Document GmJDJy6mxXGQ0dXJkm45bnDzx

AR 226.- 0792 SEXUTMIMNAGURIYSHFIONRGWAOGREKNTPSEMRAFNOURFMAECDTBUYRTEHDEBGYEMRUMLATINPALREMCYOMOPNANFIOEASM TEST SUBSTANCE Identity: AbemriexfteurrreedcotnotaaisniPnFgOSpe,rfFlCu-o9r5o,ocotraanessaufconaotem, pwhioochfnmFeCa-yn20atl6s.o (1-Octanesulfonic acid) (CAS # 2795-39-3). aRemmiaxtrukrse:ofT0h.e6t7e%stPsFaOmSp,le17is.F5C%-d2i0e6t.hyCluerneregnltycionlfobrutmyaltieotnheirn,di7c8a.t9es1%it is wlaautreyrl,su1l.f3at3e%, S0u.l5t%onpeolfyooaxmyeert,hy1l%enseodmoinuomooccttyyllpshuelnfaytle,et0h.er0,4a%nsdod0i.u0m5% tolyltriazole. foTrhepfuorlploosweinsgofscuommmpaarryianpgpltiheesetnoviarsotnumdeyntdaolnperbopyetrhteieGseorfmfaonamArmy e20x6t.inDguaitsahminagyangoetntasccfurroamtemluyltriefplleectcotmhpeaenniveisr,onimnecnltuadilnpgro3pMe'rstiFeCs-of the fluorochemical component in the test sample. STUDIES SP (1M0,ic2r0o,toaxnTMd),3072m-ihnouutreEECCssooffoorrgPrhoowttohbainchtiebritiiuomn opfhoFsrepshhorWeatuemr Algae L(SCcsetnoedzeebsrmausbasrubblsipnigc(atBursa)c,hy2d4a-nhioourreEriCos),, CfohremDiacpahlniOaxymgagennaD,e4m8a-nhodurand 5-day Biochemical Oxygen Demand METHODS: Microbics Microtox "BASIC" Procedure Algae growth inhibition per EPA guidelines dated 05-06-1982 Daphnia toxicity according to DIN 38412, Part 11 Zebra barbling toxicity according to DIN 38412, Part 15 RESULTS Report Date: 3/29/90 i`fTthheisECiszofofrotrhPeh1o0t,o2b0a,ctOerr3i0umpmihnoustpeoerxepousmuwrae.s 15,000 ppm. tis not dear 0601533 The 72-hour ECso Growth Inhibition for Scenedesmus subspicatus was 160 ppm. `The 24-hour ECs for Daphnia magna was 2000 ppm. The 48-hour LCs for Zebra barbling (Brachydanio rerio) was 4086 ppm. The Chemical Oxygen Demand (COD) was 24,600 mg Oz /L The 5-Day Biochemical Oxygen Demand (BODS) was 8700 mg Oz /L DATA QUALITY Reliability: Kiimisch ranking = 4. All study values come from a summary list only. No raw data or method documentation was available. -- TH --c -- m-------------- Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 6/26/00 001534 240 Germen Army Investigations of toxic effects and biological degradability of foam extinguishing agents in the waste water byExkehard Ising : | Investiofgtaoxticeiffoecntss. .In thewastewater Pago2 1. Introduction OnApril ofthe 15, 1985, theFederal MinfoirInsterntalAeffarirs Introduced a revised andexpanded version Index of Water Endangering Materials ) f`oTuhrewaitnedrexelnidtsahtnesgemroisntgimcpaotregtoarnitecshe(mWiEcCa)lcacocmoproduinngdtsoavthaeliarbwlaotoenr etnhedamnagrekreitnagndpodtievnitdieasltheminto. WWEECC32 veharmymthualrmmfautlermiaatlesrials WEC mildly harmful materials _ WECO general harmless materials All chemical materials not yet fisted in the catalogue, are considered water endangering unti the authorized `Subcommit for"Storageand Transportation of WaterEndangeringMeteias"a theFederalMinistyforInternalAffairs has decided about their classification. In casa of midures of materials, the incorporation ino a water endangering category is decided acctotohedratamdeasiurednongthemixture sat. fish and mammals. Atthefocusoftheevaluationof amaterial regaitsrenddanigenringg potfeortnhetori ganaismlsliving inwater orusing it -e.g.as afood -is ts acute toxicity, especfoirbaacltelriya, algae,smallcrayfish, Tleegsitssloatnuraeqfuoartwiactoerraganndiswmasst(ebiwoaatsesraeyvsa)luaarteioinn.cludedamongtheproceduresprescribedbythe tis generally accepted: 1. Principally,bioassays cannotbe replacedby thechemicalanalysis. 