Document GmrqMrnBr4DaeKQNJvzrzk687

M A S 1 6 -O S 4 4 TO XIC ITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-602. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FC-602. Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 61.05% water, 20% ethylene glycol, 2% Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 1% magnesium sulfate, 0.7% xanthan gum, 0.1% starch, 0.1% n-(3-chloroallyl)hexaminium chloride, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ M ethod: NS-EN ISO 10253,1998: W ater quality - Marine algal growth inhibition test Type: Acute Static GLP: No Y ear com pleted: 2000 Species: Skeletonema costatum Source: Strain NIVA BAC1, stock culture maintained at RF - Rogaland Research, Stavanger, Norway. Elem ent basis: Estimate biomass by measuring fluorescence. Exposure period: 72 hours S tatistical Methods: Growth rates by Log linear regression on biomass (fluorescence) vs. time, as described in NS-EN ISO 10253. Calculation of NOEC done by single factor ANOVA followed by Bonferronis procedure. A nalytical m onitoring: pH T est Conditions: Algal N utrient Medium: Filtered natural seawater (34 ppt salinity) from an unpolluted site in Byfjord, Norway, enriched with nutrients according to ISO 10253 and recommendations from the Paris Commission. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 8.4. Stock and te st solution preparation: A 102.4 mg/L primary stock solution was prepared by diluting 0.1024 g of test substance in 1 L of growth medium. 002160 Aliquots were then added directly to growth medium in test vessels to create test solutions. Exposure vessels: 100 ml flat-bottomed flasks containing 50 ml test solution. A gitation: Shaken continuously on an orbital shaker. Num ber o f replicates: 3 Initial cell loading: 2.0 x 103 cells/mL Num ber o f concentrations: six plus a blank control Lighting: Continuous fluorescent lighting at >50 pE/m2 sek. W ater Chem istry: pH range (0-96 hours): 8.3 - 8.4 (control exposure) 8.3 - 8.4 (102.4 mg/L exposure) Test tem perature range: 20 2 C RESULTS_________________________________________________________ Nom inal concentrations: Bk control, 0 .3 2 ,1 , 3 .2 ,1 0 , 32, and 102.4 mg/L: Reference substance (3,5-dichlorophenol) at 1.5 mg/L. A lgal Growth Response ErC values (based on grow th rate) 72-hour ErC5o = ~78 mg/L 72-hour NOEC = 32 mg/L Element values are based on nominal concentrations. R eversibility o f Growth Inhibition: Not conducted. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical components alone. C O N C L U S IO N S _________________________________________________ FC-602 exhibits a 72-hour ErCso of ~78 mg/L. The 72-hour No Observed Effect Concentration (NOEC) is 32 mg/L for growth rate. Validity criteria fulfilled. Control cell density increased by a factor of more than 16 in test period and reference substance was within normal range. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________ 002161 Reliability: Klimisch ranking - 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and it lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-3, 2000. O T H E R ____________________________________________ Last changed: 6/26/00 002162 L9.9u31.r2e-j3.no.; Page 1 o f31 (Ql AKKRNEOORfTSEKRING Nr. M i) ROGAIAND RESEARCH "FC-602 Light Water Brand A TC plus" Toxicity tests Report RF-2000/003 Lab. ref. no.: 99312-3 No. of pages: 31 ISBN: Author: Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 / 07.01 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-602 Light Water Brand ATC plus": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h ECm 48 h LC* 10 d LCK (mg/1): ~78 (mg/l): 200 (mg/kg dry weight): >3070 The results relate to the product as supplied by Novatech. Key-words: ' ~~ Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this document may be reproduced in any form without permission in writing by RF Rogaland Research Q. ! S tudy director J -l/M Inger Lisa Andersen for RF - Rogaland Research K&re Netland 002163 ANGER: Professor Otov Honssensvei 15, P.O. Box 2503 Ufhndhoug, 4091 Stavanger, Norway. Tel.: +47 51 8750 00. Fox: +47 51 87 52 00. Enterprise number.: 950884800. ROM' Tt>nmv4<Lnnt XX xSsO0---------- -------------. .At XXKA -JOXOe------------.At * to AO L9a93b1.r2e-f3.no.: Page 2 of31 CONTENTS: 1. Pr e fa c e ................................................................................................................................................................... 