Document Jrx7B8YrG9b9X22kmNOaBL79e

2 mm P N F J DfeNT ;a i 11. bumary of Mortality Among Group of Animal i.'V.bjeotod to Aerosol Formed by Dropping Tydraul F9i Aroolor 12U8 or Triorosyl Phosphate Into a Hooted Inoonol Tuba. 12 Inoldonoo of Mortality in Relation to Matorlal and Speoios Regardless of the Toriporature of the Inoonol. 13. inoldorioe of Mortality in Halation to Material, Rpooiea, and Temperature of Inconel. ll.|.. The T o x ic ity o f T y d ra u l r - 9 When -n in ta in o d Contlnuouely for Twenty-four Hour in Contoot With the Skin of Rabbit by the .'leave iothcxl of xJrniBo, Woodard and Calvary. lfi. The Toxiolty of Aroolor 12!j.Q Whon liaintainad Continuously for Twenty-four Hour in Contaot with the dkin of Rabbit* by the ftleeve Method of Oraize, Woodard and Calvary. 16. The Toxiolty of Trioreeyl ihoephate When Maintained Continuously for Twonty-four Hours In Contaot With the .kin of Rabbit by the Mloovo Method of Dralee Woodard and Calvery. 17. The Toxiolty of Gargoyle DTK When Maintained Continuously for Twenty-four Houre in Contaot With tho Dlcin of Rabbits by the r.loevo Method of oraize, ./oodard and Calvory. 10, 3ur.riarized Data on tho limnedlate Toxicity of hydraul P-9* Aroolor 12lj.8, Trioreeyl phosphate and Gargoyle DTC When uintained Upon the :.'ltin of Habbita Aocording to tlio hloove Method of braize, v.'oodard and Calvary. NEV 016*73 715725 CONFIDENTIA 1 Koated. Well From Whioh Pydraul F-9 Waa Evcporatod Into Air Streams. 2. Tower# From Whloh Liquid# are Volatilised. 3 [equipment for tho Donoration of the Docewpo#ltion Products of Hydraulio Flulda at High Toiqporature# if. Abeorption Train lor Collection of Carbon Honoxide From Air* j>. standardisation Curve for the Determination of Pydraul. 6. Standardisation Curve for the Determination of Aroolor 12if8. 7* standardisation Curve for the Determination of Irioreeyl Phosphate. 8. standardisation Curve for the Determination of Sodiwf Chloride. 9. Standardisation Curve for tho Determination of ::eta-Cre#ol. 10. Standardisation Curve for the Determination of FaraCreeol. 11, Schematic Arrangement of Equipment shown in Figure 3. NEV 016474 715726 p Q N -f1'lU'ri5'd-'NK)'--T JX l r. The Toxiolty of the Fogs Formed by Dropping Pydraul F-9. Aroolor 1245 and Trloresyl Phosphate, Upon the Surfaoe of a Heated Inoonel (Projeot 49) Soope The purposes of this investigation were to determine the immediate toxioity of Pydraul F-9 and two other liydraulio fluids, Aroolor 1245 and trioresyl phosphate, (1) when vaporized at moderately elevated temperatures and (2) when inhaled, by animals of several speoies, as fogs formed by dropping them upon Inoonel, maintained at temperatures of approximately 1250* and 1050* P. (677* and 566 0,), these temperatures being, respectively, somewhat above and somewhat below the fire point. A supplementary statement oonoerning ihe immediate toxioity of these materials when maintained in oontaot with the skin of rabbits is inoluded at the end of this report. ConolUBlona 016*75 1. Animals of several speoies survived without signs of lntoxioatlon when exposed to air laden with Pydraul F-9, Aroolor 124$ or trioresyl phosphate for 7 hours per 715727 com FId ^ N tiA l 2 day on each of several days, ihe concentration of the motorials in the cdr and the numbers of day of exposure yore as follow fydraul F-9, 3 *8^mloro<.\ram* por liter, 5 d a y s } Aroolor 12/48, U3 l miorotrams per lltor, '4 days; nnd triororyl phosphate, 01,5 nloro^rams per liter, G daya. , jho inhalation of vapors of iydrnul 1'-9 , Avoclor end tricrosyl piiOi-phate formed at noderntely olovatod room to..poraturos induced minor chan*.; in the Iuj-jj.s of the r<.nl.nrla, 1 .expiratory exposure to either Fyursul >9 or trlcroeyl phosphate induood vodorste increases In uhe 'u-bore of histiocytes within the lunj; parenchyma and the air parro ex of oo.tp, whllo correspond itv, exposure of othor specie to either fydraul F-9 or Aroolor 12i|.0 induood .Interstitial pneumonitis rnd apparently contributed to the severity of this lo.Ion ns it is observed., not infrequently, in control animals*. Intorcurront infoction, unrelated to the experimental prooedui'o, obsoured interpretation of the morbid changes in the tissues of a rulnoa pig and 2 rabbit exposed' to the vapor of trioroayl phosphate, one of whloh died day after the last poriod of exposure, i'he vicoora of the rate and rdoe v;ere normal, Kabbit i'907> the only animal of the roup exposed to Aroolor 121(8 from whloh tissues wore subjeoted to mlorosooplo examination, had fatty degeneration and neorosla of the NEV 016476 715728 v mr r v T ~n _. . . ___c , 3 hepatio parenohyaa. Other animals that wore exposed to vapors from Pydraul F-9* Aroolor 12W or trioresyl phos phate exhibited no histologio alterations of the visoera, aside from the lung, whioh oould be asoribed to the toxlo notion of the materials under investigation. 3 . The fogs of these materials arising from oontaot with Inoonel at either 1250 or 1050* F, were immediately irritating to the muoous membranes of animals. Exposure for 20 minutes to the fog of Aroolor 124d induoed labored respiration, but in the oase of the other materials thia booane evident only after exposure for almost an hour. All animals survived when exposed for 15 or 20 minutes to the fogs formed from Pydraul F-9 or trioresyl phosphate, at either 10500 or 1250 F., but exposure for corresponding periods of time to the fog from Aroolor 1 2 resulted fatally. In general, guinea pigs and mioe died more promptly than rats and rabbits. 5 . Guinea pigs and mioe suffered greater over-all mortality than did rats and rabbits when exposed to the fogs formed from any one of the three materials. Aroolor 12^6 was more toxio than either trioresyl phosphate or Pydraul F-9 irrespeotive of the speoiee of animals by which toxioity was tested. 6. The fog of Aroolor 124d formed by oontaot with Inoonel at 1250* F. was more toxio than that formed when NEV 016*77 715729 m the temperature v m 105>Q P. The fog of Fydreul F-9 appeared to be slightly more toxio wlion generated at the higher temperature, but the oonveree vae true of fogs from trloreeyl phosphate. y. halving the rate of dollvory of the liquid upon tho heated natal tendod to lesson the mortality Induced by Inhalation of a resulting fog, but this was not true in all Instanoe. 0. Mlcrosoopio examination of tissues from animals that diod as a result of exposure to the fogs of iydreul ?-9, Aroolor lFJfO and trioronyl phosphate rovealod pulmo nary lesions characterised by Intra-alveolar edema and hemorrhage, diffuse hyperemia, emphysema and oxtonelva dopoeltlon of carbon in tho hronohi. There was also pei'oncbymatous degeneration of the llvov, kidney end broirw `Tissues from tho animals that survived revealed only pul.onary looione manifested by interstitial pneulonitlo and .infiltration of oarbon-lnden histiooytoa throughout tho alveoli and bronchi. Tho lungs of animals that wox*e exposed for the ahortost periods wove nomal, as wore those killed 28 days or longer after the last ' period of exposure. 9* 'The minimum lethal dose of undiluted Fydraul F-9, when maintained in conteot with the lntaot skin of rabbits aooordlng to the 2i|.-hour sleeve method of Dralse, NV 016476 715730 ~5- L v.'oodard and Calvary, was greater than 3*6 ml* per Kg. and loss than 6.0 ml. per kg., of body weight. The correspond ing value for triorasyl phosphate was between 1.6 and 2,5 ml. per kg* Aroolor 1246 was not lethal in the dosage of 9.4- ml. per kg. Pydraul F-9 and Aroolor 124-6 are more toxio if applied to the abraded skin. Materials Pydraul F-9 is a clear, light amber, oily liquid whioh has a not unpleasant sweet odor. Its viscosity at 100 F, and 210 F., respectively, is 56.0 and 6.0 Centistokes; its speoifio gravity at 25*/25* 0, is 1.266, its pour point +5* F. Triorasyl phosphate is a colorless, odorless liquid, the visoosity of whioh at 0 and 25* 0., respectively, is 1256.14 and 66.6 Centistokes. Its molecular weight is, 366.4, its speoifio gravity at 25/25 0 ., 1 .162, its flash point 470* F. (243* C.), it crystallization point is less than -35* c a*1*5 ibe boiling point 420* C. The sample used was made from oresyllo aoid in whioh there was less than 3 par oent of the ortho Isomer. Aroolor 1246 is a straw-oolored liquid whioh has a slightly sweet odor. It is more vleoous than trioresyl phosphate. Aroolor 1246 is biphenyl ohlorinated to the NEV 016^79 715731 extent of lj.8 per oent. Its molecular weight is 192, and ita density la l.ljii g. par ml. at 30 c (06 F.) and 1.19 & per ml. at 300 C. (572 F.). .Its viscosity ranges from 112 oentlpoise* at 30 0. to O.I4.7 at 300 C.# and lfc distills between 3^0 and 375 0, its vapor pres sure being 0.00037 wa., 0.16 r"i.# 2.9 rim. and 360.0 mm. of mercury at 370 C., 100 0. 150 C. and 300 C.# rospoolively# according to hoArdle# Carrett tmd benignu* (Ir.d. .'ng. Chon. Ii.l)13Ul 19lp9> Those authors have quotod the National Board of Fire Underwriters to the ofl'eot that "decomposition of the prodxiot (Aroolor 12^8) was not appreciable at temperatures below if00 C. (752 F.) but booame increasingly apparent at higher temperafcures,, The oo^pound has a flash point of 193 to 196 0, but no fire point In the Cleveland open cup, 'bon oifchor iydroul F-9 or Aroolor 12ij.8 was drop.od into an Inconel tube maintained at approximately 1250 F. (677 6.) the liquid burned momentarily with a bright yollow-white or orange-whlto flame# and then burst into a dense cloud of black smoke. tfuoh glossy oarbon was dopositod on the sides of the manifold loading to the ohambor# which rapidly became filled with dense grey smoke. This soon turned jot black# apparently booause of the formation of flakes of carbon as soot. At 1050 F. (566 C.)# these liquids smouldered and burst into a NEV 016480 715732 >.r O N fi- 7 f0 N dense grey-white fog, in which wore anall amount of car bon soot In all instance tho odor v.ao acrid and pungent, vhon dropped into the inconel tube hoated to either 120 or 100 F, tricreayl phosphate gave rise to a character istic phenolic or oropylio odor and behaved aa did tho other two ntorlnlfl when di'oppod upon raetnl at tro highor to>porat'.'