Document JrjeZrNGrjyrzv6E3k3YqQ5wB
`AptpoTeNO-nrepdortiofx 31.02
Final version
CONFIDENTIAL
04.12.2008
APPENDIX TO TNO-REPORT OF STUDY 3M-02
Project / study number: 031.10074/3M-02
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In assignment of:
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3M, Medical Department
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JStouhdynmBourtieonrhof,Ph.D. CiH, DABT
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E-mail jbutenhofi@cmomm
`OrS.tuLdoyudiisreHc.toCr:ohen Status and Date:
Final version: 04 December 2006
PDrreav"iou1s6vOerasticonbse:r2008 Dra. 30October2008 Numberofpages: 172
Authorization by Dr. P.H.A. Quax,
Technology manoafVgasceulrar and Metabolic Diseases:
Signature and date: \\
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Page A1 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Contents Contents
page A02
A. Code E3L mice before and after matching
A03
B. Bodyweight and food intake measurements
A04
B1. Individual bodyweights
A04
B2. Food intake measurements
A07
C. Plasma measurements
A13
C1. Plasma cholesterol measurements
A13
C2. Plasma triglyceride measurements
A15
C3. Pooled lipoprotein profile measurements
A17
C4. Pooled plasma ALAT measurements
A24
C5. Plasma ketone bodies measurements
A25
D. Measurements in metabolic cages
A28
D1. Code mice and metabolic cages
A28
D2. Average bodyweight mice in metabolic cages
A28
D3. Food and drink intake measurements
A29
D4. X activity measurements
A38
D5. O2 consumption,CO2 production and respiratory
exchange ratio measurements
A41
D6. Energy expenditure
A50
E. Fecal bile acid, sterol and fatty acid measurements
A53
E1. Fecal weight measurements, bodyweight and food intake
Measurements
A53
E2. Fecal bile acid measurements
A60
E3. Fecal sterol measurements
A83
E4. Fecal fatty acid measurements
A107
F. Intestinal cholesterol absorption
A122
G. Liver weight, perigonadal fat weight and lipid contents liver G1. Liver weight and perigonadal fat weight G2. Lipid contents liver
A127 A127 A129
H. HMG-CoA reductase and cholesterol-7-hydroxylase activity
in liver microsomes and sterol-27-hydroxylase activity in liver
mitochondria
A136
H1. HMG-CoA reductase and cholesterol-7-hydroxylase activity
in liver microsomes
A136
H2. Sterol-27-hydroxylase activity in liver mitochondria
A148
I.
Measurements in vitro experiment (rat hepatocytes)
A156
Page A2 of A172
Appendix to TNO-report of 3M-02
Final version
Appendix A. Code E3L mice before and after matching
mouse#
cage#
before matching
658
86
663
86
712
93
716
93
752
98
758
98
763
99
784
102
790
102
791
102
641
84
645
84
646
84
674
88
737
96
738
96
740
96
760
99
765
99
785
102
650
85
678
88
690
90/91
699
90/91
729
95
754
98
756
98
759
98
766
99
793
103
681
89
686
89
704
92
705
92
706
92
710
92
768
100
771
100
792
103
795
103
665
87
666
87
667
87
671
87
679
88
700
90/91
730
95
731
95
744
97
799
103
714
93
721
94
727
94
769
100
774
100
776
101
778
101
782
101
783
101
797
103
652
85
657
86
670
87
680
89
687
89
696
90/91
720
94
724
94
761
99
762
99
excluded (655) 85
excluded (656) 86
excluded (741) 96
excluded (742) 96
excluded (748) 97
eartag LR
01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
12 00 21 02 20
group#
1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7
mouse# after matching
1 1 2 2 3 3 4 5 5 5
6 6 6 7 8 8 8 9 9 10
11 12 13 13 14 15 15 15 16 17
18 18 19 19 19 19 20 20 21 21
22 22 22 22 23 24 25 25 26 27
28 29 29 30 30 31 31 31 31 32
33 34 35 36 36 37 38 38 39 39
cage#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70
04-12-2006
Page A3 of A172
Appendix to TNO-report of 3M-02
Final version
Appendix B. Bodyweight and food intake measurements
B1. Individual bodyweights
Group
Mouse# cage#
run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in run in
AVERAGE SD
run in run in run in run in run in
641
84
645
84
646
84
650
85
652
85
657
86
658
86
663
86
665
87
666
87
667
87
670
87
671
87
674
88
678
88
679
88
680
89
681
89
686
89
687
89
690
90/91
696
90/91
699
90/91
700
90/91
704
92
705
92
706
92
710
92
712
93
714
93
716
93
720
94
721
94
724
94
727
94
729
95
730
95
731
95
737
96
738
96
740
96
744
97
752
98
754
98
756
98
758
98
759
98
760
99
761
99
762
99
763
99
765
99
766
99
768
100
769
100
771
100
774
100
776
101
778
101
782
101
783
101
784
102
785
102
790
102
791
102
792
103
793
103
795
103
797
103
799
103
excluded (655) 85 excluded (656) 86 excluded (741) 96 excluded (742) 96 excluded (748) 97
eartag LR 10 21 02 01 20 10 01 12 10 01 11 02 12 01 02 12 00 10 02 12 01 00 11 20 00 10 01 02 00 01 20 00 10 20 12 10 01 11 10 01 20 00 00 01 20 02 12 00 10 01 11 21 02 00 10 11 02 00 01 02 22 00 10 02 12 00 10 11 21 12
12 00 21 02 20
bodyweight (g)
week -2 week -1 week 0
30.0
30.1
29.2
27.5
28.1
28.7
31.2
30.9
30.6
27.5
27.6
28.5
29.9
30.0
31.1
31.4
30.9
32.2
32.6
31.8
31.9
30.1
30.9
31.1
30.5
30.7
31.3
25.0
25.6
25.4
28.0
27.7
27.9
30.7
31.0
31.3
28.6
27.9
29.9
31.5
31.4
31.9
29.9
30.0
30.3
29.3
29.0
30.9
31.4
31.5
31.5
29.4
29.2
30.9
29.1
28.9
29.1
29.9
29.6
31.5
31.4
31.0
31.8
26.2
26.3
26.7
27.6
27.9
27.8
29.7
29.4
29.5
29.0
29.7
29.2
31.4
30.9
30.4
32.0
31.9
31.8
24.6
24.8
26.4
29.4
29.1
29.8
29.0
29.6
30.2
28.8
28.4
28.9
28.2
28.8
29.1
32.5
32.6
31.8
27.5
27.1
29.0
26.5
26.9
28.0
25.0
25.3
27.0
29.4
29.4
30.7
28.8
28.5
29.3
28.9
29.0
30.0
28.1
28.7
28.3
30.6
30.9
30.9
27.0
27.4
27.7
29.0
29.4
30.5
28.8
29.0
31.1
28.2
28.0
29.0
27.8
27.3
29.3
24.5
24.7
26.1
25.2
25.7
26.4
23.1
23.8
24.0
25.5
25.9
25.5
25.4
25.0
25.9
27.2
26.8
26.5
28.0
28.1
27.9
27.0
27.6
28.9
28.1
28.2
30.4
29.0
29.3
29.8
27.2
27.1
28.0
25.5
25.7
25.3
28.2
28.3
28.1
27.5
27.6
29.0
27.1
27.0
29.5
28.6
28.9
28.9
29.3
29.7
28.8
28.8
28.7
29.0
26.1
26.8
26.6
27.4
27.5
26.0
27.7
27.8
27.9
26.8
26.7
26.9
29.0
29.5
28.0
29.1
29.4
29.5
28.43
28.51
29.03
2.03
1.93
1.93
27.3
27.0
28.5
22.2
22.9
24.6
29.0
29.2
30.9
31.0
31.6
32.2
30.6
30.9
31.0
04-12-2006
Page A4 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group Mouse# cage#
1
1
1
1
2
1
1
3
2
1
4
2
1
5
3
1
6
3
1
7
4
1
8
5
1
9
5
1
10
5
AVERAGE
SD
2
11
6
2
12
6
2
13
6
2
14
7
2
15
8
2
16
8
2
17
8
2
18
9
2
19
9
2
20
10
AVERAGE
SD
3
21
11
3
22
12
3
23
13
3
24
13
3
25
14
3
26
15
3
27
15
3
28
15
3
29
16
3
30
17
AVERAGE
SD
4
31
18
4
32
18
4
33
19
4
34
19
4
35
19
4
36
19
4
37
20
4
38
20
4
39
21
4
40
21
AVERAGE
SD
5
41
22
5
42
22
5
43
22
5
44
22
5
45
23
5
46
24
5
47
25
5
48
25
5
49
26
5
50
27
AVERAGE
SD
6
51
28
6
52
29
6
53
29
6
54
30
6
55
30
6
56
31
6
57
31
6
58
31
6
59
31
6
60
32
AVERAGE
SD
7
61
33
7
62
34
7
63
35
7
64
36
7
65
36
7
66
37
7
67
38
7
68
38
7
69
39
7
70
39
AVERAGE
SD
eartag LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
week 0
31.9 31.1 29.8 28.9 30.5 29.3 25.9 28.9 29.0 26.6
29.19 1.85
29.2 28.7 30.6 31.9 30.0 28.3 30.9 26.4 26.5 28.8
29.13 1.80
28.5 30.3 31.8 27.8 27.0 31.1 29.0 26.1 27.9 27.9
28.74 1.82
30.9 29.1 29.2 30.4 31.8 26.4 28.9 29.8 26.0 26.9
28.94 1.95
31.3 25.4 27.9 29.9 30.9 29.5 30.7 29.3 27.7 29.5
29.21 1.78
30.2 31.8 28.0 30.4 28.0 25.3 28.1 29.0 29.5 28.0
28.83 1.79
31.1 32.2 31.3 31.5 31.5 26.7 29.1 29.0 24.0 25.5
29.19 2.88
week 1
31.4 29.8 30.2 29.4 30.0 28.4 25.8 29.7 28.9 26.2
28.98 1.76
29.4 28.5 30.7 31.7 30.2 28.2 31.6 25.9 26.3 28.1
29.06 2.03
27.4 29.2 32.4 27.8 26.3 30.8 30.1 26.4 27.5 27.8
28.57 2.00
30.5 29.3 28.6 31.0 31.6 25.6 28.3 30.3 27.5 26.1
28.88 2.04
29.5 24.7 26.9 29.4 29.4 28.4 27.9 27.9 26.3 28.0
27.84 1.53
29.2 31.2 27.1 30.8 28.0 24.8 27.8 27.7 29.8 27.5
28.39 1.91
30.1 30.2 28.8 30.6 30.4 26.2 28.2 28.4 23.4 25.2
28.15 2.46
week 2
32.6 29.8 29.8 28.6 30.9 27.8 24.8 29.0 29.8 26.3
28.94 2.24
29.8 28.9 30.3 32.3 29.7 29.4 31.7 25.9 26.5 28.8
29.33 2.00
27.3 29.7 32.0 28.6 26.1 30.0 29.9 25.3 27.8 27.4
28.41 2.03
30.2 29.2 29.0 31.6 31.4 25.6 29.1 30.7 28.0 26.3
29.11 2.02
25.6 23.8 26.8 25.4 26.9 26.7 28.3 25.8 24.0 26.1
25.94 1.36
28.8 31.7 27.6 30.9 28.4 24.9 28.1 28.4 30.4 27.2
28.64 1.98
27.0 26.6 26.6 28.9 28.4 25.0 26.6 26.0 22.4 23.6
26.11 2.00
week 2.3
25.0 23.8 26.6 25.0 27.0 26.5 28.4 25.6 24.6 25.3 25.78 1.35
26.9 26.4 25.2 27.7 27.3 24.5 25.6 25.4 21.7 23.6 25.43 1.82
week 3
32.5 29.8 29.6 27.9 30.5 28.1 25.0 28.6 29.7 26.2
28.79 2.15
30.2 29.0 29.3 31.7 30.2 29.6 31.7 25.6 26.8 28.2
29.23 1.96
27.1 28.9 32.6 28.8 25.6 29.1 29.9 24.9 27.8 27.3
28.20 2.20
29.7 29.2 28.9 30.8 31.1 25.7 28.3 30.2 27.4 27.0
28.83 1.74
25.6 24.8 27.0 25.7 27.9 27.9 28.4 28.0 25.4 27.0
26.77 1.30
28.7 31.4 27.1 30.5 28.1 25.1 27.7 28.2 30.0 27.1
28.39 1.85
26.2 25.0 24.7 27.8 27.1 24.6 25.5 25.3 22.3 23.5
25.20 1.61
week 3.4
28.0 24.8 27.3 27.5 29.1 28.6 29.5 29.7 25.2 27.6 27.73 1.67
26.0 24.1 25.0 27.7 26.8 25.2 26.2 26.0 23.1 24.3 25.44 1.37
week 4
32.6 29.9 29.8 27.9 30.4 27.6 25.7 28.1 29.2 26.1
28.73 2.08
31.0 28.9 29.4 31.9 30.2 29.4 31.8 26.4 27.0 28.3
29.43 1.87
27.8 29.0 32.5 29.2 25.2 29.2 30.0 25.0 28.9 26.7
28.35 2.27
29.7 28.9 28.9 30.9 31.1 25.3 28.2 30.6 27.5 27.3
28.84 1.83
27.5 24.2 26.7 28.1 28.9 28.4 29.2 29.3 25.7 27.8
27.58 1.63
28.3 31.3 27.1 30.3 28.8 25.1 27.9 28.9 30.6 27.2
28.55 1.87
26.3 24.7 25.3 27.9 27.1 25.3 25.8 26.1 22.8 23.8
25.51 1.50
Bodyweight (g)
week 5 week 5.3 week 5.6
32.9
33.9
33.9
30.5
31.2
30.5
30.7
30.4
31.0
28.7
28.1
28.2
31.1
31.1
30.6
29.6
29.5
29.4
26.5
26.7
27.2
30.0
30.4
30.6
30.5
30.6
31.0
27.1
27.2
27.3
29.76
29.91
29.97
1.90
2.14
2.02
31.4
33.0
33.1
29.4
29.8
30.0
29.9
31.2
32.0
32.9
32.8
33.1
31.0
30.8
31.0
30.2
30.1
29.8
32.9
32.8
32.8
26.2
26.5
26.6
26.6
26.9
26.6
28.6
29.0
28.8
29.91
30.29
30.38
2.31
2.33
2.48
28.2
27.8
28.3
28.8
28.8
28.9
32.9
32.2
32.3
28.9
28.8
28.9
25.8
25.8
26.3
29.0
28.7
28.5
30.2
30.1
30.1
25.1
25.0
24.7
29.2
29.0
29.7
27.1
27.0
27.0
28.52
28.32
28.47
2.21
2.07
2.12
30.2
30.3
30.2
29.3
29.3
29.2
28.8
29.2
28.9
30.4
30.9
30.7
30.7
31.0
30.7
25.3
25.6
25.8
27.3
27.0
26.7
30.2
30.2
30.2
27.1
27.7
27.2
27.5
27.7
27.7
28.68
28.89
28.73
1.80
1.82
1.78
28.6
28.9
30.0
25.0
25.2
25.3
27.4
27.6
27.8
28.2
28.5
28.1
29.2
28.6
29.7
28.2
28.5
28.6
30.3
30.3
30.1
29.3
29.3
29.9
26.5
27.3
27.2
28.0
27.7
28.7
28.07
28.19
28.54
1.51
1.37
1.52
28.5
28.4
28.5
31.5
31.3
31.5
27.4
27.3
27.2
30.3
30.2
30.0
28.7
28.8
28.4
25.5
25.6
25.4
27.6
27.6
27.8
28.9
29.1
29.1
30.4
30.3
30.7
26.8
26.7
26.7
28.56
28.53
28.53
1.82
1.77
1.87
28.0
28.2
27.7
27.2
27.3
26.7
26.1
26.2
26.6
28.3
28.2
28.5
27.4
27.4
28.2
26.1
26.1
26.1
26.2
26.0
26.2
26.5
26.7
26.9
22.8
23.6
23.0
24.6
25.1
24.6
26.32
26.48
26.45
1.64
1.42
1.65
week 6
32.9 30.1 30.0 28.3 29.2 30.4 26.3 30.0 30.5 27.0
29.47 1.89
32.2 30.0 31.2 33.6 30.1 30.0 32.5 26.3 26.9 29.0
30.18 2.34
28.4 29.2 32.3 29.2 26.1 28.8 29.8 25.1 29.4 27.6
28.59 2.00
29.6 28.7 29.6 30.5 30.7 26.2 26.9 30.1 27.2 27.7
28.72 1.62
29.1 25.5 28.6 28.8 30.1 28.6 30.1 29.7 27.0 28.4
28.59 1.42
28.7 31.4 27.0 29.7 28.0 25.3 26.9 28.8 30.9 26.3
28.30 1.99
29.1 27.8 26.8 28.9 27.6 26.2 26.7 27.2 23.6 25.7
26.96 1.60
week 6.3
33.2 30.2 30.2 28.4 31.1 29.6 26.5 30.8 30.7 27.1
29.78 1.99
32.8 30.0 31.8 33.1 30.3 30.9 32.7 25.9 26.5 29.1
30.31 2.53
29.1 29.2 32.5 29.3 26.4 29.1 30.2 25.4 29.6 27.8
28.86 1.98
29.9 29.2 29.8 30.5 31.1 26.5 28.0 30.9 27.5 27.5
29.09 1.61
29.8 25.6 29.2 29.1 29.6 28.6 30.6 30.2 27.3 28.6
28.86 1.48
28.7 32.1 27.4 30.2 28.5 25.6 27.2 28.6 30.6 26.6
28.55 1.98
28.5 27.8 27.0 28.7 28.2 27.1 26.7 27.3 23.8 25.4
27.05 1.50
week 7
33.5 30.6 30.5 28.5 30.8 29.8 27.0 30.9 31.0 27.6
30.02 1.90
33.1 29.5 32.0 33.0 30.4 29.5 31.4 26.2 27.7 29.5
30.23 2.23
29.9 30.1 33.2 29.3 27.2 29.4 30.3 26.3 30.4 28.9
29.50 1.87
29.4 29.4 30.0 30.5 30.7 26.4 27.1 30.7 27.9 28.3
29.04 1.54
30.5 25.9 29.6 29.1 30.6 30.2 30.0 30.5 27.2 29.0
29.26 1.57
28.9 32.0 27.6 30.0 28.1 25.5 26.7 28.5 30.4 26.3
28.40 2.01
28.6 28.4 26.8 28.6 28.3 27.2 26.9 27.1 24.0 25.2
27.11 1.53
week 8
33.8 31.2 30.2 28.6 31.0 29.7 27.5 31.0 30.7 27.3
30.10 1.94
34.1 29.5 32.2 33.7 29.9 30.5 31.2 26.4 28.7 29.7
30.59 2.32
30.2 30.0 33.1 29.5 27.0 30.1 30.1 26.4 30.8 28.6
29.58 1.91
29.7 29.0 30.2 30.3 30.8 26.5 27.6 30.9 28.1 28.5
29.16 1.47
30.4 25.6 29.1 29.1 30.4 29.9 30.4 30.6 27.7 29.1
29.23 1.56
28.8 32.1 26.8 30.3 28.5 25.6 26.7 28.3 30.1 26.5
28.37 2.03
29.0 28.6 27.1 29.1 28.6 27.3 27.2 27.6 24.3 25.7
27.45 1.53
week 8.4
33.7 31.0 30.3 28.5 30.8 29.9 27.3 31.9 30.6 27.1
30.11 2.03
33.7 29.5 33.1 33.5 30.8 31.0 31.7 26.5 28.9 29.5
30.82 2.29
30.5 30.1 33.2 29.3 27.2 31.0 30.3 26.1 31.1 28.5
29.73 2.05
29.0 28.7 29.8 30.4 30.7 26.4 27.2 30.6 27.6 28.4
28.88 1.50
30.4 25.4 29.1 29.2 30.1 29.8 30.7 30.5 28.1 28.9
29.22 1.57
28.5 32.1 26.7 28.1 27.4 25.5 26.8 28.2 30.2 26.3
27.98 1.96
29.4 28.7 27.8 29.5 28.6 27.4 27.5 27.6 24.7 26.3
27.75 1.45
week 9
34.5 31.1 30.0 28.4 31.2 30.0 27.7 33.1 30.4 27.0
30.34 2.32
34.8 30.1 33.6 33.6 31.2 31.2 32.4 27.8 29.8 30.1
31.46 2.14
30.6 30.0 33.4 29.4 27.2 31.4 30.7 26.3 31.6 28.1
29.87 2.17
28.4 28.1 29.6 30.2 30.9 26.4 27.0 30.4 27.2 28.6
28.68 1.55
30.2 25.0 28.9 29.3 29.6 29.7 31.0 30.4 28.5 29.0
29.16 1.64
28.4 32.2 26.6 27.8 27.1 25.7 26.7 27.9 30.0 26.3
27.87 1.95
29.7 29.2 28.2 29.8 28.9 27.8 27.6 28.0 25.0 26.8
28.10 1.45
week 10
34.3 31.4 30.5 28.9 32.0 29.3 28.0 33.2 31.7 27.4
30.67 2.26
35.4 30.6 33.3 33.8 30.6 30.7 32.9 28.3 30.8 30.1
31.65 2.12
31.0 30.9 33.8 28.4 27.3 30.9 31.3 25.9 32.7 27.7
29.99 2.54
28.2 28.1 29.2 29.6 30.3 26.2 26.7 29.7 26.8 28.1
28.29 1.41
31.3 25.4 29.6 29.4 29.5 30.0 31.2 30.7 28.4 29.0
29.45 1.70
27.8 31.3 26.2 27.7 26.8 25.2 26.7 27.6 29.9 26.0
27.52 1.85
29.8 29.5 28.0 29.8 29.5 27.9 27.5 28.1 25.1 27.6
28.28 1.45
Page A5 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0 1 2 3 4 5 5.3 5.6 6 6.3 7 8 8.4 9 10
week
0 1 2 3 4 5 5.3 5.6 6 6.3 7 8 8.4 9 10
week
0 1 2 2.3 3 3.4 4 5 5.3 5.6 6 6.3 7 8 8.4 9 10
group 1 vs 2 nn p 10 10 0.739 10 10 0.912 10 10 0.796 10 10 0.631 10 10 0.436 10 10 0.971 10 10 0.739 10 10 0.739 10 10 0.579 10 10 0.579 10 10 0.971 10 10 0.739 10 10 0.529 10 10 0.247 10 10 0.393 group 2 vs 4 nn p 10 10 0.912 10 10 0.853 10 10 0.796 10 10 0.684 10 10 0.529 10 10 0.280 10 10 0.247 10 10 0.190 10 10 0.165 10 10 0.218 10 10 0.218 10 10 0.165 10 10 0.035 10 10 0.009 10 10 <0.001 group 3 vs 7 nn p 10 10 0.481 10 10 0.971 10 10 0.023
10 10 0.003
10 10 0.011 10 10 0.029 10 10 0.043 10 10 0.015 10 10 0.052 10 10 0.023 10 10 0.003 10 10 0.019 10 10 0.043 10 10 0.052 10 10 0.123
group 1 vs 3 nn p 10 10 0.481 10 10 0.631 10 10 0.579 10 10 0.481 10 10 0.631 10 10 0.143 10 10 0.105 10 10 0.123 10 10 0.190 10 10 0.165 10 10 0.280 10 10 0.353 10 10 0.684 10 10 0.853 10 10 0.481 group 2 vs 5 nn p 10 10 0.796 10 10 0.143 10 10 0.001 10 10 0.005 10 10 0.029 10 10 0.043 10 10 0.035 10 10 0.075 10 10 0.089 10 10 0.075 10 10 0.039 10 10 0.190 10 10 0.075 10 10 0.015 10 10 0.035 group 4 vs 5 nn p 10 10 0.631 10 10 0.280 10 10 0.002
10 10 0.009
10 10 0.165 10 10 0.393 10 10 0.315 10 10 0.579 10 10 0.796 10 10 0.631 10 10 0.853 10 10 0.739 10 10 0.529 10 10 0.353 10 10 0.089
Bodyweight, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn p nn p nn
p
nn p
10 10 0.853 10 10 0.796 10 10 0.529 10 10 0.631
10 10 0.971 10 10 0.075 10 10 0.393 10 10 0.631
10 10 0.796 10 10 0.004 10 10 0.631 10 10 0.011
10 10 0.912 10 10 0.019 10 10 0.739 10 10 0.001
10 10 0.912 10 10 0.218 10 10 0.912 10 10 0.002
10 10 0.247 10 10 0.029 10 10 0.165 10 10 0.001
10 10 0.218 10 10 0.063 10 10 0.143 10 10 0.002
10 10 0.143 10 10 0.075 10 10 0.165 10 10 <0.001
10 10 0.481 10 10 0.218 10 10 0.218 10 10 0.005
10 10 0.481 10 10 0.190 10 10 0.247 10 10 0.007
10 10 0.165 10 10 0.190 10 10 0.075 10 10 0.003
10 10 0.247 10 10 0.280 10 10 0.063 10 10 0.004
10 10 0.143 10 10 0.247 10 10 0.029 10 10 0.023
10 10 0.105 10 10 0.247 10 10 0.019 10 10 0.019
10 10 0.029 10 10 0.315 10 10 0.003 10 10 0.035
group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
nn p nn p nn
p
nn p
10 10 0.579 10 10 0.579 10 10 0.739 10 10 0.529
10 10 0.353 10 10 0.481 10 10 0.631 10 10 0.853
10 10 0.353 10 10 0.005 10 10 0.393 10 10 0.007
10 10 0.280 10 10 <0.001 10 10 0.353 10 10 0.105
10 10 0.353 10 10 <0.001 10 10 0.684 10 10 0.393
10 10 0.247 10 10 0.001 10 10 0.631 10 10 0.631
10 10 0.123 10 10 0.001 10 10 0.393 10 10 0.796
10 10 0.105 10 10 0.002 10 10 0.579 10 10 0.912
10 10 0.075 10 10 0.004 10 10 0.684 10 10 1.000
10 10 0.105 10 10 0.009 10 10 0.579 10 10 0.912
10 10 0.089 10 10 0.003 10 10 0.912 10 10 0.853
10 10 0.052 10 10 0.002 10 10 0.853 10 10 0.853
10 10 0.011 10 10 0.002 10 10 0.315 10 10 0.436
10 10 0.002 10 10 <0.001 10 10 0.218 10 10 0.353
10 10 <0.001 10 10 <0.001 10 10 0.105 10 10 0.579
group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn p nn p nn
p
nn p
10 10 0.796 10 10 0.631 10 10 0.684 10 10 0.631
10 10 0.529 10 10 0.529 10 10 0.684 10 10 0.393
10 10 0.529 10 10 0.007 10 10 0.002 10 10 0.796
10 10 0.853
10 10 0.579 10 10 <0.001 10 10 0.043 10 10 0.023
10 10 0.007
10 10 0.579 10 10 0.001 10 10 0.353 10 10 0.011
10 10 0.912 10 10 0.011 10 10 0.579 10 10 0.015
10 10 0.529 10 10 0.009 10 10 0.796 10 10 0.007
10 10 0.796 10 10 0.015 10 10 0.912 10 10 0.007
10 10 0.739 10 10 0.035 10 10 0.631 10 10 0.035
10 10 0.481 10 10 0.019 10 10 0.529 10 10 0.005
10 10 0.353 10 10 0.029 10 10 0.190 10 10 0.003
10 10 0.315 10 10 0.035 10 10 0.165 10 10 0.005
10 10 0.165 10 10 0.143 10 10 0.089 10 10 0.019
10 10 0.165 10 10 0.481 10 10 0.063 10 10 0.063
10 10 0.190 10 10 0.912 10 10 0.035 10 10 0.075
group 2 vs 3 nn p 10 10 0.529 10 10 0.481 10 10 0.353 10 10 0.190 10 10 0.247 10 10 0.143 10 10 0.052 10 10 0.089 10 10 0.075 10 10 0.105 10 10 0.393 10 10 0.436 10 10 0.280 10 10 0.165 10 10 0.436 group 3 vs 6 nn p 10 10 0.579 10 10 0.912 10 10 0.796 10 10 0.912 10 10 0.971 10 10 0.971 10 10 0.796 10 10 0.971 10 10 0.684 10 10 0.579 10 10 0.218 10 10 0.218 10 10 0.063 10 10 0.052 10 10 0.043 group 6 vs 7 nn p 10 10 0.481 10 10 0.912 10 10 0.015
10 10 0.001
10 10 0.002 10 10 0.009 10 10 0.015 10 10 0.019 10 10 0.190 10 10 0.105 10 10 0.247 10 10 0.631 10 10 0.853 10 10 0.436 10 10 0.218
p<0.05
Page A6 of A172
Appendix to TNO-report of 3M-02
B2. Food intake measurements
Final version
04-12-2006
cage#
84 85 86 87 88 89 90/91 92 93 94 95 96 97 98 99 100 101 102 103
number of mice
3 3 4 5 3 4 4 4 3 4 3 5 2 5 6 4 4 4 5
Food on cage week -2 (g)
86.1 88.8 117.2 131.5 84.2 105.6 108.5 107.7 79.3 104.1 82.6 132.8 54.5 139.5 156.4 105.8 102.2 102.3 132.2
Food off cage week -1 (g)
37.1 41.6 49.1 51.8 22.9 29.3 40.9 49.6 21 40 25.7 40 20.8 57.2 78.2 30.5 40.3 44.7 53.6
days
6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6
Food intake (g/day/mouse)
2.72 2.62 2.84 2.66 3.41 3.18 2.82 2.42 3.24 2.67 3.16 3.09 2.81 2.74 2.17 3.14 2.58 2.40 2.62
2.80 0.32
Food on cage week -1 (g)
82 81.8 108.9 132.7 77.6 103.9 105.2 108.2 80.3 108.8 81.6 130.8 52.2 129.7 152.1 104.2 106.9 106.8 130.6
Food off cage week 0 (g)
25 19.7 30.3 39.4 10.4 20.9 27.7 40.3 16.7 31.1 17.1 28.5 9.5 33.2 51.8 20.5 31 33.2 41.7
days
7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7
Food intake (g/day/mouse)
2.71 2.96 2.81 2.67 3.20 2.96 2.77 2.43 3.03 2.78 3.07 2.92 3.05 2.76 2.39 2.99 2.71 2.63 2.54
2.81 0.22
Page A7 of A172
Page A8 of A172
Group
1 1 1 1 1 AVERAGE SD 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 AVERAGE SD
cage#
1 2 3 4 5
number of mice
2 2 2 1 3
Food on cage week 0 (g)
70.8 69 62.5 31 86.1
Food off cage week 1 (g)
34.8 26.5 26.4 12.5 38.3
days
6 6 6 6 6
6
3
85.8
34.1
6
7
1
38.3
15.3
6
8
3
89.4
31.4
6
9
2
59.8
28.6
6
10
1
34.1
18.2
6
11
1
34.4
17
6
12
1
35
16.4
6
13
2
63
24.3
6
14
1
32.2
13.5
6
15
3
84.1
31.3
6
16
1
29
12.6
6
17
1
30.9
12.2
6
18
2
19
4
20
2
21
2
62.4 110.6 54.5 65.1
20.9
6
47.6
6
12.5
6
29.8
6
22
4
23
1
24
1
25
2
26
1
27
1
108.8 29.5 29.6 53.8 32 32
48.9
6
10.7
6
11.6
6
17.5
6
15.9
6
14.9
6
28
1
29
2
30
2
31
4
32
1
36.1 59.2 60.2 108.1 40.5
16
6
20.1
6
20.7
6
44.7
6
23.6
6
33
1
33.3
14.2
6
34
1
33.8
14.8
6
35
1
40.8
23.1
6
36
2
56.5
16.1
6
37
1
30.8
14.5
6
38
2
50.5
18.1
6
39
2
55.7
25.1
6
Food intake g/day/mouse
3.00 3.54 3.01 3.08 2.66 3.06 0.32 2.87 3.83 3.22 2.60 2.65 3.04 0.51 2.90 3.10 3.23 3.12 2.93 2.73 3.12
3.02 0.17 3.46 2.63 3.50 2.94
3.13 0.42 2.50 3.13 3.00 3.03 2.68 2.85
2.86 0.24
3.35 3.26 3.29 2.64 2.82
3.07 0.32
3.18 3.17 2.95 3.37 2.72 2.70 2.55 2.95 0.30
Food on cage week 1 (g)
51.4 52.3 51.1 29.7 83.6
Food off cage week 2 (g)
12 9.9 10.9 10.5 31.4
80.7
24.4
28.5
4.3
81.3
20.1
52.9
18
31.1
12.1
26.4
6.1
31.4
11.6
60
16.2
27.6
7.8
76.3
23.8
27.9
8.2
28.3
8.5
50.9
11.4
98.6
33.7
48.6
8.1
56.8
17.8
100.7
40.6
24.9
5.6
27.2
11.2
49.2
14.4
25.6
9
29.5
13
29
8.6
53.8
11.8
51.2
9.3
101.5
33
31.1
11.1
30.5
12.8
27.6
11.9
28.2
10.8
55.6
21
29.2
13.5
54.2
25.7
51.2
21.4
days
6 6 6 6 6
6 6 6 6 6
6 6 6 6 6 6 6
6 6 6 6
6 6 6 6 6 6
6 6 6 6 6
6 6 6 6 6 6 6
Food intake g/day/mouse
3.28 3.53 3.35 3.20 2.90 3.25 0.23 3.13 4.03 3.40 2.91 3.17 3.33 0.43 3.38 3.30 3.65 3.30 2.92 3.28 3.30
3.30 0.21 3.29 2.70 3.38 3.25
3.16 0.31 2.50 3.22 2.67 2.90 2.77 2.75
2.80 0.24
3.40 3.50 3.49 2.85 3.33
3.32 0.27
2.95 2.62 2.90 2.88 2.62 2.38 2.48 2.69 0.22
Food on cage week 2 (g)
57.2 55 55.7 27.6 75.7
Food off cage week 2.3
days
77.6 27 83.1 55.5 31.8
29.9 29.4 56.3 30.5 81.6 35.1 30.1
57.4 106.4 53.4 52.3
108.7 31.7 31.3 57.8 30.3 34.1
83.2
2.8
23.7
2.8
24.3
2.8
41.4
2.8
23
2.8
27.4
2.8
35.1 57.4 50.2 104.6 36
37.6
30.8
2.8
32.4
24.6
2.8
31.1
24.8
2.8
54.6
41
2.8
33.9
28.1
2.8
55.7
44.7
2.8
56.4
44.5
2.8
Food intake g/day/mouse
2.28 2.86 2.50 2.93 2.61 2.39 2.59 0.26
2.43 2.79 2.25 2.43 2.07 1.96 2.13 2.29 0.28
Food on cage week 2.3
Food off cage week 3 (g)
15.2 9.3 19.2 5.1 17.4
8.7 9.6 16.5 17.3 10.2
17.5 7.9 8.4 10.3 25.2 15.3 10.1
15.7 24.9 10 11.6
108.6
62.5
27.2
14.9
31.8
20.1
57.1
31.1
29.5
15.5
31.0
17.8
14.3 10.3 7.1 25.2 17.8
32.9
23
33.6
24.2
27.6
16.6
52.5
26.7
28.7
18.5
54.9
34.5
60.0
26.7
days
7 7 7 7 7
7 7 7 7 7
7 7 7 7 7 7 7
7 7 7 7
4.2 4.2 4.2 4.2 4.2 4.2
7 7 7 7 7
4.2 4.2 4.2 4.2 4.2 4.2 4.2
Food intake g/day/mouse
3.00 3.26 2.61 3.21 2.78 2.97 0.28 3.28 2.49 3.17 2.73 3.09 2.95 0.33 1.77 3.07 3.42 2.89 2.69 2.83 2.86
2.94 0.28 2.98 2.91 3.10 2.91
2.97 0.09 2.74 2.93 2.79 3.10 3.33 3.14
3.00 0.23
2.97 3.36 3.08 2.84 2.60
2.97 0.28
2.36 2.24 2.62 3.07 2.43 2.43 3.96 2.73 0.61
Food on cage week 3 (g)
64.6 59.6 59.6 30.6 80.4
Food off cage week 4 (g)
28.8 21 21.2 10.7 30.8
days
6 6 6 6 6
77.4
19.5
6
30.1
8.4
6
79
18.5
6
54.3
19.6
6
33.5
14
6
31.3
11
6
32.8
13.7
6
67.6
24.8
6
27.4
9.1
6
78.4
26.5
6
32.9
14
6
30.2
11.5
6
56.7 110.8 55.6 59.1
18.7
6
46.9
6
16.2
6
23.1
6
104.1 38.5 40.3 65.1 35.3 31.3
30.4
6
22.5
6
19.7
6
21.8
6
18.8
6
12.3
6
29 54.5 54.8 105.1 29.5
9.5
6
16.3
6
19.1
6
39
6
11.1
6
50.1
32.1
6
41.5
23.3
6
31.5
13.4
6
60.4
28.1
6
27.3
8.2
6
52.7
18.4
6
58.5
28.1
6
Food intake g/day/mouse
2.98 3.22 3.20 3.32 2.76 3.09 0.23 3.22 3.62 3.36 2.89 3.25 3.27 0.26 3.38 3.18 3.57 3.05 2.88 3.15 3.12
3.15 0.25 3.17 2.66 3.28 3.00
3.03 0.27 3.07 2.67 3.43 3.61 2.75 3.17
3.12 0.37
3.25 3.18 2.98 2.75 3.07
3.05 0.19
3.00 3.03 3.02 2.69 3.18 2.86 2.53 2.90 0.22
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group cage#
1
1
1
2
1
3
1
4
1
5
AVERAGE
SD
2
6
2
7
2
8
2
9
2
10
AVERAGE
SD
3
11
3
12
3
13
3
14
3
15
3
16
3
17
AVERAGE
SD
4
18
4
19
4
20
4
21
AVERAGE
SD
5
22
5
23
5
24
5
25
5
26
5
27
AVERAGE
SD
6
28
6
29
6
30
6
31
6
32
AVERAGE
SD
7
33
7
34
7
35
7
36
7
37
7
38
7
39
AVERAGE
SD
number of mice 2 2 2 1 3
3 1 3 2 1
1 1 2 1 3 1 1
2 4 2 2
4 1 1 2 1 1
1 2 2 4 1
1 1 1 2 1 2 2
Food on cage week 4 (g)
50.3 55.6 51.7 26.2 83.0
Food off cage week 5 (g)
19.0 22.0 18.3 10.0 34.6
days
5.5 5.5 5.5 5.5 5.5
74.4
26.0
5.5
29.7
10.8
5.5
75.5
24.6
5.5
50.2
23.8
5.5
25.7
9.5
5.5
26.5
9.8
5.5
29.5
14.7
5.5
55.8
18.2
5.5
30.2
16.1
5.5
84.8
42.1
5.5
25.7
10.0
5.5
35.6
20.9
5.5
53.0 107.9 58.2 58.0
19.4
5.5
52.0
5.5
26.7
5.5
29.5
5.5
105.0 30.5 30.2 53.0 28.5 27.6
60.2
4.5
16.8
4.5
15.7
4.5
22.2
4.5
15.4
4.5
14.3
4.5
30.2 50.5 54.1 102.0 30.0
16.3
5.5
18.2
5.5
22.6
5.5
45.9
5.5
17.1
5.5
33.2
17.4
4.5
31.4
16.7
4.5
41.8
29.1
4.5
45.3
16.2
4.5
26.2
13.8
4.5
65.5
35.9
4.5
55.0
33.8
4.5
Food intake g/day/mouse
2.85 3.05 3.04 2.95 2.93 2.96 0.08 2.93 3.44 3.08 2.40 2.95 2.96 0.37 3.04 2.69 3.42 2.56 2.59 2.85 2.67 2.83 0.31 3.05 2.54 2.86 2.59 2.76 0.24 2.49 3.04 3.22 3.42 2.91 2.96 3.01 0.32 2.53 2.94 2.86 2.55 2.34 2.64 0.25 3.51 3.27 2.82 3.23 2.76 3.29 2.36 3.03 0.40
Food on cage week 5 (g)
60.2 60.7 57.2 31.6 77.1
Food off cage week 6 (g)
22.1 19.5 16.8 10.7 19.2
85.4
19.8
37.4
12.4
85.2
14.2
51.9
13.6
33.6
13.4
36
15.7
37.4
16.2
55.9
7.1
35.4
15.6
89.8
32.7
27.4
8
31.2
9.4
57.7
16.5
106.9
33.2
53.4
11.1
57.7
17.7
109.2
32.3
41.4
20.1
32.5
10.6
61.1
15.5
32.8
12.5
31.6
10.2
36.3
16.6
60.8
20.3
52.6
12.3
105.5
36.7
34.8
16
33.2
12.7
35.4
16.3
39.9
20
78.1
38.8
31.1
12.1
61.2
24.9
52.9
15
days
7 7 7 7 7
7 7 7 7 7
7 7 7 7 7 7 7
7 7 7 7
7 7 7 7 7 7
7 7 7 7 7
7 7 7 7 7 7 7
Food intake g/day/mouse
2.72 2.94 2.89 2.99 2.76 2.86 0.12 3.12 3.57 3.38 2.74 2.89 3.14 0.34 2.90 3.03 3.49 2.83 2.72 2.77 3.11 2.98 0.26 2.94 2.63 3.02 2.86 2.86 0.17 2.75 3.04 3.13 3.26 2.90 3.06 3.02 0.18 2.81 2.89 2.88 2.46 2.69 2.75 0.18 2.93 2.73 2.84 2.81 2.71 2.59 2.71 2.76 0.11
Food on cage week 6 (g)
78.3 73.4 67 38.3 94.8
Food off cage week 7 (g)
61.7 55.5 48.9 29.1 71
days
3 3 3 3 3
97.1
69.2
3
42
32.6
3
85.6
54.1
3
60.9
46.3
3
46.3
37.3
3
39.9 40.9 56.5 46.1 114.5 34.9 37.9
30.3
3
31.4
3
39.2
3
37.8
3
91.4
3
26.2
3
29.5
3
61.5 130.6
70 67.9
42.6
3
98.9
3
50.3
3
50.3
3
138.4 52.3 37.3 74.9 39.9 35.4
107.2
3
44.3
3
27.6
3
53.8
3
31.1
3
26.8
3
44.3
34.9
3
66.8
48.7
3
60
42.9
3
142
112.9
3
46.7
39
3
35.7
27.4
3
42.4
33.8
3
46.1
36.9
3
90.6
73.3
3
39.7
30.7
3
76.5
60.4
3
65.9
50.8
3
Food intake g/day/mouse
2.77 2.98 3.02 3.07 2.64 2.90 0.18 3.10 3.13 3.50 2.43 3.00 3.03 0.39 3.20 3.17 2.88 2.77 2.57 2.90 2.80 2.90 0.22 3.15 2.64 3.28 2.93 3.00 0.28 2.60 2.67 3.23 3.52 2.93 2.87 2.97 0.35 3.13 3.02 2.85 2.43 2.57 2.80 0.30 2.77 2.87 3.07 2.88 3.00 2.68 2.52 2.83 0.19
Page A9 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group cage# mouse# Food on cage Food off cage days Food intake Food on cage
week 7 (g) week 7.5 (g)
g/day/mouse week 7.5(g)
1
1
1
438.1
428.4
3
3.22
29.8
1
MC 101
2
metabolic cage
20.3
1
MC 105
3
metabolic cage
18.6
1
MC 201
4
metabolic cage
23.5
1
3
5
485.4
476.5
3
2.96
26.1
1
MC 206
6
metabolic cage
19.2
1
4
7
496.1
3
2.86
29.2
1
5
8,9,10
425.4
397.3
3
3.12
54.7
AVERAGE
3.04
SD
0.16
2
6
11,12,13
435
407.6
3
3.04
55.9
2
7
14
429.7
421.0
3
2.91
21.9
2
8
15,16,17
475.5
442.1
3
3.71
44.3
2
9
18,19
450.2
431.3
3
3.15
33.6
2
10
20
425.7
9.5
3
2.94
32.1
AVERAGE
3.15
SD
0.33
3
MC 102
21
metabolic cage
35.6
3
MC 106
22
metabolic cage
29.4
3
13
23
455.8
445.8
3
3.35
33.1
3
MC 202
24
metabolic cage
26.8
3
14
25
430.2
418.8
3
3.79
36.9
3
15
26,28
465.3
449.8
3
2.58
45.1
3
MC 206
27
metabolic cage
32.9
3
16
29
437.8
428.5
3
3.10
26.3
3
17
30
429.1
421.5
3
2.53
44.1
AVERAGE
3.07
SD
0.53
4
MC 103
31
metabolic cage
44.3
4
MC 107
32
metabolic cage
51.2
4
19 33,34,35,36 486.1
448.3
3
3.15
84.3
4
MC 203
37
metabolic cage
33.1
4
MC207
38
metabolic cage
32.6
4
21
39,40
436.2
419.7
3
2.75
44.2
AVERAGE
2.95
SD
0.28
5
22 41,42,43,44 505.9
470.3
3
2.97
109.2
5
23
45
443.9
434.1
3
3.26
41.4
5
24
46
438.7
428.7
3
3.35
32.5
5
25
47,48
443.3
423.9
3
3.24
61.1
5
26
49
428.6
419.7
3
2.98
32.8
5
27
50
436.1
427.5
3
2.86
31.6
AVERAGE
3.11
SD
0.20
6
MC104
51
metabolic cage
38.2
6
29
52
432.6
424.2
3
2.81
27.6
6
MC 108
53
metabolic cage
33.1
6
MC204
54
metabolic cage
28.4
6
MC208
55
metabolic cage
33.1
6
31 56,57,58,59 409.8
371.4
3
3.20
87.4
6
32
60
441.6
433.5
3
2.69
21.7
AVERAGE
2.90
SD
0.27
7
33
61
439.2
429.8
3
3.12
33.1
7
34
62
431.4
422.0
3
3.15
32.5
7
35
63
442.7
434.7
3
2.67
39.4
7
36
64,65
445.6
427.0
3
3.10
66.1
7
37
66
426.1
417.7
3
2.79
30.2
7
38
67,68
465.5
446.0
3
3.25
65.8
7
39
69,70
445.2
423.9
3
3.55
52.1
AVERAGE
3.09
SD
0.29
NB: week 7-7.5: all mice are put on powdered food because of metabolic cages of part of mice
Food off cage week 8 (g) 15.7 12.8 8.7 15.0 13.9 8.3 18.2 25.8
12.5 11.9 11.7 8.4 17.3
26.2 21.4 20.3 17.7 24.7 20.5 25.1 15.5 34.7
35.6 43.3 36.0 23.1 23.2 27.0
63.4 31.1 18.9 38.2 20.9 22.2
27.5 15.6 25.6 20.7 24.7 46.3 11.4
22.4 22.5 27.5 39.3 18.8 40.7 32.5
days
4 3 3 3 4 3 4 4
4 4 4 4 4
3 3 4 3 4 4 3 4 4
3 3 4 3 3 4
4 4 4 4 4 4
3 4 3 3 3 4 4
4 4 4 4 4 4 4
Food intake g/day/mouse
3.52 2.51 3.29 2.84 3.05 3.63 2.75 2.41
3.00 0.45
3.62 2.51 2.72 3.15 3.70
3.14 0.53
3.12 2.67 3.20 3.02 3.06 3.08 2.61 2.71 2.36
2.87 0.29
2.89 2.62 3.02 3.34 3.14 2.15
2.86 0.42
2.86 2.57 3.41 2.86 2.98 2.36
2.84 0.36
3.58 3.00 2.49 2.57 2.81 2.57 2.58
2.80 0.39
2.68 2.51 2.98 3.35 2.84 3.14 2.45
2.85 0.33
Page A10 of A172
Appendix to TNO-report of 3M-02
Final version
Group Mouse#
1
1
1
2
1
3
1
4
1
5
1
6
1
7
1
8
1
9
1
10
AVERAGE
SD
2
11
2
12
2
13
2
14
2
15
2
16
2
17
2
18
2
19
2
20
AVERAGE
SD
3
21
3
22
3
23
3
24
3
25
3
26
3
27
3
28
3
29
3
30
AVERAGE
SD
4
31
4
32
4
33
4
34
4
35
4
36
4
37
4
38
4
39
4
40
AVERAGE
SD
5
41
5
42
5
43
5
44
5
45
5
46
5
47
5
48
5
49
5
50
AVERAGE
SD
6
51
6
52
6
53
6
54
6
55
6
56
6
57
6
58
6
59
6
60
AVERAGE
SD
7
61
7
62
7
63
7
64
7
65
7
66
7
67
7
68
7
69
7
70
AVERAGE
SD
cage#
1 1 2 2 3 3 4 5 5 5
6 6 6 7 8 8 8 9 9 10
11 12 13 13 14 15 15 15 16 17
18 18 19 19 19 19 20 20 21 21
22 22 22 22 23 24 25 25 26 27
28 29 29 30 30 31 31 31 31 32
33 34 35 36 36 37 38 38 39 39
Food on cage week 8 (g)
34.3 27.1 28.6 44.1 36.9 43.3 42.2 34.2 33.0 32.9
Food off cage week 9 (g)
11.7 5.8 7.9 24.6 15.6 20.5 22.2 10.1 12.1 8.6
days
7 7 7 7 7 7 7 7 7 7
34.1
9.5
7
36.5
16.4
7
28.4
6.3
7
29.5
11.4
7
28.3
5.8
7
27.1
3.2
7
36.0
15.9
7
44.3
22.2
7
45.2
25.0
7
33.9
9.3
7
34.0
12.0
7
35.1
14.2
7
29.8
7.7
7
27.6
8.2
7
44.1
18.8
7
44.3
23.4
7
47.2
26.3
7
34.9
13.8
7
33.2
13.1
7
32.6
12.6
7
33.1
11.5
7
34.2
15.5
7
45.7
24.8
7
36.0
14.0
7
45.7
23.0
7
44.1
25.6
7
36.9
17.2
7
32.1
10.7
7
22.5
1.2
7
33.4
12.3
7
44.1
19.5
7
37.7
18.9
7
45.1
21.2
7
44.2
25.6
7
35.6
15.2
7
43.2
19.8
7
44.8
26.1
7
42.5
18.6
7
29.6
7.9
7
34.3
15.6
7
33.9
10.7
7
32.4
14.6
7
35.6
18.6
7
41.8
19.9
7
42.1
22.0
7
44.0
23.1
7
43.3
23.1
7
32.5
9.8
7
28.2
7.1
7
29.4
12.0
7
34.4
14.3
7
45.2
21.3
7
30.1
8.8
7
29.8
7.7
7
27.2
3.8
7
33.4
12.5
7
31.5
10.2
7
46.2
26.1
7
44.1
23.2
7
32.5
14.4
7
Food intake g/day/mouse
3.23 3.05 2.96 2.79 3.05 3.26 2.86 3.45 2.98 3.47
3.11 0.23
3.52 2.87 3.16 2.58 3.22 3.42 2.87 3.16 2.88 3.52
3.12 0.32
3.15 2.98 3.16 2.77 3.62 2.99 2.99 3.01 2.87 2.86
3.04 0.24
3.08 2.67 2.98 3.15 3.24 2.65 2.81 3.06 3.04 3.02
2.97 0.20
3.52 2.68 3.42 2.66 2.91 3.35 2.67 3.42 3.10 2.67
3.04 0.36
3.32 2.55 2.43 3.13 2.87 2.98 2.88 3.24 3.01 2.49
2.89 0.31
2.87 3.41 3.05 3.16 3.34 2.99 3.04 2.87 2.99 2.58
3.03 0.24
04-12-2006
Page A11 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0-1 1-2 2-3 (gr 5,7: 2.3-3) 3-4 4-5 5-6 6-7 7-7.5 7.5-8 8-9
week
0-1 1-2 2-3 (gr 5,7: 2.3-3) 3-4 4-5 5-6 6-7 7-7.5 7.5-8 8-9
week
0-1 1-2 2-2.3 2-3 (gr 5,7: 2.3-3) 3-4 4-5 5-6 6-7 7-7.5 7.5-8 8-9
group 1 vs 2 nn p 5 5 0.690 5 5 1.000 5 5 1.000 5 5 0.222 5 5 0.841 5 5 0.222 5 5 0.310 4 5 0.905 8 5 0.622 10 10 0.912 group 2 vs 4 nn p 5 4 0.730 5 4 0.905 5 4 0.905 5 4 0.286 5 4 0.413 5 4 0.286 5 4 1.000 5 2 0.571 5 6 0.429 10 10 0.247 group 3 vs 7 nn p 7 7 0.805 7 7 0.001
group 1 vs 3 nn p 5 7 0.876 5 7 0.530 5 7 0.755 5 7 0.876 5 7 0.202 5 7 0.432 5 7 1.000 4 5 1.000 8 9 0.606 10 10 0.529 group 2 vs 5 nn p 5 6 0.792 5 6 0.030 5 6 0.792 5 6 0.429 5 6 0.931 5 6 0.792 5 6 0.792 5 6 0.931 5 6 0.429 10 10 0.631 group 4 vs 5 nn p 4 6 0.476 4 6 0.114
Food intake, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
nn p nn p nn
p
5 4 0.905 5 6 0.429 5 5 1.000
5 4 0.905 5 6 0.017 5 5 0.690
5 4 0.905 5 6 0.931 5 5 1.000
5 4 0.730 5 6 0.931 5 5 0.548
5 4 0.286 5 6 0.662 5 5 0.056
5 4 0.222 5 6 0.126 5 5 0.222
5 4 0.310 5 6 1.000 5 5 0.690
4 2 0.800 4 6 0.352 4 3 0.400
8 6 0.755 8 6 0.662 8 7 0.463
10 10 0.353 10 10 0.579 10 10 0.190
group 2 vs 6 group 2 vs 7
group 3 vs 4
nn p nn p nn
p
5 5 0.690 5 7 1.000 7 4 0.648
5 5 0.690 5 7 0.005 7 4 0.315
5 5 1.000 5 7 0.149 7 4 0.230
5 5 0.151 5 7 0.030 7 4 0.527
5 5 0.151 5 7 0.876 7 4 0.788
5 5 0.056 5 7 0.030 7 4 0.648
5 5 0.421 5 7 0.149 7 4 0.527
5 3 0.393 5 7 1.000 5 2 1.000
5 7 0.268 5 7 0.268 9 6 0.955
10 10 0.165 10 10 0.436 10 10 0.971
group 4 vs 6 group 4 vs 7
group 5 vs 6
nn p nn p nn
p
4 5 0.730 4 7 0.527 6 5 0.329
4 5 0.190 4 7 0.042 6 5 0.017
7 7 0.383 4 6 0.762 4 5 0.905 4 7 0.230 6 5 7 7 0.026 4 6 0.610 4 5 1.000 4 7 0.527 6 5 7 7 0.318 4 6 0.352 4 5 0.413 4 7 0.315 6 5 7 7 0.073 4 6 0.171 4 5 0.413 4 7 0.230 6 5 7 7 0.535 4 6 0.762 4 5 0.286 4 7 0.315 6 5 5 7 0.755 2 6 0.429 2 3 1.000 2 7 0.667 6 3 9 7 0.918 6 6 0.699 6 7 0.445 6 7 0.836 6 7 10 10 0.684 10 10 0.631 10 10 0.529 10 10 0.739 10 10
0.931 0.931 0.082 0.030 0.429 0.167 0.836 0.315
group 1 vs 7 nn p 5 7 0.755 5 7 0.005 5 7 0.202 5 7 0.202 5 7 0.755 5 7 0.149 5 7 0.639 4 7 0.788 8 7 0.613 10 10 0.631 group 3 vs 5 nn p 7 6 0.295 7 6 0.002 7 6 0.445 7 6 0.836 7 6 0.366 7 6 0.445 7 6 0.731 5 6 0.931 9 6 0.607 10 10 0.796 group 5 vs 7 nn p 6 7 0.534 6 7 0.445 6 7 0.073 6 7 0.101 6 7 0.295 6 7 0.836 6 7 0.014 6 7 0.628 6 7 0.836 6 7 0.945 10 10 0.853
group 2 vs 3 nn p 5 7 0.639 5 7 0.755 5 7 0.639 5 7 0.432 5 7 0.432 5 7 0.432 5 7 0.530 5 5 1.000 5 9 0.298 10 10 0.436 group 3 vs 6 nn p 7 5 0.530 7 5 0.432 7 5 0.639 7 5 0.432 7 5 0.268 7 5 0.149 7 5 0.530 5 3 1.000 9 7 0.299 10 10 0.481 group 6 vs 7 nn p 5 7 0.530 5 7 0.018
5 7 0.202 5 7 0.268 5 7 0.149 5 7 1.000 5 7 1.000 3 7 0.667 7 7 0.805 10 10 0.315
p<0.05
Page A12 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Appendix C. Plasma measurements
C1. Plasma cholesterol measurements
group Mouse# cage#
1
1
1
1
2
1
1
3
2
1
4
2
1
5
3
1
6
3
1
7
4
1
8
5
1
9
5
1
10
5
AVERAGE
SD
2
11
6
2
12
6
2
13
6
2
14
7
2
15
8
2
16
8
2
17
8
2
18
9
2
19
9
2
20
10
AVERAGE
SD
3
21
11
3
22
12
3
23
13
3
24
13
3
25
14
3
26
15
3
27
15
3
28
15
3
29
16
3
30
17
AVERAGE
SD
4
31
18
4
32
18
4
33
19
4
34
19
4
35
19
4
36
19
4
37
20
4
38
20
4
39
21
4
40
21
AVERAGE
SD
5
41
22
5
42
22
5
43
22
5
44
22
5
45
23
5
46
24
5
47
25
5
48
25
5
49
26
5
50
27
AVERAGE
SD
6
51
28
6
52
29
6
53
29
6
54
30
6
55
30
6
56
31
6
57
31
6
58
31
6
59
31
6
60
32
AVERAGE
SD
7
61
33
7
62
34
7
63
35
7
64
36
7
65
36
7
66
37
7
67
38
7
68
38
7
69
39
7
70
39
AVERAGE
SD
excluded 655 85
excluded 656 86
excluded 741 96
excluded 742 96
excluded 748 97
eartag LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
12 00 21 02 20
Measured cholesterol (mmol/l) week 0 week 1 week 4 week 8
3.219 2.814 2.172 2.211 3.446 3.088 2.599 2.394 3.633 3.176 2.120 1.725 3.260 2.556 1.911 1.877 1.847 2.612 2.042 1.733 3.819 3.458 2.033 2.500 4.055 3.958 1.815 2.166 3.949 3.668 2.347 2.295 4.590 3.257 1.972 1.816 3.706 3.015 1.911 1.854
3.998 1.791 4.428 4.274 4.453 1.774 1.750 4.079 4.055 3.633
3.901 2.145 3.281 2.878 3.080 2.306 2.322 3.450 2.959 3.233
2.625 1.110 2.094 1.859 2.443 1.057 1.563 2.233 2.233 2.085
2.181 0.867 2.287 1.353 2.272 0.837 1.209 2.151 2.439 2.029
4.201 3.446 4.047 4.071 3.649 3.893 3.130 3.519 4.290 2.083
3.217 3.007 3.257 2.999 2.685 2.741 2.798 3.201 3.466 1.968
1.850 1.876 2.077 1.920 1.406 1.885 1.737 1.850 2.521 1.153
2.439 2.021 1.771 2.318 1.763 1.870 1.703 2.219 2.431 1.315
3.974 3.625 4.104 3.122 3.698 1.701 3.576 3.511 3.917 2.010
1.565 1.622 2.057 1.912 2.322 1.904 1.895 1.879 2.467 1.557
1.327 1.327 1.476 1.284 1.380 1.301 1.467 1.354 1.754 1.293
1.125 1.353 1.422 1.186 1.490 1.209 1.452 1.156 1.672 1.429
1.661 3.617 3.682 3.795 4.323 3.901 3.641 3.065 1.669 2.708
1.871 1.525 1.839 1.670 3.056 2.226 1.485 1.638 2.008 2.274
1.179 1.197 1.162 1.188 1.258 1.388 0.813 1.449 1.354 1.467
1.072 1.885 1.983 1.490 2.591 2.454 0.746 1.429 1.247 2.097
2.862 3.138 4.086 3.316 3.511 3.584 1.523 3.795 3.357 3.365
1.992 2.604 2.556 2.258 2.492 2.508 1.654 2.330 2.024 2.065
1.101 1.101 1.223 1.110 1.136 1.110 1.214 1.005 1.249 1.310
1.794 1.247 1.953 1.611 1.543 1.968 0.928 1.452 1.763 2.386
3.462 3.763 3.024 3.479 3.324 3.357 4.241 4.274 3.917 3.795
1.646 1.775 1.541 1.549 1.501 1.581 1.879 1.928 1.839 1.783
2.338 3.174 2.573 2.242 1.920 2.077 1.998 1.963 1.824 1.894
2.113 2.021 2.447 2.280 1.816 1.832 1.733 2.158 1.695 1.505
6.230 1.336 1.360 0.792 0.776
week 0 2 2 2 2 2 2 2 2 2 2
2 2 2 2 2 2 2 2 2 2
2 2 2 2 2 2 2 2 2 2
2 2 2 2 2 2 2 2 2 2
2 2 2 2 2 2 2 2 2 2
2 2 2 2 2 2 2 2 2 2
2 2 2 2 2 2 2 2 2 2
2 2 2 2 2
dilution
week 1 week 4
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
week 8 3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
week 0
6.438 6.892 7.266 6.520 3.694 7.638 8.110 7.898 9.180 7.412
7.10 1.45
7.996 3.582 8.856 8.548 8.906 3.548 3.500 8.158 8.110 7.266
6.85 2.33
8.402 6.892 8.094 8.142 7.298 7.786 6.260 7.038 8.580 4.166
7.27 1.31
7.948 7.250 8.208 6.244 7.396 3.402 7.152 7.022 7.834 4.020
6.65 1.65
3.322 7.234 7.364 7.590 8.646 7.802 7.282 6.130 3.338 5.416
6.41 1.85
5.724 6.276 8.172 6.632 7.022 7.168 3.046 7.590 6.714 6.730
6.51 1.39
6.924 7.526 6.048 6.958 6.648 6.714 8.482 8.548 7.834 7.590
7.33 0.81
12.460 2.672 2.720 1.584 1.552
Cholesterol (mmol/l)
week 1 week 4
5.628
6.516
6.176
7.797
6.352
6.360
5.112
5.733
5.224
6.126
6.916
6.099
7.916
5.445
7.336
7.041
6.514
5.916
6.030
5.733
6.32
6.28
0.90
0.70
7.802
7.875
4.290
3.330
6.562
6.282
5.756
5.577
6.160
7.329
4.612
3.171
4.644
4.689
6.900
6.699
5.918
6.699
6.466
6.255
5.91
5.79
1.12
1.60
6.434
5.550
6.014
5.628
6.514
6.231
5.998
5.760
5.370
4.218
5.482
5.655
5.596
5.211
6.402
5.550
6.932
7.563
3.936
3.459
5.87
5.48
0.84
1.10
3.130
3.981
3.244
3.981
4.114
4.428
3.824
3.852
4.644
4.140
3.808
3.903
3.790
4.401
3.758
4.062
4.934
5.262
3.114
3.879
3.84
4.19
0.61
0.43
3.742
3.537
3.050
3.591
3.678
3.486
3.340
3.564
6.112
3.774
4.452
4.164
2.970
2.439
3.276
4.347
4.016
4.062
4.548
4.401
3.92
3.74
0.94
0.57
3.984
3.303
5.208
3.303
5.112
3.669
4.516
3.330
4.984
3.408
5.016
3.330
3.308
3.642
4.660
3.015
4.048
3.747
4.130
3.930
4.50
3.47
0.62
0.27
3.292
7.014
3.550
9.522
3.082
7.719
3.098
6.726
3.002
5.760
3.162
6.231
3.758
5.994
3.856
5.889
3.678
5.472
3.566
5.682
3.40
6.60
0.31
1.24
week 8
6.633 7.182 5.175 5.631 5.199 7.500 6.498 6.885 5.448 5.562
6.17 0.87
6.543 2.601 6.861 4.059 6.816 2.511 3.627 6.453 7.317 6.087
5.29 1.88
7.317 6.063 5.313 6.954 5.289 5.610 5.109 6.657 7.293 3.945
5.96 1.10
3.375 4.059 4.266 3.558 4.470 3.627 4.356 3.468 5.016 4.287
4.05 0.53
3.216 5.655 5.949 4.470 7.773 7.362 2.238 4.287 3.741 6.291
5.10 1.81
5.382 3.741 5.859 4.833 4.629 5.904 2.784 4.356 5.289 7.158
4.99 1.23
6.339 6.063 7.341 6.840 5.448 5.496 5.199 6.474 5.085 4.515
5.88 0.88
Page A13 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0 1 4 8
week
0 1 4 8
week
0 1 4 8
group 1 vs 2 nn p 10 10 0.631 10 10 0.436 10 10 0.912 10 10 0.436 group 2 vs 4 nn p 10 10 0.247 10 10 <0.001 10 10 0.029 10 10 0.190 group 3 vs 7 nn p 10 10 0.739 10 10 <0.001 10 10 0.023 10 10 0.796
group 1 vs 3 nn p 10 10 0.631 10 10 0.393 10 10 0.035 10 10 0.796 group 2 vs 5 nn p 10 10 0.218 10 10 <0.001 10 10 0.015 10 10 0.796 group 4 vs 5 nn p 10 10 0.739 10 10 0.739 10 10 0.089 10 10 0.165
Plasma cholesterol, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn p nn p nn
p
nn p
10 10 0.631 10 10 0.393 10 10 0.247 10 10 0.912
10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 <0.001
10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 0.853
10 10 <0.001 10 10 0.247 10 10 0.035 10 10 0.393
group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
nn p nn p nn
p
nn p
10 10 0.190 10 10 0.529 10 10 0.353 10 10 0.315
10 10 0.009 10 10 <0.001 10 10 <0.001 10 10 0.001
10 10 0.007 10 10 0.579 10 10 0.005 10 10 0.002
10 10 0.579 10 10 0.853 10 10 <0.001 10 10 0.393
group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn p nn p nn
p
nn p
10 10 0.393 10 10 0.654 10 10 0.631 10 10 0.436
10 10 0.015 10 10 0.052 10 10 0.052 10 10 0.247
10 10 <0.001 10 10 <0.001 10 10 0.089 10 10 <0.001
10 10 0.023 10 10 <0.001 10 10 0.853 10 10 0.315
group 2 vs 3 nn p 10 10 0.796 10 10 0.853 10 10 0.393 10 10 0.579 group 3 vs 6 nn p 10 10 0.123 10 10 0.001 10 10 <0.001 10 10 0.089 group 6 vs 7 nn p 10 10 0.190 10 10 <0.001 10 10 <0.001 10 10 0.089
p<0.05
Page A14 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
C2. Plasma triglyceride measurements
group Mouse# cage#
1
1
1
1
2
1
1
3
2
1
4
2
1
5
3
1
6
3
1
7
4
1
8
5
1
9
5
1
10
5
AVERAGE
SD
2
11
6
2
12
6
2
13
6
2
14
7
2
15
8
2
16
8
2
17
8
2
18
9
2
19
9
2
20
10
AVERAGE
SD
3
21
11
3
22
12
3
23
13
3
24
13
3
25
14
3
26
15
3
27
15
3
28
15
3
29
16
3
30
17
AVERAGE
SD
4
31
18
4
32
18
4
33
19
4
34
19
4
35
19
4
36
19
4
37
20
4
38
20
4
39
21
4
40
21
AVERAGE
SD
5
41
22
5
42
22
5
43
22
5
44
22
5
45
23
5
46
24
5
47
25
5
48
25
5
49
26
5
50
27
AVERAGE
SD
6
51
28
6
52
29
6
53
29
6
54
30
6
55
30
6
56
31
6
57
31
6
58
31
6
59
31
6
60
32
AVERAGE
SD
7
61
33
7
62
34
7
63
35
7
64
36
7
65
36
7
66
37
7
67
38
7
68
38
7
69
39
7
70
39
AVERAGE
SD
excluded 655 85
excluded 656 86
excluded 741 96
excluded 742 96
excluded 748 97
eartag LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
12 00 21 02 20
Measured TG (mmol/l) week 0 week 1 week 4 week 8
0.445 0.387 0.257 0.298 0.467 0.270 0.263 0.266 0.950 0.731 0.290 0.233 0.706 0.609 0.329 0.277 1.666 0.583 0.214 0.277 0.645 0.472 0.345 0.244 0.595 0.625 0.247 0.471 0.495 0.482 0.208 0.293 0.562 0.477 0.296 0.233 0.756 0.456 0.186 0.212
week 0
2 2 2 2 2 2 2 2 2 2
dilution
week 1 week 4
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
2
3
week 8
3 3 3 3 3 3 3 3 3 3
0.584 0.434 0.274 0.255 2
2
3
3
0.695 0.667 0.356 0.336 2
2
3
3
0.639 0.445 0.219 0.255 2
2
3
3
0.961 0.535 0.219 0.222 2
2
3
3
1.366 0.842 0.340 0.461 2
2
3
3
0.833 0.688 0.422 0.655 2
2
3
3
0.417 1.080 0.515 0.585 2
2
3
3
0.589 0.376 0.257 0.201 2
2
3
3
0.467 0.477 0.252 0.320 2
2
3
3
0.501 0.355 0.214 0.195 2
2
3
3
0.573 0.276 0.121 0.190 2
2
3
3
0.606 0.307 0.148 0.201 2
2
3
3
0.978 0.762 0.307 0.233 2
2
3
3
0.661 0.932 0.372 0.168 2
2
3
3
0.795 0.456 0.143 0.190 2
2
3
3
0.656 0.482 0.148 0.244 2
2
3
3
0.478 0.376 0.143 0.152 2
2
3
3
0.528 0.382 0.137 0.195 2
2
3
3
0.412 0.403 0.208 0.336 2
2
3
3
0.900 0.673 0.312 0.369 2
2
3
3
0.856 0.244 0.137 0.082 2
2
3
3
0.634 0.281 0.143 0.082 2
2
3
3
0.445 0.276 0.121 0.087 2
2
3
3
0.506 0.233 0.099 0.103 2
2
3
3
0.589 0.207 0.132 0.093 2
2
3
3
0.489 0.297 0.230 0.206 2
2
3
3
0.739 0.329 0.132 0.082 2
2
3
3
0.994 0.419 0.148 0.082 2
2
3
3
0.562 0.270 0.104 0.103 2
2
3
3
0.673 0.339 0.186 0.190 2
2
3
3
0.229 0.376 0.121 0.120 2
2
3
3
0.456 0.212 0.132 0.228 2
2
3
3
0.512 0.165 0.099 0.206 2
2
3
3
1.194 0.202 0.132 0.222 2
2
3
3
0.928 0.218 0.099 0.358 2
2
3
3
0.634 0.181 0.110 0.320 2
2
3
3
0.722 0.260 0.247 0.309 2
2
3
3
0.678 0.207 0.208 0.250 2
2
3
3
0.589 0.323 0.870 0.103 2
2
3
3
0.551 0.186 0.115 0.260 2
2
3
3
0.728 0.424 0.110 0.131 2
2
3
3
0.545 0.773 0.170 0.125 2
2
3
3
0.850 0.620 0.208 0.201 2
2
3
3
1.227 0.916 0.159 0.136 2
2
3
3
0.667 0.720 0.170 0.212 2
2
3
3
0.606 0.487 0.159 0.228 2
2
3
3
0.168 0.244 0.154 0.114 2
2
3
3
0.628 0.588 0.154 0.179 2
2
3
3
0.578 0.419 0.154 0.168 2
2
3
3
0.467 0.366 0.126 0.325 2
2
3
3
1.349 0.196 0.263 0.168 2
2
3
3
0.717 0.228 0.361 0.163 2
2
3
3
0.650 0.249 0.356 0.212 2
2
3
3
0.856 0.244 0.203 0.217 2
2
3
3
0.784 0.265 0.236 0.260 2
2
3
3
0.539 0.329 0.318 0.212 2
2
3
3
0.606 0.202 0.312 0.201 2
2
3
3
0.428 0.265 0.225 0.379 2
2
3
3
0.573 0.302 0.230 0.212 2
2
3
3
0.473 0.255 0.132 0.136 2
2
3
3
1.954
2
0.978
2
0.917
2
0.251
2
0.256
2
week 0
0.89 0.934
1.9 1.412 3.332 1.29 1.19 0.99 1.124 1.512
1.46 0.73
1.168 1.39 1.278 1.922 2.732 1.666 0.834 1.178 0.934 1.002
1.41 0.57
1.146 1.212 1.956 1.322 1.59 1.312 0.956 1.056 0.824
1.8
1.32 0.36
1.712 1.268 0.89 1.012 1.178 0.978 1.478 1.988 1.124 1.346
1.30 0.35
0.458 0.912 1.024 2.388 1.856 1.268 1.444 1.356 1.178 1.102
1.30 0.53
1.456 1.09 1.7 2.454 1.334 1.212 0.336 1.256 1.156 0.934
1.29 0.54
2.698 1.434
1.3 1.712 1.568 1.078 1.212 0.856 1.146 0.946
1.40 0.53
3.908 1.956 1.834 0.502 0.512
TG (mmol/l)
week 1 week 4
0.774
0.771
0.54
0.789
1.462
0.87
1.218
0.987
1.166
0.642
0.944
1.035
1.25
0.741
0.964
0.624
0.954
0.888
0.912
0.558
1.02
0.79
0.26
0.16
0.868
0.822
1.334
1.068
0.89
0.657
1.07
0.657
1.684
1.02
1.376
1.266
2.16
1.545
0.752
0.771
0.954
0.756
0.71
0.642
1.18
0.92
0.46
0.30
0.552
0.363
0.614
0.444
1.524
0.921
1.864
1.116
0.912
0.429
0.964
0.444
0.752
0.429
0.764
0.411
0.806
0.624
1.346
0.936
1.01
0.61
0.43
0.27
0.488
0.411
0.562
0.429
0.552
0.363
0.466
0.297
0.414
0.396
0.594
0.69
0.658
0.396
0.838
0.444
0.54
0.312
0.678
0.558
0.58
0.43
0.12
0.12
0.752
0.363
0.424
0.396
0.33
0.297
0.404
0.396
0.436
0.297
0.362
0.33
0.52
0.741
0.414
0.624
0.646
2.61
0.372
0.345
0.47
0.42
0.14
0.16
0.848
0.33
1.546
0.51
1.24
0.624
1.832
0.477
1.44
0.51
0.974
0.477
0.488
0.462
1.176
0.462
0.838
0.462
0.732
0.378
1.11
0.47
0.41
0.08
0.392
0.789
0.456
1.083
0.498
1.068
0.488
0.609
0.53
0.708
0.658
0.954
0.404
0.936
0.53
0.675
0.604
0.69
0.51
0.396
0.51
0.79
0.08
0.22
week 8
0.894 0.798 0.699 0.831 0.831 0.732 1.413 0.879 0.699 0.636
0.84 0.22
0.765 1.008 0.765 0.666 1.383 1.965 1.755 0.603 0.96 0.585
1.05 0.49
0.57 0.603 0.699 0.504 0.57 0.732 0.456 0.585 1.008 1.107
0.68 0.21
0.246 0.246 0.261 0.309 0.279 0.618 0.246 0.246 0.309 0.57
0.33 0.14
0.36 0.684 0.618 0.666 1.074 0.96 0.927 0.75 0.309 0.78
0.76 0.21
0.393 0.375 0.603 0.408 0.636 0.684 0.342 0.537 0.504 0.975
0.55 0.19
0.504 0.489 0.636 0.651 0.78 0.636 0.603 1.137 0.636 0.408
0.65 0.20
Page A15 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week
0 1 4 8
week
0 1 4 8
week
0 1 4 8
group 1 vs 2 nn p 10 10 0.971 10 10 0.739 10 10 0.393 10 10 0.684 group 2 vs 4 nn p 10 10 0.912 10 10 <0.001 10 10 <0.001 10 10 <0.001 group 3 vs 7 nn p 10 10 1.000 10 10 <0.001 10 10 0.123 10 10 1.000
group 1 vs 3 nn p 10 10 0.971 10 10 0.631 10 10 0.089 10 10 0.043 group 2 vs 5 nn p 10 10 0.796 10 10 <0.001 10 9 <0.001 10 10 0.218 group 4 vs 5 nn p 10 10 1.000 10 10 0.029 10 9 0.400 10 10 <0.001
Plasma triglycerides, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn p nn p nn
p
nn p
10 10 0.912 10 10 0.796 10 10 0.912 10 10 0.912
10 10 <0.001 10 10 <0.001 10 10 0.739 10 10 <0.001
10 10 <0.001 10 9 <0.001 10 10 <0.001 10 10 0.912
10 10 <0.001 10 10 0.353 10 10 0.002 10 10 0.007
group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
nn p nn p nn
p
nn p
10 10 0.853 10 10 0.971 10 10 0.971 10 10 0.912
10 10 0.853 10 10 <0.001 10 10 0.002 10 10 <0.001
10 10 <0.001 10 10 0.529 10 10 0.063 10 9 0.017
10 10 0.003 10 10 0.029 10 10 0.001 10 10 0.529
group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn p nn p nn
p
nn p
10 10 0.971 10 10 0.853 10 10 0.971 10 10 0.796
10 10 0.001 10 10 0.143 10 10 <0.001 10 10 0.190
10 10 0.143 10 10 0.001 9 10 0.133 9 10 0.001
10 10 0.004 10 10 <0.001 10 10 0.123 10 10 0.315
group 2 vs 3 nn p 10 10 0.971 10 10 0.393 10 10 0.023 10 10 0.029 group 3 vs 6 nn p 10 10 0.971 10 10 0.529 10 10 0.971 10 10 0.123 group 6 vs 7 nn p 10 10 0.796 10 10 0.190 10 10 0.001 10 10 0.315
p<0.05
Page A16 of A172
Appendix to TNO-report of 3M-02
Final version
C3. Pooled lipoprotein profile measurements
04-12-2006
Week 0
Cholesterol
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured cholesterol (mM) pooled sample 1
0.008 0.002 0.021 0.043 0.292 0.295 0.166 0.109 0.087 0.109 0.115 0.147 0.222 0.266 0.257 0.216 0.232 0.295 0.307 0.229 0.147 0.093 0.071 0.040
dilution cholesterol (mM)
2
0.016
2
0.004
2
0.042
2
0.086
2
0.584
2
0.590
2
0.332
2
0.218
2
0.174
2
0.218
2
0.230
2
0.294
2
0.444
2
0.532
2
0.514
2
0.432
2
0.464
2
0.590
2
0.614
2
0.458
2
0.294
2
0.186
2
0.142
2
0.080
measured cholesterol (mM) pooled sample 2
0.012 0.008 0.012 0.068 0.248 0.235 0.131 0.093 0.075 0.078 0.100 0.134 0.188 0.222 0.229 0.194 0.210 0.288 0.288 0.203 0.122 0.090 0.049 0.040
dilution cholesterol (mM)
2
0.024
2
0.016
2
0.024
2
0.136
2
0.496
2
0.470
2
0.262
2
0.186
2
0.150
2
0.156
2
0.200
2
0.268
2
0.376
2
0.444
2
0.458
2
0.388
2
0.420
2
0.576
2
0.576
2
0.406
2
0.244
2
0.180
2
0.098
2
0.080
measured cholesterol (mM) pooled sample 3
0.012 0.015 0.005 0.062 0.270 0.285 0.163 0.144 0.103 0.103 0.134 0.181 0.282 0.314 0.279 0.241 0.238 0.339 0.370 0.270 0.166 0.093 0.059 0.040
dilution cholesterol (mM)
2
0.024
2
0.030
2
0.010
2
0.124
2
0.540
2
0.570
2
0.326
2
0.288
2
0.206
2
0.206
2
0.268
2
0.362
2
0.564
2
0.628
2
0.558
2
0.482
2
0.476
2
0.678
2
0.740
2
0.540
2
0.332
2
0.186
2
0.118
2
0.080
Average plasma cholesterol (mmol/l)
0.021 0.017 0.025 0.115 0.540 0.543 0.307 0.231 0.177 0.193 0.233 0.308 0.461 0.535 0.510 0.434 0.453 0.615 0.643 0.468 0.290 0.184 0.119 0.080
sd
0.005 0.013 0.016 0.026 0.044 0.064 0.039 0.052 0.028 0.033 0.034 0.049 0.095 0.092 0.050 0.047 0.029 0.055 0.086 0.068 0.044 0.003 0.022 0.000
PL
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL (mg/dl) pooled sample 1
0.000 0.000 0.000 0.000 3.776 4.429 2.305 1.706 1.597 1.652 1.978 3.503 5.083 8.623 7.752 7.261 9.059 12.762 15.104 12.436 10.094 5.954 3.939 1.760
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
PL (mM)
0.000 0.000 0.000 0.000 0.058 0.068 0.036 0.026 0.025 0.026 0.031 0.054 0.079 0.133 0.120 0.112 0.140 0.197 0.234 0.192 0.156 0.092 0.061 0.027
measured PL (mg/dl) pooled sample 2
0.000 0.000 0.003 0.984 3.513 4.235 2.584 2.016 1.706 1.861 2.016 3.409 5.732 7.022 8.416 7.435 8.622 15.229 15.177 13.061 9.913 7.704 3.822 2.171
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
PL (mM)
0.000 0.000 0.000 0.015 0.054 0.065 0.040 0.031 0.026 0.029 0.031 0.053 0.089 0.109 0.130 0.115 0.133 0.235 0.235 0.202 0.153 0.119 0.059 0.034
measured PL (mg/dl) pooled sample 3
0.000 0.000 0.000 0.000 4.145 4.199 2.670 1.850 1.468 1.796 2.561 3.708 7.313 9.389 10.044 8.460 10.099 17.036 19.167 15.944 10.918 7.969 4.418 1.632
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
PL (mM)
0.000 0.000 0.000 0.000 0.064 0.065 0.041 0.029 0.023 0.028 0.040 0.057 0.113 0.145 0.155 0.131 0.156 0.263 0.296 0.246 0.169 0.123 0.068 0.025
Average plasma PL(mM)
0.000 0.000 0.000 0.005 0.059 0.066 0.039 0.029 0.025 0.027 0.034 0.055 0.093 0.129 0.135 0.119 0.143 0.232 0.255 0.214 0.159 0.111 0.063 0.029
sd
0.000 0.000 0.000 0.009 0.005 0.002 0.003 0.002 0.002 0.002 0.005 0.002 0.018 0.019 0.018 0.010 0.012 0.033 0.036 0.029 0.008 0.017 0.005 0.004
Page A17 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
week 4
Cholesterol
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured cholesterol (mM)
pool group 1
0.006 0.006 0.006 0.028 0.136 0.139 0.096 0.074 0.071 0.083 0.126 0.197 0.272 0.284 0.241 0.204 0.278 0.442 0.429 0.265 0.151 0.099 0.065 0.040
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 1
0.012 0.012 0.012 0.056 0.272 0.278 0.192 0.148 0.142 0.166 0.252 0.394 0.544 0.568 0.482 0.408 0.556 0.884 0.858 0.530 0.302 0.198 0.130 0.080
measured cholesterol (mM)
pool group 2
0.012 0.009 0.009 0.062 0.185 0.157 0.099 0.074 0.071 0.077 0.120 0.173 0.225 0.231 0.219 0.207 0.253 0.358 0.327 0.204 0.123 0.080 0.049 0.034
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 2
0.024 0.018 0.018 0.124 0.370 0.314 0.198 0.148 0.142 0.154 0.240 0.346 0.450 0.462 0.438 0.414 0.506 0.716 0.654 0.408 0.246 0.160 0.098 0.068
measured cholesterol (mM)
pool group 3
0.009 0.012 0.009 0.031 0.108 0.142 0.089 0.077 0.068 0.083 0.108 0.157 0.204 0.207 0.182 0.179 0.222 0.337 0.352 0.238 0.136 0.086 0.049 0.031
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 3
0.018 0.024 0.018 0.062 0.216 0.284 0.178 0.154 0.136 0.166 0.216 0.314 0.408 0.414 0.364 0.358 0.444 0.674 0.704 0.476 0.272 0.172 0.098 0.062
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured cholesterol (mM)
pool group 4
0.020 0.014 0.027 0.030 0.064 0.058 0.048 0.030 0.027 0.030 0.054 0.107 0.215 0.324 0.324 0.271 0.231 0.203 0.160 0.107 0.064 0.045 0.036 0.024
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 4
0.040 0.028 0.054 0.060 0.128 0.116 0.096 0.060 0.054 0.060 0.108 0.214 0.430 0.648 0.648 0.542 0.462 0.406 0.320 0.214 0.128 0.090 0.072 0.048
measured cholesterol (mM)
pool group 5
0.008 0.008 0.005 0.024 0.076 0.079 0.051 0.036 0.033 0.030 0.030 0.061 0.132 0.209 0.259 0.231 0.209 0.209 0.175 0.116 0.073 0.039 0.036 0.017
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 5
0.016 0.016 0.010 0.048 0.152 0.158 0.102 0.072 0.066 0.060 0.060 0.122 0.264 0.418 0.518 0.462 0.418 0.418 0.350 0.232 0.146 0.078 0.072 0.034
