Document GmekM39xyv4Vzr4Dn6OO7BQ44

Younger Laboratories QiockeitU&ti . . . . AttalqMA' 5620 Telegraph Road St. Louis 29, Missouri PHONE: TILDEN 6-2540 Q&itiiiccde AnaufAM Q4^Km(V\ Jure 2f>th, 1?62 SUBJECT - Toxicological Investigation Oft Aroclar 1262 Monsanto Saaple Number 1(0 Monsanto Project Number T-62-38 STUDT CONDUCTED FCR - Monsanto Chemical Company, St. Louis, Missouri EXPERIMENTAL PR CCEDURE * A) Oral ID50 (Rats) The diluted compound was to Sprague-Dswley strain albino rats. After the app female rats we: intervals at ing data for of the method determined, groups of male and ia at increments of 0.1 fractional log iket the toxicity range thereby supplywhich, was docs according to a modification Observations succumbed were toxic symptoms and the viscera of the animals that macroscopically. The data, together with the dilution at which the compound was fed, are show in Table I. B) Skin Absorption HLD (Rabbits) The diluted compound was applied in increasing doses at increments of 0.2 fractional log intervals to the closely clipped, Intact skin of New Zealand white female rabbits. . The treated areas were covered with plastic strips and the animals placed in wooden stocks for periods up to twenty-four hours, after which time they were assigned to individual cages. Cfoserrations were made for toxic symptoms and the viscera of the animals that succumbed were examined macroscopically> The data, together with the dilution at which the compound was applied, are shown in Table II. L Thu DSW 274853 report is not to be used in any form of advertising without written permission STLCOPCB4054384 Tot Monsanto Chemical <pany St. Louis, Missouri lounger Laboratories Certificate of Analysis - Page 2 (6/25/62) - Y-62-38 SUMMARY Aroclor 1262 A) Oral LD50 (Rats) . The Oral LD60 for male and female rats was placed at 11,300 milligrams per kilogram with lower and upper limits of to 12,880 milligrams per kilogram. The compound was classed as practically non-toxic by oral ingestion in male and female rats. B) Skin Absorption MID (Rabbits) The Minimum Lethal Dose by skin absorption in female rabbits was found to be greater than 1260 milligrams per kilogram and less than 3160 milligrams per kilogram. There was one death and one survival at 2000 milligrams per kilogram. The compound was classed as mildly toxic by skin absorption in female rabbits. XOUHQER LABORATORIES The material ia this report is to be used in development of the product and may be given to responsible sales contact#, but it is not to be used by them in advertising copy. The source of this material is not to be divulged until it appears in formal publications. No exceptions tothe established rule may be made without the approval of the Medical Depart ment in St. Louis. Customers' inquiries regarding matters of toxicity are to be referred ssbefore to the Medical Depart ment in St. Louis for reply' -- Monsanto Chemical Company OS* 211851 STLCOPCB4054385 Toi Monsanto Chemical japany St. Louis , Missouri lounger Laboratories Certificate of Analysis - Page 3 (6/25/62) - T-62-38 TABLE I THE ORAL LDB0 CF 'Aroclor 1262* FOR RATS Sample led As A 50.GC< Solution 2h Cam Oil Animal No. - Sex Weight Qm. Dose Mg. / Kg. 1- Female 2- Female 3- Female hr Male 5- Male 6- Male 7- Female 8- Female 9- Female 10- Male 11- Male 12- Male 13- Female 1U- Female 15- Female 16- Male 17- Male 18- Male 19" Fanale 20- Male 230 7,9U0 215 7,9U0 220 7,9kO 235 7,9kO 250 7,9kO 230 10,000 215 10,000 225 10,000 220 10,000 230 10,000 21*5 12,600 225 12,600 210 12,600 220 12,600 225 12,600 235 15,800 2U5 15,800 2ltf) 15,800 215 15,800 230 15,800 Fate Survived Survived Survived Survived Survived Died Survived Died Survived Survived Died Died Survived Died Survived Died Died Died Died Died DISCUSSION " The Oral LDS0 for male and female rats was placed at 21,300 milligrams per kilogran with lower and upper limits of 99^0 to 12,880 milligrams per kilogram. Tie compound was classed as practically nan-toxic by oral ingestion in male and female rats. Survival time was one to six days with most deaths occurring in three days. Toxic sympfcaas included severe diarrhea, loss of appetite, lethargy, and increasing weakness. At autopsy there was infDamnation of the gastric mucosa, tissue edema, and liver congestion macroscopically. qS\N 274855 STLCOPCB4054386 Tot Monsanto Chewteal jspany St, Louis, Missouri Younger Laboratories Certificate of Analysis - Page U (6/25/62) - T-62-38 TABLE II THE MINIMUM LETHAL DC6E OP Aroclor 1262' BT SKIN ABSORPTION IN RABBITS Sample Applied As A 50.C# Solution In Com Oil Animal No, - Sex Weight Kg . Dose Kg. /Kg. Weight Change 5 Days Later Kg, 1 - Female 2,2 79k 2 - Faaale 2,7 1260 3 - Female 2.5 2000 h - Female 2,6 2000 5 ~ Female 2,k 3160 6 - Female 2.7 3010 - 0.1 - 0.2 - 0.3 - 0.2 - 0*5 - 0.3 Fate Survived Survived Died -- 7 Days Survived Died -- 8 Days Died -- 5 Days DISCUSSION - The J&nimom Lethal Dose by Skin Absorption in female rabbits was found to be greater than 1260 milligrams per kLlogrws and less than 3160 milligrans per kilogram. There was cm death and one survival at 2000 milligraas per kilogram. The compound was classed es mildly tcadlc by tide absorption in female rabbits. Survival time was five to eight days. Toxic symptom included poor appetite with resultant loss of weighty nervousness, increasing weakness* and sudden collapse. At autopsy them was pulmonary hyperemia* liver discoloration* and renal congestion. DSW 274856 STLCOPCB4054387