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M S Q G - ta. g*? Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol DuPont report # 12734 8-2 Telomer B Alcohol, C8Fi7CH2CH2OH: Ultraviolet Spectrum Test Guideline(s) Authors Mary A. Kaiser, Ph.D. (study director) Raymond E. Richardson Robert C Buck, Ph. D. Study Completion Date 16 July 2002 Report Date 21 March 2003 Test Facility DuPont Corporate Center for Analytical Sciences Experimental Station 402/5321 and E328/162B Wilmington, DE 19880 Sponsor Telomer Research Panel c/o Rand Corporation ORPEPCTEIHVOEDC 2003 APR 21* PH 2: 28 ITelomer Research Program 1 000029 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol 2 Table of Contents Title P ag e...................................................................................................................................................... 1 Table o f Contents......................................................................................................................................... 2 1.0 Sum m ary.............................................................................................................................................3 2.0 General Study Information................................................................................................................3 3.0 Materials and M ethods...................................................................................................................... 4 3.1 Test Guidelines....................................................................................................................... 4 3.2 Chemical System.................................................................................................................... 5 3.3 Test Vehicle............................................................................................................................ 6 3.4 Parameters Observed............................................................................................................6 3.5 Result Analysis....................................................................................................................... 6 3.6 Validity Criteria of the Study.............................................................................................. 6 3.7 Protocol Deviations................................................................................................................6 4.0 Results and Discussion...................................................................................................................... 7 5.0 Conclusions.........................................................................................................................................7 6.0 Retention o f Records......................................................................................................................... 7 7.0 Disposal o f Test Item......................................................................................................................... 7 8.0 References...........................................................................................................................................7 Figure Figure 1 UV-VIS spectrum....................................................................................................................... 8 Appendix Appendix A Chemical Analysis Summay............................................................................................. 8 2Telomer Research Program 00003 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol 1.0 Summary Test System: 8-2 Telomer B Alcohol, C8F 17CH2CH2OH, was used to determine its ultraviolet spectrum at 25C. Findings: There is no measurable absorption from 225 to 400 nm. Conclusions: There is no measurable UV absorption for this compound. 2.0 General Study Information Study Objectives The objective o f this study was to determine the ultraviolet absorption spectrum o f 8-2 Telomer B Alcohol, C8F i7CH2CH20H, at 25C. Test System Justification The test system was selected by the sponsor. Study Personnel Management: Study Director: Principle Investigator: Technical Personnel: Marianne Marsi, Ph.D. Mary A. Kaiser, Ph.D. Raymond E. Richardson Raymond E. Richardson Study Execution Dates Study Initiation Date: Experimental Start Date: Experimental Completion Date: Study Completion Date: 15-Jun-2001 15-Jun-2001 28-June 2002 12-July 2002 3Telomer Research Program 3 000031 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol 3.0 Materials and Methods 3.1 Test Guidelines OECD Guidline 101 ws used in this study. The ultraviolet absorption spectrum of a solution is a function of the concentration, Cj, expressed in mol/L, of all absorbing species present; the path length, d, of the spectrophotometer cell, expresed in cm; and the molar absorptivity (extinction