Document Gz1REb14gMnn0dEb3agBK5Xnm

BACK TO MAIN TOXICITY TO FISH (Saltwater Rainbow Trout) TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder of uncharacterized purity. METHOD Method: Standard procedures for Testing Acute Lethality of Liquid Effleunts (Environment Canada, 1980) Type: Acute static, renewal after 48-hours GLP: No Year completed: 1985 Species: Rainbow Trout, Salmo gairdneri Fish source: Rainbow Springs, Thamesford Fish age at test initiation: not noted Fish acclimation to salt water: fish gradually acclimated to increasing salinity; held at 30 parts per thousand salinity 8 days prior to test initiation. Exposure period: 96-hours Analytical monitoring: Dissolved oxygen, pH, conductivity Statistical methods: Not noted. Element values were calculated for each replicate series, but not combined for the whole study. A cumulative mortality-concentration plot was used to estimate the LC50. Test conditions: Dilution water: Mississauga dechlorinated tap water amended with calcium, magnesium, sodium, and chloride to obtain 30 parts per thousand salinity Dilution water chemistry (initial): pH: 7.4 - 8.0 D.O.: 8.6 - 9.0 mg/L Conductivity: > 20,000 mhos/cm Stock solution preparation: 1000 mg/L Exposure vessels: Not noted; solution volume 35 L Number of replicates: 2 tests - run 4 days apart, not replicated Number of organisms/vessel: 6 Loading: 0.75 g/L Number of concentrations: 4 plus a blank control Water chemistry during the studies: Dissolved oxygen ranges 8.1 - 10.3 (control) 8.8 - 10.1 (30 mg/L) BACK TO MAIN pH ranges 7.6 - 8.2 (control) 7.3 - 8.0 (30 mg/L) Test temperature (0 - 48 hours): Photoperiod: 12-hours light, 12-hours dark 15C Element basis: mortality RESULTS Nominal concentrations: 5, 10, 20, 30 mg/L Element values (95% confidence interval) calculated per replicate: 96-hour EC50 = 13.7 (10.7 -17.7) mg/L 96-hour EC50 = 13.7 (10.7 - 17.8) mg/L Mortality of controls: 17% (1/6 in both studies) DATA QUALITY Reliability: Klimisch ranking = 2. This study satisfied all criteria for quality testing at the time performed, but actual concentrations were not measured. Results were based on nominal concentrations. Additionally, sample purity was not adequately characterized. REFERENCES This study was conducted by Beak Consultants Limited, Mississauga, Ontario, Canada for Panarctic Oils Ltd, Calgary, Alberta, Canada. OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 6/12/01