2 Bioassaysontheonehandandchemicallphysicalprocedursson the other, complement `ach ther (see among others: German Unified ProceduresforWater, Waste Water and Sludge Examination, DIN 38412). GHB eaeA.30No.1(1969,pages173-252 001536 Investiogfatotxiic ons efects. ..in thewastowater Pages Dutrheeivalnuatigon of the effecotfs ~ chemical parameters, 9. toxic materials containedinwaterand physical parameters, 0.9. supplyof energy,waterextraction theattantionshouldbepaidtotheuseofprescribedtestorganismsandto theconformanceto standardtestconditions. Thereproducibilityandstatisticalvalidityofthe asusshouldbesecured. + * "Thefollowingtypesof testorganismsshouldbeusedinstandardizedbioassaysin waterandwaste water: ~bacteria as chemoheterotrophs _ alga asprimaryproducers -protozoans assingleprimaryandsecondaryconsumers small crayasfmicseh! consumers fish as muticsl consumers with acentral Nervoussystem. Atthesametime, theyarethefinal members intheaquatic food chaanidconllesct persistent chemicalsin ther tissues. `Addiionaly, certain saprobicsystemscan beused in biotestsasmodelsofcompleteecosystems. Particularly microcosmsand activated sludgesystemsconsofivasriotusbiactneriga,fungi, protozoansand worms. 2. Problemdefinition AccordingtothedsfinionsinwatersupplylawandwastewaterhandlinglawIncludingpertinent Implementationprocedures,theFederaldefensaforcesaofrequertly quotedasthecauseofhamul materialsdischarges towatersandcommunalwastewatertreatmentplants. Pariularlyduring, e.g. - useofextinguishingfoams duringtrainingsandactions -ransportatainodn storofafgoaem agent concentrates considerableham canbe donetosurfaceandground waters. 001537 Investigations of toxic effects... ...In the wastewater Page + Toevaluatethe dangerpotentialofthefoamconcentrates notyetclassifiedinthewaterendangering catagories, their examination and miltary-intomal temporary classification into water endangering classes is necessary. The Federal Office forDefense Technology and Acquisitiion Koblenz, has `authorizedthedefensascientifcService Officeofthe FederalAmytoconductthenecessaryresearch and the subsequenttemporary Army-intemal WEG classification. Th research, leading to establishing water endangering categories of foam extinguishing agent concentrates, producesatthesame timevaluesofacuteorganismtoxicitiesofthe,mostly3%, appication solutions. 3. Feoax m tingageuntsians d thh eirbiioanssagys 3.1 Totalof 16foamextinguishingagentsrepresenting differentkindsoffoamagentswerecompara- tively evaluated in bioassays (see 3.2) regarding acute toxicityoftheirapplicationsolutions to water `organisms rates. and subdivided into waterendangering categories from the point of viewoftheirconcen- In particular: -5 multirange foam agents -6fim-formingfoam agents -13pfriomtefionrmfionagmfaugoernitnse.