coefficient), \ , of each species. The absorbance (optical density) A of the solution is then given by A = d 23,- ei; c, 3.2.1 Chemical System 3.2.1.1 Test Substance The test substance was obtained from Clariant Gmbh (Germany) and was shown to be 99.2% pure via gas chromatography. No other alcohol or iodide impurities were detected. The major impurity (0.8% by area) was tentatively identified as C7Fi5CF=CH20H based on the mass spectral fragmentation pattern Name: 8-2 Telomer B Alcohol Synonym: (Perfluorooctyl)ethanol Active substance(s) 8-2 Telomer B Alcohol, 99.2% CAS Name: 1-Decanol, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10heptadecafluoro- CAS Number(s): 678-39-7 Structure: 4Telomer Research Program 000032 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol H Number Lot Number: EMSE Sample Number: Concentration, nominal: Concentration, analyzed: Certificate of Analysis Date: Date Received: Solubility at 25C.: Vapor pressure: Stability: Appearance/Color: Storage Conditions: H-24691 P.00/001 E93386-63 99% 99.2% 03-Apr. 2001 15-June -2001 -140 pg L '1 0.023 mm Hg Stable at ambient room temperature White solid Room temperature; keep tightly closed 3.2.1.2 Test Vehicle The test substance was 8 2 Telomer B alcohol dissolved in spectrophotometic-grade methanol. 3.3 Test Conduct The Telomer B 8-2 alcohol was dissolved in methanol. A Varian Cary 5 ultraviolet/visible/near-infrared spectrophotometer was used to obtain the spectrum. The sample was weighed into a 10-mL class A volumetric flask and brought to volume with spectrophotometric-grade methanol. The solution was shaken and examined visually to ensure that there were no insoluble materials. Two 1-cm cells were triple rinsed with methanol then filled with methanol and placed in the sample and reference beams of the Cary 5 spectrophotometer. A baseline was collected in methanol versus methanol to ensure compensation. The sample cell was then emptied and rinsed three times with sample solution before filling with fresh sample. The sample cell was then placed in the sample beam and the spectrum recorded versus methanol. 3.4 Parameters Observed Changes in molar absorbtivity (M'1cm'1) were recorded versus wavelength (nm). 3.5 Result Analysis The output was compared to the spectrum of methanol versus methanol. Any observable absorption above baseline is noted in the report. 3.6 Validity Criteria o f the Study The instrument was calibrated for both wavelength accuracy and photometric accuracy 5Telomer Research Program 5 000033 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol 3.7 Protocol Deviations None 4.0 Results and Discussion The ultraviolet absorption spectrum showed no absorption within the wavelength region measured. 5.0 Conclusions Telomer B 8-2 alcohol does not absorb ultraviolet radiation in this range. 6.0 Retention of Records Study documents and materials will be stored in the archives of the DuPont Experimental Station including but not limited to: study protocol; any protocol and/or report amendments or addenda or deviations; all raw data; one original signed copy of the final report; laboratory-specific or site-specific raw data such as personnel files, instrument, equipment, refrigerator, and/or freezer raw data. 7.0 Disposal of Test Item After issuance of the final report, the remaining test substance will be stored at the DuPont Glasgow site until its expiration date and then destroyed by burning, unless other arrangements are made between the sponsor and the Test Facility. 8.0 Reference OECD Guideline for Testing of Chemicals 101, adopted 12 May 1981,UV-VIS Absorption Spectra (Spectrophotometric method). Telomer Research Program 6 000034 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol Figure 1. Ultraviolet Absorption Spectrum of Telomer B 8-2 alcohol at 25C. UV sprectrum for 8_2 Alcohol 7Telomer Research Program 7 000035 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol Appendix A Chemical Analysis Summary 2-Perfluorooctyl ethanol : C8F17CH2CH2OH H-24691 Analysis Summary The subject material was analyzed by gas-chromatography using a standard method to assess its composition. This analysis showed that the material comprised 99.2% of the subject material. The balance, 0.8%, was not another Telomer B alcohol, or Telomer B Iodide at a limit of quantitation of 0.02%. Further analysis by GC/MS suggested that the 0.8% was a single impurity, whose chemical structure is C7F 15CF=CHCH2OH. Finally, the subject material was evaluated by High Performance Liquid Chromatograpy - Tandem Mass Spectrometry (LC/MS/MS) for the presence of perfluorooctyl sulfonic acid and perfluorooctanoic acid at a detection limit o f 0.1 ppm. Neither material was observed to be present. Material CgF 17CH2CH2OH 2-Perfluorooctyl ethanol C7F 15CF=CHCH2OH Wt. % (+0.31 99.2 0.8 8Telomer Research Program 000036