Document Gzv8RYqxOJ8ge6x3ZKnOJRXpN
I, E,
du Font de Neraours and Company
Haskell Laboratory For Toxicology and Industrial Medicine
AR226-2821
TEH-DOSE SUBACOTE ORAL TEST (RATS)
The test material (H-3824) was administered dally by stomach tube to each of six young ad
five t$iaes a week for two weeks, Groups of three test rats, along w Lth eont rols, weze sacri fleed foi
aceer tlie last treatment.
Results
a a
lw
Indlvl dual LiMrer Weight (R>
Aquftoua
4 Hours After 14 Days After 4 Hours
Saspo
Doae
10th Dose
10th I )ose
10th
%
(mg/kR/day) Mortality
Toxic Signs
Control Teat Control Test Contro-
5
220
0/6
Slight depression in rate 17.6 15,5 19.7 16.8
5.1
oanfdwreeicgohvt egryainpedriuordinsg test 16.7 15.3 17.0 16.0 5.0
16.1
18.8
16.3
17.8
4.8
0.5
22
0/5**
Very slight depression in rate of weight gain during test period
One animal accidentally killed with 8th dose.
17.6 14.7 19.7 16.0
5.1
16.7
14.5
17.0
16.3
5.0
16.1
16.3 18.4
4.8
&
ACUTE ORAL TEST (RABBITS)
Procedure: The test material (H-38-24) was administered as 3313% aqueous suspension by intragastric in albino rabbits in single doses. Survivors were killed i.0-l6 days later.
Results;
Dose (mR/kfO
5000
,3,4n0n0
2250 1500
Mortality 0/1
,,0/1,
0/1 0/1
Toxic Signs
Weight losses for 12 and 8 days, respectively, at 5000 and 3400 ing/kg; decreased f.,od and water consumption at these doses; urine
of a11 rabbits, first 24 hours,
clear, foamy and brown
Weight When Killed Body (e) Liver (e)
2189
135
2,,4,,86
3137
inion
.
170
g
Liver Wt
Body Wt
6.2 41.-14 5.4
ACUTE SKIN ABSORPTION TEST (RABBITS)
^.fSSS^SS.6: The teat material (H-3825), aa rppplwri, vaa flppllo^ In n1ng1a rfnnoa fn ^-he finely clippe rabbits and rubbed in lightly with a glass rod. The animals were restrained In wooden stocks for 24 ^ skin was washed with warm water. Survivors were killed 14 days later.
'5 Results:
B>
--------
^ Dose*** (mn/ta^
1'
3765
^ 2250
|
"OO
Mortality 0/1 0/1 0/1
Toxic Sl^ns
None
WelRht When Killed Body (e) Liver (s)
2869
122
2657
167
2734
88
Liver Wt "
,-
Body Wt
----
4.2 6.3 3.2
0
?
TEH-DOSE SUBACUTE SKIN ABSORPTION TEST <RABBITS>
ar
^
Procedure.:
^i^81'11for
The test material (H-3825), as received, was two week8* After 6at^ exposure. Which lasted
rubbed Into the clipped six hours, the material
akin of six male alb was washed from the
^ Three teat and three control rabbits were sacrificed four hours and 14 days after the laat treatment
a. ------------------.
^
g *** Based on active ingredient.
-
Reaulta:
Dose****
(mg/kg/day^ 1500
Mortality 0/6
Toxic Signs
None
**** Based on active ingredient.
Individual Liver Weights (g)
4 Hours After 14 Days After
10th Doae
10th Dose
Control Teat Control Teat
131
103
86
105
82
110
124
180
108
140
139
157
Individual L 4 Hours Afte
10th Dose Control Tes
4.6 4.2 3.6 4.6 4.3 5.2
Summary: L^^^^^3ihibited low cumulative oral toxieity for the male rat when administered repeated
22 ing/kg/day or 220 ing/kg/day. Except for a very slight adverse effect upon weight gain, there were toxieity. The livers of these test animals Kereeraaimetrically and histologically within the range
be contrasted with the results obtained vhea'flfffffuaa administered orally to rate at a rate of. 22
of these animals were extremely heavy and histologically abnormalC|^^H^IIHI^HHHHHI^B
7|J'--1^------^--^|Iias Low acute oral toxieity and low acute dermal toxieity for the male rabbit, the
Doses (ALD'e) being greater than 5000 mg/kg and greater than 3765 ing/kg, ^respectively. Vl--------H'as for the male rabbit when administered repeatedly via the dermal route at a dose of 1500mg7kg7d8y. L
body weight ratios were considered normal for all the rabbits examined; moreover, the livers did not e
which could be attributed to the administration of the test material.
o The difference between the response of the rat and that of the rabbit to acute oral doses
to liver weight suggests a species difference. Organs other than liver were not studied histologica
/ ^\U^f ^
HS/mfa
Date; May 6. 1964