Document GmaEN0q79GqLeVrRKj5dLerkm

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-602. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M producton lotnumber was 501. The testsample isFC602-X. Currentinformationindicatesitisa mixtureof 1% PFOS, 12% diethyleneglycolbutylether,61.05% water,20% ethyleneglycol,2% Sultone foamer,1% sodium octylsulfate,1% sodium decylsulfate,I% magnesium sulfate0,.7% xanthangum, 0.1% starch,0.1% n-(3-chloroallyl)hexaminium chloridea,nd 0.05% tolytriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reftct the toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Method 202, U.S. EPA Method EG-1. Type: Acute static GLP: No Year completed: 1988 Species: Daphnia magna Supplier:In-housecultures.Originalloybtainedfrom U.S.EPA ERL, Duluth, MN. Analytical monitoring: Temperature, pH, and dissolvedoxygen. Exposure period: 48-hours Test organism age: <24-hours old Statisticalmethod: LC50 values calculatedusing a computer software program. Test conditions: Dilutionwater: Carbon-filterewdellwater Dilutionwater chemistry: Hardness: 240 mg/L as CaC03 Alkalinity: 220 mg/L as CaC03 Conductivity: 500 pmhos/cm Lighting: Not given Stock and testsolution preparation: A primarystocksolutionof 1000 mg testsubstance added to 1L wellwater. Exposure concentrationsprepared by additionofstocksolubontodilutiownater inexposure vessels.InitiaplH of the stocksolutonwas 8.2. Exposure vessels: 250 mL PyrexD glassbeakers with200 mL exposure solutionand a water depth of6 cm. Number ofreplicate2s: Number ofdaphnids per replicate1:0 Number of concentrations:fiveplusa blankcontrol Water chemistryduringthe study: pH range (0-48hours): 8.1- 8.2(controelxposure) 8.1- 8.3(1000 mg/L exposure) Temperature range (0-48hours): 20-22 OC Dissolved oxygen range (0-48hours): 8.7- 9.2mg/L (controelxposure) 7.5 - 9.2mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Bk control1,00, 180, 320,560, and 1000 mg/L. Elementvalues: 48-hourEC50=1000mg/L Element values based on nominal concentrations Remarks: Testing was conducted on a mixtureas described inthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-602 48-hour EC50 was determined to be 1000 nV/L, the highest concentrationtested. Mortalityobserved was 50%. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking = 2.This study meets the criterifaorquality testing.However, the study lacksinformationon purityof the testsubstance and itlacksanalyticaclonfirmationof the amount of fluorochemicalproportion insolution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St.Paul,MN, Lab Request number Fl 151, 1988. OTHER Last changed: 6/26/00. EWIRONMEWAL LABORATORY IDAPMIA TOXICITY DATA &M TEST TYPfi.U'.Q-nIAZrL-Ag <@4L%-4@r-aiEMICAL TESM PC- bc3a- X Li 1,.ql- 2LAn-,Ro u4@r Aic- TEST ORGANI.