Document JrVqDkGa0DbB9grKzKZLVmO06

AR2ac 3319 : M226 _ 3317 DuPont14965 --_ TRADE SECRET Study Title Hydrolytic Stability ofH-25435 as 2 Functionof pH TEST GUIDELINES: OECD Guideline 111 AUTHOR: Bogdan Szostek, Ph.D. STUDY COMPLETED ON: October 15, 2004 PERFORMING LABORATORY: EL du Pont de Nemours and Company HElakstkoenlRloTMaLda,bPo.r0a.toBroyxfo5r0Health and Environmental Sciences ~ Newark, Delaware 19714-0050 LABORATORY PROJECT ID: DuPont-14965 Sponsor: EW.iLlmdiungPtoonnt,dDeeNleamwaoruers1a9n8d98Company USA. `WORK REQUEST NUMBER: 15028 SERVICE CODE NUMBER: 392 - TCT ompanySaiz.PeDnrocassNot Contin TSCA COL. -- Hydrol Stability of 25435 as Function ofpi --~ DuPont 14965 GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT This study was conducted in compliance with U.S. EPA TSCA (40 CFR part 792) Good LLaabboorraattoorryy PPrraaccttiiccee S(atsanrdeavridsse,d w1h9i97c)h,aEreNVco/mMpCa/tiCbHlEeMw(i9t8h)t1he7,OEOCECDDP,riPnacripsl,es1o99f8G,oaondd MAFF Japan Good Laboratory Practice Standards (11 NohSan Number 6283), except for the item documented below. The item listed does not impact the validity ofthe study. `The test substance was characterized by the sponsor prior to the initiationofthis study. AchlatrhaocutegrhiztahteicohnawraacstedroinzaetbioynawnaIsSnOot90p0er0fcoerrmtiefdieudndlaebrorGaotoordy,LaabnodrtahteorayccPurraactciyocefStthaenddaartdas,isthe considered sufficient for the purposesof tis study. `Applican/t Sponsor: E.L. du Pont de Nemours and Company Wilmingion, Delaware 19898 USA. StdyDirector: TT i SSuC Bogdan Szostek, PAD. Senior Research Chemist 1C$e-0cTocr - 2o0y Date Applicant/ Sponsor: DuPont Representative Date ------Comp-- anySaized-- .DocsNoe t Co-- nte in TSe CA C-- Be. ee ---- AR ~~ Holy Sabi of 25455 a Fantini QUALITY ASSURANCE STATEMENT Work Request Number: 15028 Study Code Number: 392 Duron 14565 mem "The conductofthis study has been subjected to periodic Quality Assurance inspections. The Phase Audited Conduct: Report/Records: Audit Dates 06 May 2004 09-12 Aug 2004 Dates Reported to Study Director and Management 07 May 2004 24 Aug 2004 Reported by:MoMQld . Johnston Quality Assurance Auditor 50dont Date ~ _-- Compt Treolgs ~ esto2503 Frioti nn CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluationofdata obtained from this study. -- -- E >" m T I5-0crt 200 f_ a ase Dican___bST `BogdanSzostek,Ph.D. IS-eerode Date --_ ~ --_-- rr Gat Hydrolytc StabilityofH-25435 as a Functionofpf -- DuPont14965 `TABLE OF CONTENTS Page (GOOD LABORATORY PRACTICE COMPLIANCE STATEMENTcnn QUALITY ASSURANCE STATEMENT wcrc CERTIFICATION sss LIST OF TABLES ...cosnmmssmsmssmmsmmssmmmosssmmssmonnt BAST OE FIGURES ..ommmmmnsimmmmmmmpmmssrsmmssminsl STUDY INFORMATION comms SUMMARY cosmos INTRODUCTIONsussrimmssmmmmmmsssmmsssmmonsmmmmmmsnsorid STUDY DESIGN sums MATERIALS AND METHODS ...conrnsmmmmssmsmsmssmsmmmsnnans]0 A. Materials 1. TestSubstance c-- cc------------ renmol)10 --~ 23. RPesriduealp8.2aofTrBAFaTitEnSHiO3ISUo4OR3nS5. ov so ores pmm-- tl 4. Equipment... TL rerea-------- ---- 2 21.. pPHremliemianastresyut ArdeCImPUeTanIEtcrvav------ wld12 43. AAnnaaloolffyt8eyss2tTissuBbsAsitansoce.rn nm p--rE----ns--n-- it1s RA rn 20 RESULTS AND DISCUSSION wo. 21 CONCLUSIONS ..cnmmmmmmmmmmmmmmmmssmmmmmsmmsmmmsss RECORDS AND SAMPLE STORAGEwormed TABLES csrrmmmmmnmsmsmismsssmmsmm--------0t FIGURES... 35 --_ ee mime ---- Company Stniaed.DAocasNsot Contin TSCA CB. Hydrol SalofHt-25y435 a Function ofpt --~ Dupon-14965 LI OFTSABLTES Page 1. A(0N3A-LJUYNTEI-2C0A0L4)RAENSDUL$T(S08F-OJURNEA-Q20U0E4O).US STABILITY OF H-25435,pi 12, 37C, DAY0S 2s 2. A(NA1LY3TI-CAML RAAENSDYU$L-(TIS82F-MOA0RY-A02Q0U048E)O.)US STABILITY OF H-25435, pi 40, 0Cs,sDAYS0 26 3. A(NA0LY6TI-CAML RAAENSDYU3L-T(S112FMOA0RY-A02Q0U048E)O)USc STABILr ITY OF e H.25435,s pi 7. s 50C, DAYSO 21 4. A(NAILY3TI-CAMLARAENYSDU-L(T12S8:F0MOARY0-A24Q0U0)4E.O.U..S.S.TABILITYgOFrH---25--4p3--H590,, 50C, DAYS0 x 5. (MOE8ATSUUNREE20D08C)ONCENTRATIONS OF$2 TBA FOR pif 12,. 37, D:0A03-Y JUNaE2S 0m04a) AmNDm ---- 6. MEASURED CONCENTRATIONS OF 8:2 TBA FORpH 40, 50C, DAYS 0(13-MAY-2004) AND 7. MAEIASDUREODDCONCmENmTRmATrIOmNSmOFm8m:2 TmBAmFmORmplmi 7.--0, --50-- D ----A 0--(06:Y MAY-2S 004) AND 31 8 (M1E8A:MSAUYR-2E0D04C)ONCENTRATIONS OF 8:2 TBA FOR pi 9.0, 50C, DAYS 0(13-MAY-2004) ANNDT . ~~ 9. DAILY TEMPREAE DINGRSOFATHETSHAU KINGRWATEER BATH... 35 10. pH MEASUREMENFTOSR DAY 0 AND DA5Y. amm---- -- _-- CompanySized.DAocszNot ConTStCAiCnB. Hydrolytic Stability of25435 as a FunctionofpH ~~ DuPont 14965 LIST OF FIGURES Page 1. EXAMPLE CALIBRATION CURVES OBTAINED FOR MONITORED [ j-- 2. BEXLAAMNPKLSEACMPHLREOMFOARTOpGHR7.A0MBSUFOFFEMROONNITDOARYE0D..[. JOBTAINEsDwtFtOeRnAtammm----tl 3. CEAXLAIMBPRLAETICOHNRSOTMAANTDOAGRRDACMOSNOTFAIMNOINNIGT$O.3REgD/L[ H-2S435.O.BT.A.INcErDsFOnRnAe: 38 4. DEXAAYM0P, LpHE 7C.H0 RHYODMRAOTLOYGTRIACMSSTAOBFILMIOTNYISTAOMRPELDE.[ ccc OBTAINED FORrrr 5. DEXAAYM$P, LpHE7C.H0 RHYODMRAOTLOYGTRIACMSSTAOBFILMIOTNYISTAOMRPELDE.[ .. OBTAeInNsEDrFmOmRmmmm----h 6. REPRESENTATIVE CALIBRATION CURVE FOR 8-2 TBA. rer ss a 7. ARENPDR$E1S5E@N/TLAT8I:VE2CTHBRAOIMNATTERONGARLASMTSANODFA1R0D4.pg 8.2 TBAo CALIr BRATs ION STANDARD nit 8. REPRESENTATIVE CHROMATOGRAMS FOR DAY 5, pH 7.0 BUFFER BLANK SAMPLE... 43 9. SREAPMRPELSEEANTNADTSI1V5EpCg/HLRDO.3M-A2 TTOBAGRIANMTESRFNOARLDSATYAN$,DpAHR7.D0.H.Y.DROr LYTIrC STr ABILITY -- ~ 10. RSEPAREMSEPANNTDLATSEI1V5EpgC/HL RD-O3M-2ATTBOAGIRNATMESRFNOARLDSTAAYN0D,ApRHD.7..0.HYDROLYsTsICsSTABILITY. errr --~ _-- `CompanySeized. Docs Not Contain TSCA CBI. -- AR Hydrol Sability ofH-25435a a Function ofpif Dupont14965 STUDY INFORMATION `SubstanceTested: [ ] Synonyms/Codes: ++ H2s43s 1 0 1 Haskell Number: 25435 Composition: [ 1 [ 1 [ 1 [ 1 [ ] [ 1 Known Impurities: None identified PN Physical Characteristics: Amber brown liquid `Stability: The test substance appeared to be stable under the conditionsofthe study; no evidenceofinstability was observed. `StudyInitated/Completed: May 4, 20/0(s4ee reportcoverpage) In-Life Initiated/Completed: May 6, 2004 July 6, 2004 --_ CompanySaaizt.tDoeesNot CeostcTSsoCrAiCtBne I. t A226 -- Hydrolytc Sabillyof25435 35a Function ofpil. DuPont14965 SUMMARY a`tTh5e0hyCdraonldytpicHst1a.2b.i2l1it3y7ofCH-fo2r5f4i3v5e dwaayss.te|sted in buffered aqueous solutions at prHepr4e.s0e,n7t.i0n,g,9.0 M`meaajsourrcehdemciocnaclenstpreactiieosnpsraensdenrteicnovHe-r2i5es4o3f5inwdeirveidmuoanlit[ored during the hydrolyticJstraebpiolritteydstausdy. eavvaelruaagteectohnecheyndtrroaltyitoincs satnadbirleitcyovoefriHe-s25f4o3r5D.ayTh0eanadveDraagye$perrecpelinctatreecsoavmeprlieess,fworerteheuDsaedyt0oand pDearyce5n,tprHeco1v.e2rrieepslifcoartethseaDmpalyes0 waenrdeD7a6y:5,5.p9H%4a.n0db6uf3fe+r2r8ep%l,icraetsepescatmipvleleys.wTerhee 9a7ve+ra3g.e0% and r9e5pl+ic4a.t4e%s,amrepslpeesctwiveerley.92T:he1.a1v%eraangde 9p0erce0n.t9%re,croevsepreicetsivfeolry.theTDhaeyav0earnagdeDpaeyrc5e,ntpHrec7o.0v.erics. for the Day 0 and Day 5, pH 9.0 replicate samples were 96 0.6% and 95 = 9.39%, respectively. The data H-25435 obtained fwoarshdydermoolnysttircastteadbitloitbyeohfyHd-ro2l3y4t3ic5alaltypsHtab1.l2e at pH show 9.0, pH 7.0, and pH 4.0. the differenceofaverage The r1e0copevrecreinets. foHromwoenvietro,readp[recipitate was obJsbeertvwedeeanftDerayad0diatniodnoDfatyh$estaesmtpsluebssitsanlacregetor tthhean aPcHeto1n.i2trbiulfefearn.dTehxetepnrdeecidpistoantiecawtaiosn,dieffsipceuclitatlolycofomrpltheeteDlayydi5sssoalmvpeleesv.enThafitserloadwdeirteidonthoef ~ roebcsoevrevreidesrebceotvwereieens,theespDeaciyal0lyanfdorDtahye b5issaemstpelrse.s iTshmeoresftorlei,ketlhyebeocbasuesrevoedfocbhsaenrgeveidn stohleubaivleirtayge problems rather than hydrolysis. 1`T0h8e-2meTaBsuArfeodrcaonnyceonfttrhateioinnvoefst8i-g2atTeBd ApHsdi.d Tnohteionbdsiecartveedancyonhcyednrtorlayttiiocndsoegfrad-a2tTioBnAocfaHn-2b5e435 seixgpnliafiinceadntbiynctrheearseeosfid8u-al28T-2BTABcAonpcreensternattiionnthoebsteersvtesdubbsettawneceen(HD-a2y54035a)n.d DTaheyre5 swaamsplnoe.s for anyofthe investigated pHs. -- --`Company Saiz. Docs NotContinTSCA CBI. ARs Hydrol Sabilty ofH-25435 as Function ofpif DuPont14965 INTRODUCTION 7T.h0e, apnudrp9o.s0eoafqutehoeusstbuudfyfewrassoltuotiionnvse.stiTghaetestthuedyhywdarsolcyotnidcuscttaebdiliatcycoorfdHi-n2g5t4o3t5heipnrpotHoco1l2:, 4.0, "Hydrolytic stability ofH-25435 as a functionof pH", which was pattemed after the OECD mGuoindietloirneed 1t1o1i%n"vesAtdidgiattieoniafl8l-y2, tThBeAconiscernetlreaasteidonforfotmhethe8-t2esTtesluobmsetarnBceAdlucroihnoglt(h8e-t2esTt.BA) was STUDY DESIGN 9A.0praenldimaitn3a7ry t0es.t1waCsfopreprfHor1m.2e.d IoFnletshsetthesatns1u0bs%toafncteheatt5es0t%su0b.s1taCncaethcyadcrhoolfytsihseips Hobs4.e0r,ve7.d0, pafetrefror5mdeady.s,Itnheadtdeisttisounbtsotatnhceetewsatssucbosntsaindceereddetheyrdmrionlayttiicoanlldyursitnagblteheanpdrenloimaidndairtyiotneaslt,ttehseting was samples were analyzed for 8-2 TBA. MATERIALS AND METHODS A. Materials 1 Nome: Test Substance H2s43s `Composition: (t 11 {{ 1 1 t ) Haskell number: t 1 25035 2. Residual 8-2 TBA in H25435 sReasmieduaanlal8y-t2icTalBmAetchoontdenats dinestchreitbeesdt isnubAsntaalnycseis(Ho-f285-423T5)BwAaisndMeettehromdisn.edFibvyeLreCp/liMcSatuesisnagmptlhees o(fteHt-ra2h5y4d3r5ofu(raapnper)oxwiamsataedldyed4,5vmigal)s wcerirmepeidn,traondducseodnitocagtleadssfvoira1ls.,5 hwoeuirgsh.edA, 10.01mmLLoaflTiqHuFot of (diDs-s8o-l2veTdBsAa)mpwlaeswaaddsetdr.anCsfaelrirberdattiooan sgltaasnsdaLrCdsvwiealreanmda0d.e9 imnL10ac/e9t0on%itTrHilFe/aacncdtoinnittreimlael isntathned.ard ~ range from 100 0 10200 ng/mL 8-2 TBA. e -- e Company Sunitized. eDocsrNo-- tConsi-- nTSC-- A CBI. ------ R26 HyHdyoidsriooSaSbubiilliiyyoorf HO-2s5i43s5 maakuucntcioonnooffpgth DDuupowois1s49e6s5 Lh 3. Preparationofbuffer solutions Tsuhiedefloilnleow1i1n1g.b0uffer solutions were prepared as described in the Annex to OECD a pHLZ "oSTfihhgeymd0ar.)o2ciMhnl2op5ro0itcamsaLscioiudfm(wc3ah6tl.eo5rr-.i3d8Te%h(,eKSC0iI.g)2mwMaa)shiypndr2reo5pc0ahrlmeoLdribwcyatadeciris.dso(TlHvhCieIn)gpwH3a.7s13.p2grbeoupffafKreerCdsbo(lypuudtriiioltnuytwi9an9gs.40.%5,mL pfslroaedspikauramenddhybbdyrriopnxlgiaidcneig.ntgh1e2f5lamskL0ovf0o.l2uMmeKwCitlhawnadter1.61.T3hmeLpHooffat0h2e bMufHfeCrIwsaosluatdijounstinetdo0 51.020w-imtLh bo pH "pTohtea0s.s1iuMm hsyoldurtoigoenonfplpiotthaalsasteiu(mpuhryitdyro9g9+e2n%p,htShiaglmaat)e wins25p0rempLaroedfwbaytedri.ssoTlhveinpgi5.41.gobfuffer s0.o1luMtiopnowtaassspiruemphayreddrobgyenplpahtchianlga2tme sLoloutifon i0.1ntMo asoSd0i0u-mmhLydflraosxkiadnedsoblruitnigoinngantdhe2f5l0asmkLtoofa volume with water. The pH ofthe buffer was adjusted t0 4.0 with hydrochloric acid. pH ~ "pThhoesp0h.a1tMe, smoolnuotiboansoifc p(oKtHas;sPiOu,m ppuhroistpyh9a9t.e5w%a,sSpirgempaa)reidn b25y0dimsLsolovfinwgat3e..4gTohfpeoptHas7sbiuufmfer sthoelu0t.i1onMwapsotparsespiaurmedphboyspphlaatceisnoglu1t4i8omnLntoofa 05.010M-msLofdliauskmahnyddrborxiindgeinsgolthutifolnaaskndto2v5o0lmumLeof with wate. The pH ofthe buffer was adjusted to 7.0 with hydrochloric acid. a pu: "dTihseso0l.v1inMg 1bo.r9i0c8aocifdK(CH!5(BpuOr)itsyo9l9ut.i0o%n,iSni0g.1maM) apnodta1s.s3i5um ochflHorBiOdes((KpCur)itwya9s9p.r5e%p,arSeidgmbay) in 2so5l0utmioLnoifn w0a.teMr. KTChleapnHd 91.006.b5ufmfeLrosfolau0t.io1nMwassopdrieupmarheyddrboyxpildaecsionlgu2ti5o0nminLfoo2fa5000.1-mML HflBas0k; ahynddrobcrihnlgoirnigc tahceidflask 0 volume with water. The pH of the buffer was adjusted (0 9.0 with Wpuaritfeicuatsidonfsoyrtbeufmf.erTphreepawraatteironrewsiasstiovbiltayiwnaesd f>ro1m3.a2 BmaemgsatoehamdecNnA.NOApllurbueffDeirasmolountdionwsatweerre scetleliullzoesde parcieotrat0e ufisleterb,yTfhiletebruifngfetrhsrooluugthioCnosmwienrge SstoerrieldizaattrioonoFmiltteemrpseyrsatteumrsewith 0.22-pm --~ r -_-- Compe any Sais. DAostRNoonan TSCA Co B. Hydrol abilityof254351 Functionofpt Dupont.14965 m 4. Equipment patiteoMrectBe:arh APOrrNeicSoinsCiMOoondMReeolcdi2pd5r0o3c0Aa2l3Shaking Water Bat, Model 50, rcision Btaolmanacteic Pipers: Conrifuge: SMReoetrseaerlacLAheEgP-eo1n0d100R0T, 1000,S00; Eppendort GVlaaressVei:als: (M1Fui0llmSiitlbucobroonsvei)oli,rc0eat.re1,SdeVirWcukmR(TSyuppeelcRo)e.sTihne viiless a(nSudpselpcto)wweirteha20tomlmavBdriac12S1tCafor 50 min before se. B. Methods Preliminary test 3`T7heCpfroerlipmHina1r.2y.teTsht ewassampeersfaomrpmleedpornepHa-r2at5i4o3n5pratocSe0duCreataenadcshtoufdtyhdeespiHgn4.w0a,s7.f0o,l9l.o0weadndfoart eachof the pHs tested. Approximate4l5y mgofthe H-25435 was weighed into sixteen 10-mL caautpopceldavweidthgltahsesavliualmsiannudm3fomilLcoovfaeprpirnogptrhieaptaertbouffftehresosleupttiaonfawcainsgatdhdeedin.siTdheeofvitahlesvwiaelr.e Ecirgihmtp ofthe vials were processed for analysis as the Day 0 samples. The remaining cight samples were -- `wwraatperpebadtwhiftohr tfhieveadlauymsinatumthefoailp,psroupbrmieartgeetdemipnetrhaetuwraet.erEibgathht,vaianlds schoanktaeinnaitng1o0n0lryp3mminLtohef appropriate buffer were prepared to serve as the blanks. Four of them were used as the Day 0 blanks and the other four were wrapped in aluminum foil and placed in the water bath to be the Day 5 blanks. 8-2 TBA stock Additionally, solution (4.5 six uL vials of 10 containing 3 40 mg/L. 8-2 mL ofappropriat TBA). The vials e buffer were were crimp c spiked with apped with the aluminum foil covering `used astheQCsamples the for 8pa-r2toTfBtAheansaelpytsaisfaocninDgatyhe0.insTihdeeooftthehre vial. three Three ofthe vials were vials were wrapped in `aluminum foil and placed in the water bath together with eightofthe test substance vials. These served Day S a role of vials and QC two samples for blank vials 8-2 TBA analysis on Day 5. Fourofthe Day 0 were processed for the H-25435 determination. or fourofthe The other four of the Day 0 or Day 5 vials, two blanks vials, and 8-2 TBA QC vials were processed for the 8-2 TBA determination. 2. pH measurement and temperature `The pH was measured on Da 0ay nd Day 5 in the vials containing the buffer and the test substance, designated for the H-25435 analysis. The vials were uncapped, the pH electrode was introduced to the vials and the pH reading was taken. These vials were further processed for H-25435 analysis. an automatic tempe The temperature ofthe rature control built in. water bath was The readingsof monitored daily. the temperature The water bath has control were checked and recorded daily. 3. Analysis oftest substance ~ H-25435 is amixture of[ present in H-25435 [ - Conpiny Sant. DaohcseNot Coin TSCA GB. 1 The [ 1 10 SdHoydhroilyeicSStuabbilliityyoof Hi-254s35aassaafFuunnccttioinoonfopfipl [ DDuupPoonmtc1l4d96g5s 1 --~ (Company Ssniized. Doss Neroiet Contain TSCA CBI.mm ---- pre ZdHoydlroylyitiecSSutbalbillliytyooffHt-22s54433S5aassaabuFunnccttiioonnooff ppli ( DDuuPPoonnctl1d4s9e6s5 --_ -_-- 1 Te (CompanySanized.DoAcsrNaot Contin TSCA CB. Hydrolyic Stabilityof 25435 as a FunctionofpH --~ { DuPont 14965 ~~ -_-- 1 `Company Sunized. DAoesrNot Contain TSCA CB, --~ HyHdyrdorloyltyiiccSSutabbiillityyoofftH-h22594i3350oassaaPuFunnccttiioonnooff ppifi [ DDuuPoonti144s9665s -- ] -- --- CompanySunized.DochsNeot Contin TSCA CB. Ra Hydrol abilityof254351 Function ofp Dupont 14965 ~ `cThheemtiecsatlsuspbesctiaenscethHat-2w5e4r3e5mohnasitnoortebdedeunricnhagrtahcetesrtiudzye.d tTohdeetceornmciennetrtahteioancstouafl icnodnitveindtusaolfthe ccohnecmeinctarlatsipoencoifetshtheatHw-e25r4e3m5o.nitored during the study were represented as the total `VwiearlesrdeecsaipgpneadteadnfdorvoHr-t2e5x4ed35foarna3l0ysmiisnuwteesr.e uTnhcaepopbetda,in6edmLextorfaacctetwoansitdriilluetewdas1:a1d0d0e0d,wivtihal2s:1 aecxecteopntittrhielep:Hwamteerabseufroermeeanntalysis. The blank samples were subjected to te same procedure, `iTshoeprsotpoacnkoslo:l8umtiLonwoafteHr.-25C4al3i5brwaatsiomnasdteanbdayrddissfsoorlvtihnegH0-.20514g35ofanHa-l2y5si4s35weirne1m0amdLe boyf2 mL. aHp-p2r5o4p3r5i.ateFrdeilsuhticoanloibfrtahtieonssttoacnk.daTrdhsewcearliebrparteiponarsetdanfdoarrcdascrhainngsetdanfcreoomfan1 atloys1i0sm.gL/iLneoafror cqaulaidbrraattiiconrecgurrevsessi.onofthe peak area vs. standard concentration was usedto establish the _ --_ `Company Site. Doceseoeet CtonatintTS--CA--C--B --reeae ts ae Hydrolytc StabilityofH-25435 1s a FunctionofpH ~ Instrumentation and conditions: DuPont-14965 HMPSLTCnsIrnusmternutm:ent: ``QMuoadtetlroAgMiilcernot, 1W1a0t0e,rsAgilent CLCoPlaurma:meters: Mobile Phase: terAacMS0.C1158%, (3v01m)macexti2c.a1cmdm/,102m.Mumtithylamine in water GradiBe-nt:0.15% (v1) acetic aid/10 mM triethylamine in methanol Tim0e0(mn) %Bs0 Flow (0m2L5min) 2800 110000 002255 81101 110000 005300 115002 500 002255 CInojleuctminonTVeomlpuemrea:ure: 010c40 MToSniPzaatriaomnetmeordse:: Electrospray, negative ions CCaopniellvaorlyavgeo:le: 3220v ~~ SDoeusrocleatTieomnpeTreamtpuerrea:ture:~~ 415200CC Data Acquistion Function: `CC6B-PPmmonooesetntereooonn::SSIIRR.ooff 454433mmzz;; 00--1122mmiinn `(CCHO0P--PmIPoAnomiexseiderefsoe: SoInR:oSfI6R4o3fm4z55;m0z1;20m-i12n min CC8I-OP--PI-PIAPAmmiixxeeddcsetsrteioinon::SISRIoRfofS68585mmzz;;0-01-212mmiinn `(CC66--PP--CCBG bbiisseestcer oionn::SSIIRRooff 788899 mmyz;; 00--1122 mmiinn `(CCLB0--PP--CCB1I0CGb-isP-sCt1e0 ibiosne: tSIcRoofn:10S8I9Romfz9;809-1m2izi;n0,-12min 4 Analysis of 82 TBA `etShaemrpl(eTs dBeEsi)gntahtreodugfhort8h-e2viTalBsAeapntaulmysuissiwngeraegplraosscessysreidngbe.y iTnhjeectpiHng13.2msLammpeltehsylr-etqeuritrbeudtyl nheyudtrroaxliidzaettioonthbeefsoarmeptlheeveixatlrabcetfioorne. tNheeuMtrTalBiEzataidodnitwioans.dNoeniethbeyriDnajeyct0inngo0r.D27aym$LsoafmpIlNessowdeiruem MunTcBapEpewderbeefvoorretMexTedBEforin2j0ecotrio3n0tmoianvuotiedsaonnyalomsusletsiotfub8e-v2oTrtBeAxe.r,Tcheentvriiaflusgceodnftoarin2i0ngmiinnujtecetsedat 4ev1e5n0a1fptemr actenrtoroifmugtaetmipoenr.atIunret.hesOecccaassieso,naslalmyp,ltehsewMeTreBEbeapnldacaeqdueinouasfrleaeyezresr wato-u2l0dnCotfosreparate `ceonvterniifguhgtedstfoorrag2e0, removed minutes. fSruobmsetqhueenftrleeyz,ert,healslaomwpeldetvoicalosmweetroe ruonocamptpeemdpearnadtu0r.1e and mLofthe --_ - `Company Snized. DAo2cs2N6ot Corsi TSCA CBL. Sow Hdyrdorloylw yiteiSSaaa bbiilltyyob offHH-225u S43S5 anFuncc tionofi pit onofp__DuDr_ upoomnti.a_ 14s9e6s5 ~~ sMpTikBeEd weixtthraicnttweransaltsrtaannsdfearrrde,dctroiampgeldascsaLppCedv,iala,nd0.s9umbjLecatceedtotnoitLrCil/eMaSddaenda,ltyhsiesexftorra8c-t2wTaBsA. 82TBA. `The 1H, 1H, 2H, 2H-perfluorodecan-1-ol (97.6%, Oakwood Products, West Columbia, SC) was used as the analytical standard of 8-2 TBA. The ID, ID, 2D, 2D, 3-13C-heptadecafluoro decanol (M+5) (abbreviataesd D-8-2 TBA) was used as the internal standard. Stocksolutions (1000 mg/L)ofthe analytical standard and the internalstandardwere prepared in methanol and `were stored refrigerated. The calibration standards were prepared freshly for each calibration in 10/90% MTBE/acetonitr 10,000 pg/L 8-2 TBA. iAlec.onTshtaentcalleivberlaotfioninstteamnadlarsdtsanwdearred made in the range from 100 was used: 515 pg/L D-8-2 to TBA . `The calibration curves were constructed using the ratio ofthe peak area for ions m/z monitored for 8-2 TBA and peak area for ions monitored for D-8-2 TBA and the concentrations of PN ~ - Company Saized. DaocssNt Contain TSCA BL. HoHdrroollytieSSuaiblityyooff HH-2255443355 aasaPPuunctioiofnpnoitlp___ ~ Instrumentation and conditions: DDuuppoomnits1s49e6s5 HMPSLICnsItnrsumreanmte:ns: M20o.deWlat2e7r95s, Waters CoLlCuPamr:ameters: Mobile Phase: AteamteMrSC18,30mmx 2.1mm, 2.5 im GBr-u"mdTeietmnhe:arnioln) "a "ns o1 E0y 7TM0 625 0o0 110000 w CInojlcutmonnTVeompleurmaet:ure: ~~ 23004C1 8 0 Column Swi 2and6min MToSniPzaatriaomnemtoedres: Capillary vole: B2l5ec5u7ospray, negative ons ~~ CSoounrecevoTleampgeerature: noovc Desolation Temperature: 300C Data Acquisiion Function: D8:-2:T2BAT:BAS:IRSoIfR4of64,672,3;$02.86vmzi;n0.6 min Flow0(2mL5inin) 002255 002255 02s 5. Calculations TmohneiftoolrleodwfionrgHc-al2c5u4l3at5io(nTsabwleerse1u-s4)e.d tFoordeetxearmmpilnee,tihefr0e.c0o45vegrioefsoHf-i2n5d4i3v5idwuaaslwcehiegmhiceadlinstpoectihees eviaaclhoanfdth3emcLhoemfibcuaflfseprewcaiessawdadse:d(t0o.0t4h5e gvia*l1t0h0e0nmogm/ign)al/0c.o0n0c3enL=trati1o5n,0t0h0atmwga/sL.aTsshiegnnedomtional cw1oa0ns0ce%cnatlrcaultaitoendubseyddifvoridriencgotvheerymecaalscuurleadticoonnwcaenstarantaiovnerbaygethoeffnooumrinraelpliccoantceesn.trTahtieon%, triemceosvery `fTohleloewxsp:ecttheed4.5(noumLinoafl)1c0o4n0cemngt/rLat8i-o2noTfB8A-2sTpiBkeArienprtehseesnptsik4e.d5 siaLmpxle1s04w0asngc/alucLu=la4t6e8d0asng 8O:f28-T2BAT.BAThiinstahmeoMuTnBtwEaesxterxatcrtaicst:e4d6w8i0thng3/m3 LmoL~f1M5T6B0En.g/TmhLe. eTxhpeec%terdecnoomvienrayliscocnalcceunltaatteidon by dividing the measured concentration by the nominal concentration, times 100% "d4Te5hremivHge-ds2af5mr4po3lm5ercoeosfnitHdau-ia2nl5s84r-3e25siTdisBu:aA4l58in-m2tghTeoBfMAH.T-B2TE5h4ee3x5etxrxpaec(ct0t(.e31d,2m9Lm%)a/,1x0ai0sm)s/uu0mm.i0cn0og3nccLeon=mtpr1la9et.ti4oemnoegxf/trL8a-c2tiTonBfAora -- (19.400 gL), --_-- oa rnSzi . Docookx CominContain TSCACB --~ HHoo2le5Sub4ily3of SasaPunctonofp__Du_rom_ 1e9_ 6s RESULTS AND DISCUSSION `JTmaebalessur1e-4dpfroersHe-nt25th4e35resaunldtsafvoerramgeeascuornecdecnotnrceanttiraaotnidsonrsecaonvderrieecsovfeorriDesaoyfi0ndainvdiDduaayl5[ replicate scaalmcpulleastefdoarspaHn 1a.v2,er4a.g0,eo7f.0,co9n.c0e,nrtersapteicotnisvealny.d rTehceovaevreireasgoef[concentration and recoveries ]were mreopnliitcoatreedsafmoprlHe-s25w4e3r5e.76T:he5.a9v%earnagde 6p3erce2nt8%r,ecroevsepreicetsivfeolry.theThDeayav0eraangdeDpaeryce5,ntprHec1o.v2eries rfeosrptechteiDvealyy.0 aTnhdeDaaveyr5a,geppHer4c.e0nbtufrfeecrovreerpileiscaftoerstahmepDleasy0wearned9D7a+y35.,0p%Ha7n.d09r5ep=li4ca.t4e%,samples wDearye59,2p:H19..10%reapnldic9at0+e sa0m.p9l%e,srewsepreect9iv6e:ly0..6T%heanadve9r5ag+e9p.e3r%c,enrtesrpeeccotviveerliye.s fTorhethdeiDffaeyre0ncaenodf average and pH