measured cholesterol (mM)
pool group 6
0.011 0.008 0.011 0.024 0.054 0.073 0.039 0.036 0.027 0.030 0.036 0.064 0.135 0.215 0.249 0.218 0.197 0.184 0.160 0.110 0.064 0.039 0.027 0.014
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 6
0.022 0.016 0.022 0.048 0.108 0.146 0.078 0.072 0.054 0.060 0.072 0.128 0.270 0.430 0.498 0.436 0.394 0.368 0.320 0.220 0.128 0.078 0.054 0.028
measured cholesterol (mM)
pool group 7
0.016 0.013 0.010 0.025 0.120 0.126 0.065 0.049 0.031 0.034 0.043 0.095 0.246 0.436 0.525 0.458 0.359 0.277 0.203 0.129 0.083 0.049 0.040 0.025
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 7
0.032 0.026 0.020 0.050 0.240 0.252 0.130 0.098 0.062 0.068 0.086 0.190 0.492 0.872 1.050 0.916 0.718 0.554 0.406 0.258 0.166 0.098 0.080 0.050
Page A18 of A172
Appendix to TNO-report of 3M-02
Final version
PL
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL(mg/dl) pool group 1
0.000 0.000 0.000 0.000 0.113 0.247 0.000 0.000 0.000 0.000 0.918 3.201 5.752 7.095 6.289 6.155 12.063 22.804 23.744 14.748 8.303 4.946 2.261 0.247
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 1
0.000 0.000 0.000 0.000 0.002 0.004 0.000 0.000 0.000 0.000 0.014 0.049 0.089 0.110 0.097 0.095 0.186 0.353 0.367 0.228 0.128 0.076 0.035 0.004
measured PL(mg/dl) pool group 2
0.000 0.000 0.000 0.000 1.321 1.187 0.000 0.000 0.000 0.000 0.650 2.664 4.275 5.886 5.618 5.886 10.317 17.702 17.433 10.586 5.752 3.872 1.187 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 2
0.000 0.000 0.000 0.000 0.020 0.018 0.000 0.000 0.000 0.000 0.010 0.041 0.066 0.091 0.087 0.091 0.160 0.274 0.270 0.164 0.089 0.060 0.018 0.000
04-12-2006
measured PL(mg/dl) pool group 3
0.000 0.000 0.000 0.000 0.000 0.516 0.000 0.000 0.000 0.000 0.381 1.992 3.604 4.141 4.409 4.678 8.840 17.031 19.448 12.331 7.363 4.812 1.321 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 3
0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.006 0.031 0.056 0.064 0.068 0.072 0.137 0.263 0.301 0.191 0.114 0.074 0.020 0.000
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL(mg/dl) pool group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.939 4.149 8.162 9.232 8.162 6.557 6.825 5.487 2.946 1.340 0.939 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.064 0.126 0.143 0.126 0.101 0.106 0.085 0.046 0.021 0.015 0.000 0.000
measured PL(mg/dl) pool group 5
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 1.742 4.551 6.825 6.825 6.022 7.226 6.022 3.481 1.875 0.672 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 5
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.027 0.070 0.106 0.106 0.093 0.112 0.093 0.054 0.029 0.010 0.000 0.000
measured PL(mg/dl) pool group 6
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 1.742 4.283 6.022 5.621 5.219 5.487 5.086 2.812 1.207 0.538 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 6
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.027 0.066 0.093 0.087 0.081 0.085 0.079 0.043 0.019 0.008 0.000 0.000
measured PL(mg/dl) pool group 7
0.000 0.000 0.000 0.000 0.000 0.187 0.000 0.000 0.000 0.000 0.000 0.858 5.690 11.998 16.024 15.085 12.400 10.253 7.435 4.348 2.603 1.529 0.321 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 7
0.000 0.000 0.000 0.000 0.000 0.003 0.000 0.000 0.000 0.000 0.000 0.013 0.088 0.185 0.248 0.233 0.192 0.159 0.115 0.067 0.040 0.024 0.005 0.000
Page A19 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Week 6
Cholesterol
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured cholesterol (mM)
pool group 1
0.010 0.001 0.004 0.025 0.135 0.135 0.083 0.065 0.056 0.074 0.129 0.223 0.293 0.293 0.238 0.183 0.241 0.368 0.347 0.220 0.132 0.092 0.062 0.044
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 1
0.020 0.002 0.008 0.050 0.270 0.270 0.166 0.130 0.112 0.148 0.258 0.446 0.586 0.586 0.476 0.366 0.482 0.736 0.694 0.440 0.264 0.184 0.124 0.088
measured cholesterol (mM)
pool group 2
0.007 0.007 0.004 0.050 0.174 0.147 0.083 0.065 0.050 0.068 0.114 0.186 0.226 0.211 0.180 0.162 0.220 0.274 0.241 0.153 0.086 0.059 0.035 0.022
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 2
0.014 0.014 0.008 0.100 0.348 0.294 0.166 0.130 0.100 0.136 0.228 0.372 0.452 0.422 0.360 0.324 0.440 0.548 0.482 0.306 0.172 0.118 0.070 0.044
measured cholesterol (mM)
pool group 3
0.013 0.013 0.010 0.062 0.189 0.168 0.092 0.077 0.062 0.083 0.123 0.180 0.223 0.211 0.162 0.156 0.235 0.329 0.296 0.189 0.114 0.074 0.047 0.032
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 3
0.026 0.026 0.020 0.124 0.378 0.336 0.184 0.154 0.124 0.166 0.246 0.360 0.446 0.422 0.324 0.312 0.470 0.658 0.592 0.378 0.228 0.148 0.094 0.064
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured cholesterol (mM)
pool group 4
0.016 0.004 0.010 0.022 0.068 0.056 0.034 0.031 0.025 0.028 0.059 0.138 0.251 0.315 0.300 0.217 0.168 0.129 0.080 0.056 0.037 0.028 0.025 0.019
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 4
0.032 0.008 0.020 0.044 0.136 0.112 0.068 0.062 0.050 0.056 0.118 0.276 0.502 0.630 0.600 0.434 0.336 0.258 0.160 0.112 0.074 0.056 0.050 0.038
measured cholesterol (mM)
pool group 5
0.010 0.004 0.010 0.077 0.248 0.193 0.117 0.080 0.071 0.077 0.129 0.220 0.281 0.251 0.202 0.156 0.205 0.281 0.254 0.150 0.092 0.056 0.043 0.037
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 5
0.020 0.008 0.020 0.154 0.496 0.386 0.234 0.160 0.142 0.154 0.258 0.440 0.562 0.502 0.404 0.312 0.410 0.562 0.508 0.300 0.184 0.112 0.086 0.074
measured cholesterol (mM)
pool group 6
0.016 0.025 0.016 0.037 0.071 0.071 0.037 0.034 0.031 0.037 0.053 0.120 0.214 0.266 0.266 0.211 0.156 0.126 0.080 0.049 0.037 0.025 0.016 0.013
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 6
0.032 0.050 0.032 0.074 0.142 0.142 0.074 0.068 0.062 0.074 0.106 0.240 0.428 0.532 0.532 0.422 0.312 0.252 0.160 0.098 0.074 0.050 0.032 0.026
measured cholesterol (mM)
pool group 7
0.008 0.011 0.011 0.029 0.093 0.081 0.051 0.045 0.036 0.045 0.075 0.142 0.306 0.425 0.407 0.306 0.206 0.139 0.081 0.054 0.039 0.033 0.026 0.017
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 7
0.016 0.022 0.022 0.058 0.186 0.162 0.102 0.090 0.072 0.090 0.150 0.284 0.612 0.850 0.814 0.612 0.412 0.278 0.162 0.108 0.078 0.066 0.052 0.034
Page A20 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
PL
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL(mg/dl) pool group 1
0.000 0.000 0.000 0.000 1.035 1.166 0.246 0.115 0.115 1.035 2.349 4.977 6.948 7.605 6.685 6.685 11.415 19.168 18.248 11.678 7.342 4.977 2.086 0.772
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 1
0.000 0.000 0.000 0.000 0.016 0.018 0.004 0.002 0.002 0.016 0.036 0.077 0.107 0.118 0.103 0.103 0.176 0.296 0.282 0.181 0.114 0.077 0.032 0.012
measured PL(mg/dl) pool group 2
0.000 0.000 0.000 0.000 1.823 1.297 0.903 0.640 0.246 0.509 1.823 4.057 5.502 6.159 5.897 6.028 9.313 13.649 12.729 8.525 4.714 2.874 1.035 0.115
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 2
0.000 0.000 0.000 0.000 0.028 0.020 0.014 0.010 0.004 0.008 0.028 0.063 0.085 0.095 0.091 0.093 0.144 0.211 0.197 0.132 0.073 0.044 0.016 0.002
measured PL(mg/dl) pool group 3
0.000 0.000 0.000 0.000 1.429 1.429 0.115 0.115 0.000 0.640 2.743 3.400 4.451 4.845 4.188 5.108 9.050 14.963 14.306 9.182 5.239 3.268 1.035 0.246
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 3
0.000 0.000 0.000 0.000 0.022 0.022 0.002 0.002 0.000 0.010 0.042 0.053 0.069 0.075 0.065 0.079 0.140 0.231 0.221 0.142 0.081 0.051 0.016 0.004
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL(mg/dl) pool group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.505 2.599 6.264 9.405 9.798 7.835 5.872 4.301 2.861 1.683 1.291 0.636 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.008 0.040 0.097 0.145 0.151 0.121 0.091 0.066 0.044 0.026 0.020 0.010 0.000 0.000
measured PL(mg/dl) pool group 5
0.000 0.000 0.000 0.113 2.338 1.945 0.898 0.505 0.505 0.898 2.730 5.479 7.442 7.442 6.133 6.002 8.489 12.808 12.547 8.227 5.217 2.992 1.160 0.243
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 5
0.000 0.000 0.000 0.002 0.036 0.030 0.014 0.008 0.008 0.014 0.042 0.085 0.115 0.115 0.095 0.093 0.131 0.198 0.194 0.127 0.081 0.046 0.018 0.004
measured PL(mg/dl) pool group 6
0.000 0.000 0.000 0.000 0.000 0.505 0.000 0.000 0.000 0.000 0.374 2.338 5.348 7.966 8.882 7.049 5.610 4.039 2.599 1.552 0.898 0.505 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 6
0.000 0.000 0.000 0.000 0.000 0.008 0.000 0.000 0.000 0.000 0.006 0.036 0.083 0.123 0.137 0.109 0.087 0.062 0.040 0.024 0.014 0.008 0.000 0.000
measured PL(mg/dl) pool group 7
0.000 0.000 0.000 0.000 0.282 0.414 0.000 0.000 0.000 0.017 0.678 3.583 8.866 13.621 14.413 11.508 7.281 4.640 2.131 1.074 0.678 0.414 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 7
0.000 0.000 0.000 0.000 0.004 0.006 0.000 0.000 0.000 0.000 0.010 0.055 0.137 0.211 0.223 0.178 0.113 0.072 0.033 0.017 0.010 0.006 0.000 0.000
Page A21 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Week 8
Cholesterol
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured cholesterol (mM)
pool group 1
0.000 0.000 0.000 0.036 0.191 0.167 0.103 0.078 0.078 0.100 0.164 0.267 0.328 0.292 0.240 0.191 0.249 0.346 0.346 0.200 0.109 0.066 0.048 0.027
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 1
0.000 0.000 0.000 0.072 0.382 0.334 0.206 0.156 0.156 0.200 0.328 0.534 0.656 0.584 0.480 0.382 0.498 0.692 0.692 0.400 0.218 0.132 0.096 0.054
measured cholesterol (mM)
pool group 2
0.000 0.000 0.002 0.066 0.228 0.182 0.109 0.078 0.069 0.087 0.139 0.200 0.249 0.252 0.237 0.197 0.237 0.276 0.240 0.136 0.078 0.051 0.023 0.020
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 2
0.000 0.000 0.004 0.132 0.456 0.364 0.218 0.156 0.138 0.174 0.278 0.400 0.498 0.504 0.474 0.394 0.474 0.552 0.480 0.272 0.156 0.102 0.046 0.040
measured cholesterol (mM)
pool group 3
0.000 0.011 0.011 0.054 0.206 0.179 0.112 0.091 0.075 0.112 0.164 0.246 0.298 0.273 0.237 0.188 0.231 0.310 0.288 0.170 0.097 0.063 0.030 0.020
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 3
0.000 0.022 0.022 0.108 0.412 0.358 0.224 0.182 0.150 0.224 0.328 0.492 0.596 0.546 0.474 0.376 0.462 0.620 0.576 0.340 0.194 0.126 0.060 0.040
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured cholesterol (mM)
pool group 4
0.015 0.000 0.006 0.021 0.061 0.052 0.023 0.021 0.012 0.035 0.044 0.118 0.271 0.357 0.363 0.280 0.205 0.162 0.107 0.067 0.044 0.023 0.018 0.015
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 4
0.030 0.001 0.012 0.042 0.122 0.104 0.046 0.042 0.024 0.070 0.088 0.236 0.542 0.714 0.726 0.560 0.410 0.324 0.214 0.134 0.088 0.046 0.036 0.030
measured cholesterol (mM)
pool group 5
0.000 0.000 0.006 0.041 0.153 0.130 0.078 0.058 0.049 0.058 0.098 0.159 0.210 0.225 0.208 0.179 0.199 0.257 0.222 0.144 0.075 0.052 0.029 0.029
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 5
0.000 0.000 0.012 0.082 0.306 0.260 0.156 0.116 0.098 0.116 0.196 0.318 0.420 0.450 0.416 0.358 0.398 0.514 0.444 0.288 0.150 0.104 0.058 0.058
measured cholesterol (mM)
pool group 6
0.000 0.006 0.009 0.035 0.107 0.095 0.061 0.044 0.038 0.032 0.067 0.150 0.265 0.372 0.377 0.300 0.239 0.196 0.150 0.092 0.052 0.032 0.021 0.021
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 6
0.001 0.012 0.018 0.070 0.214 0.190 0.122 0.088 0.076 0.064 0.134 0.300 0.530 0.744 0.754 0.600 0.478 0.392 0.300 0.184 0.104 0.064 0.042 0.042
measured cholesterol (mM)
pool group 7
0.000 0.000 0.003 0.041 0.167 0.135 0.073 0.056 0.038 0.050 0.079 0.170 0.302 0.419 0.419 0.328 0.246 0.199 0.138 0.085 0.053 0.035 0.023 0.011
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average cholesterol (mM)
group 7
0.000 0.000 0.006 0.082 0.334 0.270 0.146 0.112 0.076 0.100 0.158 0.340 0.604 0.838 0.838 0.656 0.492 0.398 0.276 0.170 0.106 0.070 0.046 0.022
Page A22 of A172
Appendix to TNO-report of 3M-02
Final version
PL
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL(mg/dl) pool group 1
0.000 0.000 0.000 0.000 2.067 1.800 0.601 0.201 0.334 1.400 3.266 6.599 8.998 8.732 7.132 7.265 11.931 20.595 18.596 11.531 7.265 4.733 2.467 0.867
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 1
0.000 0.000 0.000 0.000 0.032 0.028 0.009 0.003 0.005 0.022 0.050 0.102 0.139 0.135 0.110 0.112 0.184 0.318 0.287 0.178 0.112 0.073 0.038 0.013
measured PL(mg/dl) pool group 2
0.000 0.000 0.000 0.201 3.000 2.067 0.867 0.201 0.334 1.000 2.600 4.999 6.866 7.932 7.665 8.065 10.731 15.130 12.864 8.065 5.266 3.266 1.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 2
0.000 0.000 0.000 0.003 0.046 0.032 0.013 0.003 0.005 0.015 0.040 0.077 0.106 0.123 0.119 0.125 0.166 0.234 0.199 0.125 0.081 0.050 0.015 0.000
04-12-2006
measured PL(mg/dl) pool group 3
0.000 0.000 0.000 0.000 2.200 2.067 0.867 0.334 0.734 1.400 3.533 6.199 8.199 8.732 7.265 7.399 10.864 17.929 15.930 9.798 6.732 4.200 1.534 0.334
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 3
0.000 0.000 0.000 0.000 0.034 0.032 0.013 0.005 0.011 0.022 0.055 0.096 0.127 0.135 0.112 0.114 0.168 0.277 0.246 0.151 0.104 0.065 0.024 0.005
fraction
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
measured PL(mg/dl) pool group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.048 2.583 8.185 12.587 12.987 10.453 8.052 6.184 4.183 2.583 1.916 1.115 0.000 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 4
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.040 0.127 0.195 0.201 0.162 0.124 0.096 0.065 0.040 0.030 0.017 0.000 0.000
measured PL(mg/dl) pool group 5
0.000 0.000 0.000 0.000 1.515 1.249 0.181 0.000 0.000 0.448 1.515 3.916 5.517 6.851 6.851 7.118 9.786 14.454 13.521 8.318 4.450 3.116 0.848 0.315
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 5
0.000 0.000 0.000 0.000 0.023 0.019 0.003 0.000 0.000 0.007 0.023 0.061 0.085 0.106 0.106 0.110 0.151 0.223 0.209 0.129 0.069 0.048 0.013 0.005
measured PL(mg/dl) pool group 6
0.000 0.000 0.000 0.000 0.715 0.582 0.000 0.000 0.000 0.000 0.715 3.783 8.452 12.720 13.120 11.386 9.519 8.318 5.917 3.650 2.983 2.182 0.315 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 6
0.000 0.000 0.000 0.000 0.011 0.009 0.000 0.000 0.000 0.000 0.011 0.058 0.131 0.197 0.203 0.176 0.147 0.129 0.091 0.056 0.046 0.034 0.005 0.000
measured PL(mg/dl) pool group 7
0.000 0.000 0.000 0.000 1.619 0.951 0.150 0.000 0.000 0.000 0.951 4.290 10.033 13.639 14.707 12.570 10.166 8.163 5.626 3.622 2.687 1.619 0.150 0.000
dilution
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
Average PL (mM) group 7
0.000 0.000 0.000 0.000 0.025 0.015 0.002 0.000 0.000 0.000 0.015 0.066 0.155 0.211 0.227 0.194 0.157 0.126 0.087 0.056 0.042 0.025 0.002 0.000
Page A23 of A172
Appendix to TNO-report of 3M-02
Final version
C4. Pooled plasma ALAT measurements
04-12-2006
group
Group 1: HF control Group 2: HF + 0.01 % PFBS Group 3: HF + 0.03 % PFBS Group 4: HF + 0.006 % PFHS Group 5: HF + 0.02 % PFHS Group 6: HF + 0.003 % PFOS Group 7: HF + 0.01 % PFOS
week 0 97.1
measured week 4
27.1 30.6 23.5 88.7 46 109 119
Pooled plasma ALAT (U/l) dilution week 4 week 6 (o/n fast)
3
81.3
84
3
91.8
113
3
70.5
108
3
266.1
213
3
138
130
3
327
246
3
357
307
week 8
57 134 87.2 370 180 394 450
Page A24 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
C5. Plasma ketone bodies measurements
Week -1
concentration
ketonbodies (mmol/l)
0.000 0.000 0.033 0.033 0.067 0.067 0.134 0.134 0.268 0.268 0.535 0.535 1.070 1.070 2.140 2.140
A340 pre
0.214 0.214 0.218 0.215 0.218 0.222 0.216 0.22 0.218 0.215 0.218 0.215 0.217 0.218 0.217 0.22
A340 post
0.211 0.214 0.222 0.22 0.223 0.239 0.249 0.257 0.282 0.284 0.348 0.358 0.462 0.484 0.689 0.716
A340 netto
-0.003 0
0.004 0.005 0.005 0.017 0.033 0.037 0.064 0.069 0.13 0.143 0.245 0.266 0.472 0.496
A340netto
0.6 y = 0.2283x + 0.0028
0.5
0.4
0.3
0.2
0.1
0
0.0
0.5
1.0
1.5
2.0
2.5
Keton bodies (mmol/l)
y=ax + b
mouse#
641 645 646 650 652 655 656 657 658 663 665 666 667 670 671 674 678 679 680 681 686 687 690 696 699 700 704 705 706 710 712 714 716 720 721 724 727 729 730 731 737 738 740 741 742 744 748 752 754 756 758 759 760 761 762 763 765 766 768 769 771 774 776 778 782 783 784 785 790 791 792 793 795 797 799
a:
A340 pre
0.307 0.321
0.3 0.269 0.293 0.329 0.307 0.381 0.293 0.35 0.245 0.276 0.273 0.351 0.324 0.464 0.291 0.447 0.334 0.468 0.352 0.467 0.356 0.274
0.3 0.305 0.311 0.294 0.292 0.419 0.388 0.289 0.333 0.346 0.302 0.347 0.29 0.244 0.317 0.27 0.432 0.387 0.351 0.249 0.239 0.242 0.26 0.284 0.366 0.282 0.333 0.317 0.303 0.293 0.277 0.297 0.287 0.309 0.383 0.382 0.318 0.357 0.31 0.241 0.298 0.282 0.284 0.272 0.253 0.326 0.292 0.261 0.371 0.339 0.301
0.2283
A340 post
0.766 0.546 0.623 0.644 0.546 0.53 0.505 0.67 0.658 0.58 0.489 0.65 0.668 0.621 0.613 0.628 0.534 0.649 0.579 0.736 0.608 0.638 0.644 0.546 0.563 0.583 0.666 0.609 0.621 0.664 0.624 0.573 0.465 0.692 0.705 0.754 0.44 0.37 0.423 0.405 0.712 0.581 0.578 0.485 0.418 0.516 0.429 0.445 0.631 0.558 0.664 0.613 0.605 0.639 0.623 0.69 0.62 0.627 0.632 0.693 0.533 0.554 0.521 0.466 0.596 0.556 0.653 0.645 0.452 0.589 0.58 0.403 0.561 0.712 0.572
b:
0.0028
A340 netto
0.459 0.225 0.323 0.375 0.253 0.201 0.198 0.289 0.365 0.23 0.244 0.374 0.395 0.27 0.289 0.164 0.243 0.202 0.245 0.268 0.256 0.171 0.288 0.272 0.263 0.278 0.355 0.315 0.329 0.245 0.236 0.284 0.132 0.346 0.403 0.407 0.15 0.126 0.106 0.135 0.28 0.194 0.227 0.236 0.179 0.274 0.169 0.161 0.265 0.276 0.331 0.296 0.302 0.346 0.346 0.393 0.333 0.318 0.249 0.311 0.215 0.197 0.211 0.225 0.298 0.274 0.369 0.373 0.199 0.263 0.288 0.142 0.19 0.373 0.271
meas. concentration
ketonbodies (mmol/l)
2.00 0.97 1.40 1.63 1.10 0.87 0.86 1.25 1.59 1.00 1.06 1.63 1.72 1.17 1.25 0.71 1.05 0.87 1.06 1.16 1.11 0.74 1.25 1.18 1.14 1.21 1.54 1.37 1.43 1.06 1.02 1.23 0.57 1.50 1.75 1.77 0.64 0.54 0.45 0.58 1.21 0.84 0.98 1.02 0.77 1.19 0.73 0.69 1.15 1.20 1.44 1.28 1.31 1.50 1.50 1.71 1.45 1.38 1.08 1.35 0.93 0.85 0.91 0.97 1.29 1.19 1.60 1.62 0.86 1.14 1.25 0.61 0.82 1.62 1.17
dilution
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1
Plasma keton-
bodies (mmol/l)
2.00 0.97 1.40 1.63 1.10 0.87 0.86 1.25 1.59 1.00 1.06 1.63 1.72 1.17 1.25 0.71 1.05 0.87 1.06 1.16 1.11 0.74 1.25 1.18 1.14 1.21 1.54 1.37 1.43 1.06 1.02 1.23 0.57 1.50 1.75 1.77 1.29 1.08 0.90 1.16 1.21 0.84 0.98 1.02 0.77 1.19 0.73 1.39 1.15 1.20 1.44 1.28 1.31 1.50 1.50 1.71 1.45 1.38 1.08 1.35 0.93 0.85 0.91 0.97 1.29 1.19 1.60 1.62 1.72 1.14 1.25 0.61 0.82 1.62 1.17
mouse#
after matching
11 12 13 21 61 excluded excluded 62 1 2 41 42 43 63 44 14 22 45 64 31 32 65 23 66 24 46 33 34 35 36 3 51 4 67 52 68 53 25 47 48 15 16 17 excluded excluded 49 excluded 5 26 27 6 28 18 69 70 7 19 29 37 54 38 55 56 57 58 59 8 20 9 10 39 30 40 60 50
Page A25 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Week 6
concentration
ketonbodies (mmol/l)
0.000 0.000 0.033 0.033 0.067 0.067 0.134 0.134 0.268 0.268 0.535 0.535 1.070 1.070 2.140 2.140
A340 pre
0.255 0.254 0.255 0.258 0.256 0.262 0.257 0.262 0.257 0.256 0.258 0.257 0.256 0.261 0.259 0.266
A340 post
0.242 0.246 0.251 0.254 0.261 0.269 0.279 0.287 0.318 0.319 0.388 0.392
0.5 0.521 0.729 0.768
A340 netto
-0.013 -0.008 -0.004 -0.004 0.005 0.007 0.022 0.025 0.061 0.063 0.13 0.135 0.244 0.26 0.47 0.502
A340netto
0.6 y = 0.233x - 0.0053
0.5
0.4
0.3
0.2
0.1
0
0.0
0.5
1.0
1.5
2.0
2.5
Keton bodies (mmol/l)
y=ax + b
Group
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
a:
0.233
Mouse# cage#
1
1
2
1
3
2
4
2
5
3
6
3
7
4
8
5
9
5
10
5
11
6
12
6
13
6
14
7
15
8
16
8
17
8
18
9
19
9
20
10
21
11
22
12
23
13
24
13
25
14
26
15
27
15
28
15
29
16
30
17
31
18
32
18
33
19
34
19
35
19
36
19
37
20
38
20
39
21
40
21
41
22
42
22
43
22
44
22
45
23
46
24
47
25
48
25
49
26
50
27
51
28
52
29
53
29
54
30
55
30
56
31
57
31
58
31
59
31
60
32
61
33
62
34
63
35
64
36
65
36
66
37
67
38
68
38
69
39
70
39
b:
-0.0053
eartag
L
R
0
1
1
2
0
0
2
0
0
0
0
2
1
1
0
0
0
2
1
2
A340 pre
0.303 0.284 0.273 0.271 0.267 0.27 0.283 0.27 0.269 0.28
1
0
0.289
2
1
0.288
0
2
0.273
0
1
0.288
1
0
0.282
0
1
0.299
2
0
0.308
0
0
0.271
2
1
0.267
1
0
0.27
0
1
0.279
0
2
0.277
0
1
0.318
1
1
0.291
1
0
0.262
0
1
0.272
2
0
0.261
1
2
0.269
0
2
0.28
1
0
0.269
1
0
0.262
0
2
0.266
0
0
0.275
1
0
0.275
0
1
0.285
0
2
0.284
0
0
0.263
1
1
0.263
0
0
0.272
1
1
0.273
1
0
0.259
0
1
0.29
1
1
0.299
1
2
0.284
1
2
0.315
2
0
0.305
0
1
0.309
1
1
0.295
0
0
0.253
1
2
0.296
0
1
0.263
1
0
0.261
1
2
0.276
1
0
0.262
0
2
0.262
0
0
0.278
0
1
0.264
0
2
0.274
2
2
0.283
2
1
0.284
2
0
0.266
1
0
0.269
0
2
0.268
0
0
0.268
1
2
0.268
0
0
0.27
0
0
0.268
2
0
0.269
1
0
0.277
0
1
0.274
A340 post 0.443 0.402 0.383 0.359 0.382 0.363 0.424 0.38 0.367 0.379
0.413 0.343 0.382 0.468 0.395 0.347 0.364 0.413 0.366 0.383
0.443 0.473 0.409 0.38 0.421 0.418 0.419 0.374 0.445 0.372
0.409 0.381 0.424 0.439 0.452 0.446 0.417 0.365 0.49 0.427
0.435 0.437 0.541 0.408 0.558 0.529 0.399 0.439 0.44 0.466
0.581 0.556 0.541 0.54 0.551 0.605 0.408 0.605 0.564 0.659
0.551 0.568 0.566 0.522 0.541 0.579 0.615 0.568 0.618 0.647
A340 netto 0.14 0.118 0.11 0.088 0.115 0.093 0.141 0.11 0.098 0.099
0.124 0.055 0.109 0.18 0.113 0.048 0.056 0.142 0.099 0.113
0.164 0.196 0.091 0.089 0.159 0.146 0.158 0.105 0.165 0.103
0.147 0.115 0.149 0.164 0.167 0.162 0.154 0.102 0.218 0.154
0.176 0.147 0.242 0.124 0.243 0.224 0.09 0.144 0.187 0.17
0.318 0.295 0.265 0.278 0.289 0.327 0.144 0.331 0.281 0.375
0.285 0.299 0.298 0.254 0.273 0.309 0.347 0.299 0.341 0.373
meas. concentration ketonbodies (mmol/l)
0.62 0.53 0.49 0.40 0.52 0.42 0.63 0.49 0.44 0.45
0.55 0.26 0.49 0.80 0.51 0.23 0.26 0.63 0.45 0.51
0.73 0.86 0.41 0.40 0.71 0.65 0.70 0.47 0.73 0.46
0.65 0.52 0.66 0.73 0.74 0.72 0.68 0.46 0.96 0.68
0.78 0.65 1.06 0.55 1.07 0.98 0.41 0.64 0.83 0.75
1.39 1.29 1.16 1.22 1.26 1.43 0.64 1.44 1.23 1.63
1.25 1.31 1.30 1.11 1.19 1.35 1.51 1.31 1.49 1.62
dilution
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
3 3 3 3 3 3 3 3 3 3
Plasma keton-
bodies (mmol/l)
1.87 1.59 1.48 1.20 1.55 1.27 1.88 1.48 1.33 1.34 1.50 0.23 1.66 0.78 1.47 2.39 1.52 0.69 0.79 1.90 1.34 1.52 1.41 0.54 2.18 2.59 1.24 1.21 2.12 1.95 2.10 1.42 2.19 1.39 1.84 0.48 1.96 1.55 1.99 2.18 2.22 2.15 2.05 1.38 2.88 2.05
2.04 0.40
2.33 1.96 3.18 1.66 3.20 2.95 1.23 1.92 2.48 2.26
2.32 0.65
4.16 3.87 3.48 3.65 3.79 4.28 1.92 4.33 3.69 4.90
3.81 0.78 3.74 3.92 3.91 3.34 3.58 4.05 4.54 3.92 4.46 4.87 4.03 0.47
Page A26 of A172
Appendix to TNO-report of 3M-02
Final version
Group
Mouse# cage#
1
1
1
1
2
1
1
3
2
1
4
2
1
5
3
1
6
3
1
7
4
1
8
5
1
9
5
1
10
5
AVERAGE
SD
2
11
6
2
12
6
2
13
6
2
14
7
2
15
8
2
16
8
2
17
8
2
18
9
2
19
9
2
20
10
AVERAGE
SD
3
21
11
3
22
12
3
23
13
3
24
13
3
25
14
3
26
15
3
27
15
3
28
15
3
29
16
3
30
17
AVERAGE
SD
4
31
18
4
32
18
4
33
19
4
34
19
4
35
19
4
36
19
4
37
20
4
38
20
4
39
21
4
40
21
AVERAGE
SD
5
41
22
5
42
22
5
43
22
5
44
22
5
45
23
5
46
24
5
47
25
5
48
25
5
49
26
5
50
27
AVERAGE
SD
6
51
28
6
52
29
6
53
29
6
54
30
6
55
30
6
56
31
6
57
31
6
58
31
6
59
31
6
60
32
AVERAGE
SD
7
61
33
7
62
34
7
63
35
7
64
36
7
65
36
7
66
37
7
67
38
7
68
38
7
69
39
7
70
39
AVERAGE
SD
excluded (655)
excluded (656)
excluded (741)
excluded (742)
excluded (748)
eartag LR 01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
Plasma ketonbodies (mmol/l)
week -1
week 6
1.59
1.87
1.00
1.59
1.02
1.48
0.57
1.20
1.39
1.55
1.44
1.27
1.71
1.88
1.60
1.48
1.72
1.33
1.14
1.34
1.32
1.50
0.38
0.23
2.00
1.66
0.97
0.78
1.40
1.47
0.71
2.39
1.21
1.52
0.84
0.69
0.98
0.79
1.31
1.90
1.45
1.34
1.62
1.52
1.25
1.41
0.39
0.54
1.63
2.18
1.05
2.59
1.25
1.24
1.14
1.21
1.08
2.12
1.15
1.95
1.20
2.10
1.28
1.42
1.38
2.19
0.61
1.39
1.18
1.84
0.26
0.48
1.16
1.96
1.11
1.55
1.54
1.99
1.37
2.18
1.43
2.22
1.06
2.15
1.08
2.05
0.93
1.38
1.25
2.88
0.82
2.05
1.17
2.04
0.22
0.40
1.06
2.33
1.63
1.96
1.72
3.18
1.25
1.66
0.87
3.20
1.21
2.95
0.90
1.23
1.16
1.92
1.19
2.48
1.17
2.26
1.22
2.32
0.27
0.65
1.23
4.16
1.75
3.87
1.29
3.48
1.35
3.65
0.85
3.79
0.91
4.28
0.97
1.92
1.29
4.33
1.19
3.69
1.62
4.90
1.25
3.81
0.29
0.78
1.10
3.74
1.25
3.92
1.17
3.91
1.06
3.34
0.74
3.58
1.18
4.05
1.50
4.54
1.77
3.92
1.50
4.46
1.50
4.87
1.28
4.03
0.30
0.47
0.87
0.86
1.02
0.77
0.73
04-12-2006
Page A27 of A172
Appendix to TNO-report of 3M-02
Final version
Appendix D. Measurements in metabolic cages D1. Code mice and metabolic cages
group# mouse# metabolic cage#
1
2
101
1
3
105
1
4
201
1
6
205
3
21
102
3
22
106
3
24
202
3
27
206
4
31
103
4
32
107
4
37
203
4
38
207
6
51
104
6
53
108
6
54
204
6
55
208
04-12-2006
D2. Average bodyweight mice in metabolic cages
group# mouse#
1
2
1
3
1
4
1
6
3
21
3
22
3
24
3
27
4
31
4
32
4
37
4
38
6
51
6
53
6
54
6
55
metabolic cage#
101 105 201 205 102 106 202 206 103 107 203 207 104 108 204 208
Bodyweight (g)
begin metabolic cage end metabolic cage
29.9
30.7
30.2
30
28
29.1
29.3
29.6
29.5
30.2
29.8
30.1
28.8
29.7
29.5
30.2
28.9
29.8
28.9
29
26.9
28.9
30.4
31.3
28.2
29.1
27.3
26.5
29.4
30.7
27.8
28.8
Average bodyweight (g) in metabolic cage 30.30 30.10 28.55 29.45
29.85 29.95 29.25 29.85
29.35 28.95 27.90 30.85
28.65 26.90 30.05 28.30
Page A28 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
D3. Food and drink intake measurements
Food intake measurements
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1: HF control
Accumulated food intake (g)
Average acc. SD
cage 0101 cage 0105 cage 0201 cage 0205 food intake (g) (g)
0.258
0.182
0.114
0.206
0.19
0.06
0.735
0.468
0.273
0.555
0.51
0.19
0.888
0.696
0.733
0.777
0.77
0.08
1.017
0.851
0.926
0.997
0.95
0.08
1.205
0.946
1.021
1.184
1.09
0.13
1.380
1.248
1.356
1.345
1.33
0.06
1.571
1.388
1.770
1.525
1.56
0.16
1.810
1.510
2.016
1.681
1.75
0.21
2.015
1.682
2.182
1.792
1.92
0.22
2.184
1.747
2.294
1.898
2.03
0.25
2.402
1.837
2.324
1.992
2.14
0.27
2.458
1.940
2.623
2.060
2.27
0.32
2.616
2.045
3.224
2.152
2.51
0.54
2.724
2.188
3.390
2.308
2.65
0.54
2.824
2.437
3.480
2.473
2.80
0.48
3.064
2.599
3.486
2.689
2.96
0.40
3.300
2.792
3.510
2.748
3.09
0.38
3.564
3.013
3.525
2.995
3.27
0.31
3.879
3.109
3.676
3.304
3.49
0.35
4.146
3.214
3.802
3.448
3.65
0.41
4.399
3.284
3.980
3.750
3.85
0.46
4.531
3.372
4.110
3.918
3.98
0.48
4.743
3.412
4.174
3.977
4.08
0.55
4.856
3.514
4.280
4.085
4.18
0.55
4.935
3.602
4.321
4.343
4.30
0.55
5.129
3.739
4.441
4.488
4.45
0.57
5.509
4.064
4.469
4.635
4.67
0.61
5.770
4.341
4.705
4.815
4.91
0.61
5.968
4.531
4.897
4.947
5.09
0.62
6.096
4.684
5.032
5.059
5.22
0.61
6.260
4.869
5.144
5.138
5.35
0.62
6.378
5.016
5.351
5.318
5.52
0.59
6.529
5.181
5.535
5.470
5.68
0.59
6.741
5.397
5.692
5.729
5.89
0.59
6.916
5.608
5.944
5.965
6.11
0.56
7.046
5.794
6.060
6.139
6.26
0.54
7.233
5.993
6.328
6.449
6.50
0.52
7.443
6.101
6.615
6.749
6.73
0.55
7.561
6.231
6.776
6.879
6.86
0.55
7.629
6.317
7.002
7.007
6.99
0.54
7.763
6.406
7.175
7.153
7.12
0.56
8.003
6.555
7.392
7.280
7.31
0.59
8.140
6.690
7.504
7.345
7.42
0.60
8.297
6.796
7.656
7.432
7.55
0.62
8.485
6.840
7.831
7.553
7.68
0.68
8.671
7.034
7.922
7.620
7.81
0.68
8.766
7.102
7.973
7.671
7.88
0.69
8.926
7.156
8.014
7.735
7.96
0.74
9.030
7.190
8.075
7.826
8.03
0.76
9.096
7.277
8.149
7.856
8.09
0.76
9.128
7.336
8.299
7.904
8.17
0.75
9.139
7.553
8.481
8.007
8.30
0.68
9.279
7.633
8.613
8.157
8.42
0.70
9.441
7.933
8.781
8.277
8.61
0.66
9.564
8.168
8.938
8.488
8.79
0.61
9.710
8.462
9.207
8.706
9.02
0.55
9.835
8.621
9.459
8.970
9.22
0.53
10.044 8.689
9.575
9.184
9.37
0.58
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
Accumulated food intake (g)
Average acc. SD
cage 0102 cage 0106 cage 0202 cage 0206 food intake (g) (g)
0.215
0.190
0.188
0.203
0.20
0.01
0.548
0.528
0.643
0.494
0.55
0.06
0.834
0.665
0.961
0.615
0.77
0.16
0.979
0.822
1.200
0.745
0.94
0.20
1.156
1.081
1.312
0.890
1.11
0.18
1.416
1.340
1.505
1.058
1.33
0.19
1.624
1.437
1.738
1.282
1.52
0.20
1.828
1.652
1.911
1.374
1.69
0.24
2.005
1.696
2.105
1.503
1.83
0.28
2.164
1.772
2.282
1.601
1.95
0.32
2.200
1.844
2.450
1.658
2.04
0.35
2.290
1.877
2.731
1.761
2.16
0.44
2.416
2.008
2.955
1.807
2.30
0.51
2.506
2.171
3.097
1.885
2.41
0.52
2.638
2.304
3.284
2.120
2.59
0.51
2.790
2.564
3.633
2.294
2.82
0.58
3.033
2.637
3.819
2.454
2.99
0.61
3.288
2.836
4.141
2.726
3.25
0.64
3.478
3.028
4.557
2.931
3.50
0.74
3.658
3.030
4.845
3.087
3.66
0.84
3.815
3.033
4.958
3.251
3.76
0.86
3.989
3.083
5.184
3.466
3.93
0.91
4.160
3.140
5.331
3.592
4.06
0.95
4.264
3.200
5.478
3.741
4.17
0.97
4.385
3.322
5.596
3.824
4.28
0.98
4.660
3.464
5.823
4.060
4.50
1.01
5.208
3.675
6.138
4.316
4.83
1.07
5.472
3.794
6.381
4.484
5.03
1.13
5.665
4.016
6.645
4.648
5.24
1.16
5.794
4.175
6.781
4.691
5.36
1.16
5.890
4.375
6.887
4.736
5.47
1.14
6.059
4.476
7.033
4.870
5.61
1.16
6.241
4.642
7.204
4.965
5.76
1.18
6.399
4.839
7.392
5.180
5.95
1.17
6.520
5.095
7.600
5.323
6.13
1.16
6.764
5.403
7.862
5.512
6.39
1.16
7.101
5.597
8.127
5.755
6.65
1.20
7.424
5.802
8.354
6.043
6.91
1.20
7.608
5.958
8.528
6.120
7.05
1.23
7.838
6.095
8.658
6.304
7.22
1.23
8.024
6.240
8.807
6.420
7.37
1.25
8.114
6.300
8.955
6.534
7.48
1.27
8.228
6.388
9.050
6.656
7.58
1.27
8.312
6.460
9.159
6.748
7.67
1.28
8.479
6.546
9.299
6.817
7.79
1.32
8.672
6.724
9.494
6.931
7.96
1.35
8.711
6.908
9.698
6.987
8.08
1.36
8.785
7.060
9.857
7.086
8.20
1.37
8.830
7.178
9.942
7.105
8.26
1.37
8.844
7.263 10.006 7.200
8.33
1.35
8.903
7.332 10.105 7.265
8.40
1.36
8.920
7.393 10.230 7.319
8.47
1.39
8.996
7.574 10.390 7.448
8.60
1.38
9.098
7.806 10.490 7.629
8.76
1.33
9.384
7.976 10.763 7.816
8.98
1.38
9.629
8.172 11.070 8.089
9.24
1.41
9.819
8.314 11.308 8.285
9.43
1.44
9.936
8.454 11.426 8.447
9.57
1.42
Page A29 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Metabolic cages group 4: HF + 0.006 % PFHS
Time
Accumulated food intake (g)
Average acc.
SD
(hr) cage 0103 cage 0107 cage 0203 cage 0207 food intake (g)
(g)
6.1
0.211
0.360
0.336
0.424
0.33
0.09
7.4
0.535
0.688
0.784
0.907
0.73
0.16
8.6
0.656
0.881
1.069
1.157
0.94
0.22
9.9
0.844
1.075
1.187
1.292
1.10
0.19
11.1
0.952
1.146
1.318
1.510
1.23
0.24
12.4
1.045
1.282
1.490
1.612
1.36
0.25
13.6
1.181
1.410
1.611
1.682
1.47
0.23
14.9
1.355
1.470
1.719
1.825
1.59
0.22
16.1
1.475
1.518
1.796
1.867
1.66
0.20
17.4
1.612
1.603
1.896
2.072
1.80
0.23
18.6
1.724
1.856
2.060
2.173
1.95
0.20
19.9
1.840
2.142
2.138
2.328
2.11
0.20
21.1
2.066
2.412
2.257
2.463
2.30
0.18
22.4
2.272
2.630
2.440
2.629
2.49
0.17
23.6
2.408
2.775
2.575
2.771
2.63
0.18
24.9
2.588
2.822
2.849
2.957
2.80
0.16
26.1
2.702
2.952
3.090
3.204
2.99
0.22
27.4
2.899
3.350
3.486
3.580
3.33
0.30
28.6
3.247
3.880
3.988
4.074
3.80
0.38
29.9
3.592
4.090
4.394
4.524
4.15
0.41
31.1
3.788
4.209
4.849
4.851
4.42
0.52
32.4
3.849
4.315
5.167
5.095
4.61
0.64
33.6
3.935
4.435
5.255
5.180
4.70
0.63
34.9
4.033
4.530
5.345
5.222
4.78
0.62
36.1
4.112
4.596
5.513
5.308
4.88
0.65
37.4
4.297
4.917
5.702
5.423
5.08
0.62
38.6
4.563
5.184
5.847
5.572
5.29
0.56
39.9
4.733
5.370
5.977
5.836
5.48
0.56
41.1
4.925
5.644
6.195
5.974
5.68
0.55
42.4
5.058
5.862
6.305
6.104
5.83
0.55
43.6
5.163
6.025
6.418
6.208
5.95
0.55
44.9
5.285
6.249
6.561
6.264
6.09
0.56
46.1
5.412
6.429
6.782
6.450
6.27
0.59
47.4
5.592
6.682
7.023
6.601
6.47
0.62
48.6
5.945
6.866
7.303
6.770
6.72
0.57
49.9
6.233
7.064
7.717
6.965
6.99
0.61
51.1
6.686
7.269
8.032
7.365
7.34
0.55
52.4
7.036
7.546
8.318
7.866
7.69
0.54
53.6
7.312
7.920
8.558
8.118
7.98
0.52
54.9
7.437
8.042
8.678
8.400
8.14
0.54
56.1
7.544
8.134
8.803
8.589
8.27
0.56
57.4
7.578
8.166
8.931
8.735
8.35
0.61
58.6
7.667
8.205
9.030
8.832
8.43
0.62
59.9
7.792
8.260
9.197
8.900
8.54
0.63
61.1
7.871
8.338
9.339
9.022
8.64
0.66
62.4
7.998
8.416
9.506
9.156
8.77
0.69
63.6
8.093
8.503
9.620
9.257
8.87
0.70
64.9
8.272
8.585
9.680
9.388
8.98
0.66
66.1
8.345
8.669
9.753
9.484
9.06
0.66
67.4
8.426
8.750
9.904
9.569
9.16
0.69
68.6
8.474
8.848 10.001 9.658
9.25
0.71
69.9
8.596
8.900 10.123 9.772
9.35
0.72
71.1
8.664
9.017 10.340 9.883
9.48
0.77
72.4
8.858
9.148 10.445 10.118
9.64
0.76
73.6
9.226
9.256 10.689 10.283
9.86
0.74
74.9
9.590
9.385 10.994 10.529
10.12
0.76
76.1
9.961
9.515 11.208 10.865
10.39
0.78
77.4
10.204 9.712 11.433 11.104
10.61
0.79
Metabolic cages group 6: HF + 0.003 % PFOS
Time
Accumulated food intake (g)
Average acc.
SD
(hr) cage 0104 cage 0108 cage 0204 cage 0208 food intake (g)
(g)
6.1
0.204
0.119
0.211
0.256
0.20
0.06
7.4
0.535
0.170
0.629
0.547
0.47
0.20
8.6
0.808
0.260
0.916
0.709
0.67
0.29
9.9
0.960
0.518
1.134
0.865
0.87
0.26
11.1
1.185
0.732
1.406
1.047
1.09
0.28
12.4
1.512
0.847
1.618
1.189
1.29
0.35
13.6
1.749
0.889
1.848
1.340
1.46
0.44
14.9
1.940
0.998
2.001
1.406
1.59
0.47
16.1
2.020
1.095
2.100
1.591
1.70
0.46
17.4
2.174
1.216
2.185
1.642
1.80
0.47
18.6
2.406
1.304
2.252
1.767
1.93
0.50
19.9
2.543
1.370
2.270
1.851
2.01
0.51
21.1
2.648
1.558
2.390
1.938
2.13
0.48
22.4
2.810
1.650
2.480
2.082
2.26
0.50
23.6
3.050
1.750
2.546
2.234
2.40
0.55
24.9
3.301
1.898
2.689
2.577
2.62
0.57
26.1
3.423
2.071
2.851
2.815
2.79
0.55
27.4
3.778
2.310
3.211
3.149
3.11
0.60
28.6
4.101
2.641
3.553
3.436
3.43
0.60
29.9
4.314
2.965
3.990
3.637
3.73
0.58
31.1
4.529
3.048
4.316
3.864
3.94
0.66
32.4
4.663
3.078
4.572
3.978
4.07
0.73
33.6
4.720
3.203
4.695
4.070
4.17
0.71
34.9
4.865
3.307
5.002
4.232
4.35
0.77
36.1
4.989
3.385
5.161
4.322
4.46
0.81
37.4
5.264
3.615
5.358
4.501
4.68
0.81
38.6
5.554
3.670
5.466
4.702
4.85
0.87
39.9
5.835
3.735
5.640
4.919
5.03
0.95
41.1
6.067
3.862
5.747
5.126
5.20
0.97
42.4
6.303
3.946
5.878
5.205
5.33
1.03
43.6
6.441
4.055
6.075
5.268
5.46
1.06
44.9
6.584
4.147
6.188
5.421
5.59
1.07
46.1
6.733
4.293
6.320
5.543
5.72
1.07
47.4
7.107
4.400
6.508
5.644
5.91
1.17
48.6
7.672
4.522
6.617
5.846
6.16
1.33
49.9
7.929
4.758
6.854
6.035
6.39
1.34
51.1
8.198
5.100
7.194
6.268
6.69
1.32
52.4
8.427
5.352
7.512
6.465
6.94
1.33
53.6
8.566
5.511
7.721
6.688
7.12
1.32
54.9
8.762
5.603
7.901
6.921
7.30
1.36
56.1
8.892
5.689
8.062
7.030
7.42
1.38
57.4
9.088
5.749
8.139
7.093
7.52
1.43
58.6
9.207
5.852
8.244
7.166
7.62
1.44
59.9
9.378
6.024
8.302
7.200
7.73
1.44
61.1
9.554
6.105
8.386
7.307
7.84
1.48
62.4
9.689
6.228
8.526
7.433
7.97
1.48
63.6
9.839
6.294
8.674
7.524
8.08
1.52
64.9
10.003 6.361
8.830
7.611
8.20
1.57
66.1
10.139 6.478
8.880
7.664
8.29
1.58
67.4
10.256 6.542
8.933
7.717
8.36
1.60
68.6
10.347 6.591
9.006
7.720
8.42
1.62
69.9
10.464 6.718
9.083
7.740
8.50
1.63
71.1
10.593 6.810
9.309
7.807
8.63
1.66
72.4
10.793 6.915
9.451
7.914
8.77
1.71
73.6
11.011 7.103
9.579
8.188
8.97
1.70
74.9
11.264 7.345
9.788
8.977
9.34
1.63
76.1
11.518 7.679 10.107 9.184
9.62
1.61
77.4
11.641 7.878 10.312 9.426
9.81
1.58
Page A30 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000 4 4 1.000 4 4 1.000 4 4 1.000 4 4 1.000 4 4 0.860 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.886 4 4 1.000 4 4 1.000 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886
Accumulated Food intake; p-values Mann Whitney U-test group 1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6 nn p nn p nn p nn p nn p 4 4 0.057 4 4 0.860 4 4 0.057 4 4 0.686 4 4 0.057 4 4 0.200 4 4 0.886 4 4 0.114 4 4 0.886 4 4 0.114 4 4 0.486 4 4 1.000 4 4 0.343 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.200 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.343 4 4 0.200 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.343 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.486 4 4 0.486 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.114 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.057 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.114 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.114 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.057 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.057 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.686
p<0.05
Page A31 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Feeding bout size measurements
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
cage 0101 0.045 0.043 0.002 0.018 0.017 0.022 0.024 0.023 0.013 0.014 0.023 0.006 0.019 0.008 0.017 0.016 0.038 0.012 0.040 0.029 0.015 0.025 0.011 0.007 0.016 0.031 0.026 0.026 0.018 0.012 0.021 0.008 0.018 0.023 0.009 0.021 0.025 0.010 0.008 0.011 0.013 0.027 0.019 0.007 0.029 0.011 0.002 0.024 0.002 0.008 0.001 0.002 0.017 0.018 0.009 0.019 0.021 0.010
Metabolic cages group 1: HF control
Feeding bout size (g)
Average feeding
cage 0105 cage 0201 cage 0205 bout size (g)
0.036
0.018
0.044
0.036
0.019
0.032
0.025
0.030
0.029
0.034
0.018
0.021
0.002
0.012
0.025
0.014
0.014
0.010
0.008
0.012
0.032
0.053
0.022
0.032
0.013
0.038
0.017
0.023
0.017
0.018
0.013
0.018
0.010
0.005
0.013
0.010
0.005
0.012
0.012
0.011
0.013
0.002
0.003
0.010
0.005
0.078
0.012
0.025
0.014
0.015
0.008
0.014
0.028
0.021
0.014
0.018
0.020
0.000
0.025
0.016
0.005
0.003
0.011
0.009
0.030
0.000
0.016
0.021
0.012
0.005
0.026
0.014
0.011
0.021
0.028
0.025
0.011
0.021
0.009
0.018
0.010
0.000
0.042
0.017
0.002
0.016
0.004
0.012
0.008
0.006
0.003
0.007
0.009
0.008
0.015
0.010
0.016
0.008
0.033
0.018
0.004
0.009
0.010
0.014
0.046
0.011
0.015
0.025
0.025
0.025
0.015
0.023
0.012
0.013
0.010
0.013
0.017
0.010
0.011
0.013
0.014
0.013
0.017
0.016
0.018
0.024
0.017
0.017
0.023
0.021
0.021
0.021
0.020
0.018
0.020
0.020
0.023
0.021
0.017
0.018
0.017
0.012
0.028
0.020
0.014
0.038
0.030
0.027
0.014
0.025
0.029
0.020
0.009
0.008
0.011
0.009
0.009
0.023
0.015
0.015
0.013
0.021
0.016
0.016
0.012
0.015
0.000
0.014
0.012
0.016
0.007
0.014
0.009
0.015
0.018
0.012
0.004
0.013
0.009
0.014
0.020
0.008
0.000
0.010
0.005
0.004
0.008
0.005
0.007
0.008
0.012
0.013
0.002
0.001
0.001
0.002
0.010
0.018
0.003
0.010
0.015
0.009
0.014
0.010
0.017
0.017
0.001
0.009
0.002
0.014
0.021
0.014
0.047
0.019
0.015
0.025
0.012
0.017
0.027
0.016
0.038
0.035
0.020
0.028
0.002
0.013
0.027
0.016
0.010
0.015
0.014
0.012
SD (g) 0.013 0.010 0.014 0.010 0.004 0.015 0.011 0.004 0.004 0.004 0.010 0.035 0.005 0.009 0.011 0.006 0.017 0.009 0.012 0.009 0.018 0.011 0.003 0.004 0.011 0.012 0.016 0.005 0.003 0.003 0.004 0.007 0.002 0.002 0.006 0.007 0.010 0.009 0.001 0.006 0.004 0.011 0.005 0.005 0.011 0.008 0.003 0.008 0.001 0.006 0.006 0.009 0.008 0.015 0.008 0.010 0.011 0.003
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3:
Feeding bout size (g)
cage 0102 cage 0106 cage 0202
0.045
0.043
0.032
0.018
0.018
0.038
0.024
0.011
0.033
0.017
0.026
0.025
0.036
0.024
0.008
0.002
0.019
0.026
0.036
0.017
0.018
0.012
0.008
0.024
0.015
0.011
0.019
0.013
0.001
0.013
0.010
0.008
0.015
0.001
0.004
0.028
0.018
0.018
0.024
0.012
0.019
0.009
0.009
0.007
0.025
0.015
0.028
0.036
0.028
0.009
0.021
0.028
0.031
0.046
0.020
0.001
0.027
0.011
0.000
0.024
0.024
0.003
0.016
0.022
0.008
0.012
0.000
0.000
0.023
0.014
0.011
0.010
0.013
0.012
0.015
0.055
0.010
0.031
0.044
0.025
0.028
0.009
0.012
0.032
0.027
0.031
0.012
0.006
0.015
0.011
0.014
0.011
0.020
0.010
0.009
0.010
0.023
0.017
0.018
0.017
0.032
0.017
0.005
0.033
0.024
0.038
0.022
0.025
0.032
0.019
0.032
0.030
0.018
0.020
0.013
0.008
0.014
0.025
0.015
0.011
0.015
0.010
0.023
0.014
0.006
0.004
0.007
0.013
0.015
0.013
0.011
0.007
0.020
0.001
0.022
0.008
0.030
0.022
0.004
0.009
0.007
0.011
0.019
0.020
0.000
0.011
0.005
0.007
0.007
0.006
0.002
0.005
0.013
0.000
0.011
0.009
0.013
0.023
0.018
0.014
0.023
0.010
0.033
0.008
0.041
0.019
0.023
0.022
0.017
0.008
0.021
0.006
0.015
0.010
HF + 0.03 % PFBS
Average feeding
cage 0206 bout size (g)
0.041
0.040
0.010
0.021
0.014
0.021
0.015
0.021
0.015
0.021
0.020
0.017
0.020
0.023
0.008
0.013
0.011
0.014
0.007
0.009
0.012
0.011
0.005
0.010
0.003
0.016
0.015
0.014
0.020
0.015
0.027
0.027
0.012
0.018
0.029
0.034
0.021
0.017
0.009
0.011
0.029
0.018
0.009
0.013
0.008
0.008
0.018
0.013
0.012
0.013
0.032
0.032
0.016
0.028
0.020
0.018
0.009
0.020
0.003
0.009
0.012
0.014
0.010
0.010
0.016
0.019
0.022
0.022
0.011
0.018
0.024
0.027
0.024
0.027
0.023
0.023
0.000
0.009
0.026
0.019
0.008
0.014
0.017
0.010
0.010
0.011
0.002
0.008
0.012
0.014
0.011
0.018
0.002
0.006
0.010
0.015
0.001
0.004
0.014
0.009
0.006
0.007
0.002
0.006
0.022
0.019
0.014
0.015
0.019
0.025
0.032
0.024
0.017
0.016
0.013
0.011
SD (g) 0.006 0.012 0.010 0.006 0.012 0.010 0.009 0.008 0.004 0.006 0.003 0.012 0.009 0.004 0.009 0.009 0.009 0.008 0.011 0.010 0.011 0.006 0.011 0.004 0.001 0.018 0.012 0.010 0.011 0.005 0.004 0.001 0.003 0.007 0.013 0.007 0.006 0.005 0.006 0.007 0.007 0.006 0.004 0.005 0.010 0.010 0.003 0.005 0.005 0.004 0.005 0.005 0.005 0.006 0.015 0.006 0.006 0.004
Page A32 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 4: HF + 0.006 % PFHS
Feeding bout size (g)
Average feeding
cage 0103 cage 0107 cage 0203 cage 0207 bout size (g)
0.043
0.050
0.060
0.066
0.055
0.019
0.023
0.037
0.037
0.029
0.009
0.023
0.014
0.025
0.018
0.022
0.014
0.011
0.009
0.014
0.007
0.007
0.025
0.021
0.015
0.010
0.024
0.006
0.004
0.011
0.020
0.000
0.015
0.020
0.014
0.010
0.006
0.011
0.001
0.007
0.013
0.005
0.006
0.019
0.011
0.013
0.012
0.007
0.012
0.011
0.011
0.027
0.021
0.005
0.016
0.021
0.040
0.008
0.023
0.023
0.018
0.013
0.014
0.008
0.013
0.016
0.029
0.010
0.021
0.019
0.015
0.009
0.025
0.013
0.016
0.019
0.001
0.024
0.024
0.017
0.009
0.019
0.024
0.030
0.021
0.036
0.063
0.056
0.041
0.049
0.033
0.030
0.044
0.048
0.039
0.024
0.020
0.049
0.049
0.036
0.014
0.012
0.036
0.017
0.020
0.005
0.009
0.019
0.025
0.015
0.010
0.017
0.005
0.000
0.008
0.012
0.000
0.018
0.010
0.010
0.009
0.012
0.013
0.008
0.011
0.028
0.032
0.015
0.007
0.021
0.019
0.040
0.020
0.030
0.027
0.017
0.000
0.012
0.019
0.012
0.022
0.044
0.021
0.011
0.025
0.009
0.015
0.007
0.011
0.011
0.007
0.016
0.010
0.008
0.010
0.012
0.017
0.030
0.019
0.020
0.013
0.035
0.011
0.010
0.017
0.028
0.007
0.029
0.017
0.020
0.037
0.026
0.037
0.017
0.029
0.033
0.015
0.035
0.022
0.026
0.040
0.027
0.027
0.060
0.039
0.030
0.039
0.032
0.026
0.032
0.022
0.025
0.023
0.036
0.027
0.013
0.004
0.001
0.020
0.010
0.008
0.008
0.021
0.016
0.013
0.006
0.002
0.006
0.014
0.007
0.014
0.007
0.018
0.006
0.011
0.004
0.005
0.017
0.007
0.008
0.011
0.008
0.015
0.018
0.013
0.014
0.012
0.014
0.011
0.013
0.017
0.002
0.009
0.009
0.009
0.008
0.015
0.000
0.014
0.009
0.011
0.009
0.022
0.009
0.013
0.006
0.000
0.001
0.008
0.004
0.001
0.010
0.017
0.008
0.009
0.014
0.010
0.013
0.013
0.013
0.006
0.014
0.015
0.013
0.012
0.034
0.013
0.011
0.024
0.021
0.041
0.008
0.035
0.023
0.027
0.034
0.015
0.030
0.025
0.026
0.037
0.016
0.019
0.036
0.027
0.017
0.014
0.025
0.013
0.017
SD (g) 0.010 0.009 0.008 0.006 0.009 0.009 0.009 0.005 0.007 0.003 0.010 0.013 0.004 0.008 0.007 0.011 0.009 0.013 0.009 0.016 0.011 0.009 0.007 0.007 0.002 0.012 0.010 0.009 0.014 0.003 0.004 0.008 0.012 0.010 0.010 0.009 0.016 0.005 0.006 0.009 0.006 0.005 0.006 0.006 0.004 0.002 0.006 0.007 0.006 0.004 0.007 0.002 0.004 0.011 0.015 0.008 0.011 0.005
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 6: HF + 0.003 % PFOS
Feeding bout size (g)
Average feeding
cage 0104 cage 0108 cage 0204 cage 0208 bout size (g)
0.040
0.017
0.037
0.035
0.032
0.026
0.000
0.043
0.025
0.024
0.020
0.010
0.018
0.018
0.017
0.024
0.036
0.025
0.018
0.026
0.022
0.015
0.027
0.013
0.019
0.030
0.009
0.027
0.014
0.020
0.027
0.003
0.014
0.014
0.015
0.008
0.019
0.019
0.008
0.014
0.010
0.001
0.000
0.018
0.007
0.026
0.013
0.009
0.002
0.013
0.014
0.013
0.007
0.012
0.012
0.011
0.008
0.010
0.013
0.011
0.015
0.017
0.011
0.013
0.014
0.019
0.010
0.004
0.014
0.012
0.024
0.013
0.006
0.015
0.015
0.018
0.010
0.023
0.033
0.021
0.013
0.031
0.015
0.033
0.023
0.050
0.013
0.049
0.024
0.034
0.020
0.046
0.029
0.033
0.032
0.027
0.016
0.031
0.011
0.021
0.013
0.006
0.035
0.024
0.020
0.014
0.003
0.023
0.011
0.013
0.010
0.016
0.017
0.012
0.014
0.011
0.012
0.036
0.019
0.020
0.011
0.004
0.004
0.002
0.005
0.039
0.023
0.022
0.023
0.027
0.029
0.007
0.014
0.022
0.018
0.024
0.013
0.012
0.026
0.019
0.023
0.004
0.013
0.014
0.014
0.018
0.016
0.017
0.002
0.013
0.013
0.003
0.011
0.012
0.010
0.020
0.020
0.012
0.013
0.016
0.013
0.010
0.015
0.015
0.013
0.066
0.010
0.020
0.018
0.029
0.026
0.016
0.019
0.012
0.018
0.031
0.029
0.032
0.019
0.028
0.027
0.029
0.031
0.027
0.029
0.021
0.019
0.029
0.023
0.023
0.012
0.014
0.017
0.014
0.014
0.014
0.012
0.016
0.026
0.017
0.019
0.005
0.012
0.005
0.010
0.015
0.006
0.005
0.011
0.009
0.015
0.014
0.014
0.001
0.011
0.014
0.015
0.001
0.006
0.009
0.023
0.010
0.014
0.013
0.015
0.012
0.013
0.014
0.010
0.012
0.016
0.001
0.021
0.008
0.012
0.015
0.017
0.005
0.009
0.012
0.010
0.002
0.005
0.008
0.006
0.012
0.008
0.007
0.001
0.007
0.011
0.003
0.009
0.000
0.006
0.012
0.014
0.006
0.007
0.010
0.009
0.014
0.027
0.003
0.013
0.022
0.008
0.011
0.020
0.015
0.028
0.028
0.021
0.087
0.041
0.027
0.021
0.019
0.015
0.021
0.020
0.031
0.036
0.030
0.029
0.007
0.016
0.013
0.016
0.013
SD (g) 0.010 0.018 0.004 0.008 0.006 0.010 0.010 0.006 0.008 0.010 0.003 0.002 0.003 0.006 0.007 0.010 0.010 0.018 0.011 0.009 0.013 0.008 0.003 0.012 0.004 0.008 0.010 0.007 0.008 0.008 0.005 0.004 0.002 0.025 0.006 0.006 0.002 0.004 0.002 0.006 0.007 0.005 0.007 0.007 0.006 0.002 0.009 0.006 0.004 0.005 0.005 0.004 0.010 0.007 0.031 0.005 0.007 0.004
Page A33 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p 4 4 0.886 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.200 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.057 4 4 0.686 4 4 0.029 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.343 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.200 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.460 4 4 0.343 4 4 0.200 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686
Feeding bout size; p-values Mann Whitney U-test group 1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6 nn p nn p nn p nn p nn p 4 4 0.114 4 4 0.486 4 4 0.057 4 4 0.200 4 4 0.029 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.200 4 4 0.114 4 4 0.486 4 4 0.057 4 4 1.000 4 4 0.200 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.114 4 4 0.343 4 4 0.686 4 4 0.686 4 4 0.200 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.029 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.886 4 4 0.486 4 4 0.486 4 4 0.200 4 4 0.486 4 4 1.000 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.200 4 4 0.343 4 4 0.200 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.886 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.114 4 4 0.200 4 4 0.486 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.029 4 4 0.114 4 4 0.114 4 4 1.000 4 4 0.343 4 4 0.114 4 4 0.486 4 4 0.029 4 4 0.114 4 4 0.343 4 4 0.200 4 4 0.486 4 4 0.057 4 4 0.114 4 4 0.343 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.057 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.686 4 4 0.114 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.029 4 4 0.114 4 4 0.486 4 4 0.200 4 4 0.343 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.686 4 4 0.860 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.029 4 4 0.200 4 4 0.343 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.029 4 4 0.029 4 4 0.886 4 4 0.343 4 4 0.029 4 4 0.343 4 4 0.029 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.114 4 4 1.000 4 4 0.200 4 4 0.886 4 4 0.114 4 4 0.343 4 4 0.140 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.343 4 4 1.000 4 4 0.686 4 4 0.057 4 4 0.686 4 4 0.057 4 4 0.886 4 4 0.057 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.200 4 4 0.029 4 4 0.486 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.200 4 4 0.343 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.343 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.343 4 4 0.343 4 4 0.486 4 4 0.029 4 4 0.057 4 4 0.114 4 4 0.486 4 4 0.114 4 4 0.114 4 4 0.486 4 4 0.200 4 4 1.000 4 4 0.343 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.686 4 4 1.000 4 4 1.000 4 4 0.057 4 4 0.343 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.114 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.057 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.343 4 4 0.057 4 4 0.886 4 4 0.200 4 4 0.686 4 4 0.114 4 4 0.343 4 4 0.343
p<0.05
Page A34 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Drink intake measurements
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1: HF control
Accumulated drink intake (ml)
Average acc. SD
cage 0101 cage 0105 cage 0201 cage 0205 drink intake (ml) (ml)
0.205
0.065
0.129
0.230
0.16
0.08
0.720
0.337
0.591
0.580
0.56
0.16
0.946
0.658
0.782
0.846
0.81
0.12
1.053
0.791
0.784
1.083
0.93
0.16
1.209
0.845
0.805
1.297
1.04
0.25
1.284
1.166
0.936
1.334
1.18
0.18
1.375
1.292
1.208
1.481
1.34
0.12
1.602
1.326
1.325
1.610
1.47
0.16
1.716
1.448
1.348
1.624
1.53
0.17
1.799
1.479
1.373
1.702
1.59
0.20
1.836
1.499
1.382
1.806
1.63
0.22
1.898
1.530
1.463
1.826
1.68
0.21
1.981
1.530
1.564
1.863
1.73
0.22
2.002
1.530
1.576
1.891
1.75
0.23
2.049
1.778
1.610
1.976
1.85
0.20
2.213
1.870
1.610
2.024
1.93
0.25
2.280
2.047
1.796
2.024
2.04
0.20
2.465
2.264
1.927
2.098
2.19
0.23
2.714
2.349
2.268
2.291
2.41
0.21
3.045
2.480
2.638
2.436
2.65
0.28
3.250
2.581
2.921
2.691
2.86
0.30
3.375
2.669
3.022
2.949
3.00
0.29
3.523
2.720
3.227
2.967
3.11
0.34
3.640
2.863
3.434
3.036
3.24
0.36
3.734
3.104
3.738
3.252
3.46
0.33
3.796
3.281
3.818
3.353
3.56
0.28
4.053
3.602
4.089
3.434
3.79
0.33
4.358
3.893
4.292
3.680
4.06
0.32
4.508
4.029
4.460
3.765
4.19
0.36
4.524
4.072
4.508
3.832
4.23
0.34
4.654
4.114
4.517
3.885
4.29
0.36
4.716
4.201
4.683
4.004
4.40
0.35
4.784
4.325
4.812
4.170
4.52
0.32
4.966
4.510
4.908
4.342
4.68
0.30
5.096
4.661
5.072
4.499
4.83
0.30
5.140
4.925
5.175
4.538
4.94
0.29
5.200
5.018
5.251
4.664
5.03
0.27
5.343
5.110
5.469
4.848
5.19
0.27
5.465
5.280
5.699
4.922
5.34
0.33
5.530
5.423
5.895
5.037
5.47
0.35
5.694
5.540
6.008
5.175
5.60
0.35
5.892
5.799
6.279
5.348
5.83
0.38
6.146
5.984
6.385
5.428
5.99
0.41
6.297
6.086
6.578
5.481
6.11
0.47
6.399
6.137
6.760
5.557
6.21
0.51
6.604
6.372
6.937
5.612
6.38
0.56
6.604
6.499
7.038
5.653
6.45
0.58
6.695
6.511
7.075
5.773
6.51
0.55
6.796
6.511
7.222
5.865
6.60
0.57
6.812
6.547
7.270
5.865
6.62
0.59
6.812
6.562
7.447
5.900
6.68
0.64
6.812
6.800
7.544
6.056
6.80
0.61
6.942
6.848
7.694
6.148
6.91
0.63
7.192
7.031
7.822
6.297
7.09
0.63
7.311
7.293
7.852
6.387
7.21
0.61
7.535
7.560
8.022
6.608
7.43
0.59
7.639
7.701
8.317
6.845
7.63
0.60
7.875
7.752
8.349
7.043
7.75
0.54
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
Accumulated drink intake (ml)
Average acc. SD
cage 0102 cage 0106 cage 0202 cage 0206 drink intake (ml) (ml)
0.125
0.077
0.074
0.092
0.09
0.02
0.387
0.392
0.569
0.388
0.43
0.09
0.699
0.496
0.967
0.560
0.68
0.21
0.883
0.510
1.102
0.676
0.79
0.26
0.912
0.691
1.253
0.864
0.93
0.24
0.994
0.800
1.449
1.024
1.07
0.27
1.176
0.824
1.634
1.276
1.23
0.33
1.318
0.834
1.800
1.400
1.34
0.40
1.462
0.848
1.850
1.400
1.39
0.41
1.565
0.861
2.153
1.450
1.51
0.53
1.592
0.872
2.254
1.500
1.55
0.57
1.600
0.880
2.311
1.500
1.57
0.59
1.659
0.880
2.578
1.548
1.67
0.70
1.730
0.923
2.743
1.616
1.75
0.75
1.768
1.003
2.804
1.780
1.84
0.74
1.837
1.013
3.065
1.842
1.94
0.85
1.898
1.024
3.283
1.988
2.05
0.93
2.045
1.078
3.512
2.146
2.20
1.00
2.210
1.179
3.904
2.280
2.39
1.13
2.357
1.194
4.171
2.418
2.53
1.23
2.539
1.205
4.361
2.606
2.68
1.29
2.710
1.216
4.558
2.764
2.81
1.37
2.912
1.232
4.669
2.906
2.93
1.40
2.968
1.245
4.869
2.988
3.02
1.48
3.101
1.302
4.941
3.026
3.09
1.49
3.251
1.360
5.004
3.248
3.22
1.49
3.547
1.386
5.263
3.424
3.41
1.59
3.787
1.453
5.465
3.520
3.56
1.65
3.955
1.557
5.645
3.520
3.67
1.68
4.046
1.664
5.702
3.590
3.75
1.66
4.059
1.725
5.731
3.632
3.79
1.65
4.130
1.776
5.814
3.722
3.86
1.66
4.232
1.864
6.048
3.788
3.98
1.72
4.293
1.925
6.185
3.874
4.07
1.75
4.387
2.098
6.361
4.048
4.22
1.75
4.488
2.166
6.577
4.100
4.33
1.81
4.763
2.238
6.869
4.252
4.53
1.90
5.006
2.384
6.988
4.420
4.70
1.89
5.194
2.560
7.207
4.608
4.89
1.91
5.344
2.587
7.337
4.804
5.02
1.95
5.506
2.672
7.529
5.032
5.18
1.99
5.597
2.782
7.729
5.188
5.32
2.03
5.693
2.838
7.884
5.352
5.44
2.07
5.742
2.869
8.100
5.420
5.53
2.14
5.842
2.960
8.217
5.510
5.63
2.15
6.122
3.056
8.334
5.646
5.79
2.17
6.157
3.131
8.496
5.700
5.87
2.20
6.176
3.146
8.674
5.836
5.96
2.26
6.198
3.157
8.726
5.900
6.00
2.28
6.213
3.168
8.748
5.946
6.02
2.28
6.235
3.224
8.748
6.000
6.05
2.26
6.250
3.272
8.748
6.000
6.07
2.24
6.301
3.341
8.975
6.072
6.17
2.30
6.358
3.434
9.104
6.260
6.29
2.32
6.584
3.462
9.446
6.362
6.46
2.44
6.795
3.486
9.756
6.532
6.64
2.56
6.912
3.550
9.992
6.730
6.80
2.63
6.998
3.640 10.177 6.890
6.93
2.67
Page A35 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Metabolic cages group 4: HF + 0.006 % PFHS
Time
Accumulated drink intake (ml)
Average acc.