%Il)ap@nkck Magana SOURCE U@SL-PA EA2-L ]),-,iLA, MAI -DATE RECII)2-lq-gb AGE OF TEST ORCANINS:&24 hrg- old instar (neonates).,NOO.RGANICM PER 1WA7mw-LL- TEST VESSEL: 250 ml pyrex glass beaker TEST SOLUTION VOLUME: 200 mi WATER DEmi: 6 rm TDE SrARTM_L*eitn p VY-N -- DATE STARTED-,3-zL.Y---i-?k-DATE C(I\IPLETED 3-/7-.q55) DILJJTION TER Carbon-filte=d well water (Temp. a 0 OC D.0 -3, ;2,- M" lDH AMLYST, !LT Ecso(95% Confidence Linits) lhtin ma )L Test Initial Total Vessel Conc. 0un No. mg/L AddgA m Cont rol 0 )80 33 .-1-3 3,20 I-q s@o 24 Hours Total No. pH Dead D.O. mg/L 48 Hours otal No, pH Dead D.O. MS/L C) 0 7,@RCo? 7,61 Comments: Test Temp. OC Initials& Date S-TCC-V Solo-I t6h Ib'in n ma L Uje t@54CL,) I I-A I Test methodologyconformswiththe followingtwo testprotocols: (1) OECD #20Z-"Daphnia sp., Acute Imobilization Test and Reproduction Test". (2) US-EPA #BG-1 "Daphnid Acute Toxicity Test". ENVIRD.I-IENTAL LABORATORY IDAPHNIA TOXICITY DATA SHEET LR F//"S-i TEST TYPEq@g-,UP- AcLi+e-,:@4n-tic-OM4ICALTESM TEST ORGANIS Eust-PA-E-YzL-ht-,it-.+k, M.v DATE RE ID-&jl--" AGE Or- TEST ORGANINS:!<24 hrs. old instars (neonates)N.O. OW@MISMS PER IREATHW--LD TEST VESSEL: 2SO ml pyrexg glassbeaker TEST SOLLTRIQNVOLUME: 200 mi WATER DEM: 6 rm TIME STARTED-J.'t)r1-2ix DATE srARTED-azi-@@ DATE c(zipLETE-i3) 7 -c9s.) DjUrrIONlfkTERCarbon-fi]tered well water (Temp.-aa-2C, D.O._9,@k ma 9LL pli ANALYST_4:!--a@ &Ax-li fFf.Cs(o951ConfidenceLimits)->-4-@ 1/1t%,(I - ti ih litro Test Vesse No. Initial Cmc. mg/L Totaal l 0 Added 24 Hours- 48 Hours Total No. pH D.O. Total No, pli D.O. Dead gL mg/L Dead mg/L Control 0 /9c, @@go 5L^ & 12-57 16,CL) I A 06 f7, 9,37,67 Coments: Test Temp. OC Initials & Date 3- eq C991 <AA 7 0 CC luilt-%tn6 /nt)r-, /I-u--elWlcA.4er /9-" lox/ QaLren-@l:cgnml2l~ig MI,4,C@Elp E4-,t+k,- tcell (.k-,CAer h 14idL-11-2If::&I Test methodologyconformswith the followingtwo test protocols: (1)OEM #202 "Daphniasp., Acute ImobilizationTest and ReproductionTest". (2) US-EPA NEG-1"DaphnidAcute Toxicity Test". 03/24/1988 ENVIRONMENTAL LABORATORY FINAL REPORT 1 LAB REQUEST NO. F1286 REQUESTOR NAME: RR ROBIDEAU/SAB DEPARTMENT: 0222 PROJECT NO: 06 DATE RECEIVED: 03/02/1988 DESC: CARBON-FILTERED WELL WATER - FEB. 1988 CONTRACT LAB: PACE CONTRACT LAB COST: 144 3M E-L HOURS: 1 EXP COMP DATE: 03/23/1988 DATE COMPLETED: 03/23/1988 ORIGINAL SOURCE OF, WELL WATER, 3M WELL #2, ST. PAUL, MN. -------------------------------------------------------------------------------- SAMPLE DATE CODE DESCRIPTION RESULT MIN DET LIMIT -------------------------------------------------------------------------------- 1 03/02/88 ROOM 4 WELL WATER *COD <5 MG/L 5 *SPECIFIC CONDUCTANCE 500 UMHOS/CM *AMMONIA NITROGEN - as N *p 0. 1 *SOLUBLE FLUORIDE (PROBE) *STANDARD PLATE COUNT *TOTAL DISSOLVED SOLIDS 17 COL/ML 280 MG/L *TOTAL ALKALINITY (PH 4.5) 220 MG/L *TOTAL *TOTAL ----'TOTAL *TOTAL *TOTAL COLIFORM HARDNESS RESIDUAL SOLIDS SUSPENDED CHLORINE SOLIDS <1 COL/lOdML 240 MG/L <0.02 MG/L 280 MG/L <1 MG/L * = CONTRACT LAB SUBMITTED BY: DATED: