Document GmjEbV0gmG4dGqJ3No61EErwv
P M X S rO H 0 <\
MICROBIOS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-X or R1904. (Glycine, Nethyl-N-[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991-51-7)
Remarks: The test sample is FC-129 referred to by the test laboratory as R1904. Current information indicates it is a mixture of 42% test
C -Csubstance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% 4 7
fluoroalkyl carboxylate compounds. Test material was from 3M production lot 930014, mfg date 3/97.
The following summary is abbreviated because the test sample is a mixture of the test substance in a water/organic solvent solution. Data may not accurately relate toxicity of the test sample with that of the test substance.
METHOD:
Method: Microbios Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1997 Species: Vibrio fischeri formerly Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition Replicates: Two Statistical methods: EC10 and EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics Microtox Reagent.
RESULTS
ECNominal concentrations: Blank control, 125, 250, 500, 1000 mg/L
Element value: 5-minute 50 = 354 (316-396) mg/L 15-minute EC50 = 290 (261-323) mg/L 30-minute EC50 = 274 (248-302) mg/L
All element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The value reported applies to that mixture and not the test substance. No attempt was made to
000419
determine the impact of the presence of the organic solvents or what portion of the toxicity can be contributed to the Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-129 30-minute EC5ofor Vibrio fischeri was determined to be 274 mg/L with a 95% Confidence Interval of 248-302 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted at AScI Corporation of Duluth, Minnesota at the request of the 3M Company. OTHER Last changed: 5/17/00
00i0420
AScI Corporation
TEST .
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DATE
____________________________
TECHNICIAN ^ .rr-,
________________
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Effective: 6/1993
000421
pige 3 of I I
Sponsor Sponsor Study ID#: AScI Study ID#: Study Director: Test Substance: Reference Substance: Test Organism: Type of Test: Test Guideline: In-Life Phase Dates:
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1/ i Or Co
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Effective: 6/1993
000422
Page 7 of 8
MICROTOX DATA REPORT
Basic Test
FILE: 97070901.K5
R1904 dark yellow in c o l o r ;consistency of cough syrup. 2000mg/l
stock
Test Time: 5 minutes
Osmotic Adjustment:NaCl
NUMBER
I0/IT
CONC.
CR/GAMMA % EFFECT AV % <? DIFF
Control 96.99/ 78.33 0.0 0.8076 it Control 110.50/ 89.45 0.0 0.8095 it
1 116.03/ 67.32
125.0000
0.394 it
28.2
2
110.55/ 60.09
125.0000
0.488 #
32.8
30.5 Q 2.3
3
122.19/ 53.45
250.0000
0.848 #
45.9
4
108.70/ 53.42
250.0000
0.645 it
39.2
42.6 Q 3.3
5
103.95/ 35.95
500.0000
1.338 it
57.2
6
110.81/ 37.84
500.0000
1.368 it
57.8
57.5 Q 0.3
7
111.12/ 26.72
1000.0000
2.363 it
70.3
8
109.79/ 27.90
1000.0000
2.182 it
68.6
69.4 0.8
CR = CoCORRECTION FACTOR = 0.8086 tt Used for calculations
EC50 354.0 tt^lL
CONFIDENCE RANGE:316.3 TO 396.3)
CONTINUED TO NEXT PAGE.:.
TEST DATE: TIME: / r ? o
000423
>/4 7 V
FILE:97070901.K5
PAGE 2
10 ZDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD ?
3SL0PE = 0.7992
3
33
33
G3
3
A3
3
M3
3
M3
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A3
3
36
1 >z z z z z z z z z z z z z z z z z z z z z z z z z z z z z _ . __
3
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3 3
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3 _2
3
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.
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3 ______
3 _____ o
EC50
3
1010 @DDDDDDDDDDDDDDDDDDD~ DDDDDDDDDD DDDDDDDJT DDDDDDDDDDDDDDDDDDDY
100 CONCENTRATION 1000
10E4
ESTIMATING EQUATION: LOG C = 1.2247 x LOG b +2.5491 95% CONFIDENCE FACTOR: 1.11940 FOR EC50 COEFFICIENT OF DETERMINATION: R~2 = 0.97877
000424
MICROTOX DATA REPORT
Basic Test
FILE: 97070901.K15
R1904 dark yellow in c o 1or 3consistency of cough syrup. 2000mg/l
stock
Test Time; 15 minutes
Osmotic Adjustment:NaC1
NUMBER
10/IT
CONG
CR/GAMMA 7. EFFECT AV 7. q DIFF.
