Document J38G8bX4RMw1vMD7mX0kXqJw6

dAaa -072.8 MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203 without tolyltriazole (VW160390). Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.90% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, and 0.1% sodium lauryl sulfate. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Microbios Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1991 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbios' Reagent. Test conditions: Diluent: Millipore Milli-QTM water. Test temperature: Not noted. Stock and test solution preparation: A primary 2000 mg/L stock solution was prepared by dissolving 200 mg in 100 mL Millipore Milli-QTM water. The pH of the stock solution was adjusted from 5.7 to 6.6 using 1.0 N NaOH. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL Cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss RESULTS____________________________________________________ Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L 000657 Element value: 5-minute EC5o = 1000 mg/L 15-minute EC50 = 550 (520 - 590) mg/L 30-minute EC50 = 320 (300 - 350) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test substance 30-minute EC50for Photobacterium phosphoreum was determined to be 320 mg/L with a 95% confidence interval of 300 to 350 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ This study was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number J1608-8 1991. OTHER______________________________________________________ Last changed: 6/27/00 000658 Ul 000659 5 I ENVIRONMENTAL LABORATORY > CL O O MICROBIOS MICROTOX SYSTEM I-- CO LU TOXICITY TEST RESULTS CQ PERCENT LIGHT LOSS J1608 7 /2 4 /9 1 BUTYLCARBITOL DUP0N0LME TOLYTRIAZOLE FOAMBT FLMER NOCmSULFATE FC203 TESTCONC. RM 8887 RM 3021 RM 27913 FM 4119 FM 3820 RM 32067 irfo TOLYTRIAZOLE ta L 1.25 3.min IS-min SQ-mln 8.min tS.ffilfi 3fl-m1n 8.in 18.min afl-mlwi .m tn |8.inln no-min 3.1i 20 41 54 ifl.mtn 8.mia 18.ila IB.HIWS.tnln ULBID 2.5 39 67 79 3.125 32 30 32 5.0 74 89 92 6.25 51 49 52 10 87 92 95 12.5 88 87 70 25 82 80 82 31.25 9.3 16 22 50 62.5 24 38 48 100 125 12 10 14 9.7 17 22 4.4 7.1 5.8 37 SS 67 8.8 19 26 2 50 17 19 19 10 18 20 13 17 23 64 67 76 17 30 42 5 00 31 29 31 12 18 21 19 24 31 30 49 64 1000 49 48 48 23 27 30 23 30 42 48 64 76 EC50 *1000 -1000 -1000 3.0 1. 1.1 1.1 .i 8.1 1000 1000 1000 1000 1000 1000 too Ito to -1000 Sto 330 95% C. 1. . JA* rum ft* im i) 08i ft***4| ftM-9441 |MBf BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY M1CRO8J0S MICROTOX SYSTEM RAW DATA LR <J )0>O&-> Pg | of H (\/UjliQ3TEST SUBSTANCE: F f - 1 u j*\> e xA T ^ I v + n a ^ k . 9o) TEST DATE: ANALYST: SAMPLE CUVETTE s\ C\ t*4.)L FINALASSAY CONCENTRATION BLANKCONTROL DUPLICATE INITIAL REAONG 1(0) Ty ST 5-MIN 1(5) 55~ 53 FINAL READINGS 15-M1N 30-M1N I (15) 1(30) 3 8 32. 37 3 1 BZ 7>5 SI t'K X'pliW e VH 27 3 1 5 X 33 25~ B3 9*50 loo 3<\ 2o c s >L;p)tCAie V Y7 27 IH BH $ 7 3 5 13 II CH 'ibupKcoCtiL 31 2o IX 35 ; <L5 / oot> V- 1 ^,:Vji : ^,:`yr '.' i' ; t>up||'f4e ; !o9. 23 35L H if 2 ? 8- Stock oln. prop: Initial pH: ~ . 