.re. At temperature below 102!? tricroayl phooyhato oppeerod to s*.nouldor wlthout flaming Kxporlm.or.tnl Method r.quipnont, i:he ani-rialo wore oonf J.nod in a 223-11 tor cylindrical i.tool ckn Oor coated on tho inside aurfaoe with a oaked plactio. Jrrteea a heavy fog vaa prooent tho bo. av lor of tho aol ala could bo obeervod through a aheet of clcar pliable plastic no -brane, which ao&led the oc.orwino open front end of the olinrbor. An interposed oavy wiro-neoh soroen prevented the onimnla from breaking tbo plan tic membrane The contaminated air entered the ehfu.Lor near tho top of tho roar wall on which we* mounted a circulating fan, Affluent air wea wltlvdrawn through an outlet located toward tho front of the lower pert of the chamber by ..alntalning a alight negative pressure* Volatilisation of the `Material Without Decomposition or tho volatilisation of either trioresyl phosphate or Aroolor 121(8 two flat-bottomed cylindrical bubbling towera NEV 0164.fi! 715733 C O N F I D*E* N T I A L 8 (2.9l+w x 13") In parallel (Kigure 2) word on^loyed, * fritted glass thiwble (0.9V* * 3*75*) being immersed in the liquid eontalned in eaoh* These towers were kept in e bftth of Hujol maintained et 109 0* Air parsed through thd two bubbling towers at rates of 5*0 and 6*3 litors per minute, respectively, as measured by a rotamotor in oaoh line the streams merging in & glass tube leading to a manifold immersed in a oooling bath before entering the chamber. At the etart of the experiment $$0 milliliters of either tricresyl phosphate or Aroolor 121+8 were plnood in oeoh of the two bubbling towers* In the case of tri- orosyl phosphate, it was found that the oonoentration of vapor within the chamber boo&me loss on the iteoond day of expos tire and still loss on the thlx'd. It is thought that tho tricresyl phosphate might have hydrolysed some what during the course of standing overnight, possibly from moisture absorbed from the air parsing through it during the day* In order to .-".aintain the oonoentratione ne uniform es possible, the material remaining in the towers was roplaood with ft fresh supply on the fourth- day of the experiment* Uad rufficlent material been on hand, this would have been done daily in order to attain a somewhat higher average oonoentration throughout an oxper- imont* This difficulty did not arise in the case of the Aroolor 121+8. NV 016482 715734 -9- C C N FI c ;j;7 /A ^ It was neoessary to employ other equipment for the volatilization of Pydraul 7-9 beoause of extensive foaming In the bubblers. Therefore, the Pydraul 7-9 was maintained at temperatures ranging from 109* to 112* C. in a glass well heated by niohrome wire (Figure 1). Air at the rate of 20 liters per minute, as measured by a rotameter, passed over the surface of the heated liquid and then through a manifold oooling bath before entering the ohamber. A volume of 190 ml. of fresh Pydraul 7-9 was added to the well daily, of whioh about 170 ml* remained at the end of the day. Generation of a Fog From a Heated Inoonel Surfaos. A rotameter for measuring the flow of air, a furnaoe for volatilizing and decomposing the liquid, a seml-potentiomoter (see below), a oooling manifold, and the ohamber for exposing the animals are shown in Figure 3 In these experiments the smaller rotameter, to the right in the photograph, was not employed, and all air (20 liters per minute) that entered the ohamber was passed through ths furnaoe. The oooling ooils were paoked in the bath Bhown and surrounded by orushed lot. The body of the furnaoe oonsisted of a 26-lnoh length of oold-drawn, seamless Inoonel tubing, having an outsids diameter of 1*3 inches and a wall-thickness of 0,0*19 Inoh. Pydraul 7-9, trioresyl phosphate or Aroolor 1 2 W NEV 016483 715735 - 10 C O N F I.D E N T IA L vma droppod upon the innor aurfeoe of th tube front a burette through an Inoonol tube welded to the uppor surfaoe of the heated tube near Its midpoint* This side tube load a length of 6 inches, on outside diameter of 0*675 inch, and a well-thlcknose of 0.091 inoh. Another piooo of iAoonol tubing 3 inches in longth end having an ouusido dla-'.eter of O.Jj3S inch and a wall-thickness of O.QlpV inch, was alo woldod to the upper portion of the furnace tube, to permit the insertion of a thermocouple In such a manner ae to noafluro the temperaturo at the upper Interior surface of the furnaoe* The distance from oenter to center of the two smaller tubes, whloh vrore parallel to one another and perpendicular to the furnace proper, was 1,75 Inches. i'he outside of the furnace was wrapped with 2 layers of molding mloa. The middle 10-inoh portion of the length of the furnaoe was olootrlcolly heated by means of 20 foot (9 wrapping to the inch) of A. W. u. Number 20 i'ophot wCtt wire, which haa a resistance of 0*659 duns per foot at 60 F. The wire was insulated with approxi mately 0.75 inoh of diatonaoeous earth and wrapped on the outaido with asbestos doth* The temporature of the wall was 'aecsured by an iron- cons tantan thermocouple attached to a semi-potentiometer calibrated against a student potentiometer. The thermo couple was in oontaot with the upper part of the fumeoe NEV 016484 715736 V-*jy*;**'*" --. /-*v '- .. .. ." ... -"-rjr* - 11 - -c o n f i d e n t ',A t about 1 inoh downstream from the point at whioh the droplets of material fell upon its lower interior eurfaoe. After the furnace had been brought to the desired temperature of either 1050* F. or 1250* P,, the hydraulio fluid was dropped from a burette at the rate of 0,067 to 0.245 ml* per minute (of. Table 4), 0,100 to 0,167 ml, per minute being the usual rate. Sampling and Analysis of Contaminated Air. When the undeoomposed materials were metered into the air in the ohomber, 4 samples of air eaoh day were passed from the ohamber, at the rate of 3*4- liters per minute, through 2 absorption towers (2.25" x 13" outside diameter), eaoh eontaining 100 ml, of an appropriate solvent. Redistilled ethyl aloohol was used in oolleoting trioresyl phosphate or Aroolor 1246, while hexane was used for Pydraul P-9. The sample passed through a coarse fritted glass diso (1 .13" in diameter) immersed in the solvent and plaoed parallel to the bottom of the tower and about 0,2" above it. The oontent of Pydraul F-9, Aroolor 1248 or trioresyl phosphate so collected was determined by measuring, by means of a Deokman speotrophotometer, the ability of the solution to transmit ultraviolet radiations of wave lengths 246 m^t, 246 tyu, and 264 n^<, respectively. NEV 016485 715737 - 12 - CONFIDENTIAL' A ourve relating the transmission at 2^6 iycto the conoentration of Pydraul P-9 in hexane over the range of 4.7 to J1 miorograms per ml. ia shown in Figure 5 A oomparison of the oorresponding curves for Aroolor (Figure 6) and trioresyl phosphate (Figure 7), are also shown. The alcohol used as solvent for Aroolor and tricresyl phosphate was freed from ultraviolet-absorbing impurities by the following procedural About 1750 ml, of 95 per oent ethyl aloohol were permitted to stand overnight with 10 g. of pellets of KOH and 7 6 aluminum foil' (0.003''). The mixture was then refluxed for half an hour and distilled at C the first 20 ml. being rejected. In experiments involving the exposure of animals to the produots formed on thermal decomposition of Pydrnul, Aroolor or trioresyl phosphate for no longer than J hours, from 3 to 6 samples of air were collected from the chamber for analysis, depending upon the length of the period of exposure* When exposure was repeated on the following day, a corresponding number of samples was oolleoted on the seoond day* Analyses were made for one or more of the following materials: phosgene, hydroohlorio aoid, free oresols and carbon monoxide. NEV 016486 715738 sC O N F i D E N T l A 13 When AroOlor 1214.6 was to be determined* the samples of air were collected in an ov&ouated -;lae bulb l.liS$ liter* in captoity. The Aroolor in the cample wee abeorbed in 100 ml. of redistilled ethyl alcohol, the latter beinfr. releored into tho bulb from an auxiliary bulb by opening an interposed stopoook. In the i.lrot few analyser, it wt> found necessary to froe the clcoholio eolutions from carbon root by filtration, but subsequently the need for po doin# was eliminated by the insertion of a plug of ;:.las wool botween the ohanber. and apparatus for collection. Suitable aliquot portion* of the alco holic solution were employed in the analyses. When phosgene was to be determined, the air withdrawn from the chamber waa passed at the rato of 6.(3 liters per minute first through a column 1.5M x 0.5n of 60 to -.'.0 moah zinc dust, next through a fritted rlacs dleo immersed In 100 ml. of a l|. per cent aqueous eolution of sodium hydroxide contained In a cylindrical tower 131' in height and 2.25M in outside diameter, arid lastly ttirouph an lipinter similarly immersed in a like volume of alkaline solution in a second tower of the same dimensions, "amplea of air to bo analysed for hydroehlorlo aoid were oolleoted similarly oxcopb for the omission of the column of sine dust (of. Jacobs, Analytical Chemistry of Industrial Foisons Hazards and Solvents, p, 305, 306, NEV 0164ft7 715739 V .CONFIDENTIAL Ik 19lj.l). /iter an aliquot portion (15 ml, or lees) oi` the relation hod been node aoid to phonolphthaloln with 10 per cent nitric acid (1 vol. of >,0, l*l|2 diluted to 7 volumes with water), and diluted to 5 * 0 :.<1, In a volunotric flnrk, 3 drops of 0.1 N silver nitrate wore added. rho trtmrnieoIon- of ll'.h.t of wove loiv.th 500 vyt tlii'ov:h t'.iJ turbid solution in a >0 rim* coll was ?>-eas- urod in a hoekman spectrophotonter in comparison with that through a similar cell containing a ohloricle-froo rolut ton prepared in a correspondlnp; *iennor. A ourve relating the transmission at 500 to the concentration .of sodium chloride over the ran.ro of 20 to 320 rnicrorrams .or P5 "l. la shown in .l.cure 0. On milllrram of sodium chloride is oqulvalont to O.i`%61 tr^ of phosgene or to 0.6230 v. of hydroohlorio acid. iho crosoln wore determined colorimotrlcelly after coupling, with diaeotlsod sulfanilio acid by methods for the meta ioomor described by Kosnot and "aman (Anal. Chom. 20951, 17l|.8), and for the x^ara isomer by 'ch-nldt (J. idol. Chem. 1791211. 