Document Gmp4evZ675y8geb7xBNBND1bN

AR R26 ~ 0788 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (SCUD) IESTSUBSTA00N 000C 000E Identity: ArefmeirxrteudretocaosntPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m2a0y6aolrsoL-be 3243. (1-Octanesulfonic acid) (CAS # 2795-39-3), RiseFmCa-r2k0s8:, ideTnthiefi3edMbpyrtohdeucltaiboonraltootrnyuamsbe"rSawmapslenoCt".noCtuerdr.enTthienftoersmtsataimonp.le i7n8d.i9ca1t%eswaittiesr,a 1m.ix3t3u%reSuolft0o.n6e7f%oaPmFerO,S,1%17s.o5d%iudimeotchtyyllensuelgflatyec,ol0b.u0t4yl%ether, 0s.od0i5u%mbleanuzroytlrisualzfoaltee., 0.5% polyoxyethylene monooctylpheny - ether, and The following summary applies to a mixture with incompletely crehfalreaccttteorxiizceitdycoofnctehentfrlautoirooncsohefmiicmaplurciotimepso. nDeanttaomfatyhentoetstacscaumrpaltee.ly METHOD: 0000 Method: Methods for Toxicity Tests with Aquatic Organisms as formulated by the Committeefor Toxicity Tests with AquaticOrganisms TGyLpPe:: NAocute static YSepeacriceosm:plGeatmemd:ar1u9s7f4asciatus Supplier: In-house cultures maintained at Bionomics, EG&G, Inc., WAnaarleyhtiacma,l MmAon.itoring: Not given. Exposure period: 48-hours TSteasttisotricgaalnmiestmhaogde:: 12-24 hours old fourth instar TLso (median tolerance limit) values calculated using a linear regression equation. Test conditions: DDiilluuttiioonn wwaatteerr:chWeemlilswtartye:r, `aNogtegdiv2e4nhours Lighting: Not given SEtxopcoksuarned vteesssteslosl:ut2i5o0n pmrLepbaeraakteirosn:witNhot20gi0vemn.L exposure solution NNuummbbeerr ooff roerpglaicnaitsems:s p4er replicate: 5 . Number of concentrations: Water chemistry during the sftivuedypl:us a blank control Temperature range (0-48 hours): 20 1 C 001515 Nominal mglL. concentrations: Blank control, 3200, 4200, 5600, 7500, and 10,000 Element values: 48-hour 48-hour TLso = NOEL 5170 (4190 = 3200 mg/L 6370) mg/L. Element values based on nominal concentrations. RSuebmsatraknsc:eTReesmtianrgkwsafsielcdo. nTduhcetveadlouenstrheepmoirxtteudraepapslydetosctrhiabtemdixitnutrheeaTnedstnot the fiuorochemical proportion alone. conclusions 000000000000 The FC-206 48-hour TLso was confidence intervalof 4190 to determined 6370 mg/L. tTohbee458-1h7o0urmgn/oLowbistehrave9d5e%ffect level (NOEL) was 3200 mg/L. Submitter: 3M Paul, Minnesota, C5o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St. b0 ataquaur0 y 000 tReesltiianbgi.litHyo:weKvieirm,istchhersaankmipnlge=pu2r.itTyhiwsasstundoyt pmreoeptesrltyhcehcarriatcertiearfiozredquaalnidtythe study in the lacks analytical solution. confirmation of the amountoffluorochemical proportion R0 EFERENCES0000 Treeqsutewsatsofctohnedu3cMteCdombypaBniyon,oSmti. csPa,uEl,G&MGN,, Inc., of 1974. Wareham, MA at the o0 TmeR0000000000 Last changed: 6/28/00. 001516 TR Se" 3 BIOASSAY REPORT SUBMITTED T0 3M COMPANY ST. PAUL, MINNESOTA ACUTE TOXICITY OF SAMPLE C TO WATER FLEA (Daphnia magna), AND SCUD (Gammarus fasciatus). Bionomics, 4 EEnGvi&rGon,menItnac,l Consultants 790 Main Street Wareham, August, 1M9a74ssachusetts 001517 METHODS AND MATERIALS ' . These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of water flea (Daphnia magna), and scud (Gammarusfasciatus) to Sample C, a brown liquid, was evaluated under static bioassay conditions for a 48-hour period. Results were expressed as the median tolerance limit (Tig), the nominal concentration of the test compound in water causing 50 percent immobilization for the Daphnia end Gammarus. The TLy value and its 95% confidence : intervals were calculated by converting the test concentrations and the corresponding observed percent ) mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. % tL Procedures for static bioassays as described in the Methods for Toxicity Tests with Aquatic Organisms as formulated by the Committee for Toxicity Tests with Aquatic Organisms were followed, except for those conditions described below. The Daphnia, and Gammarus were acquired from laboratory cultures. The Dephnia and Gemmarus bioassays were conducted at J 20C (41.0). .The invertebrate tests were conducted in 001518 E Page two \ 250 ml beakers containing approximately 200 ml of well water which was aged for 24 hours. Five 12-24 hour old Daphnia, end five fourth instar Gammarus were placed in each beaker (4 replicates/concentration). In the Daphnia bioassays, Daphnia food! was addedat a rate of 0.5 ml/liter after the introduction of the compound into the test vessels. RESULTS The predicted TLy, values and 95% confidence intervals - for the Daphnia and Gmmarus are presented in Table 1. Table 2 presents a summary of observed immobilization ) for each individual test after 24 and 48 hours. -_-- Food was prepared by homogenizing 5 g of starter trout ; food and 1 g of cerophyl in 100 ml of water. 001519 ) . SUBMITTED BY: EBiGon&oGmi,csInc, E79n0virMoanimnenSttarleetConsultants WAuagrueshta,m, 1M97e4ssachusetts PREPARED BY: ) Robert E. Bentley CIhniveefs,tigAactuitoensToxici APPROVED BY: Bevier Hasbrouck Sleight, IIT DiFrreecsthowrat,er Programs ' 001520 Table 1 -- Acute toxicity of Sample C to Daphnia and y Gammarus." The data are based on results of bioassays conducted at the fish toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species _-- TLgo-Milligram active ingredient/liter rer ro--- No Effect l(emvge/1l) Daphnia 310,060 5.850(%,810-7,110) 3,200 Gammarus --- 5,310(4,340-6,510) 5,170(4,190-6,370) 3,200 . Bioassay conducted at 20C (+ 1.0), Daphnia 12-24 hours old ) + at the initiation of tests. PBicassays conducted at 20C (+ 1.0) Gemmarus 12-24 hours 01g. at the initiation of tests. : ) 95% confidence intervals. J 001521 5 Table 2 -- Concentrations tested and observed percent immobilization for Dephnia and Gammarus exposed | io Sample C after 24 and 48 hours. --- Species --- Concentration (me/1) immobilization Aine iastion observed sbeerved Daphnia 10,000 _ 20 100 7,500 25 50 5,600 4,200 o ko 0 10 3,200 o o 3 control 0 0 Gammarus --- 10,000 7.500 5,600 4,200 3,200 control 100 Bo 60 4s -o o 8100 75 50 _0 [4 OO 00152%