Document JrNepJ4doaX2ZrnJ42zLQ8D5X

~ Katherine E . Reed, Ph .D . S ;aff Vice President 3 N t Environmental, Health and Safety Operations . ~ .^ ; rrn 900 Bush ANenuz . Building42E-26 PO Box 3333 1 St . Paul . MN 55133-3331 651 778 433 1 OO/ / / Certified Mail July 28, 2006 .;J l 4 J ~ J ~c~ 4fQ ~ ~ .3 Document Processing Center EPA East - Room 6428 Attn : Section 8(e) CON7'~ I ~~~ ~,BI contaj, ci Office of Pollution Prevention and Toxics US EPA 1200 Pennsylvania Avenue NW ~ Washington DC 20460-0001 <-^.ar RE : TSCA 8(E) SUBSTANTIAL RISK NOTICE ON : PFHS and PFOS ; Supplemental to Sulfonate-based Fluorochemicals, Docket Nos . 8EHQ-1 180-374 ; 8EHQ-0381-0394 ; 8EHQ0598-373 Dear Sir/Madam : Prelimina ry data from an on-going study sponsored by 3M th at is being conducted by TNO Laboratories in Leiden, Netherlands suggest an effect of perfluorooctanesulfonate (PFOS) and perfluorohex an esulfonate (PFHS) on body weight, food consumption, and serum cholesterol in 15% dietary fat fed E3 Leiden transgenic mice in vivo . Cholesterol was reduced at dietary concentrations of 30 and 100 ppm for PFOS and 60 and 200 ppm for PFHS . Food consumption and body weight were reduced at dieta ry concen tr ations of 100 ppm PFOS and 200 ppm PFHS . On removal of the 100 ppm PFOS dosage-group mice from exposure through diet, food consumption an d serum cholesterol showed recove ry , but body= weights remained low . On removal of the 200 ppm PFHS dosage-group mice from expos ure through diet, body weight and food consumption showed recove ry, but serum cholesterol remained low . The lowered serum cholesterol in the 200 ppm PFHA dosage-group mice i's being obse rv ed for recovering in the on-going study . 3M believes that this is th e first observation in mice of cholesterol reduction as a result of treatment with PFOS an d PFHS, an d the first observations in mice treated with PFHS of reductions in body-weight and food consumption . PFOS and PFHS has previously been shown to have the effect of reducing body-weight and serum cholesterol in rats, and PFOS has been shown to produce the latter effects in monkeys . Therefore, 3M believes this information to be corroborative . A fi nal repo rt will be forwarded to the EPA when it is received from the test facility . If there are questions, please contact Dayna Blomquist (651-736-5413) . Sincerely, Kathe ne E . Reed Staff Vice President, Environmental Heal th and Safety Operations I IIil II, 11~111111I I I ~ E y c,' ol 0 0 1o111ai 1111o1,11 [ o( ~17 ~CQ o`~.