SD
(hr) cage 0103 cage 0107 cage 0203 cage 0207 drink intake (ml) (ml)
6.1
0.131
0.362
0.214
0.306
0.25
0.10
7.4
0.361
0.782
0.880
0.849
0.72
0.24
8.6
0.479
0.996
1.327
1.135
0.98
0.36
9.9
0.574
1.207
1.512
1.254
1.14
0.40
11.1
0.612
1.207
1.718
1.395
1.23
0.46
12.4
0.669
1.248
1.961
1.518
1.35
0.54
13.6
0.768
1.343
1.995
1.558
1.42
0.51
14.9
0.819
1.370
2.085
1.606
1.47
0.53
16.1
0.982
1.377
2.184
1.624
1.54
0.50
17.4
1.113
1.377
2.184
1.694
1.59
0.46
18.6
1.121
1.550
2.297
1.729
1.67
0.49
19.9
1.159
1.758
2.310
1.815
1.76
0.47
21.1
1.290
1.880
2.329
1.971
1.87
0.43
22.4
1.463
1.938
2.520
2.024
1.99
0.43
23.6
1.516
1.955
2.545
2.024
2.01
0.42
24.9
1.592
1.955
2.747
2.081
2.09
0.48
26.1
1.615
1.955
2.982
2.152
2.18
0.58
27.4
1.685
2.072
3.137
2.429
2.33
0.62
28.6
2.048
2.708
3.620
2.974
2.84
0.65
29.9
2.329
2.948
4.053
3.518
3.21
0.74
31.1
2.453
3.074
4.574
3.846
3.49
0.92
32.4
2.512
3.186
4.893
4.052
3.66
1.04
33.6
2.546
3.349
4.998
4.092
3.75
1.05
34.9
2.595
3.451
4.998
4.145
3.80
1.02
36.1
2.645
3.461
5.093
4.312
3.88
1.06
37.4
2.683
3.667
5.187
4.312
3.96
1.06
38.6
2.831
3.856
5.271
4.396
4.09
1.02
39.9
2.945
3.910
5.414
4.576
4.21
1.04
41.1
3.027
4.175
5.527
4.708
4.36
1.05
42.4
3.173
4.352
5.565
4.717
4.45
0.99
43.6
3.192
4.352
5.649
4.752
4.49
1.02
44.9
3.211
4.423
5.712
4.759
4.53
1.03
46.1
3.276
4.498
5.943
4.888
4.65
1.10
47.4
3.416
4.651
6.203
4.950
4.81
1.14
48.6
3.585
4.763
6.422
4.950
4.93
1.16
49.9
3.753
4.859
6.766
5.025
5.10
1.25
51.1
4.051
5.005
7.046
5.350
5.36
1.25
52.4
4.366
5.263
7.388
5.870
5.72
1.27
53.6
4.579
5.632
7.642
6.395
6.06
1.29
54.9
4.579
5.794
7.854
6.743
6.24
1.39
56.1
4.579
5.868
7.892
7.018
6.34
1.44
57.4
4.579
5.930
8.100
7.227
6.46
1.54
58.6
4.699
6.011
8.245
7.454
6.60
1.57
59.9
4.803
6.042
8.392
7.608
6.71
1.60
61.1
4.872
6.106
8.610
7.726
6.83
1.67
62.4
4.940
6.188
8.736
7.806
6.92
1.69
63.6
4.978
6.297
8.736
7.854
6.97
1.67
64.9
5.043
6.351
8.736
7.920
7.01
1.64
66.1
5.088
6.450
8.763
8.004
7.08
1.64
67.4
5.111
6.460
8.904
8.074
7.14
1.69
68.6
5.111
6.504
8.988
8.140
7.19
1.73
69.9
5.172
6.542
9.114
8.175
7.25
1.75
71.1
5.187
6.664
9.284
8.228
7.34
1.79
72.4
5.324
6.725
9.303
8.391
7.44
1.77
73.6
5.596
6.732
9.574
8.532
7.61
1.78
74.9
5.917
6.773
9.811
8.708
7.80
1.78
76.1
6.236
6.943 10.019 9.082
8.07
1.78
77.4
6.335
7.133 10.185 9.297
8.24
1.80
Metabolic cages group 6: HF + 0.003 % PFOS
Time
Accumulated drink intake (ml)
Average acc.
SD
(hr) cage 0104 cage 0108 cage 0204 cage 0208 drink intake (ml) (ml)
6.1
0.134
0.240
0.151
0.202
0.18
0.05
7.4
0.711
0.288
0.724
0.470
0.55
0.21
8.6
1.078
0.328
1.159
0.616
0.80
0.39
9.9
1.282
0.563
1.269
0.716
0.96
0.37
11.1
1.576
0.813
1.505
0.840
1.18
0.41
12.4
1.877
0.883
1.667
0.976
1.35
0.50
13.6
2.179
0.896
1.818
1.062
1.49
0.61
14.9
2.394
0.941
1.957
1.100
1.60
0.69
16.1
2.567
0.992
2.016
1.216
1.70
0.73
17.4
2.669
1.085
2.016
1.280
1.76
0.73
18.6
2.820
1.146
2.016
1.288
1.82
0.77
19.9
2.948
1.253
2.016
1.360
1.89
0.78
21.1
2.992
1.264
2.016
1.422
1.92
0.78
22.4
2.992
1.278
2.016
1.446
1.93
0.77
23.6
3.012
1.312
2.016
1.560
1.98
0.75
24.9
3.135
1.312
2.088
1.576
2.03
0.81
26.1
3.196
1.338
2.160
1.630
2.08
0.82
27.4
3.344
1.506
2.198
1.794
2.21
0.81
28.6
3.611
1.768
2.362
2.022
2.44
0.82
29.9
3.822
2.093
2.907
2.198
2.75
0.80
31.1
4.039
2.198
3.308
2.390
2.98
0.85
32.4
4.097
2.317
3.438
2.534
3.10
0.83
33.6
4.097
2.394
3.620
2.664
3.19
0.80
34.9
4.097
2.557
3.978
2.808
3.36
0.79
36.1
4.097
2.642
4.104
2.940
3.45
0.77
37.4
4.148
2.720
4.111
3.000
3.49
0.74
38.6
4.423
2.774
4.176
3.134
3.63
0.80
39.9
4.648
2.832
4.363
3.342
3.80
0.85
41.1
4.784
2.864
4.437
3.468
3.89
0.88
42.4
4.944
2.902
4.464
3.480
3.95
0.93
43.6
5.117
3.024
4.529
3.494
4.04
0.95
44.9
5.151
3.024
4.698
3.506
4.09
1.00
46.1
5.156
3.046
4.698
3.550
4.11
0.98
47.4
5.202
3.178
4.698
3.636
4.18
0.93
48.6
5.263
3.205
4.698
3.732
4.22
0.93
49.9
5.535
3.334
4.806
3.858
4.38
0.98
51.1
5.879
3.624
5.123
3.974
4.65
1.04
52.4
5.964
3.898
5.377
4.148
4.85
0.99
53.6
6.137
4.242
5.555
4.378
5.08
0.92
54.9
6.392
4.346
5.767
4.560
5.27
0.98
56.1
6.681
4.536
5.994
4.676
5.47
1.04
57.4
6.871
4.608
6.118
4.778
5.59
1.09
58.6
6.989
4.707
6.311
4.958
5.74
1.09
59.9
7.342
4.891
6.390
5.020
5.91
1.17
61.1
7.431
4.912
6.604
5.212
6.04
1.18
62.4
7.497
5.022
6.772
5.346
6.16
1.17
63.6
7.616
5.152
6.804
5.452
6.26
1.16
64.9
7.616
5.168
6.804
5.516
6.28
1.14
66.1
7.616
5.216
6.804
5.540
6.29
1.12
67.4
7.659
5.222
6.804
5.540
6.31
1.13
68.6
7.701
5.232
6.804
5.540
6.32
1.15
69.9
7.701
5.290
6.804
5.568
6.34
1.12
71.1
7.701
5.347
6.804
5.630
6.37
1.09
72.4
7.701
5.408
6.804
5.676
6.40
1.06
73.6
7.813
5.504
6.944
5.824
6.52
1.06
74.9
8.050
5.678
7.175
5.952
6.71
1.10
76.1
8.109
5.987
7.547
6.098
6.94
1.06
77.4
8.109
6.242
7.686
6.304
7.09
0.95
Page A36 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p 4 4 0.343 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.860 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.343
Accumulated drink intake; p-values Mann Whitney U-test
group 1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6
nn p nn p nn p nn p nn p
4 4 0.343 4 4 0.686 4 4 0.029 4 4 0.029 4 4 0.343
4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.200
4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.686
4 4 0.343 4 4 1.000 4 4 0.200 4 4 0.486 4 4 0.886
4 4 0.486 4 4 0.686 4 4 0.486 4 4 0.686 4 4 1.000
4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.686 4 4 1.000
4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.886
4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.886
4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.886
4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 1.000
4 4 1.000 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.886
4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.886
4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.886 4 4 1.000
4 4 0.486 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.686
4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.886
4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.886
4 4 1.000 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000
4 4 1.000 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886
4 4 0.343 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.343
4 4 0.486 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.343
4 4 0.486 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.486 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.486 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.486 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.486
p<0.05
4 4 0.686 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.686 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.686 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.686 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.686
4 4 0.686 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.686
4 4 0.686 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.686
4 4 0.686 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.686
4 4 0.686 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.686
4 4 0.686 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.686
4 4 1.000 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.886 4 4 0.486 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.886 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.686 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.486 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.200
4 4 0.486 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.200
4 4 0.486 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.486 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.686 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.486 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.486 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.686
4 4 0.886 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.486
4 4 0.886 4 4 0.486 4 4 0.686 4 4 1.000 4 4 0.486
4 4 0.886 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.343
4 4 0.886 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.343
Page A37 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
D4. X activity measurements
Metabolic cages group 1: HF control
X activity per interval
cage 0101 cage 0105 cage 0201 cage 0205
100.7
88.8
134.7
99.5
63.9
227.7
123.3
73.9
67.5
124.2
57.2
69.0
76.1
238.3
182.4
62.8
90.7
71.1
72.9
173.1
88.6
74.3
128.0
80.4
69.1
109.8
54.7
35.5
72.2
28.9
67.7
67.7
14.3
92.9
70.7
46.6
112.4
64.9
119.1
63.5
144.9
81.0
41.1
47.5
74.2
81.1
92.5
166.7
44.1
99.0
49.7
29.3
47.4
71.0
121.8
94.7
88.3
91.9
108.7
57.4
424.8
330.7
375.7
615.8
133.7
276.6
421.4
814.9
331.4
346.2
385.6
228.3
121.9
221.1
257.7
488.8
143.4
139.3
121.1
158.0
133.1
229.4
213.0
217.7
74.2
181.4
129.7
250.7
54.6
182.5
199.9
94.7
172.7
166.9
158.2
119.2
84.7
128.2
53.6
109.3
101.7
122.5
136.4
118.5
53.4
25.2
67.8
54.2
33.9
49.9
56.4
55.9
84.9
47.7
44.7
23.0
28.9
79.6
74.6
119.0
64.8
34.9
59.5
96.2
74.9
64.0
65.7
38.0
69.8
101.4
87.1
72.6
90.4
52.7
46.1
71.2
373.3
279.0
284.9
313.7
337.7
147.0
370.8
371.0
361.6
270.6
276.3
229.2
161.5
365.6
199.2
227.9
164.7
143.5
156.8
202.0
119.6
206.4
153.3
185.1
76.2
117.9
136.6
57.6
82.3
180.9
143.9
106.5
96.9
97.8
154.2
234.6
173.9
144.2
96.5
86.3
90.2
68.3
85.5
90.4
71.4
80.6
87.9
29.3
49.0
185.5
93.7
91.4
94.4
67.4
115.8
76.0
36.2
14.6
23.2
57.4
91.6
118.8
97.4
31.4
42.5
77.5
37.2
106.7
50.0
112.7
53.7
40.3
94.0
43.9
83.9
120.3
186.7
126.5
215.6
235.7
380.3
349.8
374.6
551.9
411.4
223.4
274.0
372.4
246.9
230.0
248.0
185.3
156.4
360.5
270.5
260.9
Average X activity
105.9 122.2 79.5 139.9 102.0 92.8 67.3 59.1 56.1 90.0 78.6 103.6 55.5 83.7 86.6 436.8 411.7 322.9 272.4 140.5 198.3 159.0 132.9 154.3 94.0 119.8 50.2 49.0 50.1 75.5 63.9 60.7 82.7 65.1 312.7 306.6 284.4 238.6 166.8 166.1 97.1 128.4 145.9 125.2 83.6 67.3 104.9 88.4 32.9 84.8 66.0 64.2 85.5 191.1 414.2 320.3 227.6 262.1
SD
19.9 75.0 30.3 84.7 48.3 24.2 31.5 20.3 33.7 29.9 47.5 42.7 30.2 31.9 21.4 125.4 293.4 67.1 155.3 15.2 44.0 75.2 69.6 24.1 32.3 14.3 17.9 10.5 25.7 36.9 25.2 15.8 14.6 19.9 43.1 107.6 55.6 89.0 25.1 37.9 36.5 43.2 64.9 41.1 10.4 26.2 57.5 21.5 18.6 37.5 32.5 32.8 31.7 47.5 92.8 86.7 29.3 83.5
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
X activity per interval
Average
cage 0102 cage 0106 cage 0202 cage 0206 X activity
92.2
254.5
88.7
185.0
155.1
92.7
238.4
92.6
375.7
199.9
79.1
99.1
110.4
111.4
100.0
131.6
82.0
45.9
221.7
120.3
134.8
124.3
259.8
60.1
144.8
54.7
138.5
174.2
39.8
101.8
134.4
53.3
68.6
182.7
109.8
59.0
82.5
103.6
47.8
73.2
122.4
105.6
146.3
18.4
98.2
84.7
29.6
245.5
93.8
113.4
158.6
172.4
76.4
41.6
112.3
21.0
39.6
50.1
14.5
31.3
93.0
113.8
169.7
118.6
123.8
111.5
160.0
89.9
126.0
121.9
158.6
55.4
169.7
72.4
114.0
454.8
579.9
431.8
455.2
480.4
432.8
621.4
618.1
933.4
651.4
277.4
351.9
422.6
616.8
417.2
325.6
48.8
191.6
130.5
174.1
262.1
373.9
151.6
282.2
267.5
95.4
52.6
238.0
216.4
150.6
127.8
215.5
260.5
148.1
188.0
99.6
135.3
127.1
112.9
118.7
199.2
141.3
217.7
267.4
206.4
135.8
276.8
172.6
73.8
164.8
109.4
98.3
200.9
103.7
128.1
58.6
61.8
132.5
28.6
70.4
61.9
88.5
155.1
26.9
83.1
60.3
164.0
35.5
11.7
67.9
47.7
32.8
87.4
223.3
97.8
69.2
57.8
71.1
68.3
66.6
131.2
97.6
173.2
60.2
115.6
88.0
78.5
143.7
50.2
90.1
120.8
63.9
104.5
30.4
79.9
361.3
203.4
323.6
485.2
343.4
527.6
382.4
442.4 1194.3
636.7
308.7
470.4
298.9
609.6
421.9
201.9
177.0
84.2
327.0
197.5
332.9
80.5
218.2
274.2
226.5
117.4
102.8
219.6
242.1
170.5
136.8
300.6
165.3
167.6
192.6
121.2
113.3
113.2
309.0
164.2
177.5
352.3
305.4
374.6
302.5
236.1
139.3
186.5
212.9
193.7
70.6
171.6
92.8
28.1
90.8
102.9
131.2
71.9
161.7
116.9
72.1
72.4
43.7
9.3
49.4
62.0
63.4
125.6
266.9
129.5
130.8
49.7
53.5
23.2
64.3
75.2
27.2
84.9
221.5
102.2
84.0
116.4
51.9
45.1
74.4
63.1
82.3
73.4
35.7
63.6
129.7
62.8
151.3
92.7
109.1
201.6
143.3
125.5
219.4
172.5
429.8
396.0
387.1
405.0
404.5
278.5
493.7
280.0
701.6
438.5
270.4
175.4
217.9
407.2
267.7
256.5
298.8
295.6
603.1
363.5
SD
79.9 135.9 15.0 76.2 83.5 64.9 60.0 24.9 55.7 92.5 63.4 16.4 32.6 29.4 58.5 67.2 207.6 145.7 116.7 91.3 90.6 61.2 15.8 52.1 85.1 48.8 44.0 54.2 67.1 86.8
6.0 48.1 39.2 40.8 116.1 376.5 147.8 100.1 108.0 70.6 73.4 96.6 88.2 41.5 60.2 38.4 29.9 96.3 46.3 83.4 32.8 20.2 39.2 45.1 18.4 202.5 100.8 160.9
Page A38 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 4: HF + 0.006 % PFHS
X activity per interval
Average
cage 0103 cage 0107 cage 0203 cage 0207 X activity
75.4
118.1
92.7
258.6
136.2
91.1
61.6
100.7
151.8
101.3
32.3
128.9
85.4
124.6
92.8
88.7
58.6
66.1
53.7
66.8
56.0
47.9
89.9
144.3
84.5
79.3
139.0
66.9
185.7
117.7
103.8
31.0
43.1
57.7
58.9
51.9
53.5
153.0
95.1
88.4
65.2
199.5
53.3
90.9
102.2
83.7
172.4
74.7
34.6
91.4
28.4
46.4
42.0
124.4
60.3
59.3
94.9
103.2
76.9
83.6
108.0
72.6
51.6
57.3
72.4
59.8
39.3
122.1
273.1
123.6
71.2
76.4
74.7
95.4
79.4
484.3
589.1
168.2
410.3
413.0
821.1
605.3
368.9
719.7
628.8
418.6
371.6
219.7
566.0
394.0
282.1
99.3
260.1
379.5
255.3
175.2
215.2
227.2
177.6
198.8
203.6
190.6
144.7
107.9
161.7
92.2
84.7
83.6
169.4
107.5
146.6
178.0
157.2
185.6
166.9
85.3
14.1
101.8
125.7
81.7
173.4
94.0
82.6
336.9
171.7
141.5
111.9
103.3
374.0
182.7
97.6
65.8
82.8
63.6
77.5
46.4
28.1
47.1
140.5
65.5
54.7
69.4
73.6
65.1
65.7
47.0
47.6
44.3
73.3
53.1
79.7
55.9
49.8
76.8
65.6
33.1
77.6
137.3
47.2
73.8
141.3
169.8
67.1
187.9
141.5
148.8
21.7
77.9
55.3
75.9
211.0
139.5
118.3
271.5
185.1
555.2
277.4
176.1
489.8
374.6
227.0
219.5
308.1
548.7
325.8
192.2
120.1
214.5
482.5
252.3
145.5
229.0
129.1
229.2
183.2
159.5
85.7
211.4
146.7
150.8
114.2
96.2
119.5
189.7
129.9
64.4
84.0
123.6
69.4
85.4
116.3
161.9
141.4
211.7
157.8
59.8
24.8
79.1
127.3
72.8
98.7
39.5
90.8
97.0
81.5
97.6
87.9
60.4
116.3
90.6
97.9
39.2
108.8
93.5
84.9
87.3
120.3
55.8
55.3
79.7
69.4
44.1
120.1
211.5
111.3
63.4
28.1
69.2
83.9
61.2
34.3
79.2
92.1
126.5
83.0
77.4
110.7
103.2
151.6
110.7
54.9
103.1
77.5
66.3
75.5
121.3
183.4
84.3
76.1
116.3
262.2
613.3
156.4
233.3
316.3
393.8
463.2
326.7
686.7
467.6
152.2
146.0
423.4
234.1
238.9
160.4
290.0
246.2
300.4
249.3
SD
83.5 37.6 44.8 15.5 43.8 55.2 31.9 47.5 66.7 58.1 43.4 19.6 25.4 105.7 10.9 178.9 194.4 142.7 116.2 26.3 43.9 41.5 18.1 48.0 117.3 128.6 15.9 50.8 8.1 13.6 14.9 46.2 53.2 53.8 69.9 177.7 153.9 158.7 53.4 51.6 41.1 26.8 40.5 42.8 28.2 23.3 31.1 30.9 73.9 23.7 38.1 30.8 20.6 48.9 203.0 156.3 129.4 63.7
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 6: HF + 0.003 % PFOS
X activity per interval
Average
cage 0104 cage 0108 cage 0204 cage 0208 X activity
152.1
223.2
114.3
130.4
155.0
136.3
217.5
107.7
211.5
168.3
37.5
132.4
78.6
35.1
70.9
120.1
164.6
61.9
77.3
106.0
165.9
124.0
96.1
64.6
112.7
64.9
56.9
76.3
42.0
60.0
99.3
51.5
54.9
136.8
85.6
66.3
168.5
118.2
51.8
101.2
35.2
74.0
67.3
72.4
62.2
29.9
94.3
55.5
23.5
50.8
75.5
152.4
123.9
48.4
100.1
94.7
30.6
41.5
67.2
58.5
61.8
90.8
77.4
96.6
81.7
41.2
176.7
52.9
68.4
84.8
154.0
98.7
119.9
115.8
122.1
248.5
353.9
134.8
128.7
216.5
432.4
353.1
703.1
283.1
442.9
225.6
313.2
437.8
367.4
336.0
250.2
403.1
203.5
248.2
276.3
130.8
117.3
152.3
164.3
141.2
194.5
243.2
125.1
138.7
175.4
136.1
54.8
78.9
156.4
106.6
138.1
137.0
183.6
133.4
148.0
250.1
184.4
140.2
133.4
177.0
164.8
39.0
140.4
63.1
101.8
111.8
88.9
133.9
108.8
110.9
81.0
99.0
41.5
98.2
79.9
64.7
12.4
52.8
123.7
63.4
29.8
48.0
55.2
50.8
46.0
36.2
141.9
24.8
28.7
57.9
73.6
35.9
147.9
112.9
92.6
75.9
45.5
63.5
32.1
54.3
42.3
63.0
57.3
78.0
60.2
38.4
84.7
46.8
106.0
69.0
140.0
152.0
217.4
78.4
147.0
405.1
292.8
232.2
226.6
289.2
131.5
332.3
228.9
252.9
236.4
212.6
410.7
208.7
235.1
266.8
114.7
310.1
107.5
227.4
189.9
136.0
161.8
93.3
83.1
118.6
198.1
73.7
158.3
108.8
134.7
89.1
134.6
91.5
166.0
120.3
136.5
126.4
110.9
175.2
137.3
180.5
105.8
129.4
141.3
139.3
85.9
71.5
90.4
116.7
91.1
56.8
151.5
70.7
107.5
96.6
74.8
40.4
70.0
67.5
63.2
42.2
130.3
32.1
72.8
69.4
26.7
24.3
85.4
91.4
57.0
74.5
80.0
53.0
61.2
67.2
59.1
27.3
68.7
34.4
47.4
57.3
101.8
40.5
102.7
75.6
55.0
80.3
57.9
55.6
62.2
106.5
43.9
112.9
98.0
90.3
193.8
308.6
110.3
109.8
180.6
256.2
142.5
208.6
290.9
224.6
131.1
305.9
223.4
367.3
256.9
203.7
407.8
178.4
337.2
281.8
SD
48.0 54.7 45.6 46.2 43.0 14.4 40.5 53.2 18.2 32.1 46.8 28.6 15.5 62.3 23.2 106.9 183.9 89.5 87.3 21.1 54.3 47.6 23.8 53.7 60.3 18.4 26.9 46.0 11.2 56.2 48.5 19.3 14.8 31.9 57.0 82.9 82.7 96.7 97.1 36.8 54.7 36.9 27.4 31.2 18.9 42.4 15.5 44.2 36.4 12.3 19.7 31.6 12.1 31.5 94.0 64.3 102.5 109.1
Page A39 of A172
Appendix to TNO-report of 3M-02
Final version
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.486 4 4 0.686 4 4 0.200 4 4 1.000 4 4 0.486 4 4 0.029 4 4 0.057 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.486 4 4 0.057 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.200 4 4 0.200 4 4 0.686 4 4 0.486 4 4 0.343 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.684 4 4 0.686 4 4 0.029 4 4 0.114 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.029 4 4 0.686 4 4 0.057 4 4 0.114 4 4 0.886 4 4 0.114 4 4 0.114 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.486
group 1 vs 4 nn p 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.200 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.200 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.029 4 4 0.200 4 4 0.486 4 4 0.886 4 4 0.057 4 4 0.486 4 4 0.686 4 4 0.114 4 4 0.686 4 4 0.343 4 4 0.343 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.029 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.114 4 4 0.343 4 4 0.200 4 4 1.000 4 4 0.686 4 4 0.057 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.057 4 4 0.343 4 4 0.200 4 4 0.686 4 4 1.000
X activity; p-values Mann Whitney U-test group 1 vs 6 group 3 vs 4 group 3 vs 6 nn p nn p nn p 4 4 0.114 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.343 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.057 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.343 4 4 0.686 4 4 0.200 4 4 0.029 4 4 0.200 4 4 0.343 4 4 0.057 4 4 0.057 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.057 4 4 0.886 4 4 1.000 4 4 0.057 4 4 0.886 4 4 0.029 4 4 0.686 4 4 0.886 4 4 0.200 4 4 1.000 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.486 4 4 0.200 4 4 1.000 4 4 0.343 4 4 0.114 4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.486 4 4 0.114 4 4 0.200 4 4 0.886 4 4 0.029 4 4 0.057 4 4 0.686 4 4 0.029 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.486 4 4 1.000 4 4 0.200 4 4 0.343 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.486 4 4 0.486 4 4 0.343 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.343 4 4 0.114 4 4 0.114 4 4 0.343 4 4 0.200 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.029 4 4 0.114 4 4 0.057 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.486 4 4 0.686 4 4 0.200 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.200 4 4 0.686 4 4 0.343 4 4 0.486 4 4 0.200 4 4 0.343 4 4 0.886 4 4 0.200 4 4 0.686 4 4 0.886 4 4 0.057 4 4 0.029 4 4 0.686 4 4 0.029 4 4 0.114 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.343 4 4 0.486 4 4 0.200 4 4 0.200 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.486 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.343 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.057 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.057 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.029 4 4 0.343 4 4 0.029 4 4 0.200 4 4 0.886 4 4 0.114 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.686
group 4 vs 6 nn p 4 4 0.486 4 4 0.114 4 4 0.886 4 4 0.200 4 4 0.343 4 4 0.057 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.200 4 4 0.486 4 4 0.886 4 4 0.029 4 4 0.114 4 4 0.200 4 4 0.686 4 4 0.886 4 4 0.029 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.029 4 4 0.343 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.057 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.057 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.114 4 4 0.486 4 4 0.057 4 4 0.886 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.200 4 4 0.114 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.029 4 4 1.000 4 4 0.686
04-12-2006
p<0.05
Page A40 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
D5. O2 consumption,CO2 production and respiratory exchange ratio measurements
O2 consumption
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1: HF control
VO2 (ml/h/kg mouse)
Average VO2 SD
cage 0101 cage 0105 cage 0201 cage 0205 (ml/h/kg mouse)
3055
3609
3572
3183
3355
278
2909
3769
3278
2987
3236
389
2786
3499
3070
3028
3096
296
3051
3540
3607
2913
3278
347
2741
3200
2943
3204
3022
224
2814
2966
3194
2903
2969
162
2636
3102
2744
2661
2786
216
2685
2756
2766
2705
2728
39
2399
2814
2770
2676
2665
186
2811
2699
2874
2756
2785
75
3115
2850
2610
2730
2826
216
2766
2917
2939
3098
2930
136
2602
2990
2628
2542
2691
203
2743
2708
2828
2892
2793
83
3001
2983
2979
2785
2937
102
3803
3693
3803
3939
3809
101
2959
3356
3539
3857
3428
375
3391
3518
3497
3027
3358
228
2921
3300
3385
3544
3287
264
2968
3266
3115
2992
3085
136
2981
3263
3316
3042
3150
164
2763
3180
3307
3199
3112
239
2742
3182
3401
2921
3062
290
3160
3090
3203
2842
3074
162
2844
3097
2702
2680
2831
192
2831
2938
3274
2716
2940
240
2671
2564
2826
2528
2647
134
2400
2696
2776
2658
2633
163
2690
2672
2630
2450
2611
110
2344
2796
2790
2828
2689
231
2542
2523
2642
2639
2587
63
2549
2679
2872
2558
2665
151
2757
2884
3000
2753
2849
118
2937
2860
2704
2758
2815
104
3585
3535
3750
3397
3566
146
3458
3277
3756
3348
3460
211
3320
3475
3528
3083
3352
199
2985
3469
3327
3112
3223
216
2933
3202
3213
3143
3123
130
3066
3289
3231
3090
3169
108
2657
3247
3191
2815
2977
287
2818
3230
3258
2922
3057
220
2822
2877
3274
3079
3013
206
2899
3156
3119
2652
2957
233
2683
2820
2794
2648
2736
84
2389
2882
2765
2368
2601
262
2451
2707
2939
2554
2663
212
2727
2568
2982
2495
2693
216
2399
2276
2392
2563
2407
118
2588
2697
2816
2448
2637
157
2392
2652
2564
2667
2569
126
2532
2656
2612
2506
2576
70
2612
2511
2816
2723
2666
132
3115
3156
3363
3306
3235
119
3315
3487
3910
3443
3539
258
3280
3320
3488
3288
3344
98
2917
3251
3373
2915
3114
234
2855
3337
3389
3106
3172
244
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
VO2 (ml/h/kg mouse)
Average VO2 SD
cage 0102 cage 0106 cage 0202 cage 0206 (ml/h/kg mouse)
3472
3369
3160
3514
3379
158
3395
3409
3368
3614
3446
113
3050
3203
3449
3293
3249
167
3172
2947
3117
3489
3181
226
3251
3125
3816
3179
3343
320
2926
3060
3395
2899
3070
228
3037
2695
3096
3070
2975
188
2788
2851
3097
2552
2822
224
2871
2875
3325
2576
2912
309
2799
2652
3518
2756
2931
396
3056
3007
3074
2662
2950
194
2601
2709
3117
2514
2735
267
2805
2795
3413
2879
2973
296
2750
2921
3103
2867
2910
147
3171
2587
3536
2788
3020
420
3833
3748
4154
3592
3832
237
3636
3978
4043
3820
3869
181
3312
3246
3549
3655
3441
193
3416
2692
3359
3071
3135
332
3230
3470
3388
3377
3366
100
2869
2582
3516
3131
3025
397
2856
2876
3552
3029
3078
325
2994
2690
3323
2895
2975
264
3079
2731
3477
3112
3100
305
2968
3042
3324
2751
3022
237
3173
2525
3366
2900
2991
364
2704
2513
3166
2538
2730
302
2718
2533
3179
2470
2725
320
2628
2616
3017
2540
2700
215
2607
2201
3282
3134
2806
496
2730
2354
3253
2736
2768
369
2797
2485
3350
2734
2842
365
2698
2627
3198
2680
2801
267
3046
2525
3342
2752
2916
355
3577
3124
3851
3637
3547
306
3845
3499
3852
3908
3776
187
3451
3441
3668
3515
3519
105
3181
2990
3312
3294
3194
148
3346
2800
3445
3165
3189
284
3086
2879
3554
3289
3202
289
3090
3022
3415
3186
3178
172
2863
2881
3403
3262
3102
272
3087
3127
3591
3313
3279
230
3056
2850
3467
3039
3103
260
2671
3145
3078
2635
2882
266
2444
2789
2892
2715
2710
191
2399
2700
2935
2380
2603
265
2342
2391
3212
2899
2711
418
2545
2295
2815
2392
2512
227
2404
2324
3006
2846
2645
332
2463
2660
2803
2507
2608
155
2455
2428
2845
2482
2553
196
2691
2326
3266
2798
2770
387
3248
2864
3489
3160
3190
258
3502
3251
4078
3573
3601
346
3217
3329
3610
3467
3406
170
3102
2931
3421
3432
3222
247
3005
2951
3571
3348
3219
293
Page A41 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 4: HF + 0.006 % PFHS
VO2 (ml/h/kg mouse)
Average VO2 SD
cage 0103 cage 0107 cage 0203 cage 0207 (ml/h/kg mouse)
3575
3707
3794
3663
3685
91
3438
3450
3743
3572
3551
141
3276
3564
3720
3214
3443
240
3298
3287
3705
3019
3327
283
3219
3026
3779
3239
3316
323
3263
3409
3672
3345
3422
177
3253
2782
3223
2912
3043
233
3148
2910
3420
2872
3088
253
3131
3357
3169
2875
3133
198
3100
3284
3191
2771
3086
224
3016
2897
2977
2999
2972
53
3076
3165
3223
2870
3084
155
3085
2998
2948
2834
2966
105
3053
2966
3152
3341
3128
161
3230
3150
3288
3059
3182
99
4037
4423
3777
3640
3969
345
4262
4267
4205
4105
4210
75
3794
3878
3828
3747
3812
55
3514
3369
3957
3750
3648
260
3148
3529
3640
3236
3388
234
3395
3172
3650
3219
3359
216
3149
2853
3984
3250
3309
480
3169
3211
3921
3297
3399
352
2970
2429
3604
3069
3018
481
3160
2889
3350
3309
3177
209
3016
3030
3543
3283
3218
249
2941
2788
3214
2655
2899
240
2850
2683
3085
2885
2876
165
2941
2931
3210
2530
2903
280
2907
2730
2981
2725
2836
128
2942
2681
2965
2754
2835
140
2796
2997
3440
2692
2981
331
3066
3271
3046
3180
3141
105
3205
2865
3259
2689
3005
273
3687
3511
3496
3092
3446
252
4214
3964
4094
3677
3987
230
3775
3687
4344
3700
3876
314
3674
3386
3911
3750
3680
220
3475
3563
3848
3319
3551
222
3342
2927
3707
3329
3326
319
3292
3075
3560
3360
3322
200
3037
2999
3737
3049
3206
355
3252
3141
3706
3142
3310
269
2949
2581
3450
2942
2980
357
2998
2658
3240
2765
2915
259
3028
2847
3009
2797
2920
116
2891
2517
3312
2674
2848
345
2898
2895
3049
2635
2869
172
2599
2387
3169
3032
2797
366
2763
2486
2988
2671
2727
209
2667
2703
3097
2657
2781
211
2847
2910
3004
2767
2882
100
2862
2964
3025
2614
2866
181
3237
3292
3296
2919
3186
180
3789
4002
3796
3425
3753
240
3950
3705
4281
3810
3937
251
3367
2971
4117
3470
3481
475
3409
3211
3938
3360
3480
317
Metabolic cages group 6: HF + 0.003 % PFOS
Time
VO2 (ml/h/kg mouse)
Average VO2
SD
(hr) cage 0104 cage 0108 cage 0204 cage 0208 (ml/h/kg mouse)
6.1
3936
4321
3970
3628
3963
283
7.4
3779
3980
3795
3648
3800
136
8.6
3334
3807
3674
3379
3549
229
9.9
3695
3783
3579
3441
3624
148
11.1
3866
3646
3646
3141
3575
307
12.4
3633
3225
3349
3124
3333
220
13.6
3766
3107
3222
3167
3315
304
14.9
3592
3471
3249
3007
3330
258
16.1
3335
2903
3089
3039
3091
180
17.4
3212
3171
2998
2777
3040
198
18.6
3726
3504
3191
2894
3329
364
19.9
3676
2963
2911
3007
3139
360
21.1
3305
3099
2949
3046
3100
150
22.4
3297
3319
2980
3014
3152
180
23.6
3731
3204
3078
3199
3303
291
24.9
4099
4024
3349
3392
3716
400
26.1
4351
3972
4050
3767
4035
242
27.4
3752
3870
4131
3917
3918
158
28.6
3941
4064
3600
3773
3845
202
29.9
3747
3497
3405
3448
3524
153
31.1
3948
3675
3488
3364
3619
254
32.4
3818
3178
3407
3492
3474
265
33.6
3918
3425
3579
3323
3561
260
34.9
3886
3428
3396
3234
3486
280
36.1
3850
3073
3304
3074
3325
366
37.4
3591
3040
3332
3159
3280
239
38.6
3316
3011
2944
3124
3099
163
39.9
3267
2725
2955
3142
3022
236
41.1
3116
2830
2972
3019
2984
119
42.4
3148
3212
2800
2929
3022
191
43.6
3309
2822
3150
3182
3116
208
44.9
3392
2842
2934
2916
3021
250
46.1
3310
2932
3103
3085
3108
155
47.4
3301
2919
2962
3075
3064
171
48.6
3857
3307
3541
3244
3487
278
49.9
4409
3774
3676
3620
3870
365
51.1
3834
3995
3818
3874
3880
80
52.4
4037
3881
3610
3592
3780
217
53.6
3747
3731
3514
3608
3650
110
54.9
3907
3451
3525
3338
3555
247
56.1
3965
3167
3620
3404
3539
339
57.4
3733
3412
3604
3462
3553
145
58.6
3792
3344
3376
3424
3484
208
59.9
3870
3327
3465
3392
3513
245
61.1
3598
3153
3213
3205
3292
205
62.4
3697
3167
3007
3175
3262
301
63.6
3151
2771
3077
2885
2971
174
64.9
3203
2934
2705
3044
2972
209
66.1
2953
2527
2901
2845
2806
192
67.4
3241
2925
2873
2718
2939
220
68.6
3204
2665
2843
2776
2872
233
69.9
3161
2950
2659
3014
2946
211
71.1
3089
2860
2748
2780
2869
154
72.4
3570
2917
2989
3115
3148
293
73.6
3761
3686
3194
3269
3477
287
74.9
3836
3329
3493
3677
3584
220
76.1
3648
3583
3465
3833
3632
154
77.4
3736
3613
3363
3688
3600
166
Page A42 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3
nn p 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.200 4 4 0.886 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.200 4 4 0.886 4 4 1.000 4 4 0.114 4 4 0.686 4 4 0.686 4 4 0.057 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.057 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.334 4 4 0.886
group 1 vs 4
nn p 4 4 0.057 4 4 0.343 4 4 0.114 4 4 0.886 4 4 0.114 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.029 4 4 0.114 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.029 4 4 0.029 4 4 0.886 4 4 0.029 4 4 0.029 4 4 0.200 4 4 0.114 4 4 0.343 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.057 4 4 0.114 4 4 0.200 4 4 0.114 4 4 0.200 4 4 0.686 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.486 4 4 0.486 4 4 0.057 4 4 0.029 4 4 0.057 4 4 0.029 4 4 0.343 4 4 0.114 4 4 0.686 4 4 0.200 4 4 1.000 4 4 0.486 4 4 0.114 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.686 4 4 0.057 4 4 0.029 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.029 4 4 0.200 4 4 0.200
VO2, p-values Mann Whitney U-test
group 1 vs 6 group 3 vs 4 group 3 vs 6
nn p nn p nn p 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.057 4 4 0.200 4 4 0.029 4 4 0.114 4 4 0.343 4 4 0.114 4 4 0.200 4 4 0.486 4 4 0.057 4 4 0.114 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.114 4 4 0.200 4 4 0.029 4 4 0.686 4 4 0.029 4 4 0.029 4 4 0.114 4 4 0.057 4 4 0.029 4 4 0.200 4 4 0.200 4 4 0.114 4 4 0.486 4 4 0.486 4 4 0.057 4 4 0.486 4 4 0.200 4 4 0.486 4 4 0.114 4 4 0.200 4 4 0.057 4 4 0.486 4 4 0.343 4 4 0.029 4 4 0.114 4 4 0.114 4 4 0.029 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.057 4 4 0.029 4 4 0.486 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.057 4 4 0.029 4 4 0.029 4 4 0.886 4 4 0.114 4 4 0.029 4 4 0.200 4 4 0.114 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.029 4 4 0.029 4 4 0.686 4 4 0.200 4 4 0.114 4 4 0.486 4 4 0.200 4 4 0.114 4 4 0.486 4 4 0.486 4 4 0.029 4 4 0.343 4 4 0.200 4 4 0.057 4 4 0.486 4 4 0.200 4 4 0.029 4 4 0.686 4 4 0.114 4 4 0.057 4 4 1.000 4 4 0.686 4 4 0.029 4 4 0.686 4 4 0.200 4 4 0.057 4 4 0.686 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.200 4 4 0.057 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.114 4 4 0.200 4 4 0.886 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.029 4 4 0.486 4 4 0.200 4 4 0.114 4 4 0.486 4 4 0.200 4 4 0.029 4 4 0.686 4 4 0.029 4 4 0.029 4 4 0.686 4 4 0.200 4 4 0.029 4 4 0.486 4 4 0.200 4 4 0.029 4 4 0.886 4 4 0.029 4 4 0.029 4 4 0.140 4 4 0.029 4 4 0.114 4 4 0.486 4 4 0.114 4 4 0.200 4 4 0.886 4 4 0.486 4 4 0.057 4 4 0.343 4 4 0.114 4 4 0.057 4 4 0.886 4 4 0.343 4 4 0.057 4 4 0.343 4 4 0.114 4 4 0.029 4 4 0.057 4 4 0.057 4 4 0.114 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.057 4 4 0.029 4 4 0.200 4 4 0.029 4 4 0.486 4 4 0.029 4 4 0.057 4 4 0.343 4 4 0.057
group 4 vs 6
nn p 4 4 0.200 4 4 0.057 4 4 0.486 4 4 0.200 4 4 0.486 4 4 0.486 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.200 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.686 4 4 0.200 4 4 0.486 4 4 0.343 4 4 0.200 4 4 1.000 4 4 0.486 4 4 0.200 4 4 0.343 4 4 0.686 4 4 0.200 4 4 0.114 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.886 4 4 0.486 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.200 4 4 0.114 4 4 0.200 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.114 4 4 0.114 4 4 0.486 4 4 0.486
p<0.05
Page A43 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
CO2 production
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 1: HF control
VCO2 (ml/h/kg mouse)
Average VCO2 SD
cage 0101 cage 0105 cage 0201 cage 0205 (ml/h/kg mouse)
2929
3110
3620
2845
3126
348
2978
3408
3399
2787
3143
311
2781
3142
3042
2814
2945
176
2963
3025
3464
2655
3027
333
2773
2718
2664
2841
2749
76
2721
2759
3107
2565
2788
229
2478
2619
2736
2379
2553
157
2629
2308
2708
2391
2509
190
2418
2501
2567
2267
2438
130
2713
2207
2650
2315
2471
248
3008
2361
2357
2233
2490
351
2486
2419
2757
2474
2534
152
2362
2465
2357
2041
2306
184
2412
2231
2731
2440
2453
207
2563
2617
2602
2402
2546
99
3454
3237
3386
3369
3361
91
2775
2971
3388
3044
3044
256
3139
3105
3283
2578
3026
309
2804
2718
3414
3120
3014
318
2860
2620
3100
2592
2793
238
2861
2568
3243
2750
2856
285
2563
2568
3137
2730
2750
270
2599
2436
2976
2197
2552
327
2801
2505
2827
2280
2603
260
2500
2421
2436
2334
2423
68
2517
2267
2933
2241
2490
321
2627
2362
2813
2191
2498
276
2470
2518
2873
2345
2552
227
2748
2440
2656
2095
2485
290
2281
2470
2655
2354
2440
163
2448
2244
2505
2168
2341
161
2369
2400
2829
2209
2451
265
2594
2551
2874
2334
2588
222
2662
2631
2543
2510
2587
71
3258
3226
3635
3092
3303
233
3054
2903
3311
2877
3036
199
3010
3083
3407
2777
3069
261
2744
3012
3274
2839
2967
233
2610
2778
2975
2727
2773
152
2547
2717
3158
2521
2736
295
2403
2640
2997
2341
2595
297
2581
2784
3114
2383
2715
312
2571
2393
2915
2423
2576
239
2631
2574
2798
2088
2523
305
2574
2152
2597
2128
2363
258
2295
2478
2429
1822
2256
299
2211
2122
2445
1985
2191
193
2536
2019
2461
1914
2232
312
2132
1693
1936
2008
1943
185
2274
2161
2434
1765
2159
285
1943
2025
2320
2073
2090
162
1969
2239
2420
1970
2150
221
2228
1950
2546
2267
2248
244
2767
2843
3065
2691
2841
162
2844
3169
3474
2866
3088
297
2927
3054
3241
2818
3010
182
2559
2840
3150
2531
2770
289
2530
2822
2961
2683
2749
185
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
VCO2 (ml/h/kg mouse)
Average VCO2 SD
cage 0102 cage 0106 cage 0202 cage 0206 (ml/h/kg mouse)
3252
3003
2825
3076
3039
177
3223
3131
3321
3262
3234
80
2920
2824
3352
2920
3004
236
2958
2601
2985
2986
2883
188
3064
2849
3475
2676
3016
345
2699
2726
3161
2520
2776
272
2801
2151
2872
2743
2642
331
2572
2481
2697
2060
2452
276
2714
2400
3020
2190
2581
363
2585
2148
3083
2324
2535
407
2574
2524
2658
2156
2478
222
2164
2181
2852
2015
2303
374
2411
2426
3150
2209
2549
413
2300
2522
2630
2256
2427
179
2736
2188
3094
2372
2598
402
3201
3308
3794
3129
3358
300
3254
3232
3611
3130
3307
210
2985
2775
3335
3196
3073
245
3014
2175
3300
2743
2808
480
2775
2675
3305
2895
2913
277
2439
1920
3161
2671
2548
515
2504
2269
3092
2606
2618
346
2610
2010
2889
2378
2472
372
2617
2140
2941
2590
2572
329
2477
2465
2788
2208
2485
237
2899
1937
2859
2524
2555
445
2685
2169
3039
2355
2562
383
2683
2199
3043
2262
2547
394
2621
2408
2877
2293
2550
257
2474
1986
2836
2555
2463
354
2507
2197
2699
2100
2376
276
2548
2105
2784
2263
2425
301
2467
2244
2683
2211
2401
219
2764
2233
3009
2456
2615
341
3042
2866
3422
3123
3113
232
3429
3157
3477
3172
3309
168
3211
3074
3245
2974
3126
125
3047
2715
2949
2936
2912
140
3111
2508
3013
2564
2799
307
2896
2513
3032
2765
2802
221
2872
2595
2781
2578
2707
144
2476
2380
2820
2651
2582
194
2685
2602
2998
2616
2725
186
2488
2275
2807
2394
2491
228
2467
2477
2587
2067
2399
228
2183
2398
2543
2186
2328
176
1944
2326
2532
1776
2145
346
1948
2041
2791
2344
2281
380
2018
2013
2171
1721
1981
188
1832
1903
2347
2267
2087
257
1932
2169
2213
1916
2057
155
1855
1854
2316
1905
1982
224
2156
1965
2756
2343
2305
338
2564
2510
2797
2630
2625
125
3132
2821
3506
2895
3088
308
2833
2869
3164
2960
2956
149
2774
2421
3060
2920
2794
275
2464
2451
2963
2853
2683
264
Page A44 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 4: HF + 0.006 % PFHS
VCO2 (ml/h/kg mouse)
Average VCO2 SD
cage 0103 cage 0107 cage 0203 cage 0207 (ml/h/kg mouse)
3309
3390
3535
3348
3395
99
3243
3195
3526
3375
3335
148
2910
3285
3487
2961
3161
274
3013
2980
3360
2695
3012
273
2848
2670
3385
2981
2971
304
2803
3043
3222
3000
3017
172
2757
2340
2802
2483
2596
221
2834
2384
2957
2469
2661
278
2699
2685
2527
2316
2557
179
2641
2677
2628
2448
2598
102
2474
2658
2505
2519
2539
81
2501
3011
2615
2533
2665
236
2834
2835
2489
2379
2634
236
2638
2685
2634
2873
2708
113
2739
2772
2653
2586
2687
84
3504
3473
3331
3246
3389
121
3532
3535
3688
3585
3585
73
3236
3539
3498
3374
3412
137
3243
3256
3755
3475
3432
240
2940
3382
3493
3025
3210
269
3109
2912
3516
3001
3135
267
2575
2599
3708
2979
2965
528
2536
2804
3465
2755
2890
401
2419
1953
3136
2496
2501
487
2531
2461
2999
2664
2664
239
2613
2818
3063
2466
2740
259
2766
2612
2756
2243
2594
245
2533
2538
2510
2677
2564
76
2656
2834
2715
2186
2598
284
2367
2515
2371
2250
2376
109
2316
2481
2372
2195
2341
119
2273
2767
2861
2035
2484
395
2587
2927
2695
2742
2738
142
2771
2605
2987
2323
2672
280
3419
3205
3237
2691
3138
312
3884
3492
3775
3089
3560
355
3549
3283
4014
3281
3532
345
3537
3116
3646
3478
3444
230
3286
3411
3615
3060
3343
233
3013
2658
3123
3020
2954
204
2856
2761
2950
3005
2893
108
2482
2458
3004
2631
2644
252
2653
2516
2972
2505
2662
217
2446
2003
2852
2388
2422
348
2471
2132
2679
2317
2400
232
2444
2248
2508
2381
2395
111
2317
1959
2651
2239
2292
285
2384
2357
2281
2202
2306
82
2092
1863
2403
2467
2206
281
2089
1983
2360
2074
2127
163
2019
2148
2535
2135
2209
225
2254
2256
2476
2241
2307
113
2189
2394
2578
2122
2321
207
2792
2665
2648
2485
2648
126
3502
3130
3196
2820
3162
280
3708
2929
3755
3224
3404
397
3218
2380
3623
3083
3076
518
3192
2698
3327
2906
3031
283
Metabolic cages group 6: HF + 0.003 % PFOS
Time
VCO2 (ml/h/kg mouse)
Average VCO2
SD
(hr) cage 0104 cage 0108 cage 0204 cage 0208 (ml/h/kg mouse)
6.1
3618
3732
3629
3220
3550
226
7.4
3414
3149
3553
3346
3365
168
8.6
3077
3062
3321
2963
3106
152
9.9
3331
3294
3219
3015
3215
141
11.1
3459
3188
3324
2801
3193
284
12.4
3301
2650
2970
2656
2894
310
13.6
3344
2303
2875
2554
2769
449
14.9
3167
2783
2760
2326
2759
344
16.1
2754
2270
2407
2550
2495
207
17.4
2745
2591
2365
2139
2460
265
18.6
3329
2992
2511
2371
2801
441
19.9
3154
2288
2097
2433
2493
462
21.1
2765
2771
2441
2405
2595
200
22.4
2658
2817
2364
2466
2576
201
23.6
3248
2519
2387
2668
2705
380
24.9
3643
3328
2734
2974
3170
399
26.1
3711
3457
3256
3206
3408
230
27.4
3410
3409
3637
3476
3483
107
28.6
3586
3714
3225
3340
3466
224
29.9
3324
3143
3102
2929
3124
162
31.1
3436
3095
3183
2900
3153
223
32.4
3213
2431
3022
2895
2890
333
33.6
3040
2809
3004
2693
2887
165
34.9
3259
2784
2951
2688
2921
251
36.1
3172
2333
2737
2351
2648
396
37.4
3179
2503
2817
2633
2783
294
38.6
3080
2433
2362
2717
2648
326
39.9
2992
2068
2449
2838
2587
414
41.1
2701
2306
2399
2627
2508
186
42.4
2828
2532
2224
2310
2474
270
43.6
2695
2234
2681
2499
2527
215
44.9
2775
2233
2426
2409
2461
227
46.1
2741
2411
2500
2573
2556
140
47.4
2766
2411
2616
2534
2582
149
48.6
3454
2739
3044
2867
3026
312
49.9
3931
3266
3167
3104
3367
382
51.1
3393
3634
3407
3247
3420
160
52.4
3656
3495
3236
3014
3350
283
53.6
3109
3309
3021
3121
3140
121
54.9
3360
2940
2868
2811
2995
249
56.1
3313
2510
2896
2675
2849
348
57.4
3183
2708
2683
2661
2809
250
58.6
3210
2732
2631
2576
2787
289
59.9
3309
2775
2552
2529
2791
363
61.1
3020
2472
2453
2522
2617
270
62.4
2882
2549
2424
2522
2594
199
63.6
2636
2062
2504
2233
2359
260
64.9
2675
2302
2208
2333
2379
204
66.1
2419
2040
2197
2131
2197
161
67.4
2724
2379
2060
2004
2291
332
68.6
2533
2023
2195
1971
2180
254
69.9
2450
2344
2016
2234
2261
185
71.1
2505
2242
1969
2016
2183
246
72.4
3034
2294
2265
2426
2505
360
73.6
3260
3089
2531
2702
2895
337
74.9
3398
2900
2924
3037
3065
230
76.1
3198
3204
3044
3244
3172
88
77.4
3051
3148
2734
3064
2999
182
Page A45 of A172
Appendix to TNO-report of 3M-02
Final version
Time
(hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3
nn p 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.486 4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.486 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.114 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.114 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.886
group 1 vs 4
nn p 4 4 0.343 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.200 4 4 0.200 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.889 4 4 0.343 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.114 4 4 0.686 4 4 0.029 4 4 0.057 4 4 0.114 4 4 0.114 4 4 0.200 4 4 0.489 4 4 0.343 4 4 0.686 4 4 0.057 4 4 0.343 4 4 0.886 4 4 0.486 4 4 0.486 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.686 4 4 0.057 4 4 0.114 4 4 0.057 4 4 0.029 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.686 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.200 4 4 0.886 4 4 0.114 4 4 0.886 4 4 0.200 4 4 0.486 4 4 0.200
VCO2, p-values Mann Whitney U-test
group 1 vs 6 group 3 vs 4 group 3 vs 6
nn p nn p nn p 4 4 0.114 4 4 0.029 4 4 0.057 4 4 0.343 4 4 0.486 4 4 0.200 4 4 0.343 4 4 0.486 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.029 4 4 0.057 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.200 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.200 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.200 4 4 0.486 4 4 0.114 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.029 4 4 0.343 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.686 4 4 0.114 4 4 0.200 4 4 0.486 4 4 0.029 4 4 0.057 4 4 0.029 4 4 0.114 4 4 0.114 4 4 0.057 4 4 0.057 4 4 0.114 4 4 0.200 4 4 0.200 4 4 0.200 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.114 4 4 0.343 4 4 0.114 4 4 0.343 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.486 4 4 1.000 4 4 1.000 4 4 1.000 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.686 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.057 4 4 0.343 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.200 4 4 0.886 4 4 0.886 4 4 0.200 4 4 0.343 4 4 0.886 4 4 0.114 4 4 0.029 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.057 4 4 0.029 4 4 0.057 4 4 0.114 4 4 0.200 4 4 0.486 4 4 0.486 4 4 0.343 4 4 0.200 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.200 4 4 0.200 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.200 4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.114 4 4 0.686 4 4 0.114 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.114 4 4 0.343 4 4 0.114 4 4 0.886 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.200 4 4 0.114 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.200 4 4 0.886 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.486 4 4 1.000 4 4 0.114 4 4 0.486 4 4 0.057 4 4 0.343 4 4 0.057 4 4 0.114 4 4 0.200 4 4 0.114
group 4 vs 6
nn p 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.486 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.686 4 4 1.000 4 4 0.686 4 4 1.000 4 4 1.000 4 4 0.686 4 4 0.200 4 4 0.886 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.200 4 4 1.000 4 4 0.114 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.343 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.486 4 4 0.200 4 4 0.343 4 4 0.114 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.343 4 4 0.200 4 4 1.000 4 4 1.000
04-12-2006
p<0.05
Page A46 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Respiratory exchange ratio
Metabolic cages group 1: HF control
Respiratory exchange ratio
cage 0101 cage 0105 cage 0201 cage 0205
0.960
0.861
1.015
0.895
1.025
0.902
1.037
0.934
0.997
0.900
0.994
0.931
0.971
0.854
0.962
0.909
1.014
0.848
0.906
0.884
0.966
0.933
0.975
0.885
0.939
0.841
1.000
0.892
0.981
0.839
0.976
0.883
1.008
0.890
0.926
0.845
0.962
0.817
0.917
0.837
0.969
0.824
0.905
0.815
0.896
0.827
0.936
0.792
0.906
0.821
0.895
0.803
0.877
0.824
0.966
0.843
0.852
0.876
0.867
0.862
0.908
0.878
0.890
0.857
0.941
0.886
0.960
0.785
0.923
0.877
0.936
0.853
0.965
0.823
1.007
0.877
0.963
0.802
0.995
0.866
0.961
0.784
0.977
0.903
0.926
0.805
0.952
0.856
0.948
0.765
0.876
0.747
0.889
0.809
0.881
0.797
0.879
0.780
0.907
0.870
0.882
0.764
0.894
0.821
0.985
0.920
0.998
0.865
1.030
0.936
1.040
0.881
1.027
0.916
1.012
0.856
0.972
0.882
0.947
0.827
0.962
0.890
0.946
0.814
0.930
0.892
0.985
0.863
0.940
0.882
0.959
0.845
0.905
0.920
0.941
0.908
0.913
0.916
0.968
0.915
0.881
0.886
0.881
0.856
0.907
0.888
0.964
0.901
0.919
0.870
0.981
0.910
0.892
0.870
0.923
0.871
0.832
0.827
0.975
0.812
0.907
0.816
0.943
0.828
0.916
0.865
0.956
0.813
0.910
0.831
0.888
0.781
0.903
0.817
0.899
0.787
0.964
0.763
0.931
0.802
0.963
0.861
0.875
0.766
0.896
0.774
0.830
0.772
0.933
0.784
0.817
0.759
0.890
0.745
0.810
0.783
0.876
0.794
0.857
0.721
0.811
0.758
0.907
0.772
0.777
0.839
0.926
0.785
0.852
0.775
0.903
0.830
0.887
0.901
0.912
0.814
0.855
0.909
0.890
0.831
0.891
0.921
0.931
0.854
0.870
0.873
0.930
0.866
0.888
0.844
0.872
0.864
Average RER 0.933 0.974 0.955 0.924 0.913 0.940 0.918 0.920 0.917 0.883 0.878 0.863 0.856 0.878 0.864 0.883 0.893 0.897 0.918 0.906 0.906 0.885 0.834 0.844 0.859 0.840 0.942 0.972 0.953 0.907 0.903 0.917 0.906 0.918 0.928 0.876 0.915 0.920 0.889 0.861 0.874 0.887 0.853 0.851 0.865 0.867 0.818 0.823 0.807 0.812 0.812 0.832 0.840 0.878 0.871 0.899 0.885 0.867
SD
0.069 0.067 0.048 0.054 0.072 0.041 0.068 0.070 0.069 0.068 0.073 0.065 0.052 0.063 0.010 0.022 0.079 0.039 0.083 0.089 0.087 0.067 0.095 0.047 0.055 0.060 0.062 0.076 0.081 0.066 0.067 0.053 0.052 0.016 0.027 0.013 0.033 0.046 0.025 0.076 0.062 0.062 0.058 0.059 0.097 0.080 0.059 0.077 0.062 0.070 0.067 0.069 0.053 0.044 0.035 0.035 0.030 0.018
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 3: HF + 0.03 % PFBS
Respiratory exchange ratio
Average
cage 0102 cage 0106 cage 0202 cage 0206
RER
0.937
0.894
0.893
0.878
0.900
0.950
0.919
0.987
0.903
0.940
0.959
0.882
0.972
0.887
0.925
0.931
0.884
0.959
0.856
0.908
0.944
0.910
0.909
0.842
0.901
0.922
0.889
0.932
0.869
0.903
0.920
0.800
0.928
0.895
0.886
0.923
0.867
0.867
0.805
0.866
0.946
0.831
0.908
0.851
0.884
0.927
0.808
0.874
0.841
0.862
0.836
0.832
0.863
0.807
0.834
0.831
0.804
0.916
0.803
0.839
0.855
0.863
0.921
0.763
0.851
0.833
0.858
0.847
0.780
0.830
0.858
0.846
0.874
0.849
0.857
0.834
0.878
0.913
0.871
0.874
0.894
0.812
0.895
0.818
0.855
0.901
0.856
0.943
0.875
0.894
0.887
0.809
0.982
0.893
0.892
0.858
0.771
0.975
0.855
0.865
0.851
0.743
0.896
0.855
0.837
0.877
0.783
0.862
0.859
0.845
0.871
0.744
0.867
0.823
0.826
0.850
0.779
0.842
0.830
0.825
0.837
0.802
0.835
0.801
0.819
0.922
0.765
0.852
0.871
0.852
0.994
0.868
0.960
0.929
0.938
0.988
0.870
0.957
0.917
0.933
0.998
0.920
0.955
0.904
0.944
0.949
0.901
0.864
0.811
0.881
0.916
0.931
0.829
0.766
0.860
0.912
0.842
0.825
0.824
0.851
0.912
0.851
0.833
0.821
0.854
0.906
0.883
0.900
0.893
0.895
0.854
0.919
0.888
0.861
0.881
0.890
0.902
0.902
0.809
0.876
0.929
0.892
0.882
0.845
0.887
0.958
0.907
0.892
0.893
0.912
0.928
0.895
0.873
0.808
0.876
0.938
0.875
0.853
0.840
0.877
0.930
0.855
0.814
0.809
0.852
0.864
0.826
0.829
0.812
0.833
0.870
0.829
0.832
0.790
0.830
0.815
0.800
0.811
0.783
0.802
0.922
0.786
0.840
0.784
0.833
0.884
0.858
0.880
0.803
0.856
0.809
0.860
0.861
0.746
0.819
0.833
0.854
0.863
0.801
0.838
0.793
0.877
0.770
0.719
0.790
0.759
0.822
0.778
0.790
0.787
0.783
0.814
0.789
0.760
0.787
0.754
0.758
0.811
0.767
0.772
0.794
0.844
0.841
0.835
0.828
0.787
0.873
0.794
0.830
0.821
0.894
0.867
0.862
0.809
0.858
0.879
0.856
0.875
0.854
0.866
0.892
0.826
0.893
0.850
0.866
0.821
0.826
0.826
0.853
0.831
SD
0.025 0.037 0.047 0.046 0.042 0.029 0.059 0.048 0.053 0.051 0.023 0.053 0.065 0.034 0.013 0.032 0.046 0.037 0.071 0.084 0.065 0.042 0.059 0.032 0.020 0.065 0.053 0.051 0.042 0.059 0.077 0.041 0.040 0.010 0.030 0.045 0.034 0.031 0.051 0.044 0.056 0.022 0.033 0.014 0.065 0.037 0.054 0.027 0.066 0.027 0.022 0.026 0.023 0.039 0.036 0.013 0.033 0.014
Page A47 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 4: HF + 0.006 % PFHS
Respiratory exchange ratio
Average
cage 0103 cage 0107 cage 0203 cage 0207
RER
0.927
0.918
0.932
0.913
0.922
0.944
0.925
0.942
0.944
0.939
0.889
0.920
0.938
0.922
0.917
0.913
0.905
0.907
0.894
0.905
0.885
0.881
0.896
0.920
0.895
0.858
0.889
0.876
0.895
0.880
0.848
0.843
0.869
0.852
0.853
0.901
0.817
0.861
0.860
0.860
0.862
0.791
0.795
0.802
0.812
0.851
0.807
0.823
0.884
0.841
0.820
0.918
0.843
0.837
0.854
0.810
0.952
0.803
0.884
0.862
0.919
0.943
0.842
0.839
0.886
0.863
0.905
0.824
0.857
0.862
0.845
0.880
0.802
0.845
0.843
0.868
0.787
0.879
0.888
0.855
0.829
0.828
0.875
0.871
0.851
0.855
0.912
0.912
0.899
0.894
0.922
0.968
0.948
0.926
0.941
0.933
0.959
0.960
0.934
0.947
0.915
0.919
0.963
0.932
0.932
0.815
0.912
0.930
0.916
0.893
0.795
0.872
0.882
0.833
0.846
0.813
0.806
0.870
0.812
0.825
0.794
0.851
0.896
0.801
0.836
0.866
0.938
0.866
0.749
0.855
0.942
0.939
0.856
0.845
0.895
0.890
0.945
0.812
0.929
0.894
0.903
0.968
0.843
0.867
0.895
0.812
0.919
0.795
0.821
0.837
0.781
0.925
0.796
0.794
0.824
0.813
0.923
0.829
0.754
0.830
0.840
0.892
0.884
0.857
0.868
0.860
0.910
0.915
0.861
0.887
0.925
0.913
0.924
0.871
0.908
0.922
0.882
0.921
0.839
0.891
0.939
0.890
0.922
0.885
0.909
0.964
0.919
0.931
0.926
0.935
0.945
0.958
0.940
0.922
0.941
0.900
0.909
0.840
0.908
0.889
0.863
0.898
0.830
0.893
0.871
0.819
0.819
0.803
0.862
0.826
0.814
0.801
0.803
0.792
0.803
0.823
0.777
0.829
0.810
0.810
0.820
0.803
0.828
0.839
0.822
0.809
0.786
0.834
0.849
0.819
0.801
0.777
0.795
0.838
0.803
0.817
0.811
0.745
0.832
0.801
0.802
0.781
0.753
0.806
0.786
0.754
0.798
0.785
0.771
0.777
0.754
0.786
0.820
0.796
0.789
0.789
0.769
0.819
0.803
0.795
0.763
0.802
0.846
0.809
0.805
0.862
0.809
0.804
0.850
0.831
0.924
0.781
0.841
0.822
0.842
0.939
0.789
0.877
0.846
0.863
0.955
0.799
0.878
0.888
0.880
0.936
0.836
0.841
0.860
0.868
SD
0.008 0.009 0.020 0.008 0.018 0.016 0.012 0.034 0.034 0.034 0.044 0.070 0.053 0.033 0.032 0.046 0.026 0.027 0.021 0.015 0.022 0.053 0.040 0.030 0.048 0.078 0.052 0.059 0.055 0.056 0.068 0.070 0.024 0.030 0.025 0.040 0.026 0.020 0.015 0.033 0.031 0.025 0.009 0.023 0.015 0.028 0.026 0.039 0.025 0.019 0.027 0.021 0.034 0.029 0.060 0.063 0.064 0.047
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
Metabolic cages group 6: HF + 0.003 % PFOS
Respiratory exchange ratio
Average
cage 0104 cage 0108 cage 0204 cage 0208
RER
0.920
0.863
0.914
0.888
0.896
0.903
0.790
0.935
0.916
0.886
0.923
0.802
0.903
0.878
0.876
0.900
0.871
0.899
0.877
0.887
0.894
0.874
0.911
0.891
0.892
0.909
0.821
0.887
0.850
0.867
0.888
0.741
0.893
0.799
0.830
0.882
0.796
0.848
0.774
0.825
0.826
0.782
0.777
0.839
0.806
0.855
0.817
0.787
0.771
0.808
0.891
0.850
0.782
0.814
0.834
0.853
0.772
0.718
0.805
0.787
0.835
0.895
0.828
0.785
0.836
0.806
0.845
0.793
0.818
0.815
0.868
0.784
0.771
0.830
0.813
0.892
0.828
0.814
0.875
0.852
0.851
0.871
0.804
0.848
0.844
0.908
0.881
0.879
0.886
0.888
0.911
0.914
0.894
0.885
0.901
0.886
0.900
0.909
0.848
0.885
0.868
0.840
0.912
0.862
0.871
0.843
0.764
0.888
0.827
0.830
0.776
0.819
0.836
0.812
0.811
0.836
0.812
0.865
0.830
0.836
0.824
0.756
0.828
0.761
0.792
0.890
0.825
0.847
0.839
0.850
0.929
0.807
0.801
0.869
0.852
0.916
0.759
0.828
0.902
0.851
0.867
0.813
0.807
0.871
0.839
0.898
0.784
0.793
0.790
0.816
0.808
0.787
0.846
0.782
0.806
0.816
0.785
0.830
0.825
0.814
0.827
0.820
0.804
0.834
0.821
0.836
0.826
0.885
0.822
0.842
0.896
0.827
0.859
0.883
0.866
0.890
0.864
0.860
0.857
0.868
0.885
0.909
0.891
0.836
0.880
0.905
0.901
0.897
0.837
0.885
0.829
0.885
0.860
0.861
0.859
0.860
0.852
0.813
0.841
0.841
0.835
0.792
0.799
0.784
0.802
0.852
0.795
0.743
0.768
0.790
0.846
0.817
0.779
0.750
0.798
0.855
0.834
0.735
0.745
0.792
0.841
0.783
0.762
0.784
0.793
0.782
0.803
0.806
0.795
0.797
0.838
0.741
0.813
0.772
0.791
0.833
0.776
0.816
0.763
0.797
0.820
0.807
0.753
0.749
0.782
0.833
0.811
0.715
0.736
0.774
0.787
0.758
0.766
0.709
0.755
0.773
0.792
0.757
0.733
0.764
0.809
0.782
0.715
0.722
0.757
0.850
0.786
0.755
0.775
0.792
0.868
0.838
0.788
0.825
0.830
0.885
0.871
0.835
0.825
0.854
0.876
0.893
0.878
0.846
0.873
0.814
0.869
0.811
0.832
0.832
SD
0.026 0.066 0.053 0.015 0.015 0.039 0.074 0.049 0.031 0.037 0.047 0.057 0.045 0.022 0.044 0.037 0.028 0.013 0.014 0.027 0.030 0.051 0.025 0.022 0.039 0.028 0.060 0.073 0.034 0.055 0.029 0.020 0.013 0.029 0.030 0.015 0.031 0.032 0.023 0.021 0.023 0.047 0.042 0.061 0.034 0.011 0.043 0.033 0.037 0.057 0.033 0.025 0.046 0.041 0.033 0.028 0.020 0.027
Page A48 of A172
Appendix to TNO-report of 3M-02
Final version
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p 4 4 0.486 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.200 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.343 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.114 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.343 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.686 4 4 1.000 4 4 1.000 4 4 0.486 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.114 4 4 0.886 4 4 0.114 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.057
