Document Jrbgp0ZrRnoaj1ayvYyMvw4Yv
AR 226 -0917
TOXICITY TO AQUATIC PLANTS
EST SUBSTANCE
Identity: ArefmeirxrteudretocoanstaPiFnOinSg,peFrCf-i9u5o,rooorctaasneasculofmopnoantee,ntwhoifcFhX-m3a1y04a.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3).
RiseFmXa-r3k1s0:4,
6%T.heC3urMrepnrtodiuncftoiromnatliotonniunmdbiecartwesaistinsoat
noted. The test sample mixtureof0.48% PFOS,
114..4049%%sodideituhmyloecnteylglsuylcfoalteb,ut4yl%etinhheirb,it5or9,%2w.a0t%erp,ol1y7o.x6y%eetthhyylelneeneglyco,
monooctylphenyl ether, 1.25% propane sultone foamer, 0.03% sodium lauryl
sulfate, and 0.1% benzotriazole.
Tchhaerfaocltleorwiiznegd scounmcmeantrryataipopnlsioesftiompaurmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical component of the test sample.
METHOD:
Method: Not given.
TGyLpPe:: NAocute static
Year study performed: 1985
Species: Selenastrum capricomutum
Source: Element
Not given. basis: Algal
cell
counts
(cells/mL).
Exposure
Statistical
pMeertihodo:ds9:6
IhCosu;rvsalues
calculated
using
probit
analysis
or
other
regression models. Analytical monitoring:
Not given.
Test Conditions:
Algal Nutrient Medium: Stock andtest solution
Not given. preparation:
Not given.
Exposure vessels: Not given.
ANguimtabteirono:f rNeoptlgiicvaetne.s: 2
INnuitmiablecreollflcooandicnegn:tra2t.0i5onxs:10ficveelpllsu/smLa blank control
Lighting: Not given.
---
Water Chemistry: Not given.
Reswvys
Nominal concentrations: Blank control, 100, 180, 320, 560, and 1000 mg/L.
002989
Algal Growth Response
`Mean Cell-Count
Percent Inhibition
Cfomgool--[dzt (sceellso/7 moL)0 | [ eo03 --00|| se is o ttiee0moo0 |3 Io||
stoeooo _2tbomoooon00 || 4858 ||
Element values: 96-hour ICs=o 576 mg/L.
Element values are based on nominal concentrations.
Reversibilityof Growth Inhibition: Not given.
Remarks: Testing was conducted on a mixture as described in the Test `Substance Remarks field. The values reportedapply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS
---
The test substance exhibits a 96-hour ICso of 576 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Klimisch ranking 3. Study lacks description and recordsofthe method followed. There is no characterizationofthe test substance purity, and the study lacks confirmationofthe amountoffluorochemical proportion in
the solution.
REFERENCES
t eeeeen.
This study was conducted bythe 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C1111-2, 1985.
OTHER
EE
Last changed: 6/26/00.
002930
ENVIRONMENTAL LABORATORY
WLU Ee
:
FRESHWAATLEGRALALGCAEELLGRCOOWNTTH, INnoH.I/BmIiTION TEST
HEMOCYTOMETER METHOD*
restomd=ZT teste KART meZ/a3/65
MATERIAL TESTED:. x [0 AL 12
------.
ALGAL SPECIES* GreenAlgae.Selenastruncapricornutun
in] LE
COUNTED
no./nl
MEAN + SD
I
180
pie
118
TCual
--1
11
[2-E1 E
1110
-2
lord
2A
10
B20wall
1-3
us
2-3
1
Sowa
4
aw
.
z=in
100
1000wiafX
-s
22
z-S
20
{ E009000 100,000
1,160000
1,160,000 1,100,c00]|
| 150,000
1,646.00)
1,100,000] 1,620,000 ]
1180000 | 0500
1, 10,000]
Au0,000 1,000,000
2000|
200,000
.
986,000
s
$000
* 0.1 mn deep Hemocytometer; area of field 1 x 1 and 0.1 mm deep.
002991
TABLEEN1.VIRONSUMMMEARNYTALOFLABAOCRUATTEORTYOXICITY
oF _fin
TO PRESHRATER ALGAE
(sELenasTRUM chprIcornuzum)
Cun=-z
(ng/1) Chemical Concentration
Percent Inhibition in Standing crop (2)
Based on 48-Hr.
Cell-Count
Based on 96-Hr. Based on
Cell-Count
Cell Dry Wt.
Control
-
loo
3
go
a.
220
35
SleD
Hs
1000
88
Ics, (95% Confidence Limits) ng/13) 5,
Acceptability of Test
The Selenastrum capricornutum control cultures have met the following quality criteria:
- Exhibited log phase growth within M&TM hours (max. 48 hr.) - Acetltlasi/nmeld)a 96-hr. standing crop of |Jqx(Q cells/ml (min. 10%
*Log aceqrowtn at HB-hs. H4S,000 cells/u Ixital loading 20,500 cells full
(1) pata were averages of two replications for each test concentration.
(2) Based on cell-count (no./ml) and/or cell-dry weight (mg/l).
(3) r1ce,dPesvsailounesmwoederles coaflcuelxapteerdimeunstianlg dPartoab.it Analysis or other
.
002992
