Document Jr6Xp6D1q6bmdEgONoMoJXjd6

MICROBICS MICROTOXO TEST SUBSTANCE. TOXICITY TEST Identity:Mixturecontainingperfluorooctanesulfonawtheich may also be referredtoas PFOS, FC-95, or as a component ofFC-203FC. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-203FC. Currentinformationindicatesitisa mixtureof 0.85% PFOS, 17% diethyleneglycolbutylether,74.46% water,2.34% acrylicfoamer, 1.7% alkylethersulfate2,.55% sodium octylsulfate0,.12% sodium nitrit0e.,09% sodium molybdate,0.85% triethanolaminea,nd 0.04% tolyltriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: MicrobicsMicrotox@)"BASIC" Procedure GLP: No Year Completed: 1997 Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotox@ Test organism source: Microbics'Reagent. Test conditions: Diluent: MilliporMeilli-QTM water Test temperature: Not noted. Stock and testsolutionpreparation: A 2 g/L primarystocksolution was prepared bX dissolvin2g00 mg oftestsubstance in100 mL of MilliporMeilli-Qwater. The pH ofthe stocksolutionwas 6.6.An osmotic adjustmentwas made using200 mg NaCi dissolvedin10 mL of stocksolution.Appearance oftestsolutionswas noted as "cleae'. Alltestsolutionswere made by proportionadlilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss RESULTS Nominal concentrations: Blank control,125,250, 500, and 1000 mg/L Element value: 5-minute EC50 = 604 (572 - 637) mg/L 15-minuteEC50 = 444 (423 - 466) mg/L 30-minute EC50 = 363 (349 - 376) mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-203FC 30-minuteEC50 forPhotobacteriumphosphoreum was determined to be 360 mg/L witha 95% confidenceintervaolf 350 to 380 mg/L. Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133 DATA QUALITY ReliabilityK:limischranking= 2. This study meets allcriterifaorquality testing.However, sample puritywas not propedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M EnvironmentalLaboratory,St.Paul,MN, Lab Request number R1335, 1997. OTHER Last changed: 6/26/00. ENVUTONMNTAL LABORATORY: TEST SUBSTANCE: F-C-*ZC)3@'C @UCROTOX PREP SHEET LR: S@ Date: -?, t-1- q -1 Analyst. @X v-5 1. Preparadon of StockSoludon MasslYot TestSubstance..200 *Fin& Ditudon Volume.- * Dihieft- IV\" -LQk Loa)?er oo I * FinalConcentrationof Stock Solution: 2.pH Adjustment ofStock Soludon InidalpHofStockSolution: (o-(o pHA djwted to. *pHAdjumedwith- 3.Osmodc Adjustment of Stock Sointion Osmode Adjuster.- Comments: I i I e)r\ a3a 4.Appearance ofpH and OsmoticaUy Adjusted Stock Solution * Color.- OP.4L)UE (circloene) * Comments: S.Comments FILE: R1335.K5 F("-203F(" A Ml(;RO7'(,')DXATA RYPOBT Basi(: Tc--6t, 17 TK'@, @) m in iii. M R P:F? '31 9 6. :3 T 86. :i!@ 1).o (74A M @li@ 4 "1.-,1.4/ 4 125. 00(.)() 1-@7 12!.).0000 14:" !):34.4/' 6 3: 0.312 4 9' 65.69 250 .000(') O..96b 4'7 . @)OO.0000 8,9.96/ 85 0 0.753 9 7 4 . 4'@/) 3(,J.1)5 1000.0001.) 1.807 8 84.66/ :?6 looo. [,)of)() 1.880 4 for -.:ilciilaticnE 6(.)3.5mg/L (91,)%fl't)NFIDEN(@'REAN(3E:5'(1.