recoveries 9.0 buffers. beTthwiseeinndtihceatDeas yth5aat ntdheDHa-y25043sa5mipslheysdrwoalsytliecsaslltyhasnta1bl0e%atfotrhepsHesp4H.s0, pH 7.0, (112 >one for Da0y yaenard).DaSiymilsaarmlpyl,etsheatdipfHfe4r.e0n,cepHin7r.e0c,oavnedripesH[9.0 buffers were less than 10%, except1 tDhaeyCS1s0a-mpPl-esCobLfi0stehseteprHat1.p2Hb4u.ff0earnddifpfeHr b9y.0mbourffeert.haTnh1e0%a.verHaogweerveecro,veirniecsafsoeorfDpayH01.a2nbduffer adipffrieccuilptittaotecowmapsleotbesleyrdviesdsoafltveereavdednitaifotneorfadtdhietiteosntofsuabcesttoannicteritolethaendbueffxetre.ndTehdesopnriecciaptiitoant,e was ~~ eesstpeercs.ial"liynefroerftohree,Dtahye o5bssaemrpvleedsc.haTnhgise linowtehreeadvtehreagoebsreercvoveedrireecsobveertiweese,nestpheecDiaalyly0faorndthDeabyis5 samples is most likely becauseofobserved solubility problems rather than hydrolysis Fstiagbuirliety1 ssthudoyw.s Feixgaumrpelse2c-a5lisbhroatwioenxacumrpvleescfhorro[matograms obtaiJnmeodnifotrortehed mdounriitnogrtehde h|ydrolytic:1 for a Day 0ptHes7t.s0,amDpalye,0abnldanpkHs7a.m0p,lDe,ayca5litbersattsioanmpsltea.ndard containing 5.3 mg/L H-25435, pH 7.0, `STehceticoonntAe.n2t. oBfarseesidduoanl t8h-e2rTesBuAltsionfH-fi2v5e4r3e5pliwcaastemsoeafstuhreedH-u2s5i4n3g5t,hethperococnetdeunrteofdreescsriidbueadlin 8th-e2rTesBiAduailn H8--225T4B3A5 wfarsomcatlhceutleasttesdutbostbaen0ce.1d2u9r+i0n.g0t0h4e8t%i.meAofshsyudmrionlgyttihactstthaebibluiftyfeerxpeextrriamcetnsta,ll t(hseeee5xpCeacltceudlactoinocnse)n.traTtaibolneosf4-88-2prTeBseAntinthtehemeteasstusryesdtecomnwcoenutlrdatbieon1s9o.f4 m8-g2/LTB(1A9,f4o0r0Dpagy/L0).and Dmeaaysu$rreedplfiocrattehesaDmapyle0ssfaomrpplHes1a.r2,e 4s.l0i,gh7t.l0y, h9i.g0,herrestpheacntitvheelye.xpTehcteecdocnocnecnetnrtartaitoinosnooff88.-22TTBAB.A doertigeirnmaitniantgiofnroofmreressiidduuaall 88--22 TTBBAA. in`Tthhies mteastysbuebsdtuaencteo adnifdfetrheentmeetxthroadctuisoendmefotrhaondaslyussiesdoffor hoybdsreorlvyetdicfosrtaDbialyit0y samples. samples. The The Dobasyer$vceodncdeinftferraetnicoenssoifn c8o-n2ceTnBtrAatairoencoofm8p-a2raTbBleAwbietthwteheensethe Dmeathy0odanudseDda(y2155s%a)m.plAesdefocrrepaHseo7.f0,89-.20TbuBfAfercsonacreentinratthieornanbgeetwoefeenrrDoaryfo0ratnhde aDnaaylyt5imcaaly be --~ e8-x2plTaiBnAedbcoyncaednstorrapttiioonnsobfet8w-e2eTnBDAayon0 tahnedpDreacyip$itaftoer pobHse4r.0vesdamfoprlethsisisplHa.rgeTrhtehadnifcfaernebncee of et CompanySair ized.Docs Not Contine TSCA CBI e pre ByHyddrroollyytiiecSSutabbliiltityy ooffH-22954433S5 sassaabFuunnccttiionoonfopfHph _______ DuDubPoomnit1d4g9e65s ~~ reexcpolvaeirnieedsbfyortDheaayna$lystaimcpallemseitnhdoidcaetrerhoirg(hTearbtlhea6n).exHpoewcteevderb,iatsoheftobhseearnvaeldytQicCalsmameptlheo.d for this dseuteoftosaanmapllyetisc.alThmuest,hotdhepienrcfroerasmeaonfce8r-a2thTeBrAthcaontcheentersattisounbsftoarnDceayhy5drsoalmyspilse.s is most likely cFihgruormeat6osghroawmss aforrep8r-e2sTenBtaAticvaelicbarlaitbiroantisotnacnduarrvde;oDbalayin5e,dpHfor7.80-2blTaBnAk.saFmipgluer;eDsa7y-150, sphHo7w.0 testsample;and Day 0, pH 7.0 test sample, respectively. c`Taasbelse, 9thseuwmamtaerribzaetshttheemwpaetraetrubraetwhatsemmpaeirnattauirneerdeactortdhiensgpefcoirfpieHd 1l.e2v,el4.w0i,t7h.0d,evainadti9o.0n. noTtn all exceeding + 0.1C. m`Teaabsluer1e0dspuHmmoafrDiazeys0reasnudltDsoafythSerepplHicmaetaessuarmepmleenstfsorfoarlpoHfth1.e2,i4n.v0e,st7.i0g,ataenddp9H.0s. waTsheat the specified nominal level with the deviation not exceeding + 0.1. CONCLUSIONS 9`T.h0eahty5d0rolCytaincdsptaHbil1i.t2y.aotf3H7-2Cf5o4r35iwvaesdtaeysst.edTihnebuHf-f2e5r4ed35aqwuaesoudsemsoonlsuttriaontsedattopHbe:4.0, 7.0, -- hydrolytially stable at pH 9.0, hydrolytic stability of H-25435 pH 7.0, and pH at pH 1.2 show 4.0 the dwiiftfherte;n2c>eoonfaevyeerara.geThreecodvaetraioebstfaoirned for monitored | However, in caseofpH 1.2 bu1fbfeetrwaepernecDiapiyta0teanwdasDaobyseSrsvaemdplaefseri adldairtgieronthofatnh1e0tpeesrtcseunbt.stance atcoetthoneibturfifleear.ndTheextpernecdiepditsaotneicwaatsiodni,ffeiscpuelctitaollcyomfoprlettheelDyadyis5soslavmepleevse.n aTfhtiesr ladodwietrieodn tohfe aovbesreargveedrerceocvoeverriieessb,eetswpeeceinaltlhye for the Day 0 [ and Da$y sJamTphleersefiosrmeo,sttheliokbeslyerbveecdaucsheanogfoebisnetrhveed solubility problems rather than hydrolysis. 