Document JrBVB6wvm6z8aYQDqV3KmRomO

AR226-3367 IHNHBI w iit^ M iw itf v eiim n i n j S urfm " 11* ^ R .i.H U J . ABSTRACT sr~ rw mw^ y ' j ^ v j m w ^y w v . ^wt*iw^M w w i<W *WWw^ ,Wim WVM^W ^ W ^ W ^ z. a *\<, *- *-"' s ^ ax. c ' * / ^ A , i * . , r , V . -x. n * M V . ^ * r - 1 , ' P ' * . i u lB#0rfcs wd^ w io k w trowm $$b swge toxicitytte oofetPled accRnfe to re g u la to r guideline Etefesds were w ed t o a s m & c curasiaitxttt 5pjBtji5tesfenty f ChesurTaetanaq 13tosexote?fee autoaafobroms h&cardtestmgifiu&rato fen a m ) <ro4y$3o f feese europuwfo Bused ofee tosl suite, ueifeer surfaetont ' pe$us a significantueyto OrdjsoS5 bayard to fife *3 faertlifedaijtanl* arc w n & tefe to*to fesp suretyafeef surfactant*. INTRODUCTION Fcrfluaroalkylatfid snbfemctK (FFAS) tire contpotmds cmuamifig a CftF&<{ group, m mixtures or fetm ukritm sfeatcom am such, compounds A wide range o f chemical structures felt wilhia to category metudiag perfiuottoatedsurfactants that-mayuiamately mash the aquatic environment While enviromnosta} persistence and potential environmental degradation o f these compounds arc important areas o f research, fee development o f aquatic hazarddata for use in enyiromncat risk assessment is also important for-thoso compounds that m ay reach aquatic ecosystems Wc evaluated the chrome aquatic toxicity o f a tclomcr alcohol-bused, pcrftuonnatcd anionic phospaatc surfectant {lUM&Hi and a tclomcr alcohol-based, porfluonaaiednomonic ethoxylate surfactant 3f4f5 4 i $ in 90-d early life stage tests with rainbow trout, Oncorltynchm myhSss Previous studies o f tnc acute toxicity o f these test substance rodicatcd.toal.. they were o f low to moderate toxicity to fish with 96-hri,C 5a`s o f> 1000 : m g/L and6 2 - 118 mg/L, respectively. ' V ..: ' .: REFERENCES Lewis, .A n d V.T. Wcc. 1. AqushcsafetyoiiasoictilfareitimicsurfaclMtt. Envirofl.T Smrelick.1..M.ZcaninraidR_Qemcnu. 1995. EcoioxicologyandtheuscumenlofUiasieiiliMdieU.S. EPA's OfficeorPoliutiooPreventionendToxics: CarolActivitiesandFutureNteds. pp. 127.158ta: MakingEnvironmentScience. K. Frett,N. BowenandJ.R.Stauffer(Eds.). Bccprat,Portcad. 2.' s \ t<i. 'S '* . -k % , .Ui |V . 'V . g . f c . s h V i . ^ ' .I'M. >t W* . -. Tew solstfens ware supplied 4 each repfesato msi chamber b y a eottH How ttioteimg-syatem at a rate of-ttpprodmsfaiy '7,5 L per bour (approximately 2 4 ,7 &L tost j/* |7 1*T Tic 4es`9 ff) 1' 1 -wwv- r ; ^ . TIC789^89,989.1089 h./r,n" RESULTS -- - W mMtmesta.#' IPWWWHK' !WM'*t >' mm f flft lii -m ! Mu IliillMiliillill w- "1 "W 99 WMiMMmMiii - m- m m m \ fOdlTSA [ KD 1*1 (B m m m m 503\ ( 4 Phofopwiad*- complete darkness undl day 4ft> 14 hours light nad 9 bows drabness <511minutes tmnsitbna! tight between and dark htwrvaljO for &,$smaindera fdie 4 Afrer fbtitnmg, the fingoriings were fed newiyhtttehed bnne shrimp {days 45+54}or newlyfeateaed brine shrimp andtrout chow (days 55-S9). 4 Test sohsrfons-were maintmned st approximiUely 12C 4 Daily observationsweremade forassessmentofnumberofdeadoggSrfrrslandlast dayofhatching, firstdayofsvrim-up, smvival andabnomahtiesfromhalchmg to thinning,and survivaland abnormalitiesfromthinningtotestend 4 Standardlengthandblottedwetweightofsurvivingfingcrhngswere-determinedat testend 4 Ffeorofolwnci:efeoxylafesurfactant{8*24-76$)is-a mixfuro ofcfeoxylatcs denvedfromTtilomcrB Alcohols(IBA) of fee fohmsragstructure: O V li whereQ"X5,7% 1i, 1 3 , aodx0-*ZS, t The matereomponents o f the mixture are derivee. for three T BAs (6-2 TBA, ivSi 8^2 TBA, n^Tt 102TBA, tt=9). Five o f the most intense individual ethoxylates were selected for analysis for eadi oi theTBAs, For 6-2 TBA, $-2 TBA, and 10-2 T B A fee most intense five ethoxylates represented 4 Qfivueasnetilteactitoend ewtahsobxyalsaetedsofnotrhceatodtiaTlBsiAgnaSleopbatarainteedcafoliebrtahteion, . ccounrvtceDswioefreeamchadoef tfhocr.esacclehcgierodu.epfroiofxTyBlaAlccst.rhoo.txhycla.tctcsst.,.,T.he..... substancewasnot-knowns:-The total-concentrataonnf-feetest :robsfenCi'vvBs:,8ssigned::tonach group.ofethoxyIates:fri :i; were n W ytA ni I P P ! M'.C Illlg fl lllilliil >10 FiwtDayot fiaiducg LostDayof l l l g l l Hstcftmjj FjxSl-Daj of Switnup Survivaf-Day90: pnqnn;iwayyu: Length Wetwd^it : 3.U 5.0 >io >10 >10 >10 >10 7.1 NQBC >5-}s vM 13 1,3 >5.0 >5.0 >545 >5 0 >5.0 ta 18 >50 >5.0 - >5.0 kuauiMr <**unnaa>rmbs MCH.HW'S SVtH.KJM! enu.Hitfn ifSU.HJSUE| tt i g t & ft C~ IbnU4t ftHp .......................... Analytical verification of the fluorotelomer phosphate and ethoxylate surfactant concentrations was conducted on test solutions sampled on day 0, once weekly through day 90, and at lest end (day 90). Fluorotelomer phosphate surfactant (H-25435) is a mixture of fluoroalkyl phosphate esters derived from fluorotelomer alcohols (Figure 1). The fluoroalkyl phosphate esters present in the test substance can be subdivided into three distinct groups; mono-, bis-, and mixed-cstcrs. One representative ester was selected from each group and analyzed by LC/MS. The concentrations of representative esters were represented as the total concentration of the test substance. The selected fluoroalkyl phosphate esters monitored by LC/MS were as follows: Mono esters: i-Octanol, 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluoro-, dihydrogen phosphate ion Bis esters: 1-Octanol, 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluoro-, hydrogen phosphate ion Mixed esters: 1Octanol, 3,3,4,4,5,5,6,6,7,7,8,8,8-tridccafluoro-, 2-propanol, hydrogen phosphate ion * Figure 2. Test Substance, H-24768 Chromatogram (A) and example spectrum o f the 6-2 TBA cthoxylates present in the peak at 3.3 min (B). Conservative lowest NOEC values for fluorotelomer phosphate and fluorotelomer ethoxylate surfactants in 90-day rainbow trout early life-stage toxicity tests were 2.5 mg/L and 1.3 mg/L, respectively, based on % eggs hatching and last day of hatching/first day of switnup, respectively. Both fluorotelomer surfactants are of low to medium concern for acute and chronic toxicity (Smrchek et al. 1995). Both fluorotelomer surfactants also are less toxic than many other surfactants (Maki 1979, Lewis and Wee 1983, Staples et al. 1998). Based on the hazard data presented here and conservative predicted environmental concentrations (PECs) developed using fee EUSES model, these fluorotelomer surfactants represent a low risk for toxicity to fish.