@, "l'o E F F P C'I' AV 7 44.,'@ 4 @J 4.4 6 E). 43. 64-8 1 :-,4 0. e, 0.5 ,--"ignature (@ONTINIJEI) TO NEXT PA(3E ... TEST DATE: 3 7 7 TIME: IP3 '3O-C -7 FILE:l@1335.K5 PAGE 2 1 (-)Zf)LIT@DDDDDDDDDI)DDD.I?DDDDDDDDDDDD-Z)I)DDDDDDDDDD.DDI)DDDDP.I.)DDDD-nDDDD ,3SLOPE 1.1797 3 3 G3 A m A 3 3 3 1 - ------ el 6 50 TOOO CoNrFNTRATTON IOE4 ES'rTMA'rINC-4 FWIJAI'I(')til:@(.)G 0.8450 x 1) 78 .9.5%CC)NFTDFNI'b: FAiT(-)R: 1.OF) @99 FOR EC50 ")FFF'IC'IENT i')FT)b-"I'FRMINATOTN:' R'2 0.99676 L4 OT-7 MICROTC)X DATA B,FPORT FILE: R1335.Ki!) FC-20.*:')b'(,AFFP 7 -14'( TKS Test 't'ime,1:5 N(JMBER 9 f). f...oritrr)]Ciel. 3 6 I Ir '71).0 CONC. ().0 riit ,i,7-)- A(3.u@sj Na('l ('13/(7@AMMA (-1-.-8-2-89----- 9 % EFFE(:T ----- AV % F)IFF. i 98.44/ 6@.'75 i:":-()()Oo 0.?41 (33.87/ 65.'77 1,@''c: 1)0 0 0 0.183 :1 9:1.44/ F)4-0() 2r)0.01)00 0.434 # 4 9'7.3!/ 53.96 2t)(.)."-)000 0.495 # 15 95,16/ 35.55 500.0000 1.219 0 6 89.96/ :35. 2.3 50().0(1(()) 1.117 # 7 94.42/ 19.73 1000.0000 :).967 # 8 84.65/ 17.36 11)1)0.0000 3.042 # ("R :-Co(,'ORRECTI@N( FACTOR = 0.8289 # Used for cal(--' ial,7ktions 19.4 15 . :it)3. 33.1 54.9 @)2 .8 74.8 75.)' l'@.4 a 31.7 1.4 53.8 1.1 75.0 fj.2 ECR)O443.*7 mg/L I-I'I-)NFIDENCREANC-,E:422.'( '1'04L'6.7) Signature CON'F'@NIJED 'ro NEX'P PAGE ... TL',Z--IDTA-E1 l'IME '7-cy -7 FILE: Rl.*I:)!K)l.r, PA ('bI,'2 I ZDDI)1)1)1)[)DDI)I)I)DDDDDi.)iji)@i)DDDDDDDDI)DDI)DDDDI)DDI)I)DDDDI)-I)DDDI)I,).DDDDD.1) 1.34,9-1 A 3 m "4 A > 4 3 EC50 10 L@I)DI)DDI)DDI)DDD. DDDDDD- DDDDDDDDDDDDDDDDDDD^ 1()0 1000 CONCENTRATION D,)DDDDDDVDDDDDDDDDDY IOE4 3 3 3 lOE5 ESTIMATING EQUATION: LOG ('o= 0.7391 x LOG b 95% (,'ONFIDENCE FACTOR: 1.04968 FOR EC50 COEFFICIENT OF DETERMINAI'ION: R-2 0.99635 +2.6471 FILE: PI:335.K3U FC-203FC AFFF MICROTOX DATA REPORT B a s.1c I'tott, :i-l't 9'7 Test Time: :30 minutes NUMBER ------- Contrc.>l Control TO/IT ------------ 95.31/ 73.01 96.36/ 73.74 C()NC. ----------- 0.0 0.() 1 98.44/ 60.31 126.0000 2 93.87/ 58.48 125.0000 :3 93.44/ 45.74 250.0000 4 97.31/ 47.30 250.0000 5 95.16/ 26.99 500.0000 6 8 9. 9!-)'./ 2'1.71 50Ci.0(.)Oc't 7 94 .4'@"/ 11.33 10 1)t.)(.)0 0 1) @3 84-651/ l'-.-9'(3 1()()0(0)(.)0 't',=R CciCORRE('I'TON FAC'roR -- 0.7656 # (Jsed foi, (--aicul.Rtion,; Osmotic Adjiistment:NaCl (@'R/GAMMA % EFFECT AV l.').7660# 0.7653 # 0.250 4 0.229 # 0.564 1$ 0.575 # 1.699 # 1.486 # 4.045 # 4.001 9 20.0 18.6 36.1 36.5 63.0 59.8 80.1-1 so . 19.3 36.3 61.4 8o. I q DIFE. 0.7 0.2 1.6 ().l E(-"50362.6 ing/l@ CONFIDrN,",E P?AN(j'F:.*'149T.C1) 3'?8.5) Signature CoN7'INIIED TO NEX'F PAGE ... 'f'F.T 1)A'I'E T I ME: 0 -F FILE:RI335.K30 PA(.IE "I)L)P .7@,;LOPE 3697 A m A 3 4 so .10 (iDI)f)T)Z.)I)I)Dr)DI)Dl)l)])DI)DDD'J)1)D1I).DIl)nlD.I))DDDID-)L.)DDI)Dl,)DDDDDDDDDDDY 10 100 (,(,N(@F.NTRATION 1000 lOE4 ESTIMATING EQUAT.ION: LOG C = (').7283 x I.OG b 9b% CONFIDENCE FACTOR: 1.03851 (7(.)EFP'IC*IENTt)F DETERMINATION; FOR EC50 0.9,9762 +2.5594