1`T0h8e-m2eTasBuArefdorcaonncyeonfttrhateioinnovefst8i-g2atTeBd pAHsdi.d Tnohteionbdsiecartveedancyonhcyednrtorlayttiiocndsoegfrad-a2tTioBnAofcaHn-2b5e435 seixgpnliafiinceandtbiynctrheearseesoifdu8a-l28T-2BTABcAonpcreensternattiionntohbesteerstvesdubbsettawneceen(HD-a25y40335a)n.d DTaheyre$ wsaamspnloes for anyofthe investigated pHs. -- A CompanySniized. DpocsoNot Consin TSCA CB. -- Hydrolytc Stability ofH25435 a a FunctionofpH DuPont. 14965 RECORDS AND SAMPLE STORAGE NSepweacrikm,enDsel(iafawpaprlei,coarbalet),IrroanwMdoautnat,aainndRtehceofridnaslMraenpoargtewmielnltb,eWrieltmaiinnegdtaotn,HaDseklealwlaLraeb.oratory, REFERENCES 1. OGrugiadneilsianteisofnofroTreEsctoinngoomficChCeomoipcearlast,iHoyndaronldyDsiesvealsoapmFuennctti(oOnEoCfDp)H.,MOaEyC1D2,Gu19i8d1e.linOeECD No. 111 -- -- Company Sntzed. Doe=s Not Contain TSCA CBI -- Ar226 -- e Hydrolytic ability ofH25435 a o Functionofpi e DuPont.14965 ~ TABLES --_ Company Snitz. Does Not CortsineTeSeCAs CB. ommes ARs ~ 2 fg [1]FE [TF To : [3FFE15le|(2 Ha E # zEL] [ee] Mo][0 [2 (|Fe] J] ef i=[ELE [El [Hs Cdo LEP TF EL PE Bd2 E - - pEN 72 g ||[_08 P~ L Eg}1zi df & [FEPE N | 3 5 [I FEA RHEE BF3/28 g 2 I ESNCEEENCRENCECCER ENR: F Bi E= RRER FF] 33 ~ 4i5f [[EAEEREETT EFEERE tel} 3% ii ~ 8bg [ELF] FLFR g i [A: g ffi FE[Ag i of = [3[[A08E) os 8 gEBIL Bs E:IS J Pl Bd:3 Told [OL fe Pp 2 |[Ag a 1 i Z| ULF P| D12 [F1ee] 1 [FI] Fe z| I8z [EH [EEE (he) [l]-[] FEE Hl fg& LAzEe R TP2 FREE F # 3 ~~ gl ig [Jl A TT] 23 [Pll FL gLRICE CNTERNEEEE NER 3 [A][Sf 8-1 [ef oo 2 [I PO BE | : E3 ll [0] PI || TF= [I PO HEHERE: 3 3 iz zdo [GIF Fl Pl 15 J z 3 (F3IR PP g 332 gz 8 i :8 -[E3|] 3|z2ls2ls2lg ||:= H5Es 5N 4 z -- 3 [FF Br [gel] gf d Z| Fz [EE [ELL CIEE # i [1] i [=] ig CdH $ [1]fee [1 :eHeHe: [9 | ECaEREER 1 J FF Fe {b3E[oE | [900li[fE | EERSEEREEE | |: le] [1 2 a: i| :{ ElJ1CEEERERE 1g H | HE| [|Jto | oh ll | i 1 fo ap | EaN.E1 [HaTTlCENC;E IEoPh I1 E | | EE[E EEE 3 J EE ~ b = _ meAntee PIRNEyPr Table 5: Measured concentrationsof 8-2 TBA for pH 1.2, 37C, Days 0 (03-June-2004) and 5 (08-June-2004). rrr rr [elo Te [eels Tw [ee Tos Tw [eloTw | rrTT [T ew T T wT TT mii| a | [rrr1TT [lo me T l 7m a|] w | [rl7moTl 7 e wm a |w l| [fell Tw TT w] rrrrT 1 ~ Rew Tw] TT Sommer| 37 | INI I [oe sem 1 foe lst mw " 1 [eT s aw s 1) [eT s am e rrr TT ewTawT CTTomiie |1] IT TT [esTeal we Tw |w | [ewel Ts m1 e w | [em0s Tw ta | w rrTTT CT Te 1=] CT Soto | 35] _ _ [lemnn ] `Table 6: Measured concentrationsof8-2 TBA for pH 4.0, 50C, Days 0 (13-May-2004) and 5 (18-May-2004). rr 7 [oo To Te] [eo Too T0--T] [oo Tes [eo Tole Tw 1 rrr T1 TT ew mw [oiT omiion [v0 T |] TrrT 1 [Tole me Tw |w | [wo [oleae] m0 Tw 7 w| [wo [olea] Tw Tw |w | (rr TT --~ TTTe Tw] CT Somme | 20] Er rrrT1T [os Tw [ols Tow] HHH |Tm 1 rT T TT new Tow [TToo | [ n | [=] [we TT we Tw] [elsTeal me Tw Tw | [eo [Slee] we Tw |w_] rrrT 1 TTTae | ww ] CT Semen|] - ET A, LT `Table 7: Measured concentrationsof 5 (11-May-2004). 8-2 TBA for pH 7.0, 50C, Days 0 (06-May-2004) and Er wT] [oe v1 7 [osTT [eosTT [eesTT TTT FE -- IE aw aw 1] mT 7 IrTT 7 [[oeToolTeeeerT Tw wT Tw w TTo w on]| [re oTeo TwTw Tw] ~ rrr TT TT ew Tw] [Seto|n 10 | TT 7 Fe sw [oe se Te [ee Iss [ooIs a) TT7 sEes T --w 1 rrTTT [s Tw e wn e | wm | [oT ee TwTw Tw | [eTl wa T s w e Ts w | T rT T ew T1 w CT Somme | 05] i Table 8: Measured concentrations of8-2 TBA for pH 9.0, 50C, Days 0 (13-May-2004) and 5 (18-May-2004). Tn rrr rr [ooTo Tew [ooTo Tw |] [ooTosTmT w1 [oo To TT mm 1 [TT T1T Et CesT rT r T [ooToT ea TowTw 1 wm | [oTee TeTw |w | [o Tw o ww o Tw | --_ rrr TT TT Rew Tw | [Sesio [3n 0] rrr" [ooIs Tw [oo Ts 1 ew | [oo[55 Tm 1 [ooIs TT | rrr rT [Tae iT CEE Tw TrTTT [ooTo Tee Te| wo Tw [oo T5T ee TwTm | ww] [oo[ST Tm T77w0 -- |w rr 711 CT eeTw | CTTT Sones [50] --~ HHyyddroelSSuabbiillttyy ooffHH-2a54s3s5aassabFuunnccttiioonnooffppitl `Table 9: Daily temperature readings of the shaking water bath. DDuuoPmoen1td194696s5 [rm Tw[oe Tree] Temperstre EE EE o o eT e T ooemman|[0 30 || eT oman | m0] seToman| 0] [o omaT n | 0] TT wean [0| rT [eo w omT an| wo | Tw wan| so| [w swaT m | so| TTo ema | son] [eeToman| wo | ~~ [s 1wmas | wo | rT T eT wma |wo| oman|so | eT wma | 0] Towa |] 0Tom|asn oo] 0 mao [wa | TT eT oman| wo] oman | wo| en[oe ToTowa | To mae| so] CT wwe| so] rere vs otcnet, - CompanySnitz.Docss sNotContin TSCA Cl. ra Hydrolytic Stability ofH-25435 as Functionof pH -- - -- `Table 10: pH measurements for Day 0 and Day . DuPont 