Document JJG8yqdZrxyy1G0YYwYJgXvg2

FILE NAME Industrial Hygiene Foundation IHF DATE 1970-1971 DOC IHF069 DOCUMENT DESCRIPTION Excerpt of Study - Fibrous Dusts in Lungs of Urban and Rural Dwellers with Cover Memo atnelbia! seve La woe pmalat. oD ee : fi" fi/ fl | 412 687-2100 fi" fi/ fl ee fi" fi/ fl . INDUSTRIAL HEALTH FOUNDATION INC 5231 CENTRE AVENUE Pe PITTSBURGH PA 15232 March 24 1971 pred | Manhattan 10 Sponsors of IHF Fibrous Dust Study Mr. Dr. Mr. Dr. Hugh M. Jackson Johns Manville Lee B. Grant PPG Industries Corporation Russell Brittingham Pittsburgh Joseph L. Goodman Raybestos Corning Corporation Inc. <or will wil Attached is a copy of the appear in the Foundation's report on the IHF Fibrous Foundation's 1970 Annual Report Dusts Study which immediately Please let us know text of this if you should have report or to the inclusion of any objections to the the your company's name as sponsor sponsor Absence of a reply by April 2 will indicate approval 3 hank you Sincerely yours Encl Mrs. Sally Carlysle Office Manager 631 May 11 1970 Dr. Joseph L. Goodman .- Medical Director for Research Raybestos Inc. | North Charleston South Carolina 7 29406 Dear Joe r - a ok. IHF's research on Fibrous dusts in lungs of urban and rural dwellers has been funded by the U. S. Public Health Service and we are now only awaiting completion of Industry's portion of the funding to proceed So far HF's studies of fibrous dusts have required approximately half million dollars and projects involving similar amount It is asbestos now under way or comforting to know of your proposed will require a willingness to recommend that your company continue to support our studies at the same rate as in the nast By participating in this way we will continue to extend to your company the benefits of sponsorship including advanced notification of publications and of an opportunity to share in research planning Copies of our reprint from the Environ Enm vie ronnmtenatall Health enclosure which appeared last month will be forwarded when received Please advise how many copies you would like to receive Additional copies of the Fibrous Dust Seminar Proceedings Proceedings can also be mado prepare available to you upon request From all of the above I a critique for your own company's use If we may be of hope you can any further vv Assistance please advise gress Incidentally for your personal use am including a copy of our pro- report to the U. S. Public Health Service on Asbestos Dusts Their Fathogenic Components It should not be reproduced without getting our per- mission in advance as it is not for general consumption In addition I am sending a draft of the summaries of IHF projecta which will be included in the 1969 Annual Report If you wish extra coples of the Annual Report when it becomes available later this month please advise and they will be provided without charge of course With best regards Sincerely det sje Enclosures bec Mr. H. M. Bowman Robert T. P. deTreville M. President JHM 04931 ara a 4 Chemically asbestos dust Six gram~-portions of each type of asbestos were milled for two hours Each batch of asbestos dust was covered with aqua regia at room temperature for one hour The proportion of aqua regia to asbestos was 250 ml The dust was then transferred to Buchner's funnels and washed wpi et rh gdr isa tm illed water until free of acid A two per cent solution of EDTA in 1 Molar potassium hydroxide was prepared and was suspended in this solution for one hour and water in Buchner's funnels After drying each asbestos preparation then washed with distilled . preparations were Spex milled for 3 to 15 minutes following which standard suspensions were made of each a ; 5 Chemically- and treated asbestos dust Batches of each of the three types of asbestos were treated chemically as described under 4. After drying these batches were milled for 3 to 15 minutes the time depending on the microscopic appearance of samples examined at frequent intervals These batches were then heated in the electric furnace as described under 3 and standard suspensions made B. Intratracheal Injections All the intratracheal injections were performed under light ether anesthesia with aid of an illuminated retaining laryngeal speculum which allowed visual- shion of the vocal chords This enabled the operator to pass a long 18 gauge edle words with rounded end attached to a tuberculin syringe and inject the desired amount of dust suspension suspension between the vocal into the trachea The rats were injected with 1 ml of dust suspension C. Intrapleural Injections These injections were made with a bevelled 22 gauge needle 4 mm long and attached to a tuberculin syringe All animals were injected while under light other anesthesia in the right midaxillary line in the region of the 5th erspace Rats were injected with 50 mg asbestos suspended in 1 ml dis- filled water and hamsters with 25 mg asbestos suspended in C. ml water RESULTS General employed No significant difficulty was experienced with animals receiving intrapleural injections In contrast the intratracheal injections proved highly lethal when observed the as we had doses of 7 mg or higher were emploIy nae smud ch development of lung cancer in rats receiving in the neighborhood neighborhood of 14 to 21 ing chrysotile dust we aimed for a total dosage of 21 mg asbestos dust Because it became necessary to of the very high lethality of our dust preparations S IHM 04936 Table 1 Tabular Protocol of Rats Injected Intratracheally with Dusts Dust icroquartz a atural Chrysotile b alc tremolite c , Fiber Diameter microns 1.2 . 0.05 - 0.2 1.0 0.1 - 0.2 No. 1 Rate Done Mortality ) 62 55 50 25 mg 26 in 6 months 45 in 15 months 10.5 mg + 14 ing 90 in 18 months 100 in 24 months 25 mg 40 in 6 months 44 in 6 months | ynthetic Chrysotile d 0.02 - 0.04 55 14 mg + 45 mg 22 in 12 months 53 in 24 months eramic Aluminum Silicate e lass I rucite ilicon Carbide g 2.0 J 2-3 80 10.5 mg | 75 | 10.5 mg , 1510.5 1510.5 mg 0.5 - 3.0 22 3.5 mg morphous Magnesium | Silicate h 10 75 mg i 72 in 85 in 18 months 18 months 67 in 12 months 100 in 18 months | 47 in 12 months 73 in 24 months | 18 rats sacrificed at intervals no deaths in 6 months \ 10 in 6 months d e 1. g. h i medium nickel 31 rats and low nickel 27 rats High nickel 35 rats dust to 15 rats milled dust given to 40 rats and hammerto 25 rats each Tale with high and low natural nickel content given One batch prepared at Mellon Institute the other at Manville Research and Engineering Center Named Fiberfrax obtained from Carborundum Company kinds of fibrous glass 1 etched of 15 rats given different coated with Groups 2 uncoated , coated with starch binder , glass resin Silicon carbide whiskers from Carborundum Company Prepared by reacting sodium silicate with MgCl2 and washing precipitate Range of mortality in months of different groups JHM 04973 oem cum me Ot CC PRT WEE ORD reer dunce mere aearemewneievrreBrs