Document G6o38gd4XOko4BK8eEKye7eZY

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component ofFC-201 F-X. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 1.The testsample isFC201 F-X (F-11129). Currentinformationindicatesitisa mixture3% PFOS, 36% diethyleneglycolbutylether,36.6% water,8.25% acrylifcbamer,7% sodium octylsulfate6,% sulfonatedalkylethersalts,3% tdethanolamine,and 0.15% toluoltdazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurateiy reftct the toxicityof the fluorochemical component of the testsample. METHOD Method: OECD Guideline209 GLP: No Year Completed: 1992 Analyticalmonitoring: Dissolvedoxygen concentrationst,emperature. Statisticamlethods: Not applicable. Test organism source: Activatedsludgemixed liquocrollectefdrom the MetroWastewater TreatmentPlant,St.Paul,MN Test condition: Dilutionwater: MilliporMeilli-QTwmater Syntheticsewage: perOECD method Reference and testsolutionpreparation:A 500 mg/L stocksolution ofthe referencesubstance,3,5-dichlorophenowla,s prepared. The pH ofthe referencesolutionwas measured to be 7.8. Test substance was added directltyothetestvessels. Test vessels: Not given Number of concentrations: one plusblank controls,allinduplicate Temperature: 17.7- 19.0OC Totalsuspended solids and pH on day of testing: 3.4g/L (TSS in final1:.36 g/L. InitiaplH 7.5 and finalpH 7.5. Element Basis: Respirationinhibitioans determined by oxygen consumption. Remarks: Uptake ratescorrectedto 20*C forcalculations. RESULTS Nominal concentrations: Blank controlsand 1000 mg/L, allinduplicate Elementvalues: 30-minuteEC50=>1000mg/L 3-hour EC50 = > 1000 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on a mixtureas described inthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 3-hour EC50 foractivatedsludge respiratioinnhibitiownas determined tobe >1000 mg/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking3.Several pages are missingfrom thisstudy. Sample puritywas not propedy characterizedand the study lacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Kl 126-1,1992. OTHER Last changed: 6/26/00 Type Test E-NVIRCNME-NTAL LASCRATORY ACTiVATE-:) SLUE)GE MIXED UQUOR f-@(-TI" 'ITf,i@D SLVO(DL (Z'LSP)eZ/;-riON LR K,112(.-I Date "S/23/9?Analyst KSM PagO I Of -7 !,Jll,'3rTiVN Tts-rF*26'ri ACTIVATED SLUT)GE MIXEP LlC2UOg Date Obtained -@./2-13-92. Date Used 3 Mixed Uquar Suspended Solids (MLSS 'I), g/L Adjusted to MLSS in finaltest solution g/L InitiaplH -7.5- (lsttestsoin.prepared) Fmal pH Comments g/L (lastsoin.prepared) TEST SUBSTANCE Sample Description 7- c- 2 0 t InitialpH of highest conc. tested ( IOCC =@/L-) Comments Li aitI) 112)e, Lc-rI Adjusted to pH REFERENCE SUBSTANCE Sample Description initiaplH of stock solution 5-0C mg/L) -7. Comments Adjusted to pH EXPOSURE CONDMONS Test Duration Tes4*7emperature Range: c Comments -7 LFI ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION Test Substance: ;)ol7 Sample Description: bkwr4 Analyst: Date: -111 L,'-)T Test Conc. -ma/L mg added ger 500 mL mL MO(l) water mL syn. sewage 22r 500 mL feed per 500 mL mL microbial inaculum per 500 mL @000 -A -)C40 occ- 5,oo TotalVolume mL 500 500 o -b 7.-) (1) MilliparMeilli-ORhigh puritywater ENVIRONMENTAL LABORATORY OECD ACTIVATED SWDGE RESPIRATION CALCULATION OF RESULTS INHIBITIONTEST #209 Test Substance: FC -201 F-X Analyst:IM 30-Minuto Exposure Dissolvd Oxygen Concentrati s, mg/L mq/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp,'C BKI 8.7 8.5 8.1 7.8 7.5 7.2 6.9 6.6 6.2 5.9 5.5 17.7 iooo.oo 2.7 2.4 1.9 1.4 0.9 0.4 - - - - - 1 18.5 iooo.oo 4.1 3.6 3.3 2.8 2.4 1.8 1.4 0.9 0.5 18.6 R (1) 0.647 1.000 0.900 BK2 8.3 7.9 7.7 7.4 7.0 6.7 6.4 6 5.3 5.0 % diffincontrols(4): 0.5 EC50 (95% Cl):>1000 mg/L 17.9 3-Hou Exposure ma/L 0.5 i.o ............ ... BKI 7.9 7.7 iooo.oo- 8.3 8.0 iooo.oo 8.8 8.5 Dissolvd Oxygen Concentrati s, mg/L 1.5 3.0 3.5 4.0 4.5 5.0 5.5 Taffp.-C . . ....................... 7.4 7.1 6.8 6.4 6.1 5.7 5.4 5.1 4.8 18.1 7.6 7.3 6.8 6.3 5.9 5.4 5.1 4.7 4.3 19.0 8.1 7.9 7.5 7.2 6.9 6.5 6.1 5.7 5.4 I- 18.1 0.659 R 1) 0.658 0.838 0.690 BK2 8._6 8.4 8. % diffin contro Is(4): 0.8 7.4 7.1 6.7 F6.5 1 6.1 5.8 5.3 EC 5Q (95% Cl):>1000 mg/L 18.2 0.66.7 (1) R= Oxygen uptake rate(mg 02IL-min) (2) Ra--R adjustedto20*C= JR/o.Ex] (3) % Inhibitioni=(RaCm)-RaS/(RaCm)1100 (4) % dgierenceIncontrols:IlRaBki-RaBk2itRaCm]100(Two controlmsust be within16% oleach other.) (5) Mean adjustedR, controls[-R-aBkl+RaBk2y2 1.072 RaCm. -mean adjustedR,coratols Sk. blankcontrolS.. sample Tobs- sample tomp x- Tobs-20'C