-protein foam agent -1special foamagentfordeep-rypanfires `The formulationsofthefoamextinguishingagentsareproprietary Information andunavailabletothe public. Afterconsultationswithmanufacturers, thefollowingmaterialscanbelistedastoxiccompo- nents: - organic surfactants prgelysceorlvsat(ei.vge.sethylengycol, butydiglyco) organic solvents organic acids - inorganic satts ten 001538 Investigationsoftoxicefects .. ...inthewastewater Pages Presentedtestresultsarenewandnotyetrevealedtothemanufacturercompanies. Therefore, productnamescannotbepublishedhere. Hence,theresuttableswillhavetheproductnames substituted by class descriptions including running Rem number. | | 32 Listingofthebioassayused(shortdescription ofeach testwiththefolowingresu table - see4.5 through 4.5). . 32.1 Bioluminescence inhibitiontestwithPhiotobacteriaPhosphoreum In microtox analyzer, model 2055. (Beckman Co) 3.2.2Algae growthinhibitiontestaccording tothetestguidelinesoffederalEPA dated05-06-1962. 3.2.3Daphniashorttestaccording to DIN38412, Part 11, 3.24 Fishtest according to DIN 38412, Part 15. 3.2.5 Determinatofitohnebiochemicaloxygendemandbytheapplicationsolutionsand theidilutionswith water during consecutive days (BOD 5). Apparatus: Sapromat B12, Voth Co. Incomparisonto BOD 5,themeasureimncelundetd: `chemicaloxygendemandoftheapplicationsolutionsandtheidion (COD). Apparatus: Or. Lange digital photometer LP 1W. 4. Resutts -- `Theresiswillbedescribed inthefollowing (chapter4.1.t04.7)intheformoftableswithassociated text. 001539 Investiofgtoaxitceifeoctns.s. ...inthewastewater Pages 41 The bioluminescence ishibftion test with Photobacteria Phosphoreu in microtox analyzer, model 2055by BeckmaCon. 4.1.1 Short descriopftthietoesnt Thetestorganism PhotobacteriaPhosphoreuris a marinebacteria.Thiskind ofbactiesprresienat inimmensequantities inthesealayersclosetothesurfaceandcausesthesocalledmarine `phosphorescence.Theunderlyingchemicalprocessisalucterine-ucipreorcaessse. Damagetso thePhotobacteria, 6.9.duetointoxications,resultintheinhibitionofthe lucierine-lucieraseactivty. `The endeffectisthereductoiftohnelightemission. "This, n efverysimple,principleisused inthemicrotoxanalyzer: Alightransparent glass cellisplacedinalightightmeasuremheenadt.Theglasscell contain1s ml of asuspwiethnPhostobiactoerina.ThelightemittedbythePhotobacterafallson aphotoreceptor, isconverted into elect energy, amplified, dispalndarecyorededd. `Thelightquantity emittedby aspecimenof untreatedbacteriaissetinthe displaytothevalue of 90%. "This quantityistantamoutnot: "natural, uninhibited fight emission". Speciofmeqeuanlquanti ofbacteriaandequal bacteria qualty withtoxic substancesadded respondwith areductionofthelightemission.Accordingly,thedisplayvalueliesbetween90%and 0%.The0%-valusindicates thatthephotobacteriaofthespecimentreatedinthiswayisnotcapable ofight producantyimooren. Fig. 1: Measurement mangemenotfmicrotoxanalyzer 001540 Page 6a Microtox- Analyzer: Measurement configuration E [] | -'A pps contin EN Tt Display 3000 1 Tempering gg:Z `Amplifier -- = Investigaotfitoonxisc effects... ...inthewastewater Page 4.1.2 TheresultsofthebioluminhiibitnionteesstwicthPehotnobaccteeriaPhosparheasosermbleeduin