14965 -- [ -- --o he rr NommT ipw |Tesrmrei]pn| TTTTw Te Tes TTTw FeTTTw ---- TT 1 1 Te Ta Te Ta seTae] TeTe] Tr TT TT Te | eT Tan | eT Tm | Tw| Tr 1 ~ TwTm Tw an | Tw Tew C T s e] r T r STTTe] 1 Te Tm| eT Te| (---- TT 1 TT Tw | TT Te | ss Te Ts] rr 1 T e o T nT ] m] T T T T s rTomom] ---- Tr 1 C T s T e e T Toe w | C T TT w wT Tm n]| --_ -_-- Company Sanized. DpoersNeotConan TSCA CB. --_~ ZH yHdydorlo2 ltyitiecSS Sutblali byooiffls H-i25t4s 3y5 ass a Funca tionob fpiu ___n__c_ tionof DuDuoPnp otntl1dl 49966s5 --_ FIGURES -_-- To Company Sanized. DAoRcs Not Corts TSCA CBI, --~ Hydrolytc SabiltyofH-25435 ss Function ofpt Figure 1: Example calibration curves obtained for monitored] Dupont.14965 1 --_ EaRskEin, em FfErRm e EEv ~ erCompanySand.Do--gsrNTot--Consi--nTSCeACBr I ee ne --_ HtSeof125y4351 anni Figure 2: Example chromatograms of monitored | =esample for pH 7.0 re buffer on Day 0. Duro obtainedfor a blank erg Ne ree||Ul m= re) re] ee I@ |a -- = rE re ] LL LL mE ag T -- _ k : SE 5 I I | : = ComeySt BConao Ts CL --~ Ha Hydyodlsyrioca SSubaiblftiyooffuW225r 45335 acFutnctioo n ofpn i ofpl____D_ Duuopno_ tn1td194_696s5 Figure 3: Ecaxlaimbpralteiocnhsrtoamnadtaorgdrcaomnstoafinmionngi5t.3omrge/(dL H-25435. obtained foar ~ E ; =a Ti)E Tae oe | tCompe anySeized.Does NotCaoenTtSCiAC--ane l. --reeict ae -- HydrolyticStabilityofH-25435 as aFunction ofpH DuPont-14965 Figure 4: Example chromatogramsofmonitored | hydrolytic stability sample. [Fr owe TE] [Fe one lobtained for Day0, pH 7.0 5 -- 1] Lo : E- a, a ad TEE) [Em ral a Tr amd -- Eo, = TT [FE a ube won| [7 on TT EY saved ee ties `CompanySantized.DAoRe2sN2o6tConTtSaCAiCBnI Hydrol Siability ofH-25435 1 2 FanctonofpH ~ Figure 5: Example chromatograms of monitored | PH 7.0 hydrolytic stability sample. EE Te Dupont14965 Iobtained for Day 5, a Te [lL] FE a EoA rewrok [E[1 T | j we ry Soka [Fa ome i, TR [Fr om EN -- TeCome pany Snize. Docst Not Contain TSCA Ce BI, r AR26 Hydrolytc Stability ofH-25435 a a Functionofpf Figure 6: Representative calibration curve for 8-2 TBA. ompound name: 8-2TBA lrieblraattiioonn ccuorevfef:ic1i.en1t49r7=80*.x99+9-91329.,12779= 0.999878 eusvpeotnyspee: tLyipnee:airn,tOermiagiln:StEdx(clRudeef2, W),eiAgrhetain*g(:IS1/CxonAcd.s1trSanAsr:eNao)ne 12045. DuPont 14965 Response: 20 2000 4000 6000 8000 10000 ppb --~ EE CompanySuz. Docs No Corin TSCA-- CB. aRz26 Hydrolyic StabilityofH25435 1s a Function ofpi. Dupont 14965 Figure 7: 5Re1prpesge/nLtDat-i8v-e2 cThBrAomianttoergnraalmsstaonfd1a0r4d.ug/L. 8-2 TBA calibration standard and s05w1t104515 Smooth(Vh, 1:3) 100. 821BA 167315087 FISRof2chamnel5s | 7.49001496030,4502034 * o 100200 300 400 500 min 051104015 Smooth(bn,13) 1S82TBA 2S of2 channelsES- -- st"at 783347882 33340607+,v502085 | | | o 100 | 200 300 300 500 min -- Company Sized. Docas Not Contin TSCA CB. -- AR --_ HyidrpollSSublalylooff t225544y3355aasaFPuunccttioinoonfopiftt_ DDuuopmonita1s4e96s5 Figure 8: Representative chromatograms for Day 5, pH 7.0 buffer blank sample. b0l5a1n0k41;50p7H 7S;mDooaty5h(.Mn,1x3) 100 F1SIR of 2 chan4n6el3s,,5E2S36.898000 % o 100200 300 460 500 min --_ b0l5a1n1k041;0p07HS7m. oDoatyh5(Mn,1x3) i F2:SIR of 2chann4e6l7s,5E2S81.966e0+%003 % o 100 200500 400 500 min PN ---- Company Sei-- zed. DaoeseNeoeC-- ontmi TS--CA C--B. -- --------l --et --_~ Hydrolytc Stability ofH-25435 1s a FunctoifopnH DuPont14965 Figure9: Representative chromatograms for Day 5, pH 7.0 hydrolytic stability sample and 515 ug/L D-8-2 TBA internal standard. 051104538 Smooth(Mn,1x3) rep 1; pH 7; Day 5 %o 8218A 3.49 338073.0 FA:SIR of2 channels,ES463, 523 131264006 | | * 1.00 200 300 400 5.00 min --~ 051104038 Smooth(Mn, 1x3) F2:SIR of 2 channelsES- rep 1; pH 7; Day 15-8:2T8A 467,528 2.3026+005 Too 3.46 635915 % o 1.00 200 300 400 5.00 min --_ Compe anySe atze . Doss NotContrin Te SCA CrB. e R22 -- Hydrolytic Stability of25435a Functionofpif DuPont 14965 Figure 10: aRenpdre5s1e5ngta/tLivDe-c8h-2roTmBatAoginrtaemrsnaflosrtDaandyar0,d.pH 7.0 hydrolytic stability sample r0e1p291:0p4H673:7 DSamyoo0th(Nn,13) 0c 8325T1BA _ 3538105 F1:SIR of2 channels 5463,523 1406+006 * o 1.00 200 300 400 500 min -- 012404037 Smooth(Nin,1:3) rep 1; pH7: Day0 F2:SIR of 2 channels ES467,528 ha 15-38.-429TBA 283304005 75001.1 |% 0 100 200 300 400 5.00 min ~~ Company Santzed. Doc----s----N--ot Ceontaein T--SCA--CB,-------------- Aas