Document GK634mkM7b1kd6XGKqEEo6X8q

TRADE SECRET AR226-3167 DuPont-4725 Study Title H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas Laboratory Project ID: DuPont-4725 AUTHOR: Robert A. Hoke, Ph.D. STUDY COMPLETED ON: September 26, 2000 PERFORMING LABORATORY: E.I. du Font de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 Cwnpany Sanifeed. Does not contain TSCA CW __ H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas Substance Tested: Synonyms/Codes: STUDY INFORMATION Haskell Number: Composition: 24516 DuPont-4725 Known Impurities: Physical Characteristics: Stability: Sponsor: In-Life Initiated/Completed: Pale to medium tan waxy solid The test substance appeared to be stable under the conditions of the study. E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. September 11, 2000/September 15, 2000 not CB8 2- H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4725 SUMMARY The acute toxicity of H-24516 to the fathead minnow, Pimephales promelas, was determined in an unaerated, 96-hour, static test. The study was conducted with 4 concentrations of H-24516 and a dilution water control at a mean temperature of 22.1 C (range of 21.9-22.1 C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and the 1,10, 100, and 1000 mg/L test concentrations were clear and colorless with undissolved H-24516 visible in all of the test concentrations at test start. All water quality parameters were within acceptable limits during the exposure (Table 1). Exposure of fathead minnows to the water control and nominal H-24516 concentrations of 1, 10, 100, and 1000 mg/L resulted in 0, 0, 0, 0, and 100% mortality, respectively, at the end of 96 hours (Table 2). No mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 100 mg/L. The lowest mean, nominal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-24516 concentrations were used for calculation of the LC5o value. The 96-hour LC5o value, based on nominal concentrations of H-24516, was 316 mg/L. The results are summarized as follows: Nominal concentrations of H-24516, mg/L 96-hour LC5o for H-24516, based on nominal concentrations, mg/L dilution water control, 1, 10,100, and 1000 316 (100 to 1000) H-24516 exhibited low concern for aquatic hazard1' in an unaerated, 96-hour, static acute test using the fathead minnow, Pimephales promelas. " Stephan, C.E. (1997). Methods for calculating an U^o. Aquatic Toxicology and Hazard Evaluation, Proceedings of the First Annual Symposium on Aquatic Toxicology, ASTMSpecial Publication 634 (F'.L. Mayer and J.L. Hamelink, Eds.), pp 65-84. Philadelphia. b Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASTMSTP 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia. -3- Company Sanitized. Does not ecmfain TSCA CBS H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4725 CERTIFICATION I, the undersigned, declare that this report provides an accurate evaluation of data obtained from this study. - Work Compieted by: ^nui/ilf^^anAHJAkhiiSSllSlLi ' ' TLaaluInriaeTD-t.Bnonuncrhllelle Associate Scientist Wort Completed by: ^^me/^l-^-^SsSSia^y Daniel XC.Peterson Associate Scieatitt Issned by Study Director:; ^wu/- ^- RflbertA.Hoke,HiJ). Senior Research Scientist ^^^OOD iMte Date -ff& Sy^n^u -^06 Date -4- CQmpany SanlSteed. Does not eonteSn TSCA C8( H-24516: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4725 TABLE 1 DISSOLVED OXYGEN AND pH OF CONTROL AND H-24516 TEST SOLUTIONS Nominal H-24516 Concentration (mg/L) Test Initiation (0 hours) Dissolved Oxygen (mg/L) PH Test Completion (96 hours) Dissolved Oxygen (mg/L) PH Water Control 8.7 7.8 7.0 7.3 1.0 8.7 7.7 7.0 7.4 10 8.7 7.7 7.1 7.4 100 8.7 7.9 6.9 7.5 1000 8.4 7.5 7.2s 7.6-1 a Measurement taken at 48 hours due to total mortality TABLE 2 MORTALITY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24516 Nominal H-24516 Concentration (mg/L) Water Control ' 1.0 10 100 1000 Number Dead/ Number at Study Start 24 Hours 48 Hours 72 Hours 96 Hours 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 , 0/10 10/10 0/10 ' 0/10 0/10 0/10 10/10 0/10 0/10 0/10 0/10 10/10 Company Sanitized. Dees not contain TSCA CBI -5-