Tabl1e. Inthefirstresultcolumnarstheeffectiveconcentrationsofthe 16testedfoamagentsin U1ofwater lead1oi.an50g% inhibitionofthe ight emissionbythe light bacteria (= EC50). Inthesecondresultcolumn arelikewiseeffectiveconcentrationsleadingto a 10%inhibitionofthelight emission (=EC10) andrepresenting the so called taxichy thresholdsofthefoamagents tothe Photobacteria. `Thenegativedecimallogarithmsofindividual EG10 resultaftermutiplicationbythefactorof-1nso called evaluationandendangering figuresoftheIndividualfoamextinguishingagentstoPhotobacteria Phosphoreum(seeresultcolu4mannd s5). According to the evaluation scheme of the "Comission for Evaluation of Water Endangering Materials", the determined evaluation and endangering figures (EE figures) indicate the following toxihycategories: EEfiguresof 60andhigher EEfigures 401059 EEfigures 201039 EEfiguresunder 20 Highly toxic totest organisms +Ttootesxtorgianicsms +Lowtoxtoitesctorigantisyms + Generally nontottesotorxgainiscms 00154z HE Dat emsoE e Tonictyof fp oametxtoRingoEuinsEhisngalgenotC sre S came Page7a ---- | | bamSeoo vo | mE=oEm,||| mS SEemee aomtear wtazt || 02s | ooso isiorig i" oooer || amnm ww | am 0"0 wo fgr ta tos | 2s .i woos || eswm w5 FFnrivoimmeaarraa Premera toozs || tot | a300 = wm =" pres = Seo -ww Srmaomneata ws | wmow || 2 |S=eo =" om wer | me | ow oo w= FTaC-or20m4n6a Nwvoss3 || w1w5o0o0m0 mommgneiesn| wom 2[[0mo0ew0 | m0 | 2e[0e4oo0 | sw 2a265900 200 222 9 on Sz:Q ProwinFA TNTLEo2 em30000 7a50r0rI e 900S0 e T e e2046Rge20s Investigaotfitoxnisc effects... ...Inthewastewater Pages The following evaluation of the bacteria toxicity of he foam extinguishing agents can be concluded from these data: Highly toxic to Photobacteria are the muttirange foam extinguishing agents No. 1 and 2. `Toxic to photobacteria are the mutirange foam extinguishing agents No. 3, 4 and 5,as wel asfimforming foam agents No. 2 and 6. Lowtoxiciytophotobathcefitm-feormirngfioamaagerntesNop.1,r3(FeC-2s06,e4(FnC-2t03) and 5,thethreeproteinfoamagents, thefilm-forming flurine-proteinfoamagentsaswellas thespecial foamagentfor deep-ypanfires. _ 42 Algae muliplication inhibition test 42:1 The invesofatigaistogxicaiywteriecoondnuctsed acctoothreasdsayiguindelgine "inhibitionofthe `ell multiplication of greenalgaeScenedesmus Subspicatus CHOD" of the (German) Federal EPA dated 06.22.1962. Scenedesmus subspicatus is a single-cell autotrophic organism wih vegetative multiplication. `Stockcutoftalugaewreree s heldin noumrediaiaccs ordih ngtomtheteestgn uidet lineandtransferred toatreshmediumonceawesk. Pre-cuwteruesreetusp 3daysbeforestartingeachassaytoobtain cells n the exponential growth phase. `Theexperimentswersconductedasstatictests,Le. thetestedsubstancewasaddedonceinthe desiredconcaethnebetginrningaofttheIincuobatinon. Foreachconcentarndacotnitrool2n Erlenmeyerflaskswith 50miof utrsotlutioon wnereasatlup. The intialcll danwasssetttotyhe value between 10,000 and 15,000 cells. `Thetest cutureswereshakenonce aday.Thecellconcentrationwasdeterminedontheday0,3, 4 an7bdymeansofanelectroniccellcountingdevice.Thecallnumberswerusedtoplotthe growth curves and to calculate the mutiplication inhibition which takes Into consideration days 0,3 and & `according to the guideline. Day 7 was used for an after-observation. From the concentration-effect curves, the values of EC10 and ECS0 were determined. 