Respiratory exchange ratio, p-values Mann Whitney U-test group 1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6 nn p nn p nn p nn p nn p 4 4 1.000 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.200 4 4 0.886 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.057 4 4 0.343 4 4 0.114 4 4 1.000 4 4 0.343 4 4 0.343 4 4 0.486 4 4 0.343 4 4 1.000 4 4 0.686 4 4 0.114 4 4 1.000 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.114 4 4 0.114 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.114 4 4 1.000 4 4 0.343 4 4 0.114 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.057 4 4 0.029 4 4 0.114 4 4 0.057 4 4 0.686 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.486 4 4 1.000 4 4 0.686 4 4 1.000 4 4 0.200 4 4 0.886 4 4 0.486 4 4 0.200 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.114 4 4 0.343 4 4 0.486 4 4 0.057 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.486 4 4 0.343 4 4 0.886 4 4 0.486 4 4 1.000 4 4 0.343 4 4 0.343 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.886 4 4 1.000 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.886 4 4 1.000 4 4 0.343 4 4 0.686 4 4 0.029 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.029 4 4 0.886 4 4 0.486 4 4 0.029 4 4 0.486 4 4 0.029 4 4 1.000 4 4 0.343 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.486 4 4 0.343 4 4 0.886 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.686 4 4 0.114 4 4 0.886 4 4 0.343 4 4 0.343 4 4 1.000 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.343 4 4 0.114 4 4 0.486 4 4 0.114 4 4 0.486 4 4 0.343 4 4 0.114 4 4 0.200 4 4 0.029 4 4 0.343 4 4 0.114 4 4 0.114 4 4 0.486 4 4 0.114 4 4 0.200 4 4 0.114 4 4 0.057 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.114 4 4 0.029 4 4 0.686 4 4 0.343 4 4 1.000 4 4 0.486 4 4 0.029 4 4 0.486 4 4 0.200 4 4 0.029 4 4 0.343 4 4 0.029 4 4 1.000 4 4 0.057 4 4 0.114 4 4 0.686 4 4 0.029 4 4 0.200 4 4 0.686 4 4 0.114 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.200 4 4 0.200 4 4 0.686 4 4 0.029 4 4 0.057 4 4 0.114 4 4 0.057 4 4 0.486 4 4 0.029 4 4 0.343 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.200 4 4 1.000 4 4 0.114 4 4 0.486 4 4 0.114 4 4 0.057 4 4 0.200 4 4 0.057 4 4 0.486 4 4 0.200 4 4 0.200 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.343 4 4 1.000 4 4 0.486 4 4 0.343 4 4 0.686 4 4 1.000 4 4 1.000 4 4 1.000 4 4 0.343 4 4 1.000 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.343 4 4 0.200 4 4 0.114 4 4 0.200 4 4 0.886 4 4 0.686 4 4 0.486 4 4 0.486 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.200 4 4 0.114 4 4 1.000 4 4 0.686 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.486 4 4 0.486 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.200 4 4 0.886 4 4 0.200 4 4 0.343 4 4 0.686 4 4 0.114 4 4 0.200 4 4 0.886 4 4 0.200 4 4 0.343 4 4 0.114 4 4 0.686 4 4 0.057 4 4 0.200 4 4 0.114 4 4 0.057 4 4 0.686 4 4 0.200 4 4 0.200 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.486 4 4 1.000 4 4 0.486 4 4 0.114 4 4 0.886 4 4 0.686 4 4 0.886 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.114 4 4 0.114 4 4 0.886 4 4 0.200
04-12-2006
p<0.05
Page A49 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
D6. Energy expenditure
Metabolic cages group 1: HF control
Time
Energy expenditure (kcal/h/kg mouse)
Average energy expenditure SD
(hr) cage 0101 cage 0105 cage 0201 cage 0205
(kcal/h/kg mouse)
6.1
15.26
17.60
18.09
15.65
16.65
1.40
7.4
14.77
18.58
16.69
14.83
16.22
1.81
8.6
14.06
17.22
15.46
15.02
15.44
1.32
9.9
15.29
17.23
18.03
14.38
16.23
1.69
11.1
13.87
15.56
14.51
15.73
14.92
0.88
12.4
14.09
14.71
16.01
14.24
14.76
0.88
13.6
13.11
15.06
13.84
13.08
13.77
0.93
14.9
13.48
13.36
13.89
13.27
13.50
0.27
16.1
12.13
13.82
13.73
13.00
13.17
0.78
17.4
14.07
13.01
14.23
13.37
13.67
0.58
18.6
15.59
13.78
12.86
13.16
13.85
1.22
19.9
13.62
14.11
14.61
14.87
14.30
0.56
21.1
12.84
14.44
12.93
12.21
13.11
0.95
22.4
13.44
13.08
14.15
14.04
13.68
0.51
23.6
14.61
14.60
14.57
13.58
14.34
0.50
24.9
18.76
18.08
18.68
19.18
18.67
0.45
26.1
14.71
16.46
17.67
18.47
16.83
1.64
27.4
16.80
17.25
17.38
14.72
16.54
1.24
28.6
14.60
15.94
17.12
17.36
16.25
1.27
29.9
14.85
15.69
15.70
14.61
15.21
0.57
31.1
14.90
15.61
16.65
14.99
15.54
0.80
32.4
13.70
15.30
16.48
15.57
15.26
1.16
33.6
13.66
15.14
16.64
13.85
14.82
1.38
34.9
15.51
14.87
15.70
13.65
14.93
0.93
36.1
13.93
14.80
13.31
13.10
13.78
0.76
37.4
13.90
14.00
16.10
13.12
14.28
1.28
38.6
13.43
12.69
14.25
12.34
13.18
0.84
39.9
12.20
13.39
14.13
13.03
13.19
0.80
41.1
13.65
13.20
13.31
11.93
13.02
0.75
42.4
11.75
13.71
13.91
13.69
13.27
1.01
43.6
12.71
12.39
13.16
12.74
12.75
0.32
44.9
12.64
13.18
14.44
12.48
13.19
0.89
46.1
13.72
14.15
14.99
13.38
14.06
0.70
47.4
14.48
14.15
13.45
13.61
13.93
0.48
48.6
17.69
17.46
18.78
16.77
17.67
0.84
49.9
16.95
16.08
18.41
16.32
16.94
1.05
51.1
16.37
17.06
17.66
15.18
16.57
1.06
52.4
14.77
16.94
16.72
15.37
15.95
1.05
53.6
14.41
15.64
15.92
15.35
15.33
0.66
54.9
14.84
15.90
16.22
14.90
15.46
0.70
56.1
13.10
15.64
15.87
13.62
14.56
1.40
57.4
13.93
15.75
16.27
14.08
15.01
1.18
58.6
13.93
13.92
16.08
14.73
14.67
1.02
59.9
14.30
15.21
15.35
12.69
14.39
1.22
61.1
13.41
13.41
13.86
12.72
13.35
0.47
62.4
11.94
14.05
13.54
11.28
12.70
1.31
63.6
12.07
12.94
14.22
12.19
12.86
0.99
64.9
13.53
12.28
14.41
11.88
13.02
1.16
66.1
11.78
10.77
11.51
12.25
11.58
0.62
67.4
12.67
12.95
13.74
11.51
12.72
0.92
68.6
11.52
12.61
12.64
12.73
12.37
0.57
69.9
12.09
12.89
12.94
11.99
12.48
0.51
71.1
12.71
11.98
13.88
13.18
12.94
0.80
72.4
15.29
15.54
16.61
15.93
15.84
0.57
73.6
16.15
17.21
19.20
16.67
17.31
1.33
74.9
16.12
16.43
17.30
16.02
16.47
0.58
76.1
14.28
15.90
16.75
14.24
15.29
1.24
77.4
14.01
16.21
16.58
15.16
15.49
1.16
Metabolic cages group 3: HF + 0.03 % PFBS
Time
Energy expenditure (kcal/h/kg mouse)
Average energy expenditure SD
(hr) cage 0102 cage 0106 cage 0202 cage 0206
(kcal/h/kg mouse)
6.1
17.43
16.55
15.54
17.20
16.68
0.85
7.4
17.09
16.86
16.94
17.80
17.17
0.43
8.6
15.39
15.70
17.29
16.16
16.13
0.83
9.9
15.91
14.45
15.57
16.99
15.73
1.05
11.1
16.34
15.43
18.84
15.42
16.51
1.61
12.4
14.64
15.03
16.85
14.16
15.17
1.17
13.6
15.19
12.93
15.35
15.09
14.64
1.14
14.9
13.95
13.93
15.14
12.27
13.82
1.18
16.1
14.44
13.92
16.41
12.52
14.32
1.61
17.4
14.01
12.76
17.22
13.38
14.34
1.99
18.6
14.98
14.58
15.00
12.81
14.34
1.04
19.9
12.72
13.02
15.40
12.07
13.30
1.45
21.1
13.81
13.65
16.90
13.71
14.52
1.59
22.4
13.46
14.25
15.08
13.72
14.13
0.72
23.6
15.62
12.57
17.30
13.56
14.76
2.12
24.9
18.75
18.37
20.52
17.56
18.80
1.25
26.1
18.06
19.16
19.87
18.43
18.88
0.80
27.4
16.48
15.80
17.65
17.88
16.95
0.98
28.6
16.92
12.95
16.88
15.09
15.46
1.88
29.9
15.90
16.54
17.00
16.45
16.47
0.45
31.1
14.09
12.21
17.31
15.23
14.71
2.13
32.4
14.12
13.77
17.36
14.77
15.00
1.62
33.6
14.78
12.74
16.24
13.97
14.43
1.47
34.9
15.12
13.05
16.89
15.06
15.03
1.57
36.1
14.52
14.64
16.12
13.22
14.62
1.19
37.4
15.83
12.02
16.36
14.18
14.60
1.95
38.6
13.76
12.26
15.82
12.58
13.61
1.61
39.9
13.81
12.37
15.88
12.21
13.57
1.70
41.1
13.39
12.95
15.06
12.52
13.48
1.11
42.4
13.12
10.84
16.01
15.10
13.77
2.29
43.6
13.64
11.69
15.73
13.03
13.52
1.68
44.9
13.95
12.08
16.21
13.22
13.86
1.74
46.1
13.47
12.78
15.51
12.95
13.68
1.25
47.4
15.18
12.38
16.46
13.52
14.39
1.79
48.6
17.57
15.45
18.91
17.72
17.41
1.44
49.9
19.09
17.24
18.98
18.82
18.53
0.87
51.1
17.30
16.92
17.99
17.07
17.32
0.48
52.4
16.05
14.75
16.27
16.18
15.81
0.71
53.6
16.77
13.77
16.86
15.23
15.66
1.46
54.9
15.50
14.08
17.30
15.96
15.71
1.33
56.1
15.48
14.73
16.46
15.33
15.50
0.72
57.4
14.11
13.92
16.45
15.71
15.05
1.23
58.6
15.24
15.13
17.39
15.86
15.91
1.04
59.9
14.87
13.68
16.69
14.54
14.94
1.27
61.1
13.37
15.05
14.93
12.60
13.99
1.20
62.4
12.14
13.59
14.17
13.05
13.24
0.86
63.6
11.67
13.16
14.32
11.27
12.60
1.40
64.9
11.45
11.64
15.69
13.95
13.18
2.02
66.1
12.32
11.23
13.41
11.25
12.05
1.04
67.4
11.54
11.21
14.36
13.65
12.69
1.55
68.6
11.90
12.82
13.42
11.92
12.52
0.74
69.9
11.77
11.55
13.71
11.82
12.21
1.00
71.1
13.05
11.30
15.86
13.56
13.44
1.88
72.4
15.71
14.02
16.76
15.30
15.44
1.13
73.6
17.40
15.88
19.87
17.20
17.59
1.67
74.9
15.92
16.23
17.67
16.87
16.68
0.77
76.1
15.41
14.16
16.82
16.69
15.77
1.25
77.4
14.65
14.28
17.27
16.29
15.62
1.40
Page A50 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Metabolic cages group 4: HF + 0.006 % PFHS
Time
Energy expenditure (kcal/h/kg mouse)
Average energy expenditure SD
(hr) cage 0103 cage 0107 cage 0203 cage 0207
(kcal/h/kg mouse)
6.1
17.72
18.32
18.90
18.10
18.26
0.49
7.4
17.11
17.10
18.69
17.78
17.67
0.75
8.6
16.08
17.64
18.56
15.91
17.05
1.27
9.9
16.29
16.21
18.34
14.84
16.42
1.44
11.1
15.79
14.83
18.65
16.03
16.33
1.64
12.4
15.90
16.76
18.05
16.46
16.79
0.91
13.6
15.81
13.50
15.81
14.17
14.82
1.17
14.9
15.50
14.04
16.75
14.00
15.07
1.32
16.1
15.27
16.11
15.26
13.82
15.12
0.95
17.4
15.08
15.83
15.47
13.59
14.99
0.99
18.6
14.56
14.33
14.50
14.54
14.48
0.11
19.9
14.82
15.78
15.58
14.07
15.06
0.78
21.1
15.26
14.93
14.36
13.74
14.57
0.67
22.4
14.90
14.62
15.33
16.29
15.28
0.73
23.6
15.70
15.43
15.87
14.86
15.46
0.44
24.9
19.72
21.15
18.58
17.89
19.33
1.43
26.1
20.61
20.63
20.66
20.08
20.50
0.28
27.4
18.46
19.15
18.98
18.45
18.76
0.36
28.6
17.40
16.86
19.80
18.59
18.16
1.31
29.9
15.63
17.63
18.26
16.07
16.90
1.25
31.1
16.78
15.69
18.33
15.98
16.69
1.18
32.4
15.19
14.09
19.84
16.07
16.29
2.50
33.6
15.21
15.71
19.30
15.97
16.55
1.86
34.9
14.31
11.67
17.68
14.78
14.61
2.46
36.1
15.18
14.05
16.54
15.91
15.42
1.07
37.4
14.73
15.03
17.36
15.56
15.67
1.18
38.6
14.63
13.86
15.71
12.89
14.27
1.20
39.9
13.99
13.36
14.92
14.30
14.14
0.65
41.1
14.49
14.67
15.65
12.34
14.29
1.39
42.4
14.01
13.51
14.35
13.17
13.76
0.52
43.6
14.08
13.28
14.29
13.21
13.71
0.55
44.9
13.47
14.84
16.71
12.78
14.45
1.73
46.1
14.88
16.08
15.00
15.51
15.37
0.55
47.4
15.64
14.14
16.17
13.12
14.77
1.40
48.6
18.28
17.34
17.39
15.11
17.03
1.35
49.9
20.86
19.42
20.34
17.84
19.62
1.33
51.1
18.77
18.11
21.60
18.16
19.16
1.65
52.4
18.37
16.76
19.49
18.59
18.30
1.14
53.6
17.30
17.80
19.21
16.43
17.68
1.16
54.9
16.46
14.44
18.06
16.42
16.35
1.48
56.1
16.08
15.13
17.28
16.52
16.25
0.90
57.4
14.64
14.47
18.02
14.88
15.50
1.69
58.6
15.67
15.08
17.87
15.07
15.92
1.33
59.9
14.27
12.31
16.74
14.16
14.37
1.82
61.1
14.48
12.77
15.72
13.40
14.09
1.30
62.4
14.56
13.63
14.62
13.60
14.11
0.56
63.6
13.88
12.02
15.96
12.96
13.70
1.69
64.9
13.99
13.95
14.50
12.77
13.80
0.73
66.1
12.49
11.40
15.11
14.61
13.40
1.75
67.4
13.11
11.93
14.36
12.75
13.04
1.01
68.6
12.66
12.96
14.99
12.77
13.35
1.10
69.9
13.64
13.88
14.56
13.32
13.85
0.53
71.1
13.62
14.26
14.77
12.59
13.81
0.94
72.4
15.79
15.84
15.90
14.20
15.43
0.82
73.6
18.77
19.12
18.49
16.54
18.23
1.16
74.9
19.64
17.74
21.04
18.51
19.23
1.43
76.1
16.81
14.27
20.24
17.03
17.09
2.45
77.4
16.94
15.58
19.19
16.40
17.03
1.55
Metabolic cages group 6: HF + 0.003 % PFOS
Time
Energy expenditure (kcal/h/kg mouse)
Average energy expenditure
SD
(hr) cage 0104 cage 0108 cage 0204 cage 0208
(kcal/h/kg mouse)
6.1
19.47
21.08
19.61
17.81
19.49
1.34
7.4
18.62
19.06
18.86
18.04
18.65
0.44
8.6
16.51
18.30
18.11
16.54
17.36
0.97
9.9
18.20
18.49
17.62
16.84
17.79
0.73
11.1
19.01
17.84
18.00
15.43
17.57
1.52
12.4
17.93
15.57
16.43
15.19
16.28
1.22
13.6
18.49
14.69
15.83
15.23
16.06
1.68
14.9
17.61
16.67
15.79
14.34
16.10
1.39
16.1
16.12
13.87
14.75
14.73
14.87
0.93
17.4
15.64
15.29
14.35
13.23
14.63
1.08
18.6
18.32
17.05
15.27
13.96
16.15
1.92
19.9
17.91
14.12
13.69
14.47
15.05
1.94
21.1
16.01
15.24
14.26
14.58
15.02
0.78
22.4
15.85
16.13
14.28
14.54
15.20
0.93
23.6
18.23
15.33
14.68
15.49
15.93
1.57
24.9
20.12
19.45
16.14
16.60
18.08
2.00
26.1
21.17
19.41
19.46
18.32
19.59
1.18
27.4
18.52
18.96
20.24
19.23
19.24
0.73
28.6
19.45
20.08
17.71
18.51
18.94
1.04
29.9
18.39
17.21
16.81
16.76
17.29
0.76
31.1
19.29
17.83
17.23
16.41
17.69
1.22
32.4
18.53
15.12
16.72
16.89
16.81
1.39
33.6
18.69
16.53
17.36
15.99
17.14
1.18
34.9
18.84
16.51
16.59
15.65
16.90
1.36
36.1
18.59
14.60
15.98
14.62
15.95
1.88
37.4
17.62
14.68
16.18
15.30
15.94
1.27
38.6
16.44
14.48
14.14
15.27
15.08
1.02
39.9
16.15
12.94
14.29
15.48
14.72
1.41
41.1
15.21
13.64
14.29
14.75
14.47
0.67
42.4
15.50
15.37
13.42
14.02
14.58
1.02
43.6
15.94
13.52
15.32
15.22
15.00
1.04
44.9
16.36
13.59
14.18
14.09
14.56
1.23
46.1
16.01
14.15
14.92
14.94
15.00
0.76
47.4
16.00
14.11
14.52
14.85
14.87
0.81
48.6
18.97
15.99
17.26
15.91
17.03
1.43
49.9
21.66
18.42
17.93
17.63
18.91
1.86
51.1
18.81
19.72
18.76
18.78
19.02
0.47
52.4
19.91
19.11
17.76
17.42
18.55
1.16
53.6
18.13
18.31
17.13
17.61
17.79
0.53
54.9
19.04
16.79
16.98
16.20
17.25
1.24
56.1
19.21
15.17
17.38
16.28
17.01
1.72
57.4
18.16
16.35
17.05
16.49
17.01
0.82
58.6
18.42
16.12
16.12
16.24
16.73
1.13
59.9
18.84
16.11
16.36
16.05
16.84
1.34
61.1
17.44
15.08
15.28
15.33
15.78
1.11
62.4
17.66
15.22
14.46
15.22
15.64
1.39
63.6
15.27
13.11
14.82
13.76
14.24
0.98
64.9
15.52
14.03
13.04
14.49
14.27
1.03
66.1
14.25
12.15
13.77
13.48
13.41
0.90
67.4
15.72
14.09
13.50
12.84
14.04
1.23
68.6
15.34
12.66
13.55
13.02
13.64
1.19
69.9
15.08
14.14
12.63
14.25
14.02
1.02
71.1
14.87
13.67
12.91
13.09
13.64
0.89
72.4
17.36
13.95
14.19
14.87
15.09
1.56
73.6
18.36
17.87
15.30
15.80
16.83
1.51
74.9
18.82
16.27
16.93
17.77
17.45
1.10
76.1
17.86
17.62
16.97
18.62
17.76
0.68
77.4
18.01
17.66
16.20
17.84
17.43
0.83
Page A51 of A172
Appendix to TNO-report of 3M-02
Final version
Time (hr) 6.1 7.4 8.6 9.9 11.1 12.4 13.6 14.9 16.1 17.4 18.6 19.9 21.1 22.4 23.6 24.9 26.1 27.4 28.6 29.9 31.1 32.4 33.6 34.9 36.1 37.4 38.6 39.9 41.1 42.4 43.6 44.9 46.1 47.4 48.6 49.9 51.1 52.4 53.6 54.9 56.1 57.4 58.6 59.9 61.1 62.4 63.6 64.9 66.1 67.4 68.6 69.9 71.1 72.4 73.6 74.9 76.1 77.4
group 1 vs 3 nn p 4 4 0.886 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.343 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.200 4 4 1.000 4 4 0.886 4 4 0.343 4 4 0.200 4 4 0.343 4 4 1.000 4 4 0.886 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.029 4 4 0.686 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.686 4 4 1.000 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.057 4 4 0.343 4 4 0.686 4 4 0.886 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.200 4 4 0.886 4 4 0.886 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.686 4 4 1.000 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.886
Energy expenditure, p-values Mann Whitney U-test group 1 vs 4 group 1 vs 6 group 3 vs 4 group 3 vs 6 group 4 vs 6 nn p nn p nn p nn p nn p 4 4 0.057 4 4 0.057 4 4 0.029 4 4 0.029 4 4 0.200 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.029 4 4 0.114 4 4 0.114 4 4 0.114 4 4 0.343 4 4 0.114 4 4 0.686 4 4 0.886 4 4 0.200 4 4 0.486 4 4 0.057 4 4 0.200 4 4 0.114 4 4 0.114 4 4 0.886 4 4 0.343 4 4 0.486 4 4 0.057 4 4 0.114 4 4 0.200 4 4 0.200 4 4 0.343 4 4 0.200 4 4 0.057 4 4 0.686 4 4 0.343 4 4 0.343 4 4 0.029 4 4 0.029 4 4 0.114 4 4 0.057 4 4 0.343 4 4 0.029 4 4 0.029 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.114 4 4 0.200 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.114 4 4 0.343 4 4 0.200 4 4 0.343 4 4 0.343 4 4 0.886 4 4 0.114 4 4 0.200 4 4 0.686 4 4 0.114 4 4 0.057 4 4 0.486 4 4 0.343 4 4 0.686 4 4 0.029 4 4 0.029 4 4 0.114 4 4 0.114 4 4 0.686 4 4 0.029 4 4 0.029 4 4 0.686 4 4 0.686 4 4 0.886 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.029 4 4 0.057 4 4 0.029 4 4 0.486 4 4 0.343 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.343 4 4 0.114 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.486 4 4 0.114 4 4 0.029 4 4 0.886 4 4 0.114 4 4 0.686 4 4 0.114 4 4 0.057 4 4 0.200 4 4 0.114 4 4 0.343 4 4 0.886 4 4 0.200 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.114 4 4 0.200 4 4 0.057 4 4 0.343 4 4 0.686 4 4 0.057 4 4 0.686 4 4 0.200 4 4 0.200 4 4 0.057 4 4 0.114 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.200 4 4 0.114 4 4 0.686 4 4 0.486 4 4 0.886 4 4 0.200 4 4 0.057 4 4 0.486 4 4 0.200 4 4 0.486 4 4 0.200 4 4 0.200 4 4 0.486 4 4 0.343 4 4 0.686 4 4 0.200 4 4 0.057 4 4 0.486 4 4 0.200 4 4 1.000 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.686 4 4 0.343 4 4 0.029 4 4 0.029 4 4 1.000 4 4 0.343 4 4 0.114 4 4 0.200 4 4 0.200 4 4 0.686 4 4 0.343 4 4 0.886 4 4 0.057 4 4 0.200 4 4 0.686 4 4 0.200 4 4 0.486 4 4 0.686 4 4 0.114 4 4 0.114 4 4 0.886 4 4 1.000 4 4 0.486 4 4 0.486 4 4 0.886 4 4 0.886 4 4 0.886 4 4 0.057 4 4 0.114 4 4 0.486 4 4 0.886 4 4 0.686 4 4 0.029 4 4 0.029 4 4 0.200 4 4 0.029 4 4 0.486 4 4 0.057 4 4 0.029 4 4 0.029 4 4 0.029 4 4 0.886 4 4 0.029 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.886 4 4 0.343 4 4 0.057 4 4 0.486 4 4 0.200 4 4 0.486 4 4 0.114 4 4 0.114 4 4 0.343 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.029 4 4 0.686 4 4 0.057 4 4 0.200 4 4 0.200 4 4 0.029 4 4 0.686 4 4 0.200 4 4 0.200 4 4 0.686 4 4 0.029 4 4 0.686 4 4 0.200 4 4 0.210 4 4 0.686 4 4 0.029 4 4 0.886 4 4 0.029 4 4 0.200 4 4 0.114 4 4 0.029 4 4 0.114 4 4 0.029 4 4 0.114 4 4 0.686 4 4 0.114 4 4 0.486 4 4 0.200 4 4 0.686 4 4 0.343 4 4 0.200 4 4 0.486 4 4 0.486 4 4 0.486 4 4 0.200 4 4 0.057 4 4 0.200 4 4 0.114 4 4 0.886 4 4 0.686 4 4 0.200 4 4 0.686 4 4 0.486 4 4 0.343 4 4 0.057 4 4 0.057 4 4 0.486 4 4 0.200 4 4 0.486 4 4 0.029 4 4 0.114 4 4 0.114 4 4 0.057 4 4 0.686 4 4 0.343 4 4 0.486 4 4 0.686 4 4 0.886 4 4 1.000 4 4 0.686 4 4 0.343 4 4 0.686 4 4 0.686 4 4 0.486 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.686 4 4 0.114 4 4 0.029 4 4 0.200 4 4 0.029 4 4 0.200 4 4 0.200 4 4 0.200 4 4 0.029 4 4 0.343 4 4 0.029 4 4 0.486 4 4 0.200 4 4 0.114 4 4 0.343 4 4 0.114 4 4 0.686
04-12-2006
p<0.05
Page A52 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Appendix E. Fecal bile acid, sterol and fatty acid measurements
E1. Fecal weight measurements, bodyweight and food intake measurements
Bodyweight and food intake during collecting feces
Group#
1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7
Mouse#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70
cage#
1 1 2 2 3 3 4 5 5 5 6 6 6 7 8 8 8 9 9 10 11 12 13 13 14 15 15 15 16 17 18 18 19 19 19 19 20 20 21 21 22 22 22 22 23 24 25 25 26 27 28 29 29 30 30 31 31 31 31 32 33 34 35 36 36 37 38 38 39 39
eartag LR
01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
collection point 1; 2 days feces collection
start collection point 1
end collection point 1
bodyweight (g) food on cage (g) bodyweight (g) food off cage (g)
33.9
33.9
31.2
49.7
30.5
38.9
30.4
31.0
28.1
51.9
28.2
39.5
31.1
30.6
29.5
47.4
29.4
35.8
26.7
26.9
27.2
20.5
30.4
30.6
30.6
31.0
27.2
61.6
27.3
44.8
33.0
33.1
29.8
30.0
31.2
68.5
32.0
48.6
32.8
31.7
33.1
24.8
30.8
31.0
30.1
29.8
32.8
68.0
32.8
47.2
26.5
26.6
26.9
42.3
26.6
31.8
29.0
29.2
28.8
23.4
27.8
32.1
28.3
26.2
28.8
32.9
28.9
27.2
32.2
32.3
28.8
44.9
28.9
30.3
25.8
31.2
26.3
25.2
28.7
28.5
30.1
30.1
25.0
77.5
24.7
61.0
29.8
23.1
29.7
17.1
27.0
26.3
27.0
20.0
30.3
30.2
29.3
48.3
29.2
36.3
29.2
28.9
30.9
30.7
31.0
30.7
25.6
90.1
25.8
68.4
27.0
26.7
30.2
43.1
30.2
31.0
27.7
27.2
27.7
47.9
27.7
36.0
28.9
30.0
25.2
25.3
27.6
27.8
28.5
90.3
28.1
68.8
28.6
37.8
29.7
30.8
28.5
27.2
28.6
21.1
30.3
30.1
29.3
50.7
29.9
37.2
27.3
27.7
27.2
22.1
27.7
27.1
28.7
20.2
28.4
31.9
28.5
26.2
31.3
31.5
27.3
52.3
27.2
40.4
30.2
30.0
28.8
42.9
28.4
31.2
25.6
25.4
27.6
27.8
29.1
29.1
30.3
88.0
30.7
67.7
26.7
30.5
26.7
25.0
28.2
29.1
27.7
23.4
27.3
31.2
26.7
25.5
26.2
34.7
26.6
29.3
28.2
28.5
27.4
21.2
28.2
9.4
26.1
26.4
26.1
21.3
26.0
26.2
26.7
53.0
26.9
42.5
23.6
23.0
25.1
43.8
24.6
32.4
Average bodyweight (g)
33.90 30.85 30.70 28.15 30.85 29.45 26.95 30.50 30.80 27.25
33.05 29.90 31.60 32.95 30.90 29.95 32.80 26.55 26.75 28.90
28.05 28.85 32.25 28.85 26.05 28.60 30.10 24.85 29.75 27.00
30.25 29.25 29.05 30.80 30.85 25.70 26.85 30.20 27.45 27.70
29.45 25.25 27.70 28.30 29.15 28.55 30.20 29.60 27.25 28.20
28.45 31.40 27.25 30.10 28.60 25.50 27.70 29.10 30.50 26.70
27.95 27.00 26.40 28.35 27.80 26.10 26.10 26.80 23.30 24.85
Food intake (g) coll. point 1
10.8 12.4 11.6 6.4
16.8
19.9 6.9
20.8 10.5 5.8 5.9 5.7 14.6 6.0
16.5 6.0 6.3
12.0
21.7 12.1
11.9
21.5 7.0 6.1 13.5 5.6 6.9 5.7 11.9 11.7
20.3 5.5 5.7 5.7 5.4 11.8 5.1 10.5
11.4
Page A53 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group#
1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7
Mouse#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70
cage#
1 1 2 2 3 3 4 5 5 5 6 6 6 7 8 8 8 9 9 10 11 12 13 13 14 15 15 15 16 17 18 18 19 19 19 19 20 20 21 21 22 22 22 22 23 24 25 25 26 27 28 29 29 30 30 31 31 31 31 32 33 34 35 36 36 37 38 38 39 39
eartag LR
01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
collection point 2; 3 days feces collection
start collection point 2
end collection point 2
bodyweight (g) food on cage (g) bodyweight (g) food off cage (g)
33.9
32.9
30.5
38.9
30.1
22.1
31
30
28.2
39.5
28.3
19.5
30.6
29.2
29.4
35.8
30.4
16.8
27.2
20.5
26.3
10.7
30.6
30
31
30.5
27.3
44.8
27
19.2
33.1
32.2
30
30
32
48.6
31.2
19.8
33.1
24.8
33.6
12.4
31
30.1
29.8
30
32.8
47.2
32.5
14.2
26.6
26.3
26.6
31.8
26.9
13.6
28.8
23.4
29
13.4
28.3
26.2
28.4
15.7
28.9
27.2
29.2
16.2
32.3
32.3
28.9
30.3
29.2
7.1
26.3
25.2
26.1
15.6
28.5
28.8
30.1
29.8
24.7
61.0
25.1
32.7
29.7
17.1
29.4
8.0
27
20.0
27.6
9.4
30.2
29.6
29.2
36.3
28.7
16.5
28.9
29.6
30.7
30.5
30.7
30.7
25.8
68.4
26.2
33.2
26.7
26.9
30.2
31.0
30.1
11.1
27.2
27.2
27.7
36.0
27.7
17.7
30
29.1
25.3
25.5
27.8
28.6
28.1
68.8
28.8
32.3
29.7
30.8
30.1
20.1
28.6
21.1
28.6
10.6
30.1
30.1
29.9
37.2
29.7
15.5
27.2
22.1
27
12.5
28.7
20.2
28.4
10.2
28.5
26.2
28.7
16.6
31.5
31.4
27.2
40.4
27
20.3
30
29.7
28.4
31.2
28
12.3
25.4
25.3
27.8
26.9
29.1
28.8
30.7
67.7
30.9
36.7
26.7
25.0
26.3
16.0
27.7
23.4
29.1
12.7
26.7
25.5
27.8
16.3
26.6
29.3
26.8
20.0
28.5
28.9
28.2
56.1
27.6
38.8
26.1
21.3
26.2
12.1
26.2
26.7
26.9
42.5
27.2
24.9
23
23.6
24.6
32.4
25.7
15.0
Average bodyweight (g)
33.40 30.30 30.50 28.25 29.90 29.90 26.75 30.30 30.75 27.15
32.65 30.00 31.60 33.35 30.55 29.90 32.65 26.45 26.75 28.90
28.35 29.05 32.30 29.05 26.20 28.65 29.95 24.90 29.55 27.30
29.90 28.95 29.25 30.60 30.70 26.00 26.80 30.15 27.20 27.70
29.55 25.40 28.20 28.45 29.90 28.60 30.10 29.80 27.10 28.55
28.60 31.45 27.10 29.85 28.20 25.35 27.35 28.95 30.80 26.50
28.40 27.25 26.70 28.70 27.90 26.15 26.45 27.05 23.30 25.15
Food intake (g) coll. point 2
16.8 20.0 19.0 9.8
25.6
28.8 12.4
33.0 18.2 10.0 10.5 11.0 23.2 9.6
28.3 9.1 10.6
19.8
35.2 19.9
18.3
36.5 10.7 10.5 21.7 9.6 10.0 9.6 20.1 18.9
31.0 9.0 10.7 9.2 9.3 17.3 9.2 17.6
17.4
Page A54 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group#
1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7
Mouse#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70
cage#
1 1 2 2 3 3 4 5 5 5 6 6 6 7 8 8 8 9 9 10 11 12 13 13 14 15 15 15 16 17 18 18 19 19 19 19 20 20 21 21 22 22 22 22 23 24 25 25 26 27 28 29 29 30 30 31 31 31 31 32 33 34 35 36 36 37 38 38 39 39
eartag LR
01 12 00 20 00 02 11 00 02 12
10 21 02 01 10 01 20 00 21 10
01 02 01 11 10 01 20 12 02 10
10 02 00 10 01 02 00 11 00 11
10 01 11 12 12 20 01 11 00 12
01 10 12 10 02 00 01 02 22 21
20 10 02 00 12 00 00 20 10 01
collection point 3; 2 days feces collection
start collection point 3
end collection point 3
bodyweight (g) food on cage (g) bodyweight (g) food off cage (g)
32.9
33.2
30.1
78.3
30.2
68.3
30
30.2
28.3
73.4
28.4
63.2
29.2
31.1
30.4
67.0
29.6
56.6
26.3
38.3
26.5
33.4
30
30.8
30.5
30.7
27
94.8
27.1
81.0
32.2
32.8
30
30.0
31.2
97.1
31.8
80.4
33.6
42.0
33.1
36.8
30.1
30.3
30
30.9
32.5
85.6
32.7
67.2
26.3
25.9
26.9
60.9
26.5
52.8
29
46.3
29.1
41.2
28.4
39.9
29.1
34.3
29.2
40.9
29.2
35.5
32.3
32.5
29.2
56.5
29.3
43.8
26.1
46.1
26.4
41.0
28.8
29.1
29.8
30.2
25.1
114.5
25.4
100.9
29.4
34.9
29.6
29.9
27.6
37.9
27.8
33.3
29.6
29.9
28.7
61.5
29.2
50.2
29.6
29.8
30.5
30.5
30.7
31.1
26.2
130.6
26.5
111.9
26.9
28.0
30.1
70.0
30.9
58.0
27.2
27.5
27.7
67.9
27.5
57.3
29.1
29.8
25.5
25.6
28.6
29.2
28.8
138.4
29.1
119.7
30.1
52.3
29.6
47.7
28.6
37.3
28.6
31.9
30.1
30.6
29.7
74.9
30.2
52.3
27
39.9
27.3
35.1
28.4
35.4
28.6
30.3
28.7
44.3
28.7
38.8
31.4
32.1
27
66.8
27.4
55.8
29.7
30.2
28
60.0
28.5
49.9
25.3
25.6
26.9
27.2
28.8
28.6
30.9
142.0
30.6
124.9
26.3
46.7
26.6
41.8
29.1
35.7
28.5
30.7
27.8
42.4
27.8
37.5
26.8
46.1
27.0
40.3
28.9
28.7
27.6
90.6
28.2
81.1
26.2
39.7
27.1
34.2
26.7
26.7
27.2
76.5
27.3
66.5
23.6
23.8
25.7
65.9
25.4
56.1
Average bodyweight (g)
33.05 30.15 30.10 28.35 30.15 30.00 26.40 30.40 30.60 27.05
32.50 30.00 31.50 33.35 30.20 30.45 32.60 26.10 26.70 29.05
28.75 29.20 32.40 29.25 26.25 28.95 30.00 25.25 29.50 27.70
29.75 28.95 29.70 30.50 30.90 26.35 27.45 30.50 27.35 27.60
29.45 25.55 28.90 28.95 29.85 28.60 30.35 29.95 27.15 28.50
28.70 31.75 27.20 29.95 28.25 25.45 27.05 28.70 30.75 26.45
28.80 27.80 26.90 28.80 27.90 26.65 26.70 27.25 23.70 25.55
Food intake (g) coll. point 3
10.0 10.2 10.4 4.9
13.8
16.7 5.2
18.4 8.1 5.1 5.6 5.4 12.7 5.1
13.6 5.0 4.6
11.3
18.7 12.0
10.6
18.7 4.6 5.4 22.6 4.8 5.1 5.5 11.0 10.1
17.1 4.9 5.0 4.9 5.8 9.5 5.5 10.0
9.8
Page A55 of A172
Appendix to TNO-report of 3M-02
Final version
Fecal weight
Group
1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 5 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 7 7
cage#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Feces collection point 1; 2 days collection
number of Tube empty Tube full Tube full after freeze drying
mice
(g)
(g)
(g)
2
6.6630 8.3248
8.0444
2
6.6490 8.6605
8.3426
2
6.6221 8.6309
8.3229
1
6.6808 7.664
7.4697
3
6.6712 9.4474
8.9658
3
6.6634 9.8825
9.4253
1
6.6794 7.8244
7.6266
3
6.6207 10.2937
9.7316
2
6.6605 8.2584
8.0008
1
6.6630 7.5596
7.4162
1
6.6697 7.5244
7.3942
1
6.6594 7.5056
7.3806
2
6.6379 9.0681
8.6519
1
6.6624 7.5339
7.4049
3
6.6568 9.433
8.9562
1
6.6607 7.5433
7.3604
1
6.6312 7.6683
7.5344
2
6.6765 8.6953
8.3655
4
6.6311 10.3697
9.7743
2
6.6493 8.6134
8.2667
2
6.6603 8.6425
8.2974
4
6.6918 10.4134
9.8868
1
6.6683 7.7517
7.5567
1
6.7028 7.6752
7.5296
2
6.6868 9.2271
8.8008
1
6.6825 7.5842
7.456
1
6.6889 7.8388
7.6669
1
6.6648 7.6251
7.4945
2
6.6617 8.6518
8.3356
2
6.6503 8.6434
8.3383
4
6.6110 9.8507
9.3193
1
6.6541 7.4635
7.3215
1
6.6615 7.3485
7.2406
1
6.6589 7.5622
7.3939
1
6.6820 7.476
7.3376
2
6.6335 8.4094
8.0542
1
6.6727 7.4706
7.3318
2
6.6890 8.3375
8.054
2
6.6706 8.2396
7.9761
freeze dried feces (g) 1.3814 1.6936 1.7008 0.7889 2.2946 2.7619 0.9472 3.1109 1.3403 0.7532 0.7245 0.7212 2.0140 0.7425 2.2994 0.6997 0.9032 1.6890 3.1432 1.6174 1.6371 3.1950 0.8884 0.8268 2.1140 0.7735 0.9780 0.8297 1.6739 1.6880 2.7083 0.6674 0.5791 0.7350 0.6556 1.4207 0.6591 1.3650 1.3055
04-12-2006
Page A56 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group
1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 5 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 7 7
cage#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Feces collection point 2; 3 days collection
number of Tube empty Tube full Tube full after freeze drying
mice
(g)
(g)
(g)
2
6.6728
9.043
8.654
2
6.6313 9.7853
9.3421
2
6.6987 9.5813
9.188
1
6.6499 8.1391
7.9395
3
6.6805 10.6433
10.0911
3
6.6610 11.3687
10.736
1
6.6450
8.426
8.1553
3
6.6479 12.3927
11.5086
2
6.6676 9.2172
8.8482
1
6.6137 8.0501
7.8405
1
6.6564 8.1514
7.945
1
6.6806 8.3368
8.1005
2
6.6554 10.2976
9.7616
1
6.6905 8.1358
7.9299
3
6.6709 11.0804
10.4427
1
6.6500 8.0103
7.7806
1
6.6622 8.2678
8.0649
2
6.6790 9.8571
9.3923
4
6.6452 12.0852
11.2859
2
6.6370
9.933
9.4414
2
6.6464
9.514
9.0495
4
6.6068 12.3762
11.578
1
6.6411 8.2187
7.9954
1
6.6557 8.1263
7.9237
2
6.6822 10.3758
9.8975
1
6.6134 8.0183
7.792
1
6.6799 8.1596
7.914
1
6.6762 8.1471
7.9636
2
6.6989
9.824
9.3799
2
6.6732 9.5294
9.1304
4
6.6621 11.3048
10.6299
1
6.6310 7.9252
7.7404
1
6.6902 8.1846
7.96
1
6.6712 7.9152
7.7309
1
6.6337 7.9533
7.7519
2
6.6633 9.2718
8.8526
1
6.6324 7.8893
7.7077
2
6.6776 9.1802
8.8063
2
6.6697
9.253
8.8469
freeze dried feces (g)
1.9812 2.7108 2.4893 1.2896 3.4106
4.0750 1.5103 4.8607 2.1806 1.2268
1.2886 1.4199 3.1062 1.2394 3.7718 1.1306 1.4027
2.7133 4.6407 2.8044 2.4031
4.9712 1.3543 1.2680 3.2153 1.1786 1.2341
1.2874 2.6810 2.4572 3.9678 1.1094
1.2698 1.0597 1.1182 2.1893 1.0753 2.1287 2.1772
Page A57 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group
1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 5 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 7 7
cage#
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Feces collection point 3; 2 days collection
number of Tube empty Tube full Tube full after freeze drying
mice
(g)
(g)
(g)
2
6.6429 8.5821
8.2399
2
6.6725 8.9822
8.6509
2
6.6831 8.9939
8.653
1
6.6645 7.7309
7.5727
3
6.6432 9.9317
9.4021
3
6.6844 10.106
9.6123
1
6.6652 7.8515
7.6749
3
6.6453 10.6479
10.0341
2
6.6397 8.3585
8.1165
1
6.6569 7.7971
7.6376
1
6.6860 7.7179
7.5628
1
6.6590 7.7398
7.58
2
6.6605 9.3189
8.8877
1
6.6334 7.6444
7.4947
3
6.6582 9.6295
9.1847
1
6.6145 7.6351
7.4819
1
6.6692 7.7894
7.631
2
6.6935 9.0966
8.7345
4
6.6306 10.6276
10.077
2
6.6585 9.1542
8.7793
2
6.6901 8.8739
8.5242
4
6.6840 10.9177
10.2953
1
6.6687 7.6866
7.5361
1
6.6635 7.8102
7.6563
2
6.6833
9.43
9.0427
1
6.6333 7.6942
7.5479
1
6.6752 7.7479
7.5966
1
6.6351 7.7734
7.6365
2
6.6633 9.0885
8.5695
2
6.6611 8.8705
8.5556
4
6.6657 10.3688
9.9106
1
6.6786 7.7434
7.5484
1
6.6860 7.6314
7.485
1
6.6887 7.5625
7.393
1
6.6783 7.9028
7.6867
2
6.6551
8.561
8.2858
1
6.6481
7.617
7.4543
2
6.6522 8.6462
8.3568
2
6.6641 8.7084
8.3702
freeze dried feces (g)
1.5970 1.9784 1.9699 0.9082 2.7589
2.9279 1.0097 3.3888 1.4768 0.9807
0.8768 0.9210 2.2272 0.8613 2.5265 0.8674 0.9618
2.0410 3.4464 2.1208 1.8341
3.6113 0.8674 0.9928 2.3594 0.9146 0.9214
1.0014 1.9062 1.8945 3.2449 0.8698
0.7990 0.7043 1.0084 1.6307 0.8062 1.7046 1.7061
Page A58 of A172
Appendix to TNO-report of 3M-02
Final version
Feces weight, bodyweight and food intake GC samples
GC-1
code 1:1-1 1:1-2 1:1-3 1:2-4 1:2-5 1:2-6 1:3-7 1:3-8 1:3-9 1:4-10 1:4-11 1:4-12 1:5-13 1:5-14 1:5-15 1:6-16 1:6-17 1:6-18 1:7-19 1:7-20 1:7-21
Feces collection 3M-02 week 6; collection point 1, 2 days collection
Group
cage# feces collected Bodyweight Food intake
(g)
(g)
(g)
1
1,2
3.075
123.6
23.2
1
3,4
2.4897
87.25
18
1
5
2.2946
88.55
16.8
2
6
2.7619
94.55
19.9
2
7,8
4.0581
126.6
27.7
2
9,10
2.0935
82.2
16.3
3
11,12,13
3.4597
118
26.2
3
15
2.2994
83.55
16.5
3
14,16,17
2.3454
82.8
18.3
4
18
1.689
59.5
12
4
19
3.1432
116.4
21.7
4
20,21
3.2545
112.2
24
5
22
3.195
110.7
21.5
5
24,25
2.9408
88.35
19.6
5
23,26,27
2.6399
84.6
19.5
6
28,29
2.5036
87.1
17.6
6
31
2.7083
112.8
20.3
6
30,32
2.3554
85.4
17.2
7
33,34,35
1.9697
81.35
16.8
7
36,37
2.0798
82.25
16.9
7
38,39
2.6705
101.05
21.9
GC-2
code 2:1-1 2:1-2 2:1-3 2:2-4 2:2-5 2:2-6 2:3-7 2:3-8 2:3-9 2:4-10 2:4-11 2:4-12 2:5-13 2:5-14 2:5-15 2:6-16 2:6-17 2:6-18 2:7-19 2:7-20 2:7-21
Feces collection 3M-02 week 6; collection point 2, 3 days collection
Group
cage# feces collected Bodyweight Food intake
(g)
(g)
(g)
1
1,2
4.692
122.45
36.8
1
3,4
3.7789
86.55
28.8
1
5
3.4106
88.2
25.6
2
6
4.075
94.25
28.8
2
7,8
6.371
126.45
45.4
2
9,10
3.4074
82.1
28.2
3
11,12,13
5.8147
118.75
44.7
3
15
3.7718
83.5
28.3
3
14,16,17
3.7727
83.05
29.3
4
18
2.7133
58.85
19.8
4
19
4.6407
116.55
35.2
4
20,21
5.2075
111.85
38.2
5
22
4.9712
111.6
36.5
5
24,25
4.4833
88.5
32.2
5
23,26,27
3.767
85.55
30.3
6
28,29
3.9684
87.15
29.7
6
31
3.9678
112.45
31
6
30,32
3.5666
84.55
27.9
7
33,34,35
3.4477
82.35
29.2
7
36,37
3.2646
82.75
26.5
7
38,39
4.3059
101.95
35
GC-3
code 3:1-1 3:1-2 3:1-3 3:2-4 3:2-5 3:2-6 3:3-7 3:3-8 3:3-9 3:4-10 3:4-11 3:4-12 3:5-13 3:5-14 3:5-15 3:6-16 3:6-17 3:6-18 3:7-19 3:7-20 3:7-21
Feces collection 3M-02 week 6; collection point 3, 2 days collection
Group cage# feces collected Bodyweight Food intake
(g)
(g)
(g)
1
1,2
3.5754
121.65
20.2
1
3,4
2.8781
86.55
15.3
1
5
2.7589
88.05
13.8
2
6
2.9279
94
16.7
2
7,8
4.3985
126.6
23.6
2
9,10
2.4575
81.85
13.2
3
11,12,13
4.025
119.6
23.7
3
15
2.5265
84.2
13.6
3
14,16,17
2.6905
83.45
14.7
4
18
2.041
58.7
11.3
4
19
3.4464
117.45
18.7
4
20,21
3.9549
112.9
22.6
5
22
3.6113
112.85
18.7
5
24,25
3.3522
88.9
28
5
23,26,27
2.7034
85.5
14.5
6
28,29
2.9076
87.65
16.5
6
31
3.2449
111.95
17.1
6
30,32
2.7643
84.65
15
7
33,34,35
2.5117
83.5
15.7
7
36,37
2.4369
83.35
15
7
38,39
3.4107
103.2
19.8
04-12-2006
Page A59 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
E2. Fecal bile acid measurements
1:1-1 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
5.8
area
770 2880 837 1412 3635 3854 411
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
1062.6 2880 979 1412 3925.8 5704 411
% g bile acid ng bile acid/ nmol bile acid/
mg feces mg feces
6.5
0.447
77.1
0.189
17.6
1.211
208.9
0.532
6.0
0.412
71.0
0.174
8.6
0.594
102.4
0.272
24.0
1.651
284.7
0.697
34.8
2.399
413.6
1.012
2.5
0.173
29.8
0.076
total
13799
16375
100
7
1187
3.0
nor-hyodeoxy 23775
1
23775
23775
1:1-2 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
6
area
481 1551 1143 2203 2222 5717 261
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
663.78 1551 1337 2203 2399.76 8461 261
% g bile acid ng bile acid/ nmol bile acid/
mg feces mg feces
3.9
0.276
46.0
0.113
9.2
0.645
107.4
0.274
7.9
0.556
92.6
0.227
13.1
0.916
152.6
0.405
14.2
0.997
166.2
0.407
50.1
3.517
586.1
1.435
1.5
0.108
18.1
0.046
total
13578
16877
100
7
1169
2.9
nor-hyodeoxy 24059
1
24059
24059
1:1-3 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
5.5
area
304 1047 535 1334 1317 3031 192
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
419.52 1047 626 1334 1422.36 4486 192
% g bile acid ng bile acid/ nmol bile acid/
mg feces mg feces
4.4
0.269
48.9
0.120
11.0
0.672
122.1
0.311
6.6
0.401
73.0
0.179
14.0
0.856
155.6
0.413
14.9
0.912
165.9
0.406
47.1
2.877
523.1
1.280
2.0
0.123
22.4
0.057
total
7760
9527
100
6
1111
2.8
nor-hyodeoxy 15591
1
15591
15591
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A60 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:1-1 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:1-2 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:1-3 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
7.2
area
314 688 213 335 803 507
2860
4612
7.2
area
1014 2554 2363 2730 4506 9183 213
22563
29667
5.1
area
1430 3201 3202 808 5434 7559 255
21889
43696
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
433.32 688 249 335
867.24 750 0
3323
1
4612
%
13.0 20.7 7.5 10.1 26.1 22.6 0.0
100
4612
g bile acid ng bile acid/ nmol bile acid/
mg feces mg feces
0.940
130.5
0.319
1.492
207.2
0.528
0.540
75.0
0.184
0.726
100.9
0.268
1.880
261.2
0.639
1.627
226.0
0.553
0.000
0.0
0.000
7
1001
2.5
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1399.32
5.0
0.472
65.5
0.160
1
2554
9.1
0.861
119.6
0.305
1
2765
9.8
0.932
129.4
0.317
1
2730
9.7
0.920
127.8
0.339
1.08
4866.48
17.3
1.640
227.8
0.558
1
13591
48.3
4.581
636.3
1.557
1
213
0.8
0.072
10.0
0.025
28118
100
9
1316
3.3
1
29667
29667
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1973.4
7.3
0.452
88.6
0.217
1
3201
11.8
0.733
143.6
0.366
1
3746
13.9
0.857
168.1
0.411
1
808
3.0
0.185
36.3
0.096
1.08
5868.72
21.7
1.343
263.3
0.645
1
11187
41.4
2.560
502.0
1.229
1
255
0.9
0.058
11.4
0.029
27040
100
6
1213
3.0
1
43696
43696
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A61 of A172
Appendix to TNO-report of 3M-02
Final version
3:1-1 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:1-2 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:1-3 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
7
area
755 6854 1031 1211 6883 6151 349
23234
32626
6.6
area
475 1558 748 1489 1894 3897 198
10259
20195
6.1
area
750 1906 1166 974 3492 4213 396
12897
24103
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1041.9
3.8
0.319
45.6
0.112
1
6854
25.2
2.101
300.1
0.764
1
1206
4.4
0.370
52.8
0.129
1
1211
4.5
0.371
53.0
0.141
1.08
7433.64
27.3
2.278
325.5
0.797
1
9103
33.5
2.790
398.6
0.976
1
349
1.3
0.107
15.3
0.039
27199
100
8
1191
3.0
1
32626
32626
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
655.5
5.2
0.325
49.2
0.120
1
1558
12.4
0.771
116.9
0.298
1
875
7.0
0.433
65.7
0.161
1
1489
11.8
0.737
111.7
0.297
1.08
2045.52
16.2
1.013
153.5
0.376
1
5768
45.8
2.856
432.7
1.059
1
198
1.6
0.098
14.9
0.038
12589
100
6
944
2.3
1
20195
20195
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1035
6.6
0.429
70.4
0.172
1
1906
12.2
0.791
129.6
0.330
1
1364
8.7
0.566
92.8
0.227
1
974
6.2
0.404
66.2
0.176
1.08
3771.36
24.0
1.565
256.5
0.628
1
6235
39.8
2.587
424.1
1.038
1
396
2.5
0.164
26.9
0.069
15682
100
7
1067
2.6
1
24103
24103
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A62 of A172
Appendix to TNO-report of 3M-02
Final version
1:2-4 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:2-5 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:2-6 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
6.3
area
133 741 488 509 758 1756 133
4518
8688
6.4
area
385 1785 1437 1980 5071 4256 151
15065
25522
5.3
area
696 3055 1508 3344 2771 7511 363
19248
29599
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
183.54 741 571 509
818.64 2599 133
5555
1
8688
%
3.3 13.3 10.3 9.2 14.7 46.8 2.4
100
8688
g bile acid ng bile acid/ nmol bile acid/
mg feces mg feces
0.211
33.5
0.082
0.853
135.4
0.345
0.657
104.3
0.255
0.586
93.0
0.247
0.942
149.6
0.366
2.991
474.8
1.162
0.153
24.3
0.062
6
1015
2.5
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
531.3
3.0
0.208
32.5
0.080
1
1785
10.0
0.699
109.3
0.278
1
1681
9.4
0.659
102.9
0.252
1
1980
11.1
0.776
121.2
0.322
1.08
5476.68
30.6
2.146
335.3
0.821
1
6299
35.2
2.468
385.6
0.944
1
151
0.8
0.059
9.2
0.024
17904
100
7
1096
2.7
1
25522
25522
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
960.48
4.1
0.324
61.2
0.150
1
3055
12.9
1.032
194.7
0.496
1
1764
7.5
0.596
112.5
0.275
1
3344
14.2
1.130
213.2
0.566
1.08
2992.68
12.7
1.011
190.8
0.467
1
11116
47.1
3.756
708.6
1.734
1
363
1.5
0.123
23.1
0.059
23596
100
8
1504
3.7
1
29599
29599
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A63 of A172
Appendix to TNO-report of 3M-02
Final version
2:2-4 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:2-5 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:2-6 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
7.7
area
564 1799 924 1298 1925 3892
10402
15018
6.6
area
521 1246 771 1507 3321 3749 322
11437
18400
6.4
area
1341 4170 1935 3343 3407 9002 474
23672
29674
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
778.32
6.1
0.518
67.3
0.165
1
1799
14.1
1.198
155.6
0.396
1
1081
8.4
0.720
93.5
0.229
1
1298
10.1
0.864
112.2
0.298
1.08
2079
16.2
1.384
179.8
0.440
1
5760
45.0
3.836
498.1
1.219
1
0
0.0
0.000
0.0
0.000
12796
100
9
1107
2.7
1
15018
15018
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
718.98
5.2
0.391
59.2
0.145
1
1246
9.0
0.677
102.6
0.261
1
902
6.5
0.490
74.3
0.182
1
1507
10.9
0.819
124.1
0.330
1.08
3586.68
25.9
1.949
295.3
0.723
1
5549
40.1
3.016
456.9
1.118
1
322
2.3
0.175
26.5
0.068
13831
100
8
1139
2.8
1
18400
18400
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1850.58
6.4
0.624
97.4
0.238
1
4170
14.3
1.405
219.6
0.559
1
2264
7.8
0.763
119.2
0.292
1
3343
11.5
1.127
176.0
0.467
1.08
3679.56
12.6
1.240
193.7
0.474
1
13323
45.8
4.490
701.5
1.717
1
474
1.6
0.160
25.0
0.064
29104
100
10
1532
3.8
1
29674
29674
04-12-2006
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A64 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
3:2-4 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:2-5 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:2-6 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
6.4
area
469 1577 949 839 2511 4030 280
10655
16647
6.5
area
438 1197 858 906 3408 2988 364
10159
18855
5.5
area
871 2878 1791 1999 2933 6248 527
17247
26219
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
647.22
4.9
0.389
60.7
0.149
1
1577
12.0
0.947
148.0
0.377
1
1110
8.5
0.667
104.2
0.255
1
839
6.4
0.504
78.7
0.209
1.08
2711.88
20.7
1.629
254.5
0.623
1
5964
45.4
3.583
559.8
1.370
1
280
2.1
0.168
26.3
0.067
13130
100
8
1232
3.0
1
16647
16647
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
604.44
5.0
0.321
49.3
0.121
1
1197
9.8
0.635
97.7
0.249
1
1004
8.2
0.532
81.9
0.200
1
906
7.4
0.481
73.9
0.196
1.08
3680.64
30.2
1.952
300.3
0.735
1
4422
36.3
2.345
360.8
0.883
1
364
3.0
0.193
29.7
0.076
12178
100
6
994
2.5
1
18855
18855
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1201.98
5.7
0.458
83.4
0.204
1
2878
13.6
1.098
199.6
0.508
1
2095
9.9
0.799
145.3
0.356
1
1999
9.5
0.762
138.6
0.368
1.08
3167.64
15.0
1.208
219.7
0.538
1
9247
43.8
3.527
641.2
1.569
1
527
2.5
0.201
36.5
0.093
21116
100
8
1464
3.6
1
26219
26219
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A65 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:3-7 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:3-8 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:3-9 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5.8
area
463 2160 560 2503 3081 5475 269
14511
22598
5.4
area
258 1480 573 1667 1531 3397 212
9118
15759
6.4
area
483 1604 840 2300 2706 3466
11399
16088
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
638.94
3.6
0.283
48.7
0.119
1
2160
12.2
0.956
164.8
0.420
1
655
3.7
0.290
50.0
0.122
1
2503
14.2
1.108
191.0
0.507
1.08
3327.48
18.8
1.472
253.9
0.621
1
8103
45.9
3.586
618.2
1.513
1
269
1.5
0.119
20.5
0.052
17657
100
8
1347
3.4
1
22598
22598
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
356.04
3.2
0.226
41.8
0.102
1
1480
13.4
0.939
173.9
0.443
1
670
6.1
0.425
78.8
0.193
1
1667
15.1
1.058
195.9
0.520
1.08
1653.48
14.9
1.049
194.3
0.476
1
5028
45.4
3.190
590.8
1.446
1
212
1.9
0.135
24.9
0.063
11066
100
7
1300
3.2
1
15759
15759
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
666.54
4.9
0.414
64.7
0.158
1
1604
11.8
0.997
155.8
0.397
1
983
7.2
0.611
95.5
0.234
1
2300
16.9
1.430
223.4
0.593
1.08
2922.48
21.5
1.817
283.8
0.695
1
5130
37.7
3.189
498.2
1.219
1
0
0.0
0.000
0.0
0.000
13606
100
8
1321
3.3
1
16088
16088
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A66 of A172
Appendix to TNO-report of 3M-02
Final version
2:3-7 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:3-8 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:3-9 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5
area
213 1811 1070 2080 2392 4005 367
11938
21102
7.3
area
222 790 602 1050 1101 2234 135
6134
10229
5.3
area
887 2496 1947 482 4734 4874
15420
36744
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
293.94
2.1
0.139
27.9
0.068
1
1811
12.7
0.858
171.6
0.437
1
1252
8.7
0.593
118.7
0.290
1
2080
14.5
0.986
197.1
0.523
1.08
2583.36
18.0
1.224
244.8
0.599
1
5927
41.4
2.809
561.8
1.375
1
367
2.6
0.174
34.8
0.089
14315
100
7
1357
3.4
1
21102
21102
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
306.36
4.1
0.300
41.0
0.100
1
790
10.6
0.772
105.8
0.269
1
704
9.4
0.689
94.3
0.231
1
1050
14.0
1.026
140.6
0.373
1.08
1189.08
15.9
1.162
159.2
0.390
1
3306
44.2
3.232
442.8
1.084
1
135
1.8
0.132
18.1
0.046
7481
100
7
1002
2.5
1
10229
10229
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1224.06
6.5
0.333
62.9
0.154
1
2496
13.3
0.679
128.2
0.326
1
2278
12.1
0.620
117.0
0.286
1
482
2.6
0.131
24.8
0.066
1.08
5112.72
27.2
1.391
262.5
0.643
1
7214
38.4
1.963
370.4
0.907
1
0
0.0
0.000
0.0
0.000
18806
100
5
966
2.4
1
36744
36744
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A67 of A172
Appendix to TNO-report of 3M-02
Final version
3:3-7 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:3-8 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:3-9 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
8.2
area
727 3031 1265 1974 4421 5331 401
17150
26440
6.4
area
541 1842 998 1904 2601 4796 270
12952
28268
7
area
612 2141 1127 2027 3995 4490 224
14616
22149
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
1003.26
4.9
0.379
46.3
0.113
1
3031
14.7
1.146
139.8
0.356
1
1480
7.2
0.560
68.3
0.167
1
1974
9.6
0.747
91.0
0.242
1.08
4774.68
23.2
1.806
220.2
0.539
1
7890
38.4
2.984
363.9
0.891
1
401
2.0
0.152
18.5
0.047
20554
100
8
948
2.4
1
26440
26440
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
746.58
4.7
0.264
41.3
0.101
1
1842
11.6
0.652
101.8
0.259
1
1168
7.4
0.413
64.5
0.158
1
1904
12.0
0.674
105.2
0.279
1.08
2809.08
17.7
0.994
155.3
0.380
1
7098
44.8
2.511
392.3
0.960
1
270
1.7
0.096
14.9
0.038
15837
100
6
875
2.2
1
28268
28268
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
844.56
4.8
0.381
54.5
0.133
1
2141
12.2
0.967
138.1
0.352
1
1319
7.5
0.595
85.0
0.208
1
2027
11.6
0.915
130.7
0.347
1.08
4314.6
24.6
1.948
278.3
0.681
1
6645
37.9
3.000
428.6
1.049
1
224
1.3
0.101
14.4
0.037
17515
100
8
1130
2.8
1
22149
22149
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A68 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:4-10 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:4-11 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:4-12 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5.1
area
103 354 286 394 349 1185 235
2906
12494
4.8
area
276 396 184 214 555 896
2521
8389
6
area
320 852 475 954 954 2059
5614
11311
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
142.14
4.0
0.114
22.3
0.055
1
354
9.9
0.283
55.6
0.142
1
335
9.3
0.268
52.5
0.129
1
394
11.0
0.315
61.8
0.164
1.08
376.92
10.5
0.302
59.2
0.145
1
1754
48.8
1.404
275.2
0.674
1
235
6.5
0.188
36.9
0.094
3590
100
3
563
1.4
1
12494
12494
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
380.88 396 215 214 599.4 1326 0
3132
1
8389
%
12.2 12.6 6.9 6.8 19.1 42.3 0.0
100
8389
g bile acid
0.454 0.472 0.257 0.255 0.715 1.581 0.000
ng bile acid/ mg feces
94.6 98.3 53.5 53.1 148.9 329.3 0.0
nmol bile acid/ mg feces
0.231 0.250 0.131 0.141 0.364 0.806 0.000
4
778
1.9
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
441.6
6.4
0.390
65.1
0.159
1
852
12.4
0.753
125.5
0.320
1
556
8.1
0.491
81.9
0.200
1
954
13.9
0.843
140.6
0.373
1.08
1030.32
15.0
0.911
151.8
0.372
1
3047
44.3
2.694
449.0
1.099
1
0
0.0
0.000
0.0
0.000
6881
100
6
1014
2.5
1
11311
11311
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A69 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:4-10 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:4-11 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:4-12 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
6.2
area
361 1051 1247 1420 979 2197
7255
19330
6.6
area
842 1126 866 1217 2527 3746 481
10805
22800
7.6
area
543 902 1180 1019 1622 2624 206
8096
13502
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
498.18
5.7
0.258
41.6
0.102
1
1051
12.0
0.544
87.7
0.223
1
1459
16.7
0.755
121.7
0.298
1
1420
16.3
0.735
118.5
0.315
1.08
1057.32
12.1
0.547
88.2
0.216
1
3252
37.2
1.682
271.3
0.664
1
0
0.0
0.000
0.0
0.000
8737
100
5
729
1.8
1
19330
19330
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
1161.96
8.8
0.510
77.2
0.189
1
1126
8.5
0.494
74.8
0.191
1
1013
7.6
0.444
67.3
0.165
1
1217
9.2
0.534
80.9
0.215
1.08
2729.16
20.6
1.197
181.4
0.444
1
5544
41.8
2.432
368.4
0.902
1
481
3.6
0.211
32.0
0.081
13272
100
6
882
2.2
1
22800
22800
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
749.34
7.6
0.555
73.0
0.179
1
902
9.1
0.668
87.9
0.224
1
1381
14.0
1.023
134.5
0.329
1
1019
10.3
0.755
99.3
0.264
1.08
1751.76
17.7
1.297
170.7
0.418
1
3884
39.3
2.876
378.5
0.926
1
206
2.1
0.153
20.1
0.051
9892
100
7
964
2.4
1
13502
13502
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A70 of A172
Appendix to TNO-report of 3M-02
Final version
3:4-10 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:4-11 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:4-12 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
8.1
area
374 928 737 749 1189 2355 244
6576
16790
8.3
area
474 1184 335 668 2030 2413 257
7361
13770
6.6
area
436 831 1182 693 1774 2161 172
7249
15281
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
516.12
6.4
0.307
38.0
0.093
1
928
11.5
0.553
68.2
0.174
1
862
10.7
0.514
63.4
0.155
1
749
9.3
0.446
55.1
0.146
1.08
1284.12
15.9
0.765
94.4
0.231
1
3485
43.2
2.076
256.3
0.627
1
244
3.0
0.145
17.9
0.046
8069
100
5
593
1.5
1
16790
16790
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
654.12
7.3
0.475
57.2
0.140
1
1184
13.3
0.860
103.6
0.264
1
392
4.4
0.285
34.3
0.084
1
668
7.5
0.485
58.4
0.155
1.08
2192.4
24.6
1.592
191.8
0.469
1
3571
40.0
2.593
312.5
0.765
1
257
2.9
0.187
22.5
0.057
8919
100
6
780
1.9
1
13770
13770
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces mg feces
1.38
601.68
6.8
0.394
59.7
0.146
1
831
9.4
0.544
82.4
0.210
1
1383
15.7
0.905
137.1
0.336
1
693
7.9
0.454
68.7
0.182
1.08
1915.92
21.8
1.254
190.0
0.465
1
3198
36.4
2.093
317.1
0.776
1
172
2.0
0.113
17.1
0.043
8795
100
6
872
2.2
1
15281
15281
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A71 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:5-13 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:5-14 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:5-15 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5.1
area
279 1398 233 1493 1698 1962 138
7201
19809
5.9
area
273 1027 1007 1318 1369 1924 193
7111
18666
6.6
area
201 976 457 1197 374 2027
5232
11586
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
385.02
4.6
0.194
38.1
0.093
1
1398
16.6
0.706
138.4
0.352
1
273
3.2
0.138
27.0
0.066
1
1493
17.7
0.754
147.8
0.392
1.08
1833.84
21.8
0.926
181.5
0.444
1
2904
34.5
1.466
287.4
0.703
1
138
1.6
0.070
13.7
0.035
8424
100
4
834
2.1
1
19809
19809
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
376.74
4.5
0.202
34.2
0.084
1
1027
12.2
0.550
93.3
0.238
1
1178
14.0
0.631
107.0
0.262
1
1318
15.7
0.706
119.7
0.318
1.08
1478.52
17.6
0.792
134.3
0.329
1
2848
33.8
1.526
258.6
0.633
1
193
2.3
0.103
17.5
0.045
8419
100
5
764
1.9
1
18666
18666
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
277.38
4.3
0.239
36.3
0.089
1
976
15.3
0.842
127.6
0.325
1
535
8.4
0.461
69.9
0.171
1
1197
18.7
1.033
156.5
0.416
1.08
403.92
6.3
0.349
52.8
0.129
1
3000
47.0
2.589
392.3
0.960
1
0
0.0
0.000
0.0
0.000
6389
100
6
836
2.1
1
11586
11586
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A72 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:5-13 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:5-14 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:5-15 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
6.9
area
170 421 188 1014 816 778 196
3583
8118
7.1
area
488 1599 1339 1652 2345 2835 357
10615
19702
5.4
area
1147 3831 1371 3141 2207 7603 531
19831
34357
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
234.6 421 220 1014 881.28 1151 196
4118
1
8118
%
5.7 10.2 5.3 24.6 21.4 28.0 4.8
100
8118
g bile acid
0.289 0.519 0.271 1.249 1.086 1.418 0.241
ng bile acid/ mg feces
41.9 75.2 39.3 181.0 157.3 205.6 35.0
nmol bile acid/ mg feces
0.103 0.191 0.096 0.481 0.385 0.503 0.089
5
735
1.8
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
673.44
5.4
0.342
48.1
0.118
1
1599
12.7
0.812
114.3
0.291
1
1567
12.5
0.795
112.0
0.274
1
1652
13.1
0.838
118.1
0.314
1.08
2532.6
20.1
1.285
181.0
0.443
1
4196
33.4
2.130
299.9
0.734
1
357
2.8
0.181
25.5
0.065
12576
100
6
899
2.2
1
19702
19702
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
1582.86
6.5
0.461
85.3
0.209
1
3831
15.7
1.115
206.5
0.526
1
1604
6.6
0.467
86.5
0.212
1
3141
12.9
0.914
169.3
0.450
1.08
2383.56
9.8
0.694
128.5
0.314
1
11252
46.3
3.275
606.5
1.484
1
531
2.2
0.155
28.6
0.073
24326
100
7
1311
3.3
1
34357
34357
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A73 of A172
Appendix to TNO-report of 3M-02
Final version
3:5-13 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:5-14 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:5-15 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
7.9
area
608 1723 925 1896 2481 3000 400
11033
29729
8.1
area
391 1007 1069 799 1999 2126 548
7939
20411
7.3
area
908 3513 1190 2079 2885 6815 481
17871
34491
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
839.04
6.4
0.282
35.7
0.087
1
1723
13.2
0.580
73.4
0.187
1
1082
8.3
0.364
46.1
0.113
1
1896
14.5
0.638
80.7
0.214
1.08
2679.48
20.5
0.901
114.1
0.279
1
4440
34.0
1.493
189.0
0.463
1
400
3.1
0.135
17.0
0.043
13060
100
4
556
1.4
1
29729
29729
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
539.58
5.7
0.264
32.6
0.080
1
1007
10.7
0.493
60.9
0.155
1
1251
13.2
0.613
75.7
0.185
1
799
8.5
0.391
48.3
0.128
1.08
2158.92
22.8
1.058
130.6
0.320
1
3146
33.3
1.542
190.3
0.466
1
548
5.8
0.268
33.1
0.084
9450
100
5
572
1.4
1
20411
20411
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
1253.04
5.7
0.363
49.8
0.122
1
3513
16.0
1.019
139.5
0.355
1
1392
6.4
0.404
55.3
0.135
1
2079
9.5
0.603
82.6
0.219
1.08
3115.8
14.2
0.903
123.7
0.303
1
10086
46.0
2.924
400.6
0.980
1
481
2.2
0.139
19.1
0.049
21920
100
6
871
2.2
1
34491
34491
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A74 of A172
Appendix to TNO-report of 3M-02
Final version
1:6-16 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:6-17 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:6-18 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5.8
area
222 654 724 565 752 1281 123
4321
16312
6.2
area
199 543 411 518 383 1077 111
3242
10983
6.3
area
117 650 962 598 677 1575 174
4753
13455
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
306.36
5.9
0.188
32.4
0.079
1
654
12.6
0.401
69.1
0.176
1
847
16.3
0.519
89.5
0.219
1
565
10.9
0.346
59.7
0.159
1.08
812.16
15.6
0.498
85.8
0.210
1
1896
36.4
1.162
200.4
0.490
1
123
2.4
0.075
13.0
0.033
5203
100
3
550
1.4
1
16312
16312
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
274.62
7.0
0.250
40.3
0.099
1
543
13.8
0.494
79.7
0.203
1
481
12.2
0.438
70.6
0.173
1
518
13.2
0.472
76.1
0.202
1.08
413.64
10.5
0.377
60.7
0.149
1
1594
40.5
1.451
234.1
0.573
1
111
2.8
0.101
16.3
0.042
3935
100
4
578
1.4
1
10983
10983
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
161.46
2.8
0.120
19.0
0.047
1
650
11.3
0.483
76.7
0.195
1
1126
19.5
0.837
132.8
0.325
1
598
10.4
0.444
70.5
0.187
1.08
731.16
12.7
0.543
86.3
0.211
1
2331
40.4
1.732
275.0
0.673
1
174
3.0
0.129
20.5
0.052
5771
100
4
681
1.7
1
13455
13455
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A75 of A172
Appendix to TNO-report of 3M-02
Final version
2:6-16 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:6-17 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:6-18 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5.6
area
215 821 747 598 840 1244 226
4691
17522
5.5
area
196 966 220 486 177 733
2778
11207
7.4
area
553 1729 1319 1154 1500 3330 318
9903
20902
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
296.7
5.3
0.169
30.2
0.074
1
821
14.8
0.469
83.7
