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TECHNICAL REPORT SUMMARY
D at*
8/4/78
TO: TECHNICAL COMMUNICATIONS
CENTER - 201-2CN
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CRL - Analytical and Properties Research
Proio,-t
Trace Fluorochemicals
Report 1-itfs
in the Environment
IRDC 137-092:
To
J.E. Long
Author(s)
Jon Belisle
FC-95/Monkey
Notebook Reference
Laboratory
Dqpt. Number
0502
PfOlOCt Number
AOoo6O6
WiVol-t VU-M be r
967
FZMPIQYQO Number(s)
88240
NCL of Pages Including
Covershoot
QEC-LIRITYI?. 0 Open
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CURRENT
OBJECTIVE:
Requestor:
J.E.
Long;
3M CHEMICAL REGISTRY
Request #A69507;
Now ChemicalsReported
E3Yes
f No
Project #9172110004
Central Research .-Xnalytical Reporl'. --Iiemical/Analysi,.i
REPORT ABSTRACT: (200-250 wyords)This abstractinformation isdistributedby the Technical Communications alert3M'ors to Convany R&D. ItisCompany confidentialmaterial.
Center tc
The level of FC-95 was determined of monkeys from the IRDC subacute
'in the serum and livers toxicity study 137-092.
CENTRAL ANALYTICAL.LABORATORY
Report No.
-----------
Date -----Zugllst-A-, -UIS -----
Subj ect: IPDC 137-092: FC-95/Monkey
Requestor: - --.,-IE. -Lc)zL-g---
Dept. Name --ZQAiCOlOZY--
Request No. -.@@t9507 -------
Dated----8./X-7-.-F-i-------
Report:
Proj. No.91.7711nn04
Reference 137-087 by IRDC was a 90 day subacute Rhesus monkey toxicity study of FC-95. Incorrect (too E-i@'-gfhe-e)ding levels were used and all animals died within the first few days. The feeding levels were lowered and the study started again as 137-092. -The serum and livers were each individually submitted for analysis. Each group contained 4 monkeys, 2 male and 2 female as follows:
Gro4p IV
Dosage Level 0
0.5 mg/kg/day 1.5 mg/'kg/,day 4.5 mg/kg/-day
Survival 4/4 4/4 4/4 0/4
SERUM ANALYSIS
Monkey Blank on Method
Dosage Level -
FC-95 in Serum (ppm)(D 2
7355M 7358,%l 7368F
0
40
0
20
0
15
7463L,,l 7466F
0.5 mg/kg/day
150
if
150
7462M 750OF
1.5 mg/kg/day
250
It
275
Our newly developed pyrolysis method was used. Precision is estimated to be 10 - 25%.
A.R. #6967
2-
August 8, 1978
The high levels of FC-95 in the control serum cannot be explained. For example, 7355M was run on 2 different days with the same experimental results. Perhaps a total fluorine should be run on this sample to determine if this level of F is present. See Table below containing liver results for further discussion (Footnote 2).
LIVER ANALYSIS
Mon.Key
Dosage Level
7355M
0
7368F
0
FC-95 in Liver(m 3000 150ow
FC-95 in Liver(12pm) 50 2041,
7463M
0.5 mg/kg/day
7000
100
7466F el
8000
100
7462M
1.5 mg/kg/day
45000
650
it 750OF
40000
600
7484M 7502F
4.5 mg/kg/day to
40000 80000
650 1000
The livers were stored in the refrigerator several months prior to analysis resulting in the separation of some liquid. The high values and pliysical size necessitates taking only partial livers in which case the same ratio (estimated visually) of solid to separated liquid was taken for analysis. In addition, recovery of FC-95 was estimated from experiments where FC-95 was added to a control liver.
Obecause of a higher than expected value for the control, a 0.133 g liver sample was analyzed for total fluorine. The fluorine value calculated as FC-95 is equivalent to 11 ppm FC-95 in the liver. As time permits, more work should be done to verify these "high" control levels.
c:R.A. Prokop
236-3
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