3 2. Test for toxicity w ith Skeletonema costatum........................ .................................................... 4 3. Test for toxicity w ith A cartia tonsa......................... .......................................................................10 C4 . Te st f o r t o x ic it y w it h orophium volutator..........................................................................15 5. Da t a s h e e t s ) .................................................................. ........,..................................... ...................................20 002164 L9a9b3.1r2e-f3.no.: Page 3 o f 31 PREFACE: ENcoovtaotxecicho.loTghiceaslatmesptslewiseraecpoenrfcoernmtreadteotnha"Ft Cn-o6r0m2aLlilgyhwt Willatbeer uBsraenddinATaC3%pluAs"FFinFa/gr6e%emAeRnt with solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995 /S F T 1998). pTehrefoprrmojeedctbwyaRsFca- rMriieljdeolaubt.aSt tRudFy-dRiroegcatolarnwdaRseSseenairocrhEfonrgi3nMeeNr Ionrggeer. All the tests were Lisa Andersen. bTyheNtoevsattreecshu.lts arepresented in separate sections. The results relate to theproduct as supplied This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). eTqhueipamccernedt iatnatdiownosrtkaintegsptrhoactetdhuerlaesbfoorrataolrlythmeeteetssttmheetNhoAdrsecqounirtaemineendtsinctohnecaercncrinedgictaotmiopne.tence, It also states that the laboratory has a satisfactory quality assurance system, which includes internal quality controlschemes, participation in interlaboratory comparisons, and the use of certified reference materials and secondary reference materials. The testsperformed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without thepermission in writing by RF - Miljelab. 002165 L99a3b1.r2e-f3.no.: Page 4 o f31 Test for T oxicity Marine alga: Skeletonema costatimi 002166 L9a9b3.1r2e-f3.no. Page 5 o f31 TEST REPORT Grow alga tShkienlheitbointieomn taecstoswtaitthutmh e m a r in e (Si fifDCK AKKREDITERING Nr. POI# ROGALAND RESEARCH TEST SUBSTANCE: "FC-602 Light W ater Brand ATC plus" TEST RESULT: The 72 hours EC50was calculated to ~78 mg/1. The test substance was supplied by Novatech, and the test results relate to this product TEST DETAILS: Method: NwSit-hESNkeIlSeOton1e0m25a3c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-ctMylaurminetraiclgoamlugtruomw.th inhibition test i | | TEST ORGANISM: Skeletonema costatum, strain NIVA BAC 1. organism is kept at RF - Rogaland Research. A stock culture of the test A if. PREPARATIONS: Nasabtuarsaisl sfeoarwthaetegrr(oswaltihnimtye:d3iu4m%.o)TfrhoemwaanterunwpaosllfuiltteedresditethirnoBugyhfjoarGdFw/Casfuilsteerd, and briefly heated to about 75C prior to use. The seawater was enriched with nCuotmriemntisssaiocnco. rding to ISO 10253, and recommendations from the Paris Test solutions were prepared from a stock solution. A stock solution of 102.4 mg/1 was prepared by stirring 0.1024 grams of the product in 1 litre of growth medium. Other concentrations were prepared from this stock solution. INCUBATION: Test cultures o f 50 ml were incubated in 100 ml flat-bottomed flasks on an orbital shaker, 3 replicates for each concentration. Test cultures were inocu Tlahteedtetostawbaosutru20n0u0ndceerllsc/omntlifnruoomusanligehxtp.(o>ne5n0tipaElly/nmr-osewki)nfgroinmocfululuomresccuelntut re. tubes o f universal natural white type, at a temperature of 20 2C. Tbthyheeftlteuesostrtfelwascaskesnsrcueean.cfhpoHrdaambyoefuaostru7gr2reomhwoetunhrtssm,waenaersduesrpueebmrsfeaonmrtmsp.elAedslagosafli5ngdmrioclwawttehedrweoatnaskdmaenteaafssruhormeeedt. ITDANGER: Profess Olav Honsiensvi 15, P.O. Box 2503 Ullondhoug, 4091 Stavongor, Norway. U : +47 51 7 on 002167 L99a3b1.r2e-f3.no Page 6 of 31 DATA ANALYSIS: Calculations: vGerroswusthtirmatee,saws edreesccrailbceudlaitnedNbSy-EloNgIlSinOea1r0r2e5g3r.ession on biomass (fluorescence) ErCso: Tceohffneeccientnhctoirbnaicttoieornnytroeafftfiaeocpntrwoodnaustchcteaglgcirvuoilnawtgtehd50ob%fySirkneetdleeurtpcotonioleanmtiooanfcgboresottawwttuehemnrai3ste2em(xEpgrrC/le5sa0sn)e.ddTahsisthe 102.4mg/l.. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by singlefactor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: COMMENTS: VALIDATION: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance E,Cjo ErCjo EAo "FC-602 Light Water Brand ATC plus" - -78 >102.4 NOEC: 32 GVVaaralluupeehssPianarddeiIccnaaStlectauctloa3nt.e0cd.enbtyraintitoenrspogliavtiinogn.10N%O, E5C0%araencda9lc0u%laitnedhibbyitiuosninogftghreowcothmrpauttee.rpUrongitrsaimn mmeg/l. The results are based on growth rates for the time span 0 - 72 h, the period during which the growth is considered to be exponential in the control cultures (see growth curves). The validity criteria given in the test protocol are fulfilled, in that the control cell density increased by a factor o f more than 16 in the test period (corresponding to a growth rate of more than 0,9 d'1), the control pH did not vary by more than 1,0 units during the test and the 72 hours test results with 3,5-dichlorophenol was in the range normally found. Lab. ref. no.: TSaemst ppleerrioedc:eived.: Report date: 99312-3 22-2205..0191..9999 07.01.2000 Elin Horve AnalytEicnaglintecehrnician SeniAorgRe eMseoalvrcehrsSmcyierntist Quality Assurance Enclosed: Data sheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaulatbthoerapteorrmy aiscsciorendiintedwrbiytinNgobrwyeRgFia-nMAiclcjoreladbit.ation (NA). The report may not be reproduced in any 002168 L9a93b.1r2e-f3.no.: Page 7 o f31 PRIMARY DATA: Growth inhibition test with Sketetonem a costatum Test substance: T C -602 Light Water Brand ATC plus" Lab. ref.: 99312-3 Start date: 22.11.99 Cone, unit: mg/l Time: 22.11.99 23.11.99 24.11.99 25.11.99 Concentration 13:40 11:50 13:20 14:45 0 0.06 0.32 1.5 8.2 0 0.06 0.23 1.7 9.3 0 0.06 0.46 3 11 0 0.07 0.46 2.15 11 0 0.07 0.35 1.9 9 0 0.12 0.4 2.35 11.5 0.32 0.07 0.42 2.4 11.5 0.32 0.09 0.55 2.5 12.5 0.32 0.07 0.5 2.65 12 1 0.08 0.66 3.4 14.5 1 0.07 0.64 3 13 1 0.09 0.5 2.7 12.5 3.2 0.08 ' 0.58 3.4 15 3.2 0.09 0.45 3 13 3.2 0.09 0.38 2.95 13 10 0.1 0.35 3.1 15 10 0.1 0.43 3.1 14.5 10 0.07 0.43 2.6 14 32 32 32 102.4 102.4 102.4 0.09 0.5 2.7 14.5 0.08- 0.46 2.1 11 0.09 0.43 2.9 13 020.1 0.13 0.43 0.07 0.08 0.18 0.43 0.09 0.1 0.2 0.73 Growth pH pH rate Corre- at start at end day-1 lation of test of test 1.61 1.66 1.71 1.66 1.59 1.50 0.999 0.999 0.992 0.997 0.999 0.999 8.4 8.3 1.68 1.62 1.69 0.998 0.998 0.996 8.4 8.2 1.71 0.993 1.72 0.991 1.62 0.999 8.4 8.2 1.72 0.996 1.63 0.998 1.63 0.998 8.4 8.2 1.65 0.997 1.63 0.999 1.74 0.999 8.4 8.2 1.67 0.999 1.62 0.998 1.63 0.999 8.4 8.3 0.48 0.60 0.69 0.979 0.971 0.944 8.4 8.3 Reference substance (3,5-dichloroohenoD: 1.5 mg/l 0.1 0.16 0.48 1.5 mg/l 0.07 0.18 0.44 1.5 ma/l 0.1 0.15 0.38 Mean values: 0 0.32 1 3.2 10 32 102.4 0.07 0.08 0.08 0.09 0.09 0.09 0.09 Reference subst. 0.09 0.4 0.5 0.6 0.5 0.4 0.5 0.1 0.16 2' 3 3 3 3 3 0 0.4 1.1 1.4 1.15 10 12 13 14 15 13 1 1.2 0.79 0.98 0.80 0.993 0.999 0.987 8.3 8.2 1.62 1.66 1.68 1.66 1.67 1.64 0.59 0.86 0.999 0.997 0.995 0.998 0.999 0.999 0.964 The result of the reference substance (53% inhibition) is 0816 0.996 (20-80%) L99a3b1.r2e-f3.no.: Page 8 o f 31 GROWTH CURVES: Growth inhibition test with Ske/etonem a costatum -602Test substance: "FC Light Water Brand ATC plus" Lab. ref.: 99312-3 I* ViA 002170 L9a9b31.r2e-f3.no.; Page 9 of31 I n h i b i t i o n of the growth of S k e l e t o n e m a c o s t a t u m with: mg/1 (FC-602 Light Water Brand ATC plus) Experimental data: Concentration in Growth rate FC-602 Light Water Brand ATC plus Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean 0.32 0.32 0.-32 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 102.40 102.40 102.40 1.61 1.66 1.71 1.66 1.59 1.50 1.62 1.68 1.62 1.69 1.71 1.72 1.62 1.72 1.63 1.63 1.65 1.63 1.74 1.67 1.62 1.63 0.48 0.60 0.69 Inhibition in percent 0 0.0 0.1 0.0 - 0-1 0-0 0.0 00..00 0.0 0.0 0.1 0.0 70.4 63.0 57.5 002171 L99a3b1.r2e-f3.no.: Page 10 o f31 T est for T oxicity Marine herbivore: Acartia tonsa 002172 L99a3b1.r2e-f3.no.: Page II o f31 TEST REPORT a c u te toxicity test w ith the MARINE COPEPOD A c a f t i d tOItSCl (9) NORSK AKKREOITERING Nr.TOl* ROGALAND RESEARCH TEST SUBSTANCE: "FC-602 Light W ater Brand ATC plus" TEST RESULT: The 48 hours LC50was calculated to 200 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ISO/DIS 14669 - 1997: Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). TEST ORGANISM: Acartia tonsa University o f C(Doapnenah),asguepnp. lied by the Marine Biological Laboratory, CULTIVATION: The test organisms were supplied as eggs, and were cultivated in accordance wanitdhatdhuelt"sPwareirseCuosmedminissthioent-esRt.ing Test Protocol" (VKI). Large copepodites PREPARATIONS: Npasraibtourarstaiosl usfeosarew.thaetedri(lusatiloinnitwy:at3e4r.