Control 96.99/ 72.50 0 .0 0.7475 # Con t r o 1 . 1 1 0 .5 0 / 81.76 0 *0 0.7399 #
1 116.03/ 5 8 . 65 125. 0000
110.55/ 5 2 . 30
125. 0000
122.19/ 4 5 . 05
250. 0000'
4 108 ? O / 44.87 250. 0000
i:r
103.95/ 3 0 . 48
500 0000
110.81/ 500 0000C-T
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111.12/ ',2^ -So
1000. 0000
109. 79/ 2 3 . 43
1000 0000
CR L- OCUKRh . I S H O N FAC TOR = 0.743 St : ised to" calculations
0.471 0.572 1.017 0.802 1.536 1.530 2.696 2 ,,485
# # # # # # # #
32.0 36.4 50.4 44.5 60.6 60 . 5 72.9 7 1 .2S
34.2 '-7 2r.*lB-j"il 47.5 0 3.0 C>'J n".} O 0 .0 72.1 G 0 .8
EG 50 290.1 H z C
CONFIDENCE R A N G E :261.0 TO
Sionature
X. . -
;D N ilN LjE D T 0 NEXT PAGE . ,
TEST DATE: ~^f/^ T I M E : . ,, / W -----
000425
H i tec
FILE s97070901.K 15
PAGE 2
10 ZBBBBBBBBBBBBBBDDDDDBBBBBBBBBDDDDBBBUBBDDBBBDBDDBBBBDBBDDBBB
3SL0PE = 0.7716 3
3
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_ _ __________
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3
A3 M3
3 73
M3
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10
100 CGNCENTRATION 1000
10E4
E S T IM A T IN G E Q U A o O LOG C = 1 . 2 7 3 3 k LOG b + 2 . 4 6 2 6 957. CONFIDENCE FACTOR; 1.11152 FOR EC50
C O E F F I C I E N T OF D E T E R M I N A T I O N ; R'""2 ^ 0 . . 9 8 2 5 1
000426
^ K rd
MICROTOX DATA REPORT
F ILE : 97070901.K30
Basic Test
R1904 dark yellow in color;consistency of cough syrup. 2000mg/l
stock
Test Time: 30 minutes
Osmotic Adjustment:NaCl
NUMBER
10/IT
Control 96.99/ 67.40 Control. 110.50/ 76,81
CONC.
0.0 0u 0
CR/GAMMA
0.6949 # 0.6951 #
7. EFFECT AV % <7 DI FF
1 116.03/ 54,30
125.0000 0 ,,485 #
32.7
110.55/ 48.04
125.0000
0,599 #
35. 1 O 2,4
122.19/ 42.35
230,0000
1,005 #
50, J.
4 108,70/ 41.06 250.0000 0. El40
45 =7
47,9 a j
'iu.Jr 103,95/ 27,41
500.0000
1*.636 #
62.1
i10,31/ 29,19 500 0000
.1. , 63:8 #
62.1
62,1 0 0,0
. ....
111,12/ iV =o4
1 000.0000
2,993 IT
75.0
8
109,79/ 20,62
1U U O ,0000
2,701 #
/3 0
74.0 1,0
UK L-OL'UkktUT IUN PAL- FUR # U s e d f ar c a 1 c u l a t i o n s
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EC5 0 273,6 X (?5 2 C O N E I DENUE E AN UE s248 -
" 131ft
TO 3 0 1 . 5 )
r
CONTINUED TO NEXT PAGE...
TEST D ATE:.
TIME: /T?Q___
000427
f
F ILE s97070901.K30
PAGE 2
1 0 ZDDDDDDDDDDDDDDDDDDDDDUDDDDDDDDDDDDDBDDDDDDDDDDDUUDDDDD?
JSLOF'E 0.8029
3
33
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1.0
100 CONCENTRATION 1000
10E4
ESTIMATING EQUATIONS LQG G = 1=227? x LOG b +2.4370 95*/. CONFIDENCE FACTOR; 1.10200 FOR EC50 COEFFICIENT OF DETERMINATION; R-"-2 = OT98583
'/
000428
Environmental LAabttoacrhamtoernyt A
LR/Study Number:.J R 1 3 0 W
Microtox Test Solution Preparation
Date/Time: Analyst:
- ^ j3 j3 ^ J 5 3 0
_4-iA K ,T7>
SUBSTANCE: I ____________________
1. Preparation of Stock Solution or 100% Solution
(If testing a 100% solution, record final concentration as 100% and skip to section 2)
Mass/Volume or volume/volume o f Test Substance: Diluent Used:
yod. m^ / ^ ^ ^ _l^cLLli^XulL^-t^QktL^
Final Concentration o f Stock Solution (mg/L or % v/v):
S tO C Q n n c y ff
Balance Used: iD E fU e / ATn lfX )
1
2. pH Adjustment of Stock Solution or 100% Solution
Initial pH :
pH Adjusted to: pH Adjusted with:
J d C l __
3. Osmotic Adjustment of Stock Solution or 100% Solution
Osmotic Adjuster: Comments:
^-iCQ) mg added to
CJo Il y ic Lf . C / , L | 6 m ls
Q O mL solution
_4_. __A__p_p_e_a__r_a_n_c_e__o_f__p_H___a_n_d__O__s_m__o_t_ic_a_l_l_y__A_d__ju_sl tfeAd S^tock Solution
Color: I , Cj hi' Lj Li IQUO Com m ents: l2 (SCM__ C W iS
OPAQUE
(Circle one)
L jlT K o u j C ColCf , C r T ii^ tf n f a j
c
5C. oCiQomlnments
,_______
________ i& . n c y r n i___d ^ L .,^
E ^ p . 1 /9 9-
EEF-M-3.0
0 0 0 4 2 9 Page 21 of 22
AScI Corporation/AScI-Duluth Environmental Testing Division
MICROTOX PHENOL REFERENCE TOXICITY CONTROL CHART
Test Date July 1997 July 1997 July 1997
5-M(nitng/ELC) 50 23.59 18.26 18.23
Running Mean (xng/L)
--
20.93 20.03
Standard Deviation
-- 3.769 3.086
:r:LCJimppitr
-
28.47 26.20
Limit
--
12.99 13.86
CV (%)
--
18.0 15.4
000430
AScI Corpo ration/AScI-Duluth Environmental Testing Division
MICROTOX ZINC REFERENCE TOXICITY CONTROL CHART
Test Date June 1997 June 1997 July 1997
iS-Min EC50 Running (mg/L) Mean (mg/L) 3.898 -4.481 4.190 4.125 4.168
Standard Deviation
0.412 0.294
Upper Limit
5.014 4.756
LLoimweitr
-
3.366 3.580
CV (%)
--
9.8 7.1
000431
