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ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonamtaey; alsobe referredtoas PFOS, FC-95,or as a component ofFC-203C and L-5440. (1-Octanesulfoniaccid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecapfoltuaosrsoi-u,m saltC,AS # 2795-39-3) Remarks: The 3M productionlotnumber was 501. The testsample is FC-203C. Currentinformatioinndicateistisa mixtureof1.47% test substance,60% water,30% diethyleneglycolbutylether,3% sodium octyl sulfate3,% hydroxyfoamer,10% polyoxyethylenemonooctylphenylether, 0.12% sodium laurylsulfatea,nd 0.05% benzotdazol. The followingsummary appliesto the testsample as a mixture of the testsubstance in water solutionwith incompletelycharectedzed concentratfonof impudgies. Date may not accuratelyrelatetoxicity of the testsample with thatof the fluoruchemicalcomponent. METHOD: Method: Not given Type: Staticacute GLP: No Year completed: 1981 Species: Daphnia magna Supplier:Not given. Analyticalmonitoring: Not given. Exposure period:48-hours Test organism age: Not given. Statisticamlethods: No calculationpserformed,based on studyresults. Test conditions: Dilutionwater: Notgiven. Dilutionwater chemistry:Notgiven. Lighting:Not given. Stock and testsolutionpreparation:Notgiven. Exposure vessels: Not given. Number of replicates:Not given. Number of daphnids per replicate:Notgiven. Number of concentrations:Notgiven. Water chemistryduringthe study: Not given. RESULTS Nominal concentrations:Notgiven. Element valuesbased on nominalconcentrations. Remarks: Testingwas conducted on the mixtureofthetestsubstanceas describedinthetestsubstanceremarksfield.The valuesreportedapply to thatmixtureand notthefluorochemicaplroportionalone. CONCLUSIONS No mortalitwyas observed duringthestudy. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 4. Values arefroma summary sheetonly - raw datanotfound.Testconcentrationnsotprovided.Study lacks descriptioannd recordsofthe method followed.There isno characterizaboonfthetestsubstancepuritya,nalyticamleasurement of thetestconcentrationsa,nd confirmatioonftheamount offluorochemical proportioninthe solution. REFERENCES Test was conducted by the 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 681 IS, 1981. OTHER Lastchanged: 6/26/00. 05/30/2000 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT LAB REQUEST NO. 6811S CONTRACT LAB(S): REQUESTOR NAME: EAR EXP COMP DATE: 07/20/1981 DEPARTMENT: 0535 DATE COMPLETED: 07/06/1981 PROJECT NO: 9970012600 PROJECT LEAD: SA BEACH DATE RECEIVED: 04/29/1981 PHONE NO: 651-778-7452 DESC: FC203C L5440 LOT 501 3M FAX NO: 651-778-6176 -------------------------------------------------------------------------------- SAMPLE DATE CODE DESCRIPTION RESULT MDL or (95% C.I.) -------------------------------------------------------------------------------- 1 48-HR-EC50 96-HR-LC50 BOD-05 COD FOAM-POT IC50-ALGAE SLUDGE-TOX 2 COD FC203C CC814-28 DAPHNIA ACUTE TEST FISH ACUTE TEST - 96 BOD-05 COD FOAMING POTENTIAL ALGAE ASSAY - IC50 SLUDGE TOXICITY HOUR DUP 1 COD NO MORT ALITY HOLD SEE ATTACHED REPORT 776000 MG/KG SEE ATTACHED REPORT SEE ATTACHED REPORT SEE ATTACHED REPORT 771000 MG/KG