%To)hferowmataernwunaspofillltuetreeddstihteroinugBhyafjGorFd/Cwafisltuersed Test solutions were prepared from a stock solution. A stock solution of 1000 mg/1 was prepared by stirring 0.9998 grams o f the product in 1 litre of dilution water. Other concentrations were prepared from this stock solution. INCUBATION: T2v5ihaelmstlewsoetfrwteeakssterpsuotnluuintnidoegnrlaaasns1d6c5ohn/a8tnahiimnliegarhlsst/,(d24a8rrkemppllihrcooalttloeepsdefrroiirmodeavacitahalsc)toeenmaccpehnertcraoatuntirtoaenion. fi:nTghe2d0ata s1heCet.. pH and oxygen measurements were performed as indicated on the Professor Qfov Hnrm*nviM ^*0? 1 002173 L9a9b3.1r2e-f3.no.: Page 12 o f31 DATA ANALYSIS: The effect on the survival of Acartia tonsa is expressed as the concentration of paeprrtoedsutcctolnecthenatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLioCn5.0)T. hTeh2e4cohmoubrisnaenddm4o8rhtaoluitrys LstCan5d0avradlupersocaenddutrheeP9R5%OBcIoTnAfidneanlycseisli,mNiat twioenrael cSawlceudlaisthedEbnyviurosinnmg etnhteal Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single cfaocntcoernAtrNatOioVnAmfeoalnlowwiethd tbhyeBcoonntfreorrlomneisanp.rocedure for comparing each Test results: Table 1. LC and NOEC values obtained with "FC-602 Light Water Brand ATC plus" Acartia tonsa 24 hours 48 hours LC10 120 (56-190) 87 (42-130) LC50 350 (250-490) 200 (140-270) LC90 970 (650 - 2200) 450 (320-900) NOEC: 100 100 ctVhaaellcucueolsmaitnpedduitbceyraptPerRo1Og0r%BaIm,T5m0a%enaGlayrnsadips,9kPa0%naddl9Ien5thS%atalcittoy3n.a0fif.dteern2ce4 ianntedr4va8lhsoaurres.indUicnaittsedin. mNgO/lE. CVaarlueecsaalcreulated by V a lidatio n: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the test was greater than or equal to 4 mg/l, the percentage mortality in the controls was less than 10%, and die 48 hours LC50 of 3,5-dichlorophenol (reference) was in the range normally found. LSaamb.prleef.rnecoe.:ived.: TReesptoprterdiaotde:: 2027-22.0051..01.2910..990990 Elin Horve Age Molversmyr Engineer Senior Research Scientist Analytical technician Quality Assurance' Enclosed: Datasheets Ie,dwbityhoaulatbthoerapteorrmy aiscsciroendiintedwrbiytinNgobrwyeRgFia-nMAiclcjoreladbit.ation (NA). The report may not be reproduced in any 002174 Lab.rcf.no.: 99312-3 Page 13 of31 PRIMARY DATA: Acute to xicity test w ith A cartia tonsa T est substance: "FC-602 Light W ater Brand ATC plus" Lab. ref.: Start date: Measurement: 99312-3 10.11.99 Number of dead Concentration (mg/l) Control 10 32 100 320 1000 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 5 56 30 4 5 5 5 55 20 5 5 55 20 5 5 55 20 5 6 54 20 5 .564 20 5 NUMBER OF DEAD 24 hours 48 hours ACE BDF Total ACE BDF Total 0 00 001 000 0 0 00 1 100 0 1 00 10 1 000 0 0 02 00 2 0 10 1 0 20 21 3 4 10 4 43 2 7 4 15 5 54 5 5 64 19 5 20 Reference substance (3,5-dichIorophenol): 1.0 mg/l 5 55 1 10 331 30 645 001 3 0 11 The result with the reference substance is within the range normally found. 9I L9a9b3.1r2e-f3.no.: Page 14 o f31 Test substance: Lab. ref.: Start date: "FC-602 Light W ater Brand ATC plus" 99312-3 10.11.99 Measurem ents o f oxygen and pH . Concentration (mg/l) Start Oxygen (mg/l) pH 24 hours 4 hours Oxygen pH Oxygen (mg/l) (mg/l) Control 7.3 8.2 7.4 _. 10 7.6 8.2 7.3 32 7.6 8.2 7.2 100 7.6 8.2 7.1 320 7.6 8.2 6.7 1000 7.4 8.2 5.7 pH 8.2 8.2 8.2 8.2 8.1 8.0 Reference substance (3,5-dichlorophenol): 1.0 mg/l 7.3 8.2 7.4 8.2 L99a3b1.r2e-f3.no.: Page 15 o f31 j T est for toxicity Marine Amphipode: Corophium volutator IiI i i 002177 L99a3b1.r2e-f3.no.: Page 16 of 31 TEST REPORT ACUTE TOXICITY SEDIMENT TEST WITH THE MARINE AMPHIPOD C o r o p l l i u m V O lu td tO Y (0) NORSK AKKREDITERING Nr.Poll ROGALAND RESEARCH TEST SUBSTANCE: "FC-602 Light W ater Brand ATC plus" TEST RESULT: The 10d LC50 was calculated to >3070 mg kg'1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ATSoxTiMcityET13e6st7s-9w0it,hStManadrainredaGnudidEestFuoarriCneonSdeudcimtinegnt1s.0 day Static Sediment TEST ORGANISM: Corophium volutator, from an unpolluted site near Trondheim . CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in afeeerdatinedg.seawater at 15C. A small amount o f natural seaweed was used for PREPARATION: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjorden outside Stavanger was used for dilution water. Sediment collected at an unpolluted site was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test. Each test concentration was prepared by adding the product in 600 grams of damp sediment in a plastic bucket. 