6.2 % diff in controls (4): . 8.2
J ' . s g g s i l r -.. 3-Hour Exposure -
Dissolved Oxva en Concentrations. mq/L
m q/L 0.5 - 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
? i&teSs.
S5wl^t i\-~*.-
BK1 '8 . 0 7.9 7.7 7.6 7.2 7.0 6.7 6.5 6.2 6.0 5.7
mem?*-
PW&fc
# '10^ S 8 ; l? # 8 .1 *-7 .9 - *7.8 7.7 7.6 7.4 7.3 7.3 7.2 7.0
S S <M *
.yy:-.
sPSUr i* * S S K #
SS
p s u m isafsg!'
H P
rM-;v
r-****. ft-- '
*BK2 I f l S f flta to f * 7 . 8 ?: 57 .6 7.4 7.1 6.8 6.7 6.4 6.1 5.8
1% 'd iff
1''^`i A\
'rt'i&
5>:
Tsrnp, *C 19.0 19.8
18.8
T*mp. *C 18.6 20.3
18.8
14 n rc*a a**ct. |lRiDl-fii8*21/R*Cm)IOO (Two contro mu to* MNn 16% at Mdi othw )
"IjftJjwawte*
- 1.072 R*Cm* im m 4ul#d R, contsot* Blu U*nk control, 8- Mmpte Tom Mmpto tamp m To0*-2<rc
o> ti*y; na (2) % tnh. (3)
0.4097 0.4392
0.3394 0.3441
25
0.4388 0.4770 RaCm (5): 0.4581
R (D Ra<2) % Inh. (3) 0.4982 0.5491
0.2267 0.2220
59
0.4861 0.5284 RaCm <5): 0.5387
BACK TO MAIN
SSSSiTofCustodvBecord^. ,, *** .
u iim o iu ai u * u vta w r ^
"-:
UobbO
3U Piofta T*
3U FoMy C onuct L V> . V # / . P ? -fe < s# &
3MAoQuMtr
$ A 83 U P iO )ta U M <,A6
/Lo f f r
3M Telephone N utter
3U Project N utter
-Z .0 - O Z ? Z -
JU Deportment Um >\aJ
i0 Rc4m0M'Te^puW Nu^i*
MW
'
^
S
^
K ' w S p t iiW W
t^ d A jQ O G ^ & \-U . i\^ A H i
SSSfSeVhiV'^*A*-^V- 1t -JIL/.V* H -,
a-
iy ? l f f^ m r ; Matrix
j
JWg gFg f f( lK
; ; : '
'&(i&&"\^z'v::T/-
NumPbreonrfWCeVaoIctniVhtMained
-<iX aXn
.....
sr \
1 1 Samples Split See Attachment
Comments
I1
f! 1
I s S l I l l l l I S l ^ i l S tSWSWffE 'gfc *.sy. I # ifi
^ 5 ^ ' :ig f
L,,
P f6?
73fgapPf`. 3 3 * -
i#93l ^.c-v 'v-v: !"-'" :^jO' ^wsfis.:'-.-i-:-
-- ' -- - 1 ' '-' .,-> *v
4 ".
iiV.
ftT ? III?,/ _->
$~4I
s
|j
H
K
W
w
i'l^
fe
> / Relinquished
.
EJyiAffiJ^tfsn
Time
Date
Shipped Via
J Received By/Affiliation
Time
Date
C U m uhJsM
I S f e 8m Beverse8idForInstruction*
04/01/2001
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
BACK TO MAIN
LAB REQUEST NO. N2169 REQUESTOR NAME: SAB/S.J. MARTIN DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 05/17/1995 DESC: L-13492 ECOTOX TESTING
CONTRACT LAB(S): EXP COMP DATE: 07/03/1995 DATE COMPLETED: 01/30/1996 PROJECT LEAD: PHONE NO: 3M FAX NO: 651-778-6176
Results from Microtox Test:
Test Cone. mg/L 125 250 500 1000 EC50 95% C.I. sab 5/19/95
PERCENT LIGHT LOSS
5-min exp.
15-min exp.
13 29
19 43
37 65
75 92
630 300
(590 - 665)
(270 - 330)
30-min exp. 34 51 75 97
260 (220 - 300)
Activated Sludge Respiration Inhibition Test Results: There were some difficulties encountered in obtaining test results. The initial screening (limit) test indicated toxicity and the need for a study with lower concentrations. The second study showed low toxicity. The third study was invalid due to unacceptable differences in control respiration rates. The fourth study also indicated low toxicity. The final study was initiated to confirm the second and fourth study results. It is possible that in the first study an error in test solution preparation resulted in loading the test system with more test substance than planned. This could have induced a greater inhibition in respiration rate. SAB 7/31/95
Test Cone. mg/L 1000 Duplicate
Percent Inhibition in Respiration Rate
3 0 -min Exposure
3-Hour Exposure
0 18
0 22
EC50
>1000
>1000
SAB 7/31/95 NOTE: 3-HR results updated 2/19/96. An error was discovered in calculations of percent inhibition, sab 2/19/96 SEE LR N2332 FOR FISH, DAPHNIA, AND ALGAE TOXICITY TEST RESULTS. SAB 7/31/95
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
1 05/04/95 L-13492
LIQUID
MICROTOX
MICROTOX - EC50
260 MG/L
(220 - 300)
04/01/2001
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
2
LAB REQUEST NO. N2169
SAMPLE DATE
CODE
CONTINUED DESCRIPTION
RESULT
1 05/04/95 L -13492
LIQUID
SLUDG-RESP SLUDGE RESPIRATION - EC50
>1000 MG/L
BACK TO MAIN
MDL or (95% C . I .)
