Document JNmL6zjLe5e45NONnz1n88zBv

BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredto as PFOS, FC-95, or as a component ofFC-3017. (IOctanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-3017. CurrentinformationindicatesFC-3017 isa mixtureof0.67% PFOS, 20% diethyleneglycolbutylether,55.41% water,21% ethyleneglycol, 1.33% Hydroxy foamer, 1% sodium octylsulfate0,.5% polyoxyethylene monooctylphenylether,0.04% sodium laurylsulfatea,nd 0.05% tolyltraizole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe biodegradation potentialof the tluorochemical component of the testsample. METHOD: Method: OECD Guideline. Type: Aerobic GLP: No Year completed: 1996 Contacttime: 28 days lnoculum: Polyseed NX 2 capsules,Lot C205, Polybac,Bethelem, PA RESULTS Chemical Oxygen Demand (COD): BiochemicalOxygen Demand (CBOD): 5-Day: 1O-Day: 20-Day: 28-Day: 563,800 mg/kg 261,100 mg/kg 246,100mg/kg (replicate) 422,800 mg/kg 446,100 mg/kg (replicate) 507,100 mg/kg 478,700 mg/kg (replicate) 490,900 mg/kg 508,100mg/kg (replicate) Remarks: Testingwas conductedon the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS No conclusionsgiven. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking= 2. Testingmeets allcriterifaorqualitytesting. However, sample puritywas notpropedy characterizedand the study lacks analyticaclonfirmatioonfthe amount offluorochemicalproportioninthe solution. REFERENCES This testwas conducted by ASci Corporation,DuluthEnvironmentalTesting DivisionD,uluth,MN atthe requestofthe3M Company, Lab Request number N3688,1995. OTHER Lastchanged: 6/26/00 AScl Corporation/ASci-DuluEh Envimninental TestingDivision SubmiuW to 3M Company - 4/96 AScl-DETD Study ID 15010-068 N3688 BOD REPORT FOR 3M COMPANY Submittedto 3M Company EnvironmentalTechnology and Services 935 Bush Avenue Building2-3E-09 St.Paul,Minnesota 55133-3331 Preparedby ASci Corporation/AScl-Duluth EnvironmentalTestingDivision 112 EastSecond Street Duluth,Minnesota 55805-1701 APRIL 1996 ASel Corporation/ASci-Duluth EnvirennwntalTestingDivision Submiucd to 3M Company - 4196 ASci-DEM StudyID 05010-0" nis reportlisttshedata,informabon,and calculatiounsedtodeterminatioonf theBOD and COD for the testchemical. COD = 563,800 Mg 02 / kg chemical Datesof BOD test:3/19/96-4/16/96 Water bathtemperaturerangeduringthetest:19.6-21.0*C Lot number of PolyseedNX: C205 TABLE 1. SOD DilutionInformationfor N3688 Sample ID (mg/L) Stock Solution Concentmtion (mg[L) Sample Volurm (rni) Sample ConcentrAtion (mg/L) 10 994 3.0 9.84 18 994 5.5 18.04 04 25 984 7.5 24.60 50 984 15.0 49.20 TABLE 2. BOD Blank Calculationsfor N3688 SainpleID Day Blank Blank Blank Btu& 5 10 20 1 28 FIRST REPLICATE InitiaDl.O. FinalD.O. D.O. Drop 9.43 9.36 0.07 9.43 9.20 0.23 9.43 9.34 0.09 1, 9.43 1 9.26 1 0.17 SECOND REPLICATE InitiaDl.O. Fumi D.O. D.O. Drop 9.43 9.43 9.43 9.43 9.33 9.37 9.37 9.11 0.10 0.06 0.06 0.32 AScl Corpor*Lion/AScl-DuluLh EnvirmuncntalTc4tingDivigion Submitted to 3M Company - 4/96 AScl-DETD Study ID .15010-M TABLE 3. SOD Seed CorrectionFactorCalculationsforN3688 Scod Conc. Day MUBOUIC 10 5 10 10 10 20 10 1 29 FIRST RY-PLICATE SECOND REPLICATE Initial FuW D.O. SCP D.O. D.O. Drop blitiai Final D.O. SCF D.O. D.O. Drop 9.32 9.32 5.33 3.17 3." 6.15 0.60 1.23 9.32 9.32 5.31 3.75 4.01 5.37 .90 1.11 9.32 4.11 5.21 1.04 9.32 3.09 6.:!4 1.25 9.32 1 4.15 5.17 1 1.03 9.32 1 4.16 1 5-16 1 1.03 Averagc SCF 0.80 1.17 1.15 1.03 SCF = (D.O. drop * nilseed in test) ml seed in seed blank. TABLE 4. BOD Reference'and Tox Blanks Calculationsfor N3688 Conc. Day SCF mg/L 0 49.20 5 0.80 5 0.90 1 FIRST REPLICATE In;t@-I D.O. Fumi D.O. D.O. Drop Coffected BOD Drop mg[O SECOND REPLICATE Initial Final D.O. D.O. D.O. Drop Correctod Drop Boo mg/L 9.42 4.23 5.19 4.39 mo 1 9.42 1 429 5.13 4.33 217 9.39 0.07 9.32 8.52 426- 9"3 9 0.10 9.29 8.49 425 a Referenc=e 150mg glucosaend 150mg glutamiaccidinIliteBrOD dilutiwoantert,esteadt2% dilution. BOD mg/L (correctDe.dO. drop) 300 ml BOD volume 6 ml reference. -2- TABLE S. BOD CalculationsforN3688 AScl Corpomtion/ASci-Duluth Envimanwaul TestingDivision Submiucd to 3M Company - 4/% ASel-DM StudyID #5010-068 Conc. Day SCF m&IL -9.84 4F2 6imgs/.L0 C9o-n8c4' 2148.-6004 49.20 5 --o.Bo 5 0.90 5 0.90 5 0.80 FIRST REPLICATE 1-i6.1 D.O. Fi.W D.O. D.O. Drop Coffected Dmp BOD -&/ke 9.36 6.48 2.98 2.08 211400 9.38 3.38 6.00 5.20 298:!Oo 9.40 1.62 7.73 9.42 0 6.98 283700 0*0 m can 261100 lakw D.O. 9.36 9.39 9.40 .42 SECOND REPLICATE FuW D.O. D.O. Drop Cormcted Drop 6.52 3.71 2.94 5.67 :!.04 4.87 2.18 7.22 6.42 Boo mglkg 207300 2700DO 261000 Mcan :!46100 9.94 10 18.04 10 24.60 10 49.20 1 10 n 1.17 1.17 1.17 1.17 9.36 9.39 9.40 9.42 4.03 5.33 0 0 0 4.16 -L29-0O@ *0* N!can 422800 9.36 9.38 9.4C 9.42 3.90 5,56 0 0 01 1 4.39 446100 1 Mean 44610D 9.94 18.04 24-60 49.20 20 1.15 20 1.15 20 1.15 20 , i.is 9.36 9.38 9.40 9.42 3.2.) 6.14 0 0 0 4." 507100 9.36 9.38 9.40 9.42 Mean 50710'0D000 3.50 0 0 0 5.96 4.71 479700 4MI117. 9.94 28 19.04 29 :!4.60 29 49.1.0 28 1.03 1.03 1.03 1.03 9.36 9.38 9.40 9.42 3.50 0 0 0 5.86 4.83 490900 Mean 41 9.36 9.38 9.40 9.42 3.33 0 0 0 6.03 BOD correcteddrop x 1,000,000 testconcentration. Omittedata points: Find D.O. MeaSUred at letvilower than detectionlimit. 5.00 508100 00* *** 00* MeAn 509100