0.213
1
874
15.7
0.499
89.1
0.218
1
598
10.7
0.341
60.9
0.162
1.08
907.2
16.3
0.518
92.5
0.226
1
1841
33.1
1.051
187.6
0.459
1
226
4.1
0.129
23.0
0.059
5564
100
3
567
1.4
1
17522
17522
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
270.48
8.3
0.241
43.9
0.107
1
966
29.7
0.862
156.7
0.399
1
257
7.9
0.230
41.8
0.102
1
486
14.9
0.434
78.8
0.209
1.08
191.16
5.9
0.171
31.0
0.076
1
1085
33.3
0.968
176.0
0.431
1
0
0.0
0.000
0.0
0.000
3256
100
3
528
1.3
1
11207
11207
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
763.14
6.3
0.365
49.3
0.121
1
1729
14.3
0.827
111.8
0.285
1
1543
12.8
0.738
99.8
0.244
1
1154
9.6
0.552
74.6
0.198
1.08
1620
13.4
0.775
104.7
0.256
1
4928
40.9
2.358
318.6
0.780
1
318
2.6
0.152
20.6
0.052
12056
100
6
779
1.9
1
20902
20902
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A76 of A172
Appendix to TNO-report of 3M-02
Final version
3:6-16 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:6-17 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
3:6-18 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
7.4
area
621 1219 1628 685 1439 1866 253
7711
23222
8.1
area
829 1831 1171 653 1120 4227 295
10126
26821
7.7
area
441 1194 995 999 934 1799
6362
24030
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
856.98
9.3
0.369
49.9
0.122
1
1219
13.2
0.525
70.9
0.181
1
1905
20.6
0.820
110.8
0.271
1
685
7.4
0.295
39.9
0.106
1.08
1554.12
16.8
0.669
90.4
0.221
1
2762
29.9
1.189
160.7
0.393
1
253
2.7
0.109
14.7
0.038
9235
100
4
537
1.3
1
23222
23222
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
1144.02
9.0
0.427
52.7
0.129
1
1831
14.4
0.683
84.3
0.215
1
1370
10.7
0.511
63.1
0.154
1
653
5.1
0.243
30.1
0.080
1.08
1209.6
9.5
0.451
55.7
0.136
1
6256
49.0
2.332
288.0
0.705
1
295
2.3
0.110
13.6
0.035
12759
100
5
587
1.5
1
26821
26821
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
608.58
8.0
0.253
32.9
0.080
1
1194
15.6
0.497
64.5
0.164
1
1164
15.2
0.484
62.9
0.154
1
999
13.1
0.416
54.0
0.143
1.08
1008.72
13.2
0.420
54.5
0.133
1
2663
34.9
1.108
143.9
0.352
1
0
0.0
0.000
0.0
0.000
7637
100
3
413
1.0
1
24030
24030
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A77 of A172
Appendix to TNO-report of 3M-02
Final version
1:7-19 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:7-20 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
1:7-21 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5.2
area
493 1997 1424 376 1518 1175
6983
12241
5.8
area
602 895 817 302 659 977 267
4519
11457
5.4
area
512 1419 1545 649 893 912 204
6134
13452
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
680.34
8.4
0.556
106.9
0.262
1
1997
24.7
1.631
313.7
0.799
1
1666
20.6
1.361
261.7
0.641
1
376
4.6
0.307
59.1
0.157
1.08
1639.44
20.2
1.339
257.6
0.630
1
1739
21.5
1.421
273.2
0.669
1
0
0.0
0.000
0.0
0.000
8098
100
7
1272
3.2
1
12241
12241
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
830.76
15.4
0.725
125.0
0.306
1
895
16.5
0.781
134.7
0.343
1
956
17.7
0.834
143.8
0.352
1
302
5.6
0.264
45.4
0.121
1.08
711.72
13.2
0.621
107.1
0.262
1
1446
26.7
1.262
217.6
0.533
1
267
4.9
0.233
40.2
0.102
5408
100
5
814
2.0
1
11457
11457
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
706.56
10.0
0.525
97.3
0.238
1
1419
20.0
1.055
195.3
0.498
1
1808
25.5
1.344
248.8
0.609
1
649
9.1
0.482
89.3
0.237
1.08
964.44
13.6
0.717
132.8
0.325
1
1350
19.0
1.003
185.8
0.455
1
204
2.9
0.152
28.1
0.072
7100
100
5
977
2.4
1
13452
13452
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A78 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:7-19 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:7-20 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
2:7-21 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
5.5
area
216 631 176 110 543 386
2062
5496
5.9
area
872 3015 1451 382 2004 3004 653
11381
27158
5.3
area
665 3474 1201 397 1600 2024 155
9516
15683
mg
response factor
1.38 1 1 1
1.08 1 1
area corrected for response factor
298.08 631 206 110
586.44 571 0
2403
1
5496
%
12.4 26.3 8.6 4.6 24.4 23.8 0.0
100
5496
g bile acid
0.542 1.148 0.375 0.200 1.067 1.039 0.000
ng bile acid/ mg feces
98.6 208.7 68.1 36.4 194.0 189.0
0.0
nmol bile acid/ mg feces
0.241 0.532 0.167 0.097 0.475 0.463 0.000
4
795
2.0
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
1203.36
8.9
0.443
75.1
0.184
1
3015
22.2
1.110
188.2
0.479
1
1698
12.5
0.625
106.0
0.259
1
382
2.8
0.141
23.8
0.063
1.08
2164.32
16.0
0.797
135.1
0.331
1
4446
32.8
1.637
277.5
0.679
1
653
4.8
0.240
40.8
0.104
13561
100
5
846
2.1
1
27158
27158
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
917.7
8.3
0.585
110.4
0.270
1
3474
31.4
2.215
418.0
1.065
1
1405
12.7
0.896
169.1
0.414
1
397
3.6
0.253
47.8
0.127
1.08
1728
15.6
1.102
207.9
0.509
1
2996
27.1
1.910
360.4
0.882
1
155
1.4
0.099
18.6
0.047
11072
100
7
1332
3.3
1
15683
15683
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
Page A79 of A172
Appendix to TNO-report of 3M-02
Final version
3:7-19 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
6.7
area
535 850 877 325 1150 921 605
5263
11706
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
738.3
12.0
0.631
94.1
0.230
1
850
13.8
0.726
108.4
0.276
1
1026
16.7
0.877
130.8
0.320
1
325
5.3
0.278
41.4
0.110
1.08
1242
20.2
1.061
158.4
0.388
1
1363
22.2
1.164
173.8
0.425
1
605
9.8
0.517
77.1
0.196
6149
100
5
784
1.9
1
11706
11706
3:7-20
weight feces
6.5
mg
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
oppervlakte
290 571 462 258 300 422
response factor
1.38 1 1 1
1.08 1 1
total
2303
nor-hyodeoxy 4214
1
oppervlakte
400.2 571 541 258 324 625
0
2718
4214
%
14.7 21.0 19.9 9.5 11.9 23.0 0.0
100
4214
g bile acid
0.950 1.355 1.283 0.612 0.769 1.482 0.000
ng bile acid/ mg faeces
146.1 208.5 197.3 94.2 118.3 228.0
0.0
nmol bile acid/ mg faeces
0.358 0.531 0.483 0.250 0.289 0.558 0.000
6
992
2.5
3:7-21 weight feces
a-muricholate deoxycholate
cholate lithocholate -muricholate w-muricholate hyodeo/urso
total
nor-hyodeoxy
7.2
area
1184 3208 2501 1182 3049 3105 1201
15430
32495
mg
response area corrected
% g bile acid ng bile acid/ nmol bile acid/
factor for response factor
mg feces
mg feces
1.38
1633.92
9.1
0.503
69.8
0.171
1
3208
17.8
0.987
137.1
0.349
1
2926
16.2
0.900
125.1
0.306
1
1182
6.6
0.364
50.5
0.134
1.08
3292.92
18.3
1.013
140.7
0.344
1
4595
25.5
1.414
196.4
0.481
1
1201
6.7
0.370
51.3
0.131
18039
100
6
771
1.9
1
32495
32495
a-muri deoxy cholate lithochol b-muri w-muri hyodeoxy/urso
408.6 392.6 408.6 376.6 408.6 408.6 392.6
04-12-2006
Page A80 of A172
Page A81 of A172
group
1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 AVERAGE SD
cage#
1,2 3,4 5 1,2 3,4 5 1,2 3,4 5
6 7,8 9,10 6 7,8 9,10 6 7,8 9,10
11,12,13 15
14,16,17 11,12,13
15 14,16,17 11,12,13
15 14,16,17
18 19 20,21 18 19 20,21 18 19 20,21
22 24,25 23,26,27
22 24,25 23,26,27
22 24,25 23,26,27
28,29 31
30,32 28,29
31 30,32 28,29
31 30,32
33,34,35 36,37 38,39
33,34,35 36,37 38,39
33,34,35 36,37 38,39
GC sample#
1:1-1 1:1-2 1:1-3 2:1-1 2:1-2 2:1-3 3:1-1 3:1-2 3:1-3
a-muricholate
0.189 0.113 0.120 0.319 0.160 0.217 0.112 0.120 0.172
deoxycholate
0.532 0.274 0.311 0.528 0.305 0.366 0.764 0.298 0.330
1:2-4 1:2-5 1:2-6 2:2-4 2:2-5 2:2-6 3:2-4 3:2-5 3:2-6
0.082 0.080 0.150 0.165 0.145 0.238 0.149 0.121 0.204
0.345 0.278 0.496 0.396 0.261 0.559 0.377 0.249 0.508
1:3-7 1:3-8 1:3-9 2:3-7 2:3-8 2:3-9 3:3-7 3:3-8 3:3-9
0.119 0.102 0.158 0.068 0.100 0.154 0.113 0.101 0.133
0.420 0.443 0.397 0.437 0.269 0.326 0.356 0.259 0.352
1:4-10 1:4-11 1:4-12 2:4-10 2:4-11 2:4-12 3:4-10 3:4-11 3:4-12
0.055 0.231 0.159 0.102 0.189 0.179 0.093 0.140 0.146
0.142 0.250 0.320 0.223 0.191 0.224 0.174 0.264 0.210
1:5-13 1:5-14 1:5-15 2:5-13 2:5-14 2:5-15 3:5-13 3:5-14 3:5-15
0.093 0.084 0.089 0.103 0.118 0.209 0.087 0.080 0.122
0.352 0.238 0.325 0.191 0.291 0.526 0.187 0.155 0.355
1:6-16 1:6-17 1:6-18 2:6-16 2:6-17 2:6-18 3:6-16 3:6-17 3:6-18
0.079 0.099 0.047 0.074 0.107 0.121 0.122 0.129 0.080
0.176 0.203 0.195 0.213 0.399 0.285 0.181 0.215 0.164
1:7-19 1:7-20 1:7-21 2:7-19 2:7-20 2:7-21 3:7-19 3:7-20 3:7-21
0.262 0.306 0.238 0.241 0.184 0.270 0.230 0.358 0.171
0.799 0.343 0.498 0.532 0.479 1.065 0.276 0.531 0.349
bile acids (mol/g feces)
cholate lithocholate b-muricholate w-muricholate hyodeo/urso
0.174
0.272
0.697
1.012
0.076
0.227
0.405
0.407
1.435
0.046
0.179
0.413
0.406
1.280
0.057
0.184
0.268
0.639
0.553
0.000
0.317
0.339
0.558
1.557
0.025
0.411
0.096
0.645
1.229
0.029
0.129
0.141
0.797
0.976
0.039
0.161
0.297
0.376
1.059
0.038
0.227
0.176
0.628
1.038
0.069
bile acids total (mol/g feces)
2.95 2.91 2.77 2.49 3.26 2.99 2.96 2.35 2.64
0.255
0.247
0.366
1.162
0.062
2.52
0.252
0.322
0.821
0.944
0.024
2.72
0.275
0.566
0.467
1.734
0.059
3.75
0.229
0.298
0.440
1.219
0.000
2.75
0.182
0.330
0.723
1.118
0.068
2.83
0.292
0.467
0.474
1.717
0.064
3.81
0.255
0.209
0.623
1.370
0.067
3.05
0.200
0.196
0.735
0.883
0.076
2.46
0.356
0.368
0.538
1.569
0.093
3.64
0.122
0.507
0.621
1.513
0.052
3.36
0.193
0.520
0.476
1.446
0.063
3.24
0.234
0.593
0.695
1.219
0.000
3.30
0.290
0.523
0.599
1.375
0.089
3.38
0.231
0.373
0.390
1.084
0.046
2.49
0.286
0.066
0.643
0.907
0.000
2.38
0.167
0.242
0.539
0.891
0.047
2.35
0.158
0.279
0.380
0.960
0.038
2.18
0.208
0.347
0.681
1.049
0.037
2.81
0.129
0.164
0.145
0.674
0.094
1.40
0.131
0.141
0.364
0.806
0.000
1.92
0.200
0.373
0.372
1.099
0.000
2.52
0.298
0.315
0.216
0.664
0.000
1.82
0.165
0.215
0.444
0.902
0.081
2.19
0.329
0.264
0.418
0.926
0.051
2.39
0.155
0.146
0.231
0.627
0.046
1.47
0.084
0.155
0.469
0.765
0.057
1.93
0.336
0.182
0.465
0.776
0.043
2.16
0.066
0.392
0.444
0.703
0.035
2.09
0.262
0.318
0.329
0.633
0.045
1.91
0.171
0.416
0.129
0.960
0.000
2.09
0.096
0.481
0.385
0.503
0.089
1.85
0.274
0.314
0.443
0.734
0.065
2.24
0.212
0.450
0.314
1.484
0.073
3.27
0.113
0.214
0.279
0.463
0.043
1.39
0.185
0.128
0.320
0.466
0.084
1.42
0.135
0.219
0.303
0.980
0.049
2.16
0.219
0.159
0.210
0.490
0.033
1.37
0.173
0.202
0.149
0.573
0.042
1.44
0.325
0.187
0.211
0.673
0.052
1.69
0.218
0.162
0.226
0.459
0.059
1.41
0.102
0.209
0.076
0.431
0.000
1.32
0.244
0.198
0.256
0.780
0.052
1.94
0.271
0.106
0.221
0.393
0.038
1.33
0.154
0.080
0.136
0.705
0.035
1.45
0.154
0.143
0.133
0.352
0.000
1.03
0.641
0.157
0.630
0.669
0.000
3.16
0.352
0.121
0.262
0.533
0.102
2.02
0.609
0.237
0.325
0.455
0.072
2.43
0.167
0.097
0.475
0.463
0.000
1.97
0.259
0.063
0.331
0.679
0.104
2.10
0.414
0.127
0.509
0.882
0.047
3.31
0.320
0.110
0.388
0.425
0.196
1.95
0.483
0.250
0.289
0.558
0.000
2.47
0.306
0.134
0.344
0.481
0.131
1.92
total feces (g)
3.075 2.4897 2.2946 4.692 3.7789 3.4106 3.5754 2.8781 2.7589
2.7619 4.0581 2.0935 4.075 6.371 3.4074 2.9279 4.3985 2.4575
3.4597 2.2994 2.3454 5.8147 3.7718 3.7727 4.025 2.5265 2.6905
1.689 3.1432 3.2545 2.7133 4.6407 5.2075 2.041 3.4464 3.9549
3.195 2.9408 2.6399 4.9712 4.4833 3.767 3.6113 3.3522 2.7034
2.5036 2.7083 2.3554 3.9684 3.9678 3.5666 2.9076 3.2449 2.7643
1.9697 2.0798 2.6705 3.4477 3.2646 4.3059 2.5117 2.4369 3.4107
average weight days
(g)
123.6
2
87.25
2
88.55
2
122.45
3
86.55
3
88.2
3
121.65
2
86.55
2
88.05
2
94.55
2
126.6
2
82.2
2
94.25
3
126.45
3
82.1
3
94
2
126.6
2
81.85
2
118
2
83.55
2
82.8
2
118.75
3
83.5
3
83.05
3
119.6
2
84.2
2
83.45
2
59.5
2
116.4
2
112.2
2
58.85
3
116.55
3
111.85
3
58.7
2
117.45
2
112.9
2
110.7
2
88.35
2
84.6
2
111.6
3
88.5
3
85.55
3
112.85
2
88.9
2
85.5
2
87.1
2
112.8
2
85.4
2
87.15
3
112.45
3
84.55
3
87.65
2
111.95
2
84.65
2
81.35
2
82.25
2
101.05
2
82.35
3
82.75
3
101.95
3
83.5
2
83.35
2
103.2
2
total bile acid excretion (mol/100 gr mouse/day)
3.67 4.15 3.58 3.18 4.75 3.86 4.35 3.90 4.14 3.95 0.46 3.68 4.36 4.77 3.96 4.75 5.27 4.75 4.27 5.46 4.59 0.58 4.92 4.46 4.67 5.52 3.75 3.61 3.96 3.26 4.53 4.30 0.71
1.99 2.60 3.66 2.79 2.90 3.71 2.56 2.84 3.78 2.98 0.61
3.01 3.17 3.26 2.74 3.78 4.80 2.22 2.67 3.42 3.23 0.74
1.96 1.73 2.33 2.14 1.56 2.72 2.21 2.11 1.68
2.05 0.36
3.82 2.55 3.22 2.75 2.76 4.67 2.93 3.61 3.17
3.27 0.67
a-muricholate
6.39 3.87 4.33 12.82 4.92 7.24 3.78 5.13 6.53
6.11 2.80
3.26 2.93 4.00 6.00 5.13 6.26 4.87 4.91 5.61
4.77 1.16
3.56 3.16 4.81 2.02 4.03 6.46 4.81 4.64 4.75
4.25 1.26
3.90 12.03 6.31 5.60 8.64 7.48 6.31 7.24 6.76
7.14 2.26
4.47 4.39 4.25 5.55 5.26 6.39 6.31 5.63 5.63
5.32 0.80
5.80 6.86 2.76 5.24 8.11 6.24 9.16 8.87 7.83
6.76 2.02
8.29 15.16 9.78 12.23 8.76 8.15 11.84 14.48 8.92
10.84 2.69
deoxycholate
18.03 9.42 11.24 21.19 9.34 12.23 25.85 12.68 12.51
14.72 5.71
13.69 10.23 13.24 14.43 9.25 14.67 12.36 10.11 13.98
12.44 2.06
12.51 13.66 12.04 12.93 10.81 13.71 15.12 11.92 12.53
12.80 1.25
10.10 13.02 12.67 12.29 8.72 9.37 11.81 13.64 9.72
11.26 1.80
16.89 12.46 15.55 10.36 13.00 16.10 13.48 10.94 16.43
13.91 2.43
12.88 14.11 11.55 15.10 30.13 14.70 13.56 14.77 15.99
15.87 5.51
25.31 16.99 20.45 26.94 22.83 32.13 14.19 21.50 18.23
22.06 5.50
cholate
5.89 7.80 6.46 7.37 9.71 13.75 4.37 6.84 8.60
7.87 2.69
10.13 9.26 7.35 8.33 6.43 7.65 8.36 8.15 9.78
8.38 1.19
3.65 5.94 7.09 8.59 9.26 12.02 7.09 7.26 7.41
7.59 2.30
9.17 6.80 7.94 16.39 7.54 13.77 10.54 4.34 15.55
10.23 4.17
3.16 13.73 8.19 5.20 12.24 6.48 8.13 13.05 6.25
8.49 3.72
16.03 12.00 19.22 15.45 7.71 12.61 20.37 10.62 14.98
14.33 4.04
20.29 17.44 25.03 8.45 12.35 12.49 16.45 19.56 15.97
16.45 4.95
% of bile acids
lithocholate b-muricholate
9.21
23.61
13.95
14.00
14.94
14.68
10.75
25.66
10.41
17.10
3.22
21.54
4.76
26.94
12.63
16.00
6.66
23.78
9.61
20.37
4.05
4.97
9.80
14.53
11.84
30.17
15.11
12.46
10.85
16.02
11.66
25.58
12.26
12.44
6.86
20.43
7.98
29.88
10.12
14.78
10.72
19.59
2.43
7.23
15.11
18.52
16.04
14.66
18.00
21.08
15.48
17.72
14.97
15.63
2.76
26.98
10.27
22.89
12.84
17.46
12.37
24.26
13.09
19.91
4.49
4.15
11.72
10.33
7.33
18.93
14.79
14.73
17.31
11.88
9.82
20.30
11.03
17.48
9.93
15.70
8.02
24.27
8.45
21.54
10.94
17.24
3.28
4.55
18.81
21.29
16.67
17.23
19.89
6.19
26.01
20.84
14.01
19.79
13.76
9.62
15.46
20.14
9.05
22.53
10.13
14.00
15.97
16.85
5.19
5.72
11.60
15.37
14.03
10.33
11.08
12.49
11.47
16.04
15.80
5.73
10.23
13.24
7.95
16.62
5.49
9.38
13.95
12.98
11.29
12.46
3.18
3.51
4.97
19.97
5.98
12.98
9.75
13.35
4.90
24.06
3.02
15.75
3.83
15.35
5.65
19.92
10.13
11.72
7.00
17.97
6.14
16.79
2.45
4.02
w-muricholate
34.30 49.37 46.29 22.20 47.75 41.06 32.99 45.11 39.32
39.82 8.75
46.13 34.70 46.28 44.38 39.57 45.04 44.92 35.90 43.16
42.23 4.42
45.10 44.58 36.99 40.65 43.46 38.07 37.82 44.13 37.37
40.91 3.42
48.08 41.89 43.55 36.53 41.25 38.74 42.61 39.53 35.96
40.90 3.75
33.71 33.19 45.94 27.22 32.79 45.43 33.36 32.84 45.31
36.64 6.96
35.89 39.79 39.81 32.54 32.51 40.27 29.53 48.49 34.26
37.01 5.73
21.18 26.38 18.69 23.43 32.35 26.62 21.86 22.60 25.08
24.24 3.97
hyodeo/urso
2.57 1.58 2.06 0.00 0.78 0.97 1.32 1.61 2.60
1.50 0.85
2.46 0.87 1.57 0.00 2.39 1.67 2.19 3.08 2.56
1.86 0.95
1.56 1.96 0.00 2.62 1.85 0.00 2.00 1.75 1.31
1.45 0.90
6.70 0.00 0.00 0.00 3.72 2.14 3.10 2.96 2.01
2.29 2.19
1.67 2.34 0.00 4.82 2.90 2.23 3.13 5.95 2.25
2.81 1.74
2.42 2.88 3.09 4.16 0.00 2.70 2.82 2.38 0.00
2.27 1.39
0.00 5.07 2.94 0.00 4.95 1.43 10.10 0.00 6.82
3.48 3.54
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Appendix to TNO-report of 3M-02
Final version
04-12-2006
bile acid
total bile acids a-muricholate deoxycholate
cholate lithocholate b-muricholate w-muricholate hyodeo/urso
bile acid
total bile acids a-muricholate deoxycholate
cholate lithocholate b-muricholate w-muricholate hyodeo/urso
bile acid
total bile acids a-muricholate deoxycholate
cholate lithocholate b-muricholate w-muricholate hyodeo/urso
group 1 vs 2 nn p 9 9 0.019 9 9 0.258 9 9 0.863 9 9 0.340 9 9 0.605 9 9 0.666 9 9 0.931 9 9 0.436 group 2 vs 4 nn p 9 9 <0.001 9 9 0.006 9 9 0.113 9 9 0.546 9 9 0.931 9 9 0.605 9 9 0.387 9 9 0.730 group 3 vs 7 nn p 9 9 0.008 9 9 <0.001 9 9 <0.001 9 9 <0.001 9 9 0.004 9 9 0.161 9 9 <0.001 9 9 0.387
group 1 vs 3 nn p 9 9 0.297 9 9 0.077 9 9 0.931 9 9 1.000 9 9 0.063 9 9 1.000 9 9 0.730 9 9 1.000 group 2 vs 5 nn p 9 9 0.003 9 9 0.297 9 9 0.222 9 9 0.666 9 9 0.019 9 9 0.666 9 9 0.094 9 9 0.258 group 4 vs 5 nn p 9 9 0.489 9 9 0.031 9 9 0.031 9 9 0.340 9 9 0.031 9 9 1.000 9 9 0.161 9 9 0.546
Bile acid excretion, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
nn p nn p nn
p
9 9 0.003 9 9 0.019 9 9 <0.001
9 9 0.190 9 9 0.863 9 9 0.258
9 9 0.340 9 9 0.796 9 9 0.258
9 9 0.190 9 9 0.931 9 9 0.002
9 9 0.666 9 9 0.019 9 9 0.340
9 9 0.258 9 9 0.190 9 9 0.003
9 9 1.000 9 9 0.258 9 9 0.297
9 9 0.387 9 9 0.040 9 9 0.094
group 2 vs 6 group 2 vs 7
group 3 vs 4
nn p nn p nn
p
9 9 <0.001 9 9 0.001 9 9 0.002
9 9 0.024 9 9 <0.001 9 9 0.004
9 9 0.040 9 9 <0.001 9 9 0.094
9 9 0.001 9 9 <0.001 9 9 0.161
9 9 0.730 9 9 0.002 9 9 0.094
9 9 0.077 9 9 0.546 9 9 0.387
9 9 0.063 9 9 <0.001 9 9 0.863
9 9 0.258 9 9 0.546 9 9 0.222
group 4 vs 6 group 4 vs 7
group 5 vs 6
nn p nn p nn
p
9 9 0.002 9 9 0.489 9 9 <0.001
9 9 1.000 9 9 0.003 9 9 0.063
9 9 0.003 9 9 <0.001 9 9 0.730
9 9 0.077 9 9 0.011 9 9 0.014
9 9 0.666 9 9 0.003 9 9 0.077
9 9 0.040 9 9 0.796 9 9 0.050
9 9 0.094 9 9 <0.001 9 9 0.931
9 9 0.931 9 9 0.605 9 9 0.931
group 1 vs 7 nn p 9 9 0.019 9 9 0.002 9 9 0.014 9 9 0.001 9 9 0.077 9 9 0.136 9 9 0.001 9 9 0.387 group 3 vs 5 nn p 9 9 0.008 9 9 0.094 9 9 0.340 9 9 0.796 9 9 0.387 9 9 0.340 9 9 0.258 9 9 0.031 group 5 vs 7 nn p 9 9 0.863 9 9 <0.001 9 9 <0.001 9 9 0.002 9 9 <0.001 9 9 0.666 9 9 <0.001 9 9 0.863
group 2 vs 3 nn p 9 9 0.387 9 9 0.258 9 9 1.000 9 9 0.222 9 9 0.040 9 9 0.605 9 9 0.436 9 9 0.340 group 3 vs 6 nn p 9 9 <0.001 9 9 0.008 9 9 0.063 9 9 0.001 9 9 0.161 9 9 0.001 9 9 0.094 9 9 0.050 group 6 vs 7 nn p 9 9 <0.001 9 9 0.002 9 9 0.011 9 9 0.340 9 9 0.002 9 9 0.050 9 9 <0.001 9 9 0.546
p<0.05
Page A82 of A172
Appendix to TNO-report of 3M-02
Final version
E3. Fecal sterol measurements
1:1-1
weight feces
6.2
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 284344
0.98
278657
85.2
60.3
9718
25.13
cholestanol 9225
0.91
8395
2.6
1.8
293
0.75
lathosterol 10102
1
10102
3.1
2.2
352
0.91
campesterol 4671
1.56
7287
2.2
1.6
254
0.63
stigmasterol 1682
1.49
2506
0.8
0.5
87
0.21
b-sitosterol 13713
1.47
20158
6.2
4.4
703
1.70
b-sitostanol
1
0
0.0
0.0
0
0.00
total
323737
327105
100
71
11407
29.3
cholestane 111178
1.04
1:1-2
weight feces
6.5
mg
115625 115625
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 313994
0.98
307714
86.6
76.7
11803
30.52
cholestanol 9355
0.91
8513
2.4
2.1
327
0.84
lathosterol
9015
1
9015
2.5
2.2
346
0.89
campesterol 4796
1.56
7482
2.1
1.9
287
0.72
stigmasterol 1808
1.49
2694
0.8
0.7
103
0.25
b-sitosterol 13562
1.47
19936
5.6
5.0
765
1.84
b-sitostanol
1
0
0.0
0.0
0
0.00
total
352530
355354
100
89
13631
35.1
cholestane 96413
1.04
1:1-3
weight feces
7.1
mg
100270 100270
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 298829
0.98
292852
86.7
73.5
10347
26.76
cholestanol 8558
0.91
7788
2.3
2.0
275
0.71
lathosterol 10265
1
10265
3.0
2.6
363
0.94
campesterol 4356
1.56
6795
2.0
1.7
240
0.60
stigmasterol 1589
1.49
2368
0.7
0.6
84
0.20
b-sitosterol 12044
1.47
17705
5.2
4.4
626
1.51
b-sitostanol
1
0
0.0
0.0
0
0.00
total
335641
337773
100
85
11934
30.7
cholestane 95828
1.04
99661 99661
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A83 of A172
Appendix to TNO-report of 3M-02
Final version
2:1-1
weight feces
7
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 319680
0.98
313286
86.1
87.7
12532
32.41
cholestanol 11436
0.91
10407
2.9
2.9
416
1.07
lathosterol 10467
1
10467
2.9
2.9
419
1.08
campesterol 5023
1.56
7836
2.2
2.2
313
0.78
stigmasterol 1268
1.49
1889
0.5
0.5
76
0.18
b-sitosterol 13626
1.47
20030
5.5
5.6
801
1.93
b-sitostanol
1
0
0.0
0.0
0
0.00
total
361500
363916
100
102
14557
37.5
cholestane 85850
1.04
2:1-2
weight feces 10.4
mg
89284 89284
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 508256
0.98
498091
87.4
131.0
12591
32.56
cholestanol 15011
0.91
13660
2.4
3.6
345
0.89
lathosterol 13004
1
13004
2.3
3.4
329
0.85
campesterol 7404
1.56
11550
2.0
3.0
292
0.73
stigmasterol 2781
1.49
4144
0.7
1.1
105
0.25
b-sitosterol 19988
1.47
29382
5.2
7.7
743
1.79
b-sitostanol
1
0
0.0
0.0
0
0.00
total
566444
569831
100
150
14405
37.1
cholestane 91434
1.04
2:1-3
weight feces
6.8
mg
95091 95091
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 292666
0.98
286813
86.8
89.9
13215
34.17
cholestanol 8651
0.91
7872
2.4
2.5
363
0.93
lathosterol
9600
1
9600
2.9
3.0
442
1.14
campesterol 4193
1.56
6541
2.0
2.0
301
0.75
stigmasterol 1436
1.49
2140
0.6
0.7
99
0.24
b-sitosterol 11876
1.47
17458
5.3
5.5
804
1.94
b-sitostanol
1
0
0.0
0.0
0
0.00
total
328422
330424
100
104
15225
39.2
cholestane 76721
1.04
79790 79790
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A84 of A172
Appendix to TNO-report of 3M-02
Final version
3:1-1
weight feces
6.1
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 440429
0.98
431620
87.6
73.9
12121
31.34
cholestanol 13442
0.91
12232
2.5
2.1
344
0.88
lathosterol 11803
1
11803
2.4
2.0
331
0.86
campesterol 6241
1.56
9736
2.0
1.7
273
0.68
stigmasterol 2114
1.49
3150
0.6
0.5
88
0.21
b-sitosterol 16460
1.47
24196
4.9
4.1
679
1.64
b-sitostanol
1
0
0.0
0.0
0
0.00
total
490489
492738
100
84
13837
35.6
cholestane 140330
1.04
3:1-2
weight feces
5.3
mg
145943 145943
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 237491
0.98
232741
86.7
55.6
10481
27.10
cholestanol 7441
0.91
6771
2.5
1.6
305
0.78
lathosterol
6444
1
6444
2.4
1.5
290
0.75
campesterol 3777
1.56
5892
2.2
1.4
265
0.66
stigmasterol
982
1.49
1463
0.5
0.3
66
0.16
b-sitosterol 10254
1.47
15073
5.6
3.6
679
1.64
b-sitostanol
1
0
0.0
0.0
0
0.00
total
266389
268385
100
64
12086
31.1
cholestane 100714
1.04
3:1-3
weight feces
6.7
mg
104743 104743
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 404372
0.98
396285
88.1
72.6
10838
28.03
cholestanol 11102
0.91
10103
2.2
1.9
276
0.71
lathosterol 11373
1
11373
2.5
2.1
311
0.80
campesterol 5436
1.56
8480
1.9
1.6
232
0.58
stigmasterol 2003
1.49
2984
0.7
0.5
82
0.20
b-sitosterol 14146
1.47
20795
4.6
3.8
569
1.37
b-sitostanol
1
0
0.0
0.0
0
0.00
total
448432
450020
100
82
12307
31.7
cholestane 131190
1.04
136438 136438
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A85 of A172
Appendix to TNO-report of 3M-02
Final version
1:2-4
weight feces
9.1
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 473290
0.98
463824
88.5
123.6
13579
35.12
cholestanol 9709
0.91
8835
1.7
2.4
259
0.67
lathosterol 12754
1
12754
2.4
3.4
373
0.97
campesterol 6031
1.56
9408
1.8
2.5
275
0.69
stigmasterol 2406
1.49
3585
0.7
1.0
105
0.25
b-sitosterol 17298
1.47
25428
4.9
6.8
744
1.80
b-sitostanol
1
0
0.0
0.0
0
0.00
total
521488
523835
100
140
15336
39.5
cholestane 90227
1.04
1:2-5
weight feces
7.2
mg
93836 93836
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 446804
0.98
437868
90.1
114.1
15846
40.98
cholestanol 6124
0.91
5573
1.1
1.5
202
0.52
lathosterol 14209
1
14209
2.9
3.7
514
1.33
campesterol 4560
1.56
7114
1.5
1.9
257
0.64
stigmasterol 1811
1.49
2698
0.6
0.7
98
0.24
b-sitosterol 12767
1.47
18767
3.9
4.9
679
1.64
b-sitostanol
1
0
0.0
0.0
0
0.00
total
486275
486229
100
127
17596
45.3
cholestane 92255
1.04
1:2-6
weight feces
6.2
mg
95945 95945
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 298782
0.98
292806
84.8
65.1
10502
27.16
cholestanol 11596
0.91
10552
3.1
2.3
378
0.97
lathosterol
8473
1
8473
2.5
1.9
304
0.79
campesterol 5240
1.56
8174
2.4
1.8
293
0.73
stigmasterol 1956
1.49
2914
0.8
0.6
105
0.25
b-sitosterol 15108
1.47
22209
6.4
4.9
797
1.92
b-sitostanol
1
0
0.0
0.0
0
0.00
total
341155
345129
100
77
12379
31.8
cholestane 108095
1.04
112419 112419
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A86 of A172
Appendix to TNO-report of 3M-02
Final version
2:2-4
weight feces
8.6
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 481673
0.98
472040
88.7
118.2
13750
35.56
cholestanol 11693
0.91
10641
2.0
2.7
310
0.80
lathosterol 12978
1
12978
2.4
3.3
378
0.98
campesterol 6203
1.56
9677
1.8
2.4
282
0.70
stigmasterol 1175
1.49
1751
0.3
0.4
51
0.12
b-sitosterol 17089
1.47
25121
4.7
6.3
732
1.76
b-sitostanol
1
0
0.0
0.0
0
0.00
total
530811
532206
100
133
15502
39.9
cholestane 95962
1.04
2:2-5
weight feces
9.9
mg
99800 99800
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 434117
0.98
425435
88.8
152.2
15373
39.75
cholestanol 8812
0.91
8019
1.7
2.9
290
0.75
lathosterol 13243
1
13243
2.8
4.7
479
1.24
campesterol 5232
1.56
8162
1.7
2.9
295
0.74
stigmasterol 2068
1.49
3081
0.6
1.1
111
0.27
b-sitosterol 14440
1.47
21227
4.4
7.6
767
1.85
b-sitostanol
1
0
0.0
0.0
0
0.00
total
477912
479167
100
171
17315
44.6
cholestane 67196
1.04
2:2-6
weight feces
6
mg
69884 69884
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 268071
0.98
262710
85.9
72.3
12043
31.14
cholestanol 10152
0.91
9238
3.0
2.5
423
1.09
lathosterol
6784
1
6784
2.2
1.9
311
0.80
campesterol 4476
1.56
6983
2.3
1.9
320
0.80
stigmasterol 1045
1.49
1557
0.5
0.4
71
0.17
b-sitosterol 12736
1.47
18722
6.1
5.1
858
2.07
b-sitostanol
1
0
0.0
0.0
0
0.00
total
303264
305993
100
84
14027
36.1
cholestane 87400
1.04
90896 90896
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A87 of A172
Appendix to TNO-report of 3M-02
Final version
3:2-4
weight feces
5.6
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 269271
0.98
263886
87.6
66.6
11896
30.76
cholestanol 7132
0.91
6490
2.2
1.6
293
0.75
lathosterol
7058
1
7058
2.3
1.8
318
0.82
campesterol 3976
1.56
6203
2.1
1.6
280
0.70
stigmasterol 1401
1.49
2087
0.7
0.5
94
0.23
b-sitosterol 10601
1.47
15583
5.2
3.9
702
1.69
b-sitostanol
1
0
0.0
0.0
0
0.00
total
299439
301307
100
76
13582
35.0
cholestane 95225
1.04
3:2-5
weight feces
6.9
mg
99034 99034
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 398275
0.98
390310
89.5
79.9
11581
29.95
cholestanol 7905
0.91
7194
1.6
1.5
213
0.55
lathosterol 11474
1
11474
2.6
2.3
340
0.88
campesterol 4821
1.56
7521
1.7
1.5
223
0.56
stigmasterol 1507
1.49
2245
0.5
0.5
67
0.16
b-sitosterol 11865
1.47
17442
4.0
3.6
518
1.25
b-sitostanol
1
0
0.0
0.0
0
0.00
total
435847
436185
100
89
12942
33.3
cholestane 117414
1.04
3:2-6
weight feces
7.2
mg
122111 122111
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 336997
0.98
330257
86.2
80.7
11206
28.98
cholestanol 12422
0.91
11304
2.9
2.8
384
0.99
lathosterol
7963
1
7963
2.1
1.9
270
0.70
campesterol 5484
1.56
8555
2.2
2.1
290
0.72
stigmasterol 2009
1.49
2993
0.8
0.7
102
0.25
b-sitosterol 15075
1.47
22160
5.8
5.4
752
1.81
b-sitostanol
1
0
0.0
0.0
0
0.00
total
379950
383233
100
94
13003
33.4
cholestane 98396
1.04
102332 102332
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A88 of A172
Appendix to TNO-report of 3M-02
Final version
1:3-7
weight feces
6
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 240788
0.98
235972
85.5
67.4
11229
29.04
cholestanol 7267
0.91
6613
2.4
1.9
315
0.81
lathosterol
9283
1
9283
3.4
2.7
442
1.14
campesterol 3808
1.56
5940
2.2
1.7
283
0.71
stigmasterol 1488
1.49
2217
0.8
0.6
106
0.26
b-sitosterol 10778
1.47
15844
5.7
4.5
754
1.82
b-sitostanol
1
0
0.0
0.0
0
0.00
total
273412
275869
100
79
13128
33.8
cholestane 84191
1.04
1:3-8
weight feces
7
mg
87559 87559
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 283543
0.98
277872
85.5
71.7
10245
26.49
cholestanol 10969
0.91
9982
3.1
2.6
368
0.95
lathosterol
8285
1
8285
2.5
2.1
305
0.79
campesterol 4663
1.56
7274
2.2
1.9
268
0.67
stigmasterol 1774
1.49
2643
0.8
0.7
97
0.24
b-sitosterol 12953
1.47
19041
5.9
4.9
702
1.69
b-sitostanol
1
0
0.0
0.0
0
0.00
total
322187
325097
100
84
11986
30.8
cholestane 93139
1.04
1:3-9
weight feces 10.8
mg
96865 96865
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 529012
0.98
518432
89.0
153.4
14205
36.73
cholestanol 14038
0.91
12775
2.2
3.8
350
0.90
lathosterol 11702
1
11702
2.0
3.5
321
0.83
campesterol 6647
1.56
10369
1.8
3.1
284
0.71
stigmasterol 1317
1.49
1962
0.3
0.6
54
0.13
b-sitosterol 18688
1.47
27471
4.7
8.1
753
1.82
b-sitostanol
1
0
0.0
0.0
0
0.00
total
581404
582711
100
172
15966
41.1
cholestane 81233
1.04
84482 84482
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A89 of A172
Appendix to TNO-report of 3M-02
Final version
2:3-7
weight feces
8
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 330311
0.98
323705
86.2
95.0
11875
30.71
cholestanol 9369
0.91
8526
2.3
2.5
313
0.80
lathosterol 11675
1
11675
3.1
3.4
428
1.11
campesterol 5157
1.56
8045
2.1
2.4
295
0.74
stigmasterol 1936
1.49
2885
0.8
0.8
106
0.26
b-sitosterol 14114
1.47
20748
5.5
6.1
761
1.84
b-sitostanol
1
0
0.0
0.0
0
0.00
total
372562
375583
100
110
13778
35.4
cholestane 81910
1.04
2:3-8
weight feces
7.3
mg
85186 85186
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 361988
0.98
354748
87.7
89.9
12322
31.86
cholestanol 10955
0.91
9969
2.5
2.5
346
0.89
lathosterol 10492
1
10492
2.6
2.7
364
0.94
campesterol 4873
1.56
7602
1.9
1.9
264
0.66
stigmasterol 1595
1.49
2377
0.6
0.6
83
0.20
b-sitosterol 13274
1.47
19513
4.8
4.9
678
1.63
b-sitostanol
1
0
0.0
0.0
0
0.00
total
403177
404701
100
103
14057
36.2
cholestane 94804
1.04
2:3-9
weight feces
7.7
mg
98596 98596
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 409014
0.98
400834
88.8
109.4
14211
36.75
cholestanol 11289
0.91
10273
2.3
2.8
364
0.94
lathosterol
9106
1
9106
2.0
2.5
323
0.83
campesterol 5174
1.56
8071
1.8
2.2
286
0.71
stigmasterol 1042
1.49
1553
0.3
0.4
55
0.13
b-sitosterol 14716
1.47
21633
4.8
5.9
767
1.85
b-sitostanol
1
0
0.0
0.0
0
0.00
total
450341
451469
100
123
16006
41.2
cholestane 88054
1.04
91576 91576
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A90 of A172
Appendix to TNO-report of 3M-02
Final version
3:3-7
weight feces
8.2
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 428635
0.98
420062
87.0
95.1
11598
29.99
cholestanol 12166
0.91
11071
2.3
2.5
306
0.79
lathosterol 13406
1
13406
2.8
3.0
370
0.96
campesterol 6496
1.56
10134
2.1
2.3
280
0.70
stigmasterol 2441
1.49
3637
0.8
0.8
100
0.24
b-sitosterol 16860
1.47
24784
5.1
5.6
684
1.65
b-sitostanol
1
0
0.0
0.0
0
0.00
total
480004
483094
100
109
13338
34.3
cholestane 106174
1.04
3:3-8
weight feces
7.6
mg
110421 110421
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 370433
0.98
363024
86.3
74.7
9828
25.41
cholestanol 13556
0.91
12336
2.9
2.5
334
0.86
lathosterol
9317
1
9317
2.2
1.9
252
0.65
campesterol 6050
1.56
9438
2.2
1.9
256
0.64
stigmasterol 2043
1.49
3044
0.7
0.6
82
0.20
b-sitosterol 15825
1.47
23263
5.5
4.8
630
1.52
b-sitostanol
1
0
0.0
0.0
0
0.00
total
417224
420422
100
87
11382
29.3
cholestane 116833
1.04
3:3-9
weight feces
6
mg
121506 121506
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 327522
0.98
320972
88.4
71.3
11877
30.71
cholestanol 9565
0.91
8704
2.4
1.9
322
0.83
lathosterol
7411
1
7411
2.0
1.6
274
0.71
campesterol 4458
1.56
6954
1.9
1.5
257
0.64
stigmasterol 1015
1.49
1512
0.4
0.3
56
0.14
b-sitosterol 11869
1.47
17447
4.8
3.9
646
1.56
b-sitostanol
1
0
0.0
0.0
0
0.00
total
361840
363001
100
81
13432
34.6
cholestane 108272
1.04
112603 112603
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A91 of A172
Appendix to TNO-report of 3M-02
Final version
1:4-10
weight feces
5.9
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 362101
0.98
354859
88.7
81.6
13829
35.76
cholestanol 9381
0.91
8537
2.1
2.0
333
0.86
lathosterol
7899
1
7899
2.0
1.8
308
0.80
campesterol 4622
1.56
7210
1.8
1.7
281
0.70
stigmasterol 1882
1.49
2804
0.7
0.6
109
0.26
b-sitosterol 12850
1.47
18890
4.7
4.3
736
1.78
b-sitostanol
1
0
0.0
0.0
0
0.00
total
398735
400199
100
92
15595
40.2
cholestane 104552
1.04
1:4-11
weight feces
8
mg
108734 108734
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 489702
0.98
479908
90.0
107.0
13380
34.60
cholestanol 15062
0.91
13706
2.6
3.1
382
0.98
lathosterol 10888
1
10888
2.0
2.4
304
0.78
campesterol 4869
1.56
7596
1.4
1.7
212
0.53
stigmasterol
748
1.49
1115
0.2
0.2
31
0.08
b-sitosterol 13552
1.47
19921
3.7
4.4
555
1.34
b-sitostanol
1
0
0.0
0.0
0
0.00
total
534821
533134
100
119
14864
38.3
cholestane 107776
1.04
1:4-12
weight feces
6.1
mg
112087 112087
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 459088
0.98
449906
89.5
96.7
15857
41.01
cholestanol 15039
0.91
13685
2.7
2.9
482
1.24
lathosterol 11182
1
11182
2.2
2.4
394
1.02
campesterol 4797
1.56
7483
1.5
1.6
264
0.66
stigmasterol
686
1.49
1022
0.2
0.2
36
0.09
b-sitosterol 13085
1.47
19235
3.8
4.1
678
1.63
b-sitostanol
1
0
0.0
0.0
0
0.00
total
503877
502514
100
108
17711
45.6
cholestane 111810
1.04
116282 116282
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A92 of A172
Appendix to TNO-report of 3M-02
Final version
2:4-10
weight feces
7.7
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 521217
0.98
510793
90.7
142.2
18473
47.77
cholestanol 14915
0.91
13573
2.4
3.8
491
1.26
lathosterol 11055
1
11055
2.0
3.1
400
1.03
campesterol 4779
1.56
7455
1.3
2.1
270
0.67
stigmasterol
743
1.49
1107
0.2
0.3
40
0.10
b-sitosterol 13109
1.47
19270
3.4
5.4
697
1.68
b-sitostanol
1
0
0.0
0.0
0
0.00
total
565818
563253
100
157
20370
52.5
cholestane 86322
1.04
2:4-11
weight feces
6.4
mg
89775 89775
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 497491
0.98
487541
90.8
118.8
18567
48.01
cholestanol 13351
0.91
12149
2.3
3.0
463
1.19
lathosterol 10137
1
10137
1.9
2.5
386
1.00
campesterol 4786
1.56
7466
1.4
1.8
284
0.71
stigmasterol
727
1.49
1083
0.2
0.3
41
0.10
b-sitosterol 12456
1.47
18310
3.4
4.5
697
1.68
b-sitostanol
1
0
0.0
0.0
0
0.00
total
538948
536687
100
131
20439
52.7
cholestane 98627
1.04
2:4-12
weight feces
8.9
mg
102572 102572
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 572090
0.98
560648
90.6
171.6
19286
49.87
cholestanol 15045
0.91
13691
2.2
4.2
471
1.21
lathosterol 12013
1
12013
1.9
3.7
413
1.07
campesterol 5734
1.56
8945
1.4
2.7
308
0.77
stigmasterol
676
1.49
1007
0.2
0.3
35
0.08
b-sitosterol 15286
1.47
22470
3.6
6.9
773
1.86
b-sitostanol
1
0
0.0
0.0
0
0.00
total
620844
618775
100
189
21285
54.9
cholestane 78519
1.04
81660 81660
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A93 of A172
Appendix to TNO-report of 3M-02
Final version
3:4-10
weight feces
6.8
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 434209
0.98
425525
90.4
89.6
13179
34.08
cholestanol 12431
0.91
11312
2.4
2.4
350
0.90
lathosterol
9196
1
9196
2.0
1.9
285
0.74
campesterol 4448
1.56
6939
1.5
1.5
215
0.54
stigmasterol
651
1.49
970
0.2
0.2
30
0.07
b-sitosterol 11421
1.47
16789
3.6
3.5
520
1.25
b-sitostanol
1
0
0.0
0.0
0
0.00
total
472356
470731
100
99
14579
37.6
cholestane 114142
1.04
3:4-11
weight feces
6.8
mg
118708 118708
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 690765
0.98
676950
91.4
122.7
18042
46.66
cholestanol 15497
0.91
14102
1.9
2.6
376
0.97
lathosterol 14645
1
14645
2.0
2.7
390
1.01
campesterol 6317
1.56
9855
1.3
1.8
263
0.66
stigmasterol
805
1.49
1199
0.2
0.2
32
0.08
b-sitosterol 16234
1.47
23864
3.2
4.3
636
1.53
b-sitostanol
1
0
0.0
0.0
0
0.00
total
744263
740615
100
134
19739
50.9
cholestane 132638
1.04
3:4-12
weight feces
7.7
mg
137944 137944
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 657017
0.98
643877
91.0
121.8
15814
40.89
cholestanol 16315
0.91
14847
2.1
2.8
365
0.94
lathosterol 13781
1
13781
1.9
2.6
338
0.88
campesterol 6401
1.56
9986
1.4
1.9
245
0.61
stigmasterol
812
1.49
1210
0.2
0.2
30
0.07
b-sitosterol 16291
1.47
23948
3.4
4.5
588
1.42
b-sitostanol
1
0
0.0
0.0
0
0.00
total
710617
707648
100
134
17380
44.8
cholestane 127109
1.04
132193 132193
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A94 of A172
Appendix to TNO-report of 3M-02
Final version
1:5-13
weight feces
7.8
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 482166
0.98
472523
89.1
129.2
16561
42.83
cholestanol 15148
0.91
13785
2.6
3.8
483
1.24
lathosterol 13001
1
13001
2.5
3.6
456
1.18
campesterol 5131
1.56
8004
1.5
2.2
281
0.70
stigmasterol 1060
1.49
1579
0.3
0.4
55
0.13
b-sitosterol 14512
1.47
21333
4.0
5.8
748
1.80
b-sitostanol
1
0
0.0
0.0
0
0.00
total
531018
530225
100
145
18583
47.9
cholestane 87934
1.04
1:5-14
weight feces 13.4
mg
91451 91451
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 634279
0.98
621593
89.9
187.2
13972
36.13
cholestanol 12599
0.91
11465
1.7
3.5
258
0.66
lathosterol 16167
1
16167
2.3
4.9
363
0.94
campesterol 6696
1.56
10446
1.5
3.1
235
0.59
stigmasterol 2927
1.49
4361
0.6
1.3
98
0.24
b-sitosterol 18799
1.47
27635
4.0
8.3
621
1.50
b-sitostanol
1
0
0.0
0.0
0
0.00
total
691467
691667
100
208
15547
40.1
cholestane 79811
1.04
1:5-15
weight feces
6.2
mg
83003 83003
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 255399
0.98
250291
85.5
66.1
10669
27.59
cholestanol 13187
0.91
12000
4.1
3.2
512
1.32
lathosterol
6307
1
6307
2.2
1.7
269
0.70
campesterol 3843
1.56
5995
2.0
1.6
256
0.64
stigmasterol
729
1.49
1086
0.4
0.3
46
0.11
b-sitosterol 11657
1.47
17136
5.9
4.5
730
1.76
b-sitostanol
1
0
0.0
0.0
0
0.00
total
291122
292815
100
77
12481
32.1
cholestane 90959
1.04
94597 94597
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A95 of A172
Appendix to TNO-report of 3M-02
Final version
2:5-13
weight feces
7.9
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 455921
0.98
446803
89.0
124.6
15774
40.79
cholestanol 13655
0.91
12426
2.5
3.5
439
1.13
lathosterol 12928
1
12928
2.6
3.6
456
1.18
campesterol 5203
1.56
8117
1.6
2.3
287
0.72
stigmasterol
898
1.49
1338
0.3
0.4
47
0.11
b-sitosterol 13941
1.47
20493
4.1
5.7
723
1.74
b-sitostanol
1
0
0.0
0.0
0
0.00
total
502546
502105
100
140
17726
45.7
cholestane 86190
1.04
2:5-14
weight feces
6.9
mg
89638 89638
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 302926
0.98
296867
90.2
101.4
14697
38.01
cholestanol 6271
0.91
5707
1.7
1.9
283
0.73
lathosterol
8314
1
8314
2.5
2.8
412
1.06
campesterol 3149
1.56
4912
1.5
1.7
243
0.61
stigmasterol
541
1.49
806
0.2
0.3
40
0.10
b-sitosterol
8475
1.47
12458
3.8
4.3
617
1.49
b-sitostanol
1
0
0.0
0.0
0
0.00
total
329676
329065
100
112
16291
42.0
cholestane 70369
1.04
2:5-15
weight feces
4.8
mg
73184 73184
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 210645
0.98
206432
83.6
52.2
10870
28.11
cholestanol 13493
0.91
12279
5.0
3.1
647
1.66
lathosterol
5461
1
5461
2.2
1.4
288
0.74
campesterol 3832
1.56
5978
2.4
1.5
315
0.79
stigmasterol
733
1.49
1092
0.4
0.3
58
0.14
b-sitosterol 10573
1.47
15542
6.3
3.9
818
1.97
b-sitostanol
1
0
0.0
0.0
0
0.00
total
244737
246784
100
62
12995
33.4
cholestane 95106
1.04
98910 98910
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A96 of A172
Appendix to TNO-report of 3M-02
Final version
3:5-13
weight feces 11.8
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 760655
0.98
745442
88.7
146.6
12425
32.13
cholestanol 21051
0.91
19156
2.3
3.8
319
0.82
lathosterol 19855
1
19855
2.4
3.9
331
0.86
campesterol 9056
1.56
14127
1.7
2.8
235
0.59
stigmasterol 3585
1.49
5342
0.6
1.1
89
0.22
b-sitosterol 24867
1.47
36554
4.3
7.2
609
1.47
b-sitostanol
1
0
0.0
0.0
0
0.00
total
839069
840477
100
165
14009
36.1
cholestane 122218
1.04
3:5-14
weight feces
7.2
mg
127107 127107
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 475539
0.98
466028
90.3
88.3
12257
31.70
cholestanol 8894
0.91
8094
1.6
1.5
213
0.55
lathosterol 11319
1
11319
2.2
2.1
298
0.77
campesterol 4936
1.56
7700
1.5
1.5
203
0.51
stigmasterol 2133
1.49
3178
0.6
0.6
84
0.20
b-sitosterol 13417
1.47
19723
3.8
3.7
519
1.25
b-sitostanol
1
0
0.0
0.0
0
0.00
total
516238
516042
100
98
13573
35.0
cholestane 126938
1.04
3:5-15
weight feces
7.1
mg
132016 132016
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 369324
0.98
361938
85.4
62.2
8763
22.66
cholestanol 21096
0.91
19197
4.5
3.3
465
1.20
lathosterol
8285
1
8285
2.0
1.4
201
0.52
campesterol 6116
1.56
9541
2.3
1.6
231
0.58
stigmasterol 1212
1.49
1806
0.4
0.3
44
0.11
b-sitosterol 15819
1.47
23254
5.5
4.0
563
1.36
b-sitostanol
1
0
0.0
0.0
0
0.00
total
421852
424021
100
73
10267
26.4
cholestane 139834
1.04
145427 145427
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A97 of A172
Appendix to TNO-report of 3M-02
Final version
1:6-16
weight feces
5.5
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 316760
0.98
310425
88.4
75.3
13682
35.38
cholestanol 10679
0.91
9718
2.8
2.4
428
1.10
lathosterol
7883
1
7883
2.2
1.9
347
0.90
campesterol 3863
1.56
6026
1.7
1.5
266
0.66
stigmasterol
573
1.49
854
0.2
0.2
38
0.09
b-sitosterol 10951
1.47
16098
4.6
3.9
710
1.71
b-sitostanol
1
0
0.0
0.0
0
0.00
total
350709
351004
100
85
15471
39.8
cholestane 99161
1.04
1:6-17
weight feces
5.1
mg
103127 103127
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 283717
0.98
278043
86.7
61.2
12006
31.05
cholestanol 12638
0.91
11501
3.6
2.5
497
1.28
lathosterol
7544
1
7544
2.4
1.7
326
0.84
campesterol 4033
1.56
6291
2.0
1.4
272
0.68
stigmasterol
593
1.49
884
0.3
0.2
38
0.09
b-sitosterol 11162
1.47
16408
5.1
3.6
709
1.71
b-sitostanol
1
0
0.0
0.0
0
0.00
total
319687
320670
100
71
13847
35.6
cholestane 109156
1.04
1:6-18
weight feces
9.3
mg
113522 113522
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 432750
0.98
424095
87.8
121.3
13048
33.74
cholestanol 15694
0.91
14282
3.0
4.1
439
1.13
lathosterol 11216
1
11216
2.3
3.2
345
0.89
campesterol 5531
1.56
8628
1.8
2.5
265
0.66
stigmasterol
828
1.49
1234
0.3
0.4
38
0.09
b-sitosterol 15866
1.47
23323
4.8
6.7
718
1.73
b-sitostanol
1
0
0.0
0.0
0
0.00
total
481885
482778
100
138
14853
38.2
cholestane 84015
1.04
87376 87376
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A98 of A172
Appendix to TNO-report of 3M-02
Final version
2:6-16
weight feces
5.2
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 306320
0.98
300194
89.1
71.3
13705
35.44
cholestanol 10414
0.91
9477
2.8
2.2
433
1.11
lathosterol
7025
1
7025
2.1
1.7
321
0.83
campesterol 3400
1.56
5304
1.6
1.3
242
0.60
stigmasterol
583
1.49
869
0.3
0.2
40
0.10
b-sitosterol
9578
1.47
14080
4.2
3.3
643
1.55
b-sitostanol
1
0
0.0
0.0
0
0.00
total
337320
336948
100
80
15383
39.6
cholestane 101256
1.04
2:6-17
weight feces
4.6
mg
105306 105306
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 264813
0.98
259517
86.0
58.0
12612
32.61
cholestanol 13978
0.91
12720
4.2
2.8
618
1.59
lathosterol
6351
1
6351
2.1
1.4
309
0.80
campesterol 4076
1.56
6359
2.1
1.4
309
0.77
stigmasterol
392
1.49
584
0.2
0.1
28
0.07
b-sitosterol 11081
1.47
16289
5.4
3.6
792
1.91
b-sitostanol
1
0
0.0
0.0
0
0.00
total
300691
301819
100
67
14667
37.8
cholestane 107534
1.04
2:6-18
weight feces
7.1
mg
111835 111835
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 415098
0.98
406796
88.0
89.1
12553
32.46
cholestanol 15248
0.91
13876
3.0
3.0
428
1.10
lathosterol 11257
1
11257
2.4
2.5
347
0.90
campesterol 5140
1.56
8018
1.7
1.8
247
0.62
stigmasterol
790
1.49
1177
0.3
0.3
36
0.09
b-sitosterol 14501
1.47
21316
4.6
4.7
658
1.59
b-sitostanol
1
0
0.0
0.0
0
0.00
total
462034
462441
100
101
14270
36.8
cholestane 109718
1.04
114107 114107
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A99 of A172
Appendix to TNO-report of 3M-02
Final version
3:6-16
weight feces
9.4
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 528030
0.98
517469
90.2
120.9
12861
33.26
cholestanol 15892
0.91
14462
2.5
3.4
359
0.92
lathosterol 10925
1
10925
1.9
2.6
272
0.70
campesterol 5503
1.56
8585
1.5
2.0
213
0.53
stigmasterol
761
1.49
1134
0.2
0.3
28
0.07
b-sitosterol 14410
1.47
21183
3.7
4.9
526
1.27
b-sitostanol
1
0
0.0
0.0
0
0.00
total
575521
573757
100
134
14260
36.8
cholestane 102895
1.04
3:6-17
weight feces
5.2
mg
107011 107011
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 325132
0.98
318629
87.3
55.5
10671
27.59
cholestanol 15757
0.91
14339
3.9
2.5
480
1.24
lathosterol
7236
1
7236
2.0
1.3
242
0.63
campesterol 4495
1.56
7012
1.9
1.2
235
0.59
stigmasterol
611
1.49
910
0.2
0.2
30
0.07
b-sitosterol 11479
1.47
16874
4.6
2.9
565
1.36
b-sitostanol
1
0
0.0
0.0
0
0.00
total
364710
365001
100
64
12224
31.5
cholestane 138038
1.04
3:6-18
weight feces
6.9
mg
143560 143560
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 716301
0.98
701975
88.7
96.4
13976
36.14
cholestanol 24577
0.91
22365
2.8
3.1
445
1.15
lathosterol 18733
1
18733
2.4
2.6
373
0.96
campesterol 8508
1.56
13272
1.7
1.8
264
0.66
stigmasterol 1102
1.49
1642
0.2
0.2
33
0.08
b-sitosterol 22755
1.47
33450
4.2
4.6
666
1.61
b-sitostanol
1
0
0.0
0.0
0
0.00
total
791976
791437
100
109
15758
40.6
cholestane 174978
1.04
181977 181977
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A100 of A172
Appendix to TNO-report of 3M-02
Final version
1:7-19
weight feces
7.5
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 449806
0.98
440810
86.5
98.4
13115
33.92
cholestanol 25289
0.91
23013
4.5
5.1
685
1.76
lathosterol
7667
1
7667
1.5
1.7
228
0.59
campesterol 6477
1.56
10104
2.0
2.3
301
0.75
stigmasterol
917
1.49
1366
0.3
0.3
41
0.10
b-sitosterol 18151
1.47
26682
5.2
6.0
794
1.91
b-sitostanol
1
0
0.0
0.0
0
0.00
total
508307
509642
100
114
15163
39.0
cholestane 107726
1.04
1:7-20
weight feces 10.8
mg
112035 112035
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 497481
0.98
487531
85.5
119.2
11041
28.55
cholestanol 30777
0.91
28007
4.9
6.9
634
1.63
lathosterol
9362
1
9362
1.6
2.3
212
0.55
campesterol 7714
1.56
12034
2.1
2.9
273
0.68
stigmasterol 1041
1.49
1551
0.3
0.4
35
0.09
b-sitosterol 21441
1.47
31518
5.5
7.7
714
1.72
b-sitostanol
1
0
0.0
0.0
0
0.00
total
567816
570004
100
139
12909
33.2
cholestane 98281
1.04
1:7-21
weight feces
8.5
mg
102212 102212
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 328489
0.98
321919
86.3
100.2
11792
30.49
cholestanol 18232
0.91
16591
4.4
5.2
608
1.56
lathosterol
5881
1
5881
1.6
1.8
215
0.56
campesterol 5028
1.56
7844
2.1
2.4
287
0.72
stigmasterol
654
1.49
974
0.3
0.3
36
0.09
b-sitosterol 13624
1.47
20027
5.4
6.2
734
1.77
b-sitostanol
1
0
0.0
0.0
0
0.00
total
371908
373237
100
116
13671
35.2
cholestane 77207
1.04
80295 80295
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A101 of A172
Appendix to TNO-report of 3M-02
Final version
2:7-19
weight feces
7.3
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 393028
0.98
385167
87.2
87.9
12046
31.15
cholestanol 19805
0.91
18023
4.1
4.1
564
1.45
lathosterol
6961
1
6961
1.6
1.6
218
0.56
campesterol 5235
1.56
8167
1.8
1.9
255
0.64
stigmasterol 2749
1.49
4096
0.9
0.9
128
0.31
b-sitosterol 13255
1.47
19485
4.4
4.4
609
1.47
b-sitostanol
1
0
0.0
0.0
0
0.00
total
441033
441898
100
101
13820
35.6
cholestane 105292
1.04
2:7-20
weight feces
7
mg
109504 109504
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 363884
0.98
356606
86.1
91.7
13105
33.89
cholestanol 22163
0.91
20168
4.9
5.2
741
1.91
lathosterol
6943
1
6943
1.7
1.8
255
0.66
campesterol 5485
1.56
8557
2.1
2.2
314
0.78
stigmasterol
752
1.49
1120
0.3
0.3
41
0.10
b-sitosterol 14149
1.47
20799
5.0
5.4
764
1.84
b-sitostanol
1
0
0.0
0.0
0
0.00
total
413376
414194
100
107
15222
39.2
cholestane 93444
1.04
2:7-21
weight feces
6.9
mg
97182 97182
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 399924
0.98
391926
87.3
88.6
12845
33.22
cholestanol 19224
0.91
17494
3.9
4.0
573
1.48
lathosterol
7234
1
7234
1.6
1.6
237
0.61
campesterol 5395
1.56
8416
1.9
1.9
276
0.69
stigmasterol 2024
1.49
3016
0.7
0.7
99
0.24
b-sitosterol 14143
1.47
20790
4.6
4.7
681
1.64
b-sitostanol
1
0
0.0
0.0
0
0.00
total
447944
448876
100
102
14711
37.9
cholestane 106300
1.04
110552 110552
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A102 of A172
Appendix to TNO-report of 3M-02
Final version
3:7-19
weight feces
10
mg
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 582689
0.98
571035
88.5
114.6
11457
29.63
cholestanol 23901
0.91
21750
3.4
4.4
436
1.12
lathosterol 10390
1
10390
1.6
2.1
208
0.54
campesterol 7529
1.56
11745
1.8
2.4
236
0.59
stigmasterol
942
1.49
1404
0.2
0.3
28
0.07
b-sitosterol 19466
1.47
28615
4.4
5.7
574
1.38
b-sitostanol
1
0
0.0
0.0
0
0.00
total
644917
644939
100
129
12940
33.3
cholestane 119811
1.04
3:7-20
weight feces
4.9
mg
124603 124603
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 282329
0.98
276682
87.2
56.9
11609
30.02
cholestanol 16221
0.91
14761
4.7
3.0
619
1.59
lathosterol
5485
1
5485
1.7
1.1
230
0.60
campesterol 3642
1.56
5682
1.8
1.2
238
0.59
stigmasterol
437
1.49
651
0.2
0.1
27
0.07
b-sitosterol
9568
1.47
14065
4.4
2.9
590
1.42
b-sitostanol
1
0
0.0
0.0
0
0.00
total
317682
317326
100
65
13314
34.3
cholestane 116924
1.04
3:7-21
weight feces
9.5
mg
121601 121601
area response area corrected
%
g sterol ng sterol/ nmol sterol/
factor for response factor
mg feces mg feces
coprostanol
1
0
0.0
0.0
0
0.00
coprostanon
1
0
0.0
0.0
0
0.00
cholesterol 486322
0.98
476596
88.6
110.7
11653
30.13
cholestanol 21163
0.91
19258
3.6
4.5
471
1.21
lathosterol
7823
1
7823
1.5
1.8
191
0.49
campesterol 6293
1.56
9817
1.8
2.3
240
0.60
stigmasterol
622
1.49
927
0.2
0.2
23
0.05
b-sitosterol 16081
1.47
23639
4.4
5.5
578
1.39
b-sitostanol
1
0
0.0
0.0
0
0.00
total
538304
538060
100
125
13155
33.9
cholestane 103492
1.04
107632 107632
coprostanol coprostanon cholesterol cholestanol lathosterol campesterol stigmasterol b-sitosterol b-sitostanol
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
388.7 386.7 386.7 388.7 386.7 400.7 412.7 414.7 416.7
04-12-2006
Page A103 of A172
Page A104 of A172
group
cage#
GC
neutral sterols (mol/g feces)
neutral sterols total feces average weight days
sample# coprostanol coprostanon cholesterol cholestanol lathosterol total (mol/g feces)
(g)
(g)
1
1,2
1:1-1
0.00
0.00
25.13
0.75
0.91
26.79
3.075
123.6
2
1
3,4
1:1-2
0.00
0.00
30.52
0.84
0.89
32.26
2.4897
87.25
2
1
5
1:1-3
0.00
0.00
26.76
0.71
0.94
28.40
2.2946
88.55
2
1
1,2
2:1-1
0.00
0.00
32.41
1.07
1.08
34.56
4.692
122.45
3
1
3,4
2:1-2
0.00
0.00
32.56
0.89
0.85
34.30
3.7789
86.55
3
1
5
2:1-3
0.00
0.00
34.17
0.93
1.14
36.25
3.4106
88.2
3
1
1,2
3:1-1
0.00
0.00
31.34
0.88
0.86
33.08
3.5754
121.65
2
1
3,4
3:1-2
0.00
0.00
27.10
0.78
0.75
28.64
2.8781
86.55
2
1
5
3:1-3
0.00
0.00
28.03
0.71
0.80
29.54
2.7589
88.05
2
AVERAGE
SD
2
6
1:2-4
0.00
0.00
35.12
0.67
0.97
36.75
2.7619
94.55
2
2
7,8
1:2-5
0.00
0.00
40.98
0.52
1.33
42.83
4.0581
126.6
2
2
9,10 1:2-6
0.00
0.00
27.16
0.97
0.79
28.92
2.0935
82.2
2
2
6
2:2-4
0.00
0.00
35.56
0.80
0.98
37.33
4.075
94.25
3
2
7,8
2:2-5
0.00
0.00
39.75
0.75
1.24
41.74
6.371
126.45
3
2
9,10 2:2-6
0.00
0.00
31.14
1.09
0.80
33.04
3.4074
82.1
3
2
6
3:2-4
0.00
0.00
30.76
0.75
0.82
32.34
2.9279
94
2
2
7,8
3:2-5
0.00
0.00
29.95
0.55
0.88
31.38
4.3985
126.6
2
2
9,10 3:2-6
0.00
0.00
28.98
0.99
0.70
30.66
2.4575
81.85
2
AVERAGE
SD
3
11,12,13 1:3-7
0.00
0.00
29.04
0.81
1.14
30.99
3.4597
118
2
3
15
1:3-8
0.00
0.00
26.49
0.95
0.79
28.23
2.2994
83.55
2
3
14,16,17 1:3-9
0.00
0.00
36.73
0.90
0.83
38.46
2.3454
82.8
2
3
11,12,13 2:3-7
0.00
0.00
30.71
0.80
1.11
32.62
5.8147
118.75
3
3
15
2:3-8
0.00
0.00
31.86
0.89
0.94
33.70
3.7718
83.5
3
3
14,16,17 2:3-9
0.00
0.00
36.75
0.94
0.83
38.52
3.7727
83.05
3
3
11,12,13 3:3-7
0.00
0.00
29.99
0.79
0.96
31.74
4.025
119.6
2
3
15
3:3-8
0.00
0.00
25.41
0.86
0.65
26.93
2.5265
84.2
2
3
14,16,17 3:3-9
0.00
0.00
30.71
0.83
0.71
32.25
2.6905
83.45
2
AVERAGE
SD
4
18
1:4-10
0.00
0.00
35.76
0.86
0.80
37.41
1.689
59.5
2
4
19
1:4-11
0.00
0.00
34.60
0.98
0.78
36.37
3.1432
116.4
2
4
20,21 1:4-12
0.00
0.00
41.01
1.24
1.02
43.27
3.2545
112.2
2
4
18
2:4-10
0.00
0.00
47.77
1.26
1.03
50.07
2.7133
58.85
3
4
19
2:4-11
0.00
0.00
48.01
1.19
1.00
50.20
4.6407
116.55
3
4
20,21 2:4-12
0.00
0.00
49.87
1.21
1.07
52.15
5.2075
111.85
3
4
18
3:4-10
0.00
0.00
34.08
0.90
0.74
35.72
2.041
58.7
2
4
19
3:4-11
0.00
0.00
46.66
0.97
1.01
48.63
3.4464
117.45
2
4
20,21 3:4-12
0.00
0.00
40.89
0.94
0.88
42.71
3.9549
112.9
2
AVERAGE
SD
5
22
1:5-13
0.00
0.00
42.83
1.24
1.18
45.25
3.195
110.7
2
5
24,25 1:5-14
0.00
0.00
36.13
0.66
0.94
37.73
2.9408
88.35
2
5
23,26,27 1:5-15
0.00
0.00
27.59
1.32
0.70
29.60
2.6399
84.6
2
5
22
2:5-13
0.00
0.00
40.79
1.13
1.18
43.10
4.9712
111.6
3
5
24,25 2:5-14
0.00
0.00
38.01
0.73
1.06
39.80
4.4833
88.5
3
5
23,26,27 2:5-15
0.00
0.00
28.11
1.66
0.74
30.52
3.767
85.55
3
5
22
3:5-13
0.00
0.00
32.13
0.82
0.86
33.81
3.6113
112.85
2
5
24,25 3:5-14
0.00
0.00
31.70
0.55
0.77
33.01
3.3522
88.9
2
5
23,26,27 3:5-15
0.00
0.00
22.66
1.20
0.52
24.38
2.7034
85.5
2
AVERAGE
SD
6
28,29 1:6-16
0.00
0.00
35.38
1.10
0.90
37.38
2.5036
87.1
2
6
31
1:6-17
0.00
0.00
31.05
1.28
0.84
33.17
2.7083
112.8
2
6
30,32 1:6-18
0.00
0.00
33.74
1.13
0.89
35.76
2.3554
85.4
2
6
28,29 2:6-16
0.00
0.00
35.44
1.11
0.83
37.38
3.9684
87.15
3
6
31
2:6-17
0.00
0.00
32.61
1.59
0.80
35.00
3.9678
112.45
3
6
30,32 2:6-18
0.00
0.00
32.46
1.10
0.90
34.46
3.5666
84.55
3
6
28,29 3:6-16
0.00
0.00
33.26
0.92
0.70
34.88
2.9076
87.65
2
6
31
3:6-17
0.00
0.00
27.59
1.24
0.63
29.46
3.2449
111.95
2
6
30,32 3:6-18
0.00
0.00
36.14
1.15
0.96
38.25
2.7643
84.65
2
AVERAGE
SD
7
33,34,35 1:7-19
0.00
0.00
33.92
1.76
0.59
36.27
1.9697
81.35
2
7
36,37 1:7-20
0.00
0.00
28.55
1.63
0.55
30.73
2.0798
82.25
2
7
38,39 1:7-21
0.00
0.00
30.49
1.56
0.56
32.61
2.6705
101.05
2
7
33,34,35 2:7-19
0.00
0.00
31.15
1.45
0.56
33.16
3.4477
82.35
3
7
36,37 2:7-20
0.00
0.00
33.89
1.91
0.66
36.46
3.2646
82.75
3
7
38,39 2:7-21
0.00
0.00
33.22
1.48
0.61
35.30
4.3059
101.95
3
7
33,34,35 3:7-19
0.00
0.00
29.63
1.12
0.54
31.29
2.5117
83.5
2
7
36,37 3:7-20
0.00
0.00
30.02
1.59
0.60
32.21
2.4369
83.35
2
7
38,39 3:7-21
0.00
0.00
30.13
1.21
0.49
31.84
3.4107
103.2
2
AVERAGE
SD
total neutrol sterols excretion (mol/100 gr mouse/day)
33.33 46.02 36.80 44.14 49.92 46.73 48.62 47.62 46.28 44.38 5.60 53.67 68.64 36.83 53.80 70.10 45.70 50.36 54.51 46.03 53.29 10.66 45.43 38.85 54.48 53.24 50.74 58.33 53.40 40.40 51.99 49.65 6.64 53.10 49.10 62.75 76.95 66.63 80.94 62.10 71.35 74.80 66.41 10.76 65.30 62.80 46.18 64.00 67.20 44.79 54.10 62.25 38.54 56.13 10.56 53.73 39.82 49.32 56.74 41.17 48.46 57.86 42.69 62.46 50.25 8.01 43.91 38.86 43.10 46.28 47.94 49.70 47.06 47.08 52.61 46.28 3.98
coprostanol