200 ml o f seawater was added and the bucket was shaken for 3 hours. The sediment was divided into three beakers, 400 ml of seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample of tchaelcdualamtepdsaeds immgenktgw'1assedainmaleynsteddrfyorwweiagthetr. content, and all concentrations are INCUBATION: The test was run in glass containers (600 ml) each containing 200 g o f test sediment, app. 400 ml of seawater and 10 animals, 3 replicates for each concentration. The beakers were kept under continuous aeration at a tinemdipcearteadtuorenothfe15data1shCe.etp.H and oxygen measurements were performed as 002178 +A7^G R : Professor O lav Hamsensvei 15, P.O. Box 2503 Ullandhaug, 4091 Stavanger, Norway. Tel.: +47 51 8 7 5 0 00. Fax: +47 51 8 7 5 2 00. Enterprise number.: 950884800. R W ifN ' TttfM'nvJiiawmt w v ifW iWrMkn Kionunv TrI ' 55 54 38 50. Fox.* +47 55 54 38 60 L99a3b1.r2e-f3.no.: Page 17 o f31 DATA ANALYSIS: CALCULATIONS: The effect on the survival o f Corophium volutator is expressed as the concen vmtraaolturioteanaliontydf patehprerto9ed5s%ut cctocnloecntehfnaidtlretanotci5oe0nl%iwmeoirtfeatrhueesectdaeslfctouarlnacitameldcaublslya(tLuiosCnin5.0g)T.thhTeehs1et0acndodamayrbdLinCe5d0 PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-602 Light Water Brand ATC plus". Corophium volutator 1 0 days LCio 1750 LCjo >3070 LC90 >3070 NOEC: 3070 GVVaaralluupeehssPianarddeiIccnaaStlectau1tl0a3%t.e0.d. 5b0y%PRanOdB9I0T%anlaetlyhsailsi.tyNaOfteErC1a0rdeacyas.lcuUlantietsdinbymthgekcgo'1msepduitmerepnrtodgrryamwemigeht. V alidatio n: The validity criteria given in the test protocol are fulfilled, in that the dissolved otFhxleuyopgreearnnccteeonnnteacge(renetmfreaortreiotnancleiat)ytwtihnaestheinnedtchooenftrtrahonelgsteewsnetorwremalseasglslrytehafoateunrn1tdh5.%anTahonredesqeadutiaemlstteownt6itu0hs%ed, irsecfooumnmdetnodbeedwhiitghhinera.ccepted limits for organic content but the silt fraction is Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0 .8 LSaamb.prleef.rnecoe.:ived.: TReesptopretrdiaotde:: 99312-3 0037-21.0021..01.2920..990990 W vL. AnalyEEtliicnnaglHintoeecrevhrenician Age Molversmyr Senior Research Scientist Quality Assurance Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhaoulatbthoeraptoerrmy aiscsciroendiintedwrbiytinNgobrwyeRgJiFa-nMAiclcjereladbit.ation (NA). The report may not be reproduced in any O0 2 V9 L9a9b3.1r2e-f3.no.: Page 18 o f31 PRIM ARY DATA: Acute to xicity test w ith Corophium volutator T est substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: Measurement: 99312-3 03.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 10 10 10 257 30 10 10 10 478 30 1050 10 10 10 30 3070 10 10 10 30 NUMBER OF DEAD 5 days 10 days ACE B DF Total ACE BDF Total '0 0 0 0 00 0 1 00 1 0 10 1 3 10 4 2 01 3 Reference substance (fluoranthene): 20.3 10 10 10 30 3 22 The result with the reference substance is within the range normally found. 7 002180 L99a3b1.r2e-f3.no.: Page 19 o f31 Test substance: Lab. ref.: Start date: "FC-602 Light W ater Brand ATC plus" 99312-3 03.12.99 Measurements o f oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 91 8.1 257 93 8.1 92 8.1 478 93 8.1 91 8.1 1050 95 8.1 92 8.0 3070 96 8.1 88 7.9 10 days Oxygen (%) pH 91 8.0 82 8.1 81 8.1 90 8.1 89 8.1 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 | 002181 99312-3 Data Sheets i 002182 99312-3 HMS-DATA3LAD (Heise-, Miijo- og Sikkerhetsdatablad) Dokumentnr.: Srscatter : 07-2573-9 Utgitt : 10/11/93 Versjon : 05.00 08/10/97 Skrevet ut : 11/11/98 Side 1 av n HMS-datablacer er utarbeidet av Miljeavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIXASJON AV KJSMIXALIET OG ANSVARLIG FIRMA. Importer: 3M Norge A/S Handelsaavr.: FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) Deklarasjor.snummer, Procuktregistered: Telefon, Gifdinfornesjonssenrraien: 22 59 13 00 2 OPPLYSNINGER OM KJEMISK SAMMENSETNING. Informasjon om enkeltkompcr.enter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VEXT-PROSENT VANN 7732-18-5 