Document Gmaw3ZvZqdpMZELeKKV6G4LDv
WARF INSITI'U"IEI,NC.
MADISON,
WISCONSIN
are'submittedto clientson a confidentialbasis. No referenceto the work, the resultsor to the Institutien any form Roefpaodr'vsertisingn,ews releaseor other publicannouncement may be made withoutwrittenauthorizatiofnrom the Institute.
REPORT
Sample: T-1166
WARF No. 5011023 Date Received: 1-13-75
Submitted by:
J. E. Long 3M Company St. Paul, Minnesota
Testing requested:
Skin Irritation Eye Irritation
Conclusion:
Under the conditions specified the pro-"'*uchtas a primary skin irritation score of zero and eye i@rritationscores of 23.67, 23.67, 21.67, 13.50 and 3.33 at 1, 24, 48, 72 hours and seven days respectively for unwashed eyes.
The product is not irritating to the skin and is irritating to the eye.
Report approved by:
Signed l@/and for WARF Institute, Inc. Date: January 28, 1975
Donald L. Hal7ris,-"b.V.MJ'J Chief Pathologist/Toxicologist
FORM 36 -4
AlFwARF
MADISON,
INSTITU-1 INC.
WISCONSIN
Sample:
T-1166
WARF No. 5011023
Primary Skin Irritation:
Method: Albino rabbits were used for this work. They
were housed in individual screen bottom cages and fed water
and rabbit chow ad libitum. The hair was clipped from the
back and flanks
the animal. Five tenths of one milliliter
of test material was placed in intimate contact with the
skin on prepared test sites (abraded and intact), and covered
with gauze patch and tape to maintain contact with the skin.
After a 24 hour contact period, the coverings were removed
and the degree of erythema and edema was recorded according
to the scale below. Readings were taken again at 72 hours
and the 24 and 72 hour readings used to determine the primary
skin irritation score.
Results:
Animal Number
1 2 3 4 5 6
24 Hours Abraded Unabraded
72 Hours Abraded Unabraded
Average
no noted irritation all sites read negative
Primary skin irritation score: 0
Scale for Evaluation of Skin Reaction
Erythema and Eschar Formation Score Edema Formation
Score
Slight erythema Defined erythema Moderate to severe Severe erythema to
eschar formation
erythema slight
1
Slight edema (barely
2
perceptible)
1
3
Defined edema (edges
definite rising)
2
4
Moderate edema (area
raised 1 mm)
3
Severe edema (raised
more than 1 mm)
4
Score equals sum of erythema and edema readings. Skin irritation index equals average of 24 and 72 hour scores.
r-C)RW,28-4
IN WARF INsriTuTEI,NC.
MADISON,
WISCONSIN
Sample: T-1166 Eye Irritation:
WARF No. 5011023
Method: Adult albino rabbits of the New Zealand White variety were placed in a collar such that the animals could
not rub their eyes. One-tenth of a milliliter (0.1 gm for solids) of test substance was instilled in one eye, the other untreated eye served as a control. A series of six albino rabbits was used for each substance. The reaction to the test material was read according to the scale of scoring for damage to the cornea, iris and the bulbar and palpebral conjunctivae at 1, 24, 48, 72 hours and seven days after eye instillation. Any residue of the test material and accumulated discharge are flushed from the eye each time they are scored.
Administration:
Concentration of test sample: as submitted
Diluent or solvent: none
Special washing: none
Results:
Rabbit Number
1
2
1
3
hour
4
5
6
1
2
24
3
hours 4
5 6
1
2
48
3
hours 4
5
6
Cornea Opacity Area
Iris
Conjunctivae @-kedness Chemosis Discharge
1
4
0
2
2
2
1
2
0
2
2
1
1
2
0
2
2
2
1
2
0
2
3
2
0
0
0
2
2
2
2
2
0
2
2
2
1 hour eye irritation score: 23.67
1
4
0
2
2
2
1
2
0
2
2
2
1
2
0
2
2
2
1
2
0
2
2
2
0
0
0
2
2
2
2
2
0
2
2
2
24 hour eye irritation score: 23.67
1
4
0
2
1
2
0
2
1
2
0
2
1
2
2
2
0
2
1
1
0
0
0
2
1
1
2
2
0
2
1
1
48 hour eye irritation score: 21.67
FORM 28-4
]IN WARF INSTITUTE,INC.
MADISON,
WISCONSIN
Sample:
T-1166
Eye Irritation: (continued)
WARF No. 5011023
Results:
Rabbit Number
1 2
72
3
hours 4
5
6
1
2
7
3
days
4
5
6
Cornea Opacity Area
Iris
Conjunctivae Redness Chemosis Discharge
1
2
0
2
0
0
1
2
0
2
0
0
1
1
0
2
0
1
1
2
0
2
0
0
0
0
0
2
0
0
2
2
0
2
0
0
72 hour eye irritation score: 13.50
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
1
2
0
0
0
0
0
0
0
0
0
0
0
0
1
2
0
0
0
0
7 day eye irritation score: 3.33
FORM 28-4