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
% of neutral sterols
coprostanon cholesterol cholestanol
0.00
93.79
2.81
0.00
94.62
2.60
0.00
94.21
2.49
0.00
93.77
3.10
0.00
94.93
2.59
0.00
94.27
2.57
0.00
94.74
2.67
0.00
94.64
2.74
0.00
94.87
2.41
0.00
94.43
2.67
0.00
0.44
0.20
0.00
95.56
1.81
0.00
95.68
1.21
0.00
93.92
3.37
0.00
95.25
2.14
0.00
95.25
1.79
0.00
94.27
3.30
0.00
95.13
2.33
0.00
95.44
1.75
0.00
94.50
3.22
0.00
95.00
2.32
0.00
0.62
0.79
0.00
93.70
2.61
0.00
93.85
3.35
0.00
95.50
2.34
0.00
94.14
2.47
0.00
94.56
2.64
0.00
95.40
2.43
0.00
94.51
2.48
0.00
94.39
3.19
0.00
95.23
2.57
0.00
94.59
2.68
0.00
0.66
0.35
0.00
95.58
2.29
0.00
95.14
2.70
0.00
94.78
2.87
0.00
95.41
2.52
0.00
95.64
2.37
0.00
95.63
2.32
0.00
95.41
2.52
0.00
95.94
1.99
0.00
95.75
2.20
0.00
95.48
2.42
0.00
0.35
0.27
0.00
94.65
2.75
0.00
95.75
1.76
0.00
93.21
4.45
0.00
94.64
2.62
0.00
95.50
1.83
0.00
92.11
5.45
0.00
95.04
2.43
0.00
96.01
1.66
0.00
92.97
4.91
0.00
94.43
3.09
0.00
1.36
1.45
0.00
94.65
2.95
0.00
93.61
3.85
0.00
94.34
3.16
0.00
94.80
2.98
0.00
93.18
4.54
0.00
94.20
3.20
0.00
95.34
2.65
0.00
93.68
4.19
0.00
94.48
2.99
0.00
94.25
3.39
0.00
0.67
0.65
0.00
93.52
4.86
0.00
92.91
5.31
0.00
93.50
4.79
0.00
93.93
4.37
0.00
92.96
5.23
0.00
94.09
4.18
0.00
94.69
3.59
0.00
93.21
4.95
0.00
94.64
3.80
0.00
93.71
4.56
0.00
0.67
0.61
lathosterol
3.40 2.77 3.30 3.13 2.48 3.16 2.59 2.62 2.72 2.91 0.34 2.63 3.10 2.72 2.62 2.96 2.43 2.54 2.81 2.28 2.68 0.26 3.69 2.80 2.16 3.40 2.80 2.17 3.02 2.42 2.20 2.74 0.56 2.13 2.16 2.36 2.07 1.99 2.05 2.06 2.08 2.05 2.10 0.11 2.60 2.49 2.35 2.74 2.67 2.44 2.53 2.33 2.13 2.48 0.19 2.40 2.54 2.50 2.22 2.28 2.61 2.01 2.13 2.52 2.36 0.21 1.63 1.78 1.71 1.70 1.81 1.74 1.72 1.85 1.55 1.72 0.09
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Page A105 of A172
group
cage#
GC
neutral sterols (mol/g feces)
neutral sterols total feces average weight days
sample# campesterol stigmasterol b-sitosterol b-sitostanol total (mol/g feces) (g)
(g)
1
1,2
1:1-1
0.63
0.21
1.70
0.00
2.54
3.075
123.6
2
1
3,4
1:1-2
0.72
0.25
1.84
0.00
2.81
2.4897
87.25
2
1
5
1:1-3
0.60
0.20
1.51
0.00
2.31
2.2946
88.55
2
1
1,2
2:1-1
0.78
0.18
1.93
0.00
2.90
4.692
122.45
3
1
3,4
2:1-2
0.73
0.25
1.79
0.00
2.77
3.7789
86.55
3
1
5
2:1-3
0.75
0.24
1.94
0.00
2.93
3.4106
88.2
3
1
1,2
3:1-1
0.68
0.21
1.64
0.00
2.54
3.5754
121.65
2
1
3,4
3:1-2
0.66
0.16
1.64
0.00
2.46
2.8781
86.55
2
1
5
3:1-3
0.58
0.20
1.37
0.00
2.15
2.7589
88.05
2
AVERAGE
SD
2
6
1:2-4
0.69
0.25
1.80
0.00
2.74
2.7619
94.55
2
2
7,8
1:2-5
0.64
0.24
1.64
0.00
2.52
4.0581
126.6
2
2
9,10 1:2-6
0.73
0.25
1.92
0.00
2.91
2.0935
82.2
2
2
6
2:2-4
0.70
0.12
1.76
0.00
2.59
4.075
94.25
3
2
7,8
2:2-5
0.74
0.27
1.85
0.00
2.86
6.371
126.45
3
2
9,10 2:2-6
0.80
0.17
2.07
0.00
3.04
3.4074
82.1
3
2
6
3:2-4
0.70
0.23
1.69
0.00
2.62
2.9279
94
2
2
7,8
3:2-5
0.56
0.16
1.25
0.00
1.97
4.3985
126.6
2
2
9,10 3:2-6
0.72
0.25
1.81
0.00
2.78
2.4575
81.85
2
AVERAGE
SD
3
11,12,13 1:3-7
0.71
0.26
1.82
0.00
2.78
3.4597
118
2
3
15
1:3-8
0.67
0.24
1.69
0.00
2.60
2.2994
83.55
2
3
14,16,17 1:3-9
0.71
0.13
1.82
0.00
2.65
2.3454
82.8
2
3
11,12,13 2:3-7
0.74
0.26
1.84
0.00
2.83
5.8147
118.75
3
3
15
2:3-8
0.66
0.20
1.63
0.00
2.49
3.7718
83.5
3
3
14,16,17 2:3-9
0.71
0.13
1.85
0.00
2.70
3.7727
83.05
3
3
11,12,13 3:3-7
0.70
0.24
1.65
0.00
2.59
4.025
119.6
2
3
15
3:3-8
0.64
0.20
1.52
0.00
2.36
2.5265
84.2
2
3
14,16,17 3:3-9
0.64
0.14
1.56
0.00
2.33
2.6905
83.45
2
AVERAGE
SD
4
18
1:4-10
0.70
0.26
1.78
0.00
2.74
1.689
59.5
2
4
19
1:4-11
0.53
0.08
1.34
0.00
1.94
3.1432
116.4
2
4
20,21 1:4-12
0.66
0.09
1.63
0.00
2.38
3.2545
112.2
2
4
18
2:4-10
0.67
0.10
1.68
0.00
2.45
2.7133
58.85
3
4
19
2:4-11
0.71
0.10
1.68
0.00
2.49
4.6407
116.55
3
4
20,21 2:4-12
0.77
0.08
1.86
0.00
2.72
5.2075
111.85
3
4
18
3:4-10
0.54
0.07
1.25
0.00
1.86
2.041
58.7
2
4
19
3:4-11
0.66
0.08
1.53
0.00
2.27
3.4464
117.45
2
4
20,21 3:4-12
0.61
0.07
1.42
0.00
2.10
3.9549
112.9
2
AVERAGE
SD
5
22
1:5-13
0.70
0.13
1.80
0.00
2.64
3.195
110.7
2
5
24,25 1:5-14
0.59
0.24
1.50
0.00
2.32
2.9408
88.35
2
5
23,26,27 1:5-15
0.64
0.11
1.76
0.00
2.51
2.6399
84.6
2
5
22
2:5-13
0.72
0.11
1.74
0.00
2.57
4.9712
111.6
3
5
24,25 2:5-14
0.61
0.10
1.49
0.00
2.19
4.4833
88.5
3
5
23,26,27 2:5-15
0.79
0.14
1.97
0.00
2.90
3.767
85.55
3
5
22
3:5-13
0.59
0.22
1.47
0.00
2.27
3.6113
112.85
2
5
24,25 3:5-14
0.51
0.20
1.25
0.00
1.96
3.3522
88.9
2
5
23,26,27 3:5-15
0.58
0.11
1.36
0.00
2.04
2.7034
85.5
2
AVERAGE
SD
6
28,29 1:6-16
0.66
0.09
1.71
0.00
2.47
2.5036
87.1
2
6
31
1:6-17
0.68
0.09
1.71
0.00
2.48
2.7083
112.8
2
6
30,32 1:6-18
0.66
0.09
1.73
0.00
2.48
2.3554
85.4
2
6
28,29 2:6-16
0.60
0.10
1.55
0.00
2.25
3.9684
87.15
3
6
31
2:6-17
0.77
0.07
1.91
0.00
2.75
3.9678
112.45
3
6
30,32 2:6-18
0.62
0.09
1.59
0.00
2.29
3.5666
84.55
3
6
28,29 3:6-16
0.53
0.07
1.27
0.00
1.87
2.9076
87.65
2
6
31
3:6-17
0.59
0.07
1.36
0.00
2.02
3.2449
111.95
2
6
30,32 3:6-18
0.66
0.08
1.61
0.00
2.34
2.7643
84.65
2
AVERAGE
SD
7
33,34,35 1:7-19
0.75
0.10
1.91
0.00
2.76
1.9697
81.35
2
7
36,37 1:7-20
0.68
0.09
1.72
0.00
2.49
2.0798
82.25
2
7
38,39 1:7-21
0.72
0.09
1.77
0.00
2.57
2.6705
101.05
2
7
33,34,35 2:7-19
0.64
0.31
1.47
0.00
2.42
3.4477
82.35
3
7
36,37 2:7-20
0.78
0.10
1.84
0.00
2.73
3.2646
82.75
3
7
38,39 2:7-21
0.69
0.24
1.64
0.00
2.57
4.3059
101.95
3
7
33,34,35 3:7-19
0.59
0.07
1.38
0.00
2.04
2.5117
83.5
2
7
36,37 3:7-20
0.59
0.07
1.42
0.00
2.08
2.4369
83.35
2
7
38,39 3:7-21
0.60
0.05
1.39
0.00
2.05
3.4107
103.2
2
AVERAGE
SD
total neutrol sterols excretion (mol/100 gr mouse/day)
3.16 4.01 2.99 3.70 4.04 3.78 3.73 4.09 3.37
3.65 0.39
4.00 4.03 3.70 3.73 4.80 4.21 4.08 3.42 4.18
4.02 0.39
4.07 3.58 3.76 4.62 3.75 4.08 4.36 3.53 3.76
3.95 0.37
3.89 2.62 3.45 3.77 3.31 4.21 3.24 3.33 3.68
3.50 0.46
3.81 3.86 3.92 3.82 3.70 4.25 3.64 3.69 3.23
3.77 0.27
3.54 2.98 3.43 3.42 3.23 3.22 3.10 2.93 3.83
3.30 0.29
3.35 3.14 3.40 3.37 3.59 3.62 3.07 3.05 3.38
3.33 0.21
campesterol
24.96 25.48 25.94 27.00 26.27 25.66 26.91 26.93 26.95
26.23 0.76
25.12 25.53 25.18 27.14 25.78 26.27 26.64 28.32 26.02
26.22 1.03
25.38 25.76 26.71 26.04 26.43 26.48 26.94 27.07 27.51
26.48 0.67
25.58 27.20 27.65 27.46 28.49 28.28 28.79 28.92 29.11
27.94 1.11
26.55 25.24 25.40 27.78 27.70 27.10 25.86 25.80 28.26
26.63 1.12
26.89 27.35 26.66 26.85 28.05 26.95 28.47 28.98 28.13
27.59 0.83
27.15 27.35 27.87 26.37 28.77 26.78 28.82 28.54 29.25
27.88 1.02
% of neutral sterols
stigmasterol b-sitosterol
8.33
66.71
8.91
65.61
8.77
65.29
6.32
66.68
9.15
64.58
8.15
66.18
8.45
64.63
6.49
66.57
9.21
63.85
8.20
65.57
1.08
1.05
9.29
65.59
9.40
65.07
8.72
66.10
4.77
68.09
9.45
64.77
5.69
68.05
8.70
64.66
8.21
63.47
8.84
65.13
8.12
65.66
1.70
1.54
9.20
65.42
9.09
65.15
4.91
68.38
9.07
64.89
8.02
65.55
4.95
68.57
9.39
63.67
8.48
64.46
5.81
66.68
7.66
65.86
1.89
1.69
9.66
64.76
3.87
68.93
3.67
68.68
3.96
68.58
4.01
67.50
3.09
68.63
3.91
67.30
3.42
67.66
3.42
67.46
4.33
67.72
2.02
1.28
5.09
68.37
10.23
64.53
4.47
70.14
4.45
67.77
4.41
67.88
4.81
68.09
9.49
64.65
10.34
63.86
5.19
66.55
6.50
66.87
2.67
2.12
3.70
69.41
3.73
68.92
3.70
69.64
4.27
68.88
2.50
69.44
3.84
69.21
3.65
67.88
3.65
67.37
3.38
68.49
3.60
68.81
0.47
0.76
3.56
69.28
3.42
69.22
3.36
68.77
12.84
60.79
3.66
67.57
9.32
63.91
3.34
67.84
3.18
68.28
2.68
68.06
5.04
67.08
3.55
2.85
b-sitostanol
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
0.00 0.00
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Appendix to TNO-report of 3M-02
Final version
04-12-2006
sterols
total neutral sterols coprostanol coprostanon cholesterol cholestanol lathosterol
total phytosterols campesterol stigmasterol b-sitosterol b-sitostanol
sterols
total neutral sterols coprostanol coprostanon cholesterol cholestanol lathosterol
total phytosterols campesterol stigmasterol b-sitosterol b-sitostanol
sterols
total neutral sterols coprostanol coprostanon cholesterol cholestanol lathosterol
total phytosterols campesterol stigmasterol b-sitosterol b-sitostanol
group 1 vs 2 nn p 9 9 0.050 9 9 1.000 9 9 1.000 9 9 0.040 9 9 0.258 9 9 0.190 9 9 0.113 9 9 0.863 9 9 0.666 9 9 0.931 9 9 1.000 group 2 vs 4 nn p 9 9 0.040 9 9 1.000 9 9 1.000 9 9 0.077 9 9 0.489 9 9 <0.001 9 9 0.024 9 9 0.006 9 9 0.004 9 9 0.024 9 9 1.000 group 3 vs 7 nn p 9 9 0.190 9 9 1.000 9 9 1.000 9 9 0.031 9 9 <0.001 9 9 <0.001 9 9 <0.001 9 9 0.008 9 9 0.040 9 9 0.190 9 9 1.000
group 1 vs 3 nn p 9 9 0.077 9 9 1.000 9 9 1.000 9 9 0.863 9 9 0.546 9 9 0.489 9 9 0.222 9 9 0.546 9 9 0.863 9 9 1.000 9 9 1.000 group 2 vs 5 nn p 9 9 0.666 9 9 1.000 9 9 1.000 9 9 0.605 9 9 0.340 9 9 0.113 9 9 0.136 9 9 0.489 9 9 0.387 9 9 0.387 9 9 1.000 group 4 vs 5 nn p 9 9 0.113 9 9 1.000 9 9 1.000 9 9 0.094 9 9 0.605 9 9 <0.001 9 9 0.190 9 9 0.031 9 9 0.002 9 9 0.387 9 9 1.000
Sterol excretion, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
nn p nn p nn
p
9 9 <0.001 9 9 0.050 9 9 0.161
9 9 1.000 9 9 1.000 9 9 1.000
9 9 1.000 9 9 1.000 9 9 1.000
9 9 <0.001 9 9 0.546 9 9 0.489
9 9 0.050 9 9 1.000 9 9 0.002
9 9 <0.001 9 9 0.008 9 9 0.010
9 9 0.489 9 9 0.730 9 9 0.077
9 9 0.002 9 9 0.489 9 9 0.014
9 9 0.004 9 9 0.258 9 9 <0.001
9 9 0.001 9 9 0.190 9 9 <0.001
9 9 1.000 9 9 1.000 9 9 1.000
group 2 vs 6 group 2 vs 7
group 3 vs 4
nn p nn p nn
p
9 9 0.666 9 9 0.113 9 9 0.006
9 9 1.000 9 9 1.000 9 9 1.000
9 9 1.000 9 9 1.000 9 9 1.000
9 9 0.040 9 9 0.003 9 9 0.003
9 9 0.050 9 9 <0.001 9 9 0.136
9 9 0.008 9 9 <0.001 9 9 <0.001
9 9 0.001 9 9 <0.001 9 9 0.063
9 9 0.008 9 9 0.003 9 9 0.006
9 9 <0.001 9 9 0.031 9 9 0.004
9 9 <0.001 9 9 0.113 9 9 0.024
9 9 1.000 9 9 1.000 9 9 1.000
group 4 vs 6 group 4 vs 7
group 5 vs 6
nn p nn p nn
p
9 9 0.006 9 9 <0.001 9 9 0.190
9 9 1.000 9 9 1.000 9 9 1.000
9 9 1.000 9 9 1.000 9 9 1.000
9 9 <0.001 9 9 <0.001 9 9 0.489
9 9 <0.001 9 9 <0.001 9 9 0.258
9 9 0.011 9 9 <0.001 9 9 0.297
9 9 0.190 9 9 0.340 9 9 0.004
9 9 0.258 9 9 0.796 9 9 0.094
9 9 0.436 9 9 0.297 9 9 <0.001
9 9 0.040 9 9 0.730 9 9 0.024
9 9 1.000 9 9 1.000 9 9 1.000
group 1 vs 7 nn p 9 9 0.605 9 9 1.000 9 9 1.000 9 9 0.024 9 9 <0.001 9 9 <0.001 9 9 0.063 9 9 0.003 9 9 0.050 9 9 0.040 9 9 1.000 group 3 vs 5 nn p 9 9 0.161 9 9 1.000 9 9 1.000 9 9 0.730 9 9 0.931 9 9 0.387 9 9 0.546 9 9 0.730 9 9 0.436 9 9 0.546 9 9 1.000 group 5 vs 7 nn p 9 9 0.094 9 9 1.000 9 9 1.000 9 9 0.190 9 9 0.063 9 9 <0.001 9 9 0.001 9 9 0.031 9 9 0.024 9 9 0.605 9 9 1.000
group 2 vs 3 nn p 9 9 0.489 9 9 1.000 9 9 1.000 9 9 0.222 9 9 0.190 9 9 0.931 9 9 0.796 9 9 0.387 9 9 0.605 9 9 0.730 9 9 1.000 group 3 vs 6 nn p 9 9 0.863 9 9 1.000 9 9 1.000 9 9 0.340 9 9 0.008 9 9 0.190 9 9 0.001 9 9 0.014 9 9 <0.001 9 9 0.001 9 9 1.000 group 6 vs 7 nn p 9 9 0.297 9 9 1.000 9 9 1.000 9 9 0.113 9 9 0.004 9 9 <0.001 9 9 0.863 9 9 0.546 9 9 0.387 9 9 0.094 9 9 1.000
p<0.05
Page A106 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
E4. Fecal fatty acid measurements
1:1-1
weight feces
8.6
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
13257 256605
2203 231285 22933
1279 2731
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
2.5
13.9
1619
7.09
48.4
269.5
31340
122.23
0.4
2.3
269
1.06
43.6
242.9
28247
99.29
4.3
24.1
2801
9.91
0.2
1.3
156
0.56
0.5
2.9
334
1.20
total
530293
100
557.0
64766
241.33
C15:0
76166
1:1-2
weight feces
10.1
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
16324 404265
3337 411875 37807
2492 1091
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.9
14.8
1465
6.42
46.1
366.5
36288
141.53
0.4
3.0
300
1.18
47.0
373.4
36971
129.95
4.3
34.3
3394
12.01
0.3
2.3
224
0.80
0.1
1.0
98
0.35
total
877191
100
795.3
78739
292.24
C15:0
88241
1:1-3
weight feces
8.8
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
18959 482473
4337 520971 55946
3057 6151
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.7
14.2
1618
7.09
44.2
362.4
41183
160.62
0.4
3.3
370
1.46
47.7
391.3
44469
156.30
5.1
42.0
4775
16.90
0.3
2.3
261
0.93
0.6
4.6
525
1.89
total
1091894
100
820.2
93201
345.18
C15:0
106504
2:1-1
weight feces
7.6
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
8193
1.8
7.5
993
4.35
210520
45.4
193.9
25508
99.49
1596
0.3
1.5
193
0.76
214647
46.3
197.7
26008
91.42
24494
5.3
22.6
2968
10.51
1701
0.4
1.6
206
0.73
2635
0.6
2.4
319
1.15
total
463786
100
427.1
56196
208.40
C15:0
86874
2:1-2
weight feces
5.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
7613
1.8
6.7
1172
5.13
201403
46.8
176.8
31014
120.96
1437
0.3
1.3
221
0.87
196561
45.7
172.5
30268
106.39
19865
4.6
17.4
3059
10.83
1352
0.3
1.2
208
0.74
2147
0.5
1.9
331
1.19
total
430378
100
377.8
66274
246.11
C15:0
91143
2:1-3
weight feces
7.2
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
9801
1.4
9.1
1262
5.52
284195
41.3
263.4
36587
142.70
2725
0.4
2.5
351
1.38
348778
50.7
323.3
44901
157.83
35118
5.1
32.6
4521
16.00
2252
0.3
2.1
290
1.03
4534
0.7
4.2
584
2.10
total
687403
100
637.2
88496
326.56
C15:0
86307
molw
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
228.4 256.4 254.4 284.5 282.5 280.5 278.5
MYSTRIC ACID PALMITIC ACID PALMITOLEIC ACID STEARIC ACID OLEIC ACID LINOLEIC ACID LINOLENIC ACID
Page A107 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
3:1-1
weight feces
7.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
5463
1.2
7.5
1023
4.48
156085
35.1
213.4
29233
114.01
1332
0.3
1.8
249
0.98
248855
56.0
340.2
46607
163.82
26880
6.0
36.8
5034
17.82
1566
0.4
2.1
293
1.05
4158
0.9
5.7
779
2.80
total
444339
100
607.5
83219
304.96
C15:0
58514
3:1-2
weight feces
5.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
7866
1.5
7.3
1287
5.63
223765
43.6
208.6
36598
142.74
1702
0.3
1.6
278
1.09
248550
48.4
231.7
40651
142.89
26968
5.3
25.1
4411
15.61
1646
0.3
1.5
269
0.96
2844
0.6
2.7
465
1.67
total
513341
100
478.6
83959
310.59
C15:0
85813
3:1-3
weight feces
8.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
11108
1.5
9.5
1150
5.03
319405
42.1
274.4
33063
128.95
2509
0.3
2.2
260
1.02
380522
50.1
326.9
39390
138.45
37736
5.0
32.4
3906
13.83
2443
0.3
2.1
253
0.90
5057
0.7
4.3
523
1.88
total
758780
100
651.9
78546
290.07
C15:0
93112
1:2-4
weight feces
13.4
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
27893
1.2
20.7
1545
6.76
881387
39.5
654.1
48810
190.37
8053
0.4
6.0
446
1.75
1181866
53.0
877.0
65450
230.05
109775
4.9
81.5
6079
21.52
5394
0.2
4.0
299
1.06
17094
0.8
12.7
947
3.40
total
2231462
100
1655.9
123575
454.92
C15:0
107806
1:2-5
weight feces
9.2
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
31737
1.4
19.1
2081
9.11
923618
40.8
557.2
60560
236.19
93
0.0
0.1
6
0.02
1135022
50.2
684.7
74421
261.59
147397
6.5
88.9
9665
34.21
7383
0.3
4.5
484
1.73
16098
0.7
9.7
1056
3.79
total
2261348
100
1364.1
148272
546.64
C15:0
132620
1:2-6
weight feces
9
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
10087
1.7
6.6
728
3.19
262021
45.2
170.1
18905
73.73
2302
0.4
1.5
166
0.65
272616
47.0
177.0
19669
69.14
27573
4.8
17.9
1989
7.04
1825
0.3
1.2
132
0.47
3059
0.5
2.0
221
0.79
total
579483
100
376.3
41810
155.01
C15:0
123199
Page A108 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:2-4
weight feces
9.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
18615
1.6
15.3
1579
6.91
488224
42.0
401.8
41421
161.55
4357
0.4
3.6
370
1.45
581814
50.0
478.8
49361
173.50
60179
5.2
49.5
5106
18.07
3098
0.3
2.5
263
0.94
7073
0.6
5.8
600
2.15
total
1163360
100
957.4
98699
364.58
C15:0
97212
2:2-5
weight feces
9.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
18128
1.3
14.7
1575
6.90
549910
39.1
444.5
47793
186.40
5378
0.4
4.3
467
1.84
733331
52.1
592.7
63734
224.02
85796
6.1
69.3
7457
26.39
4289
0.3
3.5
373
1.33
10396
0.7
8.4
904
3.24
total
1407228
100
1137.4
122302
450.12
C15:0
98978
2:2-6
weight feces
5.9
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
5981
2.7
4.9
824
3.61
103637
47.0
84.2
14272
55.66
790
0.4
0.6
109
0.43
97059
44.0
78.9
13366
46.98
10984
5.0
8.9
1513
5.35
884
0.4
0.7
122
0.43
1019
0.5
0.8
140
0.50
total
220354
100
179.0
30345
112.97
C15:0
98463
3:2-4
weight feces
8.6
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
18311
1.7
15.1
1754
7.68
420511
39.9
346.4
40284
157.11
3649
0.3
3.0
350
1.37
545499
51.7
449.4
52257
183.68
56501
5.4
46.5
5413
19.16
3001
0.3
2.5
287
1.02
7025
0.7
5.8
673
2.42
total
1054497
100
868.7
101017
372.45
C15:0
97105
3:2-5
weight feces
7.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
14837
1.3
11.7
1515
6.63
453886
39.8
356.9
46352
180.78
4567
0.4
3.6
466
1.83
591094
51.9
464.8
60364
212.18
63874
5.6
50.2
6523
23.09
3296
0.3
2.6
337
1.20
7991
0.7
6.3
816
2.93
total
1139545
100
896.1
116374
428.65
C15:0
101736
3:2-6
weight feces
8.4
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
5585
1.4
4.9
585
2.56
169177
42.5
148.8
17709
69.07
1364
0.3
1.2
143
0.56
196952
49.5
173.2
20617
72.47
20806
5.2
18.3
2178
7.71
1530
0.4
1.3
160
0.57
2302
0.6
2.0
241
0.87
total
397716
100
349.7
41633
153.80
C15:0
90981
Page A109 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:3-7
weight feces
11
mg
2:3-7
weight feces
8.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
14517 354931
3055 369554 36484
2417 4404
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.8
10.1
914
4.00
45.2
245.8
22349
87.16
0.4
2.1
192
0.76
47.1
256.0
23269
81.79
4.6
25.3
2297
8.13
0.3
1.7
152
0.54
0.6
3.1
277
1.00
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
7895 202071
1616 212839 21090
1476 2397
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.8
6.9
833
3.65
45.0
177.0
21327
83.18
0.4
1.4
171
0.67
47.4
186.4
22464
78.96
4.7
18.5
2226
7.88
0.3
1.3
156
0.56
0.5
2.1
253
0.91
total
785362
100
544.0
49451
183.38
total
449384
100
393.7
47429
175.80
C15:0
115502
C15:0
91324
1:3-8
weight feces
11.1
mg
2:3-8
weight feces
7.6
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
20655 613614
5769 774403 72889
4402 10243
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.4
11.8
1062
4.65
40.9
350.2
31548
123.04
0.4
3.3
297
1.17
51.6
441.9
39815
139.95
4.9
41.6
3747
13.27
0.3
2.5
226
0.81
0.7
5.8
527
1.89
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
13707 374445
3351 433392 46483
2770 5202
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.6
9.9
1300
5.69
42.6
269.9
35519
138.53
0.4
2.4
318
1.25
49.3
312.4
41111
144.50
5.3
33.5
4409
15.61
0.3
2.0
263
0.94
0.6
3.8
493
1.77
total
1501975
100
857.2
77221
284.77
total
879350
100
633.9
83414
308.29
C15:0
140182
C15:0
110969
1:3-9
weight feces
13.4
mg
2:3-9
weight feces
7.4
mg
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
C14:0
26223
1.2
14.5
1081
4.73
C16:0
851207
38.6
470.3
35098
136.89
C16:1
7949
0.4
4.4
328
1.29
C18:0
1186581
53.8
655.6
48927
171.97
C18:1
108707
4.9
60.1
4482
15.87
C18:2
7434
0.3
4.1
307
1.09
C18:3
16281
0.7
9.0
671
2.41
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
9500 267057
2386 302932 29071
2020 3014
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.5
7.5
1014
4.44
43.4
210.9
28499
111.15
0.4
1.9
255
1.00
49.2
239.2
32327
113.63
4.7
23.0
3102
10.98
0.3
1.6
216
0.77
0.5
2.4
322
1.15
total
2204382
100
1218.0
90894
334.26
total
615980
100
486.4
65734
243.12
C15:0
144789
C15:0
101306
Page A110 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
3:3-7
weight feces
8.5
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
7543 193432
1421 205498 21652
1375 2581
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.7
6.4
753
3.30
44.6
164.2
19312
75.32
0.3
1.2
142
0.56
47.4
174.4
20517
72.12
5.0
18.4
2162
7.65
0.3
1.2
137
0.49
0.6
2.2
258
0.93
total
433502
100
367.9
43281
160.36
C15:0
94269
3:3-8
weight feces
6.5
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
6822 183086
1710 197892 21000
1164 2282
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.6
6.4
987
4.32
44.2
172.2
26486
103.30
0.4
1.6
247
0.97
47.8
186.1
28628
100.63
5.1
19.7
3038
10.75
0.3
1.1
168
0.60
0.6
2.1
330
1.19
total
413956
100
389.3
59885
221.76
C15:0
85077
3:3-9
weight feces
14
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
13974 420919
4038 511096 49923
3410 6144
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.4
12.4
883
3.87
41.7
372.5
26609
103.78
0.4
3.6
255
1.00
50.6
452.3
32310
113.57
4.9
44.2
3156
11.17
0.3
3.0
216
0.77
0.6
5.4
388
1.39
total
1009504
100
893.5
63818
235.56
C15:0
90391
1:4-10
weight feces
8.2
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
16048
1.4
11.1
1350
5.91
495415
41.9
341.8
41686
162.58
3727
0.3
2.6
314
1.23
599026
50.7
413.3
50404
177.17
56734
4.8
39.1
4774
16.90
3322
0.3
2.3
280
1.00
7505
0.6
5.2
631
2.27
total
1181777
100
815.4
99439
367.06
C15:0
115946
1:4-11
weight feces
8.8
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
13693
1.5
8.5
966
4.23
392497
42.8
243.6
27681
107.96
3202
0.3
2.0
226
0.89
454998
49.7
282.4
32089
112.79
43482
4.7
27.0
3067
10.86
2667
0.3
1.7
188
0.67
5468
0.6
3.4
386
1.38
total
916007
100
568.5
64603
238.78
C15:0
128901
1:4-12
weight feces
9.8
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
11857
1.3
12.4
1261
5.52
361984
40.3
377.2
38489
150.11
2570
0.3
2.7
273
1.07
460388
51.3
479.7
48952
172.06
51833
5.8
54.0
5511
19.51
3265
0.4
3.4
347
1.24
5863
0.7
6.1
623
2.24
total
897760
100
935.5
95457
351.76
C15:0
76774
Page A111 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
2:4-10
weight feces
7.1
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
10522
1.2
8.5
1196
5.23
344618
40.8
278.0
39156
152.71
2591
0.3
2.1
294
1.16
438053
51.9
353.4
49772
174.94
40213
4.8
32.4
4569
16.17
2602
0.3
2.1
296
1.05
5292
0.6
4.3
601
2.16
total
843891
100
680.8
95883
353.44
C15:0
99169
2:4-11
weight feces
8.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
13021
1.5
10.2
1170
5.12
381006
42.6
297.9
34238
133.53
3180
0.4
2.5
286
1.12
444595
49.7
347.6
39952
140.43
44296
5.0
34.6
3981
14.09
2954
0.3
2.3
265
0.95
4928
0.6
3.9
443
1.59
total
893980
100
698.9
80335
296.84
C15:0
102328
2:4-12
weight feces
7.2
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
12246
1.6
9.2
1273
5.58
332981
44.3
249.3
34627
135.05
2528
0.3
1.9
263
1.03
357246
47.5
267.5
37150
130.58
40701
5.4
30.5
4232
14.98
2826
0.4
2.1
294
1.05
3646
0.5
2.7
379
1.36
total
752174
100
563.2
78218
289.63
C15:0
106848
3:4-10
weight feces
8.5
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
10172
1.3
9.5
1112
4.87
310431
40.9
288.5
33946
132.39
2449
0.3
2.3
268
1.05
394129
51.9
366.3
43098
151.49
34766
4.6
32.3
3802
13.46
2260
0.3
2.1
247
0.88
4580
0.6
4.3
501
1.80
total
758787
100
705.3
82973
305.94
C15:0
86070
3:4-11
weight feces
9.8
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
20169
1.9
17.5
1789
7.83
422311
39.3
367.1
37462
146.11
3653
0.3
3.2
324
1.27
565589
52.6
491.7
50171
176.35
52273
4.9
45.4
4637
16.41
2913
0.3
2.5
258
0.92
7488
0.7
6.5
664
2.39
total
1074396
100
934.0
95305
351.28
C15:0
92026
3:4-12
weight feces
10.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
15056
1.1
13.7
1283
5.62
501243
38.2
456.9
42703
166.55
4206
0.3
3.8
358
1.41
708410
54.0
645.8
60353
212.14
68397
5.2
62.3
5827
20.63
4333
0.3
3.9
369
1.32
9322
0.7
8.5
794
2.85
total
1310967
100
1195.1
111688
410.51
C15:0
87759
Page A112 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:5-13
weight feces
8.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
13526 393457
3030 473949 44962
3228 5563
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.4
9.5
1140
4.99
42.0
275.2
33162
129.34
0.3
2.1
255
1.00
50.5
331.6
39947
140.41
4.8
31.5
3790
13.41
0.3
2.3
272
0.97
0.6
3.9
469
1.68
total
937715
100
656.0
79035
291.81
C15:0
114357
1:5-14
weight feces
11
mg
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
C14:0
33483
1.3
19.7
1792
7.84
C16:0
1006010
39.7
592.1
53832
209.95
C16:1
9704
0.4
5.7
519
2.04
C18:0
1300166
51.3
765.3
69572
244.54
C18:1
160875
6.3
94.7
8608
30.47
C18:2
7669
0.3
4.5
410
1.46
C18:3
18973
0.7
11.2
1015
3.65
total
2536880
100
1493.2
135749
499.96
C15:0
135913
1:5-15
weight feces
8.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
11073 294462
2464 315545 28429
1608 3367
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.7
7.3
884
3.87
44.8
195.1
23500
91.65
0.4
1.6
197
0.77
48.0
209.0
25183
88.52
4.3
18.8
2269
8.03
0.2
1.1
128
0.46
0.5
2.2
269
0.96
total
656948
100
435.2
52429
194.27
C15:0
120773
2:5-13
weight feces
8.1
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
13428 386269
3253 437237 45693
2448 5034
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.5
10.8
1331
5.83
43.2
310.0
38276
149.28
0.4
2.6
322
1.27
48.9
350.9
43327
152.29
5.1
36.7
4528
16.03
0.3
2.0
243
0.86
0.6
4.0
499
1.79
total
893362
100
717.1
88525
327.35
C15:0
99670
2:5-14
weight feces
9.9
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
23143 686470
6696 830835 105595
4899 10267
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.4
17.1
1731
7.58
41.2
508.5
51359
200.31
0.4
5.0
501
1.97
49.8
615.4
62160
218.49
6.3
78.2
7900
27.97
0.3
3.6
367
1.31
0.6
7.6
768
2.76
total
1667905
100
1235.4
124787
460.38
C15:0
108008
2:5-15
weight feces
8.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
7030 128252
1016 106592 11903
760 1127
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
2.7
6.9
833
3.65
50.0
126.1
15190
59.24
0.4
1.0
120
0.47
41.5
104.8
12624
44.37
4.6
11.7
1410
4.99
0.3
0.7
90
0.32
0.4
1.1
133
0.48
total
256680
100
252.3
30401
113.53
C15:0
81381
Page A113 of A172
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Final version
04-12-2006
3:5-13
weight feces
6.8
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
26211 674622
5831 907134 95082
4891 12523
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.5
10.5
1538
6.73
39.1
269.2
39589
154.40
0.3
2.3
342
1.35
52.5
362.0
53233
187.11
5.5
37.9
5580
19.75
0.3
2.0
287
1.02
0.7
5.0
735
2.64
total
1726294
100
688.9
101304
373.01
C15:0
200479
3:5-14
weight feces
5.3
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
12309 339628
3302 368609 45053
2365 4243
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.6
10.3
1940
8.49
43.8
283.7
53533
208.79
0.4
2.8
520
2.05
47.5
307.9
58101
204.22
5.8
37.6
7101
25.14
0.3
2.0
373
1.33
0.5
3.5
669
2.40
total
775509
100
647.9
122237
452.42
C15:0
95763
3:5-15
weight feces
8.1
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
4597 119586
872 128120 13678
939 1419
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.7
4.7
584
2.56
44.4
123.1
15199
59.28
0.3
0.9
111
0.44
47.6
131.9
16283
57.23
5.1
14.1
1738
6.15
0.3
1.0
119
0.43
0.5
1.5
180
0.65
total
269211
100
277.1
34215
126.73
C15:0
77711
1:6-16
weight feces
11.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
20396
1.2
12.5
1070
4.69
664144
40.5
407.7
34849
135.92
5241
0.3
3.2
275
1.08
854148
52.1
524.4
44819
157.54
80539
4.9
49.4
4226
14.96
5034
0.3
3.1
264
0.94
11097
0.7
6.8
582
2.09
total
1640599
100
1007.2
86086
317.21
C15:0
130309
1:6-17
weight feces
8.9
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
12220
1.4
8.4
939
4.11
366294
42.7
250.4
28137
109.74
3077
0.4
2.1
236
0.93
427401
49.9
292.2
32831
115.40
40324
4.7
27.6
3098
10.96
2415
0.3
1.7
186
0.66
5166
0.6
3.5
397
1.42
total
856897
100
585.8
65824
243.23
C15:0
117016
1:6-18
weight feces
10.8
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
17671
1.4
10.4
962
4.21
535579
41.3
315.0
29168
113.76
4357
0.3
2.6
237
0.93
659139
50.8
387.7
35897
126.18
67882
5.2
39.9
3697
13.09
4052
0.3
2.4
221
0.79
8388
0.6
4.9
457
1.64
total
1297068
100
762.9
70639
260.59
C15:0
136015
Page A114 of A172
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Final version
04-12-2006
2:6-16
weight feces
12.1
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
16368
1.4
12.4
1028
4.50
492848
43.0
374.7
30966
120.77
3736
0.3
2.8
235
0.92
567463
49.5
431.4
35654
125.32
55948
4.9
42.5
3515
12.44
3689
0.3
2.8
232
0.83
6084
0.5
4.6
382
1.37
total
1146136
100
871.4
72013
266.16
C15:0
105228
2:6-17
weight feces
8.5
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
7619
2.2
7.2
846
3.70
165470
48.1
156.1
18369
71.64
1010
0.3
1.0
112
0.44
151890
44.1
143.3
16862
59.27
15484
4.5
14.6
1719
6.08
1040
0.3
1.0
115
0.41
1620
0.5
1.5
180
0.65
total
344133
100
324.7
38204
142.20
C15:0
84780
2:6-18
weight feces
10.2
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
13778
1.8
9.5
928
4.06
361552
46.5
248.5
24363
95.02
2752
0.4
1.9
185
0.73
355381
45.7
244.3
23948
84.17
38817
5.0
26.7
2616
9.26
2125
0.3
1.5
143
0.51
3865
0.5
2.7
260
0.94
total
778270
100
534.9
52444
194.69
C15:0
116392
3:6-16
weight feces
9.6
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
11600
1.2
10.6
1109
4.86
381872
39.5
350.5
36507
142.38
3071
0.3
2.8
294
1.15
510655
52.8
468.7
48818
171.59
50174
5.2
46.0
4797
16.98
2963
0.3
2.7
283
1.01
6403
0.7
5.9
612
2.20
total
966738
100
887.2
92420
340.17
C15:0
87169
3:6-17
weight feces
7.6
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
6657
1.8
6.5
849
3.72
174284
45.8
168.9
22222
86.67
1200
0.3
1.2
153
0.60
175484
46.1
170.0
22375
78.65
19588
5.2
19.0
2498
8.84
1351
0.4
1.3
172
0.61
1768
0.5
1.7
225
0.81
total
380332
100
368.6
48494
179.90
C15:0
82557
3:6-18
weight feces
8.1
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
8700
1.3
8.3
1022
4.47
277658
41.8
264.1
32606
127.17
1872
0.3
1.8
220
0.86
334779
50.4
318.4
39313
138.18
34667
5.2
33.0
4071
14.41
1792
0.3
1.7
210
0.75
4339
0.7
4.1
510
1.83
total
663807
100
631.4
77952
287.68
C15:0
84105
Page A115 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
1:7-19
weight feces
10.7
mg
2:7-19
weight feces
12.4
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
9334 205542
1575 189750 23168
1823 1777
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
2.2
6.0
557
2.44
47.5
131.2
12263
47.83
0.4
1.0
94
0.37
43.8
121.1
11321
39.79
5.4
14.8
1382
4.89
0.4
1.2
109
0.39
0.4
1.1
106
0.38
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
10569 262993
1907 260336 26561
1861 2435
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.9
8.5
689
3.02
46.4
212.5
17137
66.84
0.3
1.5
124
0.49
45.9
210.3
16963
59.63
4.7
21.5
1731
6.13
0.3
1.5
121
0.43
0.4
2.0
159
0.57
total
432969
100
276.4
25831
96.09
total
566662
100
457.9
36924
137.09
C15:0
125320
C15:0
99012
1:7-20
weight feces
7.2
mg
2:7-20
weight feces
6
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
5300 116241
798 106821 14034
1332 1059
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
2.2
3.9
545
2.39
47.3
86.1
11953
46.62
0.3
0.6
82
0.32
43.5
79.1
10984
38.61
5.7
10.4
1443
5.11
0.5
1.0
137
0.49
0.4
0.8
109
0.39
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
4357 90363
573 77962 9578
799 815
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
2.4
3.7
608
2.66
49.0
75.7
12617
49.21
0.3
0.5
80
0.31
42.3
65.3
10886
38.26
5.2
8.0
1337
4.73
0.4
0.7
112
0.40
0.4
0.7
114
0.41
total
245585
100
181.8
25254
93.92
total
184447
100
154.5
25754
95.99
C15:0
108053
C15:0
95490
1:7-21
weight feces
10
mg
2:7-21
weight feces
8.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
9522 270616
2338 313018 37006
3195 3428
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.5
6.2
622
2.72
42.3
176.8
17684
68.97
0.4
1.5
153
0.60
49.0
204.6
20455
71.90
5.8
24.2
2418
8.56
0.5
2.1
209
0.74
0.5
2.2
224
0.80
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
7650 212450
1476 217370 22360
1545 2300
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.6
5.6
642
2.81
45.7
155.1
17829
69.54
0.3
1.1
124
0.49
46.7
158.7
18242
64.12
4.8
16.3
1876
6.64
0.3
1.1
130
0.46
0.5
1.7
193
0.69
total
639123
100
417.7
41766
154.30
total
465151
100
339.6
39036
144.75
C15:0
122421
C15:0
109572
Page A116 of A172
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Final version
3:7-19
weight feces
5.8
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
4977 125240
723 123021 11441
691 1184
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.9
4.8
822
3.60
46.9
120.0
20682
80.66
0.3
0.7
119
0.47
46.0
117.8
20316
71.41
4.3
11.0
1889
6.69
0.3
0.7
114
0.41
0.4
1.1
196
0.70
total
267277
100
256.0
44138
163.94
C15:0
83524
3:7-20
weight feces
7.7
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
5155 125067
811 121012 14616
1143 1266
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.9
4.2
548
2.40
46.5
102.5
13306
51.90
0.3
0.7
86
0.34
45.0
99.1
12875
45.25
5.4
12.0
1555
5.50
0.4
0.9
122
0.43
0.5
1.0
135
0.48
total
269070
100
220.4
28627
106.31
C15:0
97655
3:7-21
weight feces
17
mg
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
area
11944 374593
3142 460518 44662
3197 5430
%
g fatty ng fatty acid nmol fatty acid/
acid
mg feces
mg feces
1.3
10.3
603
2.64
41.5
321.7
18926
73.82
0.3
2.7
159
0.62
51.0
395.5
23268
81.78
4.9
38.4
2257
7.99
0.4
2.7
162
0.58
0.6
4.7
274
0.99
total
903486
100
776.0
45648
168.41
C15:0
93140
04-12-2006
Page A117 of A172
Page A118 of A172
group
1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 AVERAGE SD
cage#
1,2 3,4 5 1,2 3,4 5 1,2 3,4 5
GC sample#
1:1-1 1:1-2 1:1-3 2:1-1 2:1-2 2:1-3 3:1-1 3:1-2 3:1-3
C14:0
7.09 6.42 7.09 4.35 5.13 5.52 4.48 5.63 5.03
fatty acids (mol/g feces) C16:0 C16:1 C18:0 C18:1
122.23 1.06 99.29 9.91 141.53 1.18 129.95 12.01 160.62 1.46 156.30 16.90 99.49 0.76 91.42 10.51 120.96 0.87 106.39 10.83 142.70 1.38 157.83 16.00 114.01 0.98 163.82 17.82 142.74 1.09 142.89 15.61 128.95 1.02 138.45 13.83
C18:2
0.56 0.80 0.93 0.73 0.74 1.03 1.05 0.96 0.90
C18:3
1.20 0.35 1.89 1.15 1.19 2.10 2.80 1.67 1.88
fatty acids total (mol/g feces)
241.33 292.24 345.18 208.40 246.11 326.56 304.96 310.59 290.07
6
1:2-4 6.76 190.37 1.75 230.05 21.52 1.06 3.40
7,8
1:2-5 9.11 236.19 0.02 261.59 34.21 1.73 3.79
9,10 1:2-6 3.19 73.73 0.65 69.14 7.04 0.47 0.79
6
2:2-4 6.91 161.55 1.45 173.50 18.07 0.94 2.15
7,8
2:2-5 6.90 186.40 1.84 224.02 26.39 1.33 3.24
9,10 2:2-6 3.61 55.66 0.43 46.98 5.35 0.43 0.50
6
3:2-4 7.68 157.11 1.37 183.68 19.16 1.02 2.42
7,8
3:2-5 6.63 180.78 1.83 212.18 23.09 1.20 2.93
9,10 3:2-6 2.56 69.07 0.56 72.47 7.71 0.57 0.87
454.92 546.64 155.01 364.58 450.12 112.97 372.45 428.65 153.80
11,12,13 15
14,16,17 11,12,13
15 14,16,17 11,12,13
15 14,16,17
1:3-7 1:3-8 1:3-9 2:3-7 2:3-8 2:3-9 3:3-7 3:3-8 3:3-9
4.00 87.16 0.76 81.79 8.13 0.54 1.00 4.65 123.04 1.17 139.95 13.27 0.81 1.89 4.73 136.89 1.29 171.97 15.87 1.09 2.41 3.65 83.18 0.67 78.96 7.88 0.56 0.91 5.69 138.53 1.25 144.50 15.61 0.94 1.77 4.44 111.15 1.00 113.63 10.98 0.77 1.15 3.30 75.32 0.56 72.12 7.65 0.49 0.93 4.32 103.30 0.97 100.63 10.75 0.60 1.19 5.52 150.11 1.07 172.06 19.51 1.24 2.24
183.38 284.77 334.26 175.80 308.29 243.12 160.36 221.76 351.76
18 19 20,21 18 19 20,21 18 19 20,21
1:4-10 5.91 162.58 1.23 177.17 16.90 1.00 2.27 1:4-11 4.23 107.96 0.89 112.79 10.86 0.67 1.38 1:4-12 5.52 150.11 1.07 172.06 19.51 1.24 2.24 2:4-10 5.23 152.71 1.16 174.94 16.17 1.05 2.16 2:4-11 5.12 133.53 1.12 140.43 14.09 0.95 1.59 2:4-12 5.58 135.05 1.03 130.58 14.98 1.05 1.36 3:4-10 4.87 132.39 1.05 151.49 13.46 0.88 1.80 3:4-11 7.83 146.11 1.27 176.35 16.41 0.92 2.39 3:4-12 5.62 166.55 1.41 212.14 20.63 1.32 2.85
367.06 238.78 351.76 353.44 296.84 289.63 305.94 351.28 410.51
22
1:5-13 4.99 129.34 1.00 140.41 13.41 0.97 1.68
24,25 1:5-14 7.84 209.95 2.04 244.54 30.47 1.46 3.65
23,26,27 1:5-15 3.87 91.65 0.77 88.52 8.03 0.46 0.96
22
2:5-13 5.83 149.28 1.27 152.29 16.03 0.86 1.79
24,25 2:5-14 7.58 200.31 1.97 218.49 27.97 1.31 2.76
23,26,27 2:5-15 3.65 59.24 0.47 44.37 4.99 0.32 0.48
22
3:5-13 6.73 154.40 1.35 187.11 19.75 1.02 2.64
24,25 3:5-14 8.49 208.79 2.05 204.22 25.14 1.33 2.40
23,26,27 3:5-15 2.56 59.28 0.44 57.23 6.15 0.43 0.65
291.81 499.96 194.27 327.35 460.38 113.53 373.01 452.42 126.73
28,29 31
30,32 28,29
31 30,32 28,29
31 30,32
1:6-16 4.69 135.92 1.08 157.54 14.96 0.94 2.09 1:6-17 4.11 109.74 0.93 115.40 10.96 0.66 1.42 1:6-18 4.21 113.76 0.93 126.18 13.09 0.79 1.64 2:6-16 4.50 120.77 0.92 125.32 12.44 0.83 1.37 2:6-17 3.70 71.64 0.44 59.27 6.08 0.41 0.65 2:6-18 4.06 95.02 0.73 84.17 9.26 0.51 0.94 3:6-16 4.86 142.38 1.15 171.59 16.98 1.01 2.20 3:6-17 3.72 86.67 0.60 78.65 8.84 0.61 0.81 3:6-18 4.47 127.17 0.86 138.18 14.41 0.75 1.83
317.21 243.23 260.59 266.16 142.20 194.69 340.17 179.90 287.68
33,34,35 1:7-19 2.44 47.83 0.37 39.79 4.89 0.39 0.38 36,37 1:7-20 2.39 46.62 0.32 38.61 5.11 0.49 0.39 38,39 1:7-21 2.72 68.97 0.60 71.90 8.56 0.74 0.80
33,34,35 2:7-19 3.02 66.84 0.49 59.63 6.13 0.43 0.57 36,37 2:7-20 2.66 49.21 0.31 38.26 4.73 0.40 0.41 38,39 2:7-21 2.81 69.54 0.49 64.12 6.64 0.46 0.69
33,34,35 3:7-19 3.60 80.66 0.47 71.41 6.69 0.41 0.70 36,37 3:7-20 2.40 51.90 0.34 45.25 5.50 0.43 0.48 38,39 3:7-21 2.64 73.82 0.62 81.78 7.99 0.58 0.99
96.09 93.92 154.30 137.09 95.99 144.75 163.94 106.31 168.41
total feces average weight days
(g)
(g)
3.075
123.6
2
2.4897
87.25
2
2.2946
88.55
2
4.692
122.45
3
3.7789
86.55
3
3.4106
88.2
3
3.5754
121.65
2
2.8781
86.55
2
2.7589
88.05
2
2.7619
94.55
2
4.0581
126.6
2
2.0935
82.2
2
4.075
94.25
3
6.371
126.45
3
3.4074
82.1
3
2.9279
94
2
4.3985
126.6
2
2.4575
81.85
2
3.4597
118
2
2.2994
83.55
2
2.3454
82.8
2
5.8147
118.75
3
3.7718
83.5
3
3.7727
83.05
3
4.025
119.6
2
2.5265
84.2
2
2.6905
83.45
2
1.689
59.5
2
3.1432
116.4
2
3.2545
112.2
2
2.7133
58.85
3
4.6407
116.55
3
5.2075
111.85
3
2.041
58.7
2
3.4464
117.45
2
3.9549
112.9
2
3.195
110.7
2
2.9408
88.35
2
2.6399
84.6
2
4.9712
111.6
3
4.4833
88.5
3
3.767
85.55
3
3.6113
112.85
2
3.3522
88.9
2
2.7034
85.5
2
2.5036
87.1
2
2.7083
112.8
2
2.3554
85.4
2
3.9684
87.15
3
3.9678
112.45
3
3.5666
84.55
3
2.9076
87.65
2
3.2449
111.95
2
2.7643
84.65
2
1.9697
81.35
2
2.0798
82.25
2
2.6705
101.05
2
3.4477
82.35
3
3.2646
82.75
3
4.3059
101.95
3
2.5117
83.5
2
2.4369
83.35
2
3.4107
103.2
2
total fatty acid excretion (mol/100 gr mouse/day)
300.20 416.95 447.24 266.18 358.19 420.92 448.15 516.42 454.44
403.19 80.09
664.43 876.12 197.40 525.43 755.96 156.29 580.05 744.63 230.89
525.69 268.58
268.83 391.86 473.41 286.94 464.20 368.14 269.83 332.70 567.05
380.33 103.97
520.97 322.39 510.16 543.18 393.97 449.48 531.88 515.39 719.00
500.71 110.09
421.11 832.08 303.10 486.06 777.41 166.63 596.83 852.97 200.35
515.17 265.81
455.90 292.00 359.37 403.99 167.25 273.76 564.22 260.72 469.72
360.77 124.47
116.33 118.75 203.89 191.32 126.23 203.79 246.56 155.41 278.30
182.29 57.68
C14:0
2.94 2.20 2.05 2.09 2.09 1.69 1.47 1.81 1.74
2.01 0.42
1.49 1.67 2.06 1.90 1.53 3.19 2.06 1.55 1.66
1.90 0.53
2.18 1.63 1.42 2.08 1.85 1.83 2.06 1.95 1.57
1.84 0.26
1.61 1.77 1.57 1.48 1.73 1.93 1.59 2.23 1.37
1.70 0.26
1.71 1.57 1.99 1.78 1.65 3.21 1.81 1.88 2.02
1.96 0.49
1.48 1.69 1.62 1.69 2.60 2.09 1.43 2.07 1.55
1.80 0.38
2.54 2.54 1.77 2.20 2.77 1.94 2.20 2.26 1.57
2.20 0.39
C16:0
50.65 48.43 46.53 47.74 49.15 43.70 37.39 45.96 44.46
46.00 3.91
41.85 43.21 47.57 44.31 41.41 49.27 42.18 42.17 44.91
44.10 2.74
47.53 43.21 40.95 47.31 44.93 45.72 46.97 46.58 42.68
45.10 2.33
44.29 45.21 42.68 43.21 44.99 46.63 43.27 41.59 40.57
43.60 1.89
44.32 41.99 47.18 45.60 43.51 52.18 41.39 46.15 46.77
45.46 3.24
42.85 45.12 43.65 45.38 50.38 48.81 41.86 48.18 44.20
45.60 2.90
49.77 49.63 44.70 48.75 51.26 48.04 49.20 48.81 43.83
48.22 2.42
% of fatty acids C16:1 C18:0 C18:1
0.44 41.14 4.11 0.40 44.47 4.11 0.42 45.28 4.90 0.36 43.87 5.04 0.35 43.23 4.40 0.42 48.33 4.90 0.32 53.72 5.84 0.35 46.00 5.03 0.35 47.73 4.77
0.38 45.97 4.79 0.04 3.65 0.54
0.39 50.57 4.73 0.00 47.85 6.26 0.42 44.60 4.54 0.40 47.59 4.96 0.41 49.77 5.86 0.38 41.59 4.74 0.37 49.32 5.14 0.43 49.50 5.39 0.36 47.12 5.01
0.35 47.54 5.18 0.13 2.86 0.57
0.41 44.60 4.43 0.41 49.14 4.66 0.39 51.45 4.75 0.38 44.91 4.48 0.41 46.87 5.06 0.41 46.74 4.52 0.35 44.97 4.77 0.44 45.38 4.85 0.31 48.92 5.55
0.39 47.00 4.79 0.04 2.37 0.35
0.34 48.27 4.60 0.37 47.24 4.55 0.31 48.92 5.55 0.33 49.50 4.58 0.38 47.31 4.75 0.36 45.09 5.17 0.34 49.52 4.40 0.36 50.20 4.67 0.34 51.68 5.02
0.35 48.63 4.81 0.02 1.93 0.37
0.34 48.12 4.60 0.41 48.91 6.09 0.40 45.56 4.13 0.39 46.52 4.90 0.43 47.46 6.07 0.42 39.09 4.40 0.36 50.16 5.30 0.45 45.14 5.56 0.34 45.16 4.86
0.39 46.24 5.10 0.04 3.19 0.70
0.34 49.66 4.72 0.38 47.44 4.51 0.36 48.42 5.02 0.35 47.08 4.68 0.31 41.68 4.28 0.37 43.23 4.76 0.34 50.44 4.99 0.33 43.72 4.91 0.30 48.03 5.01
0.34 46.64 4.76 0.03 3.05 0.25
0.38 41.41 5.09 0.34 41.11 5.44 0.39 46.60 5.55 0.36 43.49 4.47 0.33 39.86 4.93 0.34 44.30 4.59 0.29 43.56 4.08 0.32 42.57 5.18 0.37 48.56 4.74
0.35 43.49 4.90 0.03 2.74 0.48
C18:2
0.23 0.27 0.27 0.35 0.30 0.32 0.34 0.31 0.31
0.30 0.04
0.23 0.32 0.30 0.26 0.30 0.38 0.28 0.28 0.37
0.30 0.05
0.30 0.28 0.33 0.32 0.30 0.32 0.31 0.27 0.35
0.31 0.02
0.27 0.28 0.35 0.30 0.32 0.36 0.29 0.26 0.32
0.31 0.03
0.33 0.29 0.24 0.26 0.28 0.28 0.27 0.29 0.34
0.29 0.03
0.30 0.27 0.30 0.31 0.29 0.26 0.30 0.34 0.26
0.29 0.03
0.40 0.52 0.48 0.32 0.41 0.32 0.25 0.41 0.34
0.38 0.09
C18:3
0.50 0.12 0.55 0.55 0.48 0.64 0.92 0.54 0.65
0.55 0.21
0.75 0.69 0.51 0.59 0.72 0.45 0.65 0.68 0.56
0.62 0.10
0.54 0.66 0.72 0.52 0.57 0.48 0.58 0.53 0.64
0.58 0.08
0.62 0.58 0.64 0.61 0.54 0.47 0.59 0.68 0.69
0.60 0.07
0.58 0.73 0.50 0.55 0.60 0.42 0.71 0.53 0.51
0.57 0.10
0.66 0.59 0.63 0.52 0.45 0.48 0.65 0.45 0.64
0.56 0.09
0.40 0.42 0.52 0.42 0.43 0.48 0.43 0.45 0.58
0.46 0.06
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Appendix to TNO-report of 3M-02
Final version
04-12-2006
fatty acid
total fatty acids C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
fatty acid
total fatty acids C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
fatty acid
total fatty acids C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
group 1 vs 2 nn p 9 9 0.258 9 9 0.297 9 9 0.136 9 9 0.666 9 9 0.191 9 9 0.222 9 9 0.931 9 9 0.190 group 2 vs 4 nn p 9 9 0.489 9 9 0.546 9 9 1.000 9 9 0.019 9 9 0.489 9 9 0.190 9 9 0.863 9 9 0.546 group 3 vs 7 nn p 9 9 <0.001 9 9 0.040 9 9 0.011 9 9 0.019 9 9 0.006 9 9 0.546 9 9 0.031 9 9 0.002
group 1 vs 3 nn p 9 9 0.730 9 9 0.436 9 9 0.340 9 9 0.730 9 9 0.297 9 9 0.863 9 9 0.666 9 9 0.666 group 2 vs 5 nn p 9 9 1.000 9 9 0.605 9 9 0.436 9 9 0.730 9 9 0.340 9 9 0.730 9 9 0.489 9 9 0.297 group 4 vs 5 nn p 9 9 1.000 9 9 0.113 9 9 0.161 9 9 0.019 9 9 0.094 9 9 0.387 9 9 0.297 9 9 0.340
fatty acid excretion, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
nn p nn p nn
p
9 9 0.040 9 9 0.387 9 9 0.546
9 9 0.094 9 9 0.436 9 9 0.190
9 9 0.050 9 9 0.436 9 9 0.489
9 9 0.136 9 9 0.605 9 9 0.077
9 9 0.050 9 9 0.546 9 9 0.546
9 9 0.931 9 9 0.387 9 9 0.863
9 9 0.863 9 9 0.387 9 9 0.387
9 9 0.387 9 9 0.863 9 9 1.000
group 2 vs 6 group 2 vs 7
group 3 vs 4
nn p nn p nn
p
9 9 0.222 9 9 0.008 9 9 0.040
9 9 0.931 9 9 0.063 9 9 0.222
9 9 0.222 9 9 0.008 9 9 0.190
9 9 0.014 9 9 0.040 9 9 0.014
9 9 0.546 9 9 0.008 9 9 0.077
9 9 0.094 9 9 0.436 9 9 1.000
9 9 0.796 9 9 0.024 9 9 0.730
9 9 0.190 9 9 0.001 9 9 0.436
group 4 vs 6 group 4 vs 7
group 5 vs 6
nn p nn p nn
p
9 9 0.040 9 9 <0.001 9 9 0.222
9 9 0.730 9 9 0.011 9 9 0.340
9 9 0.161 9 9 0.002 9 9 1.000
9 9 0.730 9 9 1.000 9 9 0.011
9 9 0.222 9 9 0.001 9 9 0.863
9 9 0.931 9 9 0.605 9 9 0.489
9 9 0.436 9 9 0.040 9 9 0.546
9 9 0.489 9 9 <0.001 9 9 1.000
group 1 vs 7 nn p 9 9 <0.001 9 9 0.222 9 9 0.136 9 9 0.136 9 9 0.136 9 9 0.605 9 9 0.019 9 9 0.031 group 3 vs 5 nn p 9 9 0.297 9 9 0.931 9 9 0.931 9 9 0.863 9 9 1.000 9 9 0.340 9 9 0.136 9 9 0.796 group 5 vs 7 nn p 9 9 0.004 9 9 0.136 9 9 0.050 9 9 0.019 9 9 0.063 9 9 0.605 9 9 0.011 9 9 0.014
group 2 vs 3 nn p 9 9 0.297 9 9 0.730 9 9 0.387 9 9 0.730 9 9 0.387 9 9 0.113 9 9 0.546 9 9 0.340 group 3 vs 6 nn p 9 9 0.730 9 9 0.666 9 9 0.796 9 9 0.008 9 9 1.000 9 9 0.863 9 9 0.161 9 9 0.666 group 6 vs 7 nn p 9 9 0.002 9 9 0.040 9 9 0.077 9 9 0.796 9 9 0.050 9 9 0.489 9 9 0.008 9 9 0.008
p<0.05
Page A119 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Fatty acid balance
group
1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 AVERAGE SD
cage#
1,2 3,4 5 1,2 3,4 5 1,2 3,4 5
GC sample#
1:1-1 1:1-2 1:1-3 2:1-1 2:1-2 2:1-3 3:1-1 3:1-2 3:1-3
6
1:2-4
7,8
1:2-5
9,10
1:2-6
6
2:2-4
7,8
2:2-5
9,10
2:2-6
6
3:2-4
7,8
3:2-5
9,10
3:2-6
11,12,13 15
14,16,17 11,12,13
15 14,16,17 11,12,13
15 14,16,17
1:3-7 1:3-8 1:3-9 2:3-7 2:3-8 2:3-9 3:3-7 3:3-8 3:3-9
18 19 20,21 18 19 20,21 18 19 20,21
1:4-10 1:4-11 1:4-12 2:4-10 2:4-11 2:4-12 3:4-10 3:4-11 3:4-12
22 24,25 23,26,27
22 24,25 23,26,27
22 24,25 23,26,27
1:5-13 1:5-14 1:5-15 2:5-13 2:5-14 2:5-15 3:5-13 3:5-14 3:5-15
28,29 31
30,32 28,29
31 30,32 28,29
31 30,32
1:6-16 1:6-17 1:6-18 2:6-16 2:6-17 2:6-18 3:6-16 3:6-17 3:6-18
33,34,35 36,37 38,39
33,34,35 36,37 38,39
33,34,35 36,37 38,39
1:7-19 1:7-20 1:7-21 2:7-19 2:7-20 2:7-21 3:7-19 3:7-20 3:7-21
total fatty acids (mol/g feces)
241.33 292.24 345.18 208.40 246.11 326.56 304.96 310.59 290.07
454.92 546.64 155.01 364.58 450.12 112.97 372.45 428.65 153.80
183.38 284.77 334.26 175.80 308.29 243.12 160.36 221.76 351.76
367.06 238.78 351.76 353.44 296.84 289.63 305.94 351.28 410.51
291.81 499.96 194.27 327.35 460.38 113.53 373.01 452.42 126.73
317.21 243.23 260.59 266.16 142.20 194.69 340.17 179.90 287.68
96.09 93.92 154.30 137.09 95.99 144.75 163.94 106.31 168.41
total feces (g)
3.075 2.4897 2.2946 4.692 3.7789 3.4106 3.5754 2.8781 2.7589
2.7619 4.0581 2.0935 4.075 6.371 3.4074 2.9279 4.3985 2.4575
3.4597 2.2994 2.3454 5.8147 3.7718 3.7727 4.025 2.5265 2.6905
1.689 3.1432 3.2545 2.7133 4.6407 5.2075 2.041 3.4464 3.9549
3.195 2.9408 2.6399 4.9712 4.4833 3.767 3.6113 3.3522 2.7034
2.5036 2.7083 2.3554 3.9684 3.9678 3.5666 2.9076 3.2449 2.7643
1.9697 2.0798 2.6705 3.4477 3.2646 4.3059 2.5117 2.4369 3.4107
average weight days
(g)
123.6
2
87.25
2
88.55
2
122.45
3
86.55
3
88.2
3
121.65
2
86.55
2
88.05
2
94.55
2
126.6
2
82.2
2
94.25
3
126.45
3
82.1
3
94
2
126.6
2
81.85
2
118
2
83.55
2
82.8
2
118.75
3
83.5
3
83.05
3
119.6
2
84.2
2
83.45
2
59.5
2
116.4
2
112.2
2
58.85
3
116.55
3
111.85
3
58.7
2
117.45
2
112.9
2
110.7
2
88.35
2
84.6
2
111.6
3
88.5
3
85.55
3
112.85
2
88.9
2
85.5
2
87.1
2
112.8
2
85.4
2
87.15
3
112.45
3
84.55
3
87.65
2
111.95
2
84.65
2
81.35
2
82.25
2
101.05
2
82.35
3
82.75
3
101.95
3
83.5
2
83.35
2
103.2
2
fatty acids excretion (mol/100 gr mouse/day)
300.20 416.95 447.24 266.18 358.19 420.92 448.15 516.42 454.44
403.19 80.09
664.43 876.12 197.40 525.43 755.96 156.29 580.05 744.63 230.89
525.69 268.58
268.83 391.86 473.41 286.94 464.20 368.14 269.83 332.70 567.05
380.33 103.97
520.97 322.39 510.16 543.18 393.97 449.48 531.88 515.39 719.00
500.71 110.09
421.11 832.08 303.10 486.06 777.41 166.63 596.83 852.97 200.35
515.17 265.81
455.90 292.00 359.37 403.99 167.25 273.76 564.22 260.72 469.72
360.77 124.47
116.33 118.75 203.89 191.32 126.23 203.79 246.56 155.41 278.30
182.29 57.68
food intake (g) 23.2 18 16.8 36.8 28.8 25.6 20.2 15.3 13.8
19.9 27.7 16.3 28.8 45.4 28.2 16.7 23.6 13.2
26.2 16.5 18.3 44.7 28.3 29.3 23.7 13.6 14.7
12 21.7 24 19.8 35.2 38.2 11.3 18.7 22.6
21.5 19.6 19.5 36.5 32.2 30.3 18.7 28 14.5
17.6 20.3 17.2 29.7 31 27.9 16.5 17.1 15
16.8 16.9 21.9 29.2 26.5 35 15.7 15 19.8
fatty acids uptake (mol/100 gr mouse/day)
5283.22 5806.79 5340.11 5639.32 6244.01 5446.40 4673.78 4975.69 4411.43
5924.08 6158.51 5581.42 5733.89 6737.13 6445.31 5000.55 5246.96 4539.25
6249.55 5558.62 6220.85 7063.37 6359.72 6620.12 5577.59 4546.28 4958.16
5676.67 5247.30 6020.71 6313.30 5667.20 6408.63 5418.38 4481.44 5634.35
5466.64 6244.23 6487.74 6137.15 6827.33 6646.00 4664.12 8865.14 4773.44
5687.53 5065.43 5668.91 6394.80 5172.97 6191.97 5298.60 4299.33 4987.62
5812.74 5783.36 6100.10 6653.61 6009.19 6441.97 5292.28 5065.41 5400.26
cholesterol balance (mol/100 gr mouse/day)
4983.02 5389.84 4892.87 5373.15 5885.82 5025.48 4225.64 4459.27 3956.98
4910.23 611.93
5259.65 5282.39 5384.03 5208.45 5981.17 6289.03 4420.50 4502.33 4308.36
5181.77 682.54
5980.71 5166.76 5747.45 6776.43 5895.52 6251.98 5307.76 4213.57 4391.11
5525.70 841.88
5155.70 4924.91 5510.55 5770.13 5273.22 5959.15 4886.51 3966.05 4915.34
5151.28 587.51
5045.52 5412.15 6184.64 5651.09 6049.92 6479.38 4067.29 8012.16 4573.08
5719.47 1159.56
5231.63 4773.43 5309.54 5990.81 5005.72 5918.21 4734.38 4038.62 4517.90
5057.81 634.70
5696.42 5664.61 5896.21 6462.29 5882.96 6238.18 5045.71 4910.00 5121.96
5657.59 537.83
molw
C14:0 C16:0 C16:1 C18:0 C18:1 C18:2 C18:3
average
228.4 256.4 254.4 284.5 282.5 280.5 278.5
266.46
MYSTRIC ACID PALMITIC ACID PALMITOLEIC ACID STEARIC ACID OLEIC ACID LINOLEIC ACID LINOLENIC ACID
g/mol
diet: 15 % fat=
0.563
mmol per g diet
Page A120 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
fatty acid balance balance
fatty acid balance balance
fatty acid balance balance
group 1 vs 2 nn p 9 9 0.436 group 2 vs 4 nn p 9 9 0.863 group 3 vs 7 nn p 9 9 1.000
group 1 vs 3 nn p 9 9 0.113 group 2 vs 5 nn p 9 9 0.258 group 4 vs 5 nn p 9 9 0.222
fatty acid balance, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
nn p nn p nn
p
9 9 0.489 9 9 0.063 9 9 0.730
group 2 vs 6 group 2 vs 7
group 3 vs 4
nn p nn p nn
p
9 9 0.796 9 9 0.258 9 9 0.222
group 4 vs 6 group 4 vs 7
group 5 vs 6
nn p nn p nn
p
9 9 0.730 9 9 0.136 9 9 0.136
group 1 vs 7 nn p 9 9 0.019 group 3 vs 5 nn p 9 9 0.863 group 5 vs 7 nn p 9 9 0.931
group 2 vs 3 nn p 9 9 0.546 group 3 vs 6 nn p 9 9 0.297 group 6 vs 7 nn p 9 9 0.094
p<0.05
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Appendix to TNO-report of 3M-02
Final version
Appendix F. Intestinal cholesterol absorption
Cholesterol absorption
Oral gavage mix:
14 C dpm average:
76083.3 76547.5 75205.8 74174.5 73763.5 75669.7 75241
3H dpm average:
92065.2 92196.7 90472.4 88575.4 92526.8 92548.9 91398
14C/3H dosing:
0.823
Mouse# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
14C dpm feces 7900.00 9070.58 8759.79
7943.46 9442.29 9027.49
9996.01 10914.90 11495.20
8402.11 8966.49 9560.47
10754.70 11858.50 12382.30
8918.47 9795.44 9570.76
11342.40 10247.10 11420.10
10160.50 11533.30 11375.70
9360.62 9242.62 9409.35
10257.10 11572.10 11790.60
7215.69 6854.01 8420.46
7620.85 7592.86 7701.77
10718.20 9765.43 11045.10
8973.56 10118.90 10186.80
11415.30 11521.70 9926.39
9713.21 10522.10 10357.80
10622.10 10309.40 9363.58
9289.29 8492.80 9169.78
7720.92 8438.85 8631.31
10252.90 9662.34 10589.00
3H dpm feces 17857.0 20224.1 19669.7
AVERAGE 17369.9 20044.0 19314.7
AVERAGE 16226.5 17696.9 19154.0
AVERAGE 15319.6 16793.3 16635.3
AVERAGE 19111.4 20818.2 21352.2
AVERAGE 19316.9 21221.6 20766.7
AVERAGE 19935.8 17921.2 19737.7
AVERAGE 22122.3 24951.5 24849.9
AVERAGE 18676.0 18523.6 18338.6
AVERAGE 18752.4 20953.2 21592.7
AVERAGE 13209.8 12708.1 14817.4
AVERAGE 13562.2 13608.7 13685.9
AVERAGE 22219.9 20159.2 22642.2
AVERAGE 15531.3 17347.9 17603.2
AVERAGE 21059.0 21372.1 19181.1
AVERAGE 17538.7 18995.2 18714.0
AVERAGE 17065.5 16759.2 15186.6
AVERAGE 18158.2 16878.2 18096.8
AVERAGE 15528.4 17238.0 17527.6
AVERAGE 18655.8 17086.2 19019.0
AVERAGE
14/3H feces 0.442 0.449 0.445 0.445 0.457 0.471 0.467 0.465 0.616 0.617 0.600 0.611 0.548 0.534 0.575 0.552 0.563 0.570 0.580 0.571 0.462 0.462 0.461 0.461 0.569 0.572 0.579 0.573 0.459 0.462 0.458 0.460 0.501 0.499 0.513 0.504 0.547 0.552 0.546 0.548 0.546 0.539 0.568 0.551 0.562 0.558 0.563 0.561 0.482 0.484 0.488 0.485 0.578 0.583 0.579 0.580 0.542 0.539 0.518 0.533 0.554 0.554 0.553 0.554 0.622 0.615 0.617 0.618 0.512 0.503 0.507 0.507 0.497 0.490 0.492 0.493 0.550 0.566 0.557 0.557
% cholesterol absorption 45.89 43.48 25.78 32.90 30.67 43.95 30.38 44.15 38.73 33.38 33.03 31.87 41.10 29.56 35.27 32.73 24.92 38.39 40.11 32.30
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Mouse# 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45
14C dpm feces 8632.76 7757.78 8732.12
9317.58 10349.10 10995.40
12726.80 11922.70 10824.40
11803.80 11509.60 10446.20
9824.33 10536.10 10819.00
9944.17 9896.95 9024.08
11012.10 12345.10 12611.60
10539.20 11997.90 11733.20
8467.17 8615.23 7791.76
10611.20 11669.60 11704.20
6797.41 8479.36 8042.25
9667.22 10332.00 10290.40
9274.67 9043.46 8359.95
8337.87 8978.72 9290.98
9260.73 8926.69 8216.57
7388.65 7801.01 8272.11
8775.47 8627.50 7935.44
8265.72 7672.71 7372.15
6512.96 7565.00 7444.81
8569.40 9540.76 9523.59
8330.02 9412.73 9427.18
9607.51 9889.25 9677.26
10990.90 9651.99 11648.90
9120.82 10887.20 11023.60
7559.63 8832.90 9369.62
3H dpm feces 18263.4 16543.7 18151.9
AVERAGE 18797.2 20750.2 21398.2
AVERAGE 21922.9 20859.7 19073.0
AVERAGE 20531.4 19930.8 17926.4
AVERAGE 17823.8 19638.0 19717.7
AVERAGE 17861.6 17830.0 16251.3
AVERAGE 20530.5 22826.4 23740.9
AVERAGE 19138.3 21840.1 21335.4
AVERAGE 19713.7 19837.0 18343.0
AVERAGE 16118.3 17565.2 17995.9
AVERAGE 15090.2 18363.5 17655.2
AVERAGE 18299.0 19525.1 19416.7
AVERAGE 18965.3 18555.8 17064.5
AVERAGE 16938.5 18142.5 18500.1
AVERAGE 21618.5 20655.8 19301.2
AVERAGE 17101.3 18082.3 19128.3
AVERAGE 18331.8 18170.1 16944.8
AVERAGE 17166.9 16099.9 15533.3
AVERAGE 13424.4 15510.5 15298.9
AVERAGE 17514.8 19514.6 19814.4
AVERAGE 16006.8 17737.5 17550.5
AVERAGE 19624.7 19941.0 19580.0
AVERAGE 20009.6 17422.8 20445.0
AVERAGE 17747.4 20878.9 21294.1
AVERAGE 14314.6 16604.8 17203.4
AVERAGE
14/3H feces 0.473 0.469 0.481 0.474 0.496 0.499 0.514 0.503 0.581 0.572 0.568 0.573 0.575 0.577 0.583 0.578 0.551 0.537 0.549 0.545 0.557 0.555 0.555 0.556 0.536 0.541 0.531 0.536 0.551 0.549 0.550 0.550 0.430 0.434 0.425 0.430 0.658 0.664 0.650 0.658 0.450 0.462 0.456 0.456 0.528 0.529 0.530 0.529 0.489 0.487 0.490 0.489 0.492 0.495 0.502 0.496 0.428 0.432 0.426 0.429 0.432 0.431 0.432 0.432 0.479 0.475 0.468 0.474 0.481 0.477 0.475 0.478 0.485 0.488 0.487 0.487 0.489 0.489 0.481 0.486 0.520 0.531 0.537 0.529 0.490 0.496 0.494 0.493 0.549 0.554 0.570 0.558 0.514 0.521 0.518 0.518 0.528 0.532 0.545 0.535
% cholesterol absorption 42.39 38.93 30.37 29.74 33.74 32.50 34.87 33.19 47.82 20.11 44.62 35.72 40.63 39.69 47.92 47.53 42.43 41.99 40.90 40.93 35.69 40.08 32.26 37.12 35.02