2-(2-3UTOKSYSTOKSY)-ETANOL / 112-34-5 (DIETYLSNGLYXOLMONOBUTYLETSR.) Faresymboler/risikosetninger: Xi; R: 36 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 AMFOTER FLUOROALXYLAMID-DERIVAT Trade Secret ALKYL-SULFAT-SALTER Trade Secret TRIETANOLAMIN PERFLUOROALXYL-SULFONAT-SALTER RESTER AV ORGANISES FLUORKJEMIKALIER 102-71-6 Trade Secret Trade Secret 1 o 35.0 - 39.0 17.0 - 21.0 S 14.0 5.0 - 10.0 16.0 =* 3.0 1.0 - 5.0 Ikke kjent 99312-3 FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) 3 VIKTIGSTS FARSMOMENTSA. Viktigste skadevirkninger Helsefare: IRRITERENDE (Xi) Irritrer aynene. Sikkerhet: Miljafare: 4 F0RSTSKJ ;l p s t i l t a x . Tiltak ayr.e: Skyll straks aynene grundig raed rikelige mengder vann i minst 15 minutter. Kontakt lege umiddelbart. Skylling under transport. Tiltak hud:Skyll straks huden raed spe og rikelige mengder vann. Fjern tilsalt tay. Hvis syraptomer oppstar, kontakt lege. Vask tilsalt tay far det brukes igjen og kst tilsalt skotay. Tiltak intending: Hvis syraptomer oppstr, bring vedkommende oort fra eksponeringskilden. Sarg for frisk luft. Kvis syraptomer.e vedvarer, kor.takt lege. Tiltak svelging: Kontakt lege umiddelbart. Fremkall brekninger kun i samrd med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstles person. 5 TILTAK V2D 3RANNSLUKNING. Egnet brannslukningsraiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: 99312-3 FC-201AF LIGHT WATER (TM) 1% AFFE (FREEZE PROTECTED) Forbrenr.ir.gsprodukter ved brann: Prosecvrer vec slukking av brann: 6 TILTAX VED UTILSIKTET UTSLIP?. Personlige vernetiltak: Se vernetiltak under andre punkter i dette HMS-databladet. Tiltak ved utilsiktet utsiipp: Ventiler omrdet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale. Spill samles opp umidd"elbart. Rengjer omrdet med vann. Plasseres i en egnet behoider med lokk. Fjernin av kjemikalieavfall: Se pur.kr 13, Fjernir.g av kjemikalieavfall. 7 KNDTERING OG 0PP3EVARING. 3etingelser for sikker oppbevaring: Mit ikke lagres i omrlder hvor produktet kan komme i kor.takt med mat eller legemidier. Lagres ved temperatur under 49 grader celsius. Lagres ved temperaturer over 0 grader celsius. Hold beholderen tett lukket n4r produktet ikke er i bruk. Uforenelige materialer: Ventilasjon: Opp'oevar emballasjen p et godt ventilert sted. Sikkerhetstiltak for 4 forhindre brann: Sikkerhetstiltak for 4 forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for 4 forhindre statisk elektrisitet: ForhoIdsregier ved bruk: Hold emballasjen tett lukket. Royking forbudt: Under bruk av produktet kan reyking forrsake forurensning av tobakken og/eller royken og farlige dekomponeringsprodukter kan dannes. Se punkt 10, stabilitet og reaktivitet, skadelige dekomponenter. u99io3.1r2ej-.3no.: rge T uj FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) 8 EKSPONERINGSXONTROLL OG PERSONLIG VERNSUTSTYR. Oyevern: Unngi & fi dang, spray eller tike i oynene. Bruk tette vernebriller med ventiler. Hir.dvern: 3ruk egnede verr.ehar.sker avhensiktsmessigmateriale ved hir.dtering av produkcet. Vernenansker av folgende materiale(r ) anbefales: Butvlgummi. Annet hudvern enn hir.dvern: Unngi hudk'ontakt. j ! \ i [ IiI i ndecrettsvern: Unngi inninding av lufzbiret materiale. 3asert pi konser.trasjonen av luf tforurensnir.ger i arbeidsarmosfaren veiges en av folgende type(r ) godkjent indedrettsvern: halvmaske med filter mot organiske damper og vsskeformige partikler: A/P2SL. Svelging: Det mi ikke spises, drikkes eller roykes under arbeid med produktet. Vask utsatte omrider grundig med sipe og vann. Vask hendene etter hindtering og for spising. Anbefalt ventilasjon: Kvis utsugningsventilasjon ikke er tilstrekkelig, mi egnet godkjent indedrettsvern benyttes. Sorg for tilstrekkelig ventilasjon slik at dampkonsentrasjonene holdes under anbefalte administrative normet og/eller kontroller spray eller tike. i ; | ij I j j 1 ! t : j Adm. normet for forurensning i arbeidsatmosfare, Februar 1996: VANN (7732-L8-5) Anmerkninger.: Ingen adm. norm. 2-(2-3UTOKSYETOKSY)-ETANOL / (DISTYLENGLYX0LM0N03UTYLETER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdianbefalt av produsenten. (Ingen norsk adm. norm). 