19U9). 'ample of air for the determination of either trioroayl phoephat or free orooola (meta and para icomors) were passed at the rate of 6.8 liters per minute for 30 to 1*5 minutes through a fritted-claae dieo sorubber and lmpinger in eerie as in tho oaee of phosgene. In this instance, each tower NEV 016488 715740 ' - ' *- - -- 15* PONFIOENT/A L contained 100 ml# of a 1 por cent sodium hydroxide solu tion. In analysing for the free oresole, 1 r i l . of a 20 por oent bodium carbonate solution was added to an aliquot of 6 ml# or laaa contained in a 10 ml. 'lass-stoppered graduated cylinder. After the solution bad boon diluted to 8 rnl., 2 ml# of dlacotizod sulfnnlllc acid were added# The latter was prepared by adding 0,?5 ml# of a 5 per oont solution of sodium nitrate to 0.75 ml* of a solution of rulfanilio acid oontelnod in a 25 ml. volu.-.utric flask previously cooled in an loo-bath, i'ho sulfanilic acid solution was preparod by dissolving 1+.5 (; of tho acid in )+5~ml. of concentrated hydrochloric acid end diluting It to 500 rl. with viator. Aftor the partially diazotizod solution Vied beeii cooled for 5 minutes, an additional 3 '1. of codiuin nitrate solution were added and further cooled Cor 15 rainutos, then diluted to 25 rnl# i'he solu tion wan preparod dally and kept cold, Curves rolatlng the transmission of light of v/avo lengths !|?5 vyu. and 500 ryt to tho concentration of ota crocol over the rengo of 3 to lp9 taiorogriuns per 100 ml. are shown in Figure 9 In making the neoesaary measurements, the oomparlcon-oell contained a solution similarly preparod exoept for the omission of cresol. Corresponding curves for pere-arosol over the range of 10 to 120 niorograras per 100 ml. are shown in Figure 10. By fcho uae of the following NEV 016489 715741  O N F I D f i K j T fAfc 16 fliniulttmeoue equations, the amount of met* and para cresol could bo caloulated. For the datermination of mota oretol (r)t p-orosol 1|31 M-oreaol * apparent p-oreaol ik.2$ ny*) p-cresol + 1.13 M-creeol apparent p-creol (500 ryt) 3 18 M-oreeol difference lxi apparent p-ct'oool at the 2 wave length* (V) :i-crof>ol * dlfferonoe In apparent p-cresol 3~l8 ,or the dotornlnation of para creool (Y)t creed + 1 p-erool apparent K-oreaol (ij.25 2^) 4.13 i-crorol + 1___p-ori:ol * apparent M-orosol (500 tyt) 1.13 0.653 p-croEol a difference in apparent M-orosol at the 2 wave lengths (Y) p-orosol difference In apparent vi-orofol 0.653 fhe validity of thU .method for the almultaneoua do termination of the two inomora was dop'.onabrated by the following determlnatIona on sample containing both of them in known amount. Analysis of a aiviple prepared to contain 12.30Y of m-orosol and 9.962 Y of p-oresol yielded 13.S0 m d 9.95* respectively. A eooond eanple preparod to contain 2ij..60* of m-oreeol and 996Y of the para laoner yielded 25.90* and 10.7Y, respectively. The result* designated as free oresols in Table J4. reprosont the sum of the two iaomor. From 60 to 70 per m NEV 0164.90 715742 -* rrr-r .V " M ' f\rfm flan - 17 - CONFIDENTIAL cent or tho total amount of fro* oreaola present in the ohawber, a the result of decomposition at 1050 P., was found to ooneist of tha mota laomar* but whon tho tom* peraturo of tho furnace was 1250 F*, only tho mota Isomer was present* 3ineo a cample of trioreeyl phoapliato, analysed after hydrolysis, contained approximately per cent of the mota and 35 per cent of the para isomer, it appears likely tlusit the latter underwent decomposition to unidentified fragments at tho higher temporature* For the determination of undeeompoaed tricreayl phos phate proaent in tho chamber, a 5 nl# aliquot portion of the sample, oolleoted in the manner Indicated for tho vietermination of crecole, woe hydrolyzed by refluxing it for an hour with 10 nil. of lj.5 per oont aqueoua odium hydroxide) It wee thon acidified with concentrated hydro chloric acid and distilled until 300 ml* bad been oolleotod. The usual croBol method was thon applied to the distillate* Carbon monoxide was determined by a modification of the method of Bland (Jtust. J. .eper. Biol* cud Med* i>oi. l035# 19^0)* The samples were collected by passing air at the rate of lj.*7 liters per minute for 5 minutoe through the absorption train shown in Figure k by moans of a cast Rotary Bleat and luotion lump located benoath the table and not shown in the photograph* The first U-tube con tained glass beads and a 10 per oent aqueoua aolutlon of NEV 016491 715743 - x * confidential load acetate, while the seoond U-tube, which contained sulfuric acid (3,0, l,0Jp) and glase beads, was surmounted by a Ion?:, narrow tub* peeked fro top to bottom with cuoceseive layers of sllloa gel {i>iprognated with a oobalt salt to ludloate the pi*eeonoo of moisture), /ift" noslum perchlorate (Anhydrono), a odium hydrate afboatos absorbent (Accarite) and a eeoond layor of a. noslum porchlorafce, Air Insulin from the top of thin tubo passed through a tube containing: iodine pentoxide heatod to H i 0 C. Iodine liberated by the reaction I2$ * ^C0 50g I2 was collected in a bubblor containing 5 1 , of a 3 per cent aqueous solution of potassium Iodide, The rate of flow was lOfturod by an upright manometer as shown in rinuro Jp. The contonts of the bubbler wuro titrated with 0.001 II arsoniouo aoid, e.mployins starch os indicator, n^AeOy + i2 + :i2o -- * Hj Abo^ + a m One milliliter of 0,001 tf arsenloue aoid 3* 0.070025 mg, of CO. In calculating tho results, allowance was mad* for tho previously determined traoos of free iodine in the volume of the solution of potassium iodide present in the bubbler, bach of tho following concentrations of tho various contaminants of the air of the chamber, expressed as e NEV 016492 715744 19 - CONFIDENTIAL p.p.m. by volume in air at 61* P, and 7U5 mm. of meroury, is equivalent to the oonoentration of 1 ng. per liter. Compound Phosgene .ljas.tAJS*jpjtPA" i. 255.9 HO1 Triorosyl phosphate 693.3 6C .6 Crerol 233.8 Carbon rionoxide 902.7 Aroolor 121*8 131.7 hxperlmontal Foaulte -xpocure to vJndeoonpoeed Vapors. A group of animals, consisting: of a oat, 2 guinea pigs, 5 nice, 2 rabbits and 3 rats, survived when exposed to air laden with 3.8 mioro;.rams of lydraul F-9 per 11 tor for 7 hours on eaoh of 5 consooutlvo days (Table 1). All animals of a group sir.llarly constituted, oxoept for the omission of one rat, survived when exposed to air containing t*3.1 mioro- grams of Aroolor 121*8 for 7 hours on eaoh of 1* ooneeoutive days (Table 2). Lxoopt for one rabbit (P'798) that died from oauses unrelated to exposure i* days after the 8th exposure, thoae of a 3rd group survived when subjeoted to the inhalation of air bearing 61.5 mlorograns of trleresyl '\ phosphate for 7 hours on oaoh of 8 days over a period of NEV 016493 715745 ... /-> 20 * ^CONFIDENTIAL' 10 days (Table 3)* Charge In weight were usually email (Tables 1 - 3), and no eigne or Intoxication vara observed* Mlcrosoopio examination or tha lungs from animals exposed to Pydraul F-9 and killed 10 or 13 days after the 5th period of exposure, revealed the ooourrenoe of interstitial pneumonitis in the rat, mouse, guinea pig and rabbit. The eat exhibited moderate numbers of hie tiooytos, containing dark-brown granular pigment, within respiratory bronchioles and alveolar ducts* The remaining viscera were normal. Animals exposed to the vapor of Aroolor 12ij.O also had interstitial pneumonitis. The lungs of a eat whioh had been subjected to exposure to the vapor of Aroolor l?lj.8 were normal* There was no evidence of injury to the other vlsoera of the various spooies. The liver of a rabbit (f*907) the only one examined miorosooploally, exhibited severe fatty degeneration and foci of necrosis. Miorosoopio examination of tissues from a rabbit (F79&) that died If, days after the 8th exposure to tri- oreeyl phosphate dlaolosed the presenoe of a severe paraaitie infostation in tho liver* Another rabbit that was killed 3 days after the 8th period of oxposure had inourred severe parasltlo infestation of tho liver* A guinea pig had acute lobular pneunonla, unrelated to the experiment* In the lungs of a eat were considerable NtV 016494 715746 * - 21 - C O N F I D EM N T I A L numbers of histiooytes, oontaining dark-brown granules* The vleoera of the rate and loioe wore normal* Exposure to rroduotf Arising From fj&r*ul F-9* Aroolor iUkflj OP Trlorasyl rhosphato When In Contact with Inoonol at 1250 or 1050 F. The duration of exposure the ohanges In weight and the fate of individual animals that were subjected to the inhalation of the produo to. arising from the contact of Tydraul F-9 with Inoonel at 12i>0 and 1050 F* are proton ted in Tablet $ and 6 rospeotivoly* Corresponding data from animals exposed to the fogs from Aroolor'12IJ.8 or trioresyl phosphate are given rocpectively, in fables 7 and 8 and in Tables 9 and 10 The data relevant to mortality among all groups have boon summarised in Table 11 Mortality Careful oxanination of the data demon strate oertaln oneral foots* It is apparent, in terms of ovor-all mortality, that euinoa pigs and nioe were u more nusooptible to each of the three materials than were rats and rabbits (Table 12)* The same orltorlon was employed for comparison of the fogs that were formod at 12i>0 and 1050 K* The fog generated from Aroolor 12l|.8 at 1250 F. wee more toxic than that formed at 10$0 (Table 13)* In the eaee of ^ Tydraul F-9, the toxieity of the fog formed at the higher temperature appeared to be slightly greater than that NEV 016495 715747 22 L?;N Pl DGNTf A l generated at the lower* The temperature of dooomposltion vac of little importance In determining the toxicity of the fog formed from trloroeyl phosphate, alnoe that vhloh resuited from the higher temperature was apparently slightly less toxlo than that formed at the lower temper ature* A comparison of t-ho three materials under corresponding conditions indioatoe that the fogs formed from Aroolor 121*8 wore more toxlo for guinea pigs rabbits and nice, but not for rats, than vero those formed from Pydraul :-9 or trloresyl phosphate (Table 12)* Aroolor 12l*8 ie also the most toxlo of the three material if the shortest period of exposure that Is lethal Is taken as the or1 mo rion. Regardless of the tomporature of decomposition, all animals survived when subjooted to the fog formed by fydraul i'-9 or trloresyl phosphate for 1> or 20 minutes, but exposure of like duration to the fog oroatod by the thermal decomposition of Aroolor 121*8 often resulted fatally. In ozder to ascertain the effeot of different retea of dolivery of the liquid into the Inoonel tube while the rate of flow of air through the tube wae kept con stant, Aroolor 121*8 and trloresyl phosphate were delivered at two different rates (Tables l* and 11) The mortality associated with reduction of the rets of delivery was relatively low in many Instances NfcV 016496 715748 GONFIOCNTIA 23 ftlrtns and Pathology of Intoxication. Although in those experiments th fogs filled the chamber to euoh an extent that it was impossible to obsorv the animals throughout tlie period of exposure, a few signs of intox ication could bo observed* 'he fogs wore innodiately irritating to tho mucous nonbranes of tho animals, as ovidonood by their closure of tho eyes, twitching of m e nose, crying, couching and sneesing. Labored roapiration and irritation of tho superficial tissues of tho oyes appeared lator. Dyspnea was observed after 20 minutes of exposure to Aroolor 12IfC, but this did not appear until tho animals had boon exposed to tho other materials for almost an hour. Lhenovor oats wore exposed exooseive salivation was ovident, end frequently long strands of thick nuous drooled from tho mouth, .'lack coot covered tho hair of animals exposed to fogs generated at tho hIf her temperature (12>0F.). examination of animals that died.during or shortly after exposure to the fogs of fydraul P-9, Aroclor 12lj8 or trlerenyl phosphate revealed extensive pulmonary con gestion and, in occasional animals, fool of pulmonary hemorrhage .Many also had foci of black pigment that appeared`to bo oorbon distributed throughout the lung tissue. Miorosoopleally, the lesions oonslsted of narked hyperemia of the lung, common to all exposed animals NEV 016497 715749 21). - examined, and In many there waa intra-alveolar hemorrhage and edema. The carbon pigment waa distributed chiefly within the bronchi and in some instance, completely odbluded thoir lumen*. Pulmonary emphysema was presont frequently. In 5> guinea pigs and 2 rabbits that died during; exposure there was, in addition, an aoute purulent bronchitis, apparently a reaotion to bronohial irritation. Lathslly poisoned animals exhibited parenchymatous degen eration of the liver, kidney and brain with regularity, in addition to thd, ptQjsonory lesions. The viscera of animals . that died 7 *%v, to '10! day after brief periodo of exposure revealed pulmonary lesions similar to those already described except that there *as more phagocytosis '-X ' v !-y * of oarbon partloles. Aniiria'ii.that survived were killed after intervals varying from 1 Veek to 2 months. There 'were no signlfloan^ lesions of visoera other than lung. Two oats of the 5 exposed to the fog of Pydraul i'-9 wore examined miorosoopioally. The visoera of Oat A38l were normal, while the lungs of Cat A37lf were the site of pro fuse intraluminal bronchial deposits of blaok granular material and interstitial pneumonitis. The lung of Cat A371, whloh had boon cubJoted to Z periods of exposure to the fog of trlcreayl phosphate, was characterised by a/'*, similar pulmonary changes. Guinea pig A862 days after 2 periods of exposure to triores NV 01A4QA 715750 m i-<yr>y fyyqwyi WWW. - 5 vaporized at 1250* F., was found to have numerous histiooytes loaded with oarbon pigment In the lungs, as well as a severs Interstitial pneumonitis. Other speoies of animals had a similar interstitial pneumonitis. However animals that had suffered the shortest periods of exposure, and animals killed 2# days or longer after exposure, exhibited normal visoera. The Concentrations of Identified Decomposition Prod ucts Formed at the Higher and Lower Temperatures. Although only a part of the materials metered into the Inoonel tube oould be aooounted for in the air in the ohamber, some very tentative statements oonoeming the composition of the air may be made. Although large amounts of soot were formed, no attempts were made to measure the amount of free oarbon or of carbon dioxide. More unidentified materials were present when the decomposition was effeoted at the higher temperature, irrespective of the nature of the liquid dropped into the tube. In the case of Pydraul F-9, less hydroohlorlo aoid and more free oresols were present when the temperature of oontaot was 1050 F. than when it was 1250 F. (Table, h) When the temperature of oontaot with Pydraul v/as 1050* F., the oonoentratlons of hydroohlorlo aoid and phosgene, measured together in terms of hydroohlorlo aoid, ranged from 0.35 to 0.67 p.p.m., but when the temperature NV 016499 715751 was 1250 F the corresponding values ranged from 11 to 22 p.p.ra. Free oresols were more abundant at the lower temperature, being present to the extent of 11 or 12 p,p.m., whereas only 0,09 to 0,15 p.p.m. were found v;hen the temperature was 1250* F. In experiments with Aroolor 1243, nuoh more undeoomposed material was found present when the temperature of contaot was 1050 F. than when it was 1250 P., 121 to 266 p,p,m. being found in the former oases and only 10 to 16 p.p.ra, in the latter. Only rarely was more than one p.p.ra. of phosgene found regardless of the temperature, the values reported as hydroohlorio acid and phosgene representing predominantly the former. Less hydroohlorio acid was present at the lower temperature, there being 11 to 17 p.p.ra. in this ease and 34 to 102 p.p.ra. when the temperature was 1250 F. The values reported for oarbon monoxide are probably somewhat low beoause the rotameter employed for the oolleotion of the samples had not been calibrated for use under the conditions of these experi ments. Nevertheless, it seems apparent that when the Inconel was kept at the higher temperature the amounts of oarbon monoxide found tended to inorease as the duration of the experiment inoreaaed} muoh less variation was encountered when the Inconel was kept at 1050* F. NEV 016500  27 O ' A/ !A l In the oase of trioresyl phosphate, more of the undeoompoeed material and more free oresols were present in the ohamber when the temperature of the Inoonel was lower The values reported (Table 10 for trioresyl phos phate, as determined by hydrolysis and the oolorimetrio determination of the resultant oresols, may be somewhat low, oinoe results several fold higher oould be obtained by measuring the ultraviolet absorption at (& iy* of nlooholio solutions of spot samples oolleoted in an eveouated balloon. Determinations by this weans may, however, have given high results, since filtration failed to remove a slight smoky turbidity. NEV 016501 715753 C o Np, 20 tf* L Pisouse loft It Is unlikely that any of the fatalitioe in the# experiments oould bo attributed solely to the presence of phosgene, since, according to i'lury and 2ernik (Harmful das, Berlin, 1931 P* 222), mice must bo xposod for several hours to the concentration of 1.25 p.p.u*.. if any are to be killod, while only 70 per oent of a group of lnico died when exposed for 20 minutes to air bearing 20 p,pra. On numerous occasions the nloo in our experiments dlod when exposed for but one hour to an atmosphere that ccntalnod lose than 1 p,p.m* of phosgene, Flury and V.ornik state also that rabbits survived following oxpooure for 0 minutes to the concentration of 5>0 pp.m, but that 75 por cent of another group died vfnen expotod lor twice that period. Box and Cullumbine (Br. J, Pharmacol and Ghom, i30# 19ip7) report tiiat 8 of 12 rats died after being exposed for 10 minutes to 0,23 mg of phosgene per liter of air (7*5 p*p.m), although the concentration of 0080 .mg* per liter (20*5 ppm.) was not lethal for rate when inhaled for 10 'minute* In the oonoentratlon of 13 to 20 tog per 1, ( H 7I4. to 1805 ppm), carbon monoxide oeused no significant signs of intoxication In mice exposed for 6 hours by Cherno and L ib erm an (F e m a k o l. 1 T o k s ik o l. (Wo. 3 ) t 2 2 , NEV 016302 715754 19l*7l 0. A. kS,l2679# 191*8), although 20 to 1*0 per oont of a group of mice died aa a result of a like poriod of exposure to tho concentration of 6 mg* of oarbon monoxide par 1 (1*16 p.p.m.). Since tha mice in our experiments died under conditions whioh were rauoh laaa severe with respeot to oarbon nonoxIda, it Is avidant that this oon- pound by Itself waa not raaponalbla for their death. Comparable Information with raspeot to other speoiaa of animale la not readily available, although Moes, Jaokson and. Saibarlioh (Arch. Ind. Hyg. and Toxlool. i62, 1951) 'nave reported that the concentration of 5*000 p.p.m. waa required to kill a rat in 30 minutes. Rabbit* and guinea pige survived when subjooted to the Inhalation of the vapors of hydrogen chloride in tha approximate concentration of 250, 700 and 1,000 p.p.m*, over the respective periods of 7* 2 and 0.08 hours (Troon et al.. Arch. Ind. Kyg. and Toxiool. <716, 1950). in these same exprimenta, some of the guinea pige and rab bits died when expocod for 0 to .0 hours to concentra tions of hydroohlorlo cold ranging from 1,000 to *00 p.p.m. Again, hydroohlorlo aoid doos not seem to be the sole agent responsible for the deaths encountered In the present experiments. nev 016503 Data pertaining to the toxloity of oresola in air are not available) however, Deichmann, Kitamlller and 715755 Witherup (An* Clin, Path. 4,173# 191*4) Found that only oftor 20 period of expouro, eaoh of 7 hour, to the vapor of phenol in the air in tha conoentratlon of 0,1 to 0.2 mg, per 1 , did any respiratory diatro or fatallti dorolop among a group of 12 guinea pig Rabbit and rat survived much longer, Sinoe it la likely that the vapore of meta and peraoroeol are comparable in toxio* ity, it doee not appear that the death reported herein ore attributable to croaol* NEV 016504 715756 31 4* .0 > * /s1 . gupplpntrr a.tgttatnfc Th Appll^tlon of Wrftul. r-9 APPQloy m g and Yrloresyl Phosphate Vpoa the Skin of Rabbit* Kxperlwontal Hethods In aooordance with the teohnique of Drelse Woodard and Calvary IJ fharm* xper. Therep. 1377 19UH), one of a series of graded doeages of each of the undiluted material* Pydraul F-9, Aroolor lSlj.0 and trioresyl phosphate, was maintained for 2k hour* in oontaot with the iptact skin of one of a group of rab bits in this method, the hair was olipped from an area of akin 6 to 7 inohe* wide and completely enoiroling the trunk of eaoh rabbit In other group*, the outermoat layer of skin was abraded by scratching thin furrow* 75 071 in length and a few millimeter* apart with an hypodermlo needle In eithor case, the entire trunk wa* covered with a sleeve of dental rubber dam whioh fitted tightly at the oophalad and oaudad edges of the dipped area and loosely over tho intervening area During the 211-hour period of oontaot the rabbit was kopt in stook* At the end of the 21} hours, the material was removed from the skin of the animals by washing with water and a sulfonated oil, "pH6" In addition to the three materials mentioned previ- NEV ously, a product designed to fulfill the sane purpose, 715757 *,V>yTri " /..fv - 32 C A'p ^0 Gargoyle DTK, wee investigated. Gargoyle PIS (Socony) 1 said to bo a petroleum bat hydraulic fluid containing minute amount of a ruat inhibitor and an oxidation Inhibitor* Gargoyle DTE hat the epeoiflo gravity (API) of 31 1# a maximum pour point of 20 F.# a minimum flash point of 395 F., a fire point of 14-97 F* It visooslty at 100 P*, 130 F. and 210 F. 1 225* 116 and 40.5 S*3*P., respectively* Experimental neaults* The pertinent data of the oxperlmont relating to Pydraul P-9# Aroolor 121)8, trioroayl phosphate and Gargoyle PTE are given in Tables l!j, 15, 16 and 17# respectively* Table 18 summarises the essential information in the preceding tables* Two rabbits died following ths application of 9*4 ml* of Pydraul F-9 por kg* of body weight upon the lntaot skin of eaoh, whereas only 1 of 3 rabbits died when tho dosage vaa 6*0 ml. per kg* Three rabbits sur vived when e&oh was subjootod bo the application of 3*6 ml* per kg* Tho dosage of 6*0 ml per kg* was lethal to 3 rabbits when applied upon their abraded skin (Table 14). Two rabbits died following the application of 6*0 ml* of Aroolor 1248 per kg* of body weight upon abraded akin of eaoh, wheroas only 1 of 3 rabbits died when the NEV 016506 715758 dosQge was 9.4 or 9*0 ml. per The dosage of 9*4 wi per kg. was tolerated by 3 rabbits when applied upon their intaot skin (Table 15). The application of trioresyl phosphate upon the Intaot skin resulted in the death of a rabbit given 9.4 ml. per kg. and in that of another given 6,0 ml, per kg. When the dose was reduodd to 2.5 ml. per kg., 1 of 3 rabbits died, whereas 3 rabbits, eaoh given the dosage of 1.6 ml. per kg., survived (Table 16). Five rabbits with Intaot skin and 3 rabbits with abraded skin survived following the applioation of 9.4 ml. of Gargoyle DTK per kg. upon their skin (Table 17). In no instanoe was the Intaot skin of the rabbits injured by the applioation of these materials. Nothing remarkable ooourred when Gargoyle DTE was applied upon the abraded skin; hov/ever, the abraded skin beoamo very slightly erythematous after Pydraul F-9 or Aroolor 1246 had been applied. From the Kettering Laboratory in the Department of. Preventive Medioine and Industrial Health, College of Mediolne, University of Cincinnati, Cincinnati, Ohio. Experimental Work and Report by: Joseph F. Treon, Ph. D. Frank P. Cleveland, M. D. Frederio E. Shaffer, M. S. John Cappel, B. S, Thomas Gahogan William Wagner Approved ___________________________ Date Maroh 11. 1953 Robert A. Kehoe, M. D, Direotor N6V 016507 715759 ''V O. >/ The Mortality Length of Survival, and Chjftnr.ee In Weight of Animals Exposed to the Vapor of Pydraul F-9 In Air (Concentration of fydr&ul F-9* 0.00377 ) (duration of ibcpoeurej 5 x 7.0 lours) Identification Number Change In Weight Expressed as Percentage of Initial Weight Initial During After Weight Period of Period of kg. exposure Exposure Fate Longth of survival After ocpoBure .'ofore the Animals /ere Killed Days Gat A359 Guinea I'ii" A7^0 2.$0lf 0.9 09 '.:\;ir.oa Pis A7tj.l 0.961 2 Mice 3 -ice ) Av. ) 0.0198 ) Rabbit iC2l| 2.038 Rabbit f02$ 1.963 Rat DJ53? o.2l*0 Rat Dtk0 0.209 Rat r>5l*l 0.252 1 Ij. -3.1 -5.il -0.9 +1.2 +3.3 -1.6 M + 1.3 -- 0.5 _ +11.0 m +15.7 + 3.8 + 1.2 Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived 10 10 13 10 13 10 10 10 13 13 NEV 016506 715760 '** i-.v - - '* **+*/-T** *'jr"r**rr:'rr*'*rm ; . C 0, MUJL Th# MartiHSra aurrira. m l Ohw>t in weight gL.^i^-njfeP.aftgA.fcgJt^t.-VMiMr.,.jyes&toK i M (Concentration of Aroolor 12J$8i 0.0i$31 mg./l.) (Duration of Exposure 1$ x 7.0 Hour) Tdontlfloafclon Number Change, In Weight Expressed aa Percentage of Initial Weight Initial During After Weight Period of Period of kg. Exposure Exposure Fate Length < Survival After Expoeur Defora tt Anlraali Were Kill Daye Cat A378 Guinea Fig A757 IJ..300 0.9C8 Guinea Fig A7$Q 0.939 >' Mioe Rabbit F906 Rabbit F907 Rat L203 Hat E28l$ Av. 0.0178 2.01)1$ 2.U$1$ 0.179 0.170 - 2.6 - 1+.6 1|.2 +12.1$ - 6 .1$ + 1.7 +11.7 +14.7 - 3.0 0 - 2.3 + 6.7 +13.1 a +11.2 {Survived survived Survived 2 Survived 3 Survived Survived Survived Survived Survived 6 6 6 6 7 6 7 6 7 NEV 016509 715761 * '/a* -w*v<,'?tnrM'ncr%TJ''W f V I.J ^ !' IN Iflp 1>.. TH rV^f^rr r / * * - aau Tn Mortality. Length of Survival. and Changes in Weight of AniwtXi R ^ Q M 4 to tht_VftBor__ef_ TricjttejiphQepbate m Air (Concentration or Trloresyl Phosphate 0.06X5 mg./l., 4*2 p.p.m.) (Duration of .Exposure 8 x 7*0 Hours) 4 Change in Weight Expressed as Percentage of Initial Weight Initial During After Identification Weight Period of Period of Number kg. Exposure Exposure Fate Length of Survival After Exposure l<ofore the Animals Were Killed (K) or Died (D) Days Oat A353 3*226 Guinea Pig A733 O.809 Guinea Fig A734 0.811 5 Mice At . 0.0X64 Rabbit 1797 1*779 Rabbit *798 1.841 Hat D5>36 Rat D537 0.130 0.140 Rat D53 0.129 + 5*6 1.6 - 0.5 +13*4 4*6 + 6.5 +14*6 7.1 +28.7 0 + 4.8 mm 0 m 6.2 +25.7 m Survived 3 K Survived 3 K Survived 10 K 2 Survived 3 K 3 Survived . 10 K Survived Survived 3K 4 0 (U Survived 3 K Survived 10 K survived 10 K (X) Death due to parasitic infestation of liver unrelated to exposure NEV 016510 715762 Table 4 C C,. Concentrations of Various Decomposition Products In Air of Chamber In the Sereral Experiments Ar; Material is Contact With Inconel Tariation In Temperature of Inconel F. Experiment Number Material Added ml./min. Expressed as mg./l . on Upper Line and p.i>.m. on Lower Line Aroclor Phosgene HC1 (uncor rected for Phosgene Tricresyl Free Phosphate Cresols kt Carbon Monoxide Pydraul 1240 - 1256 1 0.143 - 0.0326 22.7 - O.OOO65 0.15 Pydraul 1245 - 1260 2 0.133 - Pydraul 1240 - 1250 3 0.167 - - 0.0160 - O.OOO52 12.5 ] 0.12 0.0164 11.4 - 0.00036 0.09 i i Pydraul 1245 4 0.245 - Pydraul 2 1245 m 5 0.220 - Pydraul e 1040 1055 6 0.132 mm H* O' SJ> - 0.0155 10.7 -- - 0.0005 0.35 -- mm -- - 0.0459 10.7 -! 1 Material In Contaot 1th Inoonel Variation In Temperature of Inoonel F. Experiment Number < C /V h* T a b le 4 (Page 2) - -[1A- -- & Expresse'd as mg./I on Upper Line and p.p .m. on Lo1ear Line RC1 Material Added (unoorrected for Tricresyl Free Carbon ! ml./min. Aroclor Phosgene Phosgene) Phosphate Cresols Monoxide .i Pydraul 1047 - 1052 7 0.125 Pydraul 1049 6 0.113 - 0.0012 0.83 - I 0.0465 `* 11.3 # f4 i O.OOO96 0.67 - 0.0467 11.4 - ' : ' " oj Pydraul 1049 Pydraul Aroclor 1047 1247 - 1256 9 10 1 0.146 - - - 0.100 0.113 mm 0.124 16.3 - 0.00203 O .52 0.121 33.9 - 0.0513 12.0 - 1\J$ -1 - -J - - 2106..274 i 1 Aroolor 1247 - 1252 2 0.144 0.111 0.0166 0.147 - - 0.0366.9 14.6 4.6 101.9 33-0 i Aroclor < 1249 - 1252 1o-- 3 C.125 0.077g 0.0012 10.2 0.31 K> - - o.ooifcj y -- .. -- ---S4I 715764 racle ii U^a^ie 3) Material in Contact With Inconel Variation in Tenterature or0Ipn.conel experiment Sunber corial Added ral./min. expressed as rs^./l. on Opper Line and p.p.m. on Lower Liai Aroclor phosgene UC1 (uncorrected 1er Phosgene) Tricresjl Fhoephate Free Cresol* Carbon! aonotl Aroclor 1252 4 0.130 0.102 - 13*4 -- 0.00071 0-7 Aroclor 1249 5 0.069 0.0573 0.0109 0.0143 - 7.5 2.6 9.9 - 0.025^ 22.7 -- i Aroclor 1252 6 0.067 0.0367 0.0015 - 5.1 0.4 - Aroclor 1049 - 1053 8 0.121 2.17 1 0.00035 0.0224 - 285.8. 0.0\9 ,^* , 15.5 Aroclor 1049 - 1053 7 0.122 1.80 237.1 00..1080071 0.0154 10.7 - - 0.0386^ 34-8 I - ...4 - 0.0260! 23-5 -ait * - 0.0308;' 27-8 Iroclor 1049 - 1051 <zm 9 .0.121 1.37 O.0C037 0.0253 - 180.4 0.C9- 17.5 - 0.0146! *13-2 4! iroclor 0 1049 O' 10 0.120 1.24 mm 163.3 0.0190 13.2 mm i 0 .0 220 ] 19-9 1 ; ' >J 4 715765 fable k (i-se Ij.) NfcV 0i651< < ? ir d O*O' 09 OM r OrvOi d "TT"" r-- -- - 1 t raOtH.] # OH 1 !.. a0 n a a <D*Ul. .* *3 I 1 i s 8h OH 3CoOMvOm OO s 8* oo o NO 8^0 OH ft >* i oU0<Cu ,i 1 1 fiHA 8to CO ooo O O OsO ft $ ij &o t4> '-V t0 uo ~o ic.-: Qr t OQ f S oi..'; a o.9 -'S*- I HCHO*-O d roOO-tC--f# ft 11 11 < T \) mv c0otfa> 1 8Oir-ti OO 1 t1 11 'o?W0<) t 5 HtoO CO r\-f>4 0> oo 3rHrO-f CHAVA H CM 1 1 f ft ft adH iH *H *q3 o t0T*r.i*a\ <rM4 o $o o 3O o i O o 3 9 o * <ri CCCMAA OHCM OO *> s1l l a <r-At $ CM H -d- v0 r iU SaSI-* 5 fhH H 3l * !!?] H* ! U\ s V<MA S CVCOAHM VHC<AMM C\ 1 c"f VOoHA f f VA f ft H * 1 1 ! n I I il il l il il 715766 rable 5) '` n + L i 4 Material ln Contact With Inconel Trlcresyl Phosphate >urx&i>ioji ln Temperatur of Inconel o p. 1214-5 fcxperirient Hunber 8 iiai-erlal Addod isl./min. 0.071 ixprecscd as r.:_./l. oa Upper Line and p.p.n. on Lover Lin; Aroclor e rhoscene - aci (uncorrected Tor Phos^ene) - Trlcresyl Phosphat -. Free Cresols 0.0011 0.3 11i Cartoon * MODCMCld^ f4 1 Trlcresyl 1258 Phosphat Trlcresyl 10^5 - 1057 Phosphat Trlcresyl 1014-9 - 1051 Phosphat 7 14 13 0.072 0.130 0.125 - - mm - - - mm *1 ' * / ] mm - 0.011 2.6 - - 0.098 23 -1J 07* 3 J? Trlcresyl 1025 Phosphat Trlcreeyl 1053 Phosphat Cricrasyl Phosphat Cricresyl Phosphat 1051 1014.9 - 1051 frloreayl 1051 Phosphat 3 10 n 9 12 0.120 0.175 0.195 0.068 0.0C2 s - mm - m o.55o 0.0565 0.025 37.8 13 22.6 j i mm mm - - 1 - -- f mm - 0.35 81 1 J i i mm u1 NtV 016515 4 715767 c c **- Mi The Mortality, Lon. t-h of urvlycl, and ahr-nr.os In `..'el,'ht ci` ;u yUT-Ig -CpQgod to Aerosols For-oi by Jrop,.lri^ fydraul r-9 Into an Incur a l Tube UeavoO to f anperature of 1250 F . (&77 o.) fixper- Duration of laent xposure Identification #urber iiotirs Kunber Change in Weight Expressed as Percental.: of Initial *.ei.ht Ini i/ii0L During Weight Period of k/i* Exposure Aftor Period of uJLfcrOi'UI*0 A> . Fate as Length of Survival looted at of Animals That Lind of . Died (D) or 'era bast Killed (K) Period of After Laat id.oosure Period of Exposure 1 6.0 + 6.25 6.0 Cat A37U Guinea Tic A759 : <0.692^05 1i -lo.O - lo2 6.0 6.25 Guinea ?i A?60 1.020 ! -11.1 6.25 3 Mice 6.0 + 6.25 2 Mice 6.0 + 6.25 Rabbit F932 ) ) Av. J.Goil 2.620 - 6.6 6.0 * 6.25 1 Rabbit F933 6.0 Rat 301 2.550 0.20? - fc.2 - 1.0 6.0 Hat E302 0.208 - l.if +11.2 - + 1.8 +10.0 + k.5 - -- .Survived Jied ~urvived Jl6U Survived urvived Died -led 7 days K - 7 days K 7 days K 7 days K - 2 ' 6.75 6.75 6.75 6,75 6.75 6.75 6.75 6.75 6.75 Cat A379 Guinea Pig A761 Guinea Pig A762 MOUB0 A2+35 3 Mice Rabbit P934 Rabbit F935 Rat 303 Rat 306. 3.170 0.6-50 0.1*65 0.025 Av.0.020 2.267 2.270 0.160 0.205 - 9.9 - 1.8 0 - 6.0 0 0 - 2.2 - *..9 + 3.3 -- mm +16.0 +11.2 + O.it Survived Died Died Gurvivod died 0..rvived 0urvived yied Died 2 months K a 15 day K - d days K 6 days S - 715768 NtV 016516 Exper Duration of iment Exposure Ideatificetion Furaber Hours Xuhber rabio 5 (irar,.e 2) r Change in Weight Expressed as Percentage of Initial Weight Initial Weight kg. During Period of Exposure After Period of Exposur Fate aa rioted at i^Dct or Last reyiod of Exposure C l 'irDZ N T A t Length of Survival of Aniiaala TSxat , Died (D) er U m Killed (K) After Laat Period of Exposure' 3 3.1 Cat A370 3.060 - 7.5 3.1 Guinea Fir; A763 0.465 - 2.7 3.1 Guinea Fig A764 0.480 - 7.1 3.1 3 Mice Av.0.015 -. 3.1 2 Mice a t .o .0175 - 3.1 Rabbit F93& 2.170 C 3.1 Rabbit P937 2.470 - 6.6 3.1 Rat E305 3.1 Rat E306 0.200 0.200 - 3.5 - 1.0 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 Cat A3S1 Guinea fi*.- AT 6 $ Guinea Fi.v. A?66 4 Mice 1 !*ouse Rabbit *93# I^abbit F939 Rat i-307 Rat S306 2.325 O.i&O 0 .4 1 0 Av.0.017 0 .0 1 5 1.996 2.030 0.205 0.186 - 1.9 - 1.6 0 0 0 0 - 6.8 -10.6 5 0.25 Guinea Pig A767 C.450 - 2.2 0.25 Guinea Fig A T6 8 0 .4 6 0 - 4.6 0.25 5 Mice A r .0.0156 0 0.25 0.25 Rabbit F940 Rabbit F941 2.115 2.050 - 4*4 0 0.25 Rat S309 0.187 -14.4 0.25 Rat E310 0.186 - 6 .5 +10 e0 - + 9.2 - + -- - + 4.6 .- + 5.9 -- +53.0 27.1 +27.6 +25.4 +32.4 + 6.4 +1 0 .7 + 8.3 +37.9 +24.5 +36.9 +30.3 Survived Died Survived Died survived Survived Survived ; Died Died survived Died Survived Died Survived 'arrived Survived ;urvived :.urvived ;urvived : urvived :Urvived Survived Survived Survived survived 7 y 'K 7 day K f -- 15 days 7 daya 2 day X X 0 ' - 9 days K -. 15 daya X - 14 days X 15 day K 15 days K 14 daya K 15 daya K 24 days K 24 days *K 26 days K 28 day K 28 days K 26 days K 26 daya K NEV 0165 17 > ; 715769 "able 6 P C N "IvSNilA L The Mortality. Lea-:tb cf 'igylval end Cbojn. es in .'el; ht of An.usalg exposed to erogols rorsed by /ro^liy p.ydraul F-y Into b~ Inconel lube Heated to a .i'emperatur of 1050 F ($66 C.) Exper Duration of iment Exposure Identification Rusher Hour lumber Initial Weight kg. Cliange in height Expreeaod aa Porcentai.e of Initial .eight During ioriod of jjcposure Ai ter Period of .exposure Fate aa I-oted at jumIUL V/A Last toriod of Exposure Length of Survival of Animals That Died (D) or Vera Killed U ) After Leet Period of Exposure 6 6.5 6.8 6.5 Cat A376 Guinea fig A?69 30..405805 6.5 Guinea Pis A770 0.408 6.5 6.8 1 Mouse ) 6.5 6.8 1 Mouse ) AT.0.0184: 6.5 6.8 3 Mice ) 6.5 + 6.8 Rabbit F942 6.5 6.8 Rabbit F943 2.190 2.140 6.5 *6.8 Rat 311 C.235 6.5 6.8 Rat 312 0.208 7 6.8 Guinea Pig A771 0.490 6.8 Guinea Pig A772 0.465 6.8 3 Mice ) 6.8 1 Mouse ) Av.0.017 6.8 1 Mouse ) 6.8 Rabbit F9kk 2.055 6.6 Rabbit Y9h$ 2.140 6.8 Rabbit F946 2.410 6.8 Rat 326 0.230 6.8 Rat 327 0.260 - 7.3 - 5.3 - 7.1 e* -14.4 -20 Ji - 4^8 ' - 5.9 - 1.3 _ - 05.1 _ -13.0 - 8.6 - I;*e2 - ~22e6 +33*2 - -- + 6.4 -+2123..51 24.6 -28.6 Survived Survived Died Died Survived survived Survived Survived Survived Survived Died Died Died Survived Survived Survived Survived Survived Survived Survived 3 aontha C 16 hours D 3 day 7 day 17 day 4 day 3 day 24 day D X D D D K m m. . 3 day 5 day* 24 day 28 day 6 days D *D X X 9 29 days X , 1 MBit> nFv n i 65 lb 715770 ^ D - S T t A - L sole 6 (la. e 2) 2si" | LirSI ^s fc si ea is ni 'v; ^ <0 xi ri O H k OQ O M 3 *<t*> *p9 P ii 0 10 p c$ ai p ri 4 ,rs a a -J o h r*< o 0 o^ :>* rH r i C vS t i vi S-*o3^o P O tt H H O % 5$S8SM ;l rii s a3 u a 'M w M |k4MW w o m w w | mw ' l i I l `f i l i t i * vDvn HC3CO 0 ><H 4 0 W H N N n e g h i hi ri CMOI (\JC\J _1 I_1151S1S1S1S1S1S 151S 1 S151S1S1 S1 S1 s S s & m & t5i5 l'.} .0 W a c 'Si vi V3 :: o i*> c.1 j/) * f ? ? ? ? ?1 1 1 1 O CMO *r|-iA cg * O lii <A | i t i iia v a ^acoco H VN-CO V 0 O 4 Cvj CJ fA cA cO H H - r f N HO ^1 > 0 r^r*\ I l i o f ^ r t N ( \ j c g <\4 rH A Il I t i l i O M rt(*\ 1 1 t ~ i*\ 1O O f^O O li? MM iiMII H rS .r fc o COCO O'O evi (\i ( v in i ?ii??iii ssssssss e l <-/ :/> :/) Ci (0 y> o t - O t uiv nt < iA \lA iH H v.0 1 i r \ fA .5%p^. cu H c'Vsl-Cavi'J + +++ + M cvj t*. 1A O ,1f --O O O O O P - O 1 rH 63 P A !> u) fy) 3 ?ri H 4 ri 0 sPrHi|* si 1M <H Sg 4jji ^QiCQ Q VA rO O O O O O R S 2 3 S R 8 RO ca cvj ?,>u \ - r j co CO O O (\i O C\J Csj i t i l i 1 f O 0> r i co CVj Oj # OO O OJ Od r-t O C) O O O H N H O O > -< << CO-^HA IO v j u n -a i a H ri 0>C ^O 6 6 6A* Ai 1^4 Pm CO 0 * 00 Cvi CSJ 6 6 4 ) i ) 4 l CACA O O O lP H n H H ^ ^ f l n * H Q O rO (D I > 0O rr>H H 203 A ^ O <tr**ta5+t4a ROuft O0 ' O i i , k 6,O H II8SSSS3S uo olnu^lSik lik OisChO Qi CA i t it I o o o o o o o o orr\r\c*\(+\tr\rr\er\r<\rr\r*\ CNO ( A \A V \W O lr\X A Q W \ ajvO O O O N N M O O O* N N H O O fN<-r<i<t:> wm tri Ai Ai vOO&\^QC&>h3QH(okv<<\|<*\ C djjAA 5 o * H M . r i r 4 M Umiliai OOOOOOOO 1! CO t5 ll ^ * S NV 016519 1 715771 The Mortality, Lcn; t':x of Survival end Chan. es in Weight of Anlnals Exposed to Aerosols Formed by Drop;.!nr% Aroclor 124$ Into an Inconel Tube Heated to a ieyrpera fcnre of 1250 r'. (677 C.) 4* i Exper iment ifunber 1 Duration of .exposure Hours 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Xden tif lection 1maker Initial Weight kg. Guinea Pig A779 i 0.415 Guinea Pig A7C0 -.475 5 Mice /v.0.0154 Rabbit F962 Rabbit ></63 2.105 2.205 Rabbit P96ii 1.960 Rat 334 0.220 Rat 335 0.250 Guinea Pi A7o2 Guinea Pig A703 5 Mice Rabbit FV'BC Rabbit F9&1 Babbit F982 Eat 336 Rat 337 0.430 O.440 Av.0.0152 1.750 1.800 1.865 0.265 0.240 Ciiange in Weight fjqpreesed as percentage of Initial Weight During period of xposure After period oi' hxporure - 1.2 - 2-1 * - 1.7 - 1.4 0. - 6.e - 6.0 * r' - 6.6 -19.1 - 9.2 - - 11.9**. -29 *6 7.^ - pate as Hoted at and of Last Period of exposure Died (1) Died (1) 5 Died Died (2) Died (2) Died (2) Died Died survived Died (3> 5 Died .urvired Purrired Died Died Died 3 Length of Surrival i f Animal* Died (D) or Vetf* > Killed <*) j After Last Period of Expoeure i 1 1 * J . e .ijJ 4 -- _____ 9 day* 0 .J4 ? 1 27 day 0 6 dava D -` i j \ 1 ; : NfcV 016520 j 0 / '(*a;e 2) C * i`A l Exper iment Humber Duratlcn of Exposure Hours Identli'cation 2cunber j initial *ei;_:ht *>i. i CI.l>,e in Iimpressed ad -^rccjiiuR-je I of initiiti ..slvilfc during /.rtor eriod ol` ierica oi orare late es rioted at :UlJ cf Last 1eried of -xpoture 1 Lon M i o f Survival o f Anirsals That- . Died (0) or Were ailed (S) After Last foried of Exposure mmmhmhhmmhh ooooooooooo 3 Guinea Fi-. A74 Guinea t i c A?65> g .415 w .3/ ^ 4^1 -j--1v/. vC ..urvived urvivod 7 hours D ;; days D X l-iouso ) .urvived 5 minutes D 1 House > j--V ou16 mm iurvived 1 day D I House ) .urvived 6 days D 1 *oase ) i.urvived 6 days 0 Rabbit FvC3 Rabbit F9C4 Rabbi t F*vC5 Rat 336 1.765 1.765 1.560 fV- <****.(.. ; j 0 0 w \ - 7.4 +.2<6'i.70 o +L15*0 +36.3 Survived urvived :Survived '.urvivod 23 days K 23 days X 23 days K 24 days K > Rat *339 -.265 -10.6 +30.9 survived 24 ays S j 0.33 Guinea ile A7C6 0.460 - 0.7 urvived 1 day D 0.33 Guinea ili; A70y G.4C - -50.0 urvived. 7 days 0 0.33 0.33 5 Mice Sabbit F9C5 v.0.0162 2.075 -22.0 - 1.1 + s. 0 5 -"urvived f:urvivod 1 day D 6 days it 0.33 Rabbit F966 1.364 -- -1 3 .2 :-urvived 10 days D 0.33 Rabbit F97 1.960 - 1.0 - Survived 6 days K 0.33 Rat 340 0.220 0 + 4.5 Survived 13 coys* 1 0.33 Rat 2341 0.225 0 + 5.0 ..urvived 13 days K 715773 NfcV 01t5il xpr- Duration of latent xposure Identification ffuaber hours huriber fable 7 U l * 3) /A Initial .eiiiat h. Chan; e in U'eivht dxpreBsed as fereoutage of Initl al '*eiht During Af ter Period ofi icriod of .xpeaure Ljtposure ^ O *i"i0 N 1 !A L 1 1 r `'ci Fate as lioted at of Last Period of .SsxpoGura Length of Survival 6 of Animals that* Died (D) or Weafc Killed (X) ,,| After Last Period of Exposure 5 6 3.0 Guinea Pi;.; A7'83 0.140 . 