001544 Investiofgtoaxitceiffoectns.s.. ...nthewastewater Pages 4.2.2Theresuoflaltgasegrowth inhibitionassayusingScenedesmus Subspweriepcutatogtethuersin Table2. "ThetableIsbultupaccording tothe sameschemeastable1.Thetoxitydeterminationof individual foamagents for Scenedemus Subspicatuswasconductedinthesamewayas thatforphotobacteria. Allfoamagentswiththeexceptionoftwoproteinfoamagentsshouldbe designatedas toxicto Scenedemus Subspicatus.Bothofthementionedproteinfoamagentshaveonlyweaktoxicty to algae.Thespecialfoamageritsfordeep-ypanfirescanbeconsidered abordercasebetweentoxic: andweatoxkicalgenyts. tisconspicuousthatoneproteinfoamagentwhichwasonlyweaklytoxic in elationtobacteria is In theupperrangeoftoxic toalgas,whileonehighlytoxiotobacteriamuti-raagenngtheafsboeaemn foundinthe midrange oftoxichyrelative toalgae. 001545 In fostETn attssessc Farest revues | EE, |B] |] canoio | wfmomenrA Nwoes || o2amde oe | = She|e .. wa | som MWFutiimrganstFFgAAe NNtoo.zd1 || 22or 9.s rFmofoommiFgA NeazgFC2|6| 1=00 0 FFiimmfoommiignFFgAA NNoodsFa|| 110650 2 9oo2sr 5ss.oo0r3zs6 230 prree 1=89 iasmm Film forminFgA No.1 355 Ffimrformneg traps | 60 36 39 | 4400 prom Sproaslefsori sMeos | as5 = =os aaom oper Net |e 0 1 wm 2g3 T+E coro communionninn g gown osstrane on =Fmerero -st 5ss0oo ps ppee 44 "" ps 52 Investigations of toxic effects... ..in the wastewater 43 Results of the Daphniashort term assay Page 10 43.1 Testorganismsinthiscasea 61024-hoursoidinstarof DaphniaMagnia.Thetoxiceffectofsubstances `contained in water onDaphnia Magnias measured asthesolution level or concentration (ppm, ppb) ofthe tested substances in which,after testing, certain percentage of Daphnia became immobile (effective concentrations for 10%and50%oftheanimals = EC10and ECS0). Tost configuration _ Ina25mibeakerlask, 5miofstandardwater (socalleddiationwater)werefiled. Th2oeidannim,als areputinlft tothemselfvore2s4hourswithoutfood.Afterthattime,theold aniarmeraemolvesd. The neonates releasedinthemeantime, werecountedandtheevapwoatrerraepltenieshedd. Now, `Simiofthe toxicantsolutionareaddedintothebeakerglass.ThisrestsInthesolutionof 10 miand thereforerepresentsthefirstlevelofdilationofthetestedsubstance inwater. Furtherlevels are obtained in the same way. Toinhthienbatiurtal swimiingactivityoftheanimalsandtosecure apossiblyequalsubstanceintake in all latrhetvesatmaeter,ial islef in darforkthennexet24shousrs; thecouonfthtesitlmnobigle instarsfollowsthereafter." Control breed Inparallel to eachassayseries, adeterminaotfitohne EC10-ivscaonlducutesdwiththe reference substance potassiumdichromate. The quantities usedinthiscaseare 0.9 - 1.9mg/lof stockwater. Thetestresultisvalidwhen ess than 10% ofDaphnianeanatesbecameimmobie inthis controlassay. 