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FC-17-R Submitted to: BIOSEARCH, INC. p.c.box 8598 philadelphiap,ennsylvania19101 t e Ie p h o n e (2 15 ) 8 4 8 - 4 4 9 9 Project Number 77-1127A 3M Company 3M Center St. Paul, Minnesota 55101 Material: Sample Received: Date of Report: Test: Object of Test: Procedure: 3M Company - T-200ICoC 11/21/77 Study Initiated: 11/23/77 Study Completed: 12/29/77 1/6/78 Acute Oral Toxicity - Rats To study the acute oral toxicity in rats of the subjectmaterial. . Four groups of 5 male & 5 female albino rats of the Sherman-Wistar Strain weighing between 200 and 300 gm were employed in this study. The rats were deprived of food but not water for 24 hours prior to dosing. Each aiiimalwas weighed and dosed by direct administrationof the experimental material into the stomach by means of a syringe and dosing needle. The sample was dosed as supplied. The following dosage levels were administered: 0.50 mi/kg. 2.50 mi/kg. 3.75 ml/kg. 5.00 ml/kg. Results: Conclusion: Following administration the animals were allowed. food and water ad libitum for the 14 day observation period during which time the rats were observed for signs of toxicityand mortalities. See Table 1. The subject material when studied in male and female albino rats has an acute oral LD50 between 0.5 ml/kg. and 2.5 ml/kg. Karl L. Gabriel, V.M.D., Ph.D.Director TABLE I Acute Oral Toxicity Material: 3M Company T-200ICoC, as supplied. Dosage Level mi/kg 0..50 0 50 2.50 2.50 3.75 3.75 5.00 5.00 Number of Animals Dosed 5 males 5 females 5 males 5 females 5 males 5 females 5 males 5 females Mortalities Days 1 2 3 4 5 6 7 8 9 - 10 11 12 13 14 0000 00 000 0 0 0 0 0 oooooo00 0 0 0 0 0 0 31 01 2 3 - - - - - - -- - - - - 3 1 0 0 0 1 --- - - - - 4 1 ------- - - - - 5 -------- - - - - 4 1 ------- - - - - - Total Dead 14 Days 0 0 5 5 5 5 5 5 Total Survived 14 Days5 5 0 0 0 0 0 0 The LD50 is between 0.5 ml/kg. and 2.5 ml/kg. At 0.50 mi/kg. the animalswere slightlydepressedfor 4-6 hours after dosing. At the three higher dosage levels the animalswere comatose within 30 minutes to 4 hours and died as noted. Gross pathologic examination revealed nothing remarkable.