Document JJDRo1Qkp2RN4QyaX6bJ59Qe2

2-1 Ilnrkluy < of.: J net ion of Mlin-rnl Kilters in 'piumi* m flu-y niiulil Ih> liii|i|M-il in IikmiitmtriiL'il tiiliuliir Mnirtiiro of th<' Wiry""llie wu hiivv used. For u ciirWnugi'nlr efTt'W In tie cxiwtrit, the active nyurit muni remain in contiirl with the cell" or tisane for u considerable period''1 -- i.., epilhellnl pnpllltinms In miee are generally not produced except after ii||lnli'il nilmllil"! rut loll.** M inny lie thnl n "In-- I filar" farilllnte till" r<m. llnued contnct. K In nlno jsisslb]* that other fillers ami mnlerinln in com* mim use may act in a similar manner." In order to teat theae possibilities # have done a number of experiments V-.- .1, Chrysotile fibers were Immersed in a very dilute solution of befterinel sulfate' Cl :100.000). After 24 hours the'dye had disappeared from' the solution, and the fibers were brightly fluorescent. "'t 2. Chrysotile -i B.P. were suspended in a variety of liquids to test for sdsorption of B. P. on the asbestos. The liquids included (a) water; (b) online; (c) acetone; and (d). xylol After more than;six:n>onths:.theius^; pending liquids were replaced and the fibers examined for adsorbed.B. P. by ` fluorescence. In the cases of the B. P. solvents, no fluorescence different ijclrpm that of the controls'was_oherved..In.the aqueous suspensions .'it .waaljSSjBa^ ~ found that the adherence of tlie B, P.'to the fibers was such that afiuviatibnf^^?^ centrifugation, shaking, or washing on a Gooch crucible could not separate the carcinogen particles from the fibers. Figure 9 shows the mixture in white light Bnd by fluorescence. The B. P. could be dissolved off by acetone, but the fluorescence of the fibers was then no more than that of the controls. Although these results do not indicate that there is any concentration of B. P. on the surface of entrapped asbestos fibers, it appears possible that solid inspired particles such as B. P. may be held firmly for long periods in contact with respiratory epithelium. ' ' .... Arknowledffttimlt The chrysotile used in these experiments was supplied by Mr. Duym of th Asbeaton Corporation of America. The initial experimental ashing was dune for us by S. E. Gleit of Traeerlab, Richmond, California. We are grateful for the use of the R-C.A. Laboratories vacuum facilities in Prince ton for the 60 cycle ashing, and to Mr. M. S. Badollet for technical con sultation. Reference* 1. Poucaui, A. 1002. The conflict of living matter with the mineral world: The pneumoconioses. J. Clin. Pathol IS: S94-400. t Stewart, M. 1.1 A. C. Hadoow. 1929. Demonstration of the peculiar bodies of pulmonary asbestosis ("Asbestosis bodies") in material obtained by lung puncture and in the sputum. J. Pathol. Bacterial, 32:172. L Clovne, S. K- 1929. The presence of the asbestos fiber in the lesions of the asbestos workers. Tubercle 10:404-407. I Report of the Department Committee on Compensation for Industrial DiaMinutes of Evidence, Appendices and Index. London, Wyman and '"ft* a ' itA* . PRODUCED BY FORD