3.0 Guinea Pic A769 0.415 3.0 1 4 yice 3.0 1 ;*.ouse ) ;lTT n fV' *' ) -25.5 - 3.0 Rabbit F96C 2.160 - 7.1 3.0 i Rabbit P969 1.695 -3.9 3.0 Rabbit F970 1.670 - 1.1 3.0 Rat 342 0.260 - 4*6 3.0 Rat J2343 0.215 - 4.7 x.o 1.0 Guinea Fig; A790 Guinea i-ig A791 0w.6f,05 - 5.5 - 9.5 1.0 5 Mica Ay .0.0142 0 1.0 Rabbit J971 1*795 0 1.0 Rabbit 7972 1.950 c 1.0 Rabbit F973 1.600 0 1.0 Rat h344 0.330 0 1.0 Rat .3/4.5 0.295 0 -62.0 -63.3 - -23.7 -13.6 +19.7 + 8.1 + 5.0 -11.2 + 7.5 +65.9 +2e..o +36*6. +17.3 + 3.6 +21.6 Survived Survived Died :'orrired urvived Gurvlved Survived Survived Died viurvived Survived 5 6arrived Survived .urvived Survived Survived .urvived 9 days D 10 days O 4 days D 22 days K 22 days X 7 days X 12 days X .; ? ! i i 1.6 months X 1 month X 28 days X 22 days X 1*4 months X 22 days X 23 days X 23 days K 7 i \ j 715774 (1) 2.25 hows i'ro*-;: the start of cxpot ure. \Z) 6 hours frca the start cr exporure. (3) 1*5 hours fror. the start oi closure. NtV 016542 'Fable C O N F iS ^ N 5 `A L The Kortallty Lerv x.h :f Survival, and Chan cs In V.elrht cf Animals deposed to Aerosols termed by Prop,to; Aroclor 12l;S Into r.n Inconel Tube liested to a -criuerature of 1C$0 f . (566 .) W Expor- Duration of inert hjcpofftora Identification Htesber '.lours limber Initial Weight kill* Cban|~e 'Ln .'eirb.t Lxpreasod as iercorxtaije of Init:Lai During AT tor iorioa of j^oriod or Kxposure ^pocure Fate as looted at und of Last foriod of exposure Length of Survival of .Animals That Died (D) or War# Killed (K) < After Last Period of xpoajar# 8 7*0 Guinea i'i A7`>i+ OA35 - 2.3 7.0 Guinea ii^ A7v5 C.i|.60 - 2.2 7.0 7.0 + 7.0 i. Hico 1 bouse ) ) Av.0.0236 -- -- Died Died -- 4 Died mm Survived 7.0 7.0 7.0 7.0 Rabbit x 977 Rabbit Fv7C 2.060 2.200 - 9.2 -10.0 Died -- Died 7.0 Rabbit P979 7.0 ! Rat u36l 7.0 + 7.0 Rat IG362 2.175 0.260 0.295 -- v.2 - 7.7 4 \ -- - -32.5 Died Died Survived 12 hours D mm - 5 days D 7 7.0 Cuinea fir A792 C.Ij.50 0 Died 7.0 Guinea fiii A793 0.1*20 - 1.2 -- Died -- 7.0 2 Mice ) mm -- 2 Died 7.0 1 Mouse 7.0 2 Mice } a v .0.019 } -4.5 - " Survived Survived 5 day* > 26 days. K 7.0 Rabbit Fv7ii 1.965 - C.5 - Survived 5 days J> 7.0 Rabbit F975 1.665 - 9.ii Survived 1*3 soothe K 7.0 Rabbit F976 7.0 Rat *359 7.0 Rat 360 2.005 0.305 0.310 - c.l - 2.6 - 3.2 19-4 - Survived Died Died 1.3 m o n t h K '_ i NEV 016523 715775 Exper Duration of iment Exposure Identification Suratoer Hours Busbar Table 8 (Pa^e 2 ) Initial Weight kg. Chance in weight ojearossed as Porcei*ta?;e of Inicial Weight During After Period of Period of exposure Exposure C `'iP /0 n T i A 0 Fate aa Noted at 2nd of Last Period of Expoeur Length of Survival of Animale That * Died (D) or Were * Killed (K) After Last Period of xpoaura .9 3*5 Guinea Pig A7v6 0.390 Died 3.5 Guinea Pig A757 G .1*15 - 3.5 3 nice ) - 3.5 1 .'louse ) Av.0.019 --_ 3.5 1 House ) ~ 3.5 Rabbit F966 2.320 -13.6 3.5 Rabbit F97 2.250 --- 3.5 irahbit FyGc 2.365 - 0.6 -- -- - +15 - l*-3 Died 3 Died Survived Survived Survived Survived Died -- -- 10 day I> 20 day K 1 month S 5 day 3.5 Rat H363 3.5 Rat 236*4. 0.250 0 .21*0 - 3.4 -10 .0 +23.1 +2 6 .2 Survived Survived 1.1 wonth X 1 .1 Months X 10 1 .0 Guinea Fig; A798 0.1*55 - 2.2 Died 1 .0 Guinea Fig A799 0 .1*30 - 3.5 Survived 2 .6 hours B i 1 .0 5 FJ.ce Av.0.0190 , - 2 .2 +37-9 Survived 1 .0 Rabbit F9o9 2 .01*0 -- >. - 6.9 Survived 20 days 6 days D 1 1 .0 Rabbit F991 2.260 - 3.5 +17.3 Survived 1 Month K 1 .0 Rat 365 0.315 - 1*.8 +21*.8 Survived 1 .1 BKffltfaa X; 'j 1.0 Rat 366 0.305 - 1*.9 +1 6 .1* Survived 11* days X 11 0.33 Guinea FIt. 80 o . U -0 -1*5.1 Survived 1C daya D j 0.33 Guinea Pic A801 c.l*55 -- 1.5 Died - . J 0.33 5 nice Av.0.060 -26.5 +66.5 Survived 19 dayrf X 1 . 0.33 Rabbit F556- 0.33 0.33 Rabbit F99> Rabbit F996 2.095 2.120 2 .1 /0 -1 1 .2 - 3.2 2i*.3 -2 1 .1* -26.1 Survlvod Survived Survived 1 month K 1.3 oaths X 3 12 days D * ] 0.33 Rat 2367 0.280 0 +2 9 .6 arrived 1.1 asmths X i 0.33 Rat 368 0.255 0 Survived 1 1 .1 aonths X f wm i : /-.ir)/ 4 715776 Table S (Pago 3) GQ bigio ENTI AL ?j -1 Exper Duration of iment Exposure IdeatIfication Xiaibw Hours Number Initial Weight kg. Change in Weight Expressed as Percentage of Initial Weight During After Period of Period of Exposure Exposure Fate as iioted at End of Last Period of Exposure Length of Survival of Animal That Died (D) or Vera' Killed <K) After Leat Period of KxpQft .are 12 3.5 Guinea Pig a S02 O.4-6O - 3.3 Died 3.5 Guinea Pig A803 C.450 - 2.2 -- Died - 3.5 5 Mice Ay .0.017 0 22.4 'Survived 16 days X 3.5 Rabbit P997 2.210 - 7.2 +20.5 S Survived 29 days X 3.5 Rabbit F998 2.060 - 5.5 +11.3 Survived 29 day X 3.5 Rabbit P999 1 2.090 3.5 Rat 369 0.255 3.5 Rat 370 0.235 0 0 - 5.7 10.9 26.7 Survived Survived Survived 5 day 0 1 month X 1 month X 13 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Guinea Pig a G04 Guinea Pig A&>5 3 Mice ) 1 House ) Rabbit G2 Rabbit G3 Rabbit Gii. Rat 371 Rat 372 0.42S 0.405 AAtVe*Vn*UmX7f 2.340 2.410 C2..233405 0.215 - 12..04 0 -13.6 - 6.6 - 3.2 - 6.5 - 2.3 - 40.6 24.3 12.4 9.6 7.4 Survived Survived Survived Survived Survived Survived Survived Survived Survived 3 hour D 3 hour D 16 day X 7 day D 37 day X 29 day X 29 days X 32 day X 32 day X t .I1* ; 715777 NfcV 0165^5 Table 9 i ^''V7 rrr*- t1. ^O tr:*fkti --7 j The >k>rtality, Lory ch of Ditrylyal, and Oanlos in el: ht cl* Arizuals -cuosod to Aerosols Foraed. by jpop;,lrt: rlcroryl Phosphate Into art.Inconel Tube Heated to a lobera turo o! 1250 P. (677 C.) i Exper Duration of iment Exposure Identification Stanber Hours Sumter Initial Weight kg. Change in Weight Expreeaed as Percentage of Imti&l *cigci During Period of Exposure After Period of Exposure Pate as toted at .1id cf bast Period of .oxposure Length of Survival of Animals That Died (D) or tere Killed (K) After Last Period of Exposure 15 2 x 7.0 Cat A371 3.611 - .6.5 2 x 7.0 Guinea Pig 662 0.104 - 4.0 7.0 Guinea Pig Ao63 o . m - 2.3 2 x 7.0 5 Mice Av.0.0154 - 2 x 7.0 Rabbit G17S 2.963 - 6.4 2 x 7.0 Rabbit G179 ! 2.543 0 2 x 7.0 Rabbit Gl60 2.791 - 4.9 7.0 Rat tj.79 O.ij.06 - 2.2 7.0 Rat S480 0.450 + 1.2 +11.9 - -- +12.9 -15*6 +19.6 -- -- Survived Survived Died 5 Died Survived Survived turvived Died Died 7 days K. 22 days - 17 days K 14 days D 16 days C e* 2 7.0 7.0 7.0 7.0 7.0 7.0 * 7.0 7.0 Guinea Pig a 8 c 8 Guinea Pig Ai>09 5 Mice Rabbit u-S Rabbit G9 Rabbit 010 Rat K375 Rat K376 0.410 049 5 Av.0.017 1.945 2.010 2.075 0.195 0.225 - e.o- 4.9 - 1.4 - 6.2 - 2.7 0 -14-9 -12.0 -36.0 - +36.6 +22.1 +33.5 - * Survived Died 5 Died Survived Survived Durvlvod Died Died 30 days D -, - 27 days K 27 daya K 27 days , - 715778 NfcV 016526 S xp er- D u ratio n o f irsen t Exposure Id e n tific a tio n 5unber Hours Number Table 9 (are 2) C 0 p *'0 4\* r jA L In itia l w eig h t K Chance in ><ei0.h t .tepreased as p e rc e n ta g e o f I n i t i a l W eight D u rin g P e rio d o f .jc p o c u r A fte r P e rio d o f Exposure P ate as d o ted at End c f Last io rie d o f Exposure Length o f S u r v iv a l o f A n in a l* T h at Died (D ) o r V w * K ille d (X ) A f t e r Last . Period o f Exposure 1 3 .5 G uinea f i g a 806 0 .5 0 0 2*6 D ied 3 .5 G uinea FJL& A307 0.420 - - D ie d 3 .5 2 M ice ) - 2 D ie d -- 3 .5 1 Mouse ) A v .0 .0 1 7 - 10.0 - s u rv iv e d 22 day* D 3 .5 2 .lic e ) + 33.3 S u rv iv e d 34 day* X 3 .5 R a b b it c5 2.270 0 +1M .8 S u rv iv e d 2c days K 3 .5 R a b b it C6 2 .1 1 0 0 + 3 7 .4 S u rvived 28 day* X 3 .5 R a b b it 0 7 2 .1 4 0 0.5 + 2 0 .7 S u rvived 28 days X 3 .5 Rat 373 G .220 0 + 2 .3 S u rvived 34 days K 3 .5 H at 374 0 .2 1 5 0 + 2 .3 S u rvived 34 day X 4 1 .0 G u inea F ig A d 2 2 0 .4 4 5 - 1.6 D ied 1 .0 G uinea r ip Au23 0.430 - 5.3 - D ied 1 .0 5 M ice Av.0 .0 1 5 0 + 5 7 -3 S u rvived 1 .0 R a b b it G22 2 .0 9 5 - 2.0 +32 .2 S u rvived 1 .0 R a b b it G23 1 .9 9 5 | - 2 .3 H -1 9 .5 S u rvived 1 .0 R a b b it 0 2 k 2 .1 1 0 - 5 .9 + 20 .6 S u rv iv e d 1 .0 R at i.399 0 .2 1 5 - 6 .0 + 2 .4 S u rvived 1 .0 R at 400 r 0 .2 1 0 - 3 .8 + 16 .7 S u rv iv e d 6 0 .3 3 G uinea f ir ; A842 0 .9 0 5 - 1 .1 14. - .t1 A1 ' r '- r S u rvived 0 .3 3 G uinea F ir a 843 c.eoo 0 + 3 .9 S u rvived 0 .3 3 5 M ice A v .0 .0 2 2 0 + 1 ,.5 s u rv iv e d 0 .3 3 R a b b it G114 2 .4 5 0 0 + 15 .7 S u rvived 0 .3 3 R a b b it G117 2 .2 > 0 9 .1 + 32 .9 S u rvived 0 .3 3 R ab b it G lie 2.260 - - 5.2 + 7.3 :u rv iv e d 0 .3 3 Rat 437 0 .1 7 5 - 5 .7 + 23.4 S u rvived 0 .3 3 R at 43o 0 .2 0 5 0 +20.4 S u rvived 22 days K 19 days X 12 day* D 28 day* X 1 .1 months X 1 .1 months X 8 day* X 8 days X 11 d a y *.X c day* X 1 .1 nonths X u day* X 8 day* X , 8 day* X 715779 Exper Duration of iment Exposure Identifleafclon Kuaber Hours Number Table 9 (i'c-;e 3) CONFJ At Initial ..'eight k. lw e lii '..eight vcpreciod e5 Percentage of Initi al 'eight T/UriJl Period cf exposure After Period cf Exposure Tate as Doted at .unG of bast Period of Expoeure Length of survival of Animals That Died (D) or Vera Killed (X) After Last Period of Exposure 6 3*5 Guinea Pig. Ac46 1.060 0 3.5 Guinea Pig Ao47 0.770 -7.6 3.5 2 Klee ) _ 3.5 2 Hice ) Av.0.02i( -- 3.5 1 House ) - 3.5 Rabbit G129 2.235 -0.2 3.5 Rabbit G130 1 .9 2 0 . 0 3.5 Rabbit G131 1.965 0 3.5 Rat hljlil 0.315 3.5 Rat 0.335 -7 .5 +13.2 ** -3c. 0 - 7.0 + 0.3 *. C ^ - + 9.6 Survived survived 2 Died Survived Survived !Survived survived Survived Died survived 7 days X. 7 days X -- 7 days D 7 days X 7 days X 7 days X 7 days X 7 days K 7 1.0 Guinea tLi: Aui+4 0.335 0 + 2.1 Survived c oays X 1.0 Guinea tig A645 0.370 0 + 3.1 Survived 8 days X 1.0 5 Mice AV.0.023 -6.0 +16.5 Survived 5 days K 1.0 Rabbit C119 2 .(40 -3.8 +19.1 Survived 25 day X 1.0 Rabbit G120 2.325 0 +10.k Survived 8 days X 1.0 ' Rabbit G121 2.470 -7.3 +35.6 Survived 1.1 months X 1.0 Rat 439 0.195 -5.1 +24 6 Survived 8 days X 1.0 Rat 2(40 C.190 0 +23.7 Survived 6 days X 715780 NEW 016526 TablelO C ^ H iD isT /a t The r ^ o r ta llty Lcn th c f u r v lv a l, and Changes In -.Vel. i t o f A n ia & ls xpoged to A e ro s o ls I or-ned by D ro p p lry. T r ic r o g y l 1hospha'ce In to an In o o n e l Tabe Ilc a to d to a rem ore.i.uie o f 1 0 5 0 F . ($ 6 6 C ) Exper D u ratio n o f im e n t Jcpcsuro Number Hours Id e n tific a tio n Sunber In itia l e ig h t *2 - Change in 'w eight lixpressod as P ercen tag e oi' I n i t i a l U e ir lit D u rin g P e rio d o f Lx^osuro A fte r o ric d o f Exposure F a te as Koted a t uizu o r Last P e rio d o f pjcposure *- Length, o f S u r v iv a l j o f A nim als T h a t i D ie d (D ) o r V e ra K ille d (K ) . A fte r Last P e rio d o f Exposure 7 .0 G uinea p ig A c60: 1.035 - 4 .0 D ied 7 .0 G u in ea Pi,; A661 0.15-63 - 6 .0 -- D ied 7 .0 5 Sice rV0 L`20S -2 3 .1 D ied 2 x 7 .0 R a b b it G163 2 .3 9 3 - 4 .8 - D ied 2 x 7 .0 R a b b it G164 2 .0 2 9 -1 2 .3 - S u rv iv e d 2 x 7 .0 R a b b it G165 2 .0 0 5 - 10.7 ! -- D ied 7 .0 R at E^71 C .454 - 10.1 D ie d 7 .0 R at 472 0 .4 7 4 - 5 .9 ' -- D ie d 0m 0m 1 hour D --1 % 13 7 .0 G u in ea P ir AC50 0 .4 3 0 - 4 .2 7 .0 G u inea P ig AC59 C .4 0 - 1 .2 * 7 .0 7 .0 3 M ice 2 iic e ) J AV.5 016 -- 7 .0 R a b b it (151 7 .0 R a b b it G1S2 7 .0 R a b b it G153 7 .0 B a t <.03 7 .0 R a t 1.64 2 .0 4 0 2 .5 2 5 2.035 0.470 0.455 I - 7 .7 -1 0 .5 - 6.4 - 4 .0 - 2 .2 , - + 16 .9 + 13 .5 . -- D ied D ie d D ied S u rv iv e d S u rv iv e d S u rvived D ie d D ied D ie d . 