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ARNE -Oyoo ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyljacetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, or FC-109-X. (Glycine, N-ethyl-N[(heptadecafiuorooctyl)suifonyll, potassium salt, CAS # 2991517) Remarks: The test sample is FC-128. Current information indicates it is a mixture of 86% test substance and 14% C.-C fluoroalkyl carboxylate compounds. 3M production lot number 664. METHOD: Method: Not noted. Type: Static acute GLP: No Year completed: 1974 Species: Pimephales promelas Supplier: Not noted. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Graphed Test fish age: Not noted. Length and weight: Average length = 2 inches Pretreatment: Not noted. Average weight = 1.6 g Test conditions: Dilution water: Carbon Filtered city water Dilution water chemistry: Not noted Stock and test solution preparation: Individual weights Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Type not noted. 16L volume. Number of replicates: one Number of fish per replicate: Not noted Number of concentrations: five plus a negative control Water chemistry during the study: Final Dissolved oxygen *: 4.7 mg/L (control exposure) 3.1 mglL (40 mgiL exposure)" Final pH:* 00330 76..17 ((c4o0ntmrgol/Lexepxopsousruer)e)** Final Test temperature:* 70F + Copy of study Therefore, only fiisnanlotvalleugiebslearfeorntohteedr.eadings taken at 24 hours *t*he4050mgm/gL/Ltetsetstgrgoruopupdabtya7g2ivheonurbsecaanudsaelltodtaatlamowratsalintoyt occolcluercrteedd. in RESULTS NEolmeimneanlt vcaolnucee:nt9r6a-thioounrs:LCB5k0 c=on3tr0olm, 1g0, 20, (C.I. 30, 40, and 50 mg/L not calculated) All element values based on nominal concentrations CONCLUSIONS -- `The test 30 mglL. sample 96-hour LCso for fathead minnow was determined to be SStu.bmPiautlt,erM:inne3sMotCao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331, DATA QUALITY Rienlfioarbmilaittiyo:n Koinimtihsechmertahnokdionlgog3.y. ThSiasmsptluedypulraictykswdaoscunmotenptraotpierolnyand characterized. REFERENCES "LTahbiosrsattuodryy,wSat.s cPaounld,ucMtNe,dcboymptlheet3eMd oCnomJpuanney2,9,En1v9i7r4onmental OTHER Last changed: 5/16/00 QOOBGL V9, vommmeantiine RSE, RL ) BEST COPY AVAILABLEo YaWteegsd atr 2240 > REQUEST FOR LABORATORY WORK Lab RequestNo. ==-- ~= " TO: ENVIRONMENTAL ENGINEERING LABORATORY -- BUILDING 536N Requestor tr LabPersonnel NPoromjeacet :r1Nto2..eCrLOo.mCOPNrob RequvesatD7a.te07_m _r -= d r Sample De DataNeodnd: `Hold Sample For DaysEst.Time: "Hr DAantsB eyRse:c'd. Symp. g Date Completed: ie at: | Hes.Spent: 0 " Seven : "Tota Hardness,mg 2CaCO: CTaPlAclikuamliHnairtdyne,ss,mg m81gCaso/CCaOCylO3 M" Alkalinity, mg/l as CaCO Ea fate, mg/l 85 504. HNiotrradtee,,mgm/alllasCN1Os iWo,rdm,a/maTl4l5o5s 0 0 + g Oriei Prospo hagit&er,P THootxa.lCChhrroommiiuumm,,mymgo/l :ssCGrr. T TFeortroCcyyaanriiddee,momglill8ssCsOLN ry z rWoinn.gmsgn/elsa,maFle.#20 TT Surefnacotlant,mg moas LAS vei BCOODD.: mmga (Shor;Lov DT] Total SuspendedSolids,mg/l .__ ~~ VoTolatutaiSrloeilSus,sm,pefmngdSeedSaolids, mph ~ 2 >. * TCootnadluVcoliavtyil.e mSoolivds, mg/l T +*TTootualCBoacltefrios,rNNmoo,/110000mmI; 1 "FecalColiform, No./100 mI : AromcaolnSitraeNptiovcoogcceuns,,mNgo/.l/s1s0N0 ml = RGisdledoh mNitrogen, my es NW -~ ; Fionssal 6 aul ota 9S:JG3Comment do - aFreorr in [rect (Fe-ras dreadje-i4d [Lt 17r IT r rr rr r rrr rrr rrr rr 711 Fi--lmim------------------ r r r T r T1 r rr rr r r rr rr r r rr r rrr r Tr r Tr Tr T Fr e Trr re rr r H rr t r tt er rr [ r Irr r -- T | Tr T r Tr1 r rr r r r r r a rrrT r T 11 rrr r rr rr r 1111 rrr om Fol we 1| Sowell [owIU 70mg] JL JIL gril (F] of%SA CE Am 3> PINESShudyeFe=7a,Saal 1 FTEFh eo l24i A--s radny.dLLfA oeo03TE 300carernr i1JPF3oemR radtootno:"rRrS2 Ea s a TheaS me i nhaeSonefRINi rG LABORATORYBY ESSTT COPY AVAILABLE hy I rm etorte ignan RM l allEsi AAa un e t fi Nwae.nWniriCLmoFeiamlagT2 LeoLredPttiseoy be! onaiiogden rTsate sSeearrtee ENELoh Toeap Plotted R fru ay I "Bitution HeELAa 7 ey 1iy Ent : find ? a ra : T2a aehey EfiGg SRuerSvEH ARSdyg "72 a hes AL ay 0 CArbgh 0.0 [4 Hitomerot] 0: pal ival pi pp Survival pH pp TSruersvival[71pit] ppm iSdCHkfE RR RN A rl me HA EC I - awRET " fds] toil Sx ae dag) fy A fwrTit i ae = Se Ta] ai flzord owors ei e] =- le7k 3 fa je : = JeAeemrage iTeapabti} egerDr JELgEAaGb6eRnnR n foer 3 T phR hl T Fa ie Sve e. ch nid R HAT FEES CS h culaEon R A o pid a SEPR G mS e. TR a L Si o Hgarpo rrE R efi ptT ti firaml ae He Fraadi Nangri wh Te E fFeailaa ETTREe r 2A ld LThon "emaiet GHTaaEdLee g M n SENe L Lo bila iBATE fe es aH y E a Sn {R7Gg ni a E8 Sryke{Maei n o an SE LEAS S(fhiheedigsa hpiR alSeA r R RY Ee r iPsaeb T ER gH anL aSaMI S rpT t egs d aR g] CSh P eaT S J y A Chg ae, SER ae E L M e e e aishRJn ire piol JR cEh oS at ag S HREGIRS pr Ea e Le ORi Ye R , e aR ig L daPrEh e E,e t pgeePRRsAa T Aery R