Document JkKoLGDeeGyjNdgKob6bLz26

Po ri . 6 / 4 / 11 TECHNICAL REPORT SUMMARY BACK TO MAIN U.llB ' " ` 2- 5-81 TO: TECH N ICA L COMMUNICATIONS CEN TER - 201-2CN /Important - If report is printed on,both sides o f paper, send two copies to TCC.) D ivision Environmental Laboratory (EE&PC), St, Paul, Minnesota Projoct Commercial Chemicals R e p o rt T itle Daphnid Bioassay: CS-2151, Lot 3 (LR-6457S) To *E. A. Reiner Author(t) M. T. Elnabarawy N otebook R e fronco Dept. N um ber 0535 Project N um ber 9970012600 Report Number 048 ' Employee Number(s) 46981 No. of Pages Including Coversneet SECURITY ^ Open (Company Confidential) O Closed (Special Authorisation) 3M C H EM IC A L ^ REGISTRY ^ Naw Chemicals Reported Yes CX No KEYWORDS: (Select terms from 3M Thesaurus. Suggest other applicable terms.) CURRENT OBJECTIVE: To determine the 48 Hour LC50 of CS-2151, Lot 3 to Daphnia magna. EE & PC Div (Env. Lab) Toxicity (Auatic) R E P O R T A B S T R A C T : (200-250 words) This abstract information is distributed by the Technical Communications Center tc alert 3M'ers to Company R&O. It is Company confidential material. Replicate static aquatic 48-hour LCeq values obtained for daphnids (Daphnia magna) were 76(64-125) and 73(62-106) mg/lT The" combined 48-hour LC50 was 74 (66 - 93) mg/1. Data are presented as LC50 values with 95% confidence limits. I ' mroation l.tuisun ... ... i Daphnid Bioassay - MTE Paqe 2 February 5, 1981 BACK TO MAIN In these replicated 48-hour LC50 aquatic tests, the calculated values for Daphnia magna were 76 and 73 rag/1. Materials possessing an LC50 of this order of magnitude are considered to be slightly toxic based on the NIOSH criteria for aquatic toxicity interpretation (2). INTRODUCTION This report describes tests performed on CS-2151 (light yellow liquid) to evaluate its acute toxicity to Daphnia magna. The report also provides the data generated in this study. METHODS AND MATERIALS The Daphnia magna culture was obtained from a private hatchery (A). Stock daphnids were held and cared for'at recommended test temperature (21+2C). A daily photoperiod of 16 hours light and 8 hours dark, with a 30-minute transition period, was maintained throughout the holding and testing period. Daphnids were fed up to the beginning of the test with a specially prepared food suspension combination of active dry yeast and fish food (Tetra-Min) (B). First-instar young were segregated 24 hours prior to use in assays. Each 250-ml glass beaker containing 200 ml test solution comprised a test chamber. Twenty (20) first-instar young were introduced into each test chamber within 30 minutes following toxicant addition. The acute short-term static bioassays were performed on CS-2151 using carbon-filtered well water of known composition and constant quality as the diluent (Tables 1,2). The studies performed in replicate used the following toxicant logarithmic concentrations: 21.75, 28.75, 38.75, 52.5, and 70 mg/l. Mortality or immobility, temperature, dissolved oxygen level, and pH of all test solutions were measured at 24-hour intervals. Monitoring equipment used included ASTM thermometers, Orion temperature-- compensating pH meter, a Yellow Springs dissolved oxygen meter and a Taylor temperature chart recorder. LC50 values with 95% confidence limits were calculated using the probit program and 3M TRAC Computer system. . The test protocol utilized for this study was modeled after that described by ASTM 1980 (1)'. A. Mogul-Ed, Oshkosh, Wisconsin. B. A commercial fish food of known nutritional composition. Daphnid Bioassay - MTE Page 3 February 5, 1981 BACK TO MAIN RESULTS; In this replicated study, the Daphnia 48-hour LC50 values were 76 and 73 mg/1. Chemicals possessing an LC50 value in this range are considered to be slightly toxic to the aquatic environment (2 ) . t The data reported herein are based on studies performed by M. T. Elnabarawy and R. R. Robideau. Accompanying data sheets comprise original data. A copy of this report is filed in the Environmental Laboratory. The reported LC50 values were calculated on the basis of the "observed mortality rates" and of the calculated initial concentrations of CS-2151 in test solutions. No measurements of the actual concentrations-were made at the end of the 48-hr. bioassay. REFERENCES; (1) ASTM-STandard Practice E729-80. Standard Practice for Conducting Acute Toxicity Tests with Fishes, Macro invertebrates, and Amphibians. American Society for Testing and Materials, Philadelphia, PA (1980). (2) Registry of Toxic Effects of Chemical Substances, *76 Edition, Christensen, H. E., and E. J. Fairchild, Eds., p. XV and Ci, U.S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, DC (June, 1976). MTE/cel BACK TO MAIN TABLE 1 WELL WATER QUALITY SAMPLE DATE: 12/29/80 SAMPLE TAKEN: 3N Bldg. 2-3E, St. Paul SAMPLE DATE 12/29/80 (TAKEN FROM TANK 4) PARAMETER '_ CONCENTRATION ENV. LAB WATER QUALITY BOOK REF. pH ' Residual Chlorine Ammonia Nitrogen Conductivity M-Alkalinity Total Solids Total Hardness Total Fluoride Dilute COD Total Coliform Standard Piste Count 7.7 <0.02 mg/1 <0.1 mg/1 as N 340 umhos/cm at 25C 221 mg/1 as CaCO? 291 mg/1 260 mg/1 as CaC03 0.04 mg/1 <0.4 mg/1 Negative 111 per ml 8 9 8 9 8 8 8 9 9 10 10 Aluminum as Al* Arsenic as As* Boron as B* Cobalt as Co* Copper as C* Chromium as Cr* Cadmium as Cd* Iron as Fe* Nickel as Ni* Lead as Pb* Silver as Ag* Zinc as Zn* Mercury as Hg* 0.01 mg/1 <0.005 mg/1 <0.2 mg/1 <0.002 mg/1 0.018 mg/1 <0.005 mg/1 <0.002 mg/1 0.022 mg/1 0.005 mg/1 <0.005 mg/1 <0.002 mg/1 0.13 mg/1 <0.2 ug/1 14 1 14 14 14 14 14 14 14 14 14 14 14 PCB's** <0.50 ug/1 36 Total Organochlorine** <0.50 ug/1 Pesticides 36 . Total Organophosphorus** Pesticides <0.50 ug/1 36 Sample Date 3/31/80 **Sample Date 2/26/80 BACK TO MAIN TABLE 2 ENVIRONMENTAL LABORATORY BLDG. 2-3E 8T. PAUL, MINNESOTA FOURTH QUARTERLY REPORT, 1980 WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING ' 00 M i PARAMETER TOTAL HARDNESS* as mg/1 CaC03 OCT. 30 DEC. 1 AVERAGE DEC. 29 <+ S) ~ 244 248 260 251 TOTAL "M" ALKALINITY* as mg/1 CaCO} 230 214 221 222 + 8 ELECTRICAL SPECIFIC 380 330 340 350 22 CONDUCTANCE* as twhos per cm2 at 25C TOTAL SOLIDS* as mg/1 324 300 291 305 + 17 pH** . STANDARD PLATE COUNT*** per 1.0 ml 7.7 7.6 1 6 7.6 + 0 108 139 131 126 16 '' CRITERIA OF CONSTANT QUALITY * Monthly ranges should be less than 10% of their respective averages. (ASTM Standard E729-1980.) ** Monthly range of pH should be less than 0.4 unit. (ASTM Standard E729-1980.) *** Total bacterial densities should not be greater than 500 organisms per ml. (USEPA-600/8-78-017 , p. 101.)