Document Gmyqnov7N014vpMb5Vompn4nx
normal c hr ucvi l 1 n and t tuchromatin from urothani- -t r<*aced rats was Ident 11 a 1 . The activity of HiV4' pit;' (NIL,) ;.S0>,-dependent RS'A polymerase activity of 1 no la ted lung nuclei was not affected by urethane injection. The two KHA poly
merase activities of liver nuclei were not Inhibited
bv th carcinogen. Mg?+-siimulated nuclear RNA
Carcinogenesis Abstracts Vol. 13, No. 7 July, 1975
i > > j J A GAS-CHROMATOGRAPHIC METHOD FOR THE ^ PREPARATION OF UC-LABELED VINYL CHLOR-
IQ. (Eng.) Wagner, E. R. (Dow Chemical Co7~
Midland, Mich. 48640); Muelder, W. M. ; Wntanabe, P. G. ; Hefner, R. K. , Jr.; Braun, W. H. ; Gohring, P. J. J. Ltiballcd Compd. 11 (4) : 535-542 ; 1975,
A method for producing vinyl-'1'^ chloride (!t*C-
VC) by the dehydrohalogenacion of 1,2-dlchinr-
ethane- 1
(lf,C-DCE) is described. 1 uC-VC is
produced as a gas mixed with helium by passing l4*C-DCE over a carbon catalyst at high tempera
tures. A modified Hewlett-Packard model 5750 gas chromatographic column is used. The large separa tion coil (2,4 m x 3 mm) Is packed wirh 80-90 mesh Dow Coming 410 Cum on Chromosorb W (acid washed). 'The smaller pyrolysis tube (90 cm x 3 mm) is pack ed with a charcoal mixture of 20 g Chromosorb W and 10 g Darco G-60 charcoal powder. An external thermocouple allowB independent heating of this portion of the column. The tube is connected to a thermal conductivity detector with a collecting syringe at the terminal end. The catalyst is heated to 470-480 C, and up to 10 pi of liquid
CARCINOGENESIS ABSTRACTS VOL. XIII
VlV0t FOR INDUCED CHROMOSOME ABERl WITH 13 COMPOUNDS (abstj
(Biol. Med. Res. Div.,
land); Grauwiler, J.
1975.
IDENTIFICATION CIN0GEN INDUCl ' -1ALIAN CELLS BY Nfl Evan*, C, H. (Si
3014); DiPaolo, j]
Res. 16:64; 1975.
3719
Noyes N.Y.
1975.
AFLATOXIN-1 NDlj CYTES IN ORGAN
W. F. (Roswell Pi 14203). Proa. Ami
3720
MALIGNANT TRAF MTX, AND BLE0F
Stract]. (Eng.) Bened;
Los Angeles, Los Angeles
S.; Cardner, A. Pnoc.
1975.
3721
NON-SPECIFIC
THESIS BY TUF
(Eng.) Poirier, M. C.
Md. 20014); Lieberomn,
aer Rea. 16:43; 197 5.
JULY 1975
tA'1 j ,
A..,.*, -C
Carcinogenesis Abstracts
Vol. 13, No. 7 July, 1975
i4C-DCE (3.4 mCi/mM) is injected into the tube; 14C-VC is collected in 1 ml aliquots. The efflu ent gas is bubbled into scintillation vials of Conciflour, 2-niethoxyethanol and toluene (1:1.8:11) in order to measure specific activity of the syn thesized gas. With a ga.3 flow of about 30 ml of He/min, the thermal conductivity detector revealed four peaks of activity. Analysis by infra-red spectrometry identified the peaks as impurities, HC1, 14C-VC, and unreacted l4C-DCE. Gross conta mination is avoided since the gaseous products exit at distinct intervals with little mixing. Further analyses by the gas chromatography and mass spectrometry showed that a minimum value of 46% (expressed on a mole basis) 14C-VC with a spe cific activity of 2.6 Ci/mM is generated from 5.5 vl 14C-DCE. The level of radioactivity of the *4C-VC declined over time (50% in nine hours). The polymerization of the gas on the walls of the collecting syringe is thought to be responsible for this. The mass spectrum of the synthesized 14C-CV was identical with that of the VC standard. This procedure yields a product with 96-97% purity. The amount of l4C--VC produced was limited by the amount of 14C-DCE, temperature, and He flow rate.
3717
THE MICRONUCLEUS TEST AS A SIMPLE MODEL,
IN VIVO, FOR THE EVALUATION OF DRUG-
INDUCED CHROMOSOME ABERRATIONS: COMPARATIVE STUDIES
WITH 13 COMPOUNDS [abstract], (Eng.) Matter, B. E.
(Biol, Med. Res. Div., Sandoz Ltd., Basle, Switzer
land); Grauwiler, J. Mutat. Rea. 29(2):198-199;
1975.
3718
IDENTIFICATION OF IN VITRO CHEMICAL CAR
CINOGEN INDUCED NEOPLASTIC TRANSFORMATION
OF MAMMALIAN CELLS BY NONIMMUNE LEUKOCYTES [abstract].
Co
I
3727
REDUC IN T