001547 Investiofgtaoxitcfiecotsn..s. ..inthewastewater Page 11 4.3.2TheresultsofDaphniashort tem testarecollectedin Table3. "Theconfigurationofthetableagainfollows theschemeusedinTable1.Alsothedeterminationofthe toxicityofinddualfoamagentstoDaphniaisconducted ustiksInprevioustestswithPhotobacteria `andgreen aigae. Allmut rangefoamagents,fim-formingfoamagentsuptothe running No. 3andthespecialfoam `agentfordesp-cypanies,aretoxicto DaphniaMagna.The remafioanmaigenntgs, Le. thefimformingFA No.3,thethreeproteinFAandtheim-formingflucrine-protein FAhavelowtoxic to Daphnia. `Generally,themuthrangeandthefim-formingfoamagentsarslesstoxi to Daphniathantothe bacteria. - 001548 re RE rere -- EES =E E[[E |E==a= rE EERENE mmrenE EiE eeelZEE(E|EBoEREo)EENa |S EmERLmAEaETT2EEfm EE1[E22 E|EI122 E2E||(EETZ|E|)E:E2 8:TT Te Enea 050+Coreotincaring 0% money fearsitn hte port Investiofgtoaxitceifeocntss.... Inthewastewater 4.4 Resultsofthefishtoxicity test Page 12 4.41 TheInvestigationofth fish toxittyaswella fishholdingandwaterqualitywere nagreementwith the OECD guidelTihenzeebsr.a barblings (Brachydanioferio) had an averabogdey lenogf3t.0h=. 0.5 cm. Th mortaltyduring the asttwoweeksofthestockholdingwas 0%. The0 contentandpHofthetestsolutionswasdetermined att =Oh,t =48hand =96h (endofthe experiment).Duringtheexperiment,therewasnonoticeablechangeoftheOzcontert. TphHofe the testsolutionswas 7.8 + 0:2atthebeginningofthetest. Th foodsupplywassuspended24hoursbeforethestatofthe test.The tempeorftahetsoulurtioen was23C 0.5C withconstantlightaerationthrough a glasscapilaryand alightcy/cldeoaf1r6/k8 hours. Ineachconcentratleivoeln, 10fishes wereputinthe 10 tertestsolution n a 15Iker ful glassbowl understatic testconditionsandthe numberofdeaddeterminedafter24, 48,72and96hours. Uncertaintiesduringtheexperweir mreceorndetd. The LC, were calcwuithlaacotmpeutedr program accotrodLicihtnfiegld and Wilcoxon 1949; LG, and LC,resdiurectllyfrtomtehetdest. 4.4.2 The resouftlhetfissh toxicty test are compiledin Tabl4e. The result representation and determinationofthe toxicty ofindividualfoamagents to fish again followsthebactetesrt(iseae 4.1.2). Clearly,toxic to fishareonlymuti-rangefoamagentsNo.2 and 4. Theremaining muti-rangefoam agents No. 1 and3,aswell asthefim-formingfoamagents No.1 and 2canbeassitgoantoexdi/ weaklytoxictransitrainogen.Weakly toxic to fisharethefim forming foamagentsNo.3 (FC-206,4 (FC-20), 5.and6,theprotein foamagents No. 2and3,as wlasthe specialfoamagentfordeapry fires. Thefilm-formingfoam agent No. 1aswell asthefim-forming fluorine-protein foamagentare generaly non-toxictofish, 001550 Torttomonionsores Ties Acustiotes LCseLOIehour)aoosScomcbrio in5elEghrs ounsors4 F aw E Commviee oifgsn MMaaannggoe FFAA KNooz 10@0 o= ss aas0t Matango FA FinfominfgA Noa oz pe pa 100 100 100 107 om asm FinfomioFgA Not 01 100 10s ser [ry ------ n J I0) TPo aamsso SpJ eci FA 1080 11000000 wPey 22004m1 FimfomioFgA NoaFz 4000 00 0 2m FFimmttoommiingaFtA Nfoogs || 7207s5 2E0o)0 21e0z0 2P6rm) FibmofoomipngfoueNperzoan [1[0e00s0 | 10u00n0 sTa00m00 222m ProsnfA 82 Not [doo [iow Too00 2 [RASnE voHopresomcrcetotss dobi2o0%fe oit ci aa Pago 120 ic) n rFnagnes a" "0 ""00 awz 2a08 27 2270 225 2 Investiofgtaocteiffeoctns.s.. Inthewastewater Pago 12 45 Deterofbmiocihenmicaaldtegiradoabinty