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Mouse# 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70
14C dpm feces 13292.00 12025.90 8154.44
7073.99 8303.55 8312.14
8105.89 9177.91 8404.42
9083.21 10025.30 10551.50
7998.17 9589.24 9485.67
8469.79 9318.33 9497.10
8613.92 8699.27 7646.35
10299.70 9010.87 10286.90
8794.09 9327.04 9893.72
12083.00 11744.30 10287.20
7269.37 8544.20 8283.26
7909.58 7041.53 8328.59
7120.23 8146.99 8204.53
8975.84 9015.56 7523.90
7587.01 8719.17 8811.04
7582.08 8218.83 8867.68
8808.83 8584.87 7447.43
7758.45 6896.60 7801.31
8952.17 10058.20 10221.10
8171.80 7457.41 8538.29
8381.63 7890.69 7086.95
9800.20 11238.90 11334.00
6081.05 6823.98 6967.67
7436.52 8308.62 8710.60
8595.56 8773.69 7650.67
3H dpm feces 23039.1 20891.1 14093.8
AVERAGE 11977.8 13879.6 13866.1
AVERAGE 14355.8 16142.2 14482.0
AVERAGE 16293.9 17848.5 18335.8
AVERAGE 17522.8 20188.8 20422.8
AVERAGE 18261.6 19807.5 20101.5
AVERAGE 19823.8 19531.5 17499.6
AVERAGE 20630.3 17880.3 20622.3
AVERAGE 18229.9 19419.7 20500.1
AVERAGE 22066.1 21734.5 19073.9
AVERAGE 16991.1 19484.0 19099.1
AVERAGE 18224.2 16492.5 19376.2
AVERAGE 17677.8 19926.7 19132.5
AVERAGE 20407.6 19983.1 17563.4
AVERAGE 17288.6 19191.4 19513.2
AVERAGE 18143.7 20142.1 21451.9
AVERAGE 21295.7 20799.9 18297.0
AVERAGE 17723.8 15907.7 18207.4
AVERAGE 21000.2 23550.7 23744.5
AVERAGE 18430.1 17029.8 19260.2
AVERAGE 20381.9 19487.6 17369.0
AVERAGE 23428.2 26990.4 26923.6
AVERAGE 14094.2 15200.4 16000.0
AVERAGE 18241.1 20465.5 21554.3
AVERAGE 21140.4 21390.2 18677.5
AVERAGE
14/3H feces 0.577 0.576 0.579 0.577 0.591 0.598 0.599 0.596 0.565 0.569 0.580 0.571 0.557 0.562 0.575 0.565 0.456 0.475 0.464 0.465 0.464 0.470 0.472 0.469 0.435 0.445 0.437 0.439 0.499 0.504 0.499 0.501 0.482 0.480 0.483 0.482 0.548 0.540 0.539 0.542 0.428 0.439 0.434 0.433 0.434 0.427 0.430 0.430 0.403 0.409 0.429 0.413 0.440 0.451 0.428 0.440 0.439 0.454 0.452 0.448 0.418 0.408 0.413 0.413 0.414 0.413 0.407 0.411 0.438 0.434 0.428 0.433 0.426 0.427 0.430 0.428 0.443 0.438 0.443 0.442 0.411 0.405 0.408 0.408 0.418 0.416 0.421 0.419 0.431 0.449 0.435 0.439 0.408 0.406 0.404 0.406 0.407 0.410 0.410 0.409
% cholesterol absorption 29.90 27.59 30.62 31.38 43.48 43.04 46.68 39.18 41.48 34.11 47.36 47.73 49.77 46.58 45.55 49.82 50.06 47.37 48.02 46.36 50.43 49.16 46.72 50.69 50.34
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Group# 1 1 1 1 1 1 1 1 1 1
AVERAGE SD 2 2 2 2 2 2 2 2 2 2
AVERAGE SD 3 3 3 3 3 3 3 3 3 3
AVERAGE SD 4 4 4 4 4 4 4 4 4 4
AVERAGE SD 5 5 5 5 5 5 5 5 5 5
AVERAGE SD 6 6 6 6 6 6 6 6 6 6
AVERAGE SD 7 7 7 7 7 7 7 7 7 7
AVERAGE SD
Mouse# 1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
% cholesterol absorption 45.89 43.48 25.78 32.90 30.67 43.95 30.38 44.15 38.73 33.38 36.9 7.2 33.03 31.87 41.10 29.56 35.27 32.73 24.92 38.39 40.11 32.30 33.9 5.0 42.39 38.93 30.37 29.74 33.74 32.50 34.87 33.19 47.82 20.11 34.4 7.5 44.62 35.72 40.63 39.69 47.92 47.53 42.43 41.99 40.90 40.93 42.2 3.7 35.69 40.08 32.26 37.12 35.02 29.90 27.59 30.62 31.38 43.48 34.3 4.9 43.04 46.68 39.18 41.48 34.11 47.36 47.73 49.77 46.58 45.55 44.1 4.7 49.82 50.06 47.37 48.02 46.36 50.43 49.16 46.72 50.69 50.34 48.9 1.6
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week 8/9 week 8/9 week 8/9
group 1 vs 2 nn p 10 10 0.315 group 2 vs 4 nn p 10 10 <0.001 group 3 vs 7 nn p 10 10 <0.001
Intestinal cholesterol absorption, p values Mann-Whitney U-test
group 1 vs 3 group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn p nn p nn p nn
p
nn p
10 10 0.529 10 10 0.143 10 10 0.353 10 10 0.015 10 10 <0.001
group 2 vs 5 group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
nn p nn p nn p nn
p
nn p
10 10 0.912 10 10 <0.001 10 10 <0.001 10 10 0.009 10 10 1.000
group 4 vs 5 group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn p nn p nn p nn
p
nn p
10 10 0.002 10 10 0.315 10 10 <0.001 10 10 0.001 10 10 <0.001
group 2 vs 3 nn p 10 10 0.796 group 3 vs 6 nn p 10 10 0.005 group 6 vs 7 nn p 10 10 0.003
p<0.05
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Appendix G. Liver weight and perigonadal fat weight and lipid contents liver
G1. Liver weight and perigonadal fat weight
group
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
Mouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
eartag LR
01 12 00 20 00 02 11 00 02 12
bodyweight (g)
34.5 31.9 30.4 28.9 32.4 30.1 28.4 32.8 32 28
1 0 35.2
2 1 30.3
02
34
0 1 34.9
1 0 30.4
0 1 31.3
2 0 32.9
0 0 29.2
2 1 28.8
1 0 31.2
01
31
02
31
0 1 33.6
1 1 29.8
1 0 27.7
0 1 30.9
2 0 31.2
1 2 26.3
0 2 32.3
1 0 29.6
1 0 28.3 0 2 28.5 0 0 29.1 1 0 29.8 0 1 31.1 0 2 26.4 0 0 27.6 1 1 30.1 0 0 27.8 1 1 28.3
1 0 31.8 0 1 25.9 1 1 29.6 1 2 29.2 1 2 29.6 2 0 30.2 0 1 31.9 1 1 30.1 0 0 29.2 1 2 29.6
01
28
1 0 31.2
1 2 25.9
1 0 27.5
0 2 26.9
0 0 25.4
0 1 26.6
0 2 27.8
22
30
2 1 26.4
2 0 29.9
10
30
0 2 28.7
0 0 30.3
1 2 29.4
0 0 28.3
0 0 28.1
20
28
10
25
01
28
perigonadal fat weight (g)
1.06 0.72 0.68 0.39 0.81 0.57 0.63 0.94 0.89 0.48
0.72 0.21
1.16 0.53 0.92 0.78 0.55 0.43 0.50 0.74 0.87 0.92
0.74 0.23
0.77 0.74 0.59 0.37 0.42 0.64 0.75 0.44 1.05 0.33
0.61 0.23
0.24 0.26 0.43 0.42 0.45 0.39 0.23 0.30 0.34 0.32
0.34 0.08
0.82 0.54 0.65 0.39 0.51 0.57 0.44 0.52 0.86 0.47
0.58 0.16
0.23 0.34 0.14 0.19 0.25 0.30 0.41 0.37 0.31 0.21
0.28 0.09
0.34 0.36 0.33 0.38 0.30 0.30 0.35 0.32 0.29 0.30
0.33 0.03
liver weight (g)
2.19 2
1.66 1.41 1.68 1.7 1.61 1.77 1.68 1.49
1.72 0.23
2.77 1.17 1.88 2.53 1.68 1.23 1.35 1.84 1.53 1.98
1.80 0.53
2 2.23 2.27 1.68 1.56 2.06 1.85 1.51 2.42 2.14
1.97 0.31
3.46 3.68 3.69 3.93 4.23 1.94 3.62 3.9 4.03 3.39
3.59 0.63
1.9 1.71 2.48 1.69 2.25 2.46 1.47 1.88 1.82 2.14
1.98 0.34
3.9 4.87 4.1 4.17 4.13 3.48 3.13 4.11 4.74 4.33
4.10 0.52
4.29 4.33 3.88 4.28 4.15 3.85 3.91 3.83 3.33 3.92
3.98 0.30
liver weight as % of bw (%)
6.35 6.27 5.46 4.88 5.19 5.65 5.67 5.40 5.25 5.32
5.54 0.46
7.87 3.86 5.53 7.25 5.53 3.93 4.10 6.30 5.31 6.35
5.60 1.38
6.45 7.19 6.76 5.64 5.63 6.67 5.93 5.74 7.49 7.23
6.47 0.71
12.23 12.91 12.68 13.19 13.60 7.35 13.12 12.96 14.50 11.98
12.45 1.92
5.97 6.60 8.38 5.79 7.60 8.15 4.61 6.25 6.23 7.23
6.68 1.16
13.93 15.61 15.83 15.16 15.35 13.70 11.77 14.78 15.80 16.40
14.83 1.37
14.35 14.43 13.52 14.13 14.12 13.60 13.91 13.68 13.32 14.00
13.91 0.37
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tissue
perigonadal fat liver
liver as % of bodyweight
tissue
perigonadal fat liver
liver as % of bodyweight
tissue
perigonadal fat liver
liver as % of bodyweight
Liverweight; perigonadal fat weight; liverweight as % of bodyweight, p values Mann-Whitney U-test
group 1 vs 2 group 1 vs 3 group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7 group 2 vs 3
nn p nn p nn p nn p nn
p
nn p nn p
10 10 0.912 10 10 0.315 10 10 <0.001 10 10 0.123 10 10 <0.001 10 10 <0.001 10 10 0.190
10 10 0.971 10 10 0.075 10 10 <0.001 10 10 0.043 10 10 <0.001 10 10 <0.001 10 10 0.247
10 10 0.796 10 10 0.003 10 10 <0.001 10 10 0.015 10 10 <0.001 10 10 <0.001 10 10 0.089
group 2 vs 4 group 2 vs 5 group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5 group 3 vs 6
nn p nn p nn p nn p nn
p
nn p nn p
10 10 <0.001 10 10 0.123 10 10 <0.001 10 10 <0.001 10 10 0.003 10 10 0.971 10 10 <0.001
10 10 <0.001 10 10 0.315 10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 0.912 10 10 <0.001
10 10 <0.001 10 10 0.105 10 10 <0.001 10 10 <0.001 10 10 <0.001 10 10 0.529 10 10 <0.001
group 3 vs 7 group 4 vs 5 group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7 group 6 vs 7
nn p nn p nn p nn p nn
p
nn p nn p
10 10 <0.001 10 10 <0.001 10 10 0.105 10 10 0.796 10 10 <0.001 10 10 <0.001 10 10 0.190
10 10 <0.001 10 10 <0.001 10 10 0.043 10 10 0.105 10 10 <0.001 10 10 <0.001 10 10 0.481
10 10 <0.001 10 10 <0.001 10 10 0.003 10 10 0.003 10 10 <0.001 10 10 <0.001 10 10 0.029
p<0.05
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Final version
G2. Lipid contents liver Liverhomogenates; protein concentration
mouse
1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
measured prot conc (mg/ml)
well 1
well 2
0.551
0.56
0.292
0.301
0.701
0.686
1.058
1.098
0.384
0.389
0.468
0.46
1.208
1.279
0.468
0.46
0.479
0.468
0.596
0.587
1.011
0.949
0.707
0.692
1.406
1.44
1.185
1.185
0.35
0.341
0.59
0.579
0.768
0.772
0.622
0.607
0.484
0.494
1.022
1.091
0.942
0.945
0.75
0.734
0.734
0.71
0.508
0.516
0.747
0.722
0.407
0.407
1.054
1.047
0.778
0.765
0.489
0.468
0.185
0.187
0.263
0.274
0.822
0.829
0.546
0.573
1.251
1.231
1.627
1.608
0.524
0.529
0.579
0.579
0.533
0.541
0.296
0.321
0.758
0.749
0.707
0.71
0.453
0.455
0.468
0.478
0.433
0.425
0.438
0.438
1.099
1.151
0.13
0.13
0.93
0.934
1.953
2.04
0.428
0.415
0.36
0.351
0.463
0.458
1.124
1.147
0.481
0.465
0.372
0.363
0.779
0.801
0.465
0.478
0.568
0.554
0.776
0.755
0.173
0.169
0.62
0.612
2.018
2.035
0.692
0.674
0.321
0.314
0.341
0.341
0.239
0.235
0.355
0.348
0.175
0.177
0.743
0.728
1.444
1.462
dilution
20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
Protein conc (mg/ml) 11.11 5.93 13.87 21.56 7.73 9.28 24.87 9.28 9.47 11.83 19.60 13.99 28.46 23.70 6.91 11.69 15.40 12.29 9.78 21.13 18.87 14.84 14.44 10.24 14.69 8.14 21.01 15.43 9.57 3.72 5.37 16.51 11.19 24.82 32.35 10.53 11.58 10.74 6.17 15.07 14.17 9.08 9.46 8.58 8.76 22.50 2.60 18.64 39.93 8.43 7.11 9.21 22.71 9.46 7.35 15.80 9.43 11.22 15.31 3.42 12.32 40.53 13.66 6.35 6.82 4.74 7.03 3.52 14.71 29.06
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HPTLC measurements, liver lipids:
mouse# 1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33
Peak#
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
Name
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Width [mm]
4.57 8.13 3.94 4.19 4.57 6.22 4.06 5.08 4.57 7.87 3.94 4.06
3.81 5.46 4.19 4.32 4.06 6.99 4.19 4.83 4.57 7.62 4.32 4.7 4.19 7.75 4.06 4.32 4.57 6.73 4.06 4.45 4.32 7.11 4.06 4.32 4.06 7.49 3.17 3.81
4.45 5.71 3.81 4.19 4.45 8.64 3.17 4.57 4.83
8 4.32 4.57
4.45 6.6 3.94 4.83 4.95 4.83 4.06 3.94 4.45 6.6 3.68 4.7 4.06 6.86 3.81 5.08 4.57 6.22 4.19 4.57
Pos [mm]
6.73 21.84 27.3 35.31 6.22 19.94 26.42 34.8 5.97 19.68 26.16 34.42
6.35 19.43 26.42 34.8 6.35 20.32 26.42 34.67 6.48 20.07 26.29 34.42 6.48 20.07 26.16 34.16 6.99 20.32 26.54 34.54 6.86 20.83 26.16 33.66 6.86 21.97 26.29 33.66
7.62 23.24 27.56 34.16 6.48 22.86 27.56 35.31 6.48 20.83 26.54 34.54
6.22 19.56 25.65 34.16 5.97 18.41 25.4 33.78 6.22 20.07 26.16 34.29 6.35 21.34 26.67 34.54 6.86 22.23 27.3 34.92
od-Bkg
62604 287988 22996 105220 52524 197880 24262 125607 43766 251729 26977 68880
34466 123478 27048 67378 52422 260322 24841 141768 50585 265253 24749 127056 48224 248371 28494 130184 44510 192428 25345 79085 54357 258846 23946 89672 57023 299353 22427 84768
39787 115041 21069 81470 51326 318787 21609 111253 41327 242241 25212 94035
46397 221527 27238 133113 35829 104710 25858 42916 44899 206251 22142 78487 51469 216844 25800 74388 46317 162768 26102 89651
g lipid/ 200 g protein
5.44
50.09
2.00
9.15
4.33
32.62
2.00
10.35
3.24
37.32
2.00
5.11
2.55
18.26
2.00
4.98
4.22
41.92
2.00
11.41
4.09
42.87
2.00
10.27
3.38
34.87
2.00
9.14
3.51
30.37
2.00
6.24
4.54
43.24
2.00
7.49
5.09
53.39
2.00
7.56
3.78
21.84
2.00
7.73
4.75
59.01
2.00
10.30
3.28
38.43
2.00
7.46
3.41
32.53
2.00
9.77
2.77
16.20
2.00
3.32
4.06
37.26
2.00
7.09
3.99
33.62
2.00
5.77
3.55
24.94
2.00
Page A130 of A172
6.87
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mouse# 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5
Peak#
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
Name
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Width [mm]
5.08 5.59 4.06 4.06 4.19 6.86 3.94 3.94 4.45 7.49 3.3 4.45
4.32 5.33 4.32 3.56 4.19 8.51 3.56 5.33 3.94 5.97 4.45 4.95 4.19 6.48 4.06 5.33 4.45 6.48 3.94 5.08 4.06 6.6 3.94 5.59 4.06 6.99 4.32 6.1
4.7 5.97 3.56 4.06 3.94 5.97 4.32 5.59 3.81 5.46 3.94 4.45
4.32 6.73 4.06 4.95 4.7 7.24 3.94 4.95 3.68 6.6 3.56 5.59 3.81 6.6 3.81 5.84 3.56 5.71 4.19 5.59
Pos [mm]
7.37 22.61 27.69 35.31 7.24 22.99 27.81 35.31 6.73 23.11 27.43 35.05
7.37 23.37 28.45 36.07 5.46 21.21 26.16 34.04 5.46 18.8 25.27 33.66 4.95 17.02
24 33.02 4.57
16 23.24 32.64
4.7 15.75 23.11 32.64 5.21
16 23.24 32.64
6.22 19.68 26.29 34.29 4.45 15.37 22.86 32.13 5.33 17.14 24.13 33.02
5.59 19.05 24.77 33.15 5.46 20.57 25.53 33.4 3.94 13.97 21.21 30.35 3.94 13.84 20.96 30.35 4.19 13.08 20.7 30.23
od-Bkg
39765 132487 27644 37392 49635 246286 23327 123642 60183 294481 22239 141459
38024 104943 29874 26558 35962 307860 24049 107934 40358 115325 25364 52857 42991 172245 24996 73646 56968 192630 26017 123337 56224 215795 27127 153105 55767 238689 31249 162067
53651 199383 25618 75588 38266 151763 32037 54371 49570 149995 26617 46177
61124 219127 28657 145012 56257 295337 28462 97524 48925 249297 28191 157664 65477 276227 27578 178277 49411 189703 29682 95600
g lipid/ 200 g protein 2.88 19.17 2.00 2.71 4.26 42.23 2.00 10.60 5.41 52.97 2.00 12.72 2.55 14.05 2.00 1.78 2.99 51.20 2.00 8.98 3.18 18.19 2.00 4.17 3.44 27.56 2.00 5.89 4.38 29.62 2.00 9.48 4.15 31.82 2.00 11.29 3.57 30.55 2.00 10.37 4.19 31.13 2.00 5.90 2.39 18.95 2.00 3.39 3.72 22.54 2.00 3.47 4.27 30.59 2.00 10.12 3.95 41.51 2.00 6.85 3.47 35.37 2.00 11.19 4.75 40.07 2.00 12.93 3.33 25.57 2.00 6.44
Page A131 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mouse# 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48
Peak#
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
Name
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Width [mm]
3.3 5.71 4.19 5.33 3.43 7.49 3.81 5.59 3.94 6.35 3.81 5.46
3.56 5.71 3.81 4.95 3.94 6.6 3.68 5.33 4.19 7.37 3.68 5.46 4.45 5.97 4.19 4.7 4.7 6.35 4.19 3.68 4.83 6.99 4.7 4.57 4.32 6.22 4.06 4.45
3.94 6.6 3.81 4.06 4.19 5.71 3.68 4.06 4.7 7.49 3.43 5.08
3.81 5.59 3.81 4.06 3.94 5.84 3.94 4.45 4.32 5.71 3.81 4.45 4.06 5.59 3.81 4.45 4.32 6.6 3.17 4.7
Pos [mm]
3.94 12.57 20.45 30.1 3.94 13.84 21.08 30.73 4.32 14.48 22.1 31.5
4.19 14.73 22.48 31.62 4.57 16.13 23.37 32.26 5.33 18.16 24.51 33.02 5.33 18.92 25.27 33.27 5.59 20.32 25.91 33.27 5.84 21.21 27.69 35.69 5.71 20.45 26.92 34.92
5.97 20.07 26.42 34.54 5.97 19.3 25.65 33.78 6.48 20.45 25.65 33.66
6.99 20.45 25.91 33.66 7.11 21.21 25.91 33.53 6.48 20.83 25.53 32.89 7.37 22.23 26.8 33.91 7.75 23.88 27.43 34.16
od-Bkg
51117 186121 31056 108021 51578 314298 30069 134094 49352 209014 27111 117556
43993 173724 24940 61245 53573 223574 24814 161398 55464 270083 24336 153330 51342 176085 23750 76943 47717 148024 25114 60069 45004 193690 27970 60245 43432 150439 25551 98765
42568 194874 23418 72154 40720 177809 27101 69768 52458 292884 29963 160643
50307 162127 27192 70970 56774 225869 27244 91446 49756 171023 26319 87109 41083 144165 27516 61184 55902 285090 26432 119215
g lipid/ 200 g protein 3.29 23.97 2.00 6.96 3.43 41.81 2.00 8.92 3.64 30.84 2.00 8.67 3.53 27.86 2.00 4.91 4.32 36.04 2.00 13.01 4.56 44.39 2.00 12.60 4.32 29.66 2.00 6.48 3.80 23.58 2.00 4.78 3.22 27.70 2.00 4.31 3.40 23.55 2.00 7.73 3.64 33.29 2.00 6.16 3.01 26.24 2.00 5.15 3.50 39.10 2.00 10.72 3.70 23.85 2.00 5.22 4.17 33.16 2.00 6.71 3.78 25.99 2.00 6.62 2.99 20.96 2.00 4.45 4.23 43.14 2.00 9.02
Page A132 of A172
Appendix to TNO-report of 3M-02
Final version
mouse# 58 68 9 19 29 39 49 59 69 10 20 30 40 50 60 70
Peak#
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4 1 2 3 4 1 2 3 4
1 2 3 4
1 2 3 4 1 2 3 4
1 2 3 4
1 2 3 4 1 2 3 4
1 2 3 4
Name
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester Free cholesterol
Triglycerides Internal standard Cholesterolester
Free cholesterol Triglycerides
Internal standard Cholesterolester
Width [mm]
3.81 6.73 3.43 4.57 3.56 8.89 4.06 5.71 3.17 5.59 3.81 4.95
3.3 4.7 3.94 4.95 3.3 6.99 3.68 5.33 3.56 5.08 4.19 5.33
3.94 4.83 4.32 4.95 3.94 5.97 4.06 5.71 3.17 8.13 4.32 5.46
3.43 5.21 3.68 4.83
3.43 6.22 3.68 4.7 3.17 5.08 3.68 4.32
4.06 5.46 2.92 4.83
3.56 4.83 3.43 3.94 4.19 6.35 2.79 4.83
4.7 7.49 3.17 4.45
Pos [mm]
7.11 23.24 27.43 34.16 4.57 14.61 22.1 32.13 4.57 12.19 20.83 31.24
4.32 11.68 20.57 31.12 3.81 12.57 20.57 31.12 3.81 11.68 20.7 31.24
4.06 11.81 21.34 32.13 3.81 12.57 21.84 32.38
4.7 14.86 22.99 33.4
4.57 13.59 22.99 33.27
4.83 14.99 23.75 33.91 5.08 15.49 24.38 34.16
8.76 27.43 30.1 35.18
8.89 25.78 29.34 35.18
8 24.77 28.57 35.05
7.49 23.11 27.81 34.8
od-Bkg
50057 254148 23859 136982 59495 368098 29699 172395 47364 186870 29695 97139
44604 114977 29508 85494 55495 290613 29976 133502 54475 146150 30815 126727
63475 140542 33224 84388 64340 238585 30644 139703 56623 368599 30442 178078
37007 124055 26723 78711
47883 216520 26901 83366 34039 136553 27780 92480
54261 222500 24418 136191
36509 87808 28028 35295 58652 238639 21843 134627
58311 269150 31958 124736
g lipid/ 200 g protein
4.20 42.61 2.00 11.48 4.01 49.58 2.00 11.61 3.19 25.17 2.00 6.54
3.02 15.59 2.00 5.79 3.70 38.78 2.00 8.91 3.54 18.97 2.00 8.23
3.82 16.92 2.00 5.08 4.20 31.14 2.00 9.12 3.72 48.43 2.00 11.70
2.77 18.57 2.00 5.89
3.56 32.20 2.00 6.20 2.45 19.66 2.00 6.66
4.44 36.45 2.00 11.15
2.61 12.53 2.00 2.52 5.37 43.70 2.00 12.33
3.65 33.69 2.00 7.81
04-12-2006
Page A133 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group#
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
Mouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
Liver lipids (g/200 g protein)
Free cholesterol Triglycerides Cholesterolester
5.44
50.09
9.15
3.51
30.37
6.24
2.77
16.20
3.32
2.55
14.05
1.78
3.33
25.57
6.44
4.19
31.13
5.90
4.32
29.66
6.48
3.70
23.85
5.22
3.19
25.17
6.54
2.77
18.57
5.89
2.99
51.20
8.98
2.39
18.95
3.39
4.33
32.62
10.35
4.54
43.24
7.49
3.29
23.97
6.96
3.80
23.58
4.78
4.06
37.26
7.09
4.17
33.16
6.71
3.02
15.59
5.79
3.56
32.20
6.20
3.24
37.32
5.11
5.09
53.39
7.56
3.99
33.62
5.77
3.18
18.19
4.17
3.72
22.54
3.47
3.43
41.81
8.92
3.22
27.70
4.31
3.78
25.99
6.62
3.70
38.78
8.91
2.61
12.53
2.52
2.55
18.26
4.98
3.78
21.84
7.73
3.55
24.94
6.87
3.44
27.56
5.89
4.27
30.59
10.12
3.64
30.84
8.67
3.40
23.55
7.73
2.99
20.96
4.45
3.54
18.97
8.23
2.45
19.66
6.66
2.88
19.17
2.71
4.38
29.62
9.48
3.95
41.51
6.85
3.53
27.86
4.91
4.22
41.92
11.41
4.75
59.01
10.30
3.64
33.29
6.16
4.23
43.14
9.02
3.82
16.92
5.08
5.37
43.70
12.33
4.09
42.87
10.27
3.28
38.43
7.46
4.26
42.23
10.60
4.15
31.82
11.29
3.47
35.37
11.19
4.32
36.04
13.01
3.01
26.24
5.15
4.20
42.61
11.48
4.20
31.14
9.12
4.44
36.45
11.15
3.38
34.87
9.14
3.41
32.53
9.77
5.41
52.97
12.72
3.57
30.55
10.37
4.75
40.07
12.93
4.56
44.39
12.60
3.50
39.10
10.72
4.01
49.58
11.61
3.72
48.43
11.70
3.65
33.69
7.81
Liver lipids (g/mg protein)
Free cholesterol Triglycerides Cholesterolester
27.22
250.5
45.76
17.56
151.8
31.20
13.86
81.0
16.60
12.73
70.3
8.89
16.65
127.8
32.21
20.94
155.7
29.51
21.62
148.3
32.40
18.50
119.2
26.10
15.95
125.9
32.71
13.85
92.8
29.45
17.89
132.3
28.48
4.41
51.0
9.92
14.95
256.0
44.88
11.94
94.7
16.97
21.65
163.1
51.77
22.70
216.2
37.45
16.46
119.9
34.78
19.00
117.9
23.92
20.28
186.3
35.45
20.84
165.8
33.57
15.12
77.9
28.97
17.80
161.0
30.99
18.07
155.9
33.87
3.44
55.1
9.86
16.22
186.6
25.53
25.43
267.0
37.80
19.95
168.1
28.83
15.91
90.9
20.84
18.62
112.7
17.35
17.15
209.0
44.59
16.09
138.5
21.54
18.90
130.0
33.10
18.51
193.9
44.54
13.03
62.7
12.59
17.98
155.9
28.67
3.28
61.0
11.14
12.74
91.3
24.91
18.88
109.2
38.67
17.74
124.7
34.35
17.20
137.8
29.46
21.33
152.9
50.60
18.20
154.2
43.36
17.00
117.8
38.65
14.93
104.8
22.24
17.68
94.9
41.13
12.25
98.3
33.29
16.80
118.6
35.67
2.78
23.2
8.64
14.38
95.9
13.53
21.90
148.1
47.41
19.77
207.5
34.26
17.64
139.3
24.56
21.10
209.6
57.07
23.75
295.1
51.49
18.18
166.4
30.81
21.15
215.7
45.10
19.10
84.6
25.40
26.85
218.5
61.64
20.38
178.1
39.13
3.45
63.8
15.76
20.44
214.4
51.34
16.39
192.2
37.30
21.28
211.2
53.00
20.73
159.1
56.44
17.35
176.9
55.93
21.59
180.2
65.04
15.03
131.2
25.74
20.98
213.0
57.41
21.00
155.7
45.59
22.22
182.2
55.77
19.70
181.6
50.36
2.49
27.5
11.40
16.92
174.3
45.69
17.03
162.7
48.87
27.06
264.8
63.61
17.85
152.8
51.86
23.74
200.3
64.65
22.79
222.0
63.01
17.51
195.5
53.61
20.03
247.9
58.05
18.60
242.2
58.50
18.25
168.4
39.03
19.98
203.1
54.69
3.43
39.4
8.45
Page A134 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
lipid
free cholesterol triglyceride
cholesterolester
lipid
free cholesterol triglyceride
cholesterolester
lipid
free cholesterol triglyceride
cholesterolester
group 1 vs 2 nn p 10 10 0.739 10 10 0.247 10 10 0.218 group 2 vs 4 nn p 10 10 0.353 10 10 0.089 10 10 0.684 group 3 vs 7 nn p 10 10 0.247 10 10 0.075 10 10 <0.001
group 1 vs 3 nn p 10 10 0.796 10 10 0.353 10 10 0.912 group 2 vs 5 nn p 10 10 0.218 10 10 0.436 10 10 0.529 group 4 vs 5 nn p 10 10 0.023 10 10 0.035 10 10 0.579
lipid contents liver, p values Mann-Whitney U-test
group 1 vs 4 group 1 vs 5
group 1 vs 6
nn p nn p nn
p
10 10 0.853 10 10 0.105 10 10 0.218
10 10 0.631 10 10 0.105 10 10 0.004
10 10 0.105 10 10 0.190 10 10 0.002
group 2 vs 6 group 2 vs 7
group 3 vs 4
nn p nn p nn
p
10 10 0.315 10 10 0.353 10 10 0.529
10 10 0.280 10 10 0.052 10 10 0.143
10 10 0.004 10 10 <0.001 10 10 0.143
group 4 vs 6 group 4 vs 7
group 5 vs 6
nn p nn p nn
p
10 10 0.052 10 10 0.075 10 10 0.739
10 10 <0.001 10 10 <0.001 10 10 0.971
10 10 0.005 10 10 <0.001 10 10 0.105
group 1 vs 7 nn p 10 10 0.190 10 10 0.002 10 10 <0.001 group 3 vs 5 nn p 10 10 0.089 10 10 0.353 10 10 0.105 group 5 vs 7 nn p 10 10 0.579 10 10 0.315 10 10 0.015
group 2 vs 3 nn p 10 10 0.739 10 10 0.912 10 10 0.280 group 3 vs 6 nn p 10 10 0.089 10 10 0.247 10 10 0.001 group 6 vs 7 nn p 10 10 0.971 10 10 0.315 10 10 0.393
p<0.05
Page A135 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
H. HMG-CoA reductase and cholesterol-7-hydroxylase activity in liver microsomes and sterol27-hydroxylase activity in liver mitochondria
H1. HMG-CoA reductase and cholesterol-7-hydroxylase activity in liver microsomes
Protein concentration isolated microsomes
Microsomes for HMG CoA reductase determination
mouse
1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
measured prot conc (mg/ml)
well 1
well 2
0.828
0.866
0.725
0.739
1.052
1.102
1.42
1.525
0.927
0.977
0.659
0.654
0.654
0.688
0.763
0.801
1.019
1.073
0.788
0.818
1.667
1.803
1.031
1.056
0.64
0.647
0.691
0.709
0.789
0.828
0.669
0.688
0.718
0.739
0.876
0.888
0.664
0.677
1.86
1.983
1.733
1.851
0.583
0.604
0.752
0.773
0.718
0.729
1.124
1.161
0.734
0.752
1.555
1.696
1.756
1.961
0.7
0.723
0.519
0.521
0.822
0.846
1.223
1.308
1.136
1.176
1.649
1.791
0.651
0.643
0.622
0.628
0.98
1.041
0.98
0.991
0.763
0.796
0.881
0.91
1.149
1.216
1.2
1.281
0.66
0.734
0.597
0.64
1.127
1.198
1.254
1.315
0.67
0.699
1.697
1.712
1.641
1.823
0.637
0.668
0.794
0.826
0.832
0.859
1.63
1.553
0.796
0.817
1.747
1.899
0.636
0.632
1.097
1.149
0.736
0.766
0.92
0.906
1.503
1.47
0.98
1.034
0.621
0.612
1.154
1.219
0.798
0.82
0.807
0.794
1.17
1.203
1.685
1.612
0.629
0.657
1.092
1.048
1.529
1.598
dilution
20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 60 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 60 20 20 20 20 20 60 20 20 20 20 20 20 20 20
Protein conc (mg/ml) 16.94 14.64 21.54 29.45 19.04 39.39 40.26 15.64 20.92 16.06 34.70 20.87 38.61 42.00 16.17 13.57 14.57 17.64 13.41 38.43 35.84 11.87 15.25 14.47 22.85 14.86 32.51 37.17 14.23 10.40 16.68 25.31 23.12 34.40 38.82 12.50 20.21 19.71 15.59 17.91 23.65 24.81 13.94 12.37 23.25 25.69 13.69 34.09 34.64 13.05 16.20 16.91 31.83 16.13 36.46 38.04 22.46 15.02 18.26 29.73 20.14 36.99 23.73 16.18 16.01 23.73 32.97 12.86 21.40 31.27
Microsomes for 7-alpha-hydroxylase determination
mouse
1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
Code
1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 1.10 1.11 1.12 1.13 1.14 1.15 1.16 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 2.10 2.11 2.12 2.13 2.14 2.15 2.16 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 3.10 3.11 3.12 3.13 3.14 3.15 3.16 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4.10 4.11 4.12 4.13 4.14 4.15 4.16 5.1 5.2 5.3 5.4 5.5 5.6
measured prot conc (mg/ml)
well 1
well 2
0.952
0.996
0.683
0.703
1.097
1.048
1.543
1.438
1.222
1.218
0.652
0.644
0.612
0.694
1.103
1.113
1.366
1.329
0.999
0.963
1.524
1.447
0.921
0.909
0.569
0.575
0.577
0.571
0.708
0.679
0.553
0.545
0.918
0.874
1.15
1.14
0.69
0.694
0.5
0.436
0.543
0.479
0.599
0.569
0.887
0.837
0.726
0.724
0.882
0.893
0.772
0.829
0.541
0.515
0.61
0.579
0.604
0.624
0.488
0.498
0.674
0.67
1.263
1.329
1.023
1.055
0.507
0.472
0.505
0.492
0.595
0.581
0.808
0.79
0.994
0.988
0.673
0.646
1.164
1.183
0.472
0.488
0.385
0.376
0.792
0.772
0.561
0.592
0.942
0.933
1.404
1.527
0.933
0.948
0.462
0.479
0.35
0.366
0.74
0.699
0.818
0.821
0.837
0.859
1.604
1.483
0.829
0.824
0.45
0.46
0.468
0.455
0.821
0.865
0.832
0.826
0.916
0.954
1.164
1.132
0.893
0.951
0.455
0.468
0.432
0.41
0.821
0.845
0.994
1.039
1.268
1.517
0.483
0.525
0.779
0.785
0.919
0.87
0.553
0.511
dilution
20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 20 20 60 60 20 20 20 60 20 20 60
Protein conc (mg/ml) 19.48 13.86 21.45 29.81 24.40 38.88 39.18 22.16 26.95 19.62 29.71 18.30 34.32 34.44 13.87 10.98 17.92 22.90 13.84 28.08 30.66 11.68 17.24 14.50 17.75 16.01 31.68 35.67 12.28 9.86 13.44 25.92 20.78 29.37 29.91 11.76 15.98 19.82 13.19 23.47 28.80 22.83 15.64 11.53 18.75 29.31 18.81 28.23 21.48 14.39 16.39 16.96 30.87 16.53 27.30 27.69 16.86 16.58 18.70 22.96 18.44 27.69 25.26 16.66 20.33 27.85 30.24 15.64 17.89 31.92
Page A136 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Amount isolated liver microsomes
group# mouse#
1
1
1
2
1
3
1
4
1
5
1
6
1
7
1
8
1
9
1
10
AVERAGE
SD
2
11
2
12
2
13
2
14
2
15
2
16
2
17
2
18
2
19
2
20
AVERAGE
SD
3
21
3
22
3
23
3
24
3
25
3
26
3
27
3
28
3
29
3
30
AVERAGE
SD
4
31
4
32
4
33
4
34
4
35
4
36
4
37
4
38
4
39
4
40
AVERAGE
SD
5
41
5
42
5
43
5
44
5
45
5
46
5
47
5
48
5
49
5
50
AVERAGE
SD
6
51
6
52
6
53
6
54
6
55
6
56
6
57
6
58
6
59
6
60
AVERAGE
SD
7
61
7
62
7
63
7
64
7
65
7
66
7
67
7
68
7
69
7
70
AVERAGE
SD
Protein conc (mg/ml)
microsomes
microsomes
7 alpha hydroxylase HMGCo A reduct.
19.48
16.94
22.16
15.64
13.87
16.17
11.68
11.87
11.76
12.50
12.28
14.23
15.64
13.94
14.39
13.05
16.86
22.46
16.66
16.18
estimated l microsomes
600 600 600 600 600 600 600 600 600 600
mg microsomes 7 alpha hydroxylase
11.688 13.296 8.322 7.008 7.056 7.368 9.384 8.634 10.116 9.996
mg microsomes HMGCo A reduct.
10.164 9.384 9.702 7.122
7.5 8.538 8.364 7.83 13.476 9.708
17.24 9.86 13.86 26.95 15.98 11.53 10.98 16.39 16.58 20.33
15.25
600
10.40
600
14.64
600
20.92
600
20.21
600
12.37
600
13.57
600
16.20
600
15.02
600
16.01
600
10.344 5.916 8.316 16.17 9.588 6.918 6.588 9.834 9.948 12.198
9.15 6.24 8.784 12.552 12.126 7.422 8.142 9.72 9.012 9.606
21.45 19.62 17.92 14.5 13.44 19.82 18.75 16.96 18.7 15.64
21.54
600
16.06
600
14.57
600
14.47
600
16.68
600
19.71
600
23.25
600
16.91
600
18.26
600
12.86
600
12.87 11.772 10.752
8.7 8.064 11.892 11.25 10.176 11.22 9.384
12.924 9.636 8.742 8.682 10.008 11.826 13.95 10.146 10.956 7.716
29.81 29.71 22.9 17.75 25.92 13.19 29.31 30.87 22.96 27.85
29.45
700
34.70
700
17.64
700
22.85
700
25.31
700
15.59
600
25.69
700
31.83
700
29.73
700
23.73
700
20.867 20.797 16.03 12.425 18.144 7.914 20.517 21.609 16.072 19.495
20.615 24.29 12.348 15.995 17.717 9.354 17.983 22.281 20.811 16.611
13.84 16.01 20.78 23.47 24.4 18.3 18.81 16.53 18.44 17.89
13.41
600
14.86
600
23.12
600
17.91
600
19.04
600
20.87
600
13.69
600
16.13
600
20.14
600
21.40
600
8.304 9.606 12.468 14.082 14.64 10.98 11.286 9.918 11.064 10.734
8.046 8.916 13.872 10.746 11.424 12.522 8.214 9.678 12.084 12.84
38.88 34.32 28.08 31.68 29.37 28.8 28.23 27.3 27.69 30.24
39.39
700
38.61
700
38.43
700
32.51
700
34.40
700
23.65
700
34.09
700
36.46
700
36.99
700
32.97
700
27.216 24.024 19.656 22.176 20.559 20.16 19.761 19.11 19.383 21.168
27.573 27.027 26.901 22.757 24.08 16.555 23.863 25.522 25.893 23.079
39.18 34.44 30.66 35.67 29.91 22.83 21.48 27.69 25.26 31.92
40.26
700
42.00
700
35.84
700
37.17
700
38.82
700
24.81
700
34.64
700
38.04
700
23.73
700
31.27
700
27.426 24.108 21.462 24.969 20.937 15.981 15.036 19.383 17.682 22.344
28.182 29.4
25.088 26.019 27.174 17.367 24.248 26.628 16.611 21.889
isolated microsomes (mg)
21.85 22.68 18.02 14.13 14.56 15.91 17.75 16.46 23.59 19.70 18.47 3.37 19.49 12.16 17.10 28.72 21.71 14.34 14.73 19.55 18.96 21.80 18.86 4.72 25.79 21.41 19.49 17.38 18.07 23.72 25.20 20.32 22.18 17.10 21.07 3.15 41.48 45.09 28.38 28.42 35.86 17.27 38.50 43.89 36.88 36.11 35.19 8.44 16.35 18.52 26.34 24.83 26.06 23.50 19.50 19.60 23.15 23.57 22.14 3.42 54.79 51.05 46.56 44.93 44.64 36.72 43.62 44.63 45.28 44.25 45.65 4.74 55.61 53.51 46.55 50.99 48.11 33.35 39.28 46.01 34.29 44.23 45.19 7.58
used liver mg microsomes for isolation (g) per g liver
1.92
11.38
1.76
12.89
1.45
12.43
1.19
11.87
1.49
9.77
1.48
10.75
1.42
12.50
1.6
10.29
1.52
15.52
1.31
15.04
12.24
1.89
2.52
7.74
1.04
11.69
1.8
9.50
2.23
12.88
1.48
14.67
1.05
13.66
1.16
12.70
1.67
11.71
1.34
14.15
1.79
12.18
12.09
2.12
1.76
14.66
1.98
10.81
1.97
9.90
1.5
11.59
1.39
13.00
1.78
13.32
1.67
15.09
1.35
15.05
2.18
10.17
1.14
15.00
12.86
2.10
3.1
13.38
3.33
13.54
3.33
8.52
3.53
8.05
3.89
9.22
1.72
10.04
3.21
11.99
3.43
12.80
3.64
10.13
3.19
11.32
10.90
2.00
1.71
9.56
1.51
12.27
2.32
11.35
1.47
16.89
2.14
12.18
2.17
10.83
1.32
14.77
1.65
11.88
1.62
14.29
1.86
12.67
12.67
2.13
3.49
15.70
4.36
11.71
4.1
11.36
4.17
10.78
4.13
10.81
3.13
11.73
2.78
15.69
3.57
12.50
4.17
10.86
3.85
11.49
12.26
1.88
3.85
14.44
3.86
13.86
3.27
14.24
3.92
13.01
3.7
13.00
3.48
9.58
3.27
12.01
3.36
13.69
2.92
11.74
3.48
12.71
12.83
1.45
AVERAGE SD
group 1-7 12.26 1.97
Page A137 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Cholesterol-7--hydroxylase activity measurements in microsomes
used per assay 420 g protein 20 min incubation
internal standard 20 OH: 0.5 g per determination
Mouse 1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
7.489 7.764 8.344 7.459 7.921 8.048 7.859 8.072 8.614 8.284 8.279 7.708 7.956 8.246 8.569 8.034 8.103 8.551 8.177 7.462 8.615 8.176 7.997 8.678 7.153 8.014 7.760 7.119 8.211 8.341 7.684 7.654 8.062 8.155 8.529 8.412 8.674 8.281 9.215 7.851 8.260 8.138 9.023 9.148 8.783 7.856 7.946 7.525 7.842 7.885 8.094 7.994 7.866 7.808
area 7aOH
1.417 1.459 0.055 2.470 2.542 0.046 2.287 2.372 0.036 0.709 0.725 0.012 2.363 2.406 0.054 0.580 0.602 0.017 0.525 0.452 n.d. 2.048 2.039 0.036 0.912 0.988 0.018 1.032 1.302 0.026 0.389 0.369 n.d. 1.034 0.991 0.027 0.344 0.332 n.d. 0.414 0.408 n.d. 2.248 2.242 n.d. 2.554 2.519 0.055 2.020 2.034 0.031 0.878 0.863 n.d.
g 7aOH formed
0.095 0.094 0.003 0.166 0.160 0.003 0.146 0.147 0.002 0.043 0.044 0.001 0.149 0.146 0.003 0.036 0.037 0.001 0.032 0.030 0.000 0.125 0.127 0.002 0.064 0.062 0.001 0.072 0.079 0.002 0.025 0.024 0.000 0.063 0.058 0.002 0.020 0.020 0.000 0.026 0.025 0.000 0.125 0.123 0.000 0.163 0.159 0.004 0.129 0.129 0.002 0.055 0.055 0.000
7 alpha hydroxylase activity (nmol/h/mg protein) 1.681 2.959 2.662 0.785 2.661 0.658 0.576 2.296 1.137 1.373 0.456 1.093 0.368 0.472 2.282 2.898 2.346 1.014
Page A138 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
8.173 7.392 7.547 7.601 8.694 8.113 8.815 8.171 8.490 8.332 8.091 8.957 8.472 7.915 8.828 8.352 8.133 8.018 7.148 8.432 6.831 8.519 8.106 8.352 8.875 7.996 8.907 8.561 9.027 9.222 8.466 8.414 8.950 7.978 8.371 9.549 8.359 7.939 7.754 7.600 8.216 7.859 8.187 7.860 7.683 9.259 8.480 8.305 7.459 8.254 8.066 8.428 8.346 7.805 8.127 8.333 8.348 7.974 7.537 8.761
area 7aOH
3.471 2.911 0.024 0.537 0.542 n.d. 0.556 0.583 n.d. 2.127 2.288 n.d. 1.862 1.780 0.029 1.753 1.984 n.d. 0.368 0.396 n.d. 2.052 1.990 n.d. 0.496 0.455 n.d. 0.678 0.653 n.d. 1.697 1.674 0.029 6.870 6.821 0.075 1.183 1.091 0.011 0.546 0.559 n.d. 2.021 1.895 0.020 0.619 0.595 0.024 1.066 1.116 n.d. 2.606 2.667 0.010 3.369 3.371 n.d. 2.884 2.681 0.043
g 7aOH formed
0.212 0.197 0.002 0.035 0.031 0.000 0.032 0.036 0.000 0.128 0.141 0.000 0.110 0.112 0.002 0.105 0.122 0.000 0.026 0.023 0.000 0.120 0.123 0.000 0.028 0.028 0.000 0.040 0.036 0.000 0.100 0.099 0.002 0.431 0.407 0.004 0.071 0.069 0.001 0.036 0.034 0.000 0.123 0.121 0.001 0.033 0.035 0.000 0.071 0.068 0.000 0.155 0.160 0.001 0.207 0.202 0.000 0.181 0.178 0.002
7 alpha hydroxylase activity (nmol/h/mg protein) 3.751 0.614 0.621 2.485 2.023 2.096 0.455 2.246 0.521 0.700 1.815 7.668 1.275 0.646 2.229 0.633 1.284 2.892 3.783 3.268
Page A139 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
7.543 7.412 8.080 8.523 8.450 7.586 8.189 9.455 7.871 9.543 8.293 8.583 6.707 8.792 8.695 7.664 8.129 9.510 9.979 9.559 9.372 8.482 9.335 7.948 8.375 8.607 9.266 7.959 10.692 8.987 8.115 9.262 8.761 4.538 4.178 4.434 3.873 4.184 4.643 8.343 9.026 9.171 3.989 3.669 4.843 4.001 4.331 5.012 3.910 4.155 5.027 4.100 4.005 4.765 4.192 4.391 4.288 4.084 4.075 4.527 4.149 3.944 4.428
area 7aOH
4.629 4.514 0.158 4.801 4.542 0.494 0.921 0.949 0.014 1.148 0.974 0.026 3.465 4.504 0.064 6.853 7.362 n.d. 2.836 2.618 n.d. 0.848 0.932 n.d. 4.765 4.853 0.083 0.895 1.034 0.006 0.890 0.924 0.028 2.079 2.064 0.049 1.823 1.740 0.032 4.317 4.463 0.031 0.463 0.409 n.d. 1.789 1.905 0.014 0.450 0.453 n.d. 0.821 0.866 0.037 2.296 2.352 0.023 2.755 2.820 n.d. 1.052 1.025 0.036
g 7aOH formed
0.307 0.305 0.010 0.282 0.269 0.033 0.056 0.050 0.001 0.060 0.059 0.000 0.258 0.256 0.004 0.447 0.453 0.000 0.142 0.137 0.000 0.050 0.050 0.000 0.284 0.282 0.004 0.056 0.048 0.000 0.055 0.050 0.000 0.229 0.247 0.006 0.235 0.208 0.003 0.259 0.247 0.002 0.058 0.056 0.000 0.224 0.220 0.001 0.058 0.055 0.000 0.100 0.108 0.004 0.274 0.268 0.003 0.337 0.346 0.000 0.127 0.130 0.004
7 alpha hydroxylase activity (nmol/h/mg protein) 5.466 4.482 0.967 1.098 4.684 8.312 2.577 0.923 5.149 0.960 0.967 4.295 4.031 4.642 1.051 4.071 1.035 1.852 4.954 6.311 2.296
Page A140 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Mouse 39 49 59 69 10 20 40 60 30 50 70
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
4.145 4.132 3.371 4.812 3.305 4.276 3.994 3.969 4.551 3.328 3.792 10.237 9.887 11.010 8.101 9.894 12.152 11.089 10.748 10.280 11.854 9.866 10.231 11.422 10.852 10.771 11.044 10.087 10.560 10.687 10.035 10.174 10.971
n.d.: not detectable
area 7aOH
0.417 0.483 n.d. 1.356 0.979 n.d. 0.219 0.210 n.d. 0.710 0.735 n.d. 3.986 4.342 0.026 3.083 3.063 0.073 0.839 0.883 n.d. 0.951 0.893 0.040 2.471 2.346 n.d. 4.003 3.663 n.d. 1.536 1.606 n.d.
g 7aOH formed
0.050 0.058 0.000 0.141 0.148 0.000 0.027 0.026 0.000 0.107 0.097 0.000 0.202 0.197 0.002 0.156 0.126 0.003 0.039 0.043 0.000 0.048 0.044 0.002 0.114 0.109 0.000 0.198 0.173 0.000 0.077 0.079 0.000
7 alpha hydroxylase activity (nmol/h/mg protein) 1.004 2.669 0.498 1.880 3.654 2.542 0.757 0.816 2.057 3.435 1.436
Page A141 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Group
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
mouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
liver weight (g) 2.19 2.00 1.66 1.41 1.68 1.70 1.61 1.77 1.68 1.49
2.77 1.17 1.88 2.53 1.68 1.23 1.35 1.84 1.53 1.98
2.00 2.23 2.27 1.68 1.56 2.06 1.85 1.51 2.42 2.14
3.46 3.68 3.69 3.93 4.23 1.94 3.62 3.90 4.03 3.39
1.90 1.71 2.48 1.69 2.25 2.46 1.47 1.88 1.82 2.14
3.90 4.87 4.10 4.17 4.13 3.48 3.13 4.11 4.74 4.33
4.29 4.33 3.88 4.28 4.15 3.85 3.91 3.83 3.33 3.92
average mg microsomes/g liver
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
cholesterol 7 alpha hydroxylase activity
(nmol/h/mg protein) per liver (nmol/h)
1.681
45.14
2.296
56.32
2.282
46.47
2.485
42.97
2.892
59.59
1.815
37.83
4.684
92.49
4.295
93.24
4.954
102.07
3.654
66.76
3.104
64.29
1.206
23.55
2.023
68.74
7.668
110.02
2.959
68.22
1.137
35.27
3.783
77.94
8.312
125.39
2.898
47.98
4.031
90.96
6.311
118.43
2.542
61.74
4.166
80.47
2.442
30.16
2.662
65.31
1.373
37.55
2.346
65.30
2.096
43.19
1.275
24.40
3.268
82.56
2.577
58.48
4.642
85.97
2.296
68.15
2.057
54.00
2.459
58.49
0.965
19.37
0.785
33.32
0.456
20.60
1.014
45.89
0.455
21.91
0.646
33.51
5.466
130.06
0.923
40.97
1.051
50.26
1.004
49.65
0.757
31.48
1.256
45.77
1.495
31.40
3.751
87.40
2.246
47.11
2.229
67.81
4.482
92.91
2.661
73.43
1.093
32.96
5.149
92.83
4.071
93.86
2.669
59.59
3.435
90.15
3.179
73.80
1.221
21.60
0.658
31.48
0.368
22.00
0.614
30.88
0.521
26.64
0.633
32.05
0.967
41.25
0.960
36.84
1.035
52.17
0.498
28.93
0.816
43.33
0.707
34.56
0.226
8.94
0.576
30.32
0.472
25.05
0.621
29.54
0.700
36.74
1.284
65.37
1.098
51.84
0.967
46.38
1.852
86.98
1.880
76.80
1.436
69.03
1.089
51.81
0.516
21.79
Page A142 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
HMG CoA reductase activity measurements
internal standard mix
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2
mouse# 1 13 21 31 45 51 61 2 14 22 32 46 52 3 17 23 33 53 4 11 24 34 42 54 64 6 12 25 35
blanc blanc
3H dpm (internal standard)
14802 15138 14416 16500 16182 18200 16197 16086 15302 17401 19233 18151 18408 16484 14705 17314 no sample 19044 17259 17962 16815 18678 17627 16776 18636 13567 12055 12101 13870 12322 13846 15599 13354 14885 17744 17935 15926 15147 17598 18276 17701 13443 16084 13092 12500 12547 12475 15313 15836 11436 13617 15941 12877 13025 17786 15984 16769 15482 14676 12984
14C dpm
222 207 199 294 310 278 437 321 187 224 296 360 192 169 132 159
172 124 137 205 265 140 158 226 146 118 92 143 138 116 144 197 241 231 266 190 172 221 316 187 156 260 209 127 116 114 145 119 86 104 110 196 254 405 279 378 214 162 118
14C dpm -blanc 82 67 59 154 170 138 297 181 47 84 156 220 52 29 -8 19
14C mevalonate formed (dpm) 169 135 125 284 320 231 558 342 93 147 247 369 86 54 -17 33
HMG CoA activity pmol/min/mg protein
5.111 4.083 3.776 8.611 9.692 6.995 16.917 10.381 2.834 4.454 7.483 11.182 2.606 1.623 -0.502 1.012
32
51
-16
-28
-3
-5
65
118
125
204
0
0
18
33
86
140
6
13
-22
-56
-48
-121
3
7
-2
-5
-24
-53
4
8
57
130
101
207
91
156
126
214
50
96
32
64
81
140
176
293
47
81
16
36
120
227
69
160
-13
-32
-24
-58
-26
-63
5
10
-21
-40
-54
-144
-36
-80
-30
-57
56
132
114
266
265
453
139
247
238
403
74
136
1.550 -0.855 -0.154 3.566 6.174 0.000 0.990 4.257 0.408 -1.684 -3.659 0.200 -0.150 -1.599 0.237 3.938 6.260 4.731 6.481 2.896 1.949 4.246 8.885 2.450 1.098 6.883 4.862 -0.959 -1.765 -1.923 0.301 -1.223 -4.356 -2.439 -1.736 4.012 8.075 13.746 7.482 12.211 4.112
mean 4.60 6.19 8.34 13.65 3.64 9.33 2.11 0.26 1.55 0.00 4.87 0.49 2.33 0.00 0.02 0.00 5.10 5.61 2.42 6.57 1.77 5.87 0.00 0.00 0.00 0.00 6.04 10.61 8.16
Input internal standard (dpm): average
mix 1 30176 29765 31362
30434
mix 2 28810 28307 28031
28383
14C dpm HMG CoA mix: average
58465 57242 55833
57180 11436
per 5 l mix per l mix
HMG CoA mix: Specific activity 14C HMGCoA:
incubation time: liver microsomes:
2.6 4398.46
30.0 0.250
mmol/l HMG CoA dpm/nmol min mg
Page A143 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mouse# 43 55 65 5 15 26 36 44 66 7 16 27 37
blanc blanc
3H dpm (internal standard)
16023 14382 15354 16171 15593 15352 13631 12996 13907 11471 10075 12964 13289 16215 17106 14863 14921 14914 14706 15107 13433 14340 14736 15759 16357 15923 14228 11628
Input internal standard (dpm): average
27932 27694 28968
28198
14C dpm
252 192 261 211 451 136 140 139 98 109 97 93 139 173 402 338 155 168 195 321 191 287 142 154 204 179 194 144
14C dpm -blanc 83 23 92 42 282 -33 -29 -30 -71 -60 -72 -76 -30 4 233 169 -14 -1 26 152 22 118 -27 -15 35 10
14C mevalonate formed (dpm) 146 45 169 73 510 -61 -60 -65 -144 -147 -202 -165 -64 7 384 321 -26 -2 50 284 46 232 -52 -27 60 18
HMG CoA act. pmol/min.mg eiwit
4.237 1.308 4.901 2.124 14.792 -1.758 -1.740 -1.888 -4.176 -4.278 -5.845 -4.795 -1.846 0.202 11.141 9.300 -0.767 -0.055 1.446 8.230 1.340 6.731 -1.499 -0.779 1.750 0.514
mean 2.77 3.51 6.52 0.00 0.00 0.00 0.00 10.22 0.00 4.84 0.00 0.00 1.13
14C HMG CoA mix: average
58407 55714 65147
59756 11951.2
per 5 l mix per l mix
HMG CoA mix: Specific activity 14C HMGCoA:
incubation time: liver microsomes:
2.6 mmol/l HMG CoA
4596.62 dpm/nmol
30.0
min
0.250
mg
mouse# 67 8 9 19 29 39 49 59 50 70
blanc blanc
3H dpm (internal standard)
16112 16092 14295 17696 17286 16982 14651 19083 16755 16504 17225 17750 18651 15076 15831 16644 16807 14453 16679 15823 16121 16049
Input internal standard (dpm): average
27956 27723 28126
27935
14C dpm
284 180 129 164 379 317 145 113 142 115 247 218 209 152 159 154 195 321 191 287 95 72
14C dpm -blanc 201 97 46 81 296 234 62 30 59 32 164 135 126 69 76 71 112 238 108 204
14C mevalonate formed (dpm) 348 168 89 127 478 384 117 43 98 53 265 212 188 127 133 118 185 459 180 359
HMG CoA act. pmol/min.mg eiwit
9.646 4.648 2.467 3.526 13.251 10.658 3.254 1.198 2.706 1.479 7.358 5.874 5.216 3.522 3.697 3.283 5.142 12.738 4.996 9.969
mean 7.15 3.00 11.95 2.23 2.09 6.62 4.37 3.49 8.94 7.48
14C HMG CoA mix: average
63731 60065 63603
62466 12493.27
per 5 l mix per l mix
HMG CoA mix: Specific activity 14C HMGCoA:
incubation time: liver microsomes:
2.6 mmol/l HMG CoA
4805.1
dpm/nmol
30.0
min
0.250
mg
Page A144 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
mouse# 18 28 38 48 58 68 69 10 20 40 30 62 41 63 56 47
blanc
3H dpm (internal standard)
18621 18163 16885 17274 15495 18485 15821 17041 19175 17310 16607 14530 16586 14581 15189 15445 16021 16747 15999 14205 20405 19844 16228 14978 15343 13316 15166 16339 17431 14614 15456 15823 16766
Input internal standard (dpm): average
32959 31221 31297
31826
14C dpm
513 361 265 260 682 691 341 312 412 318 241 348 352 360 275 241 224 235 404 355 591 403 328 218 233 317 214 302 407 514 650 287 164
14C dpm -blanc 349 197 101 96 518 527 177 148 248 154 77 184 188 196 111 77 60 71 240 191 427 239 164 54 69 153 50 138 243 350 486 123
14C mevalonate formed (dpm) 596 345 190 177 1064 907 356 276 412 283 148 403 361 428 233 159 119 135 477 428 666 383 322 115 143 366 105 269 444 762 1001 247
HMG CoA act. pmol/min.mg eiwit
17.513 10.135 5.589 5.193 31.238 26.640 10.454 8.115 12.085 8.313 4.333 11.833 10.592 12.561 6.829 4.658 3.499 3.962 14.017 12.564 19.554 11.254 9.443 3.369 4.202 10.736 3.081 7.892 13.026 22.379 29.382 7.264
mean 13.82 5.39 28.94 9.28 10.20 8.08 11.58 5.74 3.73 13.29 15.40 6.41 7.47 5.49 17.70 18.32
14C HMG CoA mix: average
61119 60406 55583
59036 11807.2
per 5 l mix per l mix
HMG CoA mix: Specific activity 14C HMGCoA:
incubation time: liver microsomes:
2.6 mmol/l HMG CoA
4541.23 dpm/nmol
30.0
min
0.250
mg
Page A145 of A172
Appendix to TNO-report of 3M-02
Final version
Group mouse#
1
1
1
2
1
3
1
4
1
5
1
6
1
7
1
8
1
9
1
10
AVERAGE
SD
2
11
2
12
2
13
2
14
2
15
2
16
2
17
2
18
2
19
2
20
AVERAGE
SD
3
21
3
22
3
23
3
24
3
25
3
26
3
27
3
28
3
29
3
30
AVERAGE
SD
4
31
4
32
4
33
4
34
4
35
4
36
4
37
4
38
4
39
4
40
AVERAGE
SD
5
41
5
42
5
43
5
44
5
45
5
46
5
47
5
48
5
49
5
50
AVERAGE
SD
6
51
6
52
6
53
6
54
6
55
6
56
6
57
6
58
6
59
6
60
AVERAGE
SD
7
61
7
62
7
63
7
64
7
65
7
66
7
67
7
68
7
69
7
70
AVERAGE
SD
liver weight (g)
2.19 2.00 1.66 1.41 1.68 1.70 1.61 1.77 1.68 1.49
average mg microsomes/g liver
12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26 12.26
2.77
12.26
1.17
12.26
1.88
12.26
2.53
12.26
1.68
12.26
1.23
12.26
1.35
12.26
1.84
12.26
1.53
12.26
1.98
12.26
2.00
12.26
2.23
12.26
2.27
12.26
1.68
12.26
1.56
12.26
2.06
12.26
1.85
12.26
1.51
12.26
2.42
12.26
2.14
12.26
3.46
12.26
3.68
12.26
3.69
12.26
3.93
12.26
4.23
12.26
1.94
12.26
3.62
12.26
3.90
12.26
4.03
12.26
3.39
12.26
1.90
12.26
1.71
12.26
2.48
12.26
1.69
12.26
2.25
12.26
2.46
12.26
1.47
12.26
1.88
12.26
1.82
12.26
2.14
12.26
3.90
12.26
4.87
12.26
4.10
12.26
4.17
12.26
4.13
12.26
3.48
12.26
3.13
12.26
4.11
12.26
4.74
12.26
4.33
12.26
4.29
12.26
4.33
12.26
3.88
12.26
4.28
12.26
4.15
12.26
3.85
12.26
3.91
12.26
3.83
12.26
3.33
12.26
3.92
12.26
HMG CoA reductase activity
(pmol/min/mg protein) per liver (pmol/min)
4.60
123.43
0.26
6.26
0.00
0.00
2.42
41.88
0.00
0.00
0.00
0.00
4.84
95.49
3.00
65.03
11.95
246.23
5.74
104.92
3.28
68.32
3.77
78.41
6.57
222.96
6.04
86.69
6.19
142.75
1.55
48.08
0.00
0.00
0.00
0.00
0.02
0.41
13.82
311.85
2.23
41.76
3.73
90.56
4.02
94.51
4.33
104.24
8.34
204.59
0.00
0.00
0.00
0.00
1.77
36.54
10.61
203.00
0.00
0.00
0.00
0.00
5.39
99.81
2.09
62.10
15.40
404.15
4.36
101.02
5.44
133.13
13.65
578.98
4.87
219.73
5.10
230.67
5.87
282.96
8.16
423.27
0.00
0.00
1.13
50.24
28.94
1383.68
6.62
326.87
13.29
552.38
8.76
404.88
8.36
392.39
7.47
173.99
0.00
0.00
2.77
84.30
10.22
211.77
3.64
100.51
0.49
14.93
18.32
330.22
9.28
214.00
4.37
97.48
8.94
234.56
6.55
146.18
5.51
104.48
9.33
446.23
2.33
139.28
5.61
281.81
0.00
0.00
3.51
177.86
17.70
755.28
6.15
236.05
10.20
513.93
3.49
202.82
6.69
355.05
6.50
310.83
5.01
216.26
2.11
111.22
6.41
340.07
5.49
260.98
0.00
0.00
6.52
331.58
0.00
0.00
7.15
342.61
8.08
379.53
11.58
472.60
7.48
359.61
5.48
259.82
3.71
165.03
04-12-2006
Page A146 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
micromes
mg/g liver 7-alpha act. 7-alpha act. per liver HMG Co A act. HMG Co A act. per liver
micromes
mg/g liver 7-alpha act. 7-alpha act. per liver HMG Co A act. HMG Co A act. per liver
micromes
mg/g liver 7-alpha act. 7-alpha act. per liver HMG Co A act. HMG Co A act. per liver
micromes; mg per group 1 vs 2 nn p
10 10 0.971 10 10 0.353 10 10 0.190 10 10 0.631 10 10 0.739
group 2 vs 4 nn p 10 10 0.218 10 10 <0.001 10 10 0.009 10 10 0.190 10 10 0.015 group 3 vs 7 nn p 10 10 0.796 10 10 0.001 10 10 0.631 10 10 0.436 10 10 0.063
g liver, 7-alpha-hydroxylase activity and HMG CoA group 1 vs 3 group 1 vs 4 group 1 vs 5 nn p nn p nn p
10 10 0.529 10 10 0.143 10 10 0.739 10 10 0.393 10 10 0.002 10 10 0.912 10 10 0.684 10 10 0.035 10 10 0.353 10 10 1.000 10 10 0.029 10 10 0.165 10 10 1.000 10 10 0.007 10 10 0.105
group 2 vs 5 group 2 vs 6 group 2 vs 7 nn p nn p nn p 10 10 0.579 10 10 0.579 10 10 0.315 10 10 0.529 10 10 <0.001 10 10 <0.001 10 10 0.684 10 10 <0.001 10 10 0.043 10 10 0.247 10 10 0.247 10 10 0.315 10 10 0.247 10 10 0.015 10 10 0.035 group 4 vs 5 group 4 vs 6 group 4 vs 7 nn p nn p nn p 10 10 0.063 10 10 0.029 10 10 0.029 10 10 0.002 10 10 0.529 10 10 0.529 10 10 0.015 10 10 0.353 10 10 0.353 10 10 0.631 10 10 0.684 10 10 0.579 10 10 0.043 10 10 0.631 10 10 0.684
reductase activity,
group 1 vs 6
nn
p
10 10 0.971
10 10 <0.001
10 10 <0.001
10 10 0.105
10 10 0.003
group 3 vs 4
nn
p
10 10 0.035
10 10 0.002
10 10 0.052
10 10 0.218
10 10 0.011
group 5 vs 6
nn
p
10 10 0.481
10 10 <0.001
10 10 <0.001
10 10 0.912
10 10 0.052
p values Mann-Whitney U-test group 1 vs 7 group 2 vs 3 nn p nn p
10 10 0.315 10 10 0.436 10 10 <0.001 10 10 0.105 10 10 0.247 10 10 0.089 10 10 0.165 10 10 0.796 10 10 0.019 10 10 0.739
group 3 vs 5 group 3 vs 6 nn p nn p 10 10 0.912 10 10 0.738 10 10 0.190 10 10 <0.001 10 10 0.075 10 10 0.005 10 10 0.280 10 10 0.218 10 10 0.190 10 10 0.023 group 5 vs 7 group 6 vs 7 nn p nn p 10 10 0.971 10 10 0.143 10 10 <0.001 10 10 0.143 10 10 0.029 10 10 0.123 10 10 0.684 10 10 0.912 10 10 0.063 10 10 0.853
p<0.05
Page A147 of A172
Appendix to TNO-report of 3M-02
Final version
H2. Cholesterol-27-hydroxylase activity in mitochondria
Protein concentration isolated mitochondria
Mitochondria
mouse
1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29 39 49 59 69 10 20 40 60 30 50 70
measured prot conc (mg/ml)
well 1
well 2
0.772
0.8
0.699
0.699
0.848
0.908
1.508
1.499
0.925
0.973
0.784
0.813
0.865
0.881
1.143
1.162
0.918
0.931
0.945
1.022
1.192
1.104
1.043
1.062
1.26
1.236
1.201
1.204
0.81
0.81
0.57
0.54
0.915
0.949
1.036
1.092
0.699
0.655
1.033
0.942
0.898
0.839
0.576
0.587
0.726
0.672
0.969
0.959
1.532
1.43
0.784
0.852
0.98
0.997
1.201
1.28
0.699
0.658
0.383
0.359
0.931
0.861
1.37
1.235
0.842
0.845
0.945
0.976
0.949
0.901
0.649
0.637
0.648
0.635
0.775
0.799
0.678
0.705
0.757
0.757
0.84
0.843
1.031
0.998
0.754
0.714
0.467
0.443
0.876
0.886
1.171
1.1
0.554
0.554
0.847
0.809
0.578
0.596
0.616
0.596
0.697
0.7
0.728
0.745
1.808
1.903
1.086
0.85
1.261
1.147
0.734
0.766
0.616
0.611
0.691
0.659
0.847
0.847
1.255
1.251
0.76
0.751
0.85
0.796
0.751
0.728
0.837
0.821
0.84
0.809
1.344
1.34
0.769
0.827
0.642
0.598
0.987
0.965
1.039
0.926
dilution
50 50 50 50 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 50 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 50 100 50 100 100 50 50 100 100 50 50 100
Protein conc (mg/ml) 39.30 34.95 43.90 75.18 47.45 79.85 87.30 57.63 46.23 49.18 114.80 52.63 124.80 120.25 40.50 27.75 46.60 106.40 33.85 98.75 86.85 29.08 34.95 48.20 148.10 40.90 98.85 124.05 33.93 18.55 44.80 130.25 42.18 96.05 92.50 32.15 32.08 39.35 34.58 37.85 84.15 101.45 36.70 22.75 44.05 113.55 27.70 82.80 58.70 30.30 34.93 36.83 185.55 48.40 120.40 75.00 30.68 33.75 42.35 125.30 37.78 82.30 73.95 41.45 41.23 134.20 79.80 31.00 48.80 98.25
04-12-2006
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Appendix to TNO-report of 3M-02
Final version
Amount isolated mitochondria
group#
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
mouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
Protein conc mitochondria (mg/ml)
39.30
57.63 40.50 29.08 32.15 33.93 36.70 30.30 30.68 41.45
estimated l microsomes
600
600 600 600 600 600 600 600 600 600
34.95
600
18.55
600
34.95
600
46.23
600
32.08
600
22.75
600
27.75
600
34.93
600
33.75
600
41.23
600
43.90
600
49.18
600
46.60
600
48.20
600
44.80
600
39.35
600
44.05
600
36.83
600
42.35
600
31.00
600
75.18
700
114.80
700
106.40
700
148.10
700
130.25
700
34.58
600
113.55
700
185.55
700
125.30
700
134.20
700
33.85
600
40.90
600
42.18
600
37.85
600
47.45
600
52.63
600
27.70
600
48.40
600
37.78
600
48.80
600
79.85
700
124.80
700
98.75
700
98.85
700
96.05
700
84.15
700
82.80
700
120.40
700
82.30
700
79.80
700
87.30
700
120.25
700
86.85
700
124.05
700
92.50
700
101.45
700
58.70
700
75.00
700
73.95
700
98.25
700
isolated mitochondria (mg) 23.58 34.58 24.30 17.45 19.29 20.36 22.02 18.18 18.41 24.87
20.97 11.13 20.97 27.74 19.25 13.65 16.65 20.96 20.25 24.74
26.34 29.51 27.96 28.92 26.88 23.61 26.43 22.10 25.41 18.60
52.62 80.36 74.48 103.67 91.18 20.75 79.49 129.89 87.71 93.94
20.31 24.54 25.31 22.71 28.47 31.58 16.62 29.04 22.67 29.28
55.90 87.36 69.13 69.20 67.24 58.91 57.96 84.28 57.61 55.86
61.11 84.18 60.80 86.84 64.75 71.02 41.09 52.50 51.77 68.78 64.28 14.24
used liver for isolation (g)
1.92 1.76 1.45 1.19 1.49 1.48 1.42 1.6 1.52 1.31
2.52 1.04 1.8 2.23 1.48 1.05 1.16 1.67 1.34 1.79
1.76 1.98 1.97 1.5 1.39 1.78 1.67 1.35 2.18 1.14
3.1 3.33 3.33 3.53 3.89 1.72 3.21 3.43 3.64 3.19
1.71 1.51 2.32 1.47 2.14 2.17 1.32 1.65 1.62 1.86
3.49 4.36 4.1 4.17 4.13 3.13 2.78 3.57 4.17 3.85
3.85 3.86 3.27 3.92 3.7 3.48 3.27 3.36 2.92 3.48
mg mitochondria per g liver 12.28 19.64 16.76 14.66 12.95 13.75 15.51 11.36 12.11 18.98 14.80 2.89 8.32 10.70 11.65 12.44 13.00 13.00 14.35 12.55 15.11 13.82 12.49 1.94 14.97 14.90 14.19 19.28 19.34 13.26 15.83 16.37 11.66 16.32 15.61 2.42 16.98 24.13 22.37 29.37 23.44 12.06 24.76 37.87 24.10 29.45 24.45 7.03 11.88 16.25 10.91 15.45 13.30 14.55 12.59 17.60 13.99 15.74 14.23 2.09 16.02 20.04 16.86 16.59 16.28 18.82 20.85 23.61 13.82 14.51 17.74 3.05 15.87 21.81 18.59 22.15 17.50 20.41 12.57 15.63 17.73 19.76 18.20 2.99
AVERAGE SD
group 1-3,5 14.28 2.55
AVERAGE SD
group 4 24.45 7.03
AVERAGE SD
group 6,7 17.97 2.95
04-12-2006
Page A149 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Cholesterol-27-hydroxylase activity measurements
used per assay 420 g protein 20 min incubation
internal standard 20 OH: 0.5 g per determination
Mouse 1 13 21 31 45 51 61 2 14 22 32 46 52 62 3 17 23 33
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
10.798 10.39 7.98 11.806 12.439 8.742 11.5 11.782 8.867 9.271 10.042 8.477 12.431 12.184 8.816 10.388 10.595 8.259 9.893 10.305 8.75 9.624 10.36 8.95 11.022 11.6 9.308 10.919 10.512 9.52 8.987 9.298 8.538 10.614 10.223 9.715 9.353 10.539 8.851 10.024 9.352 8.92 12.459 11.384 9.006 10.383 14.276 9.026 9.646 10.293 7.233 8.216 8.156 7.185
area 27OH
13.936 13.174 0.136 20.962 19.566 0.125 19.885 16.14 0.126 8.456 9.881 0.038 18.514 17.151
0.07 10.217 11.997 0.113 11.615 11.839 0.068 11.398 13.32
n.a. 14.253 14.61 0.023 15.555 16.346 0.074 8.043 8.372 0.023 15.727 15.178 0.048 9.827 9.649 0.071 6.711 6.677
n.a. 19.765 15.393
n.a. 12.005 18.144
n.a. 14.544 14.524
n.a. 7.206 6.744 0.249
g 27 OH formed
0.645 0.634 0.009 0.888 0.786 0.007 0.865 0.685 0.007 0.456 0.492 0.002 0.745 0.704 0.004 0.492 0.566 0.007 0.587 0.574 0.004 0.592 0.643 0.000 0.647 0.630 0.001 0.712 0.777 0.004 0.447 0.450 0.001 0.741 0.742 0.002 0.525 0.458 0.004 0.335 0.357 0.000 0.793 0.676 0.000 0.578 0.635 0.000 0.754 0.706 0.000 0.439 0.413 0.017
27- hydroxylase activity (nmol/h/mg protein) 11.659 15.332 14.181 8.715 13.306 9.645 10.656 11.407 11.766 13.689 8.267 13.654 9.007 6.389 13.571 11.209 13.480 7.549
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Final version
04-12-2006
Mouse 41 53 63 4 11 24 34 42 54 64 6 12 25 35 43 55 65 5 15 26
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
10.176 9.541 7.88 7.856 7.979 7.279 10.998 8.797 7.705 9.576 10.192 7.719 11.137 9.797 8.556 10.407 9.642 6.778 6.577 9.528 7.252 9.538 9.074 7.471 8.887 8.267 7.436 8.585 8.945 6.454 11.823 8.846 8.775 9.224 9.098 9.234 10.72 9.542 8.864 9.856 10.84 8.053 10.178 10.114 7.765 8.696 8.713 8.251 9.579 9.473 8.811 9.569 9.56 8.442 10.061 10.559 9.105 9.863 9.469 8.926
area 27OH
4.943 4.889 n.a. 4.981 4.873 n.a. 7.897 6.181 0.086 11.39 12.068 n.a. 8.145 7.601 0.038 11.002 11.512 n.a. 3.674 5.095 n.a. 11.731 10.387 0.027 6.229 5.477 n.a. 6.457 6.783 n.a. 13.325 9.816 0.057 1.784 1.721 n.a. 7.132 7.327 0.031 5.695
4.8 n.a. 12.222 10.741 n.a. 5.552 5.349 0.032 6.842 7.217 0.056 7.618 7.922 0.004 8.488 7.934 n.a. 7.929 8.269 n.a.