1,2-ETANDIOL (107-21-1) 8-timers verdi ppm: - (stov) 8-timers verdi mg/m3: 10 (stov) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) | I j t i | | !. ,AMFOiSR FLUOROALKYLAMID-DERIVAT (TradeSecret) Anmerkninger: Ingen adm. norm. j j ALKYL-SULFAT-SALTER (Trade Secret) Anmerkninger: Ingen adm. norm. 99312-3 FC-201AF LIGHT WATER (TM) It AFFF (FREEZE PROTECTED) rfri2TAlt0LAM I!I-- (10 I-tt- ct)---------------------------8-timers verdi ppm: 8-timers verdi mg/m3: S PEAFLUOROALXYL-SULFONAT-SALTSR (Trade Secret) 3-timers verdi mg/m3: 0,1 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). RESTER AV ORGANISXE FLUORXJEMIXALIER (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. 9 FYSISXE OG KJEMISXS EGENSKAPER. Tiistandsform, farge, lukt: Klar ravfarget vasske. pH: 7.5 - 8.5 Xokepunkt/-intervall: * 100 C (3egynr.ende kokepunkt) Smeitepunkt/-intervall: Ikka relevant Flammepunkt: > 100 C (Miniflash) Selvantennelighet: Ikke relevant Eksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, o-vre: Ikke relevant Damptrykk: - 16 mmHg (Xalkulert v/20 C) Loselighet i vann: Blandbar Spesifikk vekt: - 1.10 (vann*l) Damptetthet: f Flyktige organiske forbindelser (VOC) 1.0 (luftl) (Xalkulert v/20 C) - 359 g/1 SCAQMD (g/1 av produktet) Fordampningshastighet: < 1 (butylacetatl) Viskositet: 18 centipoise 00218 Lao.rej.no 99312-3 i tA g C. W/ ^ * FC-201AF LIGHT WATER (TM) It AFTE (FREEZE PROTECTED) IO STA 3ILITST OG REAXTIVITET. Forhold som skal ur.ngis : Materialer som skal unngis: Ikke kjent. Farlige spaltningsprodukter: Karbanmonoksid og karbondioksid. HydrogenfLuorid. Giftige damper, gasser eller partikler. Termisk dekomponering av bruksferdig blanding forventes ikke . reprsentere noen risiko. Stabilitet og reaktivitet: Srabilt. Farlig polymerisas jor. vii ikke forekomme. 11 OPPLYSNINGER OM HSLSEFARE. Effekter eyne: Moderar ayeirritasjon: symptomer kan vare radhet, hevelse, smerter, trer og uklart syn. Effekter hud: Moderar hudirritasjon (etter langvarig eller gjentatt kontakt): symptomer kan vare radhet, hevelse, klae og tarrhet., Langvarig eller gjentatt eksponering kan fare til: Allergisk hudreaksjon: symptomer kan vare radhet, hevelse, biemmer og klae. Effekter innnding: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fare til: Pvirkning av sentralnervesystemet: symptomer kan vare hodepine, arhet, sovnighet, manglende koordinasjonsevne, nedsatt reaksjonsevne, slaret tale, svimmelhet og i ekstreme tiifeller bevisstlashet- Irritasjon (avre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfart med organiske fluorkjemikalier, som finnes i dette produktet, viser effekter som leverfforstyrrelser, vekttap, manglende appetitt, slavhet samt effekter som pivirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nir de brukes som forutsatt og i henhold til L9a9b3.1r2e-f3.no.: Page 2/ 0} Si FC-201AF LIGHT WATER (TM) It AJTFF (FREEZE PROTECTED) X veilednir.g. Svelging kan fore til: 1 Pavirkning av nvrer.e: symptomer kan vare redusert urinmengde, biod ! i ur inert og ryggsnerzer. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig irsak til tilstanden er oppkast.): sympromer kan vare hosting, pustevansker, gisping, hoste opp blod og lungebetennelse som kan vare livstruende. Informasjon om allergi, overfalsomhet: Kreftfremkallende egenskaper: I Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dette produktet inneholder en eller fiere organiske fluorkjemikalier, som muligens kan absorberes og lagres i kroppen over lengre tid, enren som utgangsmolekylet eller som nedbrytningsstoffer (metabolitter), og kan akkumuleres ved gjentatte eksponeringer. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nir de brukes som forutsatt og i henhold til veiledning. Forekomsten av organiske fluorkjemikalier i blodet til den generelle befolkningen og underpopulasjoner som arbeidere, har vart publisert siden 1970 rene. JMs epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. j j j j i 12 OPPLYSNINGER OM MILJ0FARZ. Persistens/Biologisk nedbrytbarhet: Dette produktet inneholder en eller fiere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljeet (persistente). Andre skadevirkninger, miljo: Nedbrytning: COD (Chemical Oxygen Demand) * 1 710 000 mg/kg; BOD 20 dagers (Biochemical Oxygen Demand) 494 000 mg/kg; BOD20/COD - 29%. Akvatisk criftiqhet: 99312-3 FC-201A? LIGHT WATER (TH) 1% AFFF (FREEZE PROTECTED) 96 timers LCSO for fisk (Fathead minnow (Pimephales promelas)) 310 mg/I; 49 timers EC50 for daphnia (Water flea (Daphnia magna)) - > 1000 mg/1; 96 timers ICSO for alge > 1000 mg/1; 30 minutters EC50, Microtax (Photobacterium phosphoreum) 63 mg/1; 3 timers EC50, Activated Sludge Respiration Inhibition - > 1000 mg/1. Sarg for avf allshlr.dtering iht. gjeldende forskrifter. 