InTable5, thefiguresofthechemicaloxygen demand(GOD)andthebiochamicaloxygen demand duringthe fiveconsecutive days (BOD5),expressedinmg O,per terof water, werecollectedfor 3% `appsollutiionsocftahe ftoaimagoennts. Thelevelofthebiochemicaldegradabilyofthetestedsubstancescanbesufficient accurately datroomtrheramtiooif GOnDtoeBOdD. InandealcasetheGOD to BOD Swouidbe 1:1. Such ratiowouddescrib annearlyquantitativebiodegradationof a testedsubstance. RatoflCOoDsto BOD 5of1:0.5signalaninsufficientbiochemicaldegradation. ThevaluespresentedinTable 5pointtothe insufficientbiochemicaldegradationofthemutirange foamagents No. 1and 4 aswel asthefim-formingagent No.3 (FC-206). Duringthecategorization oftheconcentratesofthes foamagentsinto awaterendangeringcatagory,thecharacteristicsof insufficientbiochenicaldegradabifyfsconsideredas ogative ("Malus")andresultedinassignment 10thanexthigherwaterendangecarteignorgy (5004.7). 001552 : sss ms DandBOD'S of inguishing agents. - Table5: ee =[ .CO1T2E| 0| | | Sn g===E- e awlol me|Be | J| vee | | fmaeaver =- = = mle | |e ||== ==arrooem ot mwm=o oo|=mm || vem nEem =m |e || ||a=e. |= N==Aos rron| = o21mw9m0o0 o[me[own49 mom || v|vaoPekm) |e ||1 w|m=en.r == 2gE 4 wor menT | mo fwemews]| w Investigationsoftoxiceects... ...inthewastewater Page 14 4.6 Determination of the water endangering figures and classification of the tested foam extinguishing `agent concentrates ntowater endangering categories. 46:1 As already explained in the introduction, the "Commission for Evaluation of Water Endangering Materials"consideredtheactatoxic toselectedtestorganismsas aprimarycriteriaforclassifica: tionof asubstanceor a substancemidureinto awaterendangeringcategory. AfurtherImportant evaluation criteria was the biochemical degradabilty of the substance or substance mixture under consideration. Anerconsultawittihsoonmesofthe membersofthe "Commissifoonr EvaluationofWater EndangerIngMaterials"regarding the classification of 16foam extinguishing agent concentrates, the determinationof themammalstoxicity (LDSOora,hasbeen waived.Instead,Inaddition totheprescribed consideration of the - acute bacteria toxicty and the - acute fish toxicity, theacutetoxicties to ~aigae and -water flea were taken into consideration during the classification of the tested foam extinguishing agent `concentrates ino water endangering categories. 46:2 InTable6,theevaluation figuresoftheacutetoxicsofthefoamextinguishingagentstothetest organisms,arepresentedincolumns 2through 5. Accordingtothe methodsemployedbythe "Commission for EvaluationofWater Endangering Materials", the arithmetic averages of the evaluation figures: result n the so called water endangering number (WEN) of the material under consideration (6th column). Fromthewaterendangering numbersdetermined inthis way,thewater `endangering categories (WEC) are derived according tothe following scheme: WENGandhigher WEN401059 WEN20tp39 WEN 1.9andlower -WEC3 -WEC2 -WEC1 -WECO `The classificationofthe tested foam extinguishingagentconcentrates Into the water endangering catagories can be taken from the last column of Table 6, 001554 Tae 