Document K6o2k42d7J8xLbx119YmMyd1K

A ftu 6 -c > 3 7 3 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G. DAPHNIDS) TEST SUBSTANCE Identity: N-methylperfluorooctane sulfonamidoethanol; may also be referred to as N-MeFOSE Alcohol, FC-790, or FM-3925. (1Octanesulfonamide, N-methyl-1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-(2-hydroxyethyl)-, CAS # 24448-09-7) Remarks: Material is an off-white, waxy solid. Sample purity and lot number were not recorded. METHOD Summary of 2 Studies Method: U.S. EPA 660/3-75-009, April, 1975. Type: Static acute GLP: No Year completed: 1978 Species: Daphnia magna Analytical monitoring: Nominal concentrations only. Temperature, pH and dissolved oxygen recorded Statistical methods: Probit analysis. Concentrations tested exceed water solubility of the test substance. Test daphnid source: Obtained from the Mogul Corporation. Test daphnid age at study initiation: Neonates Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry: Hardness*: 236 mg/L as C a C 0 3 DO: 8.9 mg/L COD*: <1 mg/L Alkalinity*: 221 mg/L as C aC 03 pH: 8.7 Conductivity*: 420 pmhos/cm Total Solids*: 286 mg/L * Values taken as averages from general laboratory water quality testing in 1978. Stock and test solution preparation: Not noted in report. 006598 Exposure vessels: Test vessels were described as jars. Each test vessel contained 100 mL of test solution. Number of replicates: one Number of daphnids per replicate: 20 Water chemistry during the study: Dissolved oxygen (recorded at 48 hours or at 24 hours when all test organisms were dead): 7.8 mg/L (control exposure Test 1) 7.6 mg/L (control exposure Test 2) 7.5 mg/L (1000 mg/L exposure Test 1) 8.5 mg/L (8700 mg/L exposure Test 2) pH (recorded at 48 hours or at 24 hours when all test organisms were dead): 8.6 (control exposure Test 1) 8.7 (control exposure Test 2) 8.2 (1000 mg/L exposure Test 1) 8.6 (8700 mg/L exposure Test 2) Test temperature range (0 - 48hours: 19 - 20C Element basis: mortality and immobilization Remarks: A first study was conducted at concentrations too low to induce sufficient mortality to allow the calculation of an LC50. The study was repeated at higher test substance concentrations. The test substance was noted to precipitate out gradually over the course of the test. It was generally noted that more precipitate formed in the higher concentration test solutions. RESULTS Nominal concentrations: Test 1: Test 2: One blank and one solvent control, 100,180, 320, 560, and 1000 mg/L One blank and one solvent control, 650,1 000 ,155 0, 2400, 3700, 5600, 8700 mg/L. Element value: Test 1 48-hour EC5o (95% Cl): Not calculated. Test 2 48-hour EC50 (95% Cl): 1352 (1194-1533) mg/L Statistical Evaluation: The EC50 value and 95% confidence intervals were calculated using probit analysis. 006599 Test 1 Percent Mortality/lmmobilization Nominal Test Concentration, mg/L 24-Hours 48-Hours Negative Control Solvent Control 100 180 320 560 1000 0 0 0 0 0 0 0 10 0 5 0 10 0 10 Test 2 Percent Mortality/lmmobilization Nominal Test Concentration, mg/L 2 4 -H o u rs 4 8 -H o u rs Negative Control Solvent Control 650 1000 1550 2400 3700 5600 8700 0 0 0 10 60 100 100 100 100 5 0 0 20 60 100 100 100 100 CONCLUSIONS The test sample 48-hour E C for Daphnia magna was determined to be greater than the test substance solubility limit. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. These studies lack analytical data for determination of the test substance concentration in the test solutions and determination of the sample purity. The tests should have been replicated. There was no description of stock solution preparation. Testing was also done well above the solubility limit of the test substance with the aid of acetone to enhance solubility. REFERENCES 006600 These studies were conducted by the 3M Company, Environmental Laboratory, 3M Lab Request Number 4197,1978. OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/12/00 006601 'For.m*67"4,7-11-A TECHNICAL REPORT SUMMARY Oatg Jan. 8, 1979 TO: TECHNICAL COMMUNICATIONS CENTER - 201-2CN y Im portant -- I f report it printed on both sides o f paper, send two copies to TCCJ division Environmental Laboratory Project Fate of Fluorochemical Project RaportTltia Aquatic Toxicity Studies: FM 3925 D. L. Bacon A uth or(t) A. N. Welter Notebook Reference 6aptNumbar 0535 ProjectNumber " "R9a9po7rt0N0um1b2er600 018 Em ploy* N u m b irlil 09362 No.ofPagIncludingCovtnhMt SECURITY ^ U Open (Company Confidanti!) U t Closed (Spadai Authorization) 3M CH EM IC AL w . REG ISTR Y W NawChemicals Reportad Yes a No KEYWORDS: (Select tarms from 3M -Thasaurus. Suggest othar applicable terms.) EE & PC Div. (Environmental Lab) Toxicity (Aquatic) ) CURRENT O BJECTIVE: To determine the acute 96 hour LCg0 value for bluegill sunfish (Lepomie maaroehiruB ) and the 48 hour LC-q value for Daphnia magna exposed to varying concentrations of PM 3925. REPORT ABSTRACT: (200-260 words) This abstract inform ation is distributed by the Technical Communications Center to alart 3M'ers to Company R A D . It is Company confidential material. The limited water solubility of FM 3925 prohibited a definitive determination of the aquatic toxicity of this test material. Both vertebrate and invertebrate LC__ values exceed the water solubility of FM 3925. 50 A. Prokop D. Ricker R. L. Bohon M. T. Elnabara wy A. Mendel E. A. Reiner S. K. Welsh Inform ation Liaison In itia ls: < g )I a- 006602 CO N CLU SIO N The limited water solubility oi FM 3925 prohibited a definitive determination of the aquatic toxicity of this test material. Both vertebrate and invertebrate LC,-ft values exceed the water solubility of FM 3925. INTRODUCTION The test substance FM 3925 is an off-white solid. Tests performed on this material evaluated its aquatic toxicity and the data obtained form the basis of this report. METHODS A. Aquatic Testing The test protocols utilized for these studies were modeled after that described by US/EPA (1975). B. Acclimation Procedure The bluegill sunfish (Lepomie macroehizns) were obtained from a private hatcheryt. Stock fish were held in a fiberglass holding tank filled with carbon-filtered well water at 14-15C. A daily photoperiod of 16 hours light and 8 hours dark with a 30-minute transition period was maintained throughout the acclimation and testing period. The fish were fed Tetra-Min daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated for 14 days prior to testing. An acute short-term (96-hour) static bioassay was performed on FM 3925. Carbon-filtered well water of known composition Was used as the diluent (Attachment 1). All glass aquaria (35x20x20 cm) containing 16 liters of diluent or diluent plus toxicant comprised a study chamber. Twenty bluegill sunfish, uniform in size, were tested at each chmical concentration. Test fish were randomly allocated to each test chamber within 30 minutes following toxicant addition. Bluegill sunfish experiments were conducted at 20-21 C. Mortality, temperature, dissolved oxygen level and pH of all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations relative to behavioral changs were similarly recorded when appropriate. These studies were performed in replicate. Organisms used in this study were considered to be generally healthy and free of disease. The Dapknia magna used in this study were obtained on April 19, 1978 from the Mogul Corp. Test protocol used was that described by USEPA (1975). 006603 + Dale Fattig Fish Hatchery, Brady, Nebraska ^ Pommorp iai vish fnnri of known composition 3 RESULTS The bluegill sunfish (Lepomia macrodhirua) used in this study had an average weight of 0.5 g and length of 4.0 cms. The loading factor for these studies was approximately 0.63 g/1, below the maximum recommended loading factor of 0.8 g/1'" . Test .temperatures ranged from 18 - 20C, pH varied from 7.8 - 8.2 pH units and oxygen saturation at 96 hours in the individual experiments were 34 and 56%. In this replicated study, LCg0 values were not calculated since these values would exceed the water solubility limits of FM 3925. (Attachment 2). The Jkxphnia magna study was conducted at a temperature of 19C., pH range of -.2 - 8.7 and a minimal 48 hour oxygen saturation of 79%. Projected LC-n values exceeded the water solubility of FM 3925. (Attachment 3). The data reported herein are based on studies performed by M. T. Elnabarawy*-and L. H. Pritchard. REFERENCES 1. US/EPA - Methods for Acute Toxicity Test with Fish Macro-invertebrates and Amphibrians, Ecological Research Series 660/3-75-009, 1975. 3 FORM _gF.qur.cr UQ. 4 3 6 2 . f S T A N b i N < a l MTt u tt i ATTACHMENT #1 > t >eSFR!PTl0l: A FtUeftEA W C L U ? / - V - 16-78 -7 -a s --7 f B -2 /-7 B ` i-u-nt 1 -6 7 -V * t - 21-19 4~Xi-76 * 1 <| H\6 0 Xf7 <o.) 7 .7 - <\ yyo XU 0 x t t <0.1 7.7 <\ y i* * 9 * ? 7 t u <0.1 Si < .)74 7*7 - - <1 i f i n <! y v r XX) 4l yoo 0 - lli *L i li i 0 9 -1 * TM i <00.1 - <1 yoo ** 0 % ! 114 7 . 7 <6./ <1 212 0 13 <0 .1 7*7 <0.1 Vfjr i n Rif, \ ) - nrdttiil C il a v a i i a d i t r ' . 006605 ... ... . . ______ L . . 11I V / ! ENVIRONMENTAL EN G IN EER IN G LABORATORY AQUATIC T O X ICIT Y DATA SHEET V.'* ATTACHANT 12 Typ Tt U-H0*r Shfhc N eteriel Tested ^ 3 \ 2 T __________ T **t OTRUil g t* X lQ AVf. wt. . r IT O Avt. 81m _ 4 C Test Solution Voli-- \A Liter/Teak No, Orfenisns por Eoo# *2.0 T i S ta rts d _ ^ jjfc S M lB ts Stnrted Doto Conplo<od_<V~ VT Q f Dilution Meter CerDon F ilte r - Noll Wotor f f n n . i t C .. B.O, M pp . pH ) Investitetor Y & . LC SO (95% Confidence U n it s ) _____ ___________ ' No. )rganisns Cane. isposed 1 Contro * *wrd 2 .0 2C> \3 22 / ?fc 20 2 .0 2o 6D 2 0 /CD 2 .0 24 Hm. D.O. No.Deed JH PW 0 tt 6.1 0 2? . 0 7.9 <o3 0 7.9 6.0 0 x% 0 2t 6.0 0 7. 6,0 4S llrs , D.O. No. Deed pH pp 0 tz 0 o 7* o 7.? 6.0 0 7.9 r.9 0 7 .* c ? 0 2S r .9 0 7.9 r. 11 72 Hrs. D.O. 96 Hrs. D.O. No.Deed pH bpu Mo.Peed nH noe 0 tz 6 0 2% 6 fr 1 7.9 6 .0 0 I 7 0 7.9 6 .7 \ 7.9 r ,r 2 7 9 Ct 2 2 7.9 .2 2 2? r.o "5 7.9 3 7.9 n 1 7.9 3 2 7.9 r .o Ave. Teno. Commts: .V C w rtv^ k u V o ^ f.d ,I .Ue \t*c _____ g & \? c .jC^VDfottntA ( M c H V ^ * ZmU'ttO_____________ ft, u i i Y W jW K c j) nlA- J , `il o I u,A J m 9 ml per J;u>r UsYOf) Wi el e-- l i n j kliVW... A ito ftln Inl^TLu ^--TrdV vrttf\hS ..ftwluoQH pffftfoVoftnl avAr,.. .SgVtUA aaeR W \ W l & W o W ^ ) / -- WvfvV^Yvflil \u*.Tfiftld A h W fflw/jiAVtahnrfo* //Wie./ L aos ') ^------ 006606 0\ ENVIRONMENTAL EN GIN EERIN G LABORATORY AQUATIC T O X ICIT Y DATA S IC H T ATTACHMENT #2 ) Typo Tet ^ lo V\v*. ^ A o X ,'. c Material Tatad P ~ C -- Test Or*mlm\LVK^ \ \ Avg. W t.O u tra n Avg. Sise H cn Ta*t Solution Voli-- IL Litors/Tank No. Organism par done V Q Tina Started *2*.% Date Started ^ \^ % Data Oonalatad - A - .22.T?g> Dilution Untor Cw> P tlt.r - D .U U .t.r tTun.\ ^ "c . . 0.0 .$-^ nun. Investigator LC SO (95% Confidence L*nit) ______________________ No. hrgaaism 24 Hr. D.O. 'Ca ixnosad No.Dead VM PM tro V Q hBusi V O *4 V O s VO 1 VO i-fk v a 0 is L 7rt .3 1 s -l i , c\ ... 5 ,\ Q *1 s.% O Vo t.v V Q _ _ -/ M LI vn V G / W r .v 49 Mrs. 0.0. No.Dead pH PM O 1.9 4.\ * * n.i M I 1 , 3 .U 0 S. 0 i i %<s s , o "lA V,3 i 72 I I . 0.0. No. Dead d -- \ It VA '3 \ Tfl *.*V n .t 3 . 0 v\ v*i o *iA 3-1 l <u 53 iw 96 llrs. D.O. No.Dead P<l PM \ H 3<* 3 19 3.C I 7 S .2.1 0 79 2.0 3 79 2 .? / 7.9 <Lb 3 7-f 2.7 I 29 .M - >/> cJ& SL________ W o tre. | Y ' c Lni_ * 2 .0 #C eats:S) _____ Si O i c a M t . _____________________ > ^ I V V ^ J ^ b o A -------- t--- L77:',L.\>sS^.'*__\12li-VQ-^Vf,r7.^r1J*u.l..2SLkliJ-tgLL^..A ^ - a ^ j . s L r i W c A .Va . f t 4. c , e V C L \ ' y . \ V t v V > \ f> VvA l V U y ' l ^ H ^ V k > ^ r ------------------------------------- BEST COPY AVAILABLE 006607 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TO XICITY DATA SHEET ATTACHMENT #3 typo T .,t M 1 n .'ttW flD . N t t .r l.l T .It.J "A Test Organisn^^fcfcKUAsJYCx>a$jth-- Av` wt`- 5 b i s s u Av- * -- rSc. c* Test Solution Voluno K x w l Inoset/ S S T No. Organises por dose O Tine Started Doto Started Poto Cosplotod Dilution Water Cotton Pilter - Noll Water (Tone. V \ C.. P.O.^ A ppe. pH ) investigatorV T ~ ^ v 'X A o .w k Vy\. LC 50 (05% Confidence U n its ) _________________ C one. N o. Irg a n is a s ix n o s e d HK C o n tro V <S fc a *A r-.\ fc fiW u S y \0 Q X . )%<} h \Q ^<0 ~*Sx 24 M rs . N o .D e e d J U Cl . . . . o 0 o 0.0 . ppn lin . 0 .0 , N o. Dead pH n o n 72 U r s . N o. Pond sH D .O . non 96 U rs . N o .D o a d D .O . o il jM M i t . ' s O n .* 1 7. ^ o T* n .V V StoO IO O O ' V '' v V O W i.t 7^T V- A Y J L . , I ? " K : _________ Coanents: Wo ________ \ V k . . . . ' ^ '^ o t V fV i ' ;e- ^TY\CXvA>a * v-y ~v t\lie*. \ \ ' J-l -a 1 ;__ \x> ``n V ^ 1 >\ 4>--_j^U v",V^ca.c,N7rI.C 7! V.;l*A l . BEST COPY AVAILABtt 006608 .> ENVIRONMENTAL ENGINEERING LABORATORY ATTACHMENT #3 , AQUATIC TO XICITY DATA SHEET Type T e s t^ V y V , M a te ria l Tested r Y V \ - Test O n m i e \^ N p W x o ^ Avg. W t.^ r g to to Avg. Test S olution VoluaelOOv^i No. O rg anism p e r t o e V O T im S ta rte d tL'.Od^Wy Date S ta rte d H ^ W * ^ Pe te C o w le to d H Dilution Water Carbon Filter - Woll Water fTaap.^flC.. 1 InvestigatoA^vX^U LC SO (9S% Confidence Linits) j3 f \ ^ | l ( \ \ < K \ - l T O ) aw y ' <* A Zone. No. >rgaism Exposed k Eontro W ... m *.0 24 H rs. D.O. 4S H rs. D.O. No.Dead j H p e e I No. Dead pH p w . 0 \ $.7 7.6 0 0 S.7 7.^1 72 lir a . 0 .0 . No.Poad nH non \| ! | 96 Hrs. No. Dead D.O. oil d m 1------- ^ O i_ o ? . r 7.2 '<Ltf6 TkCV ik O 2 12 Ar * r 7 6 \2 - 7 2 sv^ 0 %vO VQ V i-W Q rfcoo X.CL ^ 0 M S ts ' ^ 0 % u %<=>' k m $vO 0 . 0 'w YR, T e w . 1 19k *Z V - 7 ,0 -- 132 0 -- 20' j-- i /9 -5 i i _________ 1 ' ___ 1i 1i _____:: ,| i ka'xOw V V \ < v y > < x U \ m \ \ v V s \ e $ V 'Mr.*. ^ -- So-.a .vy% ^TY^i.^frl___-Q. ^ k s i S - A L -- 006609 1 RUM /*RQBIT.B20 f i u r s d a y * h a y lit 1979 13:34*31 V in TD -- ENVIRONMENTAL LAB. - DULUTH ***PROBIT ANALYSIS BIOASSAY DATA***" MO OF CURVES ? 1 NO. OF CONS. FOR CURVE 1 7 4 INPUT DATA-CONC.rNO. EXPOSED**.DEAD ? 690>200 ? 1000*20*4 ? 1330*20*12 ? 2400*20*20 L06 CONC. FRACTION RESPONDING 2.812913 0 1000 3 0.2 1330 3.190332 0.6 ^ 2400 3.380211 1 CHI-SO PROBABILITY IS > 0.03 FITTED G > ^ \ <vv4v*sr,W \ FRACTION.5, .0.003900374 0.1499294 0.6809147 0.9737903 0 n o 'OF ITERATIONS 6 INTERCEPT <A> -24.7904 SLOPE <B> 7.917881 ( 'TER06CNIETY FACTOR <H> * 1 LC--50 ________1331.882 93 PCT. LOUER CONF. LIMIT * 93 PCT. UPPER CONF. LIMIT * CHI SQ FOR LINEARITY CHI SO FOR PROBABILITY 1.61074 0.4469226 6 - 0.1348014 1193.331-- ____ ' 1333.081______ 006610