Document K6XaOy9n17oyN2LnropM4NYzX
/IRZX-055&
Summary PFOS Rat Cross-Fostering Study Numbers: 3M T-6295.13, Argus 418-014 ACnomalpytoiucanldR&eqLuoets:t 5P3F0O3S0)(Perfluorooctanesulfonate) - Lot 217, 98.4% pure (SMD C.A.S. Registry Number: 275-39-3 Study Title: Oral (Gavage) Cross-Fostering Study of PFOS in Rats Report Date: 23 July 1999 (in-life); 11 February 2000 (serum analysis) Study Year and GLP status: 1998 - 2000 and GLP study PPmfdeoremgosr/fciaknleulggedo/udrcrroaaoetyonss.ct:iwtnaGDeunroreeoesdsuiudnpilongsfsostoenhtdfaaerftfetefoeemrd(mPa4waFl2elOhedresSaant)ytshstbrh(oype2ur5difg/oeaghmrirlooytauoultoepmrgs)aealwwstitieneanrrtgteieuotbanaoapdtupaminonroidntnrxeitaishamtteteedar2dotee1sdmleydPalae7loyev0tssae.sdlossAafiyuoflstamfecort0ladmto.iaortTni1n.h.6ge, cwrccctAhaooorhtieonnnsespttetlrrrsriaeooot-irtftllltepso)ur;ddosrsoaatiswetcsmmwirioonbisesnnnl.rlte,g(eirn.toOtttoehelrngferad.anldcaitTstoaimtfvhyeneesertrosrrwcofefooaldilnuntaahdtrtcnortslodfaiuaoltt)ttnbire;roogeronstratbhort4tefeeuar,adrpoitlndsmeigtidatnrwemotgdrreusearppwamewhstheaasewidrscrmweehfdrioacaethlchumlaosoldlslukwie;tbiddtsnadee:enritlvsotidcivi,cfod1ertne0rsoredat,emrdmawoitnl-etpirttddleohneaaodsmeat.aeqddmsdudFaaiwsmodtliraiwontwmchniurtaasmhollsis(tblgtspaiee-rtolrftlorsseoosuirswtpsfpitrveseeofredrrmifonostmogerx, pfOiuxnpesldaocftofarctioeonlnetcrdotraloyannomdneitcr(erdoaatseycdooplfiitcbteierrtvsha)nluoluat tnuigosenad.ndfolrivcerorsssa-mfopslteesrinwge.reTchoelsleecttiesdsuferosmamspelleesctwedere AcIltdahnoancermataraddlestyadsitampoiitnnocisdao.n.nldlSiPLittnatuoaemgpbrtosthpsoreelweartcstehuorremoroeyfsdasamaf-nsomfsidorliskgspl.t,nieovseArseedesrlrdilutbsosmalliaemtp,mthfelpuirpavltslerue,esmrrseewaiawgcaneheonrdrtkeaeimclnoyofbeisrnltitoktsiarzc.ioseneslneacddrmaaenamptdnilnsedsgeacpnngootldloaltenlloecidtdtttsiheofrewnrso3eaomrMnnedtdoEhtbanewytvaloiii1trnte4oeexnrdopsmfofersnoedmtal EaOnnndvdilriatoytnesmros.efnlLtaaictlttaeLrtaiosbanomr2a1ptolearsnydwfo2err2ep, sopesorsouiblmeldea.fnuAdtulllriveseaarmnsapallmyesspiswl.eesrwe efrreozoebntaainndedshaitptpeerdmtforothme d3Mams
CC0005
dddrfsRt(heosheeeeiseooaaansudtttwpughhhltrllcnsssteeoos-(((b:dfnmddsyesipeeMnauauaattgtmhttpihehhlneeapsseginfntxdiiioannopbetlnhnlaooo8d1tr.sdhwe0uoygeRriiofeennwlefs1igdtitte21nawtuide1tglcioruaiohsletttita)netldtes.i.trtrepoptswTerCeu)sra.rerp)soienr.)oneaCd.sdttuTroresnTorerdn.ovedlhttiadrTuedvotaascahaelememldreddesdossdaseaciumXanXtcblmautsotTChrsiodXnrreofyeXedndateTiwrctftCerriefaeenoaeodtaitlcengtetptwpethdstruhudtooop(aple1pnstosf.upf6rfhthupeehatacmpdsheddtsuehsgdc33ah/face4kod/amod1/gdu1r91/rr7d1f2p6ers7a/ul/ou1(1aypm1(8bt6)1e.1-6s6d9fgeue%.r((xr2wmo1vp9)%u.iiao1.vtpp6lhs)%aeus%uplsprr)aeu)ewdspddp.puauueursapciptenahdgs lPcEmPoionFveliemrecOTocrrpStoxyrooisposfacuenototnhmIpmd1Iey.-e6iprpceopnmurlfreoapogusttshel/cmikedfofrgeopoll/rciiydmavvyateeotiyeortrfr.sndecthnhaiidetsoaenrrddaegoakvedynnesa-aomtoihlwnlesdantlhhrcaaaPeomtdFmnpeOiepulnwlSopcau-rrslben'ibavodlosuerdenrrndeigeerlsfaasnfttueseophmcdrutolbepwuienfnfderegrde(acl2tstins-vuot.ioernfrcTae3tohchdmXteiasf)pndrfootaoi.myunfE-ndpodldieenla-drcgrmoterrxilsaonaitsntdtoerpiedcmuaaptectesehs.ds.anTwghietehs tpvdPaOP1wnpao4FFndmo,lmOOluyclemSSoihvxtt,nateahbpevwtleeorrutioo)linseaotle,hgmndwddateesthalhtdetayeemhevassrepumeism2sonrls(ei1ooonn8dowlhf=eefap0e8sdtdtpe.r)hh0mpreesl8auuienmmesprspdeiueigprstmrsuthmeuahomtrmmefuPpfimFqporPaPulOriFlerFiawtOsStshnOtaeeS(ttlsraSidetsdkaca.vaaemto(teimnnnlo8ss=cn9dSaaeounn)p(nfr<ddptidrnm9ea00lg8imt..ti01Ptota5eohFnnprnesOpdpsf)psmtrS2trmohua.1mafdt)8veo.TydirtphnhTiptelsgehohmreceweps.oeupwemrbpoFuiagasopmoschllrakeetishctdntgsehosro.cepnoucoucufdiOnfpeanaitnntcdmrestoiedrlellaarwenuctwpvtiimtaoetaishitlnt.ahrsismot(Thotndohpefhaefldeem9aSy oaptawtedqPutFapurhorvxnuviununFereeogoeppepatattOruairrhrmreosetnsteevaneaSenstapafgeidgcdiiudnrstttrpraieoaeoerenadlnmdoeuntddtamrggaihnufcoome7taeddttmPo8tncu2varahafs8rFectnecommeaoipasOur(toorpnfatpralesrsnneSpgaetmgmx0hadt(aacmee.padofplentt0.emtoeafaonild(5eo(vredtysnlfnriisSsyeecsrnopftd=bldeer=opaentseao1piartrmsr4ximu4ekopse6-ntt)pdhsedmc.)eebsa-.oruatncrsfni-vpimLshtoefovdoIdeiueorarminseaadrodtpvndtstom1emptfetee(5hhiudorsadrdrna.eeeirasssurlansidrteeyiiumnyTmedntufdrslttresrwuamheeoi-tv2Ptlmtrrhed2aimtoki1sotFeiea1nPah.r)rsObmdtvFamtheaohrtocaPnOSrfsenenalaimudeeo)iuladSlsdr.knacvoytee2tee)ta2Ittls5,ver2oretdsel2ed4heaut,dx(sh)atoala8deh,prsupdettdsf8saeieoeoeapaen,malpddusnstmpmractwhouuldsecpefafdseomrthdomotPweatali(hbshseFotcdlothftwiieladorhOeeoargnituonrrcomnbnhllS-ap-ynmhiuoe,eddatvsutllttsoiaaverweodepettpaemnmxerrdwsnuaeisabrpssseartdapghteoseeethvrgeseaiehsducrisevaevdcerunerimrtiurneddmomafd2olutmoasgohel.Plsovtmsri5ienPweesktFi-sv4tls-ratfFstee(OiefaeootntOllpormrwlthsiySsuesposklt=Saiahriteemrsbeltvemsmsikrtl6ocvcraeae,y.)ve)iopmhilxrdtwTsaudtnhflcp.eoferehtachomofodoots9sfdeeorimssm,c8oinauotlsh3ompetrknripr4etraenahpefrfgfpterdmeeoirlopreodfoemdmnms
OGODOS
vtrieaamtedilkd.ams. These analyses clearly demonstrate that exposure occurs both in utero and fePepC6rxfu2uoofpl9pemnmos5ccst.olecs9uunxa)rs(anpeiiwrno.oybncaseIresssne:dueaa-xrusdRfrpteaaeeeodcmsrdsutopaesul.netdecrtxeootdpbofxoyppisssuiupuogppurmnpeeisesfxuoispcrdf)voaoPbnisevFuetsaiOrlnlneeSgoovibtenp(lsdarsueepartosmpeveferenoaPtdtrrFeaiitOannnotdSettahhdnfefeforaeonPttcmeFat1ewO.rd6i-enSbsmbuouyrglmtate/etkroxrpogfpeu/poedeprsfxa.fuopyedrToc)eutshcscuteaotronieponPnrraFotenhOsdsdtueuuSlcvddttiiayiiannom(nmuslitfeiveorelroekfoTmr.reNba2aaPdhtteoanndd0uoiFlOkxsdhdd0rawOpeid,ati0iTcneotetSen.3iiiEvsntooc)ftTyiedoneon:toarhiu,rat,ofsobeorlAatpeniatuenparootnlmrruhtitghbsahnhecmpsaejlorpoeyirrcoapbuermcmshaduuelggitnaedsipggapnhvndicshnlaaiteie;osisasnntstopaiktn2eacnsinrmoi)cclysoenayufyugpmtle/nsaetnlctiahnppdoeisaonPcidhsehrsnmdeoFraasoctssfrrOhptpltymfmtuaaPrulrSleocdneaFadaittuvicdncceOycaeigedioo.otssnrSihakkeno,glrw,Riliacnegunyncteoe:e.erheetnrpfidsatete1nTtoiitetts)rccaeihrfehuaot,ttietieodnssnnisoftaodpe.eaunbaftneocmirad.etrIreesth.styntepstTateen-ea,lTkileshxnrtsnimethisfedpuoaupneeieldh-nllspsceakiayetdeposotfn,vceeeftentceoeaerbdrhxoseinnncernntadbmtdtdetaaoimtyeanletpttmsealodirAltvabnleuimdceseletutdekthhedlesegafy-eaniffcruptoterotihehpsanroacrxaetioocntnnnartsdts3aadheemisut1llosiedrhiysc,tibisfueizzpniesl2ccnPeeeoets0eudsgFsesedd0tmd.incOut0itgioidshvdee.TSlopnpeacsiinwaeodnohIabswnsdiaoso;ihninsiltflttaiiilhstwobsly.efmno;eyodrofeyoff
0G0C07
Summary PFOS Rat Cross-Fostering Study Numbers: 3MT-6295.13, Argus 418-014 CAonmalpytoiucanldR&eqLuoets:t P53F0O3S0)(Perfluorooctanesulfonate) - Lot 217, 98.4% pure (SMD C.A.S. Registry Number: 275-39-3 Study Title: Oral (Gavage) Cross-Fostering Study of PFOS in Rats Report Date: 23 July 1999 (in-life); 11 February 2000 (serum analysis) Study Year and GLP status: 1998 - 2000 and GLP study PPfdmeoremgors/fciaklneulggdeo/udcrrroaaoetyonss.ct:iwtnGaDuenroreeoesdsuiudnpilongsfsotosenhtdfaaefrtfeetfoeemrd(mPa4waFl2elOhedrseSaant)tyshstbrh(oyp2eur5difg/oeaghmriroloytauoultoepmrgs)aealwwstitieenanrrtgteieuotbanaoapdtuapminonrodintnrxeitaihsamttetedear2dotee1sdmleydPalae7olyev0tssae.dslossaAfiyuoflsatmfecort0ladmto.ioartnTi1n.h.6ge, ctrcccwAhaooorhtieonnnsespttetlrsrrraieooo-tirtftlltlepso)urd;dosrsoataiswetcmwsmiroionibessnnn.lrlte,ge(irn.tOottotheelrnfegard.anldcaitTstoaimtfvhyeneesertrosrrwcofefoaoldilnuntaahdtrtcnortslodfaiuaoltt)ttnbire;roogerontsratbhort4tfeeeuar,darpoitlndsmeigitadnrtwemotgdrreusearppwaemhwstheaasewidrscrmweehfdroiacaethlchumlaosoldlulskwie;tbiddtsndaee:enritlvsotidcivic,fode1rtner0soreadte,mrdmawoitnl-eptirttddleohneaaosdamet.aedqdmsdduFaiawmsodtliraiwontmwcnhiurtaasomhllsis(tblgsptaie-erotlfrtolrsoesosuirswtpfspitrveseeofredrrmfionostmrogex, pfOiuxnpesldaocftofarctioeonlnetcrdotraloyannomdneitcr(erdoaatseycdooplfiitcbteierrtvshan)luoluat tnuigosenad.ndfolrivceror sssa-mfopslteesriwnge.reTchoelsleecttiesdsuferosmamspelleesctwedere ctlAIdahnoancermtaaraddlestyasdiatopmiintnocisda.on.nldlSiPLittnatuoaemgpbrtoshtpsoreelweartcstehuorremooreyfsdasamaf-snomfsidorliskgsp.lt,nieovseArseedesrlrdilutbsosmlaliaemtp,mthfelpuirpavltsrleue,emsrrseewaiawgcaneheonrdrtkeaeimclnoyofebirsnltitoktisarzc.isoensenleacddmraaenapmtdinlnesdsgeacpnngootldlaoltenllociedttdttisheorfewnrso3eaomMrnnedtdoEhtbanewytvaloiii1trnte4oeexnrdopsmfofersnoedmtal EaOnnndvdilriatoytnesmros.efnlLtaaictlttaeLrtaiosbanomr2a1ptolaersnydwfo2er2re,psopesorsouiblmeldea.fnuAdtulllriveseaarmnsapallmyesspiswl.eesrwe efrreozoebntaainndedshaitptpeerdmtforothme d3Mams
CC0C08
rddd(sRfthesoheeeeiseoaaoansudtttwupghhhlltrlcnsssteeoos-(((:bndfmddsyespieeMnuaauaatgttmhttpeihlhhneaepessignfntxdoiiiannpobetlnlahnooo8d1tr.sdhw0euoygeRriiofenenwlesfi1gtdtiet2a1ntwuide1tglciruoaiholtesttitnta)edltes.it.rtreppotwsTreCeu)srearr.po)srine.)oneaCd.sdttTurroesnTroernd.ovehdlttiadrTeudvotasacahaelemedmrleddesdossdasecaiuXmanXctblmautstoTrChisodXnrreofyXedendteTaiwrtcftCrerifeaeneaeoodtiatclengtetptpwhtedstruhdutooo(papel1pnstosf.puf6rfhtheupehatacmdpshedtduseshgdc33ah/face4ko/daom1d/ugdr191/rr7df12p6ers7a/ul/ou1a(1ypm1(b8t6)1e.1-6s6d9fgeue%r.((xr2mwo1vp9)%u.iiao1.tvpp6hls)%aue%suplsrpr)aeu)ewdspddp.puaueuursapciptneahdgs lcEPPomionFveliemrecOTocrrptSxoyrooisposfaucenototnhmIpmd1Iey-.e6iprpceopnumrlforeapogusttshel/cmikedfofrgeopoll/rciyidmavvyateeotiyeortrfr.sndecthnaihidetsoaenrrdadegoakvdeynnesa-aomtohilwnlesdantlhhrcaaaPeomtdFmnpeOiepulnwlSopcaur-rsleb'nibavodlosuerednrnrdeigeerlsfaasnfttueseophmcdrutolbepwiuenfnfedregrde(ac2lstntisuvot.iorenfrcTa3etohchdmXteiasf)pndrfootaoi.myunfE-ndpolddieenla-drcgrmoterrxilsaonaitsntdtoepriedmcuaapctetseehs.ds.anTwghietehs