1)Dcotromiinasionoofvyatseoadranaccisn,augroes,(wYEr)fa5sa3nnrhh.saverageof heoan urs 2 he estingFcowaarsensdebrccaeiceo(nWC Page"oe 14a . Foameangushingagers Esrato rAeofonr ekwoilkyagsa. Fon anadanrguing | endaWngaearring Ton|| cass HEC) MMoultiriaangnogFFeAA NNoo..45 5587 NA50 aas MuliranFgoA No.2 62 4 a7 5502 22 9 2 MMuuttiirraannFFggooAA No1. No.3 6502 FimiormiFnAg No.2 40 40 40 2 2 40 2 FiFmi-mo-moinmginFFgAANoNo..16 a4s5 541 437 2470 423 22 FimiormiFnAg No.4FC203| 32 8 45 30 39 1 Fim-ominFgA No.5 `Special FA 32 37 48 40 Fim-ormiFnAg No.3FC-208|| 29 8 29, 28 a7 a7 1 1 27 3s 1 PPooiiosnnFFAA NNoo..23 31s9 3303 11 o FiPmu-oobmniFAngfuornNeoptroten |228.1 a42 2247 2200 2267 1 1 g2& 1- a4 Investigationsofoxiceffects... ...inthewastewater Page 15 4.6.3 Underthapressureofthegeneralactiveenvironmentalconsciousness,thequestiondiscussedtodatos: `Shouldclassificationofthesubstanceconcentrateinto awaterendangeringcategorybe `basedonacute toxicitytothe mostsensitivememberof afoodchain? `Shouldthisbethecase, hecategorizationofthe 16foamextinguishingagentconcentratespresented inTable 7 intowaterendangeringcategorieswouldbetheresult. 47 Thebiochemicaldegradabiltyofthe - mutif-oamrmaaternialgNoe.1 and -muirfoaamagnentgNoe. 4 1snotsufficient. "Thisfactleadsto adowngradingoftheconcentratesfromthewaterendangeringcategory 2(see `Table 6)tothewater endangceatregiorny3g. Incaseoftheinsufficientlybiochemicallydegradablafim-formingfoamagent No.3,the downgrading takesplacefrom the waterendangering category 1(see Table6)tothe waterendangering categor2y. 001556 Ten) TOtoneoomtsritnorgncoiafsetsia:aandaonvgaetin es (WEF)bs onth vaio endangeringctogores(EC) urs ohomostsn page 150 Foamaingisting ge EoTre or RoT R oa | Tear | sncaangrong MultiagFeA No.2 MutiangeFA No.1 6602 MutiangFeA No.5 58 4"57 4a0 6a20 33 a | Na 58 2 MuMtuitriantgaengFFAeA NNoo..34 5572 5510 Fimiorming FA No.6 45 51 4430 5527 22 27 51 2 PFiomdaorFmAing FA NNoo..22 FimdormiFnAg No4. 24s4 5409 22 5 48 2 FFiammifoormmiiFnFnAgAg NNoo.1.3FC: Fimioming FA No.5 3as 2470" 4a8 22 41 29 4s 2 Fimo.fuorneproten Special FA || 2a17 04 2a4 2208 44 22 2 PPoowwonnFFAA : NNoo..13 Ba4sa i a2s7 a220 11 Investigationsoftoxic effects... ...inthewastewater Pago 16 5. Fcinoanlsiderations Presented ~dataofthe cutstoctyoftheapplicationsolutionsoffoamextinguishingagentstowaterorganisms -dataconceringbiochemicaldogradabtyoffoamextinguishing agents -cclaatsseigfoirciaetisobnaosfetdhoentestedfoamextinguishingagentconcentratesinothewater endangering arthmeticaveragesofthe evaluation figures of theact toxicities evaluationfiguresoftheacutetoxitles tothemstsensitiveorganism n cachcaso have10bochacbkyethde"CommissionforEvaluation ofWaterEndangeringMaterials". cTohnesiCdoemrmeidscsoimopnlweitlaeovraliuatoeatdhoetfofxuirctihteyrdiantveasetisgtaatbiloinssh.edbythearmyandindicate ifit shouldbe t"iTohneoefxafmoianmateixotninwgiuliaslhsiongpargoveindtes andteottehrmeiwnaatteironecndoanncgeemriinnggctahteapgorr ieso . cuse edfd ortu hecr lase sifis ca- lEsapweocfiatlhleytEhuerloatpteearnreCsoumlmtiusnoiftiyntsetraersttiintigsilnik1e9ly9t2o. seta precedencethatcouldaffectthecommercial 001558