13 FJERNING AV KJEMIKALIEAVFALL. Metoder for fjerning av kjemikalieavfall: Spesiaiavfall. Lever avfail til en offentlig godkjent innsamler, mottaks- eiler behandlingsanlegg for spesiaiavfall. Anbefaite avfallsgrupper: HOVEDKATSGORI 07: AVFALL FRA ORGANISES PROSESSER. UNDERKATSGORI 07 06: Avf all fra PBD3 av fettstoffer, spe, rengjaringsmidler, desinfeksjonsmidler og kosmetikk. EAX-koce 07 06 03: Halogenerte organiske lasemidler, vaskevssker og moderlut. Avfailstoffnummer: 7151 Organisk avfall med halogen. Spesieiie komponenter: fluorforbindelser. 14 OPPLYSNINGSR OM TRANSPORT. ADR-kiasse: siffer: bokstav: margnummer: CEFIC-kort: UN-nummer: ICAO-klasse: IMDG-klasse: L9M93o1.r2e-j3.no.: rage z y uj j t FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) IMDG-side: EMS-nr.: MFAG: Svrige opolysninger: Ikke transporzfarlig gods. 15 OPPLYSNINGER OM LOVER OG FORSKRIFTSR. Merkeetikett: Symbol(er): KELSESXADELIG (Xn) Inneholder: 2-(2-butoksyetoksy)-ezar.ol 19 % Etyler.glykol 14 % R-setninger: Farlig ved svelging. Irriterer aynene. S-setninger: Unngi inninding av camp/spraytetike. Unngi konzakt med ayr.ene og langvarig kontakt med huden. Fir man S t o f f et i aynene; skyll straks grundig med szore mengder vann og kontakt lege. Etter hudkontakt, vask .med sipe og vann. Ved svelging, kontakt lege omgende og vis aer.r.e beholderen eller etiketten. 1 \ YL-gruppe: 00 -- Korr, yrkeshyg. l.uftbehov: 0-30 m3/l Ovrige opplysninger ora merkeetiketten: Ovennevnte merking er basert pi tidligere merkeforskrifter og vil vare i finne pi produktet en tid fremover. Merkingen vil imidlertid erstattes av folgende merking, som er basert pi reviderte merkeforskrifter av januar 1998. Symbol: IRRITERENDE (Xi) _ Inneholder: 2-(2-butoksyetoksy)-etanol, 1,2-etandiol Irriterer aynene. Ur.r.gi inninding av damp/spraytetike. Unngi kontakz med aynene. Unngi langvarig eller gjentatt kontakt med huden. Fir man Stoffet i aynene; skyll straks grundig med stre mengder vann og konzakt lege. Etter hudkontakt, vask med sipe og vann. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. 0 u9i9o3.1re2j-.3no.: rage j u oj J i FC-201AF LIGHT WATER (TM) 1% AFF (FREEZE PROTECTED) YL-gruppe: 00 Spesielle lover og bestemnelser: 1. Forskrift om utarbeideise og distribusjon av Helse-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking m.v. av stoff som kan medfare miljafare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfai!. 6. Etc. 16 ANDRE OPPLYSNINGER AV BETYDNING FOR BRUKERNES SIKKERHST OG HELSE. Anvendelsesomrder: Filmdannende skumkor.sentrt for slukking av brann i hydrokarboner. i j Viktigst kilde(r) ved urarbeidelsen av HMS-databladet: Datablad fra 3M General Offices, St. Paul, Minnesota, USA. rsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter,fastsatt i januar 1998. Etc. I ; j Egne opplysninger: * Informasjonen pi dette HMS-databladet er i henhold til vire nvrende opplysninger og beste oppfatningangende riktig bruk oghndteringen av dette produktet under normale forhold. Enhver bruk av produktet sm ikke er i samsvar med dette HMS-databladet, eller som medfcrer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. | ! I j j i i -i I i i i 002192 99312-3 i UC J A UJ J I FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) FOLGENDE PRODUKTER DEXXES AV DETTE HMS-DATA3LADET: 3M ID-nr : ZF-0002-0842-9 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 200 L 3M ID-nr : ZF-0002-0341-I 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF,1000 L 3M ID-nr : XF-0008-0205-8 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, Bulk 3M ID-nr : XF-0008-0203-3 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF,1000 L 3M ID-nr : XF-0008-0202-5 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 200 L 3M ID-nr : XF-0008-0200-9 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 20 L B0 0 2 1 9 BEST COPY AVAILABLE RF - Rogaland Research P.O.Box 2503 Ullandhaug 4004 Stavanger Norway Telephone: +47 51 87 50 00 Telefax: +47 51 87 52 00 Visiting address: Professor Olav Hanssensvei 15 Bergen: Thormohlensgt. 55 5008 Bergen Norway Telephone: +47 55 54 38 50 Telefax: +47 55 54 38 60 Internet: http://w w w .rf.no