13 days K 12 days K 13 days X as 0 ** 1 N6V 016529 i 715781 Xable 10 (Page 2) cc. Exper Duration of iment Exposure Identification Number Hours Number Initial Weight kg* Change in Weight Expressed as Percentage of Initial Weight During Period of Exposure After Period of Exposure Pate as uoted at caiu OX Last Period of Exposure Length of Survival of Animal* That ; Died (D) or Ware Silled {KT After Last Period of Exposure 3 3.5 3.5 3.5 * 3.5 3.5 3.5 3.5 3.5 3.5 Guinea Pig A0l8 Guinea Pig A819 1 House ) 3 Mice ) Rabbit G17 Rabbit Gl8 Rabbit G19 Rat E393 Rat E394 0.1)34 0.430 AV arUva*i*5P 2.150 1.6C4 2.180 0.193 0.197 - 1.8 3.3 -19.3 - 1.6 - 2.5 - 2.8 - 4.1 0 A +44-9 -19.0 23.0 +12.2 +20.7 12.7 Died Died Died Survived Survived Survived Survived Survived Survived -- 26 day* X 7 days D 20 days X 20 day* X 25 day* X 25 day* X 10 1.0 Guinea Pi/- a 852 1.110 -11.1 + 3.0 Survived 8 day* X \ 1.0 Guinea Pig A853 1.280 - 3.4 Survived 2 days 0 1.0 3 Klee 1.0 2 Mice ) ) _ Av OeGXo mm -22.2 -8 +19.5 Died Survived 7 day* X 1.0 Rabbit G1U2 2el66 -- -47.8 Survived 26 day* D 1.0 Rabbit Gli>3 2.835 -43.0 Survived 17 day* D j 1.0 Rabbit G144 2.470 - 2.0 6.7 Survived 8 day* X 1.0 Rat E454 0.160 - 5.0 19.4 Survived 9 day* X * 1.0 Rat 455 0.160 - 6.9 - Died i 11 . :V 016530 1 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 Guinea Fig AC54 Guinea Pig A655 5 Mice Rabbit G12-5 Rabbit G146 Rabbit 0147 Rat 2)56 Rat 3)57 0.446 0.465 Ar.0.0190 2.400 2.333 2.395 0.450 0.450 - 5.5 - 1.7 -13.7 - 2.2 - 6*4 0 - 6.0 - 9.1 +15.9 + 5.6 +17.9 3.5 11.6 10.9 + 2.4 17.8 Survived Survived Survived Survived Survived Survived Survived Survived 9 day* X 9 daya X 10 day* X 8 day X 8 day* X 8 'day* X i 9 days X * 9 day X ! ] j j 715782 Table 10 (Pa^e 3) C O l\'.-iD ^T!AL ExpMV Duration of 3want Exposure Identification Buaber Hours Suaber . Initial eight *8- Change in weight Expressed as Percentage of Initial 'eight During Period of Exposure After period of Exposure Pate as Soted at End of Last Period of Exposure Length ofStznrlvaXi of AniwslsMbafc 1 Died (9) or tier* Killed <K) ri After Last Period of Exposure! 3.5 Cuinea Pig a 850 0.920 3.5 3.5 3.5 Guinea Pig AS51 3 Klee ) 2 Mice ) 1.035 AA*Y.AUeAO1X7/ 3.5 Rabbit G139 2.145 3.5 Rabbit C-1L0 3.5 1 Rabbit Glli.1 3.5 Rat 1*44 2.430 20..346600 3.5 Rat 445 0.330 -- 3.0 - 0.2 --2111..49 - 9.0 - 40.9 --106..67 0.9 _ +21.4 + 06.3 + 0.8 + 8.8 Survived Died Died Survived Survived Survived Survived Died Survived 8 days * 7 daye X 9 day* X 14 day X day* X 9 days X 12 1.0 1.0 1.0 1.0 Guinea Pig A856 0.750 Guinea Pig A857 0.510 5 Mice Rabbit Gll|8 a t .0.017 2.668 - 5.2 - 50.3 - 8.1 + 1.2 -17.6 11.8 +10.2 Survived Survived Survived Survived 6 day D 29 days 9 89 days days X X 1.0 Rabbit G149 2.400 - 2.0 +16.4 Survived 6 days X 1.0 Rabbit G150 1.0 Rat 458 1.0 Bat 459 2.170 0.460 0.430 - 1.8 - 4.3 - 6.5 I +15.2 + 3.0 + 4.9 Survived Survived Survived 23 days X 9 days X 9 days X __________ 1 715783 NV 016531 V9JSU of liquid delivery vas about one-half of that in other experinonLs of similar duration i S !9 s: 1s O *1 * * pS* r . g o ri tf 1* *< 2 S I! Eg* ssr o 3 JVSn o to 1 ! 05 r *e I !i M p o o 0 MP rr h* c. 0 VSA tciv O 08 <+ MW O MW-s) w u jv fw o v n o ^ HW O HVJ-v3 to # u O VJ\Wo VXo # #W ->J -s MW OHWsJ O p vr\w o v n o o V &s i* M ow ow M w vn C ^ V ^ jrV iV ? O H O > * 0 to tVJ tototototototo to H O H to toto to to to to tototo O o o to w MU* w w w tJw w Vj titilli O H O O O to to to to to toto to to O O O M bM H to to toto tototo OOCHCOH w w w C iw C X ij o .......................> H o o 0"v>` n K^vivVuw o o p o o to^) to to toto to toto p to to to to to to to'to toto to toto O H H H r o H V..J i i MO 0 p f(Af o *T) fj MM c(*a brt> P i PC' cr H* ? o p K MPiS 0 H IM P a O O V /.. H H H MM M <O H O W W f* >>1CDO'^' H toVMA v to H OvO-J CO JWi (/t9V al as iB * Table 11 *J s K t lA L StngwoTT of Mortality A>aonr< Groups of Anlnals Subjected to Aerosols Fonaed by Dropping; pydraul F-9 Aroclor 12k & or TrlcresTl Hrosphate Into a Heated Inconel Tube Confound and Tasperature or Contact Duration Hours Klee Bata Guinea Figs Rabbits Cats Experiment M mt Pydraul Decoapositlon 1250 F. 6.0 6.25 2/2 1/2 0/2 0 A 6.75 2/2 2/2 0/2 A 3*1 % 2/2 1/2 1/2 o A 0.9 0/2 1/2 0/2 o A 0.25 Va 0/2 0/2 0/2 1 2 3 1 Fydraul Decomposition 1050 F. 6.5 * 6.C 6+8 3*k 0.9 0.25 Arcelor Decomposition 1250 F. 7*0 3.0 1.0 0.33 3.0* 1.0 1 a I I 1/2 2/2 1/2 2/2 0/2 2/2 0/2 2/2 0/2 0/2 2/2 2/2 2/2 2/2 0/2 2/2 0/2 2/2 !/2 2/2 0/2 0/2 2/2 8 8 $ S3 0A o/S o/i a* 6 l 9 10 1 2 6 Ntv 016533 CONFIDENTIAL Tabi 12 IncIdenoo of Mortality In Relation to Material and Spoolee Kor,ardas a of the Toroporaturo of t'-ia Inoonol Kpeoi Total Number of Animala That Diad/iotal 1lumber ixpoaed Iydraul F-9 Triorosyl Aroolor 121*8 Fhosphate :ilca $ 26A9 (27.D % 37/63 (28.7) % 32/69 (ii-6.ii) Guinea Fi^a 13/^0 (62.0) A/26 (92.3) 17/20 (60.7) abbito Kata li/2ii U6.2) 16/38 (1*2.1) 9 A 2 (21.li) /20 (U0.0) 9/26 (3*1.6) 11/28 (39.3) NEV 01653* 715786 r I CONFIDENTIAL fote*J X Incidono of Mortality In Relation to Material* Spoolof. and Tomberaturo of Incone Spool* Ilio Oulna rip* Rabbit* Rat* Total Mio Guinea i'ie* Rabbit* Rat* Total Hloe Guinea Pie* Rabbit* Rat* Total Number of Animal That Diod/iJumber of Animala deposed 1250 F. 1050 F. Aroolor 1?48 % ?i\JZ9 (82.8] 10/12' (03.3! 7/18 (30.9; 5/12 (Itl.7) L6/71 (6/1.8]1 % 1143//1314; (38.2) (100.0) 9/20 (45.0) 4/14 (20.6) 40/82 (48.8) fydraul F~9 15/24 5/10 1/10 6/10 % (62.5) (50.0) (10.0) (60,0) 27/51; (50.0) 13/25 8/10 3/14 2/10 (52.0) (80.0) 21,4) (20.0) 26/59 (44.D Trioray1 Phoaphnt 17/35 7A1; 2/21 5/11; % (L0.6| (50.0 ( 9.5 (35.7] 31/01; (36.9]I 15/34 10A4 7/21 6/14 % (44.1) (71.4) (33.3) (42.9) 30/83 .(45.8 715787 NEV 016535 iteblo '14- 'fho 'toxicity of fr:;draul I-9 when hai; tali.ou >orilnucusl" for Twonty-fo^r lours In Contact `With the f.klr. of hobbits by the ?leore Method of Oraize, Soodard sa j Colvery (Applied undiluted) Identi fication imniber Condition of Skin Dos iiila Ini Lial a Lr^t k* Loss of Weight dxprfccced as fercenta,,_e of Initlel Weight i-a '-e Length of Survival After the Material fas Applied G-333 0-339 0-340 0-419 0-421 0-420 0-438 0-439 Intact Intact Intact Intact Intact Intact Intact Intact 9*4 2*060 9*4 2.390 66..00 2.275 1.630 6.0 2.016 3.6 2.100 3.6 2.262 3.6 2.265 0-455 Abraded 6.0 2.090 0-45? Abraded 6.0 1*966 0-458 Abraded 6.0 1.666 36.1 17*4 19.8 9.9 9.6 0.1 3.8 3.7 36.5 4.3 15.2 Died Died 7 days 3 days Diedi 20 days Survived 9 days; killed Survived 9 days; killed Survived 23 days; killed Survived 16 days; killed Survived 16 days; killed Died Died Died 19 days 44 hours 3 days NtV 016536 Table 15 f 3 = N TI A L Tho Toxicity of Aroclor 124o When Malnta.ir.ed Continuously for Twenty-four Hour' in Contact With the Skin of Rabbits J2X the Sleeve Method of Praise, Woodard and Calvary (Applied undiluted} Lose of 'weight Identi Initial Depressed as fication Condition Dose Veight Percentage of ftuzaber of Skin sal./ko* kg. Initial Weight Fate Length of Survival After the Material Was Applied c-348 g -44o G-iiU Intact Intact Intact 9.4 2*310 9.4 2.310 9*4 2.053 0-^22 Abraded 9.4 1.C65 0-424 Abraded 9.4 1.922 0-443 Abraded 9.0 1.970 c-444 Abraded 6.0 2.196 0-456 Abraded 6.0 1.862 19.3 3.9 26.2 9.2. 35.2 14.8 19.2 12.7 Survived 21 day; killed Survived 21 days; killed Survived 31 day; killed Survived 44 days; killed Died * i* day Survived 29 days; killed Died Died 6 day 21 hour NV 016t>37 Table 16 : <D - N 7 ! A L The Toxicity :-f Trlcrosyl rh&sylmto ';u\on -Ie.iutain.od Continuously For Tweaty-four Hours In Contact With the Skin of babbits by the Sleeve Method of Praise, v/oodard and Calvery . (Applied undiluted) Identic fleetion umber Condition of Skin Dose tnl./kg. Initial Weight kg* Loss of Weight Kxpressod a0 percentage of Initial Weight Fat Length, of Survival After the Materiel Was Applied 0-628 G-337 0-331i c-m 0-345 G-U23 0-445 Intect Intact Intact Intact Intact Intact Intact Intact 9.4 2.487 6.0 2*460 2-5 2.190 2*5 2*360 2.5 2.125 1.6 1.620 1.6 1.609 1.6 2.158 15*5 16.1 21.a 36.2 9.8 13.2 11.3 5*9 Died 2 days Died 2 days Survived 20 days; killed Died 7 day* Survived 6 day*; killed Survived 9 days; killed Survived 21 days; killed Survived 16 days; killed NtV 016i>3tt a llo 17 C o is ? l O Z I A T I A L Xho toxicity or Car, oyle DTK When lalntaired ucnii/uot-tily or Twenty-four Hours In Contact Vlfcfr the :kin oi' CobbIts by the -'loeve Method of Praise, Woodard and Ct-.lvery (Applied undiluted) floation. Condi bion Dose Number of Skin al.A* Initira Weinat Lose of weiiiit jenpressod as io-reonta^o of Initial Weight I*GLwC Lonrth of Survival Alter the Material Has Applied G-329 c-331 0-341 a-342 0-343 Intact Intact Intact Intact Intact 9.4 2.470 9.4 2.345 9.4 2.030 9.4 2.210 9.4 2.160 0-332 Abraded 9*4 2.14 0 G-352 Abraded 9.4 2.450 G-354 Abraded 9 .4 2.255 12.9 11.9 1: .2 17.6 10.2 v 6.6 10.4 20.2 Survived ourvlvod survived Survived Survivod Li* days; killed lb days; killed 20 days; killed 19 days; killed 8 days; killed survived 9 cays; killed Survived 20 dayc; killed Survived 20 days; killed 715791 NV 016539 ia'olo iS P O N f i D e n t ,A L ouao&rizod Saba on the Ir-t:j-</ ci3LCiX/<D ToxicIt;; of PyJ-raul F-9 A ro c lo r 12h8 TrloresyI Phosphate and Gargoyle DiS tlien Maintained Upon the Skin of Rabbits According to the Sloeve /-othod of Pralae, Woodard and Calvery Dosage nl/kg. liunbcr that Jied/iftcciber Given the Material pydr&ul P-9 Aroclor 12^.8 Tricresyl Phosphate Gargoyle DTK Intact Abraded Intact Abraded Intact Intact Abraded 9 A 2/2 0/3 1/3 i A o/5 0/3 6.0 1/3 3/3 2/2 i/l -- 3.6 9/3 - mm * - - 2.5 - - - - 1/3 - - 1.6 - - - 0/3 - -- r Ntv 016540 NEV 016542 016543 715795 4F i g u r e NtV 0 1 6 5 * 4 13 % *0 K 1 Percent Transmsson Figure 5 0i6!><5 } too Per cent Transmisson Figure 6 * -4 jj NfcV )1 1 6 5 ^ 6 r- - r T Per cent Transmisson !t i ~ cn CO CO Figure 8 NEV 016i><7 r. Percent Transmisson Concentration of Tricresyl Phosphate in Ethyl Alcohol,r/mf. Figure 7 N fcV  "'flr*- v Per cent Transmisson Figure 9 N6V 016549 715801 Per cent Tronsmisson 4 'i I Figure 10 NfcV 016330 cn ro ri JfV 1 715803 FIGURE 11. NfcV 016551