g 27 OH formed
0.243 0.256 0.000 0.317 0.305 0.000 0.359 0.351 0.006 0.595 0.592 0.000 0.366 0.388 0.002 0.529 0.597 0.000 0.279 0.267 0.000 0.615 0.572 0.002 0.350 0.331 0.000 0.376 0.379 0.000 0.564 0.555 0.003 0.097 0.095 0.000 0.333 0.384 0.000 0.289 0.221 0.000 0.600 0.531 0.000 0.319 0.307 0.002 0.357 0.381 0.003 0.398 0.414 0.000 0.422 0.376 0.000 0.402 0.437 0.000
27- hydroxylase activity (nmol/h/mg protein) 4.610 5.749 6.458 10.962 6.920 10.396 5.049 10.933 6.297 6.976 10.270 1.767 6.619 4.714 10.450 5.748 6.758 7.499 7.366 7.746
Page A151 of A172
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Final version
04-12-2006
Mouse 36 44 56 66 7 16 27 37 47 57 67 8 18 28 38 48 58 68 9 19 29
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
9.147 9.469 7.56 9.972 11.897 8.394 9.607 9.685 8.852 9.829 10.203 9.701 10.39 9.963 9.004 9.887 9.808 8.749 10.948 11.307 9.47 10.004 9.859 8.538 9.054 10.166 9.566 10.482 9.237 9.439 9.185 9.321 8.555 10.123 10.395 8.227 9.534 9.641 7.802 10.422 10.894 7.384 9.189 8.998 8.56 10.497 10.009 8.178 8.418 9.913 8.293 9.401 10.176 8.704 8.909 9.904 7.036 6.336 8.809 6.461 6.725 9.786 4.961
area 27OH
4.853 4.856 0.022 8.19 8.239 n.a. 5.319 4.876 0.035 7.898 7.893 0.042 6.813 7.322 n.a. 6.158 5.884 n.a. 8.509 8.456 0.018 6.283 6.029 n.a. 6.579 8.773 n.a. 5.812 5.663 n.a. 8.637 10.313 0.041 14.048 11.26 n.a. 9.63 8.353 n.a. 10.021 12.138 n.a. 3.667 3.925 n.a. 11.218 9.378 0.01 4.05 4.887 n.a. 6.674 6.558 n.a. 7.488 10.247 n.a. 5.573 7.57 n.a. 5.613 7.53 0.002
g 27 OH formed
0.265 0.256 0.001 0.411 0.346 0.000 0.277 0.252 0.002 0.402 0.387 0.002 0.328 0.367 0.000 0.311 0.300 0.000 0.389 0.374 0.001 0.314 0.306 0.000 0.363 0.431 0.000 0.277 0.307 0.000 0.470 0.553 0.002 0.694 0.542 0.000 0.505 0.433 0.000 0.481 0.557 0.000 0.200 0.218 0.000 0.534 0.468 0.001 0.241 0.246 0.000 0.355 0.322 0.000 0.420 0.517 0.000 0.440 0.430 0.000 0.417 0.385 0.000
27- hydroxylase activity (nmol/h/mg protein) 4.792 6.991 4.846 7.244 6.422 5.647 7.026 5.725 7.341 5.392 9.408 11.412 8.666 9.586 3.858 9.251 4.499 6.255 8.660 8.031 7.405
Page A152 of A172
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Final version
04-12-2006
Mouse 39 49 59 69 10 20 40 60 30 50 70
energy generating mix added
+ NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix + NADPH mix + NADPH mix - NADPH mix
area 20 OH
8.832 9.279 8.084 9.633 11.234 7.927 9.799 9.597 9.087 9.836 9.96 1.235 9.549 10.004 7.526 9.543 9.019 8.008 7.696 8.096 7.695 8.639 8.551 8.104 8.271 8.406 7.768 9.353 9.445 7.913 9.568 8.325 7.941
area 27OH
4.203 4.385 n.a. 6.413 7.141 n.a. 4.515 4.761 n.a. 6.674 6.877 n.a. 8.229 9.15 n.a. 7.346 7.444 0.031 2.057 2.046 0.01 3.465 3.757 0.047 5.462 5.704 n.a. 8.537 7.619 n.a. 4.626 4.716 n.a.
g 27 OH formed
0.238 0.236 0.000 0.333 0.318 0.000 0.230 0.248 0.000 0.339 0.345 0.000 0.431 0.457 0.000 0.385 0.413 0.002 0.134 0.126 0.001 0.201 0.220 0.003 0.330 0.339 0.000 0.456 0.403 0.000 0.242 0.283 0.000
27- hydroxylase activity (nmol/h/mg protein) 4.380 6.010 4.419 6.322 8.204 7.331 2.390 3.828 6.184 7.941 4.849
Page A153 of A172
Appendix to TNO-report of 3M-02
Final version
Group
1 1 1 1 1 1 1 1 1 1 AVERAGE SD 2 2 2 2 2 2 2 2 2 2 AVERAGE SD 3 3 3 3 3 3 3 3 3 3 AVERAGE SD 4 4 4 4 4 4 4 4 4 4 AVERAGE SD 5 5 5 5 5 5 5 5 5 5 AVERAGE SD 6 6 6 6 6 6 6 6 6 6 AVERAGE SD 7 7 7 7 7 7 7 7 7 7 AVERAGE SD
Mouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
mouse#
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
61 62 63 64 65 66 67 68 69 70
liver weight (g) 2.19 2.00 1.66 1.41 1.68 1.70 1.61 1.77 1.68 1.49
2.77 1.17 1.88 2.53 1.68 1.23 1.35 1.84 1.53 1.98
2.00 2.23 2.27 1.68 1.56 2.06 1.85 1.51 2.42 2.14
3.46 3.68 3.69 3.93 4.23 1.94 3.62 3.90 4.03 3.39
1.90 1.71 2.48 1.69 2.25 2.46 1.47 1.88 1.82 2.14
3.90 4.87 4.10 4.17 4.13 3.48 3.13 4.11 4.74 4.33
4.29 4.33 3.88 4.28 4.15 3.85 3.91 3.83 3.33 3.92
average mg mitochondria/g liver
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
24.45 24.45 24.45 24.45 24.45 24.45 24.45 24.45 24.45 24.45
14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28 14.28
17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97
17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97 17.97
cholesterol 27 hydroxylase activity
(nmol/h/mg protein) per liver (nmol/h)
11.66
364.7
11.41
325.9
13.57
321.8
10.96
220.8
7.50
180.0
10.27
249.4
6.42
147.7
11.41
288.5
8.66
207.8
8.20
174.6
10.01
248.1
2.22
73.9
6.92
273.8
1.77
29.5
15.33
411.7
11.77
425.2
7.37
176.8
5.65
99.2
11.21
216.1
8.67
227.8
8.03
175.5
7.33
207.3
8.40
224.3
3.70
123.2
14.18
405.1
13.69
436.0
13.48
437.1
10.40
249.5
6.62
147.5
7.75
227.9
7.03
185.6
9.59
206.8
7.40
255.9
6.18
189.0
9.63
274.0
3.14
109.9
8.72
737.3
8.27
743.8
7.55
681.1
5.05
485.2
4.71
487.5
4.79
227.3
5.72
506.7
3.86
367.9
4.38
431.6
2.39
198.1
5.54
486.7
2.03
192.9
4.61
125.1
10.93
267.0
10.45
370.2
6.99
168.8
13.31
427.6
13.65
479.8
7.34
154.1
9.25
248.4
6.01
156.2
7.94
242.7
9.05
264.0
3.02
123.5
9.65
676.0
9.01
788.2
5.75
423.5
6.30
471.8
5.75
426.6
4.85
303.0
5.39
303.3
4.50
332.3
4.42
376.4
3.83
297.8
5.94
439.9
1.93
167.2
10.66
821.5
6.39
497.1
6.46
450.3
6.98
536.5
6.76
504.0
7.24
501.1
9.41
661.0
6.25
430.5
6.32
378.3
4.85
341.6
7.13
512.2
1.68
140.0
04-12-2006
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Final version
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mitochondria
mg/g liver 27 hydroxylase act. 27 hydroxylase act. per liver
mitochondria
mg/g liver 27 hydroxylase act. 27 hydroxylase act. per liver
mitochondria
mg/g liver 27 hydroxylase act. 27 hydroxylase act. per liver
mitochondria; mg per g liver and 27-hydroxylase activity, p values Mann-Whitney U-test
group 1 vs 2 group 1 vs 3 group 1 vs 4 group 1 vs 5
group 1 vs 6
group 1 vs 7
nn p nn p nn p nn p nn
p
nn p
10 10 0.165 10 10 0.436 10 10 0.002 10 10 0.796 10 10 0.043 10 10 0.019
10 10 0.247 10 10 0.631 10 10 0.003 10 10 0.353 10 10 0.001 10 10 0.004
10 10 0.579 10 10 0.684 10 10 0.001 10 10 0.971 10 10 0.002 10 10 <0.001
group 2 vs 4 group 2 vs 5 group 2 vs 6 group 2 vs 7
group 3 vs 4
group 3 vs 5
nn p nn p nn p nn p nn
p
nn p
10 10 <0.001 10 10 0.075 10 10 <0.001 10 10 <0.001 10 10 0.002 10 10 0.218
10 10 0.075 10 10 0.796 10 10 0.063 10 10 0.165 10 10 0.005 10 10 0.684
10 10 0.003 10 10 0.631 10 10 0.002 10 10 <0.001 10 10 0.015 10 10 0.739
group 3 vs 7 group 4 vs 5 group 4 vs 6 group 4 vs 7
group 5 vs 6
group 5 vs 7
nn p nn p nn p nn p nn
p
nn p
10 10 0.052 10 10 0.001 10 10 0.009 10 10 0.011 10 10 0.011 10 10 0.005
10 10 0.043 10 10 0.015 10 10 0.529 10 10 0.075 10 10 0.019 10 10 0.123
10 10 0.001 10 10 0.009 10 10 0.393 10 10 0.796 10 10 0.009 10 10 <0.001
group 2 vs 3 nn p 10 10 0.005 10 10 0.579 10 10 0.353 group 3 vs 6 nn p 10 10 0.123 10 10 0.003 10 10 0.019 group 6 vs 7 nn p 10 10 0.739 10 10 0.035 10 10 0.089
p<0.05
Page A155 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
I. Measurements in vitro experiment (rat hepatocytes)
Experiment 1 Protein concentration determination
sample 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Measured protein conc (mg/ml)
97.5 90.6 119.5 125.5 172.9 140.5 155.8 180.3 167.7 177.1 131.6 121.2 139.7 114.5 257.5 214.7 176 175 155.8 141.6 155.8 122.1 167.7 154.8 129 127.2 136.1 141.5 170.8 177.1 172.9 185.7 168.7 155.6 177.1 165.7 151.9 150.9 188.9 183.5 190.1 229.9 168.7 162.7 167.7 157.7 229.9 218.4 150.9 146.2 127.2 133.4 172.9 151.9 170.8 185.7 170.8 178.1 163.7 170.8
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 376.2 490.0 626.8 672.2 689.6 505.6 508.4 944.4 702.0 594.8 555.8 645.0 512.4 555.2 695.8 717.2 648.6 685.6 605.6 744.8 840.0 662.8 650.8 896.6 594.2 521.2 649.6 713.0 697.8 669.0
g protein per well 125.4 163.3 208.9 224.1 229.9 168.5 169.5 314.8 234.0 198.3 185.3 215.0 170.8 185.1 231.9 239.1 216.2 228.5 201.9 248.3 280.0 220.9 216.9 298.9 198.1 173.7 216.5 237.7 232.6 223.0
sample 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
Measured protein conc (mg/ml)
134.3 127.2 149.9 131.6 202.7 195.7 194.1 216.8 188.7 203.7 173.8 161.3 194.1 216.8 188.7 203.8 173.9 161.3 168.6 173.9 140.8 143.8 139.7 144.8 155.1 147.9 162.4 164.4 164.4 160.3 179.2 169.7 198.4 167.6 209.1 195.1 207 216.8 239.9 255.6 241.1 249.9 182.33 132.7 201.6 195.1 212.4 221.2 210.4 193 211.4 243.3 208.1 205.9 239.9 223.4 213.5 211.4
198.4 181.3 129.7 133.7 156.1 181.3
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 523.0 563.0 796.8 821.8 784.8 670.2 821.8 785.0 670.4 685.0 569.2 569.0 606.0 653.6 649.4 697.8 732.0 808.4 847.6 991.0 982.0 630.1 793.4 867.2 806.8 909.4 828.0 926.6 849.8 759.4 526.8 674.8
g protein per well 174.3 187.7 265.6 273.9 261.6 223.4 273.9 261.7 223.5 228.3 189.7 189.7 202.0 217.9 216.5 232.6 244.0 269.5 282.5 330.3 327.3 210.0 264.5 289.1 268.9 303.1 276.0 308.9 283.3 253.1 175.6 224.9
Page A156 of A172
Appendix to TNO-report of 3M-02
Final version
sample 63 64 63 64 63 64 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107
Measured protein conc (mg/ml)
171.8 193 146.9 140.8 166.5 177 147.9 132.7 130.7 127.7 135.7 145.9 131.7 139.7 77.6 72.1 153.1 160.2 132.7 126.7 96.4 90.2 118.4 125.3 162.2 140.6 152.9 177.5 165.9 175.4 129.7 119.4 138.7 113.3 261.1 217.1 176.4 175.4 153.9 138.7 154.8 120.1 167.9 154.9 126.1 125.3 133.2 140.6 169 176.1 174.3 186.3 167.9 155.9 176.4 164.9 150 149 188.5 184.1 189.6 221.4 166.9 161.9 166.9 156.9 230.1 218.4 149 145.2 125.3 132.3 172.2 151.9 170.1 185.2 170.1 176.4 163.9 170.1 132.3 126.1 148.1 130.6 203.6 196.5 212.1 195.4 146.2 213.4
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 729.6 575.4 687.0 561.2 516.8 563.2 542.8 299.4 626.6 518.8 373.2 487.4 605.6 660.8 682.6 498.2 504.0 956.4 703.6 585.2 549.8 645.6 502.8 547.6 690.2 721.2 647.6 682.6 598.0 745.2 822.0 657.6 647.6 897.0 588.4 515.2 648.2 710.6 693.0 668.0 516.8 557.4 800.2 815.0 719.2
g protein per well 243.2 191.8 229.0 187.1 172.3 187.7 180.9 99.8 208.9 172.9 124.4 162.5 201.9 220.3 227.5 166.1 168.0 318.8 234.5 195.1 183.3 215.2 167.6 182.5 230.1 240.4 215.9 227.5 199.3 248.4 274.0 219.2 215.9 299.0 196.1 171.7 216.1 236.9 231.0 222.7 172.3 185.8 266.7 271.7 239.7
04-12-2006
Page A157 of A172
Page A158 of A172
sample compound concentration
1
control
2
control
3
control
4
control
5
control
6
control
7
control
8
control
9
control
10
control
11
control
12
control
13
PFBS 1 M
14
PFBS 1 M
15
PFBS 10 M
16
PFBS 10 M
17
PFBS 100 M
18
PFBS 100 M
19
PFHS 1 M
20
PFHS 1 M
21
PFHS 10 M
22
PFHS 10 M
23
PFHS 100 M
24
PFHS 100 M
25
PFOS 1 M
26
PFOS 1 M
27
PFOS 10 M
28
PFOS 10 M
29
PFOS 100 M
30
PFOS 100 M
31
Rosuvastatin 0.003 M
32
Rosuvastatin 0.003 M
33
Rosuvastatin 0.03 M
34
Rosuvastatin 0.03 M
35
Rosuvastatin 0.3 M
36
Rosuvastatin 0.3 M
37
PFBS 1 M
38
PFBS 1 M
39
PFBS 10 M
40
PFBS 10 M
41
PFBS 100 M
42
PFBS 100 M
43
PFHS 1 M
44
PFHS 1 M
45
PFHS 10 M
46
PFHS 10 M
47
PFHS 100 M
48
PFHS 100 M
49
PFOS 1 M
50
PFOS 1 M
51
PFOS 10 M
52
PFOS 10 M
53
PFOS 100 M
54
PFOS 100 M
14C Label
14C acetic acid 14C acetic acid 14C acetic acid
14C butyric acid 14C butyric acid 14C butyric acid
14C lauric acid 14C lauric acid 14C lauric acid
14C palmitic acid 14C palmitic acid 14C palmitic acid
14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid
14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid
3H-cholesterol 14C-cholesterol 14C-cholesterol corrected g protein
14C-cholesterol
Average14C-cholesterol % of control Average
SD
(dpm)
(dpm)
for recovery (dpm)
per mg protein (dpm/mg) per mg protein (dpm/mg)
% of control
12705
7957
16067
125.4
128130
129029
99
100
0.7
12827
10612
21225
163.3
129974
101
11820
12414
26944
208.9
128982
100
14124
8971
16295
224.1
72713
108158
67
100
34.2
12699
11979
24200
229.9
105265
97
15542
14954
24684
168.5
146495
135
12531
17327
35474
169.5
209286
156534
134
100
31.0
11864
16561
35812
314.8
113761
73
11644
15565
34294
234
146556
94
14154
7968
14442
198.3
72832
70783
103
100
19.5
12949
7804
15462
185.3
83441
118
14391
6763
12056
215
56077
79
15327
11500
19249
170.8
112700
110093
87
85
2.9
14468
11220
19896
185.1
107486
83
14439
13928
24747
231.9
106714
105182
83
82
1.7
18148
17531
24783
239.1
103650
80
12484
10821
22237
216.2
102856
97594
80
76
5.8
13353
10981
21098
228.5
92331
72
15401
13298
22152
201.9
109717
117064
85
91
8.1
14081
16955
30891
248.3
124411
96
14983
15087
25833
280
92261
105305
72
82
14.3
13978
14244
26143
220.9
118349
92
14523
14505
25623
216.9
118134
107898
92
84
11.2
16419
18682
29191
298.9
97661
76
13313
9191
17712
198.1
89408
120763
69
94
34.4
13999
14418
26423
173.7
152118
118
13096
11960
23430
216.5
108220
103774
84
80
4.9
14417
13268
23610
237.7
99328
77
12438
8472
17475
232.6
75127
83236
58
65
8.9
12519
9940
20370
223.0
91345
71
13661
6222
11685
174.3
67038
58119
52
45
9.8
14096
5074
9235
187.7
49200
38
12455
1670
3440
265.6
12951
14780
10
11
2.0
12791
2268
4549
273.9
16608
13
14394
624
1112
261.6
4251
4151
3
3
0.1
13181
465
905
223.4
4051
3
13006
7667
15124
273.9
55216
66147
51
61
14.3
13189
10370
20172
261.7
77079
71
13331
17500
33678
223.5
150685
138237
139
128
16.3
12774
14299
28718
228.3
125790
116
13454
8093
15432
189.7
81351
100007
75
92
24.4
16489
14468
22511
189.7
118664
110
12397
13939
28846
202.0
142802
124204
132
115
24.3
12966
11630
23012
217.9
105606
98
13703
10974
20546
216.5
94899
109301
88
101
18.8
13864
15549
28773
232.6
123702
114
15672
16480
26978
244.0
110564
108003
102
100
3.3
15719
17411
28417
269.5
105442
97
12626
9783
19878
282.5
70366
82685
65
76
16.1
13507
16521
31380
330.3
95004
88
15475
19586
32470
327.3
99207
105912
92
98
8.8
13164
12135
23650
210.0
112617
104
15165
9834
16636
264.5
62898
62847
58
58
0.1
15228
10776
18155
289.1
62797
58
[14C]-Cholesterol synthesis in rat hepatocytes
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Page A159 of A172
sample
55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107
compound concentration
Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M
Rosuvastatin 0.3 M Rosuvastatin 0.3 M
PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M
14C Label
14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid
14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid
14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C 14C
input
input
input
palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid palmitic acid 3H cholesterol 3H cholesterol 3H cholesterol
average input 3H cholesterol
3H-cholesterol (dpm) 15609 15687 14378 13730 15870 12832 13019 16614 16141 15873 17295 16841 10210 15012 15875 16131 16397 14452 15496 17004 13663 13951 14919 16126 15126 15117 15845 15353 16583 15158 14410 15961 16275 14927 14899 2959 14864 15988 14366 15716 13749 16009 14012 15284 14474 12042 14571 13686 13478 13285 12506 13476 14963 26014 25923 25028 25655
14C-cholesterol (dpm) 4123 4793 901 984 311 512 11827 19187 21002 19906 21050 16813 11716 18465 17804 20213 12748 11356 14299 17756 13984 16405 11557 12122 4914 5462 1201 1756 465 505 5450 5718 5596 5781 5367 1505 6675 4773 5602 8024 5977 5872 4048 9125 5125 5104 4478 4542 3476 2874 1940 1859 1145
14C-cholesterol corrected for recovery (dpm) 6777 7839 1608 1839 503 1024 23306 29628 33381 32173 31225 25612 29439 31556 28772 32147 19946 20159 23673 26790 26258 30168 19874 19285 8335 9270 1945 2934 719 855 9703 9191 8821 9936 9242 13049 11521 7659 10004 13098 11153 9410 7412 15317 9084 10874 7884 8514 6616 5550 3980 3539 1963
g protein
268.9 303.1 276.0 308.9 283.3 253.1 175.6 224.9 243.2 191.8 229.0 187.1 172.3 187.7 180.9 99.8 208.9 172.9 124.4 162.5 201.9 220.3 227.5 166.1 168.0 318.8 234.5 195.1 183.3 215.2 167.6 182.5 230.1 240.4 215.9 228.5 199.3 248.4 274.0 219.2 215.9 299.0 196.1 171.7 216.1 236.9 231.0 222.7 172.3 185.8 266.7 271.7 239.7
14C-cholesterol per mg protein (dpm/mg)
25201 25861 5825 5952 1775 4044 132722 131739 137258 167745 136354 136891 170860 168120 159051 322115 95480 116594 190300 164859 130053 136939 87357 116105 49611 29076 8292 15040 3925 3972 57894 50361 38336 41330 42805 57105 57807 30833 36511 59756 51657 31472 37795 89207 42036 45901 34131 38232 38401 29871 14922 13026 8190
Average14C-cholesterol per mg protein (dpm/mg)
25531 5889 2910 132231 152501 136623 168120 240583 106037 177579 133496 101731 39343 11666 3948 54127 39833 42805 44320 48134 41565 63501 43968 36182 34136 13974 8190
% of control Average % of control
23
24
24
5
5
6
2
3
4
85
84
84
88
97
107
87
87
87
109
108
107
102
154
206
61
68
74
122
113
105
83
85
87
56
65
74
32
25
19
5
7
10
3
3
3
82
76
71
54
56
58
60
71
81
82
63
44
52
68
84
73
59
44
53
90
126
59
62
65
48
51
54
54
48
42
21
20
18
12
12
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Appendix to TNO-report of 3M-02
Experiment 2
Final version
04-12-2006
Protein concentration determination
sample 1 2 3 4 5 6 7 8 9 10 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Measured protein conc (mg/ml)
191.5 204.6 218.1 230.3 234.8 221 267.6 215.2 187.7 214.2 222.9 233.8 235.8 266.6 250 260.3 232.8 241.9 246.9 269.7 211.4 195.2 155.9 154.1 123.5 172.1 156.8 231.8 139.1 114.9 147 194.3 256.2 220.1 126.6 143.5 221 217.1 158.6 179.4 235.8 224.9 175.8 241.8 196.3 204.6 206.6 200.8 240.8 242.9 190.5 191.5 150.6 154.1 142.6 140.9
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 792.2 896.8 911.6 965.6 803.8 913.4 1004.8 1020.6 949.4 1033.2 813.2 620.0 591.2 777.2 508.0 682.6 952.6 540.2 876.2 676.0 921.4 835.2 801.8 814.8 967.4 764.0 609.4 567.0
g protein per well 264.1 298.9 303.9 321.9 267.9 304.5 334.9 340.2 316.5 344.4 271.1 206.7 197.1 259.1 169.3 227.5 317.5 180.1 292.1 225.3 307.1 278.4 267.3 271.6 322.5 254.7 203.1 189.0
sample 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
Measured protein conc (mg/ml)
231.9 240.9 211.3 208.5 216.2 239.8 203.7 235.8 292.4 340.6 167.6 197.1 177.5 211.3 237.4 232.4 217.3 244.9 202.1 206.2 240 266.7 181.8 202.9 217.3 223.1 188.5 174.1 238.2 250 173.3 204.6 235.8 231.6 229.9 240 183.5 188.5 163.1 152 202.9 228.1 213.8 244.9 211.4 218.1 168.2 149.4 211.4 213 232.4 254.2 245.8 224 156.2 136.5
215.6 236.6 133.9 158.7 210.5 209.7 156.2 184.3
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 945.6 839.6 912.0 879.0 1266.0 729.4 777.6 939.6 924.4 816.6 1013.4 769.4 880.8 725.2 976.4 755.8 934.8 939.8 744.0 630.2 862.0 917.4 859.0 635.2 848.8 973.2 939.6 585.4 904.4 585.2 840.4 681.0
g protein per well 315.2 279.9 304.0 293.0 422.0 243.1 259.2 313.2 308.1 272.2 337.8 256.5 293.6 241.7 325.5 251.9 311.6 313.3 248.0 210.1 287.3 305.8 286.3 211.7 282.9 324.4 313.2 195.1 301.5 195.1 280.1 227.0
Page A160 of A172
Appendix to TNO-report of 3M-02
Final version
sample 63 64 63 64 63 64 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108
Measured protein conc (mg/ml)
252.5 225.7 153.6 142.5 228.2 210.5 228.1 204.6 220.6 244.2 133.9 150.2 220.6 224.8 234.9 236.6 247.5 230.7 137.3 156.4 206.2 194.7 150 175 152.7 142.8 141.9 140.1 230.3 233.8 237.3 211.6 184 146.9 118.4 200.1 180.4 235.6 162.6 132 211.6 252.4 168.8 170.6 222.3 242.6 257.7 247.1 223.1 208.9 178.7 215.1 179.6 194.7 168.8 142.9 188.5 222.3 196.5 180.4 226.7 240.9
182.2 228.4 240 165.2 142.8 272.7 243.5 201.8 167.1 213.4 236.5 254.2 219.6 153.6 122 232 281.5 207.2 227.6 208.1 207.2 192.9 161.7 281.6 218.7 145.5 151.8 162.6 137.4
dilution 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
average protein conc (mg/ml) 252 256 252 256 252 256 276 280 284 288 292 296 300 304 308 312 316 320 324 328 332 336 340 344 348 352 356 360 364 368 372 376 380 384 388 392 396 400 404 408 412 416 420 424 428 432
g protein per well 84.0 85.3 84.0 85.3 84.0 85.3 92.0 93.3 94.7 96.0 97.3 98.7 100.0 101.3 102.7 104.0 105.3 106.7 108.0 109.3 110.7 112.0 113.3 114.7 116.0 117.3 118.7 120.0 121.3 122.7 124.0 125.3 126.7 128.0 129.3 130.7 132.0 133.3 134.7 136.0 137.3 138.7 140.0 141.3 142.7 144.0
04-12-2006
Page A161 of A172
Page A162 of A172
sample compound concentration
1
control
2
control
3
control
4
control
5
control
6
control
7
control
8
control
9
control
13
PFBS 1 M
14
PFBS 1 M
15
PFBS 10 M
16
PFBS 10 M
17
PFBS 100 M
18
PFBS 100 M
19
PFHS 1 M
20
PFHS 1 M
21
PFHS 10 M
22
PFHS 10 M
23
PFHS 100 M
24
PFHS 100 M
25
PFOS 1 M
26
PFOS 1 M
27
PFOS 10 M
28
PFOS 10 M
29
PFOS 100 M
30
PFOS 100 M
31 Rosuvastatin 0.003 M
32 Rosuvastatin 0.003 M
33 Rosuvastatin 0.03 M
34 Rosuvastatin 0.03 M
35
Rosuvastatin 0.3 M
36
Rosuvastatin 0.3 M
37
PFBS 1 M
38
PFBS 1 M
39
PFBS 10 M
40
PFBS 10 M
41
PFBS 100 M
42
PFBS 100 M
43
PFHS 1 M
44
PFHS 1 M
45
PFHS 10 M
46
PFHS 10 M
47
PFHS 100 M
48
PFHS 100 M
49
PFOS 1 M
50
PFOS 1 M
51
PFOS 10 M
52
PFOS 10 M
53
PFOS 100 M
54
PFOS 100 M
14C Label
14C acetic acid 14C acetic acid 14C acetic acid
14C butyric acid 14C butyric acid 14C butyric acid
14C lauric acid 14C lauric acid 14C lauric acid
14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid
14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid
3H-cholesterol (dpm) 10176 15067 8013 7102 13239 16182 11285 9341 14585 10415 11106 13209 17775 11729 16244 15747 13331 15569 8199 15486 14084 15835 16929 15411 9454 15895 17054 18199 17403 16235 14014 15647 9439 17104 16962 16583 15691 14615 16380 16700 15447 15347 15658 16797 16476 14120 13621 16279 16347 15609 16385
14C-cholesterol (dpm) 13013 29921 20565 3628 7313 13946 19393 14887 19635 15531 22304 31195 41007 24081 42002 34491 30717 28923 21682 25911 36299 30330 46461 37008 20098 26782 29629 16110 11966 3127 1836 499 439 8190 23749 11608 16193 17212 17173 12743 20907 18404 22910 19524 25363 11804 21880 28289 17820 18990 28115
14C-cholesterol corrected for recovery (dpm) 34516 53601 69272 13788 14910 23262 46384 43017 36337 40250 54206 63744 62269 55416 69791 59120 62193 50143 71378 45162 69565 51699 74077 64817 57380 45479 46894 23893 18559 5199 3536 861 1255 12924 37791 18894 27855 31788 28298 20596 36532 32368 39492 31373 41550 24616 43357 46904 29423 32838 46314
g protein
264.1 298.9 303.9 321.9 267.9 304.5 334.9 340.2 316.5 271.1 206.7 197.1 259.1 169.3 227.5 317.5 180.1 292.1 225.3 307.1 278.4 267.3 271.6 322.5 254.7 203.1 189.0 315.2 279.9 304.0 293.0 422.0 243.1 259.2 313.2 308.1 272.2 337.8 256.5 293.6 241.7 325.5 251.9 311.6 313.3 248.0 210.1 287.3 305.8 286.3 211.7
14C-cholesterol per mg protein (dpm/mg)
130711 179328 227969 42839 55647 76402 138487 126446 114821 148487 262288 323464 240359 327262 306730 186184 345324 171682 316765 147042 249876 193434 272742 201004 225315 223922 248115 75803 66313 17101 12069
2040 5163 49863 120662 61317 102332 94102 110338 70149 151125 99450 156757 100685 132635 99258 206398 163241 96218 114684 218739
Average14C-cholesterol per mg protein (dpm/mg)
179336
58296
126585
205388 281912 316996 265754 244224 198459 233088 213159 236019 71058 14585
3601 85262 81825 102220 110637 128104 116660 152828 129729 166712
% of control Average % of control
73
100
100
127
73
100
95
131
83
115
146
180
157
134
182
177
171
104
148
193
96
136
177
82
111
139
108
130
152
112
119
126
125
132
138
42
40
37
10
8
7
1
2
3
86
146
207
105
140
176
161
175
189
120
190
259
171
220
269
173
200
228
170
262
354
280
223
165
197
286
375
SD 27.1
29.1
44.9 32.8 8.1 62.7 57.2 40.5 31.3 9.6 9.5 3.7 2.0 1.2 85.9 49.8 19.7 98.2 69.5 38.8 130.0 81.3 126.2
[14C]-Cholesterol synthesis in rat hepatocytes
04-12-2006
Final version
Appendix to TNO-report of 3M-02
sample compound concentration
14C Label
3H-cholesterol 14C-cholesterol 14C-cholesterol corrected g protein
14C-cholesterol
Average14C-cholesterol % of control Average
SD
(dpm)
(dpm)
for recovery (dpm)
per mg protein (dpm/mg) per mg protein (dpm/mg)
% of control
Appendix to TNO-report of 3M-02
55 Rosuvastatin 0.003 M
14C butyric acid
16224
4328
7200
282.9
25449
27019
44
46
3.8
56 Rosuvastatin 0.003 M
14C butyric acid
16103
5533
9274
324.4
28589
49
57 Rosuvastatin 0.03 M 58 Rosuvastatin 0.03 M
14C butyric acid 14C butyric acid
16808 17593
975 1781
1566 2732
313.2 195.1
4999 14003
9501
9
16
10.9
24
59
Rosuvastatin 0.3 M
14C butyric acid
14906
452
818
301.5
2715
2604
5
4
0.3
60
Rosuvastatin 0.3 M
14C butyric acid
15758
284
486
195.1
2494
4
61
PFBS 1 M
14C lauric acid
14491
19019
38645
280.1
137952
165768
109
131
31.1
62
PFBS 1 M
14C lauric acid
17266
28110
43943
227.0
193583
153
63
PFBS 10 M
14C lauric acid
16567
21487
35007
318.8
109809
129179
87
102
21.6
64
PFBS 10 M
14C lauric acid
17868
19412
29324
197.4
148550
117
65
PFBS 100 M
14C lauric acid
17520
21648
33351
292.5
114033
127014
90
100
14.5
66
PFBS 100 M
14C lauric acid
19364
28972
40384
288.5
139995
111
67
PFHS 1 M
14C lauric acid
16638
26282
42637
309.9
137582
168120
109
150
58.5
68
PFHS 1 M
14C lauric acid
17741
30159
45884
189.4
242261
191
69
PFHS 10 M
14C lauric acid
17115
25762
40628
296.9
136826
130644
108
103
6.9
70
PFHS 10 M
14C lauric acid
16449
23842
39123
314.3
124462
98
71
PFHS 100 M
14C lauric acid
15800
21356
36483
318.8
114438
169881
90
134
61.9
72
PFHS 100 M
14C lauric acid
16793
27449
44119
195.8
225325
178
73
PFOS 1 M
14C lauric acid
16050
18811
31635
267.3
118363
150205
94
119
35.6
74
PFOS 1 M
14C lauric acid
17035
24894
39444
216.7
182048
144
75
PFOS 10 M
14C lauric acid
16660
16687
27035
197.0
137234
157048
108
124
22.1
76
PFOS 10 M
14C lauric acid
15663
19295
33250
188.0
176863
140
77
PFOS 100 M
14C lauric acid
16046
27259
45853
309.4
148200
157868
117
125
10.8
78
PFOS 100 M
14C lauric acid
13213
24544
50138
299.3
167537
132
Final version
79 Rosuvastatin 0.003 M
14C lauric acid
13776
6081
11915
220.6
54010
44273
43
35
10.9
80 Rosuvastatin 0.003 M
14C lauric acid
12989
3529
7333
212.3
34537
27
81 Rosuvastatin 0.03 M 82 Rosuvastatin 0.03 M
14C lauric acid 14C lauric acid
15098 11184
1767 2114
3159 5102
277.3 196.4
11390 25977
18684
9
15
8.1
21
83
Rosuvastatin 0.3 M
14C lauric acid
13447
566
1136
309.3
3673
3183
3
3
0.5
84
Rosuvastatin 0.3 M
14C lauric acid
14569
329
610
226.3
2694
2
input 3H cholesterol
26701
input 3H cholesterol input 3H cholesterol
27378 26895
average input 3H cholesterol 26991.33333
Page A163 of A172
04-12-2006
Appendix to TNO-report of 3M-02
Experiment 3
Final version
Protein concentration determination
sample
1 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55
Measured
protein conc (mg/ml)
246.35 298.46 229.35 266.32 232.70 299.74 186.54 154.56 143.00 169.33 153.58 301.02 220.50 370.42 222.70 224.91 171.32 272.34 180.40 240.62 227.12 209.66 295.91 248.65 295.91 331.42 268.72 222.71 282.13 236.08 172.33 263.93 175.34 171.32 247.50 249.81 393.62 348.03 289.60 153.58 227.12 209.66 317.98 272.34 211.81 258.00 195.88 304.88 389.03 352.27 258.00 279.67 363.75 295.91
dilution
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
protein conc
(mg/ml)
985.4 1193.8 917.4 1065.3 930.8 1199.0 746.2 618.2 572.0 677.3 614.3 1204.1 882.0 1481.7 890.8 899.6 685.3 1089.4 721.6 962.5 908.5 838.6 1183.6 994.6 1183.6 1325.7 1074.9 890.8 1128.5 944.3 689.3 1055.7 701.4 685.3 990.0 999.2 1574.5 1392.1 1158.4 614.3 908.5 838.6 1271.9 1089.4 847.2 1032.0 783.5 1219.5 1556.1 1409.1 1032.0 1118.7 1455.0 1183.6
g protein
per well
328.5 397.9 305.8 355.1 310.3 399.7 248.7 206.1 190.7 225.8 204.8 401.4 294.0 493.9 296.9 299.9 228.4 363.1 240.5 320.8 302.8 279.5 394.5 331.5 394.5 441.9 358.3 296.9 376.2 314.8 229.8 351.9 233.8 228.4 330.0 333.1 524.8 464.0 386.1 204.8 302.8 279.5 424.0 363.1 282.4 344.0 261.2 406.5 518.7 469.7 344.0 372.9 485.0 394.5
04-12-2006
Page A164 of A172
Appendix to TNO-report of 3M-02
Final version
sample
56 57 58 59 60 61
62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108
Measured protein conc (mg/ml)
356.51 319.45 356.51 319.45 226.01 241.05
333.17 251.23 297.09 232.80 268.15 294.15 222.82 295.13 244.74 237.38 207.61 324.00 253.09 241.05 283.46 267.20 275.77 347.64 216.53 368.75 219.00 329.09 221.92 285.39 348.30 312.93 212.95 288.30 281.53 305.97 245.66 274.81 289.28 254.03 230.98 288.30 332.15 294.15 219.22 335.22 252.16 297.09 247.52 280.57 250.30 296.11 217.43
dilution
4 4 4 4 4 4
4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
protein conc (mg/ml)
1426.0 1277.8 1426.0 1277.8 904.0 964.2
1332.7 1004.9 1188.4 931.2 1072.6 1176.6 891.3 1180.5 979.0 949.5 830.4 1296.0 1012.4 964.2 1133.8 1068.8 1103.1 1390.6 866.1 1475.0 876.0 1316.4 887.7 1141.6 1393.2 1251.7 851.8 1153.2 1126.1 1223.9 982.6 1099.2 1157.1 1016.1 923.9 1153.2 1328.6 1176.6 876.9 1340.9 1008.6 1188.4 990.1 1122.3 1001.2 1184.4 869.7
g protein per well
475.3 425.9 475.3 425.9 301.3 321.4
444.2 335.0 396.1 310.4 357.5 392.2 297.1 393.5 326.3 316.5 276.8 432.0 337.5 321.4 377.9 356.3 367.7 463.5 288.7 491.7 292.0 438.8 295.9 380.5 464.4 417.2 283.9 384.4 375.4 408.0 327.5 366.4 385.7 338.7 308.0 384.4 442.9 392.2 292.3 447.0 336.2 396.1 330.0 374.1 333.7 394.8 289.9
04-12-2006
Page A165 of A172
Page A166 of A172
3H cholesterol mix 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
sample
1 3 4 5 6 7 8 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
compound concentration
control control control control control control control control control PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M PFBS 1 M PFBS 1 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M
14C Label
14C acetic acid 14C acetic acid 14C butyric acid 14C butyric acid 14C butyric acid 14C lauric acid 14C lauric acid 14C palmitic acid 14C palmitic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid
3H-cholesterol (dpm) 12507 19023 16324 15491 14301 15647 14301 16834 17733 17724 15643 13898 17481 17149 18303 17772 16907 17592 16152 18689 17098 17841 19413 17762 14828 16243 15814 15332 14969 14770 14215 16761 16727 15968 14142 16118 16293 19457 18922 17479 17443 16118 18498 17002 17226 16924 15074 17062 15031 18507 14681 15247 15905 17593 13507 18268
14C-cholesterol (dpm) 44451 99078 18935 15714 17937 40817 32511 5000 4789 22246 21849 22496 26287 23557 22881 35195 39844 23683 24871 27769 22118 27624 43559 25533 24114 22967 26323 2953 3029 574 626 257 248 5934 11778 13512 9931 7769 6439 14976 7993 9922 8585 10438 7974 8198 12993 18014 12106 9530 930 687 276 212 94 120
14C-cholesterol corrected for recovery (dpm) 91111 133519 29736 26005 32153 66874 58279 7614 6923 32176 35806 41495 38550 35215 32048 50768 60414 34512 39474 38091 33162 39693 57521 36851 41690 36248 42672 4938 5187 996 1129 393 380 9527 21350 21491 15626 10236 8724 21965 11747 15781 11898 15738 11867 12418 22097 27066 20647 13201 1624 1155 445 309 178 168
g protein
328.5 397.9 305.8 355.1 310.3 399.7 248.7 225.8 204.8 401.4 294.0 493.9 296.9 299.9 228.4 363.1 240.5 320.8 302.8 279.5 394.5 331.5 394.5 441.9 358.3 296.9 376.2 314.8 229.8 351.9 233.8 228.4 330.0 333.1 524.8 464.0 204.8 302.8 279.5 424.0 363.1 282.4 344.0 261.2 406.5 518.7 469.7 344.0 372.9 485.0 394.5 475.3 425.9 475.3 425.9 301.3
14C-cholesterol per mg protein (dpm/mg)
277384 335520 97240 73233 103632 167329 234314 33725 33809 80168 121789 84018 129826 117430 140298 139810 251169 107571 130352 136259 84052 119725 145791 83394 116357 122069 113436 15686 22576
2831 4829 1721 1152 28602 40681 46312 76307 33802 31206 51807 32351 55879 34586 60261 29192 23940 47045 78680 55370 27218 4116 2430 1044 650 419 559
Average14C-cholesterol per mg protein (dpm/mg)
306452 91368
200821 33767 100979 106922 128864 195490 118962 110155 132758 99876 117752 19131 3830 1436 34641 46312 55054 41506 44115 47423 26566 62863 41294 3273
847 489
% of control Average SD % of control
90.5
100.0 13.4
109.5
106.4
100.0 17.5
80.2
113.4
83.3
100.0 23.6
116.7
99.9
100.0 0.18
100.1
26.2
33.0
9.6
39.7
27.4
34.9 10.6
42.4
38.3
42.1
5.3
45.8
45.6
63.8 25.7
82.0
35.1
38.8
5.3
42.5
44.5
35.9 12.0
27.4
39.1
43.3
6.0
47.6
27.2
32.6
7.6
38.0
39.8
38.4
2.0
37.0
5.1
6.2
1.6
7.4
0.9
1.2
0.5
1.6
0.6
0.5
0.1
0.4
31.3
37.9
9.3
44.5
50.7
50.7
83.5
60.3 32.9
37.0
34.2
45.4 15.9
56.7
35.4
48.3 18.2
61.2
37.9
51.9 19.9
66.0
32.0
29.1
4.1
26.2
51.5
68.8 24.5
86.1
60.6
45.2 21.8
29.8
4.5
3.6
1.3
2.7
1.1
0.9
0.3
0.7
0.5
0.5
0.1
0.6
[14C]-Cholesterol synthesis in rat hepatocytes
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Page A167 of A172
3H cholesterol mix 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
sample
61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108
compound concentration
PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M
14C Label
14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C lauric acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid
input 3H cholesterol input 3H cholesterol input 3H cholesterol average input 3H cholesterol
3H-cholesterol (dpm) 18064 16312 16632 16430 17140 16929 17947 17680 17511 18652 17842 17390 12194 17125 16554 16019 15880 17209 15981 14109 17448 16191 15558 16555 17040 15827 16659 15285 17337 14572 14708 15562 14849 16477 15761 16923 19055 16210 17729 16090 17458 12554 17205 16635 17350 13361 15847 mix 1 25860 25671 25376 25635.7
14C-cholesterol (dpm) 35197 33843 31024 42519 24245 22983 34462 35488 31561 34500 20913 30612 39952 49945 29563 42312 22742 31390 3218 3171 754 686 261 4312 3219 3366 4602 2953 3284 2399 3322 3238 3857 2952 3697 3859 4608 4074 5568 2981 3072 711 986 534 544 313 359 mix 2 24418 25195 24950 24854.3
14C-cholesterol corrected for recovery (dpm) 49950 53187 47819 66342 36262 34803 49226 51457 46205 47417 30048 45127 81432 72488 44386 65649 35594 45335 5005 5586 1074 1053 417 6474 4695 5286 6866 4802 4708 4092 5614 5171 6456 4453 5830 5668 6010 6247 7806 4605 4373 1408 1424 798 779 582 563
g protein
321.4 444.2 335.0 396.1 310.4 357.5 392.2 297.1 393.5 326.3 316.5 276.8 432.0 337.5 321.4 377.9 356.3 367.7 463.5 288.7 491.7 292.0 438.8 380.5 464.4 417.2 283.9 384.4 375.4 408.0 327.5 366.4 385.7 338.7 308.0 384.4 442.9 392.2 292.3 447.0 336.2 396.1 330.0 374.1 333.7 394.8 289.9
14C-cholesterol per mg protein (dpm/mg)
155414 119730 142754 167480 116825 97343 125512 173201 117417 145310 94937 163023 188500 214808 138103 173700 99909 123297 10797 19348
2185 3606 950 17013 10110 12669 24182 12492 12542 10030 17139 14114 16738 13147 18930 14744 13572 15927 26705 10302 13008 3554 4316 2133 2335 1475 1942
Average14C-cholesterol per mg protein (dpm/mg)
137572 155117 107084 168120 131364 128980 201654 155901 111603 15073
2895 1050 13562 18425 42805 13584 15426 16038 14158 21316 11655 3935 2234 8190
% of control Average SD % of control
77.4
68.5 12.6
59.6
71.1
77.2
8.7
83.4
58.2
53.3
6.9
48.5
62.5
74.4 16.8
86.2
58.5
65.4
9.8
72.4
47.3
64.2 24.0
81.2
93.9
100.4 9.3
107.0
68.8
77.6 12.5
86.5
49.8
55.6
8.2
61.4
5.4
7.5
3.0
9.6
1.1
1.4
0.5
1.8
0.5
0.5
0.1
50.4
50.4
37.5
54.6 24.1
71.6
37.0
37.1
0.1
37.1
29.7
40.2 14.9
50.8
41.8
45.7
5.5
49.6
38.9
47.5 12.1
56.1
43.7
41.9
2.5
40.2
47.2
63.1 22.6
79.1
30.5
34.5
5.7
38.5
10.5
11.7
1.6
12.8
6.3
6.6
0.4
6.9
4.4
5.1
1.0
5.8
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Appendix to TNO-report of 3M-02
Experiment 4
Final version
04-12-2006
Protein concentration determination
sample
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56
Measured protein conc (mg/ml)
well 1
well 2
207.81
223.77
214.31
224.72
189.73
202.31
205.97
230.5
234.4
252.34
221.86
205.97
218.07
224.72
225.68
225.68
214.31
223.77
210.58
214.31
210.58
220.91
199.58
205.97
212.44
214.31
241.29
245.28
230.5
226.54
234.4
239.31
272.05
262.61
292.63
272.24
254.37
242.28
231.47
237.34
269.94
274.18
272.05
253.35
253.35
250.31
242.28
221.86
202.31
210.58
241.29
242.28
245.28
239.31
230.5
235.37
245.28
233.42
218.07
209.66
250.31
240.3
253.35
242.28
244.28
249.3
233.42
237.34
215.24
204.14
227.6
210.58
249.52
228.83
256.56
246.52
277.1
284.44
248.52
238.6
251.52
254.54
266.75
258.59
238.6
246.52
265.73
254.54
251.52
253.53
242.55
245.53
237.62
229.8
212.56
208.8
211.62
203.19
206.92
209.73
194.87
192.13
206.92
210.68
222.08
234.67
177.7
186.68
214.45
228.83
220.17
214.45
dilution
5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5
protein conc
(mg/ml)
1079.0 1097.6 980.1 1091.2 1216.9 1069.6 1107.0 1128.4 1095.2 1062.2 1078.7 1013.9 1066.9 1216.4 1142.6 1184.3 1336.7 1412.2 1241.6 1172.0 1360.3 1313.5 1259.2 1160.4 1032.2 1208.9 1211.5 1164.7 1196.8 1069.3 1226.5 1239.1 1234.0 1176.9 1048.5 1095.5 1195.9 1257.7 1403.9 1217.8 1265.2 1313.4 1212.8 1300.7 1262.6 1220.2 1168.6 1053.4 1037.0 1041.6 967.5 1044.0 1141.9 911.0 1108.2 1086.6
g protein
per well
359.7 365.9 326.7 363.7 405.6 356.5 369.0 376.1 365.1 354.1 359.6 338.0 355.6 405.5 380.9 394.8 445.6 470.7 413.9 390.7 453.4 437.8 419.7 386.8 344.1 403.0 403.8 388.2 398.9 356.4 408.8 413.0 411.3 392.3 349.5 365.2 398.6 419.2 468.0 405.9 421.7 437.8 404.3 433.6 420.9 406.7 389.5 351.1 345.7 347.2 322.5 348.0 380.6 303.7 369.4 362.2
Page A168 of A172
Appendix to TNO-report of 3M-02
Final version
04-12-2006
sample
57 58 59 60 61
62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 91 92 94 95 96 97 98 100 101 103 104 105 106 107 109 110 112
113
114 115 116 118 119
Measured protein conc (mg/ml)
well 1
well 2
224.96
223.04
219.21
218.26
213.51
204.12
199.48
205.99
210.68
204.12
186.68
193.04
160.17
161.9
209.73
203.19
205.99
217.3
229.8
225.93
237.62
246.52
222.08
234.67
261.64
263.68
259.6
235.65
239.59
255.55
278.14
285.49
212.61
215.22
232.92
229.35
225.79
240.13
231.13
233.82
233.82
241.03
225.79
239.22
241.03
238.32
239.22
232.03
217.85
221.37
224.02
234.72
220.49
225.79
283
287.81
208.27
190.32
204.81
214.35
203.09
217.85
218.73
214.35
216.10
212.61
205.67
203.95
224.02
217.85
218.73
211.74
233.82
224.02
226.68
218.73
242.85
235.62
252.91
239.22
242.85
245.58
218.73
209.13
255.68
256.60
242.85
245.58
248.32
237.42
229.35
242.85
251.99
243.76
245.58
259.39
231.13
217.85
251.07
255.68
272.51
291.69
219.61
216.10
243.74
246.67
255.52
269.48
267.47
271.50
234.05
235.01
dilution
5 5 5 5 5
5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5
5
5 5 5 5 5
protein conc
(mg/ml)
1120.0 1093.7 1044.1 1013.7 1037.0
949.3 805.2 1032.3 1058.2 1139.3 1210.4 1141.9 1313.3 1238.1 1237.9 1409.1 1069.6 1155.7 1164.8 1162.4 1187.1 1162.5 1198.4 1178.1 1098.1 1146.9 1115.7 1427.0 996.5 1047.9 1052.4 1082.7 1071.8 1024.1 1104.7 1076.2 1144.6 1113.5 1196.2 1230.3 1221.1 1069.7 1280.7 1221.1 1214.4 1180.5 1239.4 1262.4 1122.5 1266.9
1410.5
1089.3 1226.0 1312.5 1347.4 1172.7
g protein
per well
373.3 364.6 348.0 337.9 345.7
316.4 268.4 344.1 352.7 379.8 403.5 380.6 437.8 412.7 412.6 469.7 356.5 385.2 388.3 387.5 395.7 387.5 399.5 392.7 366.0 382.3 371.9 475.7 332.2 349.3 350.8 360.9 357.3 341.4 368.2 358.7 381.5 371.2 398.7 410.1 407.0 356.6 426.9 407.0 404.8 393.5 413.1 420.8 374.2 422.3
470.2
363.1 408.7 437.5 449.1 390.9
Page A169 of A172
Page A170 of A172
3H cholesterol mix 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
sample
1 2 3 4 5 7 8 9 10 11 12 13 15 17 18 19 20 22 23 24 25 26 27 28 30 31 32 33 34 35 36 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 56 57 58
compound concentration
control control control control control control control control control control control PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M PFBS 1 M PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M
14C Label
14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C butyric acid 14C butyric acid 14C butyric acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C palmitic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid
14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid 14C acetic acid
14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid 14C butyric acid
3H-cholesterol 14C-cholesterol 14C-cholesterol corrected g protein
14C-cholesterol
% of control Average SD
(dpm)
(dpm)
for recovery (dpm)
per mg protein (dpm/mg)
% of control
11557
1815
3220
359.7
8953
98.5
100.0
12.7
13921
1877
2765
365.9
7556
83.2
12789
2094
3357
326.7
10276
113.1
14330
2430
3477
363.7
9559
105.2
14143
2257
3272
405.6
8067
64.9
100.0
33.3
12409
2888
4772
369.0
12932
104.0
13395
4007
6134
376.1
16307
131.1
12206
1142
1918
365.1
5255
76.4
100.0
16.2
13316
1694
2608
354.1
7367
107.2
12616
1574
2558
359.6
7114
103.5
13307
1703
2624
338.0
7764
112.9
14019
2569
3757
355.6
10566
116.3
90.0
37.2
14018
1506
2203
380.9
5784
63.7
13123
1714
2678
445.6
6011
66.2
67.3
1.6
12859
1837
2929
470.7
6223
68.5
12350
1427
2369
413.9
5724
63.0
93.7
43.4
12583
2709
4414
390.7
11299
124.4
12002
1811
3094
437.8
7066
77.8
91.8
21.4
12074
1822
3094
419.7
7372
81.1
10469
2089
4091
386.8
10578
116.4
12178
2935
4942
344.1
14362
158.1
154.3
20.2
13070
3091
4849
403.0
12033
132.4
11021
3398
6322
403.8
15655
172.3
12494
2059
3379
388.2
8704
95.8
100.0
5.9
11696
1924
3373
356.4
9463
104.1
12079
3650
6196
408.8
15155
166.8
139.9
24.2
12015
2929
4998
413.0
12102
133.2
12687
2772
4480
411.3
10892
119.9
12477
2383
3916
392.3
9982
109.9
113.9
8.6
12560
2407
3929
349.5
11243
123.7
10462
1828
3583
365.2
9811
108.0
10416
1647
3242
419.2
7733
85.1
89.0
5.4
9390
1807
3946
468.0
8432
92.8
9534
81
171
405.9
420
4.6
3.8
1.1
11731
95
163
421.7
386
4.2
12187
61
100
437.8
230
2.5
11494
36
63
404.3
156
1.7
1.4
0.5
12998
42
65
433.6
150
1.6
10852
18
33
420.9
79
0.9
9480
7
15
406.7
36
0.4
0.6
0.1
8849
10
23
389.5
58
0.6
11450
12
21
351.1
60
0.7
9541
1216
2559
345.7
7402
59.5
92.0
36.8
11879
2158
3647
347.2
10504
84.5
12114
3192
5290
322.5
16403
131.9
11350
998
1765
348.0
5073
40.8
52.2
10.1
11456
1499
2627
380.6
6902
55.5
9935
1125
2273
303.7
7487
60.2
11036
4223
7682
362.2
21211
170.6
118.7
73.4
9818
1517
3102
373.3
8309
66.8
12175
1686
2780
364.6
7626
61.3
124.5
62.2
[14C]-Cholesterol synthesis in rat hepatocytes
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Page A171 of A172
3H cholesterol mix 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
sample
59 60 61 62 64 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 85 86 87 88 89 91 92 94 95 96 97 98 100 101 103 104 105 106 107 109 110 112 113 114 115 116 118 119
compound concentration
PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M PFBS 1 M PFBS 1 M PFBS 1 M PFBS 10 M PFBS 10 M PFBS 100 M PFBS 100 M PFHS 1 M PFHS 1 M PFHS 1 M PFHS 10 M PFHS 10 M PFHS 100 M PFHS 100 M PFOS 1 M PFOS 1 M PFOS 1 M PFOS 10 M PFOS 10 M PFOS 100 M PFOS 100 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.003 M Rosuvastatin 0.03 M Rosuvastatin 0.03 M Rosuvastatin 0.3 M Rosuvastatin 0.3 M
14C Label
3H-cholesterol 14C-cholesterol 14C-cholesterol corrected g protein
14C-cholesterol
% of control Average SD
(dpm)
(dpm)
for recovery (dpm)
per mg protein (dpm/mg)
% of control
14C butyric acid
11671
3180
5470
348.0
15718
126.4
14C butyric acid
11805
4589
7804
337.9
23097
185.7
14C butyric acid
11641
3479
6000
345.7
17357
139.6
175.7
64.8
14C butyric acid
11648
4837
8337
316.4
26346
211.9
14C butyric acid 14C butyric acid
11906 12251
2793 3204
4710 5250
344.1 379.8
13687 13825
110.1 111.2
110.6
0.8
14C butyric acid
12599
1926
3069
403.5
7607
61.2
71.1
15.9
14C butyric acid
10509
2215
4231
380.6
11117
89.4
14C butyric acid
12593
2141
3413
437.8
7797
62.7
14C butyric acid
12245
2079
3409
412.7
8259
66.4
73.4
8.4
14C butyric acid
11423
2076
3649
412.6
8843
71.1
14C butyric acid
12013
2892
4833
469.7
10290
82.7
14C butyric acid
11885
2655
4485
356.5
12579
101.2
92.1
10.1
14C butyric acid 14C butyric acid
11791 11061
2641 2162
4497
385.2
11673
93.9
3924
388.3
10107
81.3
14C butyric acid
12385
137
222
387.5
573
4.6
4.8
0.7
14C butyric acid
12579
127
203
395.7
512
4.1
14C butyric acid
12107
162
269
387.5
693
5.6
14C butyric acid
12774
35
55
399.5
138
1.1
1.3
0.2
14C butyric acid
11196
33
59
392.7
151
1.2
14C butyric acid
12355
43
70
366.0
191
1.5
14C butyric acid
13007
30
46
382.3
121
1.0
1.2
0.4
14C butyric acid
12240
43
71
371.9
190
1.5
14C palmitic acid
14199
978
1383
332.2
4163
60.6
56.3
7.1
14C palmitic acid
12961
745
1154
349.3
3304
48.1
14C palmitic acid
13307
963
1453
350.8
4142
60.2
14C palmitic acid
13107
863
1322
360.9
3663
53.3
40.8
17.6
14C palmitic acid
12361
429
697
357.3
1950
28.4
14C palmitic acid
14351
1098
1536
341.4
4500
65.5
62.4
4.3
14C palmitic acid
12742
954
1503
368.2
4082
59.4
14C palmitic acid
13653
1536
2259
358.7
6296
91.6
84.3
7.1
14C palmitic acid
12703
1392
2200
381.5
5766
83.9
14C palmitic acid
12038
1184
1975
371.2
5320
77.4
14C palmitic acid
13137
1031
1576
398.7
3952
57.5
58.2
1.1
14C palmitic acid
11960
991
1663
410.1
4056
59.0
14C palmitic acid
11398
1551
2732
407.0
6712
97.6
82.3
21.7
14C palmitic acid
14337
1171
1640
356.6
4599
66.9
14C palmitic acid
11689
851
1462
426.9
3424
49.8
57.4
8.1
14C palmitic acid
13496
1062
1580
407.0
3881
56.5
14C palmitic acid
13069
1193
1833
404.8
4527
65.9
14C palmitic acid
13862
1377
1994
393.5
5068
73.7
71.1
3.7
14C palmitic acid
14202
1377
1947
413.1
4712
68.5
14C palmitic acid
9771
742
1525
420.8
3623
52.7
70.7
25.5
14C palmitic acid
8624
980
2281
374.2
6097
88.7
14C palmitic acid
6247
152
488
422.3
1157
16.8
11.7
4.4
14C palmitic acid
8729
122
281
470.2
597
8.7
14C palmitic acid
10667
128
241
363.1
663
9.7
14C palmitic acid
6797
127
375
408.7
918
13.4
14.3
1.3
14C palmitic acid
4697
107
457
437.5
1045
15.2
14C palmitic acid
9054
141
313
449.1
696
10.1
15.1
7.0
14C palmitic acid
4674
125
537
390.9
1374
20.0
mix 1
mix 2
input 3H cholesterol
21387
21723
input 3H cholesterol input 3H cholesterol
20596 19529
17423 21082
average input 3H cholesterol
20504
20076
04-12-2006
Final version
Appendix to TNO-report of 3M-02
Appendix to TNO-report of 3M-02
Final version
04-12-2006
Average [14C]-Cholesterol synthesis in rat hepatocytes (% of control)
Concentration:
experiment 1 2 3 4
AVERAGE SD
experiment 1 2 3 4
AVERAGE SD
experiment 1
1 M 10 M 100 M 0.003 M 14C acetate incorporation, n=4, PFBS
85.0
82
76
115
157
177
33
34.9
42.1
90
67.3
93.7
80.8 85.3
97.2
34.4 51.7
57.3
14C acetate incorporation, n=4, PFHS
91
82
84
148
136
111
63.8 38.8
35.9
91.8 154.3
100
98.7 102.8
82.7
35.4 52.5
33.1
14C acetate incorporation, n=4, PFOS
76
84
65
2 3 4 AVERAGE SD
experiment 1 2 3 4
AVERAGE SD
130
119
132
43.3 32.6
38.4
139.9 113.9
89
97.3 87.4
81.1
45.7 39.6
39.7
14C acetate incorporation, n=4, Rosuvastatin
45
40.0
6.2
3.8
23.8
21.8
0.03 M
11 8 1 1 5.4 4.9
0.3 M
3 2 0.5 0.6 1.5 1.2
Concentration:
experiment 1 2 3 4
AVERAGE SD
experiment 1 2 3 4
AVERAGE SD
experiment 1 2 3 4
AVERAGE SD
experiment
1 2 3 4 AVERAGE SD
1 M 10 M 100 M 0.003 M 14C butyrate incorporation, n=4, PFBS
61.0 128
92
24
146
140
175
37.9 50.7
60.3
92
52.2
118.7
84.2 92.7
111.5
46.8 47.9
48.6
14C butyrate incorporation, n=4, PFHS
115
101
100
190
220
200
45.4 48.3
51.9
124.5 175.7
110.6
118.7 136.3
115.6
59.2 76.5
61.8
14C butyrate incorporation, n=4, PFOS
76
98
58
262
223
286
29.1 68.8
45.5
71.1 73.4
92.1
109.6 115.8
120.4
103.8 72.6
112.1
14C butyrate incorporation, n=4, rosuvastatin
24
46.0
3.7
4.8
19.6
19.9
0.03 M 5
5 16 1 1 5.8 7.0
0.3 M 3.0
3 4 0.5 1.2 2.2 1.6
Concentration: experiment 1 2 3 AVERAGE SD
1 2 3 AVERAGE SD
1 2 3 AVERAGE SD
1 2 3 AVERAGE SD
1 M 10 M 100 M 0.003 M 14C laurate incorporation, n=3, PFBS
84.0
97
87
131
102
100
68.5 77.2
53.3
94.5 92.1
80.1
32.5 13.1
24.1
14C laurate incorporation, n=3, PFHS
108
102
68
150
103
134
74.4 65.4
64.2
110.8 90.1
88.7
37.9 21.4
39.2
14C laurate incorporation, n=3, PFOS
113
85
65
119
124
125
100.4 77.6
55.6
110.8 95.5
81.9
9.5
24.9
37.6
14C laurate incorporation, n=3, rosuvastatin
25
35.0
7.5
22.5
13.9
0.03 M
7 15 1 7.8 6.8
0.3 M
3 3 0.5 2.2 1.4
Concentration: experiment 1 3 4 AVERAGE SD
1 3 4 AVERAGE SD
1 3 4 AVERAGE SD
1 3 4 AVERAGE SD
1 M 10 M 100 M 0.003 M 14C palmitate incorporation, n=3, PFBS
76.0
56
71
50.4 54.6
37.1
56.3 40.8
62.4
60.9 50.5
56.8
13.4
8.4
17.6
14C palmitate incorporation, n=3, PFHS
63
68
59
40.2 45.7
47.5
84.3 58.2
82.3
62.5 57.3
62.9
22.1 11.2
17.7
14C palmitate incorporation, n=3, PFOS
90
62
51
41.6 63.1
34.5
57.4 71.1
70.7
63.0 65.4
52.1
24.7
5.0
18.1
14C palmitate incorporation, n=3, rosuvastatin
48
11.7 11.7
23.8 21.0
0.03 M
20 7 14 13.8 6.5
0.3 M
12 5 15.1 10.7 5.2
Page A172 of A172