7 loo m i M ilh '-o u-yJer- ~ 2 c o o r t i L - <\cX^(.OnJL ~ 3.o /v , % pH adjusted to L . b with 0 .1 A/ Osmotic adjustment: Added 'mg NaCI to l b mL sample Final appearance ' of ' test solution: ( jt ia C j 6 r r ^ s r U ^ ________ Comments: '' ' ________________________ 000660 BEST COPY AVAILABLE MICROTOX<r> DATA SHEET P C -2 0 3 w / o T o l v t r i a z a l e <VW 1 6 0 3 9 0 ) 5 - m i n e x o . J 1 6 0 8 - 3 7 / 1 9 / 9 1 s a b PAIR # CONC. 1 125.000 2 125.000 3 250.000 4 250.000 5 500.000 6 500.000 7 V. 1o o o . o o o 8 7.1000.000 Io /It 8 1 .0 / 44.0 9 9 .0 / 52.0 1 00 .0/ 48.0 9 4 .0 / 47.0 8 7 .0 / 35.0 9 4 .0 / 39.0 9 1 .0 / 2 8 .0 102.0/ 3 2.0 BLANK B o / B t = 9 2 / 54 BLANK RAT10= 0 . 5 8 7 0 G -O BS 0 .0 8 1 0 .1 1 7 0 .2 2 3 0 . 174 0 .4 5 9 0 .4 1 5 0 .9 0 8 0.871 EC 5 0 = 7 . 1 0 8 3 . 8 8 3 ( 8 9 4 . 2 0 6 TO X 1 3 1 3 . 7 9 3 ) CS o ^ lO O O R = 0 . 9 9 0 0 9 SLOPE = 0 . 9 1 4 0 IN TER C EP T = + 6 . 9 8 8 3 G -ES T U q ? L o S & f* 0 .0 9 6 0 .0 9 6 0 .2 0 2 0 .2 0 2 0 .4 2 6 0 .4 2 6 0 .8 9 5 0 .8 9 5 7 .^ 1o \% /S ' 31 A <\ H8 17 '0 6 ~ b i M cz> (1 6 -cb s) X 9p 11*/7 . ## RES. 1 0.200 2 0.161 3 0.114 4 0.128 5 0.123 6 0.118 7 0.182 8 0.178 o Vv 5 LOG CONC. t, . ............ . 'ii * -- >. A* -'-i/ MICROTOX is a Registered Trade Mark of Microbics Corporation'V " *1*K\*' 000661 BEST COPY AVAILABLE MICROTOX(r) DATA SHEET F C - 2 0 3 w / o T o l v t r i a z o l e (VW 160390) 15-<nin exo. J 1608-8 7 / 1 9 / 9 1 s a b PAIR # CONC. Io/It 1 125.000 8 1 .0 / 27.0 2 125.000 9 9 .0 / 33.0 3 250.000 100.0 / 29.0 4 250.000 9 4 .0 / 27.0 5 500.000 8 7 .0 / 18.0 6 5 0 0 .OOO 9 4 .0 / 20.0 7 7.1000.000 9 1 .0 / 14.0 8 7.1000.000 102.0 / 15.0 BLANK Bo/Bt= 92 / 38 BLANK R A T 10= 0.4130 G-OBS 0.239 0.239 0.424 0.438 0.996 0.941 1 .685 1.809 EC 50 = 551.535 < 517.055 TO 588.314 ) &SO- SSO (5X0 ~ 57D)nn^!L R =0 .99738 SLOPE G-EST L\M less*0 0.234 0.234 0.461 0.461 0 .907 0.907 ! .785 1 .785 h l<\ Jo JO Jo *3 i *1 '> e - c ^ KtCO _f *I n 3o H<\ kH ## . 1 2 3 4 5 6 7 8 RES. 0.092 0.092 0.064 0.063 0.064 0.062 0.090 0.094 PROB. 96 96 46 44 47 44 91 100 0> l y-\, i ; -I ' I. -1 > ' --2 - 6- 2-------------- - -4 - --3 . ';- 5 LOG CONC. ^iii ii L ii 0 <G aa aa G iA iM H A S I I I S -+ 7. ijMICROTOX is a Registered Trade Mark of Microbics Corporation, ?s%' 000662 BEST COPY AVAILABLE MICROTOX <r ) DATA SHEET F C -2 0 3 w/0 t o l y t r i a s o l e <VW 160390) 3 0 -in in e x o . J 1608-8 7/19/91 sab PAIR # CONC . lo / It 1 125.000 8 1 .0 / 2 1 .0 2 125.000 9 9 .0 / 2 5 .0 3 250.000 100.0/ 2 0 .0 4 250.000 9 4 .0 / 1 9.0 5 500.000 8 7 .0 / 1 1.0 6 500.000 9 4 .0 / 1 2.0 7 7.1000.000 9 1 .0 / 8 .0 8 7.1000.000 1 0 2 .0 / 8 .0 BLANK B o / B t= 92 / 32 BLANK RAT 10= 0 . 3 4 7 8 G -O B S 0 .3 4 2 0 .3 7 7 0 .7 3 9 0 .7 2 1 1 .7 5 1 1 .7 2 5 2 .9 5 7 3 .4 3 5 EC 50 = 3 2 4 . 2 8 7 < 3 0 3 . 6 2 7 TO 3 4 6 .3 5 3 ) ECS o - 3ZO (3 0 0 - 350) R =0 .99642 SLOPE 0 .9 2 7 9 INTERCEPT = + 5 .7 8 1 6 6 -E S T 0 .361 0 .361 0 .7 5 8 0 .7 5 8 1 .5 9 0 1 .5 9 0 3 .3 3 9 3 .3 3 9 'o Loss(0 X ^/ HK U* t*3 f 7 S 74, 77 {0 6 ^ A/ec> ^ / * 6-cbs) 4 R ES. PROB. 1 0 .1 1 3 100 2 0 .1 0 6 89 3 0 .0 7 2 41 4 0 .0 7 3 42 5 0 .0 7 5 44 6 0 .0 7 4 44 7 Ok 102 81 8 0.1 1 1 97 1> -V -- 6- CC ':{/*.`Y I O> 1I I * I 1* ' ^ --v ' ' ~ -- 4_ -1 4 2--r-rr-- u-" - v. S:. 3 . .LO S CONC. A_ 6 _ .. ..Vi- ... :'**.t$&i] y? . ; MICROTQX i s a R e g is t e r e d T ra d e Mark o f ' M i c r o b i c s C o r p o r a t i o n . L -8 - O --------7 < 6 6 A M M A S 00063