Document GK6RZyZ812vozNNEq4LRND4Km
ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA)
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, oras a component ofFC-600. (1-Octanesulfoniaccid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3)
Remarks: The 3M productionlotnumber was not noted. The test sample isFC-600 (SCAS Treated Effluent*)C.urrentinformationindicates FC-600 isa mixtureof 1.0% PFOS, 81.20% water,12.00% diethylene glycolbutylether,1.00% sodium octylsulfate2,.00% propane Sultone foamer, 1.00% sodium decylsulfate0,.85% xanthan gum, 0.1% N-(3chloroallyhle)xaminium chloride0,.80% starch,and 0.05 % benzotriazole.
The followingsummary applies to a mixture with incompletely characterizedconcentration of impuritiesthatwas treatedin a SCAS reactor.Date may not accuratelyreflectthe toxicityof the fluorochemical component of the testsample.
Thisstudy isthethirdof 3 daphnia studiesconducted to determinethe toxicitoyf FC-600 afterbeingtreatedthrougha SCAS reactor.This study isthe controlforthe FC-600 SCAS Treated Effluensttudy. Itused the supernatantofa mixed microbialculturethatinitialcloyntainedno FC-600. The effluenwtas collectedafter4 hours oftreatmentina SCAS reactor. The inoculumused came from the Metro Wastewater Treatment Plant,St. Paul,MN.
METHOD:
Method: Not noted. Type: Staticacute GLP: No Year completed: 1977 Species: Daphnia magna Supplier:Not noted. Analyticalmonitoring:Dissolvedoxygen Exposure period:48-hours Test organism age: Not noted. Statisticamlethod: EC5o calculatebdy graphingdataand by probit analysis. Test conditions:
Dilutionwater: Carbon-filterewdellwater,aerated. Dilutionwater chemistry:
Dissolved oxygen: 9.4 rrig/L Lighting:Not noted.
Stockand testsolutiopnreparatioVno:lumetvolumdeirecatdditiotno testvessels. Exposure vessels: Not noted. Number of replicates:two Number of daphnids per replicate:10 Number of concentrations: six Water chemistry during the study:
Dissolved oxygen (final): 7.5mg/L (5.6% exposure) 6.5mg/L (100% exposure)
RESULTS
Nominal concentrations:5.6%, 10%, 18%, 32%, 56%, and 100%
Element values: 48-hourEC50 = 15 (13 - 18) %
Element values based on nominal concentrations
Remarks: Testingwas conducted on thesupernatantcreatedfrom a SCAS studyas describedintheTest Substance Remarks field.The values reportedapplytothatsolutionand notthetestsubstance.
CONCLUSIONS
The SCAS treatedeffluent48-hour EC50 was determined to be 15% witha 95% confidenceintervaolf 13 to 18%.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking= 3.This studylacksdescripbonofthe methodology used, reportingoftestconditionsa,nd informationon characteristiocfsthe effluenatfterSCAS treatment.
REFERENCES
This studywas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, 1977.
OTHER
Last changed: 6/28/00
-,7.40101
ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET
Type Test 99 Hours - To,-ERA4kC STW
Wi4luiA K"DA, Test Organism P-C-b-.,4L4;rauw
Date Starte(@-
5@IR177
Material Tested Avg. Weight Date Completed
Fe- (000
Se_A 5 CotaTIZOL Avg. Size
Time Started
Plotted LCSO
Dilution Water Carb)On Filtered - Well Water (AAL'- ACRO CTC 1> -m
14
LSUPCPIPA-T@
2W hrs
4,9hrs
Conc.
%
D.0
%
D.0
Survival PH ppm /Survival PH p pa
C-6 7-
/190
tod7,
it hrs
%
D.0
Survival pH ppm
/00.
7.2
96hrs
%
D.0
Survival pH ppm
72
7.2
Average Temperature Comments *.4r
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100
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40
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80
90
100
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