dPpPatvO1wnapoFF4nmd,olmOOluyclmeSSoihvxtt,nateahbpevwtleeorrutioo)linseaotle,hgmndwddatetsehalhtdetayeemhevassrepmuiesm2sonrls(ieIooonnSdowlhf=eefap0e8sdttdpe.rh)h0pmreels8auuienmmesprspdeiueigprstmrsuthmeuahomtrmmefuPfpimpFqorPaPlurOiFleFriawtsOstSOhntaeeS(ttSlsraidetsd.kacavaaemot(teimnnnlo8ss=cn9d8aaeou)np(nnfr<ddptidrnm9e0a0lg8imt..t0i1Ptota5eohFnnprnesOpdpsf)spmtrS2tromhua.1madtf)8veoT.ydirtphnhTiptelsgehohmrecewespo.eupwemrbpoFuiagasopmoschllraeketishctdtngsehosro.ceponucouucfdOinfpeanaitnntcdrmesotiedrelllaarenwucwtpvtimtiaoeatishitlnt.arhsismot(Tohtndohpefhaefldeme9aSyotawpaqtudetPFpuahrorxnvuvuniunFreeeogpeoepatpttraOauirrrheromnesstteevnaaneSestapafiegcgdduiidnrstttrrpaieoereaoendlandmounetddatmaggahinfciooe7tmadedmtPto8tnucv2raahaf8FrsecnteocmmeoapiasuOrtor(ponaftrpalerssnneapStmgeg0mxhtda(acamee.padoflpetn0t.emtafooenaild5(eorv(edtsnylfrniiyssSeencrsopfdtb=ldeer=onaeptseao1iprastrmr4xium4epsko-6tetn)pedhsmd)bece.sar-.oauctrnsfnvp-imLhsioteofdvoIdeiuoerramisnaeadrotdpnvdtstmo1mepftetee(5hduiohrsdarrdnae.eesarusisrlansrdtieyiieumneTnmytfdundslttrrewsameheiiotv-P2tmtrlrhed2aimtoki1sotFiean1Peahr.)rOsbvtdmFatmheaohartocPnOSrfensnealaimudeeo)iuladSldsrv.nakcoytee2teate2)Ittls5,ve2rroeetsdl2d4heaeut,xd(s)hoatal8daeph,srpudtetsdf8saeiooeepaaeen,malpdudstmnsprmacwtuhuoldecpsefadfseromhdtoomPtwatealsi(hbhseFotcdlothwftieialdroehOoearngiturnoocmrnbnhllSa-p-nymhuio,ededtavsutlttlsioaervaewdoeepttpanemxmerrdwsenuiasabrpsressadtpgahteoseeertvhegseaiehsrducisveaedcevrunerimrtiurdndmeomadf2lotumoasgoehl.Plosvtrsm5iienwPeeskst-iFv4l-rttastfFseie(efOaooetntolrplOmrwtlhsiySsuessokplt=aSihtarieermsbeltemvsmiksrtl6ocrcvaaee,y.)vi)oehmpirxldtTwasudnhtfcpl.eofrehetcahomooftodo9ssfeedriomssm,c8noiatousl3hoemptrknripr4treanehpaefrfgfpertdomeerliropdefeoomdmnms
CC0003
vtrieaamtedilkd.ams. These analyses clearly demonstrate that exposure occurs both in utero and eePpfC6rfx2uuoofp9lpemnmo5cscst.olce9usunax)rs(anpeiiwrno.obyncaseIresssne:duea-arxusdRrfpteaeaeeodcsmrdstuopaeuslndt.eectrxeotodpbofxoyppsissuiupugoppurnmepeisfesxuoispcrdf)avooPbnisevFuetsaiOrlnlneeSgovoibtenp(lsdarsuepaertosmpeveferenaotPdtrrFeaiitnaOnnotdSettahhdnfefeforaenPottcmeFat1ewO.rd6i-enSbsmbuouyrglmatte/etkroxrpogpfeu/opedepsfrxa.fuopeyrdToc)eutshcsuctaeotronieponPnrraFtoenhsOsddtueuuSlcvddttiiayiiannom(nmusliftevieorelreofkorTm.eb2rPdhaNtaataoennd0duoiFkOlxsdhd0drwaOpeid,at0iiTcnoeteteSn.3iiiEvsnotoc)ftTyiedoneo:ntoarhiu,rato,fsobeorAlatpeniatuenaprootlnrmrhuttighbsahhnecmpsaejlorpoeiyrrcoapbuecrmmshaduuelgtgineadispggpanhvndicshnlaaiteei;ossiannststopaiknt2eacnsnirmoi)clycseonauyfyugpmtle/nsaetnltcinahdppeoisaoncPidsehhrsnmoedrFaasoctssfrrOhtpplytftmmuaParulrSledocneaFadaittiuvcdncceycaOeigedioo.otssnriShakkeno,glrw,Riliacneugnyncteoe:e.reheetnrpifdsaette1nTtiiotetst)rcceaihfrehuaot,titeiteodnsnsnsoiftadoep.eaufnbatencimoard.etrIreesth.styntpestatTee-aen,lTkileshxnrstnimethsifepduouapneeiehdl-nsllpscekaiaytedpeosotfnv,ceeeftecnetoeaebdrrhxoseinnncenrnatbdmddttteaoaimtyelaneptttmseoalidrAtlavbnlueimdceseletutdekhthdleesegafy-eaniffcruptotreotihehpsanroacrxeatoicontnnnatrstds3adhaeemisut1llsoiedrhiysc,itbisufezipznies2lccnPeeoeets0eudgssFseed0dtmd.icnOutit0gioishddveeT.loSpnepacsiinweaodnhoIasbwnsdaioso;hniinsiltftaltiiilhstowbsly.femnoe;yodrofeyoff
o o o o io
PROTOCOL. 410 008: COMBINED ORAL (GAVAGE) FERTILITY, DEVELOPMENTAL AND PER INATAL/POSTNATAL REPRODUCTION TOXICITY STUDY OF PFOS IN RATS (SPONSOR'S STUDY NUMBER: 6295.9)
TABLE E20 (PAGE 1): 1,ITTER OBSERVATIONS (NATURALLY DELIVERED PUPS) - SUMMARY F2 GENERATION LITTERS
MATERNAL DOSAGE GROUP MATERNAL DOSAGE (MG/KG/DAY)
I 0 (VEHICLE)
1] 0.1
III 0.4
DELIVERED LITTERS WITH
ONE OR MORE LIVEBORN PUPS N
22
21
24
PUPS DELIVERED (TOTAL)
N
MEAN+S.D .
316 14.4 +_ 3.5
325 15.5 : 2.5
357 14.9 t 2.6
LIVEBORN
MEANfS.D. N(\)
14.1 3.5 3101 98.1)
15.1 2.4 318( 97.8)
14.3 + 2.5 344( 96.4)
STILLBORN
MEAN+S.D. N (%)
0.3 0.6 6( 1.9)
0.3 1.1 7( 2.2)
0.5 + 0.9 13( 3.6)
PUPS FOUND DEAD OR PRESUMED CANNIBALIZED
DAY 1 DAYS 2- 4 DAYS 5- 7 DAYS 8-14 DAYS 15-21
N / N (%) N/N(t) N / N (%) N / N (%) N/N(%)
2/3101 7/3081 0/171( 0/1711 0/17K
0.6) 2.3) 0.0) 0.0) 0.0)
2/318( 2/3161 2/1671 1/1651 0/164(
0.6) 0.6) 1.2) 0.6) 0.0)
2/344( 11/3421
5/191 ( 1/1781 0/1771
0.6) 3.2) 2.6) 0.6)a 0.0)a
VIABILITY INDEX b
% N/N
97.1 301/310
98.7 314/318
96.2 331/344
LACTATION INDEX c
% N/N
100.0 171/171
98.2 164/167
96.7 177/183
DAY(S) = DAY(S) POSTPARTUM a. Excludes values for litter 13257; the dam was found dead on day 10 of lactation; eight pups were sacrificed. b. Number of live pups on day 4 (preculling) postpartum/number of liveborn pups on day 1 postpartum. c. Number of live pups on day 21 (weaning) postpartum/number of live pups on day 4 (postculling) postpartum.
418-008:PAGE E-23
0000*
PROTOCOL 418-008: COMBINED ORAL (GAVAGE) FERTILITY, DEVELOPMENTAL AND PER INATAL/POSTNATAL REPRODUCTION TOXICITY STUDY OF PFOS IN RATS (SPONSOR'S STUDY NUMBER: 6295.9)
TABLE E20 (PAGE 2): LITTER OBSERVATIONS (NATURALLY DELIVERED PUPS) - SUMMARY - F2 GENERATION LITTERS
MATERNAL DOSAGE GROUP MATERNAL DOSAGE (MG/KG/DAY)
I 0 (VEHICLE)
II 0.1
III 0.4
DELIVERED LITTERS WITH
ONE OR MORE LIVEBORN PUPS
N
22
21
24
SURVIVING PUPS/LITTER a
DAY 1 b
MEAN+S.D.
14.1 + 3.5
15.1 + 2.4
14.3 2.5
DAY 4 PRECULLING
MEAN+S.D.
13.7 + 3.3
15.0 + 2.8
13.8 + 2.4
DAY .4 POSTCULLING MEAN+S.D.
7.8 + 0.8
8.0 0.2
8.0 + 0.2
DAY 7
MEAN+S.D .
7.8 + 0.8
7.8 + 0.5
7.8 + 0.7
DAY 14
DAY 21
PERCENT MALE PUPS PER NUMBER OF PUPS SEXED
MEAN+S.D. MEAN+S.D.
7.8 + 0.8 7.8 + 0.8
7.8 + 0.7 7.8 + 0.7
7.7 + 0.8
I 24 ]C 7.7 + 0.8
( 24 ]c
DAY 1 b
MEAN+S.D.
47.9 + 15.2
52.1 + 10.9
47.0 + 13.3
DAY 4 PRECULLING
MEAN+S.D.
47.9 + 15.1
51.5 + 11.1
46.6 + 13.4
DAY 4 POSTCULLING DAY 7
MEAN+S.D. MEAN+S.D.
47.0 + 10.0 47.0 *_ 10.0
49.6 + 1.6 48.9 + 3.9
49.1 + 5.7 48.7 + 6.4
DAY 14 DAY 21
MEAN+S.D. MEAN+S.D.
47.0 + 10.0 47.0 + 10.0
49.2 + 2.6 49.2 2.6
48.3 + 6.2 I 24 )c
48.3 + 6.2 [ 24 )c
DAY DAY POSTPARTUM ( | = NUMBER OF VALUES AVERAGED a. Average number of live pups per litter, including litters with no surviving pups. b. Includes pups born alive, found dead day 1 postpartum. c. Excludes values for litter 13257; the dam was found dead on day 10 of lactation; eight pups were sacrificed.
418-008:PAGE E-24
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PROTOCOL 418-OOB: COMBINED ORAL (GAVAGE) FERTILITY. DEVELOPMENTAL AND PERI NATAL/POSTNATAL REPRODUCTION TOXICITY STUDY OF PFOS IN RATS (SPONSOR'S STUDY NUMBER: 6295.9)
TABLE E20 (PAGE 3): LITTER OBSERVATIONS (NATURALLY DELIVERED PUPS) - SUMMARY - F2 GENERATION LITTERS
MATBKNAI* DOSAGK GHOUl* MATERNAL DOSAGE (MG/KG/DAY)
i 0 (VEHICLE)
1) 0.1
hi
0.4
DELIVERED LITTERS WITH
ONE OR MORE LIVEBORN PUPS
N
22
21
24
LIVE LITTER SIZE AT WEIGHING
DAY 1
MEAN+S.D.
14.0 4 3.5
15.0 4 2.6
14.2 + 2.5
DAY 4 PRECULLING
MEAN*S.D .
13.7 4 3.3
15.0 4 2.8
13.8 + 2.4
DAY 4 POSTCULLING MEAN+S.D .
7.8 4 0.8
8.0 + 0.2
7.9 + 0.3
DAY 7
MEAN+S.D.
7.8 + 0.8
7.8 4 0.5
7.8 + 0.7
DAY 14
MEAN +S .D .
DAY 21
MEAN+S.D.
PUP WE1GHT/LITTER (GRAMS)
7.8 0.8 7.8 0.8
7.8 4 0.7 7.8 4 0.7
7.7 + 0.8 { 23] a
7.7 + 0.8 ( 23]a
DAY 1
MEAN+S.D.
6.3 4 0.8
6.1 + 0.5
6.2 + 0.5
DAY 4 PRECULLING
MEAN+S.D .
8.7 4 1.6
8.2 4 1.0
8.0 + 1.3
DAY 4 POSTCULLING MEAN+S.D .
8.8 4 1.6
8.3 4 1.0
8.0 + 1.3
DAY 7
MEAN+S.D.
14.7 4 2.4
13.9 + 2.2
12.8 + 2.6*
DAY 14 DAY 21
MEAN+S.D . MEAN+S.D.
32.0 4 3.5 50.1 4 5.1
31.8 4 3.1 49.2 4 5.0
28.9 + 4.7** ( 23 )a
46.5 + 6.3 [ 23 ]a
DAY DAY POSTPARTUM ( ) * NUMBER OF VALUES AVERAGED a. Excludes values for litter 13257; the dam was found dead on day 10 of lactation; eight pups were sacrificed. * Significantly different from the vehicle control group value (p<0.05). ** Significantly different from